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Meiotic recombination

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https://www.readbyqxmd.com/read/28211906/dynamics-of-male-meiotic-recombination-frequency-during-plant-development-using-fluorescent-tagged-lines-in-arabidopsis-thaliana
#1
Fan Li, Nico De Storme, Danny Geelen
Meiotic homologous recombination plays a central role in creating genetic variability, making it an essential biological process relevant to evolution and crop breeding. In this study, we used pollen-specific fluorescent tagged lines (FTLs) to measure male meiotic recombination frequency during the development of Arabidopsis thaliana. Interestingly, a subset of pollen grains consistently shows loss of fluorescence expression in tested lines. Using nine independent FTL intervals, the spatio-temporal dynamics of male recombination frequency was assessed during plant development, considering both shoot type and plant age as independent parameters...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28207814/jmjd-5-kdm8-regulates-h3k36me2-and-is-required-for-late-steps-of-homologous-recombination-and-genome-integrity
#2
Pier Giorgio Amendola, Nico Zaghet, João J Ramalho, Jens Vilstrup Johansen, Mike Boxem, Anna Elisabetta Salcini
The eukaryotic genome is organized in a three-dimensional structure called chromatin, constituted by DNA and associated proteins, the majority of which are histones. Post-translational modifications of histone proteins greatly influence chromatin structure and regulate many DNA-based biological processes. Methylation of lysine 36 of histone 3 (H3K36) is a post-translational modification functionally relevant during early steps of DNA damage repair. Here, we show that the JMJD-5 regulates H3K36 di-methylation and it is required at late stages of double strand break repair mediated by homologous recombination...
February 16, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28205220/comprehensive-genome-profiling-of-single-sperm-cells-by-multiple-annealing-and-looping-based-amplification-cycles-and-next-generation-sequencing-from-carriers-of-robertsonian-translocation
#3
Yanwei Sha, Yankun Sha, Zhiyong Ji, Lu Ding, Qing Zhang, Honggen Ouyang, Shaobin Lin, Xu Wang, Lin Shao, Chong Shi, Ping Li, Yueqiang Song
Robertsonian translocation (RT) is a common cause for male infertility, recurrent pregnancy loss, and birth defects. Studying meiotic recombination in RT-carrier patients helps decipher the mechanism and improve the clinical management of infertility and birth defects caused by RT. Here we present a new method to study spermatogenesis on a single-gamete basis from two RT carriers. By using a combined single-cell whole-genome amplification and sequencing protocol, we comprehensively profiled the chromosomal copy number of 88 single sperms from two RT-carrier patients...
March 2017: Annals of Human Genetics
https://www.readbyqxmd.com/read/28197913/wrestling-with-chromosomes-the-roles-of-sumo-during-meiosis
#4
Amanda C Nottke, Hyun-Min Kim, Monica P Colaiácovo
Meiosis is a specialized form of cell division required for the formation of haploid gametes and therefore is essential for successful sexual reproduction. Various steps are exquisitely coordinated to ensure accurate chromosome segregation during meiosis, thereby promoting the formation of haploid gametes from diploid cells. Recent studies are demonstrating that an important form of regulation during meiosis is exerted by the post-translational protein modification known as sumoylation. Here, we review and discuss the various critical steps of meiosis in which SUMO-mediated regulation has been implicated thus far...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28187145/a-new-sythetic-hybrid-a1d5-between-gossypium-herbaceum-and-g-raimondii-and-its-morphological-cytogenetic-molecular-characterization
#5
Yuxiang Wu, Di Chen, Shuijin Zhu, Lufei Zhang, Lingjiao Li
The diploid species G. herbaceum (A1) and G. raimondii (D5) are the progenitors of allotetraploid cotton, respectively. However, hybrids between G. herbaceum and G. raimondii haven't been reported. In the present study, hybridization between G. herbaceum and G. raimondii was explored. Morphological, cytogenetic and molecular analyses were used to assess the hybridity. The interspecific hybrid plants were successfully obtained. Most of the morphological characteristics of the hybrids were intermediate between G...
2017: PloS One
https://www.readbyqxmd.com/read/28178517/meiotic-knockdown-and-complementation-reveals-essential-role-of-rad51-in-mouse-spermatogenesis
#6
Jieqiong Dai, Oleg Voloshin, Svetlana Potapova, R Daniel Camerini-Otero
Meiotic homologous recombination (HR) is important for proper chromosomal segregation during gametogenesis and facilitates evolutionary adaptation via genomic reshuffling. In most eukaryotes, HR is mediated by two recombinases, the ubiquitous RAD51 and the meiosis-specific DMC1. The role of RAD51 in mammalian meiosis is unclear and study of its function is limited due to embryonic lethality of RAD51 knockouts. Here, we developed an in vivo meiotic knockdown and protein complementation system to study RAD51 during mouse spermatogenesis...
