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Gram's stain

Yos Adi Prakoso, Kurniasih
The aim of this study is to explore the effect of topical application of Aloe vera on skin wound healing. Thirty-six male Sprague-Dawley rats weighing 150-200 grams were divided into four groups. All groups were anesthetized, shaved, and exposed to round full-thickness punch biopsy on the back: group I (control); group II (treated with 1% Aloe vera cream); group III (treated with 2% Aloe vera cream); and group IV (treated with madecassol®). The treatments were given once a day. Macroscopic and microscopic examination were observed at 5, 10, and 15 days after skin biopsy...
2018: Journal of Tropical Medicine
David J Castro, Isabel Cerezo, Inmaculada Sampedro, Fernando Martínez-Checa
Strain D15T was isolated from a soil sample taken from Rambla Salada (Murcia), south-eastern Spain, by using the dilution-to-extinction method. The strain, a Gram-stain-negative aerobic bacteria, is non-motile, ovoid- or rod-shaped, catalase- and oxidase-positive, and grows at NaCl concentrations within the range 0.5-10 % (w/v) [optimum 3 % (w/v)], at 5-30 °C (optimum 28 °C) and at pH 6-9 (optimum pH 7.0). The 16S rRNA gene sequence indicates that it belongs to the genus Roseovarius in the class Alphaproteobacteria...
April 17, 2018: International Journal of Systematic and Evolutionary Microbiology
Sumi Akter, Mann Kyoon Shin
A Gram-stain-negative, oxidase-negative and catalase-negative, motile, rod-shaped bacterial strain, designated PCS8T , hosted by the ciliate Paramecium caudatum was investigated by using a polyphasic approach. Strain PCS8T was observed to be able to grow at 12-44 °C (optimum, 36-37 °C), at pH 6.0-10.0 (optimum, 7.0) and in the presence of 0-3 % NaCl (optimum, 1-2 %). It could hydrolyse starch and aesculin and produce acid from d-sorbitol, myo-inositol, glycerol and l-rhamnose. The sequence similarity of the new isolate was 96...
April 17, 2018: International Journal of Systematic and Evolutionary Microbiology
Feng-Qing Wang, Li-Hua Ren, Rong-Jie Zou, Yu-Zeng Sun, Xiao-Jing Liu, Fang Jiang, Li-Juan Liu
A yellow-pigmented bacterial strain (JR1T ) isolated from a sediment sample was subjected to a taxonomic study, based on phenotypic, genetic and physiological characterization. Here, we describe the cultivation and characteristics of strain JR1T , a novel member of the genus Carboxylicivirga in the family Marinilabiliaceae. Cells of strain JR1T were rod-shaped, Gram-stain-negative, non-motile and facultatively anaerobic. The temperature range for growth was 15-42 °C (optimum, 33 °C) and the pH range for growth was pH 6...
April 16, 2018: International Journal of Systematic and Evolutionary Microbiology
Abeer Mostafa Abdalhamed, Gamil Sayed Gamil Zeedan, Hala Abdoula Ahmed Abou Zeina
Aim: The present work aims to isolate and identify bacteria that cause mastitis in small ruminants and evaluates the antibacterial activity of some antibiotics, honey, essential oils, and plant extracts. Materials and Methods: A total of 289 milk samples were collected from udder secretions of sheep (n=189) and goat (n=100) from El-Fayoum, Beni-Suef, and Giza governorates. Screening subclinical mastitis (SCM) was done using California Mastitis Test (CMT); identification of the isolates was achieved using Gram's staining, hemolytic pattern, colony morphology, and biochemical tests using Analytical Profile Index...
March 2018: Veterinary World
Quinn S McFrederick, Hoang Q Vuong, Jason A Rothman
Gram-stain-positive, rod-shaped, non-spore forming bacteria have been isolated from flowers and the guts of adult wild bees in the families Megachilidae and Halictidae. Phylogenetic analysis of the 16S rRNA gene indicated that these bacteria belong to the genus Lactobacillus, and are most closely related to the honey-bee associated bacteria Lactobacillus kunkeei (97.0 % sequence similarity) and Lactobacillus apinorum (97.0 % sequence similarity). Phylogenetic analyses of 16S rRNA genes and six single-copy protein coding genes, in situ and in silico DNA-DNA hybridization, and fatty-acid profiling differentiates the newly isolated bacteria as three novel Lactobacillus species: Lactobacillus micheneri sp...
April 12, 2018: International Journal of Systematic and Evolutionary Microbiology
Ram Hari Dahal, Jaisoo Kim
A novel bacterium, designated strain CA-1T , was isolated from forest soil in Kyonggi University. Cells were strictly aerobic, Gram-stain-negative, catalase-positive, oxidase-negative, non-motile, non-spore-forming, rod-shaped and red-orange-pigmented. Strain CA-1T hydrolysed casein and DNA. It was able to grow at 15-37 °C, pH 5.5-9.0 and at 0-2 % (w/v) NaCl concentration. Flexirubin-type pigments were present. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain CA-1T formed a lineage within the family Crocinitomicaceae of the phylum Bacteroidetes that was distinct from Fluviicola hefeinensis MYL-8T (96...
