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Anti-polysaccharide Antibody

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https://www.readbyqxmd.com/read/28069358/pneumococcal-polysaccharide-vaccination-administered-early-after-neurotrauma-or-neurosurgery
#1
Anna Ljunghill Hedberg, Karlis Pauksens, Per Enblad, Jessika Söderberg, Björn Johansson, Helena Käyhty, Jan Sjölin
OBJECTIVES: Pneumococcal vaccination is recommended to lower the risk of posttraumatic meningitis, and early vaccination may be of importance. After both trauma and central nervous system injury, immune-suppression may occur, which could affect T-cell function and the response to T-cell dependent vaccines. We therefore aimed to investigate the response to early vaccination with a T-cell independent pneumococcal polysaccharide vaccine (PPSV). METHODS: Thirty-three patients with basilar skull fracture and 23 patients undergoing transsphenoidal pituitary gland surgery were vaccinated with PPSV within 10days after neurotrauma or neurosurgery...
January 6, 2017: Vaccine
https://www.readbyqxmd.com/read/28049369/immunocytochemical-detection-of-rhamnogalacturonan-ii-on-forming-cell-plates-in-cultured-tobacco-cells
#2
Ye Zhou, Tatsuya Awano, Masaru Kobayashi, Toru Matoh, Keiji Takabe
Rhamnogalacturonan II (RG-II) is a region of pectin macromolecules that is present in plant primary cell walls. The RG-II region serves as the site of borate cross-linking within pectin, via which pectin macromolecules link together to form a gel. In this study, we examined whether RG-II is present in the cell plate, the precursor of primary cell walls that forms during cytokinesis. A structure inside dividing cells was labeled with a rabbit polyclonal anti-RG-II antibody and detected by immunofluorescence microscopy...
January 4, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28031178/otitis-prone-children-produce-functional-antibodies-to-pneumolysin-and-pneumococcal-polysaccharides
#3
Lea-Ann S Kirkham, Selma P Wiertsema, Karli J Corscadden, Tulia Mateus, Gemma L Mullaney, Guicheng Zhang, Peter C Richmond, Ruth B Thornton
BACKGROUND: The pneumococcus is a major otitis media (OM) pathogen but data are conflicting on whether otitis-prone children have impaired humoral immunity to pneumococcal antigens. We and others have shown that otitis-prone and healthy children have similar antibody titres to pneumococcal proteins and polysaccharides (vaccine and non-vaccine type), however the quality of antibodies from otitis-prone children has not been investigated. Antibody function, rather than titre, is considered to better correlate with protection from pneumococcal disease...
December 28, 2016: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/27974829/evidence-for-a-los-and-a-capsular-polysaccharide-in-capnocytophaga-canimorsus
#4
Francesco Renzi, Simon J Ittig, Irina Sadovskaya, Estelle Hess, Frederic Lauber, Melanie Dol, Hwain Shin, Manuela Mally, Chantal Fiechter, Ursula Sauder, Mohamed Chami, Guy R Cornelis
Capnocytophaga canimorsus is a dog's and cat's oral commensal which can cause fatal human infections upon bites or scratches. Infections mainly start with flu-like symptoms but can rapidly evolve in fatal septicaemia with a mortality as high as 40%. Here we present the discovery of a polysaccharide capsule (CPS) at the surface of C. canimorsus 5 (Cc5), a strain isolated from a fulminant septicaemia. We provide genetic and chemical data showing that this capsule is related to the lipooligosaccharide (LOS) and probably composed of the same polysaccharide units...
December 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27960627/comparative-evaluation-of-a-newly-developed-13-valent-pneumococcal-conjugate-vaccine-in-a-mouse-model
#5
Chulmin Park, Eun-Young Kwon, Su-Mi Choi, Sung-Yeon Cho, Ji-Hyun Byun, Jung Yeon Park, Dong-Gun Lee, Jin Han Kang, Jinhwan Shin, Hun Kim
Animal models facilitate evaluation of vaccine efficacy at relatively low cost. This study was a comparative evaluation of the immunogenicity and protective efficacy of a new 13-valent pneumococcal conjugate vaccine (PCV13) with a control vaccine in a mouse model. After vaccination, anti-capsular antibody levels were evaluated by pneumococcal polysaccharide (PnP) enzyme-linked immunosorbent assay (ELISA) and opsonophagocytic killing assay (OPA). Also, mice were challenged intraperitoneally with 100-fold of the 50% lethal dose of Streptococcus pneumoniae...