February 7, 2017: Cell Reports
https://www.readbyqxmd.com/read/28155083/the-long-zinc-finger-domain-of-prdm9-forms-a-highly-stable-and-long-lived-complex-with-its-dna-recognition-sequence
#7
Yasmin Striedner, Theresa Schwarz, Thomas Welte, Andreas Futschik, Ulrich Rant, Irene Tiemann-Boege
PR domain containing protein 9 (PRDM9) is a meiosis-specific, multi-domain protein that regulates the location of recombination hotspots by targeting its DNA recognition sequence for double-strand breaks (DSBs). PRDM9 specifically recognizes DNA via its tandem array of zinc fingers (ZnFs), epigenetically marks the local chromatin by its histone methyltransferase activity, and is an important tether that brings the DNA into contact with the recombination initiation machinery. A strong correlation between PRDM9-ZnF variants and specific DNA motifs at recombination hotspots has been reported; however, the binding specificity and kinetics of the ZnF domain are still obscure...
February 2, 2017: Chromosome Research
https://www.readbyqxmd.com/read/28143909/papers-of-note-in-science-355-6323
#8
Annalisa M VanHook
This week's articles include new research about how dengue virus can deplete platelets; a distinct N-end rule pathway for turnover of gluconeogenic enzymes in yeast; and the involvement of SUMO, ubiquitin, and the proteasome in meiotic recombination.
January 31, 2017: Science Signaling
https://www.readbyqxmd.com/read/28142191/partial-preferential-chromosome-pairing-is-genotype-dependent-in-tetraploid-rose
#9
Peter M Bourke, Paul Arens, Roeland E Voorrips, G Danny Esselink, Carole F S Koning-Boucoiran, Wendy P C van 't Westende, Tiago Santos Leonardo, Patrick Wissink, Chaozhi Zheng, Geert van Geest, Richard G F Visser, Frans A Krens, Marinus J M Smulders, Chris Maliepaard
It has long been recognised that polyploid species do not always neatly fall into the categories of auto- or allopolyploid, leading to the term "segmental allopolyploid" to describe everything in between. The meiotic behaviour of such intermediate species is not fully understood, nor is there consensus as to how to model their inheritance patterns. In this study, we used a tetraploid cut rose (Rosa hybrida) population, genotyped using the 68K WagRhSNP array, to construct an ultra-high density linkage map of all homologous chromosomes, using methods previously developed for autotetraploids...
January 31, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28138845/whole-genome-haplotyping-of-single-sperm-of-daphnia-pulex-crustacea-anomopoda
#10
Sen Xu, Kim Young
Sequencing the entire genome of single sperm cells can provide valuable information of the distribution of meiotic recombination events in eukaryotic genomes. Here, we provide a description of the experimental work flow for isolating single sperm cells from the microcrustacean Daphnia pulex using fluorescence-activated cell sorting. Moreover, we describe the application of a whole-genome amplification technique (i.e., Multiple Annealing and Looping Based Amplification Cycles method) to single sperm of Daphnia to generate enough DNA for library preparation of next-generation sequencing...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28138844/haplotyping-a-non-meiotic-diploid-fungal-pathogen-using-induced-aneuploidies-and-snp-cgh-microarray-analysis
#11
Judith Berman, Anja Forche
The generation of haplotype information has recently become very attractive due to its utility for identifying mutations associated with human disease and for the development of personalized medicine. Haplotype information also is crucial for studying recombination mechanisms and genetic diversity, and for analyzing allele-specific gene expression. Classic haplotyping methods require the analysis of hundreds of meiotic progeny. To facilitate haplotyping in the non-meiotic human fungal pathogen Candida albicans, we exploited trisomic heterozygous chromosomes generated via the UAU1 selection strategy...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28138838/haplotyping-of-heterozygous-snps-in-genomic-dna-using-long-range-pcr
#12
Barbara Arbeithuber, Angelika Heissl, Irene Tiemann-Boege
To study meiotic recombination products, cis- or trans-association of disease polymorphisms, or allele-specific expression patterns, it is necessary to phase heterozygous polymorphisms separated by several kilobases. Haplotyping using long-range polymerase chain reaction (PCR) is a powerful, cost-effective method to directly obtain the phase of multiple heterozygous sites with standard laboratory equipment in a handful of loci for many samples. The method is based on the amplification of large genomic DNA regions (up to ~40 kb) with a reaction mixture that combines a proofreading polymerase with allele-specific primer pairs that preferentially amplify matched templates...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28135268/effect-of-mutation-mechanisms-on-variant-composition-and-distribution-in-caenorhabditis-elegans
#13
Ho-Yon Hwang, Jiou Wang
Genetic diversity is maintained by continuing generation and removal of variants. While examining over 800,000 DNA variants in wild isolates of Caenorhabditis elegans, we made a discovery that the proportions of variant types are not constant across the C. elegans genome. The variant proportion is defined as the fraction of a specific variant type (e.g. single nucleotide polymorphism (SNP) or indel) within a broader set of variants (e.g. all variants or all non-SNPs). The proportions of most variant types show a correlation with the recombination rate...
