keyword
MENU ▼
Read by QxMD icon Read
search

Biophysical profile

keyword
https://www.readbyqxmd.com/read/28628484/battling-bacterial-infection-with-hexamethylene-diisocyanate-cross-linked-and-cefaclor-loaded-collagen-scaffolds
#1
E K Tsekoura, A L Helling, J G Wall, Y Bayon, D I Zeugolis
Implant infections remain a major healthcare problem due to the prolonged hospitalisation period required to disrupt and treat bacterial biofilm formation, and the need for additional surgery to remove/replace the infected implant, which if not removed in a timely manner may lead to sepsis. Although localised drug administration, via an implanted scaffold, has shown promise in a clinical setting, the ideal scaffold cross-linking (to initially withstand the aggressive infection environment) and drug (to be effective against infection) have yet to be identified...
June 19, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28614751/molecular-spectroscopic-and-thermodynamic-studies-on-the-interaction-of-anti-platelet-drug-ticlopidine-with-calf-thymus-dna
#2
Shumaila Afrin, Yusra Rahman, Tarique Sarwar, Mohammed Amir Husain, Abad Ali, Shamsuzzaman, Mohammad Tabish
Ticlopidine is an anti-platelet drug which belongs to the thienopyridine structural family and exerts its effect by functioning as an ADP receptor inhibitor. Ticlopidine inhibits the expression of TarO gene in S. aureus and may provide protection against MRSA. Groove binding agents are known to disrupt the transcription factor DNA complex and consequently inhibit gene expression. Understanding the mechanism of interaction of ticlopidine with DNA can prove useful in the development of a rational drug designing system...
June 11, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28612046/shaping-of-signal-transmission-at-the-photoreceptor-synapse-by-eaat2-glutamate-transporters
#3
Stephanie Niklaus, Lucia Cadetti, Colette M Vom Berg-Maurer, André Lehnherr, Adriana L Hotz, Ian C Forster, Matthias Gesemann, Stephan C F Neuhauss
Photoreceptor ribbon synapses tonically release glutamate. To ensure efficient signal transmission and prevent glutamate toxicity, a highly efficient glutamate removal system provided by members of the SLC1 gene family is required. By using a combination of biophysical and in vivo studies, we elucidate the role of excitatory amino acid transporter 2 (EAAT2) proteins in synaptic glutamate homeostasis at the zebrafish photoreceptor synapse. The main glutamate sink is provided by the glial EAAT2a, reflected by reduced electroretinographic responses in EAAT2a-depleted larvae...
May 2017: ENeuro
https://www.readbyqxmd.com/read/28611596/ethidium-bromide-modifies-the-agarose-electrophoretic-mobility-of-cag%C3%A2-ctg-alternative-dna-structures-generated-by-pcr
#4
Mário Gomes-Pereira, Darren G Monckton
The abnormal expansion of unstable simple sequence DNA repeats can cause human disease through a variety of mechanisms, including gene loss-of-function, toxic gain-of-function of the encoded protein and toxicity of the repeat-containing RNA transcript. Disease-associated unstable DNA repeats display unusual biophysical properties, including the ability to adopt non-B-DNA structures. CAG•CTG trinucleotide sequences, in particular, have been most extensively studied and they can fold into slipped-stranded DNA structures, which have been proposed as mutation intermediates in repeat size expansion...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28576704/%C3%AE-synuclein-fibril-induced-inclusion-spread-in-rats-and-mice-correlates-with-dopaminergic-neurodegeneration
#5
Hisham Abdelmotilib, Tyler Maltbie, Vedad Delic, Zhiyong Liu, Xianzhen Hu, Kyle B Fraser, Mark S Moehle, Lindsay Stoyka, Nadia Anabtawi, Valentina Krendelchtchikova, Laura A Volpicelli-Daley, Andrew West
Proteinaceous inclusions in neurons, composed primarily of α-synuclein, define the pathology in several neurodegenerative disorders. Neurons can internalize α-synuclein fibrils that can seed new inclusions from endogenously expressed α-synuclein. The factors contributing to the spread of pathology and subsequent neurodegeneration are not fully understood, and different compositions and concentrations of fibrils have been used in different hosts. Here, we systematically vary the concentration and length of well-characterized α-synuclein fibrils and determine their relative ability to induce inclusions and neurodegeneration in different hosts (primary neurons, C57BL/6J and C3H/HeJ mice, and Sprague Dawley rats)...
