keyword
https://read.qxmd.com/read/38541162/seroprevalence-study-of-anti-hbs-antibodies-in-the-general-population-of-vojvodina-serbia
#1
JOURNAL ARTICLE
Smiljana Rajčević, Snežana Medić, Aleksandra Patić, Nataša Dragnić, Mioljub Ristić, Vladimir Vuković, Vladimir Petrović
Background and Objectives : Hepatitis B (HB) is a major global health problem and a potentially life-threatening disease caused by the hepatitis B virus (HBV). Also, it is an important cause of morbidity and mortality worldwide. Thanks to serological surveys, testing hepatitis B surface antibodies (anti-HBs) allows for serological assessments of their prevalence. The presence of anti-HBs, which protects against HBV infection, can be attributed to HB vaccination or natural HBV infection. The aim of our study was to evaluate the prevalence of HB surface antibodies (anti-HBs) as an indicator of collective immunity against HBV in the general population of the Autonomous Province of Vojvodina, Serbia...
March 6, 2024: Medicina
https://read.qxmd.com/read/38517269/the-seroepidemiology-of-immunoglobulin-g-antibodies-against-pertussis-toxin-and-filamentous-hemagglutinin-in-the-east-of-china-during-the-covid-19-pandemic
#2
JOURNAL ARTICLE
Xiang Sun, Tingting Zhang, Jinning Sun, Juan Zhou, Qiang Chen, Chengmei Jia, Yan Xu, Yun Wu, Zhiguo Wang, Wen Wang
This study employed sero-epidemiological methods to estimate the incidence of pertussis within a healthy population located in eastern China. The aim was to gain deeper insights into the epidemiological characteristics and burden of pertussis within the country. Blood samples were collected from healthy individuals in Jiangsu Province between June 2019 and December 2022. The levels of IgG antibodies against pertussis toxin (anti-PT) and filamentous hemagglutinin (anti-FHA) in the serum were quantitatively measured using enzyme-linked immunosorbent assay (ELISA)...
December 31, 2024: Human Vaccines & Immunotherapeutics
https://read.qxmd.com/read/38478521/detection-and-phylogenetic-analysis-of-kinetoplast-dna-of-leishmania-infantum-infected-humans-domestic-dogs-and-sandflies-in-northwest-iran
#3
JOURNAL ARTICLE
Hamed Behniafar, Niloofar Taghipour, Adel Spotin, Zabih Zare, Seyyed Javad Seyyed Tabaei, Elham Kazemirad, Vahideh Moin Vaziri, Mehdi Mohebali
Leishmaniasis refers to a disease with a wide range of manifestations; and there are three main forms of disease, cutaneous, mucocutaneous, and visceral. Leishmaniasis is one of the diseases with a protozoan agent which is vector-borne. Visceral leishmaniasis (VL) is the most severe form that can be fiercely life-threatening if left untreated. VL can be caused by members of Leishmania donovani complex, in Iran, Leishmania infantum is considered the primary causative agent of VL, resulting in a zoonotic form of VL...
2024: PloS One
https://read.qxmd.com/read/38456018/seroprevalence-of-crimean-congo-hemorrhagic-fever-virus-in-the-population-of-turkestan-region
#4
JOURNAL ARTICLE
Gulzhan Narkenovna Abuova, Farida Abdullayevna Berdaliyeva, Tatyana Vasiliyevna Polukchi, Daulet Sabyrovich Aliyev, Gulamzhan Sattarkulovich Raymkulov, Maksim Vladimirovich Kulemin, Ratbek Saylaubekuly
PURPOSE: To determine the level of seroprevalence to the virus of Crimean-Congo Hemorrhagic Fever (CCHF) in population of rural area of the particular districts of Turkestan region, Kazakhstan and its connection with epidemiological alerts. MATERIALS AND METHODS: For determining the real Crimean-Congo hemorrhagic fever virus (CCHFV) circulation in Turkestan region, Kazakhstan, massive sero-epidemiologic studies were conducted. Healthy residents of Turkestan Region aged from 10 to 75 years were examined without having been previously registered with CCHF...
