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Gram's stain

P K Holyoake, A Thompson
CASE REPORT: This report describes an investigation into the cause of abortions on a commercial pig farm in Victoria in October 2015 in which six sows aborted over a 2-month period. Four of the abortions occurred in the 3 weeks prior to the sows' anticipated farrowing dates and the other two occurred in the second trimester of pregnancy. An analysis of farm data showed that the abortion rate in the previous 12 months (2014-15) was more than twice that of the previous 2 years (1...
December 2017: Australian Veterinary Journal
Haiyuan Cai, Henglin Cui, Yonghui Zeng, Yanan Wang, Helong Jiang
A bacterial strain, 1-14T, was isolated from cyanobacterial aggregates in a eutrophic lake, Taihu Lake, China. Cells were observed to be slightly curved, rod-shaped, aerobic and Gram-stain-negative. Optimal growth occurred at pH 7.0 (range: 5.0-9.0), 28 °C (range: 20-32 °C) and 0 % (w/v) NaCl (range: 0-1.0 %) in R2A broth. No growth is observed at 37 °C. The cells were found to be positive for oxidase and catalase activities. The major respiratory quinone was ubiquinone Q-10. The major fatty acids (>10 %) were identified as C18 : 1ω6c/C18 : 1ω7c, C16 : 0 3-OH and C18 : 1 2-OH...
December 14, 2017: International Journal of Systematic and Evolutionary Microbiology
Sarabjeet Kour Sudan, Deepika Pal, Bhawana Bisht, Narender Kumar, Vasvi Chaudhry, Prabhu Patil, Girish Sahni, Shanmugam Mayilraj, Srinivasan Krishnamurthi
A bacterial strain, designated ASS-1T, was isolated and identified from a sediment sample of the river Ganges, Allahabad, India. The strain was Gram-stain-negative, formed straw-yellow pigmented colonies, was strictly aerobic, motile with a single polar flagellum, and positive for oxidase and catalase. The major fatty acids were C16 : 1ω7c/ 16 : 1 C16 : 1ω6c, C18 : 1ω7c and C16 : 0. Sequence analysis based on the 16S rRNA gene revealed that strain ASS-1T showed high similarity to Pseudomonas guguanensis CC-G9AT (98...
December 14, 2017: International Journal of Systematic and Evolutionary Microbiology
Babak Haghshenas, Yousef Nami, Ali Almasi, Norhafizah Abdullah, Dayang Radiah, Rozita Rosli, Abolfazl Barzegari, Ahmad Yari Khosroushahi
Background and Objectives: Probiotics are live microorganisms, which show beneficial health effects on hosts once consumed in sufficient amounts. LAB group can be isolated and characterized from traditional dairy sources. This study aimed at isolating, identifying, and in vitro characterizing (low pH/high bile salt tolerance, antibacterial activity, and antibiotic susceptibility) LAB strains from traditional Iranian dairy products. Materials and Methods: Isolated strains were identified by Gram staining, catalase assay, and 3 molecular identification methods; namely, (GTG) 5-PCR fingerprinting, ARDRA, and 16S rDNA gene sequencing...
August 2017: Iranian Journal of Microbiology
Stephen M Brecher
Our mostly manual, agar based clinical microbiology laboratory is slowly but steadily being re-defined by automation and innovation. Ironically, the oldest test, the Gram stain, is still manually read and interpreted by trained personnel. In this proof of concept study Smith et al. ( have used computer imaging with a deep convolutional neural network to examine and interpret Gram-stained slides from positive blood culture bottles. In light of the shortage of medical technologists/microbiologists and the need for results of positive blood culture bottles 24/7, this paper paves the way for the next innovations for the clinical microbiology laboratory of the future...
