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Saccharomyces cerevisiae

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https://www.readbyqxmd.com/read/28225760/reconstitution-of-the-tubular-endoplasmic-reticulum-network-with-purified-components
#1
Robert E Powers, Songyu Wang, Tina Y Liu, Tom A Rapoport
Organelles display characteristic morphologies that are intimately tied to their cellular function, but how organelles are shaped is poorly understood. The endoplasmic reticulum is particularly intriguing, as it comprises morphologically distinct domains, including a dynamic network of interconnected membrane tubules. Several membrane proteins have been implicated in network formation, but how exactly they mediate network formation and whether they are all required are unclear. Here we reconstitute a dynamic tubular membrane network with purified endoplasmic reticulum proteins...
February 22, 2017: Nature
https://www.readbyqxmd.com/read/28225104/coplanar-electrode-microfluidic-chip-enabling-accurate-sheathless-impedance-cytometry
#2
Adele De Ninno, Vito Errico, Francesca Romana Bertani, Luca Businaro, Paolo Bisegna, Federica Caselli
Microfluidic impedance cytometry offers a simple non-invasive method for single-cell analysis. Coplanar electrode chips are especially attractive due to ease of fabrication, yielding miniaturized, reproducible, and ultimately low-cost devices. However, their accuracy is challenged by the dependence of the measured signal on particle trajectory within the interrogation volume, that manifests itself as an error in the estimated particle size, unless any kind of focusing system is used. In this paper, we present an original five-electrode coplanar chip enabling accurate particle sizing without the need for focusing...
February 22, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28224439/complementary-function-of-two-transketolase-isoforms-from-moniliella-megachiliensis-in-relation-to-stress-response
#3
Hisashi Iwata, Yosuke Kobayashi, Daiki Mizushima, Taisuke Watanabe, Jun Ogihara, Takafumi Kasumi
Two transketolase isogenes, MmTKL1 and MmTKL2, isolated from Moniliella megachiliensis were investigated for their roles in stress response and erythritol biosynthesis. The encoded proteins were highly homologous in amino acid sequence and domain structure. Two stress response elements (STREs) were found upstream of MmTKL1, while no STRE was found upstream of MmTKL2. In contrast, two Ap-1 elements were present upstream of MmTKL2, but none were detected upstream of MmTKL1. MmTKL2 partially complemented the aromatic amino acid auxotrophy of a Saccharomyces cerevisiae tkl1 deletion mutant, suggesting that at least one of the MmTKLs functioned as a transketolase in vivo...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28223968/effect-of-temperature-on-the-prevalence-of-saccharomyces-non-cerevisiae-species-against-a-s-cerevisiae-wine-strain-in-wine-fermentation-competition-physiological-fitness-and-influence-in-final-wine-composition
#4
Javier Alonso-Del-Real, María Lairón-Peris, Eladio Barrio, Amparo Querol
Saccharomyces cerevisiae is the main microorganism responsible for the fermentation of wine. Nevertheless, in the last years wineries are facing new challenges due to current market demands and climate change effects on the wine quality. New yeast starters formed by non-conventional Saccharomyces species (such as S. uvarum or S. kudriavzevii) or their hybrids (S. cerevisiae x S. uvarum and S. cerevisiae x S. kudriavzevii) can contribute to solve some of these challenges. They exhibit good fermentative capabilities at low temperatures, producing wines with lower alcohol and higher glycerol amounts...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28223526/hypermutation-signature-reveals-a-slippage-and-realignment-model-of-translesion-synthesis-by-rev3-polymerase-in-cisplatin-treated-yeast
#5
Romulo Segovia, Yaoqing Shen, Scott A Lujan, Steven J M Jones, Peter C Stirling
Gene-gene or gene-drug interactions are typically quantified using fitness as a readout because the data are continuous and easily measured in high throughput. However, to what extent fitness captures the range of other phenotypes that show synergistic effects is usually unknown. Using Saccharomyces cerevisiae and focusing on a matrix of DNA repair mutants and genotoxic drugs, we quantify 76 gene-drug interactions based on both mutation rate and fitness and find that these parameters are not connected. Independent of fitness defects, we identified six cases of synthetic hypermutation, where the combined effect of the drug and mutant on mutation rate was greater than predicted...