2024: Le Infezioni in Medicina
https://read.qxmd.com/read/38447459/one-health-in-action-investigation-of-the-first-detected-local-cluster-of-fatal-borna-disease-virus-1-bodv-1-encephalitis-germany-2022
#5
JOURNAL ARTICLE
Merle M Böhmer, Viola C Haring, Barbara Schmidt, Franziska S Saller, Liza Coyer, Lidia Chitimia-Dobler, Gerhard Dobler, Dennis Tappe, Andrea Bonakdar, Arnt Ebinger, Gertrud Knoll, Lisa Eidenschink, Anette Rohrhofer, Hans Helmut Niller, Katharina Katz, Philip Starcky, Martin Beer, Rainer G Ulrich, Dennis Rubbenstroth, Markus Bauswein
BACKGROUND: Zoonotic Borna disease virus 1 (BoDV-1) causes fatal encephalitis in humans and animals. Subsequent to the detection of two paediatric cases in a Bavarian municipality in Germany within three years, we conducted an interdisciplinary One Health investigation. We aimed to explore seroprevalence in a local human population with a risk for BoDV-1 exposure as well as viral presence in environmental samples from local sites and BoDV-1 prevalence within the local small mammal population and its natural reservoir, the bicoloured white-toothed shrew (Crocidura leucodon)...
February 23, 2024: Journal of Clinical Virology
https://read.qxmd.com/read/38401400/development-of-an-elisa-for-the-detection-of-fowl-adenovirus-serotype-4-utilizing-fiber-protein
#6
JOURNAL ARTICLE
Faiza Ather, Muhammad Ashir Zia, Mudasser Habib, Muhammad Salah-Ud-Din Shah
Hydropericardium syndrome (HPS), caused by the Fowl adenovirus 4 (FAdV-4) has led to significant financial losses for the poultry industry globally, including Pakistan over the past few years. Conventional serological methods are time consuming, laborious and less sensitive therefore, a rapid and sensitive ELISA kit is required for the reliable detection of FAdV-4 infection. In the current research, fiber proteins (1 &2) of FAdV-4 were successfully expressed in Escherichia coli and purified using metal affinity chromatography...
February 23, 2024: Biologicals: Journal of the International Association of Biological Standardization
https://read.qxmd.com/read/38389083/sero-epidemiological-investigation-on-toxoplasma-gondii-infection-in-apennine-wolf-canis-lupus-italicus-and-wild-boar-sus-scrofa-in-italy
#7
JOURNAL ARTICLE
Filippo Maria Dini, Carmela Musto, Vincenzo Maria De Nigris, Enrica Bellinello, Maria Sampieri, Giuseppe Merialdi, Lorella Barca, Mauro Delogu, Roberta Galuppi
BACKGROUND: The wild boar (Sus scrofa) and the Apennine wolf (Canis lupus italicus) are two wild species that have both increased their presence in the Italian territory, albeit in varying numbers. They can be occasionally found in peri-urban areas as well. Both of these species can serve as intermediate hosts for Toxoplasma gondii, as they can become infected either through the consumption of oocysts found in water, soil, or on vegetables, or through the ingestion of meat containing bradyzoites...
February 22, 2024: BMC Veterinary Research
https://read.qxmd.com/read/38329335/epidemiological-clinical-and-laboratory-characteristics-of-human-granulocytic-anaplasmosis-in-north-india
#8
JOURNAL ARTICLE
E V Vinayaraj, Chandan Kumar Thakur, Preeti Negi, K Sreenath, Priyanka Upadhyay, Nishant Verma, Bimal Kumar Das, S K Kabra, Naveet Wig, Rama Chaudhry
Human granulocytic anaplasmosis (HGA) is an emerging, rickettsial tick-borne disease caused by Anaplasma phagocytophilum . Sero-epidemiological data demonstrate that this pathogen has a worldwide distribution. The diagnosis of HGA requires a high index of clinical suspicion, even in endemic areas. In recent years, HGA has increasingly been reported from Asia and described in China, Japan, and Korea. We serologically and molecularly screened 467 patients with clinical suspicion of Anaplasmosis. The present study describes the epidemiology, clinical, and laboratory details of 6 confirmed and 43 probable cases of human granulocytic anaplasmosis...