December 13, 2017: Journal of Clinical Microbiology
Erik Munson
"The previous era was characterized by house staff laboratories in every ward, and house staff and attending physicians often spent long periods performing a diagnostic examination of the expectorated sputum specimen, which had been obtained with great care and plated for prompt incubation in incubators on the ward. Therapeutic decisions were generally based on these results. During the past 30 years, there has been a notable decline in the quality of this exercise. Some of this decline can be ascribed to the Clinical Laboratory Improvement Amendments of 1988, which required that staff have credentials to interpret Gram stains of any specimens, thus essentially eliminating the house staff laboratory...
December 13, 2017: Journal of Clinical Microbiology
Richard H Asmah, Harriet N A Blankson, Kekeli A Seanefu, Noah Obeng-Nkrumah, Georgina Awuah-Mensah, Momodou Cham, Patrick F Ayeh-Kumi
BACKGROUND: Trichomonas vaginalis (TV) infection is the most prevalent non-viral sexually transmitted pathogen worldwide. Among pregnant women, the infection may cause adverse birth outcomes such as premature rupture of membranes and premature labour. In view of the paucity of information relating to TV among Ghanaian pregnant women, this study investigated its prevalence and associated co-infections among pregnant women. METHODS: High vaginal swabs were obtained from 99 pregnant women using sterile cotton swab sticks...
December 13, 2017: BMC Women's Health
Nenghu Qiao, Lijun Xi, Jingjing Zhang, Dejian Liu, Baosheng Ge, Jianguo Liu
A bacterial strain, K11T, capable of degrading phenol derivatives was isolated from activated sludge of a sewage treatment plant in China. This strain, which can degrade more than ten phenol derivatives, was identified as a Gram-stain negative, rod-shaped, asporogenous, facultative anaerobic bacterium with a polar flagellum. The strain was found to grow in tryptic soy broth in the presence of 0-2.5% (w/v) NaCl (optimum 0-1%), at 4-43 °C (optimum 30-35 °C) and pH 4.5-10.5 (optimum 7.5-8). Comparative analysis of nearly full-length 16S rRNA gene sequences showed that this strain belongs to the genus Thauera...
December 13, 2017: Antonie Van Leeuwenhoek
Shamsun Nahar, Chang-Jun Cha
An aerobic, Gram-staining-variable, rod-shaped, endospore-forming and motile bacterial strain, designated CJ6T, was isolated from a tidal flat on Ganghwa Island, South Korea. The isolate was characterized based on a polyphasic taxonomy approach. Strain CJ6T grew optimally on R2A agar media at 30 °C and pH 7. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CJ6T belonged to the genus Paenibacillus, displaying the highest sequence similarity to Paenibacillus vulneris CCUG 53270T (97...
December 13, 2017: International Journal of Systematic and Evolutionary Microbiology
Atsuko Ueki, Kazushi Goto, Nobuo Kaku, Katsuji Ueki
A strictly anaerobic bacterial strain (FH042T) was isolated from a methanogenic reactor treating waste from cattle farms. Cells were stained Gram-positive, straight to gently curved rods with polar flagella. The strain was asaccharolytic. The strain fermented amino acids (l-arginine, l-lysine and l-serine) as growth substrates and produced acetate and butyrate. The optimum temperature for growth was 30 °C and the optimum pH was 6.1-6.8. Oxidase, catalase and nitrate-reducing activities were negative. Hydrogen sulfide was produced...
December 13, 2017: International Journal of Systematic and Evolutionary Microbiology
Minglu Shao, Yang Li, Huafeng Cui, Man Jiang, Qiwen Tan
OBJECTIVE: To observe the protective effect of acupuncture preconditioning at "Jiaji" (EX-B 2) on acute myocardial ischemia-reperfusion injury (MIRI) in the rats and explore the mechanism. METHODS: Fifty Wistar rats were randomly divided into a control group, a model group, a Jiaji group, a Neiguan group and a Quchi group, 10 rats in each one. In the Jiaji group, the Neiguan group and the Quchi group, electroacupuncture was given for preconditioning at "Jiaji" T4~T5 (EX-B 2), "Neiguan" (PC 6) and "Quchi" (LI 11) for 7 days before modeling...
