keyword
MENU ▼
Read by QxMD icon Read
search

Elegans stress

keyword
https://www.readbyqxmd.com/read/29770276/the-evaluation-of-anoxia-responsive-e2f-dna-binding-activity-in-the-red-eared-slider-turtle-trachemys-scripta-elegans
#1
Kyle K Biggar, Kenneth B Storey
In many cases, the DNA-binding activity of a transcription factor does not change, while its transcriptional activity is greatly influenced by the make-up of bound proteins. In this study, we assessed the protein composition and DNA-binding ability of the E2F transcription factor complex to provide insight into cell cycle control in an anoxia tolerant turtle through the use of a modified ELISA protocol. This modification also permits the use of custom DNA probes that are tailored to a specific DNA binding region, introducing the ability to design capture probes for non-model organisms...
2018: PeerJ
https://www.readbyqxmd.com/read/29769649/organoruthenium-ii-complexes-ameliorates-oxidative-stress-and-impedes-the-age-associated-deterioration-in-caenorhabditis-elegans-through-jnk-1-daf-16-signalling
#2
G Devagi, A Mohankumar, G Shanmugam, S Nivitha, F Dallemer, P Kalaivani, P Sundararaj, R Prabhakaran
New ruthenium(II) complexes were synthesised and characterized by various spectro analytical techniques. The structure of the complexes 3 and 4 has been confirmed by X-ray crystallography. The complexes were subjected to study their anti-oxidant profile and were exhibited significantly greater in vitro DPPH radical scavenging activity than vitamin C. We found that complexes 1-4 confered tolerance to oxidative stress and extend the mean lifespan of mev-1 mutant worms and wild-type Caenorhabditis elegans. Further, mechanistic study and reporter gene expression analysis revealed that Ru(ƞ6 -p-cymene) complexes maintained the intracellular redox status and offers stress resistance through activating JNK-1/DAF-16 signaling axis and possibly by other antioxidant response pathway...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29766797/anti-aging-and-anti-aggregation-properties-of-polyphenolic-compounds-in-c-elegans
#3
Nikoletta Papaevgeniou, Niki Chondrogianni
Polyphenols constitute a group of compounds that have been highly investigated for their beneficial effects against various pathologic and non-pathologic conditions and diseases. Among their multi-faceted properties, their anti-oxidant potential nominates them as ideal protective candidates for conditions characterized by elevated levels of oxidative stress, including aging and age-related diseases. The nematode Caenorhabditis elegans is a multicellular model organism that is highly exploited in studies related to aging and age-associated diseases...
May 15, 2018: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/29752286/genetic-and-cellular-sensitivity-of-caenorhabditis-elegans-to-the-chemotherapeutic-agent-cisplatin
#4
Francisco Javier García-Rodríguez, Carmen Martínez-Fernández, David Brena, Dmytro Kukhtar, Xènia Serrat, Ernest Nadal, Mike Boxem, Sebastian Honnen, Antonio Miranda-Vizuete, Alberto Villanueva, Julián Cerón
Cisplatin and derivatives are commonly used as chemotherapeutic agents. Although the cytotoxic action of cisplatin on cancer cells is very efficient, clinical oncologists need to deal with two major difficulties: (i) the onset of resistance to the drug, and (ii) the cytotoxic effect in patients. Here we use Caenorhabditis elegans to investigate factors influencing the response to cisplatin in multicellular organisms. In this hermaphroditic model organism, we observed that sperm failure is a major cause in cisplatin-induced infertility...
May 10, 2018: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/29748634/gene-by-environment-interactions-that-disrupt-mitochondrial-homeostasis-cause-neurodegeneration-in-c-elegans-parkinson-s-models
#5
Hanna Kim, Rylee J Perentis, Guy A Caldwell, Kim A Caldwell
Parkinson's disease (PD) is a complex multifactorial disorder where environmental factors interact with genetic susceptibility. Accumulating evidence suggests that mitochondria have a central role in the progression of neurodegeneration in sporadic and/or genetic forms of PD. We previously reported that exposure to a secondary metabolite from the soil bacterium, Streptomyces venezuelae, results in age- and dose-dependent dopaminergic (DA) neurodegeneration in Caenorhabditis elegans and human SH-SY5Y neurons...
