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Biophysical profile

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https://www.readbyqxmd.com/read/28211084/endothelial-cells-biophysical-biochemical-and-chromosomal-aberrancies-in-high-glucose-condition-within-the-diabetic-range
#1
Aysa Rezabakhsh, Elahe Nabat, Mina Yousefi, Soheila Montazersaheb, Omid Cheraghi, Amir Mehdizadeh, Farzaneh Fathi, Ali Akbar Movassaghpour, Nasrin Maleki-Dizaji, Reza Rahbarghazi, Alireza Garjani
To date, many studies have been conducted to find out the underlying mechanisms of hyperglycemia-induced complications in diabetes mellitus, attributed to the cellular pathologies of different cells-especially endothelial cells. However, there are still many ambiguities and unresolved issues to be clarified. Here, we investigated the alteration in biophysical and biochemical properties in human umbilical vein endothelial cells exposed to a high-glucose concentration (30mM), comparable to glucose content in type 2 diabetes mellitus, over a course of 120 hours...
February 17, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28195578/specific-membrane-capacitance-cytoplasm-conductivity-and-instantaneous-young-s-modulus-of-single-tumour-cells
#2
Ke Wang, Yang Zhao, Deyong Chen, Beiyuan Fan, Yulan Lu, Lianhong Chen, Rong Long, Junbo Wang, Jian Chen
As label-free biomarkers, biophysical properties of cells are widely used for cell type classification. However, intrinsic biophysical markers, e.g., specific membrane capacitance (Cspecific membrane), cytoplasm conductivity (σconductivity) and instantaneous Young's modulus (Einstantaneous) measured for hundreds of single cells were not yet reported. In this study, single cells in suspension (adherent cells treated with trypsin) were aspirated through a microfluidic constriction channel at 25 °C, and the entry processes and impedance profiles were recorded and translated to Cspecific membrane, σconductivity and Einstantaneous...
February 14, 2017: Scientific Data
https://www.readbyqxmd.com/read/28182292/metastatic-neuroblastoma-diagnosed-on-prenatal-sonographic-examination-performed-for-decreased-fetal-movement
#3
Hector O Chapa, Steven Gywnn Geddie, Rebecca Flores
We report a case of fetal neuroblastoma presenting with massive liver metastasis diagnosed during the biophysical profile sonographic examination performed for decreased fetal movement. The patient presented at 37 weeks' gestation with limited fetal movement over 24 hours. Biophysical profile showed marked polyhydramnios and an enlarged abdomen filled with a homogeneous mass lesion suspicious for liver metastasis. Primary urgent cesarean section was performed revealing a cachectic neonate with a rigid and grossly distended abdomen...
February 9, 2017: Journal of Clinical Ultrasound: JCU
https://www.readbyqxmd.com/read/28163113/the-estimation-of-the-traditionally-used-yarrow-achillea-millefolium-l-asteraceae-oil-extracts-with-anti-inflamatory-potential-in-topical-application
#4
Vanja Tadić, Ivana Arsić, Jelena Zvezdanović, Ana Zugić, Dragan Cvetković, Sava Pavkov
ETHNOPHARMACOLOGICAL RELEVANCE: Olive or sunflower oil yarrow extracts have been extensively used against inflammatory disorders and skin wound healing in traditional medicine. AIM OF THE STUDY: To evaluate oil yarrow extracts traditional use in treatment of topical/dermatological skin impairments, sodium lauryl sulfate test was applied, and in vivo measuring of the biophysical parameters (erythema index, skin capacitance and the pH of the skin) in the artificially irritated skin was performed...
February 2, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28163018/multiplexed-fc-array-for-evaluation-of-antigen-specific-antibody-effector-profiles
#5
Eric P Brown, Karen G Dowell, Austin W Boesch, Erica Normandin, Alison E Mahan, Thach Chu, Dan H Barouch, Chris Bailey-Kellogg, Galit Alter, Margaret E Ackerman
Antibodies are widely considered to be a frequent primary and often mechanistic correlate of protection of approved vaccines; thus evaluating the antibody response is of critical importance in attempting to understand and predict the efficacy of novel vaccine candidates. Historically, antibody responses have been analyzed by determining the titer of the humoral response using measurements such as an ELISA, neutralization, or agglutination assays. In the simplest case, sufficiently high titers of antibody against vaccine antigen(s) are sufficient to predict protection...