January 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28133604/interaction-of-mre11-and-clinicopathologic-characteristics-in-recurrence-of-breast-cancer-individual-and-cumulated-receiver-operating-characteristic-analyses
#14
Cheng-Hong Yang, Sin-Hua Moi, Li-Yeh Chuang, Shyng-Shiou F Yuan, Ming-Feng Hou, Yi-Chen Lee, Hsueh-Wei Chang
The interaction between the meiotic recombination 11 homolog A (MRE11) oncoprotein and breast cancer recurrence status remains unclear. The aim of this study was to assess the interaction between MRE11 and clinicopathologic variables in breast cancer. A dataset for 254 subjects with breast cancer (220 nonrecurrent and 34 recurrent) was used in individual and cumulated receiver operating characteristic (ROC) analyses of MRE11 and 12 clinicopathologic variables for predicting breast cancer recurrence. In individual ROC analysis, the area under curve (AUC) for each predictor of breast cancer recurrence was smaller than 0...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28122517/evolution-of-meiotic-recombination-genes-in-maize-and-teosinte
#15
Gaganpreet K Sidhu, Tomasz Warzecha, Wojciech P Pawlowski
BACKGROUND: Meiotic recombination is a major source of genetic variation in eukaryotes. The role of recombination in evolution is recognized but little is known about how evolutionary forces affect the recombination pathway itself. Although the recombination pathway is fundamentally conserved across different species, genetic variation in recombination components and outcomes has been observed. Theoretical predictions and empirical studies suggest that changes in the recombination pathway are likely to provide adaptive abilities to populations experiencing directional or strong selection pressures, such as those occurring during species domestication...
January 25, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28118058/smc1%C3%AE-is-required-for-activation-of-sac-during-mouse-oocyte-meiosis
#16
Yilong Miao, Changyin Zhou, Zhaokang Cui, Xiaoxin Dai, Mianqun Zhang, Yajuan Lu, Bo Xiong
Smc1β is a meiosis-specific cohesin subunit that is essential for sister chromatid cohesion and DNA recombination. Previous studies have shown that Smc1β-deficient mice in both sexes are sterile. Ablation of Smc1β during male meiosis leads to the blockage of spermatogenesis in pachytene stage, and ablation of Smc1β during female meiosis generates a highly error-prone oocyte although it could develop to metaphase II stage. However, the underlying mechanisms regarding how Smc1β maintains the correct meiotic progression in mouse oocytes have not been clearly defined...
January 24, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28111257/fragile-sites-dysfunctional-telomere-and-chromosome-fusions-what-is-5s-rdna-role
#17
Alain Victor Barros, Michele Andressa Vier Wolski, Viviane Nogaroto, Mara Cristina Almeida, Orlando Moreira-Filho, Marcelo Ricardo Vicari
Repetitive DNA regions are known as fragile chromosomal sites which present a high flexibility and low stability. Our focus was characterize fragile sites in 5S rDNA regions. The Ancistrus sp. species shows a diploid number of 50 and an indicative Robertsonian fusion at chromosomal pair 1. Two sequences of 5S rDNA were identified: 5S.1 rDNA and 5S.2 rDNA. The first sequence gathers the necessary structures to gene expression and shows a functional secondary structure prediction. Otherwise, the 5S.2 rDNA sequence does not contain the upstream sequences that are required to expression, furthermore its structure prediction reveals a nonfunctional ribosomal RNA...
January 19, 2017: Gene
https://www.readbyqxmd.com/read/28110339/the-effect-of-homoeologous-meiotic-pairing-in-tetraploid-hordeum-bulbosum-l-%C3%A3-h-vulgare-l-hybrids-on-alien-introgressions-in-offspring
#18
Margret Scholz, Galina Pendinen
The pairing behaviour of the individual chromosome arms of Hordeum vulgare (Hv) with their homoeologous arms of H. bulbosum (Hb) at metaphase I of meiosis in tetraploid Hb × Hv hybrids and the frequencies of recombined Hv chromosome arms in selfed offspring were studied on differentially visualized chromosomes after fluorescent in situ hybridisation. The frequencies of paired Hv-Hb arms in the F2 and F3 hybrids were correlated with the frequencies of recombined Hv chromosomes in progenies. Self-generation of hybrids, the number of Hv and Hb chromosomes, and the number of recombined Hv chromosomes of the hybrids strongly influenced the Hv-Hb pairing frequency in meiosis...
2016: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/28108697/understanding-and-manipulating-meiotic-recombination-in-plants
#19
Christophe Lambing, F Christopher H Franklin, Chung-Ju Rachel Wang
No abstract text is available yet for this article.
January 20, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28108587/meiotic-crossing-over-in-maize-knob-heterochromatin
#