Read by QxMD icon Read

Gram's stain

Chien-Hsun Huang, Jong-Shian Liou, Ai-Yun Lee, Min Tseng, Mika Miyashita, Lina Huang, Koichi Watanabe
Two Gram-stain-positive, rod-shaped, non-motile, catalase-negative and facultative anaerobic strains, NCYUAST and BCRC 18859 (=NRIC 1947), were isolated from cow manure of Taiwan and coconut juice of Philippines, respectively. Comparative sequence analysis of 16S rRNA gene revealed that the novel strains were members of the genus Lactobacillus. These two strains had 100% of 16S rRNA gene sequence similarity and 98.6% of average nucleotide identity (ANI) value based on whole genome sequences. On the basis of 16S rRNA gene sequence similarity, the type strains of Lactobacillus casei (99...
February 7, 2018: Systematic and Applied Microbiology
Chao Jia, Hong-Chang Cui, Yan-Qiong Han, Tian-Yu Fu, Rui Du, Xiao-Lei Wang, Xiao-Chong Shi, Xiao-Hua Zhang
A Gram-stain negative, obligately anaerobic, non-motile, asporogenous long rod-shaped and non-flagellated bacterial strain, designated 4SWWS2-6 T , was isolated from sediment in the intertidal zone of Fildes Peninsula, Antarctica. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 4SWWS2-6 T belongs to the genus Ancylomarina and showed high sequence similarity with Ancylomarina subtilis FA102 T (96.5%). Optimal growth occurred at pH 6.5, 16 °C and in the presence of 3% (w/v) NaCl...
February 17, 2018: Antonie Van Leeuwenhoek
Khoudia Diop, Awa Diop, Anthony Levasseur, Oleg Mediannikov, Catherine Robert, Nicholas Armstrong, Carine Couderc, Florence Bretelle, Didier Raoult, Pierre-Edouard Fournier, Florence Fenollar
Microbial culturomics is a new subfield of postgenomic medicine and omics biotechnology application that has broadened our awareness on bacterial diversity of the human microbiome, including the human vaginal flora bacterial diversity. Using culturomics, a new obligate anaerobic Gram-stain-negative rod-shaped bacterium designated strain khD1 T was isolated in the vagina of a patient with bacterial vaginosis and characterized using taxonogenomics. The most abundant cellular fatty acids were C 15:0 anteiso (36%), C 16:0 (19%), and C 15:0 iso (10%)...
February 15, 2018: Omics: a Journal of Integrative Biology
Etheresia Pretorius, Martin J Page, Lisa Hendricks, Nondumiso B Nkosi, Sven R Benson, Douglas B Kell
In recent work, we discovered that the presence of highly substoichiometric amounts (10 -8 molar ratio) of lipopolysaccharide (LPS) from Gram-negative bacteria caused fibrinogen clotting to lead to the formation of an amyloid form of fibrin. We here show that the broadly equivalent lipoteichoic acids (LTAs) from two species of Gram-positive bacteria have similarly (if not more) potent effects. Using thioflavin T fluorescence to detect amyloid as before, the addition of low concentrations of free ferric ion is found to have similar effects...
February 2018: Journal of the Royal Society, Interface
Rattan S Rashpa, Vikram K Mahajan, Pankaj Kumar, Karaninder S Mehta, Pushpinder S Chauhan, Ritu Rawat, Vikas Sharma
Background: Chronic kidney disease (CKD)-associated mucocutaneous manifestations significantly impair the quality of life but often remain understudied. They may also vary across regions, socioeconomic and nutritional status, and racial differences. Objectives: To study the patterns of mucocutaneous disorders and their prevalence in CKD patients irrespective of clinical stage or dialysis status. Materials and Methods: 122 (M:F = 77:45) patients aged 21‒85 (Mean ± SD = 57...
January 2018: Indian Dermatology Online Journal
Satish Kumar Rajasekharan, Jin-Hyung Lee, Yueju Zhao, Jintae Lee
Yeast-mold mycobiota inhabit several natural ecosystems, in which symbiotic relationships drive strategic pathoadaptation. Mycotoxins are metabolites produced by diverse mycotoxigenic fungi as a defense against yeasts, though at times yeasts secrete enzymes that degrade, detoxify, or bio-transform mycotoxins. The present study is focused on the in vitro inhibitory effects of zearalenone (ZEN), a F2 mycotoxin produced by several Fusarium and Gibberella species, on different microbial strains. ZEN exhibited no effect on the planktonic growth or biofilms of several Gram positive and negative bacteria at the tested concentrations...