March 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
Folarin A Oguntoyinbo, Margo Cnockaert, Gyu-Sung Cho, Jan Kabisch, Horst Neve, Wilhelm Bockelmann, Mareike Wenning, Charles M A P Franz, Peter Vandamme
A Gram-stain-negative, rod-shaped Proteobacteria isolate, MBT G8648T, was obtained from an acid curd cheese called Quargel. The isolate was moderately salt tolerant and motile, with numerous peritrichous flagella. The 16S rRNA gene sequence analysis indicated that the strain belongs to the genus Halomonas, with 98.42 % 16S rRNA gene sequence similarity with Halomonas titanicae BH1T as nearest related neighbour. Further comparative sequence analysis of secA and gyrB genes, as well as physiological and biochemical tests, revealed that this bacterium formed a taxon well-separated from its nearest neighbours and other established Halomonas species...
December 12, 2017: International Journal of Systematic and Evolutionary Microbiology
Bo Zhang, Zhi-Qiang Liu, Yu-Guo Zheng
A Gram-stain-negative, aerobic, rod-shaped, bacterium, C62-2T, was isolated from activated sludge in Fujian Province, China. Phylogenetic analysis of the 16S rRNA gene sequences showed that it was closely related to Pedobacter duraquae WB 2.1-25T (97.92 %), Pedobacter bambusae THG-G118T (97.40 %), Pedobacter cryoconitis A37T (97.37 %) and Pedobacter caeni LMG 22862T (97.3 %). Cells grew aerobically at 20-37 °C (optimum, 30 °C), pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-3.0 % (w/v) NaCl...
December 12, 2017: International Journal of Systematic and Evolutionary Microbiology
Robert D Kirkton, Heather L Prichard, Maribel Santiago-Maysonet, Laura E Niklason, Jeffrey H Lawson, Shannon L M Dahl
BACKGROUND: Synthetic expanded polytetrafluorethylene (ePTFE) grafts are routinely used for vascular repair and reconstruction but prone to sustained bacterial infections. Investigational bioengineered human acellular vessels (HAVs) have shown clinical success and may confer lower susceptibility to infection. Here we directly compared the susceptibility of ePTFE grafts and HAV to bacterial contamination in a preclinical model of infection. MATERIALS AND METHODS: Sections (1 cm2) of ePTFE (n = 42) or HAV (n = 42) were inserted within bilateral subcutaneous pockets on the dorsum of rats and inoculated with Staphylococcus aureus (107 CFU/0...
January 2018: Journal of Surgical Research
Ram Hari Dahal, Jaisoo Kim
Two isolates of bacterial strains A15T and A17 were isolated from stream bank soil in Kyonggi University. Cells were aerobic, Gram-stain-negative, oxidase- and catalase-positive, motile, non-spore-forming, rod-shaped, opaque, and cream coloured. Both strains hydrolysed CM-cellulose. Strains were able to grow at 20-42 °C, pH 5.5-10.0 and at 1.5 % NaCl concentration (w/v). Indole test was positive. Analyses of phylogenetic trees based on its 16S rRNA gene sequences indicated that strain A15T formed a lineage within the family Rhodospirillaceae of the phylum Proteobacteria which was distinct from Ferrovibrio denitrificans S3T (98...
December 8, 2017: International Journal of Systematic and Evolutionary Microbiology
Wen-Ming Chen, Tsai-Ying Hsieh, Shih-Yi Sheu
A pink-coloured bacterial strain, TAPP7T, was isolated from a freshwater creek in Taiwan. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TAPP7T belonged to the genus Mucilaginibacter and showed highest similarity with Mucilaginibacter ginsengisoli B4Y-8T (97.6 %) and Mucilaginibacter carri PR0008KT (96.9 %). Cells of strain TAPP7T were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate-accumulating and short-rod-shaped. Growth occurred at 10-30 °C (optimum, 15-20 °C), at pH 4-8 (optimum, pH 6) and with 0-1 % NaCl (optimum, 0...