May 30, 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/28574984/complexity-in-action-untangling-latent-relationships-between-land-quality-economic-structures-and-socio-spatial-patterns-in-italy
#6
Luca Salvati, Ilaria Tombolini, Roberta Gemmiti, Margherita Carlucci, Sofia Bajocco, Luigi Perini, Agostino Ferrara, Andrea Colantoni
Land quality, a key economic capital supporting local development, is affected by biophysical and anthropogenic factors. Taken as a relevant attribute of economic systems, land quality has shaped the territorial organization of any given region influencing localization of agriculture, industry and settlements. In regions with long-established human-landscape interactions, such as the Mediterranean basin, land quality has determined social disparities and polarization in the use of land, reflecting the action of geographical gradients based on elevation and population density...
2017: PloS One
https://www.readbyqxmd.com/read/28569367/resonance-modulation-annihilation-and-generation-of-anti-resonance-and-anti-phasonance-in-3d-neuronal-systems-interplay-of-resonant-and-amplifying-currents-with-slow-dynamics
#7
Horacio G Rotstein
Subthreshold (membrane potential) resonance and phasonance (preferred amplitude and zero-phase responses to oscillatory inputs) in single neurons arise from the interaction between positive and negative feedback effects provided by relatively fast amplifying currents and slower resonant currents. In 2D neuronal systems, amplifying currents are required to be slave to voltage (instantaneously fast) for these phenomena to occur. In higher dimensional systems, additional currents operating at various effective time scales may modulate and annihilate existing resonances and generate antiresonance (minimum amplitude response) and antiphasonance (zero-phase response with phase monotonic properties opposite to phasonance)...
May 31, 2017: Journal of Computational Neuroscience
https://www.readbyqxmd.com/read/28555046/collagen-from-the-marine-sponges-axinella-cannabina-and-suberites-carnosus-isolation-and-morphological-biochemical-and-biophysical-characterization
#8
Leto-Aikaterini Tziveleka, Efstathia Ioannou, Dimitris Tsiourvas, Panagiotis Berillis, Evangelia Foufa, Vassilios Roussis
In search of alternative and safer sources of collagen for biomedical applications, the marine demosponges Axinella cannabina and Suberites carnosus, collected from the Aegean and the Ionian Seas, respectively, were comparatively studied for their insoluble collagen, intercellular collagen, and spongin-like collagen content. The isolated collagenous materials were morphologically, physicochemically, and biophysically characterized. Using scanning electron microscopy and transmission electron microscopy the fibrous morphology of the isolated collagens was confirmed, whereas the amino acid analysis, in conjunction with infrared spectroscopy studies, verified the characteristic for the collagen amino acid profile and its secondary structure...
May 29, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28554525/coupling-between-chromosome-intermingling-and-gene-regulation-during-cellular-differentiation
#9
REVIEW
Yejun Wang, Nikhil Jain, Mallika Nagarajan, Shovamayee Maharana, K Venkatesan Iyer, Shefali Talwar, G V Shivashankar
In this article, we summarize current findings for the emergence of biophysical properties such as nuclear stiffness, chromatin compaction, chromosome positioning, and chromosome intermingling during stem cell differentiation, which eventually correlated with the changes of gene expression profiles during cellular differentiation. An overview is first provided to link stem cell differentiation with alterations in nuclear architecture, chromatin compaction, along with nuclear and chromatin dynamics. Further, we highlight the recent biophysical and molecular approaches, imaging methods and computational developments in characterizing transcription-related chromosome organization especially chromosome intermingling and nano-scale chromosomal contacts...
May 26, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28554507/track-structure-model-of-microscopic-energy-deposition-by-protons-and-heavy-ions-in-segments-of-neuronal-cell-dendrites-represented-by-cylinders-or-spheres
#10
Murat Alp, Francis A Cucinotta
Changes to cognition, including memory, following radiation exposure are a concern for cosmic ray exposures to astronauts and in Hadron therapy with proton and heavy ion beams. The purpose of the present work is to develop computational methods to evaluate microscopic energy deposition (ED) in volumes representative of neuron cell structures, including segments of dendrites and spines, using a stochastic track structure model. A challenge for biophysical models of neuronal damage is the large sizes (> 100µm) and variability in volumes of possible dendritic segments and pre-synaptic elements (spines and filopodia)...