February 8, 2024: Journal of Clinical Microbiology
https://read.qxmd.com/read/38237697/development-and-characterization-of-a-hemolysis-inhibition-assay-to-determine-functionality-of-anti-streptolysin-o-antibodies-in-human-sera
#9
JOURNAL ARTICLE
Martina Carducci, Alana Whitcombe, Luca Rovetini, Luisa Massai, Alexander J Keeley, Thushan I de Silva, Julie Bennett, Francesco Berlanda Scorza, Miren Iturriza, Nicole J Moreland, Danilo G Moriel, Omar Rossi
The high burden of disease and the long-lasting sequelae following Streptococcus pyogenes (Strep A) infections make the development of an effective vaccine a global health priority. Streptolysin O (SLO), is a key toxin in the complex pathogenesis of Strep A infection. Antibodies are elicited against SLO after natural exposure and represent a key target for vaccine-induced immunity. Here we present the setup and characterization of a hemolysis assay to measure functionality of anti-SLO antibodies in human sera...
January 16, 2024: Journal of Immunological Methods
https://read.qxmd.com/read/38195403/identification-of-associated-risk-factors-for-serological-distribution-of-hepatitis-b-virus-via-machine-learning-models
#10
JOURNAL ARTICLE
Ning Yao, Yang Liu, Jiawei Xu, Qing Wang, Quanhua Zhou, Yue Wang, Dong Yi, Yazhou Wu
BACKGROUND: The provincial-level sero-survey was launched to learn the updated seroprevalence of hepatitis B virus (HBV) infection in the general population aged 1-69 years in Chongqing and to assess the risk factors for HBV infection to effectively screen persons with chronic hepatitis B (CHB). METHODS: A total of 1828 individuals aged 1-69 years were investigated, and hepatitis B surface antigen (HBsAg), antibody to HBsAg (HBsAb), and antibody to B core antigen (HBcAb) were detected...
January 9, 2024: BMC Infectious Diseases
https://read.qxmd.com/read/38156306/persistence-of-sars-cov-2-antibodies-against-n-s-and-rbd-after-natural-infection
#11
JOURNAL ARTICLE
Mitra Jafari, Samira Asli, Fateme Moghbeli, Mohammad Gholami Fesharaki, Nazila Hajiahmadi, Faezeh Mojtahedzadeh, Saeid Amel Jamehdar, Taravat Bamdad
BACKGROUND AND OBJECTIVES: Coronavirus disease 2019 (COVID-19) pandemic has affected most countries in the world. Monitoring the humoral immune responses during the natural course of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection and the duration of them provide useful information for the development of vaccination strategies against this virus and its emerging variants. The importance of the antibody response especially neutralizing antibodies in long-term immunity to SARS-CoV-2 is significant...
December 2023: Iranian Journal of Microbiology
https://read.qxmd.com/read/38118336/equine-influenza-outbreak-in-eastern-of-algeria-in-2021-the-first-introduction-of-florida-clade-1-to-maghreb-area
#12
JOURNAL ARTICLE
Hadda Kareche, José-Carlos Valle-Casuso, Anthony Madeline, Delphine Froger, Fanny Lecouturier, Gaëlle Gonzalez, Abdelaali Debbi, Saber Taqiyeddine Benseghir, Abdelkrim Mohamed Nasri, Moundher Boureghda, Abdelhamid Achouri, Farouk Laabassi
We have performed an equine influenza (EI) serological study of the equine population in Algeria by testing 298 serum samples collected between February and August 2021 from 5 provinces. The results were obtained performing an NP-ELISA. Our results revealed that 49.3% (147/298) samples positive for antibodies to EI (H3N8). During this study and after a gap of one decade an outbreak of EI was reported in Algeria in the first week of March 2021. The disease was confirmed by virus detection from the nasal swabs (n = 39) by qRT-PCR and by identifying 5 EI seroconversion...
December 4, 2023: Comparative Immunology, Microbiology and Infectious Diseases
https://read.qxmd.com/read/38106811/cell-mediated-immunity-cmi-for-sars-cov-2-infection-among-the-general-population-of-north-india-a-cross-sectional-analysis-from-a-sub-sample-of-a-large-sero-epidemiological-study
#13
JOURNAL ARTICLE
Puneet Misra, Pramod K Garg, Amit Awasthi, Shashi Kant, Sanjay K Rai, Mohammad Ahmad, Randeep Guleria, Trideep J Deori, Suprakash Mandal, Abhishek Jaiswal, Gaurav Gongal, Siddhesh Vishwakarma, Mohan Bairwa, Rakesh Kumar, Partha Haldar, Akshay Binayke
Background Cell-mediated immunity (CMI), or specifically T-cell-mediated immunity, is proven to remain largely preserved against the variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including Omicron. The persistence of cell-mediated immune response in individuals longitudinally followed up for an extended period remains largely unelucidated. To address this, the current study was planned to study whether the effect of cell-mediated immunity persists after an extended period of convalescence or vaccination...