February 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28223409/asc1-hel2-and-slh1-couple-translation-arrest-to-nascent-chain-degradation
#6
Cole Stone Sitron, Joseph Hun Park, Onn Brandman
Premature arrest of protein synthesis within the open reading frame elicits a protective response that degrades the incomplete nascent chain. In this response, arrested 80S ribosomes are split into their large and small subunits, allowing assembly of the Ribosome Quality control Complex (RQC), which targets nascent chains for degradation. How the cell recognizes arrested nascent chains among the vast pool of actively translating polypeptides is poorly understood. We systematically examined translation arrest and modification of nascent chains in Saccharomyces cerevisiae to characterize the steps that couple arrest to RQC targeting...
February 21, 2017: RNA
https://www.readbyqxmd.com/read/28223030/heterologous-expression-of-a-rice-metallothionein-isoform-osmti-1b-in-saccharomyces-cerevisiae-enhances-cadmium-hydrogen-peroxide-and-ethanol-tolerance
#7
Zahra Ansarypour, Azar Shahpiri
Metallothioneins are a superfamily of low-molecular-weight, cysteine (Cys)-rich proteins that are believed to play important roles in protection against metal toxicity and oxidative stress. The main purpose of this study was to investigate the effect of heterologous expression of a rice metallothionein isoform (OsMTI-1b) on the tolerance of Saccharomyces cerevisiae to Cd(2+), H2O2 and ethanol stress. The gene encoding OsMTI-1b was cloned into p426GPD as a yeast expression vector. The new construct was transformed to competent cells of S...
February 4, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28221980/inactivation-of-yeast-on-grapes-by-plasma-activated-water-and-its-effects-on-quality-attributes
#8
Jian Guo, Kang Huang, Xiao Wang, Chenang Lyu, Nannan Yang, Yanbin Li, Jianping Wang
Plasma-activated water (PAW) is a promising nonthermal technology in food preservation and food safety. The inactivation efficacy of PAW was investigated against Saccharomyces cerevisiae CICC 1374 inoculated on grape berries. PAW30 and PAW60 were obtained by activating water with plasma for 30 and 60 min, respectively. Grapes were directly treated with PAW, and a 0.38- to 0.53-log CFU/ml reduction of S. cerevisiae was achieved in a time-dependent manner (P < 0.05). The oxidation-reduction potential and pH values of PAW30 and PAW60 were also in a time-dependent manner (P < 0...
February 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221940/development-of-multifunctional-active-film-and-its-application-in-modified-atmosphere-packaging-of-shiitake-mushrooms
#9
Hong Jiang Wang, Duck Soon An, Dong Sun Lee
Agar-based films with multiple functions (CO2 absorption, water vapor absorption, and antimicrobial activity) were developed, tested for their properties, and then applied to the packaging of fresh shiitake mushrooms as an insert label. The films were cast from an agar-based aqueous solution containing a dissolving plasticizer (glycerol), a CO2 absorbent (sodium carbonate [SC] alone or a combination of SC and sodium glycinate [SC-SG]), and a volatile antimicrobial agent (carvacrol [CRV]). The agar of the film matrix is designed to serve as a water vapor absorbent...
September 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221769/manipulation-of-the-precursor-supply-in-yeast-significantly-enhances-the-accumulation-of-prenylated-%C3%AE-carbolines
#10
Katja Backhaus, Lena Ludwig-Radtke, Xiulan Xie, Shu-Ming Li
The tryptophan derivative 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (MTCA) is present in many plants and foods including fermentation products of the baker's yeast Saccharomyces cerevisiae. MTCA is formed from tryp-tophan and acetaldehyde via a Pictet-Spengler reaction. In this study, up to 9 mg/l of MTCA were detected as a mixture of (1S,3S) and (1R,3S) isomers in a ratio of 2.2:1 in Saccharomyces cerevisiae cultures. To the best of our knowledge, this is the first report on the presence of MTCA in laboratory baker's yeast cultures...