December 14, 2016: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/27931102/mannose-receptor-mediates-the-immune-response-to-ganoderma-atrum-polysaccharides-in-macrophages
#6
Wen-Juan Li, Xiao-Fang Tang, Xiao-Xue Shuai, Cheng-Jia Jiang, Xiang Liu, Le-Feng Wang, Yu-Fei Yao, Shao-Ping Nie, Ming-Yong Xie
The ability of mannose receptor (MR) to recognize the carbohydrate structures is well-established. Here, we reported that MR was crucial for the immune response to a Ganoderma atrum polysaccharide (PSG-1), as evidenced by elevation of MR in association with increase of phagocytosis and concentrations of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in normal macrophages. Elevation of MR triggered by PSG-1 also led to control lipopolysaccharide (LPS)-triggered inflammatory response via the increase of interleukin-10 (IL-10) and inhibition of phagocytosis and IL-1β...
January 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27930983/sargassum-fusiforme-polysaccharides-inhibit-vegf-a-related-angiogenesis-and-proliferation-of-lung-cancer-in-vitro-and-in-vivo
#7
Huiling Chen, Ling Zhang, Xiange Long, Peifei Li, Shengcan Chen, Wei Kuang, Junming Guo
Sargassum fusiforme (Harv.) is a brown alga belonging to the Sargasaceae family. The Sargassum fusiforme polysaccharides (SFPS) have demonstrated good anti-tumor and immunomodulatory activity. However, the underlying mechanisms of its anti-tumorigenesis, especially the anti-angiogenic activity is yet to be established. In the present study, we attempted to determine the effects of SFPS on the human lung adenocarcinoma SPC-A-1 cells and its xenograft model. The results showed that SFPS provides a concentration-dependent inhibition of SPC-A-1 cell proliferation in in vitro and the tumor growth in in vivo studies...
January 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27895641/pneumococcal-polysaccharide-vaccination-elicits-igg-anti-a-b-blood-group-antibodies-in-healthy-individuals-and-patients-with-type-i-diabetes-mellitus
#8
Wendelin Wolfram, Kai M T Sauerwein, Christoph J Binder, Nicole Eibl-Musil, Hermann M Wolf, Michael B Fischer
HYPOTHESIS: Blood group antibodies are natural antibodies that develop early in life in response to cross-reactive environmental antigens in the absence of antigen encounter. Even later in life structural similarities in saccharide composition between environmental antigens such as bacterial polysaccharides and blood group A/B antigens could lead to changes in serum levels, IgM/IgG isotype, and affinity maturation of blood group anti-A/B antibodies. We addressed the question whether immunization with pneumococcal polysaccharide (PnP) vaccine Pneumo 23 Vaccine "Pasteur Merieux" (Pn23) could have such an effect in patients with type I diabetes mellitus (DM I), an autoimmune disease where an aberrant immune response to microbial antigens likely plays a role...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27885841/the-utility-of-saliva-for-the-assessment-of-anti-pneumococcal-antibodies-investigation-of-saliva-as-a-marker-of-antibody-status-in-serum
#9
Jennifer L J Heaney, Anna C Phillips, Douglas Carroll, Mark T Drayson
CONTEXT: Salivary antibodies may act as non-invasive marker of systemic immunity enabling assessment of vaccination and protection against bacterial infections. OBJECTIVE: To assess if levels of anti-pneumococcal (Pn) antibodies in saliva reflect concentrations in serum and determine whether saliva can accurately identify protective concentrations in serum. METHODS: IgG, IgA and IgM antibody levels in paired saliva and serum samples were measured against 12 Pn polysaccharide antigens in 72 healthy adults...
December 12, 2016: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/27866926/evaluation-of-humoral-immunity-profiles-to-identify-heart-recipients-at-risk-for-development-of-severe-infections-a-multicenter-prospective-study
#10
Elizabeth Sarmiento, Maria Jaramillo, Leticia Calahorra, Juan Fernandez-Yañez, Miguel Gomez-Sanchez, Maria G Crespo-Leiro, Maria Paniagua, Luis Almenar, Monica Cebrian, Gregorio Rabago, Beltran Levy, Javier Segovia, Manuel Gomez-Bueno, Javier Lopez, Sonia Mirabet, Joaquin Navarro, Juan Jose Rodriguez-Molina, Eduardo Fernandez-Cruz, Javier Carbone
BACKGROUND: New biomarkers are necessary to improve detection of the risk of infection in heart transplantation. We performed a multicenter study to evaluate humoral immunity profiles that could better enable us to identify heart recipients at risk of severe infections. METHODS: We prospectively analyzed 170 adult heart recipients at 8 centers in Spain. Study points were before transplantation and 7 and 30 days after transplantation. Immune parameters included IgG, IgM, IgA and complement factors C3 and C4, and titers of specific antibody to pneumococcal polysaccharide antigens (anti-PPS) and to cytomegalovirus (CMV)...