December 8, 2017: International Journal of Systematic and Evolutionary Microbiology
Meng-Meng Huang, Li-Li Guo, Yue-Hong Wu, Qi-Liang Lai, Zong-Ze Shao, Chun-Sheng Wang, Min Wu, Xue-Wei Xu
A Gram-stain-negative, rod-shaped bacterium, designated 157T, was isolated from seawater collected from the Philippine Sea. Cells of strain 157T grew in medium containing 0.5-10.0 % NaCl (w/v, optimum 3 %), at pH 6.0-8.5 (optimum 7.0) and at 15-40 °C (optimum 30 °C). Tweens 20, 40 and 80 as well as urea were hydrolysed. The 16S rRNA gene sequence of strain 157T had a high sequence similarity with respect to Pseudooceanicola marinus AZO-CT (97.2 %), and exhibited less than 97.0 % sequence similarity to other type strains of the species with validly published names...
December 8, 2017: International Journal of Systematic and Evolutionary Microbiology
Young Joon Oh, Ja-Young Jang, Seul Ki Lim, Min-Sung Kwon, Jieun Lee, NamHee Kim, Mi-Young Shin, Hyo Kyeong Park, Myung-Ji Seo, Hak-Jong Choi
A Gram-stain-positive, halophilic, rod-shaped, non-motile, spore forming bacterium, strain NKC1-2T, was isolated from kimchi, a Korean fermented food. Comparative analysis based on 16S rRNA gene sequence demonstrated that the isolated strain was a species of the genus Virgibacillus. Strain NKC1-2T exhibited high level of 16S rRNA gene sequence similarity with the type strains of Virgibacillus xinjiangensis SL6-1T (96.9%), V. sediminis YIM kkny3T (96.8%), and V. salarius SA-Vb1T (96.7%). The isolate grew at pH 6...
December 2017: Journal of Microbiology / the Microbiological Society of Korea
Joseph Okiria, Leonid N Ten, Su-Jin Park, Seung-Yeol Lee, Dong Hoon Lee, In-Kyu Kang, Dae Sung Lee, Hee-Young Jung
A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J9-8T, was isolated from soil on Jeju Island, Republic of Korea. The isolate was able to grow between 10 and 30°C, pH 6.5-8.5, and in presence of 0-1% (w/v) NaCl. The results of comparative 16S rRNA gene sequence analysis indicated that strain 15J9-8T represented a member of the family Cytophagaceae, phylum Bacteroidetes, and was most closely related to Spirosoma aerophilum 5516J-17T (96.1% similarity), Spirosoma pulveris JSH5-14T (95...
December 2017: Journal of Microbiology / the Microbiological Society of Korea
Lucas Senhorinho Esteves, Águida Cristina Gomes Henriques, Carolina Ávila Varginha de Moraes E Silva, Maria Cristina Teixeira Cangussu, Eduardo Antônio Gonçalves Ramos, Carlos Estrela, Jean Nunes Dos Santos
Periapical actinomycosis caused by a gram-positive anaerobic pathogen characterizes a typical extra-radicular infection. This study determined the frequency and correlated the content of bacteria colonies with the of periapical actinomycosis size. The study comprised a total of 218 periapical lesions (PL) (cysts, granulomas or abscess). The specimens embedded in paraffin were sliced into 4-µm sections and stained with hematoxylin-eosin, Gram, Periodic Acid-Schiff (PAS) and Grocott's stain. The presence of bacterial colonies composed of filamentous structures labeled with the histochemical stains were described as Actinomyces, and for each case, the bacterial colonies were counted and measured...
November 2017: Brazilian Dental Journal
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