February 2, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28158217/3-4-dihydroxyphenylacetate-2-3-dioxygenase-from-pseudomonas-aeruginosa-an-fe-ii-containing-enzyme-with-fast-turnover
#6
Soraya Pornsuwan, Somchart Maenpuen, Philaiwarong Kamutira, Pratchaya Watthaisong, Kittisak Thotsaporn, Chanakan Tongsook, Maneerat Juttulapa, Sarayut Nijvipakul, Pimchai Chaiyen
3,4-dihydroxyphenylacetate (DHPA) dioxygenase (DHPAO) from Pseudomonas aeruginosa (PaDHPAO) was overexpressed in Escherichia coli and purified to homogeneity. As the enzyme lost activity over time, a protocol to reactivate and conserve PaDHPAO activity has been developed. Addition of Fe(II), DTT and ascorbic acid or ROS scavenging enzymes (catalase or superoxide dismutase) was required to preserve enzyme stability. Metal content and activity analyses indicated that PaDHPAO uses Fe(II) as a metal cofactor. NMR analysis of the reaction product indicated that PaDHPAO catalyzes the 2,3-extradiol ring-cleavage of DHPA to form 5-carboxymethyl-2-hydroxymuconate semialdehyde (CHMS) which has a molar absorptivity of 32...
2017: PloS One
https://www.readbyqxmd.com/read/28139759/the-intervening-domain-from-mecp2-enhances-the-dna-affinity-of-the-methyl-binding-domain-and-provides-an-independent-dna-interaction-site
#7
Rafael Claveria-Gimeno, Pilar M Lanuza, Ignacio Morales-Chueca, Olga C Jorge-Torres, Sonia Vega, Olga Abian, Manel Esteller, Adrian Velazquez-Campoy
Methyl-CpG binding protein 2 (MeCP2) preferentially interacts with methylated DNA and it is involved in epigenetic regulation and chromatin remodelling. Mutations in MeCP2 are linked to Rett syndrome, the leading cause of intellectual retardation in girls and causing mental, motor and growth impairment. Unstructured regions in MeCP2 provide the plasticity for establishing interactions with multiple binding partners. We present a biophysical characterization of the methyl binding domain (MBD) from MeCP2 reporting the contribution of flanking domains to its structural stability and dsDNA interaction...
January 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28119456/nectin-like-4-complexes-with-choline-transporter-like-protein-1-and-regulates-schwann-cell-choline-homeostasis-and-lipid-biogenesis-in-vitro
#8
Corey Heffernan, Mohit R Jain, Tong Liu, Hyosung Kim, Kevin Barretto, Hong Li, Patrice Maurel
Nectin-like 4 (Necl4, Cadm4) is a Schwann cell-specific cell adhesion molecule that promotes axo-glial interactions. In vitro and in vivo studies have shown that Necl4 is necessary for proper peripheral nerve myelination. However the molecular mechanisms that are regulated by Necl4 and affect peripheral myelination currently remain unclear. We used an in vitro approach to start identifying some of the mechanisms that could explain Necl4 function. Using mass spectrometry and Western blot techniques, we have identified Choline Transporter-Like 1 (CTL1) as a putative complexing partner with Necl4...
January 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28117825/mapping-the-binding-site-of-an-aptamer-on-atp-using-microscale-thermophoresis
#9
Clemens Entzian, Thomas Schubert
Characterization of molecular interactions in terms of basic binding parameters such as binding affinity, stoichiometry, and thermodynamics is an essential step in basic and applied science. MicroScale Thermophoresis (MST) is a sensitive biophysical method to obtain this important information. Relying on a physical effect called thermophoresis, which describes the movement of molecules through temperature gradients, this technology allows for the fast and precise determination of binding parameters in solution and allows the free choice of buffer conditions (from buffer to lysates/sera)...
January 7, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28109791/identification-of-a-selective-inhibitor-of-transforming-growth-factor-%C3%AE-activated-kinase-1-by-biosensor-based-screening-of-focused-libraries
#10
Takaaki Miura, Atsushi Matsuo, Terushige Muraoka, Mitsuaki Ide, Kenji Morikami, Takayuki Kamikawa, Masamichi Nishihara, Hirotaka Kashiwagi
Transforming growth factor-β activated kinase 1 (TAK1), a member of the mitogen-activated protein kinase kinase kinase family, plays an essential role in mediating signals from various pro-inflammatory cytokines and therefore may be a good target for developing anti-inflammation agents. Herein, we report our efforts to identify TAK1 inhibitors with a good selectivity profile with which to initiate medicinal chemistry. Instead of resorting to a high-throughput screening campaign, we performed biosensor-based biophysical screening for a limited number of compounds by taking advantage of existing knowledge on kinase inhibitors...