April 12, 2018: International Journal of Systematic and Evolutionary Microbiology
A Maleb, J Elmalki, O Bouayadi, Y Ben Lahlou, M Frikh, N Abdeljaouad, A Lemnouer, H Yacoubi, M Elouennass
Pasteurella multocida can cause serious infections after dog or cat bite. We report here a rare case of hand infection caused by P. multocida consecutive to an injury by a thorn of the prickly pear. It caused an amputation of the distal phalanx of the thumb in a trisomic patient. It is about a 27-year-old man who was admitted to the hospital with swelling and intense pain of the left hand. He reported a sting by a thorn of prickly pear 15 days before. The patient was admitted to proceed with operative irrigation and debridement...
February 2018: Trauma Case Reports
Norberto Gonzalez-Juarbe, Kelley M Bradley, Ashleigh N Riegler, Luis F Reyes, Terry Brissac, Sang-Sang Park, Marcos I Restrepo, Carlos J Orihuela
Pore-forming toxins are the most common virulence factor in pathogenic bacteria. They lead to membrane permeabilization and cell death. Herein, we show that respiratory epithelial cells (REC) undergoing bacterial pore-forming toxin (PFT)-induced necroptosis simultaneously experienced caspase activation independently of RIPK3. MLKL deficient REC treated with a pan-caspase inhibitor were protected in an additive manner against PFT-induced death. Subsequently, cleaved versions of caspases-2, -4 and -10 were detected within REC undergoing necroptosis by immunoblots and monoclonal antibody staining...
April 11, 2018: Scientific Reports
Jane R Schwebke, Charlotte A Gaydos, Paul Nyirjesy, Sonia Paradis, Salma Kodsi, Charles K Cooper
Background: Vaginitis is a common complaint, diagnosed either empirically or using Amsel's criteria and wet mount microscopy. Objective: To determine characteristics of an investigational test (a molecular-based test for vaginitis), as compared to reference, for detection of bacterial vaginosis, Candida spp., and Trichomonas vaginalis Study Design: Vaginal specimens from a cross-sectional study were obtained from 1,740 women (≥18 years old), with vaginitis symptoms, during routine clinic visits (across 10 sites in the USA)...
April 11, 2018: Journal of Clinical Microbiology
Heba Shaaban, David A Westfall, Rawhi Mohammad, David Danko, Daniela Bezdan, Ebrahim Afshinnekoo, Nicola Segata, Christopher E Mason
The Microbe Directory is a collective research effort to profile and annotate more than 7,500 unique microbial species from the MetaPhlAn2 database that includes bacteria, archaea, viruses, fungi, and protozoa. By collecting and summarizing data on various microbes' characteristics, the project comprises a database that can be used downstream of large-scale metagenomic taxonomic analyses, allowing one to interpret and explore their taxonomic classifications to have a deeper understanding of the microbial ecosystem they are studying...
January 5, 2018: Gates open research
Jing Hou, Yang-Jie Zhao, Lin Zhu, Heng-Lin Cui
A halophilic archaeal strain, designated ZS-35-S2T , was isolated from Zhoushan marine solar saltern in Zhejiang Province, China. Cells were pleomorphic, Gram-stain-negative and formed red-pigmented colonies on agar plates. The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). Strain ZS-35-S2T was able to grow at 25-50 °C (optimum, 37 °C), with 1.4-4.8 M NaCl (optimum, 2.1 M), with 0-1.0 M MgCl2 (optimum, 0.1 M) and at pH 5.0-9.5 (optimum, pH 7.5)...
April 9, 2018: International Journal of Systematic and Evolutionary Microbiology
Haiyuan Cai, Weihong He, Wang Yanan, Zaisheng Yan, Changhui Wang, Huacheng Xu, Keqiang Shao
A bacterial strain, designated TH167T , was isolated from cyanobacterial aggregates in eutrophic Lake Taihu in China. Cells were observed to be slightly curved rod-shaped, motile by gliding, aerobic, Gram-stain-negative, proteorhodopsin-containing. Optimal growth was obtained at pH 7.0 (range: 6.0-9.0), 28 °C (range: 4-32 °C) and 0 % (w/v) NaCl (range: 0-2.0 %) in Reasoner's 2A broth. No growth was observed at 37 °C. The cells were found to be positive for catalase and oxidase activities. The major fatty acids (>10 %) were identified as iso-C15 : 0, iso-C15 : 1 G and anteiso-C15 : 0...