November 2023: Curēus
https://read.qxmd.com/read/38099622/improved-diagnostic-sensitivity-of-human-strongyloidiasis-using-point-of-care-mixed-recombinant-antigen-based-immunochromatography
#14
JOURNAL ARTICLE
Patcharaporn Boonroumkaew, Lakkhana Sadaow, Penchom Janwan, Rutchanee Rodpai, Oranuch Sanpool, Punyisa Buadee, Chanida Suprom, Tongjit Thanchomnang, Pewpan M Intapan, Wanchai Maleewong
Strongyloidiasis is a neglected tropical disease that can cause fatal complications due to hyperinfection and disseminated strongyloidiasis in immunocompromised patients. We used two Strongyloides stercoralis recombinant antigenic proteins, L3NieAg.01 (NIE) and IgG-immunoreactive antigen (SsIR), to develop the recombinant antigen-based immunochromatography test (ICT) kit. We constructed and compared kits using either the NIE (NIE ICT kit) or the SsIR (SsIR ICT kit) antigens and a kit using a mixture of both (NIE-SsIR ICT kit) for detection of anti-Strongyloides IgG antibody in human serum samples...
2023: Parasite: Journal de la Société Française de Parasitologie
https://read.qxmd.com/read/38079384/anti-sars-cov-2-antibody-kinetics-up-to-6-months-of-follow-up-result-from-a-nation-wide-population-based-age-stratified-sero-epidemiological-prospective-cohort-study-in-india
#15
JOURNAL ARTICLE
Puneet Misra, Arvind Kumar Singh, Baijayantimala Mishra, Bijayini Behera, Binod Kumar Patro, Guruprasad R Medigeshi, Hari Shanker Joshi, Mohammad Ahmad, Pradeep Kumar Chaturvedi, Palanivel Chinnakali, Partha Haldar, Mohan Bairwa, Pradeep Kharya, Rahul Dhodapkar, Ramashankar Rath, Randeep Guleria, Sanjay Kumar Rai, Sitanshu Sekhar Kar, Shashi Kant, Sonali Sarkar, Subrata Baidya, Suneeta Meena, Suprakash Mandal, Surekha Kishore, Tapan Majumder, Vivek Hada
Repeated serological testing tells about the change in the overall infection in a community. This study aimed to evaluate changes in antibody prevalence and kinetics in a closed cohort over six months in different sub-populations in India. The study included 10,000 participants from rural and urban areas in five states and measured SARS-CoV-2 antibodies in serum in three follow-up rounds. The overall seroprevalence increased from 73.9% in round one to 90.7% in round two and 92.9% in round three. Among seropositive rural participants in round one, 98...
2023: PloS One
https://read.qxmd.com/read/38070129/seroepidemiology-of-hbv-antigen-and-anti-hcv-among-general-population-in-a-rural-local-in-bauchi-state-nigeria
#16
M Alkali, K O Okon, M S Umar, M D Sani, H Shuaibu, R S Babale, M Sani, G H Mohammed, B Tar, L Amadu
BACKGROUND: HBV and HCV infections are a significant public health issue in developing countries with weak healthcare systems, high poverty rates, illiteracy, low HBV immunization coverage, and low public health education. A study assessed the sero epidemiology of HBV antigen, anti- HCV markers, biochemical and heamatological indices of 559 participants in Dambam local government during hepatitis day. A structured questionnaire was administered to assess demographic information and risk factors...