December 29, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28099727/association-of-biochemical-markers-with-the-severity-of-pre-eclampsia
#11
Ahmed M Maged, Gamal Aid, Nehal Bassiouny, Doaa S Eldin, Sherif Dahab, Nevein K Ghamry
OBJECTIVE: To assess the association between pre-eclampsia severity and biochemical and ultrasonography markers. METHODS: A retrospective study was undertaken of women with severe pre-eclampsia (group 1, n=90), mild pre-eclampsia (group 2, n=90), or a normal pregnancy (group 3, n=90) who attended a hospital in Egypt in October 2013-April 2015. Associations between pre-eclampsia and biochemical, cardiotocography, and ultrasonography markers were investigated. RESULTS: There were significant differences between the groups in C-reactive protein (331...
February 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28088651/limitations-in-biexponential-fitting-of-nmr-inversion-recovery-curves
#12
Mohammed Salman Shazeeb, Christopher H Sotak
NMR relaxation agents have long been employed as contrast agents in MRI. In many cases, the contrast agent is confined to either (i) the vascular and/or extracellular compartment (EC), as is the case with gadolinium(III)-based agents, or (ii) the intracellular compartment (IC), as is the case with manganese(II) ions. The compartmentalization of contrast agents often results in tissue-water (1)H relaxation profiles that are well modeled as biexponential. It has long been recognized that water exchange between compartments modifies the biexponential relaxation parameters (amplitudes and rate constants) from those that would be found in the absence of exchange...
January 6, 2017: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/28088457/preformulation-characterization-stabilization-and-formulation-design-for-the-acrylodan-labeled-glucose-binding-protein-sm4-ac
#13
Neha Sahni, Rajoshi Chaudhuri, John M Hickey, Prakash Manikwar, Ajit D'Souza, Andrew Metters, Sangeeta B Joshi, C Russell Middaugh, David B Volkin
This study describes the physicochemical characterization, stabilization, and formulation design of SM4-AC, an acrylodan-labeled glucose/galactose-binding protein for use in a continuous glucose monitoring device. The physical stability profile of SM4-AC as a function of pH and temperature was monitored using a combination of biophysical techniques and the resulting physical stability profile was visualized using an empirical phase diagram. Forced degradation chemical stability studies (Asn deamidation, Met oxidation) of SM4-AC were performed using a combination of capillary isoelectric focusing, peptide mapping, and reversed-phase HPLC...
January 11, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28076590/effect-of-maternal-exercises-on-biophysical-fetal-and-maternal-parameters-a-transversal-study
#14
Caroline Mombaque Dos Santos, Wendel Mombaque Dos Santos, Francisco Maximiliano Pancich Gallarreta, Camila Pigatto, Luiz Osório Cruz Portela, Edson Nunes de Morais
Objective: To evaluate the acute effects of maternal and fetal hemodynamic responses in pregnant women submitted to fetal Doppler and an aerobic physical exercise test according to the degree of effort during the activity and the impact on the well-being. Methods: Transversal study with low risk pregnant women, obtained by convenience sample with gestational age between 26 to 34 weeks. The participants carry out a progressive exercise test. Results: After the exercise session, reduced resistance (p=0...
October 2016: Einstein
https://www.readbyqxmd.com/read/28074994/-normoglycemic-diabetic-ketoacidosis-in-pregnancy-report-of-one-case
#15
Margarita Rivas M, Pamela Belmar Z, Pilar Durruty A, Lilian Sanhueza M, Gloria López S
Normoglycemic diabetic ketoacidosis should be suspected in pregnant women presenting nausea, vomiting, abdominal pain and anorexia. We report a 39 years old woman with a 32 weeks pregnancy who sought emergency care due to hyperemesis. She was hospitalized with the following diagnoses: pregnancy hypertension syndrome, gestational diabetes, morbid obesity and poor prenatal control. The evaluation of the feto-placental unit showed perception of fetal movements, non-reactive non-stress baseline record and a biophysical profile of 6/8...
