Read by QxMD icon Read

Gram's stain

Vinod Shanmughapriya, Stephen Richard, Tamilmaran Nagarajan, M Hussain Munavar
AIM: The ssrA mutants were found to be more sensitive to mitomycin C (MMC) and our aim was to study this phenomenon in detail. MATERIALS & METHODS: Strains were constructed by P1 transduction. pssrA(+) plasmid was constructed by PCR-based cloning and transformation was done by CaCl2 method. Relative viability analyses were done to assess the extent of viability of strains in relevant conditions. Gram staining was used for microscopic analysis. RESULTS: ssrA mutants become sensitive specifically to MMC, that too in a strain-specific manner...
October 13, 2017: Future Microbiology
Ajayi Adesola Adetutu, Bibi Oritsewehinmi, Oniha Margaret Ikhiwili, Atolagbe Oluwabunmi Moradeke, Anosike Selina Odochi, Onibokun Elizabeth Adeola
BACKGROUND AND OBJECTIVE: Pimples (acne) are small skin lesions or inflammations of the skin. The most common factor causing acne is the hormonal changes that occur during adolescent and teenage years. Antibiotics are becoming less effective in the treatment of pimples due to increasing concerns of antibiotic resistance. This study was therefore carried out to characterize the isolates from the pimples of Covenant University Students and to determine their antibiotics sensitivity pattern...
2017: Pakistan Journal of Biological Sciences: PJBS
Chengliang Cao, Bo Yuan, Sheng Qin, Jihong Jiang, Faxiang Tao, Bin Lian
A novel Gram-stain-positive, aerobic bacterium, designated strain DHS C021(T), was isolated from a limestone sample collected from the Puding Karst Ecosystem Research Station of Guizhou Province, southwest China. This strain developed branched vegetative mycelia, and its aerial mycelia fragmented into rod-shaped spores. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the whole-cell sugars comprised galactose, ribose and mannose. The respiratory quinone was identified as menaquinone MK-9(H4)...
October 12, 2017: International Journal of Systematic and Evolutionary Microbiology
Hu Liao, Yuqian Li, Mengjia Zhang, Xiaolan Lin, Qiliang Lai, Yun Tian
A Gram-stain-negative, facultative anaerobic, yellow-pigmented, rod-shaped, non-flagellum and non-motile bacterial strain, designed C9-11(T), was isolated from mangrove sediment in Zhangzhou, Fujian Province, China. Growth was observed at temperatures from 15 to 37 °C (optimum 30 °C), at salinities from 0 to 4 % (optimum 0.5-2 %) and at pH from 6 to 8 (optimum 7). Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain C9-11(T) was a member of the genus Altererythrobacter. Strain C9-11(T) was related most closely to Altererythrobacter oceanensis Y2(T) (96...
October 12, 2017: International Journal of Systematic and Evolutionary Microbiology
Jaewoo Yoon, Mina Yasumoto-Hirose, Hiroaki Kasai
A Gram-stain-negative, rod-shaped, non-motile, strictly aerobic, chemoheterotrophic, light-yellow-pigmented bacterium, designated strain 02OK1/10-76(T), was isolated from a mangrove estuary in Japan by use of an in situ cultivation technique. Preliminary analysis based on the 16S rRNA gene sequence revealed that the novel isolate was affiliated with the family Flavobacteriaceae of the phylum Bacteroidetes and that it showed highest sequence similarity (96.9 %) to Frondibacter aureus A5Q-67(T). The DNA G+C content of strain 02OK1/10-76(T) was 35 mol%; MK-6 was the only menaquinone; and iso-C15 : 0, iso-C17 : 0 3-OH and iso-C16 : 0 3-OH were the major (>10 %) cellular fatty acids...
October 12, 2017: International Journal of Systematic and Evolutionary Microbiology
Yaping Du, Xiupian Liu, Qiliang Lai, Weiwei Li, Fengqin Sun, Zongze Shao
A taxonomic study was carried out on strain L54-1-50(T), which was isolated from surface seawater of the South China Sea. Cells of strain L54-1-50(T) were Gram-stain-negative, rod-shaped, oxidase-positive and catalase-positive. Growth was observed at salinities from 0 to 9 % (optimum 2 %, w/v), at pH 6.0-10.0 (optimum 8.0-9.0) and at temperatures from 10 to 45 °C (optimum 25-37 °C), but not at 4 or 50 °C. The 16S rRNA gene sequence analysis indicated that strain L54-1-50(T) was a member of the genus Oceanibaculum, related to Oceanibaculum indicum P24(T) (98...
