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Saccharomyces cerevisiae

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https://www.readbyqxmd.com/read/29677095/milling-the-mistletoe-nanotechnological-conversion-of-african-mistletoe-loranthus-micranthus-intoantimicrobial-materials
#1
Muhammad Sarfraz, Sharoon Griffin, Tamara Gabour Sad, Rama Alhasan, Muhammad Jawad Nasim, Muhammad Irfan Masood, Karl Herbert Schäfer, Chukwunonso E C C Ejike, Cornelia M Keck, Claus Jacob, Azubuike P Ebokaiwe
Nanosizing represents a straight forward technique to unlock the biological activity of complex plant materials. The aim of this study was to develop herbal nanoparticles with medicinal value from dried leaves and stems of Loranthus micranthus with the aid of ball-milling, high speed stirring, and high-pressure homogenization techniques. The milled nanoparticles were characterized using laser diffraction analysis, photon correlation spectroscopy analysis, and light microscopy. The average size of leaf nanoparticles was around 245 nm and that of stem nanoparticles was around 180 nm...
April 20, 2018: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29675837/batch-bioethanol-production-via-the-biological-and-chemical-saccharification-of-some-egyptian-marine-macroalgae
#2
Ramadan M Soliman, Sherif A Younis, Nour Sh El-Gendy, Soha S M Mostafa, Seham A El-Temtamy, Ahmed I Hashim
AIMS: Marine seaweeds (macroalgae) cause eutrophication problem and affects the touristic activities. The success of the production of the third generation bioethanol from marine macroalgae depends mainly on the development of an ecofriendly and eco-feasible pretreatment (i.e. hydrolysis) technique, a highly effective saccharification step and finally an efficient bioethanol fermentation step. Therefore, this study aimed to investigate the potentiality of different marine macroalgal strains, collected from Egyptian coasts, for bioethanol production via different saccharification processes...
April 19, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29675568/effect-of-selenium-on-lipid-and-amino-acid-metabolism-in-yeast-cells
#3
Marek Kieliszek, Stanisław Błażejak, Anna Bzducha-Wróbel, Anna M Kot
This article discusses the effect of selenium in aqueous solutions on aspects of lipid and amino acid metabolism in the cell biomass of Saccharomyces cerevisiae MYA-2200 and Candida utilis ATCC 9950 yeasts. The yeast biomass was obtained by using waste products (potato wastewater and glycerol). Selenium, at a dose of 20 mg/L of aqueous solution, affected the differentiation of cellular morphology. Yeast enriched with selenium was characterized by a large functional diversity in terms of protein and amino acid content...
April 19, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29675464/heritable-stress-response-dynamics-revealed-by-single-cell-genealogy
#4
Meenakshi Chatterjee, Murat Acar
Cells often respond to environmental stimuli by activating specific transcription factors. Upon exposure to glucose limitation stress, it is known that yeast Saccharomyces cerevisiae cells dephosphorylate the general stress response factor Msn2, leading to its nuclear localization, which in turn activates the expression of many genes. However, the precise dynamics of Msn2 nucleocytoplasmic translocations and whether they are inherited over multiple generations in a stress-dependent manner are not well understood...
April 2018: Science Advances
https://www.readbyqxmd.com/read/29674999/use-of-torulaspora-delbrueckii-co-fermentation-with-two-saccharomyces-cerevisiae-strains-with-different-aromatic-characteristic-to-improve-the-diversity-of-red-wine-aroma-profile
#5
Bo-Qin Zhang, Yu Luan, Chang-Qing Duan, Guo-Liang Yan
The use of selected Saccharomyces and non- Saccharomyces strains as mixed starters has advantages over pure fermentation due to achieving wine products with distinctive and diversified aroma expected by consumers. To obtain a way to improve the aroma diversity and increase the differentiation of wine product, in this study, the aromatic effect of multi-culture of indigenous Torulaspora delbrueckii ( TD 12), simultaneous and sequential inoculation with two Saccharomyces strains (indigenous icewine yeast SC 45 and commercial yeast BDX) with different enological characteristics were investigated in laboratory-scale 20 L fermenter, respectively...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29673976/effect-of-ultrasonic-field-on-the-enzyme-activities-and-ion-balance-of-potential-pathogen-saccharomyces-cerevisiae
#6
Junyan Liu, Lin Li, Lizhen Zhou, Bing Li, Zhenbo Xu
OBJECTIVES: This study aimed at investigating the enzyme activities and ion concentrations in potential pathogen S.cerevisiae upon ultrasonic treatment. METHODS: The activities of ATPase and antioxidase were identified by ATPase, SOD, and CAT assay kits following the instructions. Extracellular Ca2+ and K+ concentrations were determined in an atomic absorption spectrometer with calcium and potassium hollow-cathode lamps as radiation sources. RESULTS: SOD and CAT activities were enhanced by relatively low ultrasonic power at early time points and reduced to lower levels...