October 2016: Revista Médica de Chile
https://www.readbyqxmd.com/read/28074920/a-comprehensive-evaluation-of-the-activity-and-selectivity-profile-of-ligands-for-rgd-binding-integrins
#16
Tobias G Kapp, Florian Rechenmacher, Stefanie Neubauer, Oleg V Maltsev, Elisabetta A Cavalcanti-Adam, Revital Zarka, Ute Reuning, Johannes Notni, Hans-Jürgen Wester, Carlos Mas-Moruno, Joachim Spatz, Benjamin Geiger, Horst Kessler
Integrins, a diverse class of heterodimeric cell surface receptors, are key regulators of cell structure and behaviour, affecting cell morphology, proliferation, survival and differentiation. Consequently, mutations in specific integrins, or their deregulated expression, are associated with a variety of diseases. In the last decades, many integrin-specific ligands have been developed and used for modulation of integrin function in medical as well as biophysical studies. The IC50-values reported for these ligands strongly vary and are measured using different cell-based and cell-free systems...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28071973/collagen-cross-linking-biophysical-biochemical-and-biological-response-analysis
#17
Luis M Delgado, Kieran Fuller, Dimitrios I Zeugolis
Extracted forms of collagen are subjected to chemical cross-linking to enhance their stability. However, traditional cross-linking approaches are associated with toxicity and inflammation. This work investigates the stabilization capacity, cytotoxicity and inflammatory response of collagen scaffolds cross-linked with glutaraldehyde (GTA), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, 4-arm polyethylene glycol (PEG) succinimidyl glutarate (4SP), genipin (GEN), and oleuropein. Although all cross-linking methods reduced free amine groups, variable data were obtained with respect to denaturation temperature, resistance to collagenase digestion, and mechanical properties...
February 6, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28065847/a-drosophila-model-of-gdap1-function-reveals-the-involvement-of-insulin-signalling-in-the-mitochondria-dependent-neuromuscular-degeneration
#18
Víctor López Del Amo, Martina Palomino-Schätzlein, Marta Seco-Cervera, José Luis García-Giménez, Federico Vicente Pallardó, Antonio Pineda-Lucena, Máximo Ibo Galindo
Charcot-Marie-Tooth disease is a rare peripheral neuropathy for which there is no specific treatment. Some forms of Charcot-Marie-Tooth are due to mutations in the GDAP1 gene. A striking feature of mutations in GDAP1 is that they have a variable clinical manifestation, according to disease onset and progression, histology and mode of inheritance. Studies in cellular and animal models have revealed a role of GDAP1 in mitochondrial morphology and distribution, calcium homeostasis and oxidative stress. To get a better understanding of the disease mechanism we have generated models of over-expression and RNA interference of the Drosophila Gdap1 gene...
January 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28062644/ligand-induced-conformational-changes-in-prolyl-oligopeptidase-a-kinetic-approach
#19
R Van Elzen, E Schoenmakers, I Brandt, P Van Der Veken, A M Lambeir
Most kinetic studies of prolyl oligopeptidase (PREP) were performed with the porcine enzyme using modified peptide substrates. Yet recent biophysical studies used the human homolog. Therefore, the aim of this study was to compare the kinetic behavior of human and porcine PREP, as well as to find a suitable method to study enzyme kinetics with an unmodified biological substrate. It was found that human PREP behaves identically to the porcine homolog, displaying a double bell-shaped pH profile and a pH-dependent solvent kinetic isotope effect of the kcat/Km, features that set it apart from the related exopeptidase dipeptidyl peptidase IV (DPP IV)...
January 5, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/28057542/an-fc-silenced-igg1-format-with-extended-half-life-designed-for-improved-stability
#20
M Jack Borrok, Neil Mody, Xiaojun Lu, Megan L Kuhn, Herren Wu, William F Dall'Acqua, Ping Tsui
Multiple mutation combinations in the IgG Fc have been characterized to tailor immune effector function or IgG serum persistence to fit desired biological outcomes for monoclonal antibody therapeutics. An unintended consequence of introducing mutations in the Fc (particularly the CH2 domain) can be a reduction in biophysical stability which can correlate with increased aggregation propensity, poor manufacturability, and lower solubility. Herein, we characterize the changes in IgG conformational and colloidal stability when 2 sets of CH2 mutations "TM" (L234F/L235E/P331S) and "YTE" (M252Y/S254T/T256E) are combined to generate an antibody format lacking immune receptor binding and exhibiting extended half-life...
January 3, 2017: Journal of Pharmaceutical Sciences
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