December 4, 2023: West African Journal of Medicine
https://read.qxmd.com/read/38019697/the-burden-of-the-coronavirus-disease-2019-virus-infection-in-burkina-faso-results-from-a-world-health-organization-unity-population-based-age-stratified-sero-epidemiological-investigation
#17
JOURNAL ARTICLE
Samiratou Ouedraogo, Isidore Tiandiogo Traoré, Dramane Kania, Nongodo Firmin Kaboré, Ariane Mamguem Kamga, Hermann Badolo, Mimbouré Yara, Guillaume Sanou, Amariane Koné, Samdapawindé Thérèse Kagoné, Esperance Ouédraogo, Blahima Konaté, Rachel Médah, Nathalie de Rekeneire, Armel Poda, Arnaud Eric Diendere, Boukary Ouédraogo, Oumar Billa, Gilles Paradis, Halidou Tinto, Tienhan Sandrine Dabakuyo-Yonli
BACKGROUND: This study aimed to estimate the anti-SARS-CoV-2 antibody seroprevalence in the general population of Bobo-Dioulasso and Ouagadougou (Burkina Faso). METHODS: We collected from March to April 2021 blood samples from randomly selected residents in both main cities based on the World Health Organization (WHO) sero-epidemiological investigations protocols and tested them with WANTAI SARS-CoV-2 total antibodies enzyme-linked immunosorbent assay (ELISA) kits intended for qualitative assessment...
November 2023: Influenza and Other Respiratory Viruses
https://read.qxmd.com/read/38007988/serological-and-molecular-survey-of-brucellosis-and-chlamydiosis-in-dromedary-camels-from-tunisia
#18
JOURNAL ARTICLE
Rachid Selmi, Aymen Mamlouk, Hanene Belkahia, Houcine Ben Yahia, Hedi Abdelaali, Mohamed-Habib Jemli, Mourad Ben Said, Lilia Messadi
The present sero-epidemiological survey was designed and conducted to scrutinize the current status of camel-related brucellosis and chlamydiosis in Tunisia. Whole blood and serum samples were collected from 470 dromedaries (Camelus dromedarius) from eight different Tunisian governorates. Serum samples were subjected to indirect enzyme-linked immunosorbent assay (iELISA). The detection of Brucella and Chlamydia DNA was performed using conventional PCR targeting the bcsp-31 and 16 S rRNA gene, respectively...
November 15, 2023: Comparative Immunology, Microbiology and Infectious Diseases
https://read.qxmd.com/read/37999612/using-regional-sero-epidemiology-sars-cov-2-anti-s-antibodies-in-the-dominican-republic-to-inform-targeted-public-health-response
#19
JOURNAL ARTICLE
Beatris Mario Martin, Angela Cadavid Restrepo, Helen J Mayfield, Cecilia Then Paulino, Micheal De St Aubin, William Duke, Petr Jarolim, Emily Zielinski Gutiérrez, Ronald Skewes Ramm, Devan Dumas, Salome Garnier, Marie Caroline Etienne, Farah Peña, Gabriela Abdalla, Beatriz Lopez, Lucia de la Cruz, Bernarda Henríquez, Margaret Baldwin, Benn Sartorius, Adam Kucharski, Eric James Nilles, Colleen L Lau
Incidence of COVID-19 has been associated with sociodemographic factors. We investigated variations in SARS-CoV-2 seroprevalence at sub-national levels in the Dominican Republic and assessed potential factors influencing variation in regional-level seroprevalence. Data were collected in a three-stage cross-sectional national serosurvey from June to October 2021. Seroprevalence of antibodies against the SARS-CoV-2 spike protein (anti-S) was estimated and adjusted for selection probability, age, and sex. Multilevel logistic regression was used to estimate the effect of covariates on seropositivity for anti-S and correlates of 80% protection (PT80 ) against symptomatic infection for the ancestral and Delta strains...
November 4, 2023: Tropical Medicine and Infectious Disease
https://read.qxmd.com/read/37896799/severe-neurologic-disease-in-a-horse-caused-by-tick-borne-encephalitis-virus-austria-2021
#20
JOURNAL ARTICLE
Phebe de Heus, Zoltán Bagó, Pia Weidinger, Dilara Lale, Dagmar S Trachsel, Sandra Revilla-Fernández, Kaspar Matiasek, Norbert Nowotny
As evidenced by sero-epidemiological studies, infections of horses with the tick-borne encephalitis virus (TBEV) occur frequently in TBEV-endemic areas. However, there are only very few reports of clinical cases. A possible underreporting may be due to a variety of diagnostic challenges. In this study, ELISA and neutralization tests were applied to serum samples. Brain tissue samples were investigated for the presence of nucleic acids of TBEV, Equid alphaherpesvirus 1 , Borna disease virus 1, West Nile and Usutu viruses, rustrela virus, as well as Eastern, Western, and Venezuelan equine encephalitis viruses with RT-qPCR, RT-PCR, and qPCR, respectively...
September 29, 2023: Viruses
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