April 16, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29673353/modulation-of-ovine-sbd-1-expression-by-saccharomyces-cerevisiae-in-ovine-ruminal-epithelial-cells
#7
Xin Jin, Man Zhang, Xue-Min Zhu, Yan-Ru Fan, Chen-Guang Du, Hua-Er Bao, Siri-Guleng Xu, Qiao-Zhen Tian, Yun-He Wang, Yin-Feng Yang
BACKGROUND: The ovine rumen is involved in host defense responses and acts as the immune interface with the environment. The ruminal mucosal epithelium plays an important role in innate immunity and secretes antimicrobial innate immune molecules that have bactericidal activity against a variety of pathogens. Defensins are cationic peptides that are produced by the mucosal epithelia and have broad-spectrum antimicrobial activity. Sheep β-defensin-1 (SBD-1) is one of the most important antibacterial peptides in the rumen...
April 19, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29672693/metabolic-and-genomic-characterization-of-stress-tolerant-industrial-saccharomyces-cerevisiae-strains-from-talens-assisted-multiplex-editing
#8
Yuman Gan, Yuping Lin, Yufeng Guo, Xianni Qi, Qinhong Wang
TALENs-assisted multiplex editing (TAME) toolbox was previously established and used to successfully enhance ethanol-stress tolerance of S. cerevisiae laboratory strain. Here, the TAME toolbox was harnessed to improve and elucidate stress tolerances of S. cerevisiae industrial strain. One osmotolerant strain and one thermotolerant strain were selected from the mutant library generated by TAME at corresponding stress conditions, and exhibited 1.2- to 1.3-fold increases of fermentation capacities, respectively...
April 17, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29672528/saccharomyces-cerevisiae-as-a-platform-for-assessing-sphingolipid-lipid-kinase-inhibitors
#9
Yugesh Kharel, Sayeh Agah, Tao Huang, Anna J Mendelson, Oluwafunmilayo T Eletu, Peter Barkey-Bircann, James Gesualdi, Jeffrey S Smith, Webster L Santos, Kevin R Lynch
Successful medicinal chemistry campaigns to discover and optimize sphingosine kinase inhibitors require a robust assay for screening chemical libraries and for determining rank order potencies. Existing assays for these enzymes are laborious, expensive and/or low throughput. The toxicity of excessive levels of phosphorylated sphingoid bases for the budding yeast, Saccharomyces cerevisiae, affords an assay wherein inhibitors added to the culture media rescue growth in a dose-dependent fashion. Herein, we describe our adaptation of a simple, inexpensive, and high throughput assay for assessing inhibitors of sphingosine kinase types 1 and 2 as well as ceramide kinase and for testing enzymatic activity of sphingosine kinase type 2 mutants...
2018: PloS One
https://www.readbyqxmd.com/read/29671328/metabolic-engineering-of-saccharomyces-cerevisiae-for-high-level-production-of-salidroside-from-glucose
#10
Jingjie Jiang, Hua Yin, Shuai Wang, Yibin Zhuang, Shaowei Liu, Tao Liu, Yanhe Ma
Salidroside is an important plant-derived aromatic compound with diverse biological properties. Due to inadequate natural resources, the supply of salidroside is currently limited. In this work, we engineered the production of salidroside in yeast. First, the aromatic aldehyde synthase (AAS) from Petroselinum crispum was overexpressed in Saccharomyces cerevisiae when combined with endogenous Ehrlich pathway to produce tyrosol from tyrosine. Glucosyltransferases from different resources were tested for ideal production of salidroside in the yeast...