October 17, 2016: Journal of Heart and Lung Transplantation
https://www.readbyqxmd.com/read/27707941/development-of-a-colloidal-gold-based-immunochromatographic-strip-for-rapid-detection-of-klebsiella-pneumoniae-serotypes-k1-and-k2
#11
L Kristopher Siu, Yu-Kuo Tsai, Jung-Chung Lin, Te-Li Chen, Chang-Phone Fung, Feng-Yee Chang
In this study, a novel colloidal gold-based immunochromatographic strip (ICS) containing anti-Klebsiella pneumoniae capsular polysaccharide polyclonal antibodies was developed to specifically detect K. pneumoniae serotypes K1 and K2. Capsular polysaccharide K1 and K2 antigens were first used to produce polyclonal anti-K1 and anti-K2 antibodies. Reference strains with different serotypes, nontypeable K. pneumoniae strains, and other bacterial species were then used to assess the sensitivity and specificity of these test strips...
December 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27671512/immunogenicity-of-23-valent-pneumococcal-vaccine-in-children-with-systemic-lupus-erythematosus
#12
Soheila Alyasin, Marzieh Adab, Asieh Hosseinpour, Reza Amin, Maryam Babaei
BACKGROUND: Systemic Lupus Erythematosus (SLE) is a chronic autoimmune disease which is characterized by B-cell abnormality and auto-antibody generation. Since bacterial infections are the most important causes of mortality in these patients, pneumococcal vaccination is recommended for children with SLE. OBJECTIVE: To investigate humoral immunity and specific-antibody formation in response to a 23-valent polysaccharide pneumococcal vaccination in SLE children and asthmatic control group...
September 2016: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/27650380/immunogenicity-and-protective-immunity-against-otitis-media-caused-by-pneumococcus-in-mice-of-hib-conjugate-vaccine-with-psaa-protein-carrier
#13
Zeyu Chen, Rong Guo, Jianghong Xu, Chuangjun Qiu
This study evaluated the immunogenicity and protective immunity of a Hemophilus influenzae b (Hib) polysaccharide conjugate vaccine with the pneumococcal surface adhesin A (PsaA) protein carrier in young mice. The Hib polysaccharide was conjugated with the rPsaA protein carrier, which was produced using recombinant DNA technology. A total of 15 young mice aged 3 weeks to 5 weeks were immunized with the conjugate vaccine, and another 15 young mice of the same age were immunized with the licensed Hib-tetanus toxoid (TT) vaccine...
September 20, 2016: Frontiers of Medicine
https://www.readbyqxmd.com/read/27592080/pleurotus-citrinopileatus-polysaccharide-induces-activation-of-human-dendritic-cells-through-multiple-pathways
#14
Ken-Ichiro Minato, Lisa C Laan, Akihiro Ohara, Irma van Die
Many edible mushrooms have become attractive as "health foods" and as source materials for immunomodulators. To increase our insight in the immune-modulatory properties of a polysaccharide of the oyster mushroom Pleurotus citrinopileatus, PCPS, we analyzed its effects on the function of human dendritic cells (DCs). We showed that PCPS induces upregulation of the surface maturation markers CD80, CD86 and HLA-DR on DCs, indicating its potential to induce DC maturation. In addition, PCPS stimulates DCs to secrete the pro-inflammatory cytokines TNF, IL-1β, IL-6 and IL-12, as well as the anti-inflammatory cytokine IL-10, and induces enhanced mRNA levels of the chemokines CCL2, CCL3, CCL8, CXCL9, CXCL10, and LTA...
November 2016: International Immunopharmacology
https://www.readbyqxmd.com/read/27579859/agglutination-by-anti-capsular-polysaccharide-antibody-is-associated-with-protection-against-experimental-human-pneumococcal-carriage
#15
E Mitsi, A M Roche, J Reiné, T Zangari, J T Owugha, S H Pennington, J F Gritzfeld, A D Wright, A M Collins, S van Selm, M I de Jonge, S B Gordon, J N Weiser, D M Ferreira
The ability of pneumococcal conjugate vaccine (PCV) to decrease transmission by blocking the acquisition of colonization has been attributed to herd immunity. We describe the role of mucosal immunoglobulin G (IgG) to capsular polysaccharide (CPS) in mediating protection from carriage, translating our findings from a murine model to humans. We used a flow cytometric assay to quantify antibody-mediated agglutination demonstrating that hyperimmune sera generated against an unencapsulated mutant was poorly agglutinating...