May 2017: Life Sciences in Space Research
https://www.readbyqxmd.com/read/28546283/high-resolution-mapping-of-gastric-slow-wave-recovery-profiles-biophysical-model-methodology-and-demonstration-of-applications
#11
Niranchan Paskaranandavadivel, Leo K Cheng, Peng Du, Jack M Rogers, Gregory O'Grady
Slow waves play a central role in coordinating gastric motor activity. High-resolution mapping of extracellular potentials from the stomach provides spatiotemporal detail on normal and dysrhythmic slow-wave patterns. All mapping studies to date have focused exclusively on tissue activation; however, the recovery phase contains vital information on repolarization heterogeneity, the excitable gap, and refractory tail interactions, but has not been investigated. Here, we report a method to identify the recovery phase in slow-wave mapping data...
May 25, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/28545134/structures-of-the-inactive-and-active-states-of-rip2-kinase-inform-on-the-mechanism-of-activation
#12
Erika Pellegrini, Luca Signor, Saurabh Singh, Elisabetta Boeri Erba, Stephen Cusack
Innate immune receptors NOD1 and NOD2 are activated by bacterial peptidoglycans leading to recruitment of adaptor kinase RIP2, which, upon phosphorylation and ubiquitination, becomes a scaffold for downstream effectors. The kinase domain (RIP2K) is a pharmaceutical target for inflammatory diseases caused by aberrant NOD2-RIP2 signalling. Although structures of active RIP2K in complex with inhibitors have been reported, the mechanism of RIP2K activation remains to be elucidated. Here we analyse RIP2K activation by combining crystal structures of the active and inactive states with mass spectrometric characterization of their phosphorylation profiles...
2017: PloS One
https://www.readbyqxmd.com/read/28543540/computational-modeling-of-915-mhz-microwave-ablation-comparative-assessment-of-temperature-dependent-tissue-dielectric-models
#13
Garron Deshazer, Mark Hagmann, Derek Merck, Jan Sebek, Kent B Moore, Punit Prakash
PURPOSE: The objective of this study is to develop a computational model for simulating 915 MHz microwave ablation (MWA), and verify the simulation predictions of transient temperature profiles against experimental measurements. Due to the limited experimental data characterizing temperature-dependent changes of tissue dielectric properties at 915 MHz, we comparatively assess two temperature dependent approaches of modeling of dielectric properties: model A- piecewise linear temperature dependencies based on existing, but limited, experimental data, and model B- similar to model A, but augmented with linear decrease in electrical conductivity above 95 °C, as guided by our experimental measurements...
May 23, 2017: Medical Physics
https://www.readbyqxmd.com/read/28528360/a-spiking-neural-network-model-of-the-midbrain-superior-colliculus-that-generates-saccadic-motor-commands
#14
Bahadir Kasap, A John van Opstal
Single-unit recordings suggest that the midbrain superior colliculus (SC) acts as an optimal controller for saccadic gaze shifts. The SC is proposed to be the site within the visuomotor system where the nonlinear spatial-to-temporal transformation is carried out: the population encodes the intended saccade vector by its location in the motor map (spatial), and its trajectory and velocity by the distribution of firing rates (temporal). The neurons' burst profiles vary systematically with their anatomical positions and intended saccade vectors, to account for the nonlinear main-sequence kinematics of saccades...
May 20, 2017: Biological Cybernetics
https://www.readbyqxmd.com/read/28501599/ph-induced-single-step-shift-of-hydrophobic-patches-followed-by-formation-of-an-mg-state-and-an-amyloidogenic-intermediate-in-lima-bean-trypsin-inhibitor-lbti
#15
Javed Masood Khan, Mohammad A Alsenaidy, Mohd Shahnawaz Khan, Priyankar Sen, Rizwan Hasan Khan, Sadaf Fatima
Lima Bean Trypsin Inhibitor (LBTI) is 83 residues monomeric protein of 9.0 KDa, consisting of six antiparallel β-strands and can undergo concentration dependant dimerization. We have tried to characterize folding intermediates of LBTI under equilibrium denaturation conditions. We have used various spectroscopic and microscopic techniques to understand the folding and misfolding pathways. LBTI forms molten globule structure at pH 2 and amyloidiogenic intermediate state (I(a)) at pH 4. pH induced Shifting of surface exposed hydrophobic patches and that followed by withdrawal of the lone tyrosine residue (Y69) towards nonpolar environment have been reported...