March 2018: Indian Journal of Microbiology
Thirupathaiah Yeruva, Hemalatha Rajkumar, Vasundhara Donugama
Background & objectives: Lactobacilli species that are better adapted to vaginal environment of women may colonize better and offer protection against vaginal pathogenic bacteria. In this study, the distribution of common Lactobacillus species was investigated in pregnant women. Methods: Sixty seven pregnant women were included in the study and vaginal samples were collected for Gram staining. Women were classified as normal vaginal flora, intermediate flora and bacterial vaginosis (BV) based on Nugent's score...
October 2017: Indian Journal of Medical Research
M Linares Rufo, L Santos Larrégola, T Vela González, R Muñoz Bieber, E Barahora Ruíz
No abstract text is available yet for this article.
February 7, 2018: Semergen
Naiya B Patel, Jorge A Hinojosa, Meifang Zhu, Danielle M Robertson
Purpose: We have previously shown that invasive strains of Pseudomonas aeruginosa exploit the robust neutrophil response to form biofilms on contact lens surfaces and invade the corneal epithelium. The present study investigated the ability of multiple bacterial genera, all commonly recovered during contact lens-related infectious events, to adhere to and form biofilms on contact lens surfaces in the presence of neutrophils. Methods: Five reference strains from the American Type Culture Collection were used: P...
2018: Molecular Vision
Wouter S P Jong, Maaike Schillemans, Corinne M Ten Hagen-Jongman, Joen Luirink, Peter van Ulsen
Monomeric autotransporters have been extensively used for export of recombinant proteins to the cell surface of Gram-negative bacteria. A bottleneck in the biosynthesis of such constructs is the passage of the outer membrane, which is facilitated by the β-domain at the C terminus of an autotransporter in conjunction with the Bam complex in the outer membrane. We have evaluated eight β-domain constructs for their capacity to secrete fused proteins to the cell surface. These constructs derive from the monomeric autotransporters Hbp, IgA protease, Ag43 and EstA and the trimeric autotransporter Hia, which all were selected because they have been previously used for secretion of recombinant proteins...
2018: PloS One
Huifang Lim, Terence Ho, Melanie Kjarsgaard, Ann Efthimiadis, Deborah Yamamura, Parameswaran Nair
BACKGROUND: Salivary contamination decreases the yield of identifying potential airway bacterial and fungal pathogens in routine cultures of spontaneous or induced sputum during exacerbations of airway diseases. We investigated the utility of selecting out the squamous cells from sputum using inverted microscopy. METHODS: Sputum was obtained from fifty subjects, then divided to facilitate parallel processing of paired samples for gram-stain and semi-quantitative cultures, and quantitative cytometry...
January 2018: Respiratory Medicine
Sumreen Hayat, Saima Muzammil, Muhammad Hidayat Rasool, Zonaira Nisar, Syed Zajif Hussain, Anjum Nasim Sabri, Saba Jamil
The aim of the current investigation was to determine antibacterial and antibiofilm potential of magnesium oxide (MgO) nanoparticles against antibiotic resistant clinical strains of bacteria. MgO nanoparticles were synthesized by wet chemical method, and were further characterized by scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). Antibacterial activity was determined by broth microdilution and agar diffusion method. Bradford method was used to assess cellular protein leakage as a result of loss of membrane integrity...
February 6, 2018: Microbiology and Immunology
Bo Wu, Su-Hong Fu, Huan Tang, Kai Chen, Qiang Zhang, Ai-Hua Peng, Hao-Yu Ye, Xing-Jun Cheng, Mao Lian, Zhen-Ling Wang, Li-Juan Chen
Staphylococcus aureus is a major and dangerous human pathogen that causes a range of clinical manifestations of varying severity, and is the most commonly isolated pathogen in the setting of skin and soft tissue infections, pneumonia, suppurative arthritis, endovascular infections, foreign-body associated infections, septicemia, osteomyelitis, and toxic shocksyndrome. Honokiol, a pharmacologically active natural compound derived from the bark of Magnolia officinalis, has antibacterial activity against Staphylococcus aureus which provides a great inspiration for the discovery of potential antibacterial agents...