August 31, 2016: Mucosal Immunology
https://www.readbyqxmd.com/read/27543805/purification-and-characterisation-of-anti-pneumococcal-capsular-polysaccharide-igg-immunoglobulins
#16
Antony R Parker, Emma Lock, Asma Iftikhar, Richard Barber, Phil D Stubbs, Stephen Harding, Gregg L F Wallis
OBJECTIVES: The production of reference materials for quantifying pneumococcal antibody concentrations relies upon large scale vaccination. An alternative simple, reproducible protocol has been developed for the affinity purification of 23 serotype anti-pneumococcal capsular polysaccharide (PCP) IgG immunoglobulins. DESIGN & METHODS: The purification protocol utilised IgG fractionation, capsular polysaccharide (CPS) adsorption, and affinity chromatography using Pneumovax®-Sepharose...
January 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/27517529/hyaluronate-death-receptor-5-antibody-conjugates-for-targeted-treatment-of-liver-metastasis
#17
Hwiwon Lee, Beom-Ju Hong, Jeong Ho Lee, Sujin Yeo, Hoe-Yune Jung, Junho Chung, G-One Ahn, Sei Kwang Hahn
The liver is the most frequent site of metastasis with a 5-year survival rate of only 20-40%. In this work, hyaluronate (HA)-death receptor 5 antibody (DR5 Ab) conjugate was synthesized as a dual targeting therapeutic agent to treat liver metastasis. Dual targeting was achieved by DR5 Ab, a humanized agonistic monoclonal antibody binding to DR5 frequently overexpressed in many kinds of cancer cells, and by HA, a natural polysaccharide binding to HA receptors highly expressed in both the liver and cancer cells...
September 12, 2016: Biomacromolecules
https://www.readbyqxmd.com/read/27499575/f-fucoidan-from-saccharina-japonica-is-a-novel-inducer-of-galectin-9-and-exhibits-anti-allergic-activity
#18
Yuka Tanino, Takashi Hashimoto, Takao Ojima, Masashi Mizuno
Fucoidan is a sulfated polysaccharide from brown sea algae. In the present study, it was demonstrated that oral administration of F-fucoidan from Saccharina japonica possessed anti-allergic effects using the passive cutaneous anaphylaxis reaction, but not by intraperitoneal administration. The inhibitory mechanism was dependent on galectin-9, which belongs to a soluble lectin family that recognizes β-galactoside and prevents IgE binding to mast cells. The anti-allergy properties of F-fucoidan were cancelled by an intravenous dose of anti-galectin-9 antibody or lactose, which bind competitively with galectin-9 before the passive cutaneous anaphylaxis reaction...
July 2016: Journal of Clinical Biochemistry and Nutrition
https://www.readbyqxmd.com/read/27446078/the-effect-of-a-bsa-conjugate-of-a-synthetic-hexasaccharide-related-to-the-fragment-of-capsular-polysaccharide-of-streptococcus-pneumoniae-type-14-on-the-activation-of-innate-and-adaptive-immune-responses
#19
Nelli K Akhmatova, Ekaterina A Kurbatova, Elvin A Akhmatov, Nadezhda B Egorova, Denis Yu Logunov, Marina L Gening, Elena V Sukhova, Dmitry V Yashunsky, Yury E Tsvetkov, Nikolay E Nifantiev
We report the effect of a bovine serum albumin (BSA) conjugate of a synthetic hexasaccharide (HS) related to the fragment of the capsular polysaccharide (PS) of Streptococcus pneumoniae type 14 on the stimulation of innate immune system and the subsequent development of a PS-specific antibody response. Glycoconjugate (GC) in the presence (GC + AL) or absence of aluminum hydroxide was administered to mice twice. GC increased the number of TLR2-expressing cells and induced the maturation of dendritic cells (CD11c(+), CD80(+) and, MHCII(+)), which secreted IL-1β, IL-6, and TNFα into the culture medium...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27439378/novel-adjuvant-alum-tlr7-significantly-potentiates-immune-response-to-glycoconjugate-vaccines
#20
Cecilia Buonsanti, Cristiana Balocchi, Carole Harfouche, Federica Corrente, Luisa Galli Stampino, Francesca Mancini, Marta Tontini, Padma Malyala, Simone Bufali, Barbara Baudner, Ennio De Gregorio, Nicholas M Valiante, Derek T O'Hagan, Rino Rappuoli, Ugo D'Oro
Although glycoconjugate vaccines are generally very efficacious, there is still a need to improve their efficacy, especially in eliciting a strong primary antibody response. We have recently described a new type of vaccine adjuvant based on a TLR7 agonist adsorbed to alum (Alum-TLR7), which is highly efficacious at enhancing immunogenicity of protein based vaccines. Since no adjuvant has been shown to potentiate the immune response to glycoconjugate vaccines in humans, we investigated if Alum-TLR7 is able to improve immunogenicity of this class of vaccines...
2016: Scientific Reports
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