February 21, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28221768/formation-of-nitrogenase-nifdk-tetramers-in-the-mitochondria-of-saccharomyces-cerevisiae
#11
Stefan Burén, Eric M Young, Elizabeth A Sweeny, Gema López-Torrejón, Marcel Veldhuizen, Christopher A Voigt, Luis M Rubio
Transferring the prokaryotic enzyme nitrogenase into a eukaryotic host with the final aim of developing N2 fixing cereal crops would revolutionize agricultural systems worldwide. Targeting it to mitochondria has potential advantages because of the organelle's high O2 consumption and the presence of bacterial-type iron-sulfur cluster biosynthetic machinery. In this study, we constructed 96 strains of Saccharomyces cerevisiae where transcriptional units comprising nine Azotobacter vinelandii nif genes (nifHDKUSMBEN) were integrated into the genome...
February 21, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28221018/low-efficiency-upconversion-nanoparticles-for-high-resolution-coalignment-of-near-infrared-and-visible-light-paths-on-a-light-microscope
#12
Sriramkumar Sundaramoorthy, Adrian Garcia Badaracco, Sophia M Hirsch, Jun Hong Park, Tim Davies, Julien Dumont, Mimi Shirasu-Hiza, Andrew C Kummel, Julie C Canman
The combination of near-infrared (NIR) and visible wavelengths in light microscopy for biological studies is increasingly common. For example, many fields of biology are developing the use of NIR for optogenetics, in which an NIR laser induces a change in gene expression and/or protein function. One major technical barrier in working with both NIR and visible light on an optical microscope is obtaining their precise coalignment at the imaging plane position. Photon upconverting particles (UCPs) can bridge this gap as they are excited by NIR light but emit in the visible range via an anti-Stokes luminescence mechanism...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28219720/lipidomics-in-research-on-yeast-membrane-lipid-homeostasis
#13
Anton I P M de Kroon
Mass spectrometry is increasingly used in research on membrane lipid homeostasis, both in analyses of the steady state lipidome at the level of molecular lipid species, and in pulse-chase approaches employing stable isotope-labeled lipid precursors addressing the dynamics of lipid metabolism. Here my experience with, and view on mass spectrometry-based lipid analysis is presented, with emphasis on aspects of quantification of membrane lipid composition of the yeast Saccharomyces cerevisiae.
February 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28219524/the-function-and-transcriptome-analysis-of-a-bzip-transcription-factor-cgap1-in-colletotrichum-gloeosporioides
#14
Xiaoyu Li, Yateng Wu, Zhiqiang Liu, Chenghui Zhang
Colletotrichum gloeosporioides is an important pathogen of anthracnose, which is able to infect numerous crops in tropical and subtropical regions, causing great economic losses. To investigate the fungal response to host-generated reactive oxygen species (ROS), we cloned and characterized the CgAP1 gene of C. gloeosporioides. CgAP1 encoded a bZIP transcription factor which had a bZIP DNA-binding domain and two cysteine-rich domains structurally and functionally related to Saccharomyces cerevisiae YAP1. Deletion of CgAP1 in C...
April 2017: Microbiological Research
https://www.readbyqxmd.com/read/28217994/structure-of-a-stable-g-hairpin
#15
Martin Gajarsky, Martina Lenarcic Zivkovic, Petr Stadlbauer, Bruno Pagano, Radovan Fiala, Jussara Amato, Lubomir Tomaska, Jiri Sponer, Janez Plavec, Lukas Trantirek
In this study, we report the first atomic resolution structure of a stable G-hairpin formed by a natively occurring DNA sequence. An 11-nt-long G-rich DNA oligonucleotide, 5'-d(GTGTGGGTGTG)-3', corresponding to the most abundant sequence motif in irregular telomeric DNA from Saccharomyces cerevisiae (yeast), is demonstrated to adopt a novel type of mixed parallel/antiparallel fold-back DNA structure, which is stabilized by dynamic G:G base pairs that transit between N1-carbonyl symmetric and N1-carbonyl, N7-amino base-pairing arrangements...