May 10, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29746159/caenorhabditis-elegans-tolerates-hyperaccelerations-up-to-400-000-x-g
#6
Tiago Alves Jorge de Souza, Tiago Campos Pereira
One of the most important laboratory animal species is the nematode Caenorhabditis elegans, which has been used in a range of research fields such as neurobiology, body development, and molecular biology. The scientific progress obtained by employing C. elegans as a model in these areas has encouraged its use in new fields. One of the new potential applications concerns the biological responses to hyperacceleration stress (g-force), but only a few studies have evaluated the response of multicellular organisms to extreme hypergravity conditions at the order of magnitude 105  x g, which is the theorized force experienced by rocks ejected from Mars (or similar planets)...
May 10, 2018: Astrobiology
https://www.readbyqxmd.com/read/29743244/pore-lining-residues-of-mec-4-and-mec-10-channel-subunits-tune-the-caenorhabditis-elegans-degenerin-channel-s-response-to-shear-stress
#7
Shujie Shi, Stephanie M Mutchler, Brandon M Blobner, Ossama B Kashlan, Thomas R Kleyman
The Caenorhabditis elegans MEC-4/MEC-10 channel mediates the worm's response to gentle body touch and is activated by laminar shear stress (LSS) when expressed in Xenopus oocytes. Substitutions   at multiples sites within the second transmembrane helix (TM2) of MEC-4 or MEC-10 abolish the gentle touch response in worms, but the roles of these residues in mechanosensing are unclear. The present study therefore examined the role of specific MEC-4 and MEC-10 TM2 residues in the channel's response to LSS. We found that introducing mutations within the TM2 of MEC-4 or MEC-10 not only altered channel activity, but also affected the channel's response to LSS...
May 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29740339/induction-of-metallothionein-expression-after-exposure-to-conventional-cigarette-smoke-but-not-electronic-cigarette-ecig-generated-aerosol-in-caenorhabditis-elegans
#8
Eric Cobb, Julie Hall, Dominic L Palazzolo
Aim: With the invention of electronic cigarettes (ECIG), many questions have been raised regarding their safety as an alternative to smoking conventional cigarettes. Conventional cigarette smoke contains a variety of toxicants including heavy metals. However, ECIG-generated aerosol contains only trace amounts of metals, adding to the argument for it being a safer alternative. In response to heavy metal exposure, metallothioneins are induced in cells to help store the metal, detoxify the body, and are also known responders to oxidative stress...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29723292/caenorhabditis-elegans-susceptibility-to-daldinia-cf-concentrica-bioactive-volatiles-is-coupled-with-expression-activation-of-the-stress-response-transcription-factor-daf-16-a-part-of-distinct-nematicidal-action
#9
Payal Sanadhya, Patricia Bucki, Orna Liarzi, David Ezra, Abraham Gamliel, Sigal Braun Miyara
The bionematicidal effect of a synthetic volatile mixture (SVM) of four volatile organic compounds (VOCs) emitted by the endophytic fungus Daldinia cf. concentrica against the devastating plant-parasitic root-knot nematode Meloidogyne javanica has been recently demonstrated in both in vitro and greenhouse experiments. However, the mode of action governing the observed irreversible paralysis of J2 larvae upon exposure to SVM is unknown. To unravel the mechanism underlying the anthelmintic and nematicidal activities, we used the tractable model worm Caenorhabditis elegans...
2018: PloS One
https://www.readbyqxmd.com/read/29714684/set-9-and-set-26-are-h3k4me3-readers-and-play-critical-roles-in-germline-development-and-longevity
#10
Wenke Wang, Amaresh Chaturbedi, Minghui Wang, Serim An, Satheeja Santhi, Siu Sylvia Lee
C. elegans SET-9 and SET-26 are highly homologous paralogs that share redundant functions in germline development, but SET-26 alone plays a key role in longevity and heat stress response. SET-26 is broadly expressed, but SET-9 is only detectable in the germline, which likely account for their different biological roles. SET-9 and SET-26 bind to H3K4me3 with adjacent acetylation marks in vitro and in vivo . In the soma, SET-26 acts through DAF-16 to modulate longevity. In the germline, SET-9 and SET-26 restrict H3K4me3 domains around SET-9 and SET-26 binding sites, and regulate the expression of specific target genes, with critical consequence on germline development...