April 19, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29670542/atp-synthase-diseases-of-mitochondrial-genetic-origin
#11
REVIEW
Alain Dautant, Thomas Meier, Alexander Hahn, Déborah Tribouillard-Tanvier, Jean-Paul di Rago, Roza Kucharczyk
Devastating human neuromuscular disorders have been associated to defects in the ATP synthase. This enzyme is found in the inner mitochondrial membrane and catalyzes the last step in oxidative phosphorylation, which provides aerobic eukaryotes with ATP. With the advent of structures of complete ATP synthases, and the availability of genetically approachable systems such as the yeast Saccharomyces cerevisiae , we can begin to understand these molecular machines and their associated defects at the molecular level...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29670105/viral-proteins-as-a-potential-driver-of-histone-depletion-in-dinoflagellates
#12
Nicholas A T Irwin, Benjamin J E Martin, Barry P Young, Martin J G Browne, Andrew Flaus, Christopher J R Loewen, Patrick J Keeling, LeAnn J Howe
Within canonical eukaryotic nuclei, DNA is packaged with highly conserved histone proteins into nucleosomes, which facilitate DNA condensation and contribute to genomic regulation. Yet the dinoflagellates, a group of unicellular algae, are a striking exception to this otherwise universal feature as they have largely abandoned histones and acquired apparently viral-derived substitutes termed DVNPs (dinoflagellate-viral-nucleoproteins). Despite the magnitude of this transition, its evolutionary drivers remain unknown...
April 18, 2018: Nature Communications
https://www.readbyqxmd.com/read/29668941/robust-pleiotropic-drug-resistance-5-pdr5-mediated-multidrug-resistance-is-vigorously-maintained-in-saccharomyces-cerevisiae-cells-during-glucose-and-nitrogen-limitation
#13
Hadiar Rahman, Joshua Carneglia, Molly Lausten, Michael Robertello, John Choy, John Golin
Saccharomyces cerevisiae has sophisticated nutrient-sensing programs for responding to harsh environments containing limited nutrients. As a result, yeast cells can live in diverse environments, including animals, as a commensal or a pathogen. Because they live in mixed populations with other organisms that excrete toxic chemicals, it is of interest to know whether yeast cells maintain functional multidrug resistance mechanisms during nutrient stress. We measured the activity of Pdr5, the major Saccharomyces drug efflux pump under conditions of limiting nutrients...
April 16, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29668115/yeast-identification-during-fermentation-of-turkish-gemlik-olives
#14
Gamze Mujdeci, María Arévalo-Villena, Z Yesim Ozbas, Ana Briones Pérez
Naturally fermented black table olives of the Gemlik variety are one of the most consumed fermented products in Turkey. The objective of this work was to identify yeast strains isolated during their natural fermentation by using Restriction Fragments Lengths Polymorphism-Polimerase Chain Reaction (RFLP-PCR) and DNA sequencing methods. The study also focused on determining the effect of regional differences on yeast microflora of naturally fermented Gemlik olives. A total of 47 yeast strains belonging to 12 different species which had been previously isolated from the natural brine of Akhisar and Iznik-Gemlik cv...
April 18, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29668035/metaproteomics-of-microbiota-in-naturally-fermented-soybean-paste-da-jiang
#15
Ping Zhang, Pengfei Zhang, Mengxi Xie, Feiyu An, Boshu Qiu, Rina Wu
Da-jiang is a typical traditional fermented soybean product in China. At present, the proteins in da-jiang are needed to be explored. The composition and species of microbial proteins in traditional fermented da-jiang were analyzed by metaproteomics based on sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The results showed that the number and variety of microbial proteins in the traditional fermented da-jiang from different regions were different...