February 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28216107/an-endoplasmic-reticulum-engineered-yeast-platform-for-overproduction-of-triterpenoids
#16
Philipp Arendt, Karel Miettinen, Jacob Pollier, Riet De Rycke, Nico Callewaert, Alain Goossens
Saponins are a structurally diverse family of triterpenes that are widely found as main constituents in many traditional plant-based medicines and often have bioactivities of industrial interest. The heterologous production of triterpene saponins in microbes remains challenging and only limited successful pathway engineering endeavors have been reported. To improve the production capacities of a Saccharomyces cerevisiae saponin production platform, we assessed the effects of several hitherto unexplored gene knockout targets on the heterologous production of triterpenoids...
February 16, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28216106/metabolic-engineering-of-a-haploid-strain-derived-from-a-triploid-industrial-yeast-for-producing-cellulosic-ethanol
#17
Soo Rin Kim, Jeffrey M Skerker, In Iok Kong, Heejin Kim, Matthew J Maurer, Guo-Chang Zhang, Dairong Peng, Na Wei, Adam P Arkin, Yong-Su Jin
Many desired phenotypes for producing cellulosic biofuels are often observed in industrial Saccharomyces cerevisiae strains. However, many industrial yeast strains are polyploid and have low spore viability, making it difficult to use these strains for metabolic engineering applications. We selected the polyploid industrial strain S. cerevisiae ATCC 4124 exhibiting rapid glucose fermentation capability, high ethanol productivity, strong heat and inhibitor tolerance in order to construct an optimal yeast strain for producing cellulosic ethanol...
February 16, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28216105/-increasing-the-carbohydrate-storage-capacity-of-plants-by-engineering-a-glycogen-like-polymer-pool-in-the-cytosol
#18
Simona Eicke, David Seung, Barbara Egli, Emanuel A Devers, Sebastian Streb
Global demand for higher crop yields and for more efficient utilization of agricultural products will grow over the next decades. Here, we present a new concept for boosting the carbohydrate content of plants, by channeling photosynthetically fixed carbon into a newly engineered glucose polymer pool. We transiently expressed the starch/glycogen synthases from either Saccharomyces cerevisiae or Cyanidioschyzon merolae, together with the starch branching enzyme from C. merolae, in the cytosol of Nicotiana benthamiana leaves...
February 16, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28215338/investigating-symmetry-breaking-in-yeast-from-seeing-to-understanding
#19
P Singh, A Das, R Li
Diverse cellular functions require the establishment of cell polarity. Although the morphological manifestation of cell polarity can be complex, common principles and conserved regulatory pathways can be studied leveraging the powerful genetic tools in the budding yeast Saccharomyces cerevisiae. A vast array of genetic and cell biological tools and interdisciplinary approaches have been employed to understand the establishment and maintenance of cell polarity in budding yeast. These tools enabled the identification of key molecular components, their mechanisms of action, and the pathway governing the spatiotemporal properties of cell polarity...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28214718/alkyl-substituent-effect-on-photosensitized-inactivation-of-escherichia-coli-by-pyridinium-bonded-p-porphyrins
#20
Jin Matsumoto, Yusaku Suemoto, Hiroki Kanemaru, Kyosuke Takemori, Masato Shigehara, Akari Miyamoto, Haruhiko Yokoi, Masahide Yasuda
Activity of singlet oxygen sensitizers for photoinactivation of bacteria and photodynamic therapy of tumor cells has been evaluated using nonpathogenic model cells, such as Escherichia coli, Saccharomyces cerevisiae, and HeLa cells. Among them, E. coli, gram-negative bacterium, has complex membrane structures in the cell wall, resulting in an impermeable barrier to antimicrobial agents. Therefore, few singlet oxygen sensitizers have photoinactivation activities toward E. coli at low concentrations. Recently polycationic porphyrins have received much attention as a new type of singlet oxygen sensitizers because they have strong binding affinities for DNA and proteins...
February 6, 2017: Journal of Photochemistry and Photobiology. B, Biology
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