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Gram's stain

Stefanie P Glaeser, Swapnil Doijad, Muaz Hijazin, Trinad Chakraborty, Enevold Falsen, Val Hall, Peter Kämpfer
During the years 1994-2011 five strictly anaerobic, Gram-stain-positive, diphtheroid bacteria (strains CCUG 31793(T), CCUG 44221, CCUG 61255, CCUG 45114, and CCUG 44993) were isolated from different clinical samples in Sweden and the United Kingdom. Comparative analysis of 16S rRNA gene sequences showed that the five strains shared 99-100% 16S rRNA gene sequence similarity among each other and 98.3-98.6% sequence similarity to Varibaculum cambriense DSM 15806(T). Genomic fingerprint patterns generated with ERIC-, BOX-, and RAPD-PCR, and whole genome sequence (WGS) based comparison by in silico DNA-DNA hybridization (isDDH), average nucleotide identity (ANI) analysis and six housekeeping gene (atpA, rpoB, pgi, metG, gltA and gyrA) based multilocus sequence analysis (MLSA) showed that the strains could be differentiated from V...
September 20, 2016: Systematic and Applied Microbiology
Long Jin, So-Ra Ko, Yingshun Cui, Chang Soo Lee, Hee-Mock Oh, Chi-Yong Ahn, Hyung-Gwan Lee
A polyphasic taxonomic study was carried out on strain EBR-8-1(T) isolated from a biofilm reactor in Korea. The cells of the strain were Gram-stain negative, non-spore-forming, non-motile, and short rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of this strain with Betaproteobacteria, which showed high pairwise sequence similarities with Pusillimonas noertemannii BN9(T) (99.1 %), Pusillimonas soli MJ07(T) (97.3 %), Pusillimonas ginsengisoli DCY25(T) (97.2 %), and Pusillimonas harenae B201(T) (96...
October 15, 2016: Antonie Van Leeuwenhoek
Emilio Bouza, José Martínez-Alarcón, Emilio Maseda, Mercedes Palomar, Rafael Zaragoza, María Jesús Pérez-Granda, Patricia Muñoz, Almudena Burillo
INTRODUCTION: Current guidelines for the microbiological diagnosis of ventilator-associated pneumonia (VAP) are imprecise. Based on data provided by intensive care specialists (ICS) and microbiologists, this study defines the clinical practices and microbiological techniques currently used for an aetiological diagnosis of VAP and pinpoints deficiencies. METHODS: Eighty hospitals in the national health network with intensive care and microbiology departments were sent two questionnaires, one for each department, in order to collect data on VAP diagnosis for the previous year...
October 13, 2016: Enfermedades Infecciosas y Microbiología Clínica
Mansour Amin, Mozhgan Ghaderpanah, Tahereh Navidifar
Meningitis is a life-threatening infection associated with a high mortality and morbidity worldwide. Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae are the most prevalent infectious agents that cause bacterial meningitis (BM). The objective of this study was to determine the frequencies of these three bacteria using bacterial cultures and polymerase chain reaction (PCR). In our cross-sectional study, cerebrospinal fluid (CSF) specimens were obtained from 196 patients who were suspected of having BM and referred to the pediatric ward of Abuzar Hospital (Ahvaz, Iran)...
October 2016: Kaohsiung Journal of Medical Sciences
Jun Zhang, Xiao-Tong Ma, Jun-Sheng Gao, Cai-Wen Zhang, Juan-Juan Zhao, Rui-Jie Zhang, Li-An Ma, Xiao-Xia Zhang
A novel bacterium, strain 1ZS3-15(T), was isolated from rhizosphere of rice. Its taxonomic position was investigated using a polyphasic approach. The novel strain was observed to be Gram-stain positive, spore-forming, aerobic, motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 1ZS3-15(T) was recovered within the genus Paenibacillus. It is closely related to Paenibacillus pectinilyticus KCTC 13222(T) (97.9 % similarity), Paenibacillus frigoriresistens CCTCC AB 2011150(T) (96...
October 12, 2016: Antonie Van Leeuwenhoek
Garrett L Metcalf, Scott R McClure, Jesse M Hostetter, Rudy F Martinez, Chong Wang
Use of mesenchymal stem cells (MSCs) found in the stromal vascular fraction (SVF) of equine adipose tissue has promising applications for regenerative therapies. The most commonly used source of equine adipose tissue is the subcutaneous tailhead. The objective of this study was to compare 3 adipose depot sites in horses and determine the viability and cellular yield, capillary density, gene expression for selected markers, and colony-forming unit fibroblasts (CFU-Fs) in adipose tissue taken from these sites...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
Henning Gram Hansen, Helene Faustrup Kildegaard, Gyun Min Lee, Stefan Stefan Kol
Accurate titer determination of recombinant proteins is crucial for evaluating protein production cell lines and processes. Even though enzyme-linked immunosorbent assay (ELISA) is the most widely used assay for determining protein titer, little is known about the accuracy of commercially available ELISA kits. We observed that estimations of recombinant human ø1-antitrypsin (rø1AT) titer by Coomassie-stained SDS-PAGE gels did not correspond to previously obtained titers obtained by a commercially available ELISA kit...
October 12, 2016: Biotechnology Journal
Arshia Amin, Iftikhar Ahmed, Nauman Khalid, Ghenijan Osman, Inam Ullah Khan, Min Xiao, Wen-Jun Li
A Gram-staining positive, non-motile, rod-shaped, catalase positive and oxidase negative bacterium, designated NCCP-1331(T), was isolated from a hot water spring soil collected from Tatta Pani, Kotli, Azad Jammu and Kashmir, Pakistan. The isolate grew at a temperature range of 18-40 °C (optimum 30 °C), pH 6.0-9.0 (optimum 7.0) and with 0-6 % NaCl (optimum 2 % NaCl (w/v)). The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain NCCP-1331(T) belonged to the genus Streptomyces and is closely related to Streptomyces brevispora BK160(T) with 97...
October 11, 2016: Antonie Van Leeuwenhoek
Menglan Zhou, Peng Wang, Sharon Chen, Bin Du, Jinlong Du, Fengdan Wang, Meng Xiao, Fanrong Kong, Yingchun Xu
BACKGROUND: Mycoplasma hominis, a well known cause of neonatal infection, has been reported as a pathogen in urogenital infections in adults; however, central nervous system (CNS) infections are rare. We report here the first case of M. hominis meningitis in China, post neurosurgical treatment for an intracerebral haemorrhage in a 71-year-old male. CASE PRESENTATION: We describe a 71-year-old man who developed M. hominis meningitis after neurosurgical treatment and was successfully treated with combined azithromycin and minocycline therapy of 2 weeks duration, despite delayed treatment because the Gram stain of cerebrospinal fluid (CSF) yielded no visible organisms...
October 12, 2016: BMC Infectious Diseases
Roni Bitterman, Mical Paul, Dina Polak, Yuval Geffen
No abstract text is available yet for this article.
October 5, 2016: Clinical Microbiology and Infection
Marquitta Smith, Thuvan Piehler, Richard Benjamin, Karen L Farizatto, Morgan C Pait, Michael F Almeida, Vladimir V Ghukasyan, Ben A Bahr
Explosives create shockwaves that cause blast-induced neurotrauma, one of the most common types of traumatic brain injury (TBI) linked to military service. Blast-induced TBIs are often associated with reduced cognitive and behavioral functions due to a variety of factors. To study the direct effects of military explosive blasts on brain tissue, we removed systemic factors by utilizing rat hippocampal slice cultures. The long-term slice cultures were briefly sealed air-tight in serum-free medium, lowered into a 37°C water-filled tank, and small 1...
October 5, 2016: Experimental Neurology
X Lyu, C Zhao, Z M Yan, H Hua
Objective: To explore a simple, rapid and efficient method for the diagnosis of oral candidiasis in clinical practice. Methods: Totally 124 consecutive patients with suspected oral candidiasis were enrolled from Department of Oral Medicine, Peking University School and Hospital of Stomatology, Beijing, China. Exfoliated cells of oral mucosa and saliva or concentrated oral rinse) obtained from all participants were tested by three rapid smear methods(10% KOH smear, gram-stained smear, Congo red stained smear)...
October 9, 2016: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
Linoj Samuel, Mario Plebani
No abstract text is available yet for this article.
October 8, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
Xueli Sun, Zhiyong Qian, Lingqiong Luo, Qipeng Yuan, Ximin Guo, Lei Tao, Yen Wei, Xing Wang
Polymethyl methacrylate (PMMA) is a widely used biomaterial. But there is still a challenge facing its unwanted bacterial adhesion, because the subsequent biofilm formation usually leads to failure of related implants. Herein, we present a borneol-modified PMMA based on a facile and effective stereochemical strategy, generating antibacterial copolymer named as P(MMA-co-BA). It was synthesized by free radical polymerization and studied with different ratio between methyl methacrylate (MMA) and borneol acrylate (BA) monomers...
October 7, 2016: ACS Applied Materials & Interfaces
Matthew S Delfiner, Luis R Martinez, Charles S Pavia
BACKGROUND: Laboratory diagnostic tests have an essential role in patient care, and the increasing number of medical and health professions schools focusing on teaching laboratory medicine to pre-clinical students reflects this importance. However, data validating the pedagogical methods that best influence students' comprehension and interpretation of diagnostic tests have not been well described. The Gram stain is a simple yet significant and frequently used diagnostic test in the clinical setting that helps classify bacteria into two major groups, Gram positive and negative, based on their cell wall structure...
2016: PloS One
Luis Franca, Luciana Albuquerque, De-Chao Zhang, Imen Nouioui, Hans-Peter Klenk, Milton S da Costa, Rosa Margesin
A positive Gram-staining, non-motile, catalase-positive and cytochrome c oxidase-negative bacterium, designated strain S20-107T, was isolated from alpine forest soil. Growth occurred at a temperature range of 0-30 °C, at pH 6-9 and in the presence of 0-3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-107T was related to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity with the type strain of Nakamurella flavida DS-52T (96.1 %). Strain S20-107T showed <96...
October 4, 2016: International Journal of Systematic and Evolutionary Microbiology
Sooyeon Park, Min-Ji Ha, Sun Young Yoon, Yong-Taek Jung, Jung-Hoon Yoon
A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated strain OITF-19T, was isolated from a tidal flat in oido, an island of South Korea, and subjected to polyphasic taxonomic study. Strain OITF-19T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain OITF-19T belonged to the genus Algoriphagus, clustering with the type strain of A. namhaensis showing 96...
September 30, 2016: International Journal of Systematic and Evolutionary Microbiology
Ashish Verma, Anup Kumar Ojha, Parveen Mual, Shiva S Sundharam, Shanmugam Mayilraj, Srinivasan Krishnamurthi
A Gram-staining-negative, rod shaped bacterium forming yellow coloured colonies was isolated from the surface of Padina sp., a brown macroalgae occurring in the Western coastal regions of the state of Goa, India and was designated as CDR SL 15T. The 16S rRNA gene sequence phylogeny placed the strain in the genus Luteimonas and showed closest sequence similarity to Luteimonas terricola BZ92rT (97.6%) and <97.0% to other Luteimonas spp. Chemotaxonomic features like iso-C15:0 and summed feature 9 (C16:0 10-methyl/ iso-C17:1 ω9c) as the major fatty acids and Q-8 as the only ubiquinone further supported its placement within the genus...
October 4, 2016: International Journal of Systematic and Evolutionary Microbiology
Erik R Dubberke, Kathleen M Mullane, Dale N Gerding, Christine H Lee, Thomas J Louie, Harriet Guthertz, Courtney Jones
Background.  Vancomycin-resistant Enterococcus (VRE) is a major healthcare-associated pathogen and a well known complication among transplant and immunocompromised patients. We report on stool VRE clearance in a post hoc analysis of the Phase 2 PUNCH CD study assessing a microbiota-based drug for recurrent Clostridium difficile infection (CDI). Methods.  A total of 34 patients enrolled in the PUNCH CD study received 1 or 2 doses of RBX2660 (microbiota suspension). Patients were requested to voluntarily submit stool samples at baseline and at 7, 30, and 60 days and 6 months after the last administration of RBX2660...
September 2016: Open Forum Infectious Diseases
Stefan Wiehr, Anna-Maria Rolle, Philipp Warnke, Ursula Kohlhofer, Leticia Quintanilla-Martinez, Gerald Reischl, Ingo B Autenrieth, Bernd J Pichler, Stella E Autenrieth
Most frequently, gram-negative bacterial infections in humans are caused by Enterobacteriaceae and remain a major challenge in medical diagnostics. We non-invasively imaged moderate and severe systemic Yersinia enterocolitica infections in mice using the positron emission tomography (PET) tracer 3'-deoxy-3'-[18F]fluorothymidine ([18F]FLT), which is a marker of proliferation, and compared the in vivo results to the ex vivo biodistributions, bacterial loads, and histologies of the corresponding organs. Y. enterocolitica infection is detectable with histology using H&E staining and immunohistochemistry for Ki 67...
2016: PloS One
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