May 1, 2018: ELife
https://www.readbyqxmd.com/read/29709515/5-methoxyindole-2-carboxylic-acid-mica-suppresses-a%C3%AE-mediated-pathology-in-c-elegans
#11
Waqar Ahmad, Paul R Ebert
Alzheimer's disease (AD) is an age-related disease characterized by loss of memory and disrupted thinking that is associated with altered energy metabolism. Variants of an important enzyme of energy metabolism, dihydrolipoamide dehydrogenase (dld), have been genetically linked to late-onset AD. Moreover, reduced activity of DLD-containing enzyme complexes is associated with AD progression. To understand how energy metabolism influences AD progression, we exposed C. elegans expressing human Aβ peptide to the chemical inhibitor of DLD, 2-methoxyindole-5-carboxylic acid (MICA)...
April 27, 2018: Experimental Gerontology
https://www.readbyqxmd.com/read/29707847/signatures-of-local-adaptation-along-environmental-gradients-in-a-range-expanding-damselfly-ischnura-elegans
#12
Rachael Y Dudaniec, Chuan Ji Yong, Lesley T Lancaster, Erik I Svensson, Bengt Hansson
Insect distributions are shifting rapidly in response to climate change and are undergoing rapid evolutionary change. We investigate the molecular signatures underlying local adaptation in the range-expanding damselfly, Ischnura elegans. Using a landscape genomic approach combined with generalized dissimilarity modelling (GDM), we detect selection signatures on loci via allelic frequency change along environmental gradients. We analyse 13,612 Single Nucleotide Polymorphisms (SNPs), derived from Restriction site-Associated DNA sequencing (RADseq), in 426 individuals from 25 sites spanning the I...
April 29, 2018: Molecular Ecology
https://www.readbyqxmd.com/read/29707606/measurement-of-ros-in-caenorhabditis-elegans-using-a-reduced-form-of-fluorescein
#13
Shaarika Sarasija, Kenneth R Norman
Oxidative stress is implicated in the pathogenesis of various neurodegenerative diseases, including Alzheimer's disease. Oxidative stress is a result of a disruption of the equilibrium between antioxidants and oxidants, in favor of oxidants. Since mitochondria are major sites of production and reduction of reactive oxygen species (ROS), measurement of ROS levels can help us determine if mitochondrial functional integrity has been compromised. In this protocol, we describe a method to measure the level of ROS in the nematode Caenorhabditis elegans, using chloromethyl-2,7'-dichlorodihydrofluorescein diacetate (CM-H2 DCFDA)...
April 5, 2018: Bio-protocol
https://www.readbyqxmd.com/read/29703325/constituent-and-effects-of-polysaccharides-isolated-from-sophora-moorcroftiana-seeds-on-lifespan-reproduction-stress-resistance-and-antimicrobial-capacity-in-caenorhabditis-elegans
#14
Yuan Zhang, Dan-Yang Mi, Jin Wang, Yan-Ping Luo, Xu Yang, Shi Dong, Xing-Ming Ma, Kai-Zhong Dong
Sophora moorcroftiana (S. moorcroftiana) is an endemic leguminous dwarf shrub in Tibet, China. Decoctions of the seeds have been used in Chinese folk medicine for dephlogistication, detoxication, and infectious diseases. The present study aimed to investigate the constituent and biological effects of polysaccharides from S. moorcroftiana seeds in Caenorhabditis elegans (C. elegans). Polysaccharides from S. moorcroftiana seeds (SMpol) were extracted with 60% ethanol and constituent was analyzed by GC-MS. SMpol was composed of glucose, galactose and inositol in the molar ratio of 35...
April 2018: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/29672544/stress-pre-conditioning-with-temperature-uv-and-gamma-radiation-induces-tolerance-against-phosphine-toxicity
#15
Saad M Alzahrani, Paul R Ebert
Phosphine is the only general use fumigant for the protection of stored grain, though its long-term utility is threatened by the emergence of highly phosphine-resistant pests. Given this precarious situation, it is essential to identify factors, such as stress preconditioning, that interfere with the efficacy of phosphine fumigation. We used Caenorhabditis elegans as a model organism to test the effect of pre-exposure to heat and cold shock, UV and gamma irradiation on phosphine potency. Heat shock significantly increased tolerance to phosphine by 3-fold in wild-type nematodes, a process that was dependent on the master regulator of the heat shock response, HSF-1...