October 12, 2017: International Journal of Systematic and Evolutionary Microbiology
Yu Wang, Ding Xu, Anna Luo, Gejiao Wang, Shixue Zheng
A Gram-stain-positive, rod-shaped, non-spore-forming bacterium, designated as x-2(T), was isolated from lakeside soil of Sayram in the Xinjiang Uygur Autonomous Region, PR China. On the basis of 16S rRNA gene sequences, strain x-2(T) belongs to the genus Nocardioides in the family Nocardioidaceae, being most closely related to Nocardioides panacisoli Gsoil 346(T) (97.36 % 16S rRNA gene sequence similarity). Strain x-2(T) was characterized chemotaxonomically and found to have ll-diaminopimelic acid in the cell-wall peptidoglycan, phosphatidylglycerol, diphosphatidylglycerol, glycolipids and another three unknown phospholipids as the major polar lipids, MK-8(H4) as the predominant menaquinone and C18 : 1ω9c, iso-C16 : 0, C17 : 1ω8c and C16 : 0 as the major fatty acids...
October 2017: International Journal of Systematic and Evolutionary Microbiology
Ji Young Kang, Mi-Jung Kim, Jeesun Chun, Kyung Pyo Son, Kwang Yeop Jahng
A Gram-stain-negative, non-motile and facultative anaerobic bacterium, designated CAM-8(T), was isolated from an artificial fountain at Chonbuk National University, South Korea. The novel strain grew at 20-37 °C (optimum 25 °C), pH 5.5-7.0 (optimum 6.0) and with 0-2 % NaCl (optimum 0 %). Oxidase and catalase activities were positive. The cell morphology of strain CAM-8(T) was atypical rods 0.6-0.8 µm in width and 4.5-6.5 µm in length, with a peaked tip and sometimes a bulb shape. CAM-8(T) existed as single cells, and as pairs or chains of cells...
October 12, 2017: International Journal of Systematic and Evolutionary Microbiology
Patricia Lynn Shewmaker, Anne M Whitney, Christopher A Gulvik, Neil S Lipman
Three isolates of a previously reported novel catalase-negative, Gram-stain-positive, coccoid, alpha-haemolytic, Streptococcus species that were associated with meningoencephalitis in naïve weanling mice were further evaluated to confirm their taxonomic status and to determine additional phenotypic and molecular characteristics. Comparative 16S rRNA gene sequence analysis showed nearly identical intra-species sequence similarity (≥99.9 %), and revealed the closest phylogenetically related species, Streptococcus acidominimus and Streptococcus cuniculi, with 97...
October 12, 2017: International Journal of Systematic and Evolutionary Microbiology
Guishan Zhang, Mohamed Fauzi Haroon, Ruifu Zhang, Xiaoyan Dong, Dandan Wang, Yunpeng Liu, Weibing Xun, Xiuzhu Dong, Ulrich Stingl
Two moderately halophilic marine bacterial strains of the family Rhodobacteraceae, designated ZGT108(T) and ZGT118(T), were isolated from the brine-seawater interface at Erba Deep in the Red Sea (Saudi Arabia). Cells of both strains were aerobic, rod-shaped, non-motile, and Gram-stain-negative. The sequence similarity of the 16S rRNA genes of strains ZGT108(T) and ZGT118(T) was 94.9 %. The highest 16S rRNA gene sequence similarity of strain ZGT108(T) to its closest relative, Ruegeria conchae JCM 17315(T), was 98...
October 12, 2017: International Journal of Systematic and Evolutionary Microbiology
Leonid N Ten, Nabil Elderiny, Jae-Jin Lee, Seung-Yeol Lee, Sangkyu Park, Dae Sung Lee, Myung Kyum Kim, Hee-Young Jung
A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J8-9(T), was isolated from a sandy beach in Jeju Island, South Korea. The isolate was able to grow between 10 and 30 °C, pH 5-8, and in presence of 0-1% (w/v) NaCl. Based on 16S rRNA gene phylogenetic analysis, the novel strain was closely related to members of the genus Spirosoma (96.1-90.9% similarities) and showed highest sequence similarity to Spirosoma panaciterrae DSM 21099(T) (96.1%). The G + C content of the genomic DNA of strain 15J8-9(T) was 45...
October 11, 2017: Current Microbiology
Wei Fang, Jiuke Li, Hu-Shan Cui, Xiaohong Jin, Jing Zhai, Yuanmin Dai, Yumin Li
BACKGROUND: We present a case of post-traumatic endophthalmitis with relatively good prognosis caused by Gordonia sputi, which, to our knowledge is the first case in the literature. CASE PRESENTATION: A 24 year old man, who underwent an intraocular foreign body extraction half a month before presentation in the left eye, was referred to us complaining of blurred vision and slight pain for 5 days. His first presentation showed moderate intracameral and intravitreous purulent inflammation with a best corrected vision of counting fingers...
October 11, 2017: BMC Ophthalmology
Leonid N Ten, Jongguen Jeon, Su-Jin Park, Sangkyu Park, Seung-Yeol Lee, Myung Kyum Kim, Hee-Young Jung
A Gram-stain negative, non-motile, rod-shaped, aerobic bacterium, designated 15J8-8(T), was isolated from a soil sample collected on Jeju Island, South Korea, and characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 15J8-8(T) belongs to the family Cytophagaceae and is related to Larkinella bovis M2TB15(T) (95.0%), 'Larkinella harenae' 15J9-9 (94.5%), Larkinella arboricola Z0532(T) (93.2%), and Larkinella insperata LMG 22510(T) (93.0%). The DNA G+C content of strain 15J8-8(T) was 50...