April 9, 2018: International Journal of Systematic and Evolutionary Microbiology
Shunpei Horii, Hirotaka Yada, Kei Ito, Ayumu Osaki, Atsushi Sato, Toyokazu Kimura, Risako Yasuda, Takumi Toya, Takayuki Namba, Nobuyuki Masaki, Takeshi Adachi
Purulent pericarditis is a rare disease in the antibiotic era. The common pathogens of purulent pericarditis are gram-positive species such as Staphylococcus aureus. Streptococcus pneumoniae, Salmonella, Haemophilus, fungal pathogens/tuberculosis can also result in purulent pericarditis. We report an old male case of purulent pericarditis by Escherichia coli. He came to our hospital suffering from leg edema for 3 months. Echocardiography revealed the large amount of pericardial effusion, and he was admitted to test the cause of pericardial effusion without high fever, tachycardia, and shock vital signs...
April 6, 2018: International Heart Journal
Dhiraj Kumar Chaudhary, Jaisoo Kim
A novel strain K-4-16T was isolated from forest soil of Namsan Mountain, Seoul, South Korea, and was taxonomically characterized by a polyphasic approach. Strain K-4-16T was observed to be a Gram-staining negative, grayish white-coloured, motile with peritrichous flagella, and rod shaped bacterium. It was able to grow at 15-45 °C, at pH 4.5-10.5, and at 0-4% (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain K-4-16T belongs to the genus Acidovorax and is closely related to Acidovorax anthurii CFBP 3232T (98...
April 7, 2018: Antonie Van Leeuwenhoek
Dong-Jin Ko, Ji-Sun Kim, Doo-Sang Park, Dae-Hee Lee, Sun-Yeon Heo, Jeong-Woo Seo, Chul Ho Kim, Baek-Rock Oh
The translucent white-coloured, Gram-stain-negative, aerobic, non-motile, fusiform-shaped bacterium (designated strain SY72T ) was isolated from waste-activated sludge. Optimal growth occurred at 30-37 °C and pH 6.0-7.0. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the novel isolate belonged to the family Rhodobacteraceae of the class Alphaproteobacteria. Strain SY72T is closely related to Tabrizicola aquatica KCTC 23724T (97.8 % 16S rRNA gene sequence similarity) and Pseudorhodobacter aquaticus DC2N1-10T (96...
April 6, 2018: International Journal of Systematic and Evolutionary Microbiology
Asghar Marwat, Hassan Mehmood, Ali Hussain, Muzammil Khan, Asad Ullah, Medha Joshi
The relationship between reactive arthritis and enteric infections caused by Yersinia enterocolitica, Campylobacter jejuni , and Salmonella typhimurium is well documented. Clostridium difficile colitis is a less recognized cause of reactive arthritis. We present a case of a 58-year-old woman with Clostridium difficile colitis complicated by reactive arthritis. A 58-year-old woman with no significant past medical history presented to our hospital with complaints of nonbloody watery diarrhea, abdominal pain for the past 1 week, and right knee pain starting 1 day prior...
January 2018: Journal of Investigative Medicine High Impact Case Reports
Rachel Beekman, Jessica M Hu, Steven I Aronin, Maricar F Malinis
We report a case of a Puerto Rican male with advanced AIDS who presented with multiple falls and pancytopenia. Magnetic resonance imaging (MRI) of the brain, as initial workup, revealed 2 ring-enhancing brain lesions. Initial cerebrospinal fluid analysis revealed minimal cells, mildly elevated protein, and no organism seen on gram stain. Due to prohibitive thrombocytopenia, brain biopsy was deferred. He had neither clinical nor radiographic improvement despite empiric therapy for both toxoplasmosis and bacterial abscesses...
April 2018: Neurohospitalist
Masafumi Shimoda, Takeshi Saraya, Shota Yonetani, Koji Araki, Hajime Takizawa
In general, physicians believe that the presence of bacterial engulfment in white blood cells (WBCs) on Gram-stained sputum is a hallmark of lower respiratory infection. However, no studies have described the significance or diagnostic accuracy of engulfment in lower respiratory tract infections.We prospectively studied sputum samples by Gram staining (Favor method) for their quality and engulfment score in WBCs obtained from patients with respiratory symptoms at inpatient and outpatient settings at Kyorin University Hospital between December 2012 and April 2015...
April 2018: Medicine (Baltimore)
Lili Tang, Zenghu Zhang, Chao Zhou, Rong Cui, Yu Tian, Yongyu Zhang
A novel Gram-stain-negative, aerobic, non-flagellated, pink-pigmented and rod-shaped strain with gliding motility, designated strain CCMM001T , was isolated from a mixed culture of Synechococcus species PCC7002 and a natural bacterial community from a sample of offshore seawater from Qingdao, China, during September 2014. The strain contained bacteriochlorophyll a with a small peak at 802 nm and a large in vivo absorption band at 870 nm. Strain CCMM001T grew optimally at pH 7.0 and 30 °C in the presence of 3 % (w/v) NaCl...
April 5, 2018: International Journal of Systematic and Evolutionary Microbiology
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