April 18, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29666939/a-transcriptomic-analysis-of-saccharomyces-cerevisiae-under-the-stress-of-2-phenylethanol
#16
Danfeng Jin, Bintao Gu, Dawei Xiong, Guochang Huang, Xiaoping Huang, Lan Liu, Jun Xiao
2-Phenylethanol (2-PE) is a kind of advanced aromatic alcohol with rose fragrance, which is wildly used for the deployment of flavors and fragrances. Microbial transformation is the most feasible method for the production of natural 2-PE. But a bottleneck problem is the toxicity of 2-PE on the cells. The molecular mechanisms of the toxic effect of 2-PE to Saccharomyces cerevisiae are not well studied. In this study, we analyzed the transcriptomes of S. cerevisiae in the media with and without 2-PE, respectively, using Illumina RNA-Seq technology...
April 17, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29666537/antioxidant-and-antidiabetic-activities-of-vanadium-binding-proteins-purified-from-the-sea-squirt-halocynthia-roretzi
#17
Minoli Anuththara Gunasinghe, Sang Moo Kim
Sea squirts accumulate vanadium compounds with potent antidiabetic activity, which are involved in immune defense. In this study, vanadium concentrations of fresh blood plasma, intestine, and muscle of the sea squirt Halocynthia roretzi were 6.3, 3.7 and 2.1 mg/kg respectively. Two vanadium binding proteins (VBPs) from blood plasma and intestine were purified through (NH4 )2 SO4 precipitation, and DEAE-Sepharose ion exchange and Sephacryl S-200 HR gel filtration chromatography, in that order. The purity and yield of the intestine and blood plasma vanadium binding proteins, VBPintestine and VBPblood plasma, were 13...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29665954/candida-species-in-tchapalo-and-bangui-two-traditional-alcoholic-beverages-from-c%C3%A3-te-d-ivoire
#18
Laurence A N Egue, Florent K N'guessan, Solange Aka-Gbezo, Jean-Paul K M Bouatenin, Marina Koussemon-Camara
The increase of infections due to non-Candida albicans species made it very necessary to conduct adequate characterization to be able to identify the species of Candida isolated from traditional fermented foods. In this study, based on their hue on Candida Chromogenic Agar medium, a total of 136 yeast strains were isolated from tchapalo and bangui. Molecular identification based on PCR-RFLP of internal transcribed spacers of rDNA (ITS) and sequencing of the ITS and the D1/D2 regions allowed us to assign these isolates to seven species: Candida tropicalis, Candida inconspicua, Candida rugosa, Saccharomyces cerevisiae, Kluyveromyces marxianus, Hanseniaspora guilliermondii, Trichosporon asahii...
May 2018: Fungal Biology
https://www.readbyqxmd.com/read/29665400/tolerant-industrial-yeast-saccharomyces-cerevisiae-posses-a-more-robust-cell-wall-integrity-signaling-pathway-against-2-furaldehyde-and-5-hydroxymethyl-2-furaldehyde
#19
Z Lewis Liu, Xu Wang, Scott A Weber
Cell wall integrity signaling pathway in Saccharomyces cerevisiae is a conserved function for detecting and responding to cell stress conditions but less understood for industrial yeast. We examined gene expression dynamics for a tolerant industrial yeast strain NRRL Y-50049 in response to challenges of furfural and HMF through comparative quantitative gene expression analysis using pathway-based qRT-PCR array assays. All tested genes from Y-50049, except for MLP2, demonstrated more resistant and significantly increased gene expression than that from a laboratory strain BY4741...
April 14, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29665361/rna-seq-analysis-of-global-transcriptomic-changes-suggests-a-role-for-the-mapk-pathway-and-carbon-metabolism-in-cell-wall-maintenance-in-a-saccharomyces-cerevisiae-fks1-mutant
#20
Cong Huang, Fengguang Zhao, Ying Lin, Suiping Zheng, Shuli Liang, Shuangyan Han
FKS1 encodes a β-1,3-glucan synthase, which is a key player in cell wall assembly in Saccharomyces cerevisiae. Here we analyzed the global transcriptomic changes in the FKS1 mutant to establish a correlation between the changes in the cell wall of the FKS1 mutant and the molecular mechanism of cell wall maintenance. These transcriptomic profiles showed that there are 1151 differentially expressed genes (DEGs) in the FKS1 mutant. Through KEGG pathway analysis of the DEGs, the MAPK pathway and seven pathways involved in carbon metabolism were significantly enriched...
April 14, 2018: Biochemical and Biophysical Research Communications
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