June 8, 2017: Bioorganic & Medicinal Chemistry Letters
Jens-Ole Bartz, Jochen Blom, Hans-Jürgen Busse, Jacques B Mvie, Martin Hardt, Patrick Schubert, Thomas Wilke, Alexander Goessmann, Gottfried Wilharm, Jennifer Bender, Peter Kämpfer, Stefanie P Glaeser
Two Gram-stain-negative, facultative anaerobic, motile, rod-shaped strains, S-B4-1UT and JOB-63a, forming small whitish transparent colonies on marine agar, were isolated from a sponge of the genus Haliclona. The strains shared 99.7% 16S rRNA gene sequence identity and a DNA-DNA hybridization value of 100%, but were differentiated by genomic fingerprinting using rep-PCRs. 16S rRNA gene sequence phylogeny placed the strains as a sister branch to the monophyletic genus Endozoicomonas (Oceanospirillales; Gammaproteobacteria) with 92...
December 22, 2017: Systematic and Applied Microbiology
Ya-Wen Sun, Jie Liu, De-Chao Zhang
A Gram-stain negative, rod-shaped, strictly aerobic, motile bacterial strain, designated YC239T, was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain YC239T is related to the genus Ponticaulis and has high 16S rRNA gene sequence similarity with the type strain of Ponticaulis koreensis GSW-23T (97.9%). The predominant cellular fatty acids were C18:1ω7c, C16:0, C17:0 and C17:1ω6c. Strain YC239T had Q-10 as the predominant ubiquinone...
February 1, 2018: Antonie Van Leeuwenhoek
N Hema, N Sunil Raj, E D Chaithanya, Rashmi Chincholi, M Iswariya, K N Hema
Aim: The aim of this study is to evaluate the prevalence of nasal carriage of coagulase positive methicillin-resistant Staphylococcus aureus (MRSA) among dental students. Materials and Methods: Four hundred dental students, divided into two groups - undergraduates (200) and postgraduates (200), were screened using sterile cotton swabs for carriage of MRSA in anterior nares. The samples were inoculated on HiCrome MeReSa Agar Base-with cefoxitin supplement. Further confirmatory tests were done by Gram-staining, Tube coagulase and Cefoxitin disc diffusion test on Mueller-Hinton agar...
September 2017: Journal of Oral and Maxillofacial Pathology: JOMFP
Benjamin D Welling, Lee S Haruno, Scott B Rosenfeld
BACKGROUND: Septic arthritis is frequently associated with adjacent infections including osteomyelitis and subperiosteal and intramuscular abscesses. While often clinically indiscernible from isolated septic arthritis, the diagnosis of adjacent infections is important in determining the need for additional surgical intervention. MRI has been used as the diagnostic gold standard for assessing adjacent infection. Routine MRI, however, can be resource-intensive and delay surgical treatment...
January 2018: Clinical Orthopaedics and related Research
Zheng-Fei Yan, Pei Lin, Kyung-Hwa Won, Chang-Tian Li, GyungSoo Park, ByungSun Chin, MooChang Kook, Qi-Jun Wang, Tae-Hoo Yi
A Gram-stain-negative, aerobic, non-motile, rod-shaped, catalase-positive and oxidase-positive bacteria (THG-T61T), was isolated from rhizosphere of Hibiscus syriacus. Growth occurred at 10-37 °C (optimum 25-30 °C), at pH 5.0-9.0 (optimum 7.0) and in the presence of 0-2.0 % NaCl (optimum without NaCl supplement). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T61T were identified as Sphingomonas ginsengisoli KCTC 12630T (97.9 %), Sphingomonas jaspsi DSM 18422T (97...
February 2018: International Journal of Systematic and Evolutionary Microbiology
Y Subhash, Sang-Seob Lee
Two dark pink pigmented bacterial strains (M3T and M11) were isolated from crude oil contaminated desert sand from Kuwait. Both strains were Gram-stain-negative and small-rod to oval-shaped bacteria. Strains M3T and M11 grew at 13-42 °C (optimum, 30-35 °C) and pH 6.5-9.0 (optimum, 7.0-7.5). No additional NaCl was required for the growth of both strains. The genomic DNA G+C content of strains M3T and M11 were 69.5 and 69.0 mol%, respectively. Both strains were closely related and the mean DNA-DNA hybridization value was 92±1 %...
February 2018: International Journal of Systematic and Evolutionary Microbiology
Sooyeon Park, Su Jung Choi, Sung-Min Won, Jung-Hoon Yoon
A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated JSSK-16T, was isolated from the place where the ocean and a freshwater spring meet at Jeju Island, South Korea. Strain JSSK-16T grew optimally at 30 °C, at pH 6.5-8.0 and in the presence of 2.0-4.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain JSSK-16T joined the clade comprising the type strains of Jannaschia species. Strain JSSK-16T exhibited 16S rRNA gene sequence similarity values of 97...
February 2018: International Journal of Systematic and Evolutionary Microbiology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"