May 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28495970/high-throughput-biochemical-profiling-reveals-sequence-determinants-of-dcas9-off-target-binding-and-unbinding
#16
Evan A Boyle, Johan O L Andreasson, Lauren M Chircus, Samuel H Sternberg, Michelle J Wu, Chantal K Guegler, Jennifer A Doudna, William J Greenleaf
The bacterial adaptive immune system CRISPR-Cas9 has been appropriated as a versatile tool for editing genomes, controlling gene expression, and visualizing genetic loci. To analyze Cas9's ability to bind DNA rapidly and specifically, we generated multiple libraries of potential binding partners for measuring the kinetics of nuclease-dead Cas9 (dCas9) interactions. Using a massively parallel method to quantify protein-DNA interactions on a high-throughput sequencing flow cell, we comprehensively assess the effects of combinatorial mismatches between guide RNA (gRNA) and target nucleotides, both in the seed and in more distal nucleotides, plus disruption of the protospacer adjacent motif (PAM)...
May 23, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28484508/cohort-profile-the-complications-of-long-term-antiretroviral-therapy-study-in-uganda-coltart-a-prospective-clinical-cohort
#17
Billy Nsubuga Mayanja, Ivan Kasamba, Jonathan Levin, Ivan Namakoola, Patrick Kazooba, Jackson Were, Pontiano Kaleebu, Paula Munderi
BACKGROUND: Antiretroviral therapy (ART) improves the survival and quality of life of HIV-positive individuals, but the effects of long-term ART use do eventually manifest. The Complications of Long-Term Antiretroviral Therapy cohort study in Uganda (CoLTART) was established to investigate the metabolic and renal complications of long-term ART use among Ugandan adults. We describe the CoLTART study set-up, aims, objectives, study methods, and also report some preliminary cross-sectional study enrolment metabolic and renal complications data analysis results...
2017: AIDS Research and Therapy
https://www.readbyqxmd.com/read/28484292/correlation-of-oxidative-stress-markers-with-ultrasound-and-cardiotocography-parameters-with-hypertension-induced-pregnancy
#18
Dragica Draganovic, Nenad Lucic, Dragica Jojic, Snjezana Milicevic
INTRODUCTION: Pregnancy induced hypertension (PIH), especially preeclampsia, is a state of extremely increased oxidative stress (OS), due to decrease of antioxidant capacity in comparison to normotensive pregnant women. The consequences of increased state of oxidative stress with hypertension disorder in pregnancy are disorder of placental blood flow, intrauterine hypoxia of the fetus and disturbance in transfer of O2 manifested as pathological ultrasound (US) parameters (amount of amniotic fluid and biophysical profile of fetus) and cardiotocography parameters (CTG)...
March 2017: Acta Informatica Medica: AIM
https://www.readbyqxmd.com/read/28483196/bacterially-expressed-human-papillomavirus-type-6-and-11-bivalent-vaccine-characterization-antigenicity-and-immunogenicity
#19
Huirong Pan, Zhihai Li, Jin Wang, Shuo Song, Daning Wang, Minxi Wei, Ying Gu, Jun Zhang, Shaowei Li, Ningshao Xia
Human papillomavirus (HPV)-6 and HPV11 are the major etiological causes of condylomata acuminate. HPV neutralization by vaccine-elicited neutralizing antibodies can block viral infection and prevent subsequent disease. Currently, two commercially available HPV vaccines cover these two genotypes, expressed by Saccharomyces cerevisiae. Here we describe another HPV6/11 bivalent vaccine candidate derived from Escherichia coli. The soluble expression of N-terminally truncated L1 proteins was optimized to generate HPV6- and HPV11 L1-only virus-like particles (VLPs) as a scalable process...
May 31, 2017: Vaccine
https://www.readbyqxmd.com/read/28482223/biological-effects-of-%C3%AE-adrenergic-phentolamine-on-erythrocyte-hemeprotein-molecular-insights-from-biorecognition-behavior-protein-dynamics-and-flexibility
#20
Wei Peng, Fei Ding, Yu-Kui Peng, Yong Xie
Phentolamine is one of the most representative nonselective α-adrenoreceptor blocking agents, which have been proved to be owned various pharmacological actions. Unfortunately, whether erythrocytes in the veins intervene in biological behaviors of such drug are largely obscured. With the aid of multiple biophysical techniques, this scenario was to detailed explore the potential biorecognition between phentolamine and the hemeprotein in the cytosol of erythrocytes, and the influences of dynamic characters of protein during the bioreaction...
June 2017: Journal of Photochemistry and Photobiology. B, Biology
keyword
keyword
52090
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"