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https://www.readbyqxmd.com/read/29774473/genomic-and-probiotic-characterization-of-sjp-snu-strain-of-pichia-kudriavzevii
#1
Seung-Min Hong, Hyuk-Joon Kwon, Se-Joon Park, Won-Jin Seong, Ilhwan Kim, Jae-Hong Kim
The yeast strain SJP-SNU was investigated as a probiotic and was characterized with respect to growth temperature, bile salt resistance, hydrogen sulfide reducing activity, intestinal survival ability and chicken embryo pathogenicity. In addition, we determined the complete genomic and mitochondrial sequences of SJP-SNU and conducted comparative genomics analyses. SJP-SNU grew rapidly at 37 °C and formed colonies on MacConkey agar containing bile salt. SJP-SNU reduced hydrogen sulfide produced by Salmonella serotype Enteritidis and, after being fed to 4-week-old chickens, could be isolated from cecal feces...
May 17, 2018: AMB Express
https://www.readbyqxmd.com/read/29763937/bacteriophage-cocktail-for-biocontrol-of-escherichia-coli-o157-h7-stability-and-potential-allergenicity-study
#2
Karina Ramirez, Carmina Cazarez-Montoya, Hector Samuel Lopez-Moreno, Nohelia Castro-Del Campo
Escherichia coli O157:H7 has become a global public health and a food safety problem. Despite the implementation of control strategies that guarantee the safety in various products, outbreaks persist and new alternatives are necessary to reduce this pathogen along the food chain. Recently, our group isolated and characterised lytic bacteriophages against E. coli O157:H7 with potential to be used as biocontrol agents in food. To this end, phages need certain requirements to allow their manufacture and application...
2018: PloS One
https://www.readbyqxmd.com/read/29762784/identification-and-characterization-of-probiotic-yeast-isolated-from-digestive-tract-of-ducks
#3
X Q Hu, Q Liu, J P Hu, J J Zhou, X Zhang, S Y Peng, L J Peng, X D Wang
The objective of this study was to isolate and identify yeast strains from the digestive tract of ducks, and evaluate in vitro their potential as probiotics in poultry. The yeast strains were isolated using malt extract agar medium, and identified through morphological, physiological, and biochemical tests as well as sequence homology analyses of 26S rDNA D1/D2 region. A total of 35 yeast strains were isolated from the guts of Cherry Valley meat ducks, including seven strains of Saccharomyces cerevisiae (S...
May 14, 2018: Poultry Science
https://www.readbyqxmd.com/read/29762617/anticancer-potential-against-cervix-cancer-hela-cell-line-of-probiotic-lactobacillus-casei-and-lactobacillus-paracasei-strains-isolated-from-human-breast-milk
#4
Muhammad Shahid Riaz Rajoka, Haobin Zhao, Yao Lu, Ziyang Lian, Na Li, Nazim Hussain, Dongyan Shao, Mingliang Jin, Qi Li, Junling Shi
Lactic acid bacteria have been categorized as probiotics and play a crucial role in human health by stimulating the supply of nutrients, shaping the immune system, and preventing the colonization of pathogenic microbes. This study investigated the mechanisms for the action of three potential probiotic Lactobacillus strains: Lactobacillus casei SR1, Lactobacillus casei SR2, and Lactobacillus paracasei SR4 isolated from human breast milk. These Lactobacillus strains were identified via 16S DNA sequencing and characterized via biochemical assays including acid resistance, bile resistance, antioxidant activity, and antibiotic susceptibility...
May 15, 2018: Food & Function
https://www.readbyqxmd.com/read/29762491/study-of-factors-influencing-the-bioaccessibility-of-triazolone-in-cherry-tomatoes-using-a-static-shime-model
#5
Yu-Ying Liu, Jin-Jing Xiao, Yun-Yao Fu, Min Liao, Hai-Qun Cao, Yan-Hong Shi
Estimating the influence of bioaccessibility of pesticide residues in fruits and vegetables on dietary exposure is a challenge for human health risk assessment. This study investigated the bioaccessibility of pesticide residues in cherry tomatoes and contributing factors (digestion time, pH, solid/liquid ratio, and dietary nutrition) using an in vitro test simulating the human gastrointestinal tract. pH had the largest effect on triazolone precipitation in the simulated gastric intestinal juice, which had a significant impact on the bioaccessibility...
