Evelyne Zeira, Alexandra Manevitch, Zakharia Manevitch, Eli Kedar, Michal Gropp, Nili Daudi, Rimma Barsuk, Menahem Harati, Hagit Yotvat, Philip J Troilo, Thomas G Griffiths, Stephen J Pacchione, Dana F Roden, Zhutian Niu, Ofer Nussbaum, Gideon Zamir, Orit Papo, Izhack Hemo, Aaron Lewis, Eithan Galun
A femtosecond laser beam gene transduction (SG-LBGT) system is described as a novel and efficient method of intradermal (i.d.) nonviral gene delivery in mice by permeabilizing cells utilizing femtosecond laser pulses. Using this approach, significant gene expression and efficient dermal transduction lasting for >7 months were obtained. The ability of this new DNA gene transfer method to enhance genetic vaccination was tested in BALB/C mice. A single i.d. injection of a plasmid (10 microg) containing the hepatitis B virus (HBV) surface antigen (HBsAg), followed by pulses of laser, induced high titers of HBsAg-specific antibodies lasting for >210 days and increased levels of IgG1, IgG2a, IFNgamma, and IL-4, indicating the activation of both Th1 and Th2 cells...
November 2007: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology