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https://www.readbyqxmd.com/read/28706950/effects-of-cobalt-chloride-a-hypoxia-mimetic-agent-on-autophagy-and-atrophy-in-skeletal-c2c12-myotubes
#1
Rui Chen, Ting Jiang, Yanling She, Jiehua Xu, Cheng Li, Shanyao Zhou, Huijuan Shen, Huacai Shi, Shuang Liu
BACKGROUND: Hypoxia-induced autophagy and muscle wasting occur in several environmental and pathological conditions. However, the molecular mechanisms underlying the effects of the hypoxia-mimetic agent CoCl2 on autophagy and muscle atrophy are still unclear. METHODS: C2C12 myotubes were exposed to increasing concentrations of CoCl2 for 24 hours. Quantitative RT-PCR, Western blotting, and transmission electron microscopy were performed to confirm autophagy occurs...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28705993/effects-of-%C3%AE-hydroxy-%C3%AE-methylbutyrate-hmb-on-skeletal-muscle-mitochondrial-content-and-dynamics-and-lipids-after-10-days-of-bed-rest-in-older-adults
#2
Robert A Standley, Giovanna Distefano, Suzette L Pereira, Min Tian, Owen J Kelly, Paul M Coen, Nicolaas E P Deutz, Robert R Wolfe, Bret H Goodpaster
Loss of muscle mass during periods of disuse likely has negative health consequences for older adults. We have previously shown that β-hydroxy-β-methylbutyrate (HMB) supplementation during 10 days of strict bed rest (BR) attenuates the loss of lean mass in older adults. To elucidate potential molecular mechanisms of HMB effects on muscle during bed rest and resistance training rehabilitation (RT), we examined mediators of skeletal muscle mitochondrial dynamics, autophagy and atrophy, and intramyocellular lipids...
July 13, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28705935/forkhead-box-o3-foxo3-regulates-kidney-tubular-autophagy-following-urinary-tract-obstruction
#3
Ling Li, Ronald Zviti, Catherine Ha, Zhao V Wang, Joseph A Hill, Fangming Lin
Autophagy has been shown to be important for normal homeostasis and adaptation to stress in the kidney. Yet, the molecular mechanisms regulating renal epithelial autophagy are not fully understood. Here, we explored the role of the stress-responsive transcription factor forkhead box O3 (FoxO3) in mediating injury-induced proximal tubular autophagy in mice with unilateral ureteral obstruction (UUO). We show that following UUO, FoxO3 is activated and displays nuclear expression in the hypoxic proximal tubules exhibiting high levels of autophagy...
July 13, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28704726/bnip3-induction-by-hypoxia-stimulates-fasn-dependent-free-fatty-acid-production-enhancing-therapeutic-potential-of-umbilical-cord-blood-derived-human-mesenchymal-stem-cells
#4
Hyun Jik Lee, Young Hyun Jung, Gee Euhn Choi, So Hee Ko, Sei-Jung Lee, Sang Hun Lee, Ho Jae Han
Mitophagy under hypoxia is an important factor for maintaining and regulating stem cell functions. We previously demonstrated that fatty acid synthase (FASN) induced by hypoxia is a critical lipid metabolic factor determining the therapeutic efficacy of umbilical cord blood-derived human mesenchymal stem cells (UCB-hMSCs). Therefore, we investigated the mechanism of a major mitophagy regulator controlling lipid metabolism and therapeutic potential of UCB-hMSCs. This study revealed that Bcl2/adenovirus E1B 19kDa protein-interacting protein 3 (BNIP3)-dependent mitophagy is important for reducing mitochondrial reactive oxygen species accumulation, anti-apoptosis, and migration under hypoxia...
July 4, 2017: Redox Biology
https://www.readbyqxmd.com/read/28698145/increase-in-proteins-involved-in-mitochondrial-fission-mitophagy-proteolysis-and-antioxidant-response-in-type-i-endometrial-cancer-as-an-adaptive-response-to-respiratory-complex-i-deficiency
#5
Antonella Cormio, Clara Musicco, Giuseppe Gasparre, Gennaro Cormio, Vito Pesce, AnnaMaria Sardanelli, Maria Nicola Gadaleta
Pathogenic mtDNA mutations associated with alterations of respiratory complex I, mitochondrial proliferation (oncocytic-like phenotype) and increase in antioxidant response were previously reported in type I endometrial carcinoma (EC). To evaluate whether in the presence of pathogenic mtDNA mutations other mitochondrial adaptive processes were triggered by cancer cells, the expression level of proteins involved in mitochondrial dynamics, mitophagy, proteolysis and apoptosis were evaluated in type I ECs harboring pathogenic mtDNA mutations and complex I deficiency...
July 8, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28696827/isolation-of-rab5-positive-endosomes-reveals-a-new-mitochondrial-degradation-pathway-utilized-by-bnip3-and-parkin
#6
Babette C Hammerling, Sarah E Shires, Leonardo J Leon, Melissa Q Cortez, Åsa B Gustafsson
Degradation of mitochondria is an important cellular quality control mechanism mediated by two distinct pathways: one involving Parkin-mediated ubiquitination and the other dependent on mitophagy receptors. It is known that mitochondria are degraded by the autophagy pathway; however, we recently reported that the small GTPase Rab5 and early endosomes also participate in Parkin-mediated mitochondrial clearance. Here, we have developed a protocol to isolate Rab5-positive vesicles from cells for proteomics analysis and provide additional data confirming that mitophagy regulators and mitochondrial proteins are present in these vesicles...
July 11, 2017: Small GTPases
https://www.readbyqxmd.com/read/28687017/methylation-of-bnip3-in-pancreatic-cancer-inhibits-the-induction-of-mitochondrial-mediated-tumor-cell-apoptosis
#7
Ye Li, Xu Zhang, Jian Yang, Yi Zhang, Dongming Zhu, Lifeng Zhang, Yanbo Zhu, Dechun Li, Jian Zhou
Bcl-2 interacting protein 3 (BNIP3) is involved in various cellular processes and is considered a key regulator of hypoxia-induced apoptosis. In the present study, the expression of BNIP3 in pancreatic cancer tissues, the correlation with clinicopathological characteristics and prognosis and the regulation of this protein in pancreatic cancer cell lines with regard to the induction of apoptosis were investigated. BNIP3 expression was significantly lower in pancreatic cancer tissues compared with normal epithelia and was associated with tumor size, clinical stage, and lymph node metastasis...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28681509/akt2-ablation-prolongs-life-span-and-improves-myocardial-contractile-function-with-adaptive-cardiac-remodeling-role-of-sirt1-mediated-autophagy-regulation
#8
Jun Ren, Lifang Yang, Li Zhu, Xihui Xu, Asli F Ceylan, Wei Guo, Jian Yang, Yingmei Zhang
Aging is accompanied with unfavorable geometric and functional changes in the heart involving dysregulation of Akt and autophagy. This study examined the impact of Akt2 ablation on life span and cardiac aging as well as the mechanisms involved with a focus on autophagy and mitochondrial integrity. Cardiac geometry, contractile, and intracellular Ca(2+) properties were evaluated using echocardiography, IonOptix(®) edge-detection and fura-2 techniques. Levels of Sirt1, mitochondrial integrity, autophagy, and mitophagy markers were evaluated using Western blot...
July 5, 2017: Aging Cell
https://www.readbyqxmd.com/read/28661226/microrna-210-alleviates-oxidative-stress-associated-cardiomyocyte-apoptosis-by-regulating-bnip3
#9
Hongying Diao, Bin Liu, Yongfeng Shi, Chunli Song, Ziyuan Guo, Ning Liu, Xianjing Song, Yang Lu, Xiaoye Lin, Zhuoran Li
Oxidative stress-induced myocardial apoptosis and necrosis are involved in ischemia/reperfusion (I/R) injury. This study was performed to investigate microRNA (miR)-210's role in oxidative stress-related myocardial damage. The expression of miR-210 was upregulated in myocardial tissues of I/R rats, while that of Bcl-2 adenovirus E1B 19kDa-interacting protein 3 (BNIP3) was downregulated. To simulate in vivo oxidative stress, H9c2 cells were treated with H2O2 for 48 h. MiR-210 level was increased upon H2O2 stimulation, peaked at 8 h, and then decreased...
June 29, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28659735/pulmonary-pseudomonas-aeruginosa-infection-induces-autophagy-and-proteasome-proteolytic-pathways-in-skeletal-muscles-effects-of-a-pressurized-whey-protein-based-diet-in-mice
#10
Osama A Kishta, Yeting Guo, Mahroo Mofarrahi, Flavia Stana, Larry C Lands, Sabah N A Hussain
Background: Pulmonary Pseudomonas aeruginosa infection in cystic fibrosis patients is associated with skeletal muscle atrophy. In this study, we investigated the effects of P. aeurginosa infection and a whey protein-rich diet on skeletal muscle proteolytic pathways. Design: An agar bead model of pulmonary P. aeurginosa infection was established in adult C57/Bl6 mice. Protein ubiquitinaiton, lipidation of LC3B protein and expressions of autophagy-related genes and ubiquitin E3 ligases were quantified using immunoblotting and qPCR...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28658662/microrna-145-induces-apoptosis-of-glioma-cells-by-targeting-bnip3-and-notch-signaling
#11
Yan Du, Juan Li, Tao Xu, Dan-Dan Zhou, Lei Zhang, Xiao Wang
MicroRNAs (miRNAs) are involved in the pathogenesis of various human cancers. Here we show that miR-145 expression is decreased in human glioma samples, rat glioma tissues, and glioma cell lines, while expression of BNIP3 is increased. Over-expression of miR-145 or suppression of BNIP3 induced glioma cell apoptosis. BNIP3 is localized in the nucleus in glioma cells, and miR-145 inhibits BNIP3 expression by binding to the 3' untranslated region of its mRNA. Interestingly, miR-145 and BNIP3 regulate glioma cell apoptosis by modulating Notch signaling...
June 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28653874/invalidation-of-mitophagy-by-fbp1-mediated-repression-promotes-apoptosis-in-breast-cancer
#12
Yifeng Liu, Yulin Jiang, Nian Wang, Qianni Jin, Feihu Ji, Changli Zhong, Zhiqiang Zhang, Junhong Yang, Xiangsen Ye, Tingmei Chen
Fructose-1,6-bisphosphatase 1, a rate-limiting enzyme in gluconeogenesis, was recently shown to be a tumor suppressor. However, the functions of fructose-1,6-bisphosphatase 1 in the regulation of mitophagy and apoptosis remain unknown. Here, we investigated the effects of fructose-1,6-bisphosphatase 1 on mitophagy and apoptosis as well as their underlying mechanisms in breast cancer cells. In this work, the messenger RNA and protein expression of various molecules were determined by quantitative realtime polymerase chain reaction and western blot, respectively...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28619984/mitochondrial-integrity-and-function-in-the-progression-of-early-pressure-overload-induced-left-ventricular-remodeling
#13
Antoine H Chaanine, K Sreekumaran Nair, Robert H Bergen, Katherine Klaus, Adam J Guenzel, Roger J Hajjar, Margaret M Redfield
BACKGROUND: Following pressure overload, compensatory concentric left ventricular remodeling (CR) variably transitions to eccentric remodeling (ER) and systolic dysfunction. Mechanisms responsible for this transition are incompletely understood. Here we leverage phenotypic variability in pressure overload-induced cardiac remodeling to test the hypothesis that altered mitochondrial homeostasis and calcium handling occur early in the transition from CR to ER, before overt systolic dysfunction...
June 15, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28618048/transcriptomic-profiling-in-human-decidua-of-severe-preeclampsia-detected-by-rna-sequencing
#14
Jing Tong, Weixiu Zhao, Hong Lv, Wei-Ping Li, Zi-Jiang Chen, Cong Zhang
Maternal decidua plays a critical role in implantation and placentation. Impaired decidualization causes failed intravascular trophoblast invasion and inadequate placentation and then increases the risk of preeclampsia (PE). RNA sequencing (RNA-Seq) has achieved great advances in the characterization and quantification of transcriptomes, is a powerful tool for new transcript discovery, genome annotation and expression profiling. In the present study, we conducted a RNA-Seq analysis to compare gene expression between decidua of PE (n = 3, early-onset severe PE, EOSPE; n = 3, late-onset severe PE, LOSPE) and normal pregnancies (n = 3)...
June 15, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28616911/-histone-deacetylation-down-regulates-the-expression-of-bnip3-in-renal-cell-carcinoma
#15
Jian-Bang Liu, Hai-Zhou Wang, Zhen-Hua Liu, Miao Xu, Xiang Li
OBJECTIVES: To investigate the down-regulation mechanism of (bcl-2/adenovirus E1B 19 kDa interacting protein 3 (BNIP3) expression in renal cell carcinoma (RCC). METHODS: RCC cell lines 786-O, ACHN and A498 were treated with different concentrations of histone deacetylase inhibitor TSA. Thereafter, the proliferation of RCC cells was determined with CCK-8 assay, cell apoptosis was observed by flow cytometry, and the expression levels of BNIP3 were determined by Q-PCR and Western blot, and the acetylation status of histone H3 in the promoter of BNIP3 was detected by ChIP...
May 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28607593/chemoresistance-of-colorectal-cancer-to-5-fluorouracil-is-associated-with-silencing-of-the-bnip3-gene-through-aberrant-methylation
#16
Jianming He, Li Pei, Heng Jiang, Weiwen Yang, Jianfang Chen, Houjie Liang
Purpose To investigate the correlation between chemoresistance of colorectal cancer to 5-fluorouracil and BNIP3 and the underlying mechanism. Methods BNIP3 protein in specimens was evaluated using immunohistochemistry. Semi-quantitative reverse transcription PCR and Western blot was employed to assay gene expression. The promoter methylation status of BNIP3 was examined by methylation-specific PCR. Drug sensitivity was assayed using MTT assay. Results Specimens from 81 patients with colorectal cancer receiving 5-fluorouracil-based chemotherapy were analyzed...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28607206/downregulation-of-mir-199a-5p-promotes-prostate-adeno-carcinoma-progression-through-loss-of-its-inhibition-of-hif-1%C3%AE
#17
Jinjing Zhong, Rui Huang, Zhengzheng Su, Mengni Zhang, Miao Xu, Jing Gong, Ni Chen, Hao Zeng, Xueqin Chen, Qiao Zhou
Hypoxia-inducible factor-1 alpha (HIF-1α) plays key roles in cell survival under both hypoxia and normoxia conditions. Regulation of HIF-1α is complex and involves numerous molecules and pathways, including post-transcriptional regulation by microRNAs (miRNAs). Although upregulation of HIF-1α has been shown to promote prostate adenocarcinoma (PCa) progression, the mechanism by which miRNAs modulate HIF-1α in prostate cancer has not been clarified. Here, we show that miR-199a-5p is underexpressed in prostate adenocarcinoma...
May 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/28586054/propofol-reduces-hypoxia%C3%A2-induced-autophagic-cell-death-through-downregulating-hif-1%C3%AE-in-alveolar-epithelial-type-ii-cells-of-rats
#18
Hui-Jie Ning, Hong-Bin Yuan, Hai-Tao Xu, Xing-Ying He
Propofol (2,6‑diisopropylphenol) exerts protective effects on alveolar epithelial type II (ATII) cells, partly through attenuating hypoxia‑induced apoptosis. Autophagy is involved in the activation of apoptosis. Therefore, the present study investigated the modulating effect of propofol against autophagy in ATII cells under hypoxia. Western blot analysis was performed to detect the protein expression of the autophagy molecular marker, microtubule‑associated protein 1 light chain 3 (LC3)‑II, under various conditions...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28570828/the-role-of-androgens-in-the-regulation-of-muscle-oxidative-capacity-following-aerobic-exercise-training
#19
Michael L Rossetti, Bradley S Gordon
Reduced production or bioavailability of androgens, termed hypogonadism, occurs in a variety of pathological conditions. While androgens target numerous tissues throughout the body, hypogonadism specifically reduces the ability of skeletal muscle to produce ATP aerobically i.e. muscle oxidative capacity. This has important implications for overall health as muscle oxidative capacity impacts a number of metabolic processes. Although androgen replacement therapy is effective at restoring muscle oxidative capacity in hypogonadal individuals, this is not a viable therapeutic option for all who are experiencing hypogonadism...
June 1, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28560697/the-cardiotoxic-mechanism-of-doxorubicin-dox-and-pegylated-liposomal-dox-in-mice-bearing-c-26-colon-carcinoma-a-study-focused-on-microrna-role-for-toxicity-assessment-of-new-formulations
#20
Kamal Razavi-Azarkhiavi, Mahmoud Reza Jaafari, Khalil Abnous, Bibi Marjan Razavi, Amir Hossein Jafarian, Faezeh Vahdati Hassani, Kobra Shirani, Gholamreza Karimi
PURPOSE: MicroRNAs (miRs) are a group of small non-coding RNAs that regulate transcriptional or post-transcriptional gene expression. The aim of the present study was to investigate the role of miR -1, -21 and -145 and their targets in cardiotoxicity-induced by DOX and pegylated liposomal DOX. METHODS: BALB/c mice subjected to subcutaneous injection of C-26 tumor cells. Eight days after tumor inoculation, animals were divided into 6 groups: control, liposome, DOX (6 and 9 mg/kg) and PL-DOX (6 and 9 mg/kg)...
May 30, 2017: Pharmaceutical Research
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