May 15, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29761207/omics-based-responses-induced-by-bosentan-in-human-hepatoma-heparg-cell-cultures
#6
Robim M Rodrigues, Laxmikanth Kollipara, Umesh Chaudhari, Agapios Sachinidis, René P Zahedi, Albert Sickmann, Annette Kopp-Schneider, Xiaoqi Jiang, Hector Keun, Jan Hengstler, Marlies Oorts, Pieter Annaert, Eef Hoeben, Eva Gijbels, Joery De Kock, Tamara Vanhaecke, Vera Rogiers, Mathieu Vinken
Bosentan is well known to induce cholestatic liver toxicity in humans. The present study was set up to characterize the hepatotoxic effects of this drug at the transcriptomic, proteomic, and metabolomic levels. For this purpose, human hepatoma-derived HepaRG cells were exposed to a number of concentrations of bosentan during different periods of time. Bosentan was found to functionally and transcriptionally suppress the bile salt export pump as well as to alter bile acid levels. Pathway analysis of both transcriptomics and proteomics data identified cholestasis as a major toxicological event...
May 14, 2018: Archives of Toxicology
https://www.readbyqxmd.com/read/29761168/outcomes-of-surgical-management-of-familial-intrahepatic-cholestasis-1-and-bile-salt-export-protein-deficiencies
#7
Laura N Bull, Ludmila Pawlikowska, Sandra Strautnieks, Irena Jankowska, Piotr Czubkowski, Jennifer L Dodge, Karan Emerick, Catherine Wanty, Sami Wali, Samra Blanchard, Florence Lacaille, Jane A Byrne, Albertien M van Eerde, Kaija-Leena Kolho, Roderick Houwen, Steven Lobritto, Vera Hupertz, Patricia McClean, Giorgina Mieli-Vergani, Etienne Sokal, Philip Rosenthal, Peter F Whitington, Joanna Pawlowska, Richard J Thompson
Progressive familial intrahepatic cholestasis (PFIC) with normal circulating gamma-glutamyl transpeptidase levels can result from mutations in the ATP8B1 gene (encoding familial intrahepatic cholestasis 1 [FIC1] deficiency) or the ABCB11 gene (bile salt export protein [BSEP] deficiency). We investigated the outcomes of partial external biliary diversion, ileal exclusion, and liver transplantation in these two conditions. We conducted a retrospective multicenter study of 42 patients with FIC1 deficiency (FIC1 patients) and 60 patients with BSEP deficiency (BSEP patients) who had undergone one or more surgical procedures (57 diversions, 6 exclusions, and 57 transplants)...
May 2018: Hepatology Communications
https://www.readbyqxmd.com/read/29760211/updates-to-clostridium-difficile-spore-germination
#8
Travis J Kochan, Matthew H Foley, Michelle S Shoshiev, Madeline J Somers, Paul E Carlson, Philip C Hanna
Germination of Clostridium difficile spores is a crucial early requirement for colonization of the gastrointestinal tract. Likewise, C. difficile cannot cause disease pathologies unless their spores germinate into metabolically active, toxin-producing cells. Recent advances in our understanding of C. difficile spore germination mechanisms indicate that this process is both complex and unique. This review defines unique aspects of the germination pathways of C. difficile and compares them to two other well-studied organisms, Bacillus anthracis and Clostridium perfringens C...
May 14, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29757072/lactobacillus-plantarum-ys-3-prevents-activated-carbon-induced-constipation-in-mice
#9
Xin Zhao, Ruokun Yi, Yu Qian, Kun-Young Park
The aim of this study was to determine the effects of Lactobacillus plantarum YS-3 (LP-YS3) on activated carbon-induced constipation in Kunming mice. The results of the experiment show that the antigastric acid activity and bile salt tolerance of LP-YS3 were stronger than those of Lactobacillus bulgaricus (LB). LP-YS3 inhibited loss of body weight caused by constipation and further reductions in fecal weight, particle number, and water content in mice. Moreover, LP-YS3 elevated the gastrointestinal transit rate and reduced the time required for initial black stool defecation...
May 14, 2018: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29751880/update-on-ancillary-testing-in-the-evaluation-of-high-grade-liver-tumors
#10
REVIEW
Anne Koehne de Gonzalez, Stephen M Lagana
Tissue diagnosis is the gold standard for mass lesions of the liver, but needle core biopsies may sometimes prove challenging. Presented here is a review of a panel of immunohistochemical stains, including hepatocyte in paraffin 1, arginase-1, polyclonal carcinoembryonic antigen, CD10, bile salt export pump, glypican-3, as well as in situ hybridization for albumin RNA, to establish hepatocellular origin in cases in which hepatocellular carcinoma is suspected but the sample is limited or the morphology is challenging, as it may be with cases of scirrhous, fibrolamellar carcinoma, intrahepatic cholangiocarcinoma, and combined hepatocellular-cholangiocarcinoma...