October 10, 2017: Antonie Van Leeuwenhoek
Roshan Thapa, Narayan Dutt Pant, Uday Narayan Yadav, Eliza Thapa, Anjana Singh, Bharat Mani Pokhrel, Upendra Devkota
BACKGROUND: Patient's medical charts in hospitals are potentially contaminated by pathogenic bacteria and might act as vehicles for transmission of bacterial infections.This study was aimed to determine the rate of contamination of medical charts by multidrug resistant bacteria. METHODS: Sampling of total 250 patient's medical charts from different wards was done with the help of cotton swabs soaked in sterile normal saline. The swabs thus collected were cultured using standard microbiological procedures...
September 8, 2017: Journal of Nepal Health Research Council
Lijun Xi, Nenghu Qiao, Jingjing Zhang, Jing Li, Dejian Liu, Jing You, Jianguo Liu
A Gram-stain positive, non-motile, spherical, red-pigmented and facultatively anaerobic bacterium, designated strain 6.1(T), was isolated from a crude oil recovery water sample from the Huabei oil field in China. The novel strain exhibited tolerance of UV irradiation (> 1000 J m(-2)). Based on 16S rRNA gene sequence comparisons, strain 6.1(T) shows high similarity to Deinococcus citri DSM 24791(T) (98.1%) and Deinococcus gobiensis I-0(T) (97.8%), with less than 93.5% similarity to other closely related taxa...
October 9, 2017: Antonie Van Leeuwenhoek
M M Soltan Dallal, S Zamaniahari, A Davoodabadi, M Hosseini, Z Rajabi
Background: The pickle, a traditional fermented product, is popular among Iranians. Much research has been conducted worldwide on this food group. Due to a lack of related data in Iran, this study was conducted to isolate and identify dominant lactic acid bacteria (LAB) in pickles and salted pickles. Materials and methods: Seventy samples were collected from different regions of Iran. The isolated bacteria were identified as LAB by Gram staining and catalase by using MRS agar. Then, those strains were identified at the species level by physiological tests (e...
2017: GMS Hygiene and Infection Control
Lan Yang, Yun-Hao Wang, Hai-Zhen Zhu, Jiang-Baota Muhadesi, Bao-Jun Wang, Shuang-Jiang Liu, Cheng-Ying Jiang
Strain WY-1(T), a Gram-stain-negative, non-spore-forming, rod-shaped, non-motile bacterium, was isolated from the sewage treatment packing of a coking chemical plant. Strain WY-1(T) grew over a temperature range of 15-45 °C (optimum, 30-37 °C), a pH range of 5.5-11.0 (optimum, pH 6.5-7.0) and an NaCl concentration range of 0-3 % (w/v; optimum, 0 %). 16S rRNA gene sequence analysis showed that strain WY-1(T)was closely related to Parapedobacter indicus RK1(T) with the highest sequence similarity of 96...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Bao-Zhu Fang, Zheng-Shuang Hua, Ming-Xian Han, Zi-Tong Zhang, Yi-Huan Wang, Zi-Wen Yang, Wan-Qin Zhang, Min Xiao, Wen-Jun Li
A novel actinobacterial strain, designated SYSU K10005(T), was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic and Gram-stain-positive. On the basis of 16S rRNA gene sequence analysis, strain SYSU K10005(T) was most closely related to the type strains of the genus Nonomuraea, and shared highest sequence similarity of 98.4 % with Nonomuraea candida HMC10(T)...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Yang Liu, Nannan Li, Mi Kyung Eom, Peter Schumann, Xin Zhang, Yanhua Cao, Yuanyuan Ge, Ming Xiao, Jiuran Zhao, Chi Cheng, Song-Gun Kim
Two Gram-stain-positive bacterial strains, designated as 5L6(T) and 6L6, isolated from seeds of hybrid maize (Zea mays L., Jingke 968) were investigated using a polyphasic taxonomic approach. The cells were aerobic, motile, endospore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates were recognized as a species of the genus Bacillus, to which the five closest neighbours are Bacillus solani FJAT-18043(T) (99.8 % similarity), Bacillus horneckiae DSM 23495(T) (97...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Ji-Ru Han, Kan Wang, Jing Zhang, Guan-Jun Chen, Zong-Jun Du
A Gram-stain-negative, aerobic, non-motile, coccoid, ovoid or rod-shaped, and orange-pigmented bacterial strain, designated WS2-14(T), was isolated from sediment collected from a sea cucumber culture pond located in Rongcheng, Shandong province, PR China (122° 14' E, 36° 54' N). Strain WS2-14(T) grew optimally at 28 °C and pH 7.0-7.5, and was able to tolerate salt concentrations of 0.5-6.0 % (w/v) NaCl. Strain WS2-14(T) was characterized chemotaxonomically as possessing menaquinone-6 (MK-6) as the major respiratory quinone, as well as iso-C13 : 0, iso-C15 : 0 3-OH and iso-C15 : 0 as the predominant fatty acids...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"