2018: PloS One
https://www.readbyqxmd.com/read/29670680/gengnianchun-extends-the-lifespan-of-caenorhabditis-elegans-via-the-insulin-igf-1-signalling-pathway
#16
Fanhui Meng, Jun Li, Yanqiu Rao, Wenjun Wang, Yan Fu
Gengnianchun (GNC), a traditional Chinese medicine (TCM), is believed to have beneficial effects on ageing-related diseases, such as antioxidant properties and effects against A β -induced toxicity. We previously found that GNC extended the lifespan of Caenorhabditis elegans . However, the mechanism underlying this effect was unclear. In this study, we further explored the mechanisms of GNC using a C. elegans model. GNC significantly increased the lifespan of C. elegans and enhanced oxidative and thermal stress resistance...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29666286/viable-but-nonculturable-listeria-monocytogenes-and-salmonella-enterica-serovar-thompson-induced-by-chlorine-stress-remain-infectious
#17
Callum J Highmore, Jennifer C Warner, Steve D Rothwell, Sandra A Wilks, C William Keevil
The microbiological safety of fresh produce is monitored almost exclusively by culture-based detection methods. However, bacterial food-borne pathogens are known to enter a viable-but-nonculturable (VBNC) state in response to environmental stresses such as chlorine, which is commonly used for fresh produce decontamination. Here, complete VBNC induction of green fluorescent protein-tagged Listeria monocytogenes and Salmonella enterica serovar Thompson was achieved by exposure to 12 and 3 ppm chlorine, respectively...
April 17, 2018: MBio
https://www.readbyqxmd.com/read/29664006/a-genetic-program-mediates-cold-warming-response-and-promotes-stress-induced-phenoptosis-in-c-elegans
#18
Wei Jiang, Yuehua Wei, Yong Long, Arthur Owen, Bingying Wang, Xuebing Wu, Shuo Luo, Yongjun Dang, Dengke K Ma
How multicellular organisms respond to and are impacted by severe hypothermic stress is largely unknown. From C. elegans screens for mutants abnormally responding to cold-warming stimuli, we identify a molecular genetic pathway comprising ISY-1, a conserved uncharacterized protein, and ZIP-10, a bZIP-type transcription factor. ISY-1 gatekeeps the ZIP-10 transcriptional program by regulating the microRNA mir-60 . Downstream of ISY-1 and mir-60 , zip-10 levels rapidly and specifically increase upon transient cold-warming exposure...
April 17, 2018: ELife
https://www.readbyqxmd.com/read/29656019/proteomic-identification-of-virulence-related-factors-in-young-and-aging-c-elegans-infected-with-pseudomonas-aeruginosa
#19
Christina D King, Daljeet Singh, Kyle Holden, Annie B Govan, Scott A Keith, Arjumand Ghazi, Renã A S Robinson
The molecular mechanisms that distinguish immunosenescence from general age-related decline are poorly understood. We addressed this by exposing Day 1 and Day 5 adults of Caenorhabditis elegans to Pseudomonas aeruginosa strain PA01, an opportunistic pathogen. Day 5 adult C. elegans exhibited greater vulnerability to infection as compared to Day 1 C. elegans. Using TMT6 -plex isobaric labeling and reductive dimethylation, we identified 55 proteins whose levels were altered following infection of Day 1 and Day 5 adults...
April 12, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29622768/role-of-phospholipase-d-in-the-lifespan-of-caenorhabditis-elegans
#20
Jeong-Hwan Park, Jeong-Woo Park, Ju-Hyeon Lee, Dong-Yun Kim, Jeong-Hoon Hahm, Young-Seuk Bae
We have previously shown that phospholipase D (PLD) downregulation accelerates cellular senescence, which is widely believed to play an important role in aging, by stimulating reactive oxygen species (ROS) accumulation in human cells. In this study, we examined the role of PLD in aging using the nematode Caenorhabditis elegans. The mRNA level of pld-1 was found to be inversely correlated with aging. RNAi-mediated knockdown of pld-1 expression in nematodes enhanced ROS and lipofuscin accumulation and decreased lifespan, motility, and resistance to stress compared to that in nematodes treated with control RNAi...
April 6, 2018: Experimental & Molecular Medicine
keyword
keyword
52124
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"