June 2018: Surgical Pathology Clinics
https://www.readbyqxmd.com/read/29751655/a-comprehensive-genome-survey-provides-novel-insights-into-bile-salt-hydrolase-bsh-in-lactobacillaceae
#11
Lifeng Liang, Yunhai Yi, Yunyun Lv, Junwei Qian, Xuejing Lei, Gengyun Zhang
Bile salt hydrolase (BSH) is a well-known enzyme that has been commonly characterized in probiotic bacteria, as it has cholesterol-lowering effects. However, its molecular investigations are scarce. Here, we build a local database of BSH sequences from Lactobacillaceae (BSH⁻SDL), and phylogenetic analysis and homology searches were employed to elucidate their comparability and distinctiveness among species. Evolutionary study demonstrates that BSH sequences in BSH⁻SDL are divided into five groups, named BSH A, B, C, D and E here, which can be the genetic basis for BSH classification and nomenclature...
May 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29734330/sustained-activation-of-detoxification-pathways-promotes-liver-carcinogenesis-in-response-to-chronic-bile-acid-mediated-damage
#12
Agnese Collino, Alberto Termanini, Paola Nicoli, Giuseppe Diaferia, Sara Polletti, Camilla Recordati, Vittoria Castiglioni, Donatella Caruso, Nico Mitro, Gioacchino Natoli, Serena Ghisletti
Chronic inflammation promotes oncogenic transformation and tumor progression. Many inflammatory agents also generate a toxic microenvironment, implying that adaptive mechanisms must be deployed for cells to survive and undergo transformation in such unfavorable contexts. A paradigmatic case is represented by cancers occurring in pediatric patients with genetic defects of hepatocyte phosphatidylcholine transporters and in the corresponding mouse model (Mdr2-/- mice), in which impaired bile salt emulsification leads to chronic hepatocyte damage and inflammation, eventually resulting in oncogenic transformation...
May 7, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29733655/stepwise-aggregation-of-cholate-and-deoxycholate-dictates-the-formation-and-loss-of-surface-available-chirally-selective-binding-sites
#13
Adam R Meier, Jenna B Yehl, Kyle W Eckenroad, Gregory A Manley, Timothy G Strein, David Rovnyak
Bile salts are facially amphiphilic, naturally occurring chemicals that aggregate to perform numerous biochemical processes. Because of their unique intermolecular properties, bile salts have also been employed as functional materials in medicine and separation science (e.g. drug delivery, chiral solubilization, purification of single-walled carbon nanotubes). Bile micelle formation is structurally complex, and remains a topic of considerable study. Here, the exposed functionalities on the surface of cholate and deoxycholate micelles are shown to vary from one another and with micelle aggregation state...
May 7, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29731282/rethinking-reverse-cholesterol-transport-and-dysfunctional-high-density-lipoproteins
#14
REVIEW
Baiba K Gillard, Corina Rosales, Bingqing Xu, Antonio M Gotto, Henry J Pownall
Human plasma high-density lipoprotein cholesterol concentrations are a negative risk factor for atherosclerosis-linked cardiovascular disease. Pharmacological attempts to reduce atherosclerotic cardiovascular disease by increasing plasma high-density lipoprotein cholesterol have been disappointing so that recent research has shifted from HDL quantity to HDL quality, that is, functional vs dysfunctional HDL. HDL has varying degrees of dysfunction reflected in impaired reverse cholesterol transport (RCT). In the context of atheroprotection, RCT occurs by 2 mechanisms: one is the well-known trans-hepatic pathway comprising macrophage free cholesterol (FC) efflux, which produces early forms of FC-rich nascent HDL (nHDL)...
April 12, 2018: Journal of Clinical Lipidology
https://www.readbyqxmd.com/read/29719591/acidic-bile-salts-induces-mucosal-barrier-dysfunction-through-let-7a-reduction-during-gastric-carcinogenesis-after-helicobacter-pylori-eradication
#15
Yasushi Takahashi, Kaname Uno, Katsunori Iijima, Yasuhiko Abe, Tomoyuki Koike, Naoki Asano, Kiyotaka Asanuma, Tooru Shimosegawa
Gastric cancer (GC) after eradication for Helicobacter pylori ( H.pylori ) increases, but its carcinogenesis is not elucidated. It is mainly found in acid non-secretion areas (ANA), as mucosal regeneration in acid secretory areas (AA) after eradication changes the acidity and bile toxicity of gastric juice. We aimed to clarify the role of barrier dysfunction of ANA by the stimulation of pH3 bile acid cocktail (ABC) during carcinogenesis. We collected 18 patients after curative endoscopic resection for GC, identified later than 24 months after eradication, and took biopsies by Congo-red chromoendoscopy to distinguish AA and ANA (UMIN00018967)...
April 6, 2018: Oncotarget
https://www.readbyqxmd.com/read/29717168/allosteric-modulation-of-the-farnesoid-x-receptor-by-a-small-molecule
#16
Matthias Gabler, Jan Kramer, Jurema Schmidt, Julius Pollinger, Julia Weber, Astrid Kaiser, Frank Löhr, Ewgenij Proschak, Manfred Schubert-Zsilavecz, Daniel Merk
The bile acid activated transcription factor farnesoid X receptor (FXR) regulates numerous metabolic processes and is a rising target for the treatment of hepatic and metabolic disorders. FXR agonists have revealed efficacy in treating non-alcoholic steatohepatitis (NASH), diabetes and dyslipidemia. Here we characterize imatinib as first-in-class allosteric FXR modulator and report the development of an optimized descendant that markedly promotes agonist induced FXR activation in a reporter gene assay and FXR target gene expression in HepG2 cells...
May 1, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29704124/growth-and-cell-properties-of-modified-lactobacillus-plantarum-cicc21001-with-supplementing-c-18-ffas-to-growth-medium-in-vitro
#17
Qinke Shen, Yuxian Wang, Jian Shen, Ling Jiang, Ce Wei, Hongman Zhang
Fatty acids (FAs) are one of the important factors that can influence cell growth and membrane composition. The aim of this study was to investigate the influence of supplementing MLM+ growth medium with C18 free fatty acids (C18 -FFAs), including stearic (C18:0), oleic (C18:1), linoleic (C18:2), and linolenic (C18:3) acid, on the growth of Lactobacillus plantarum CICC21001 by forming ion pairs with lysine to increase the solubility of FAs in liquid medium. The utilization of C18 -FFAs was further confirmed by GC-FID...
April 27, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29678738/autochthonous-lactic-acid-bacteria-isolated-from-pig-faeces-in-thailand-show-probiotic-properties-and-antibacterial-activity-against-enteric-pathogenic-bacteria
#18
Wandee Sirichokchatchawan, Puwiya Pupa, Prasert Praechansri, Nutthee Am-In, Somboon Tanasupawat, Piengchan Sonthayanon, Nuvee Prapasarakul
Lactic acid bacteria (LAB) play an important role in pig health and performance that arises from their beneficial impacts on the balance of gastrointestinal microbes, ability to fight enteric pathogens, and capacity to support the immune system. The aim of this study was to evaluate the functional and safety aspects of five previously isolated autochthonous LAB strains, (Lactobacillus plantarum 22F, 25F and 31F, Pediococcus acidilactici 72N and Pediococcus pentosaceus 77F) from pig faeces as potential probiotics for a pig feed supplement...
April 17, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29676719/vibrio-gangliei-sp-nov-a-novel-member-of-vibrionaceae-isolated-from-sawdust-in-a-pigpen
#19
Yu-Chen Meng, Hong-Can Liu, Yu-Guang Zhou, Man Cai, Yingqian Kang
A Gram-stain-negative, rod-shaped, catalase- and oxidase-positive, facultatively anaerobic, and motile bacterium, designated strain SZDIS-1T , was isolated from pigpen sawdust bedding in Xiamen, Fujian Province, China. Cells grew at 10-50 °C, pH 6.0-9.0, up to 12 % (w/v) NaCl, resisted vibriostatic agent O/129 and were negative for gelatin and alginate hydrolysis. No growth on thiosulfate citrate bile salts sucrose agar medium. Based on 16S rRNA gene sequences and multilocus sequence analysis, this strain should be assigned to the genus Vibrio, with the closest relatives being Vibrio aphrogenes CA-1004T (97...
April 20, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29669842/bile-salt-dependent-lipase-promotes-intestinal-adaptation-in-rats-with-massive-small-bowel-resection
#20
Yi Yang, Tao Zheng, Jiefei Zhou, Huanlei Song, Wei Cai, Linxi Qian
Intestinal adaptation is important for the short bowel syndrome (SBS) patients. Growing evidence has suggested that bile salt dependent lipase (BSDL) not only has the lipolytic activity, but also the immune-modulating and pro-proliferative activities. The purpose of this study was to investigate the effects of BSDL on intestinal adaptive growth and gut barrier function in a rat model of SBS. Twenty-four male Sprague-Dawley rats were randomly divided into three experimental groups: sham group (rats underwent bowel transection and reanastomosis), SBS group (rats underwent 80 % bowel resection), SBS-BSDL group (SBS rats orally administered BSDL)...
April 18, 2018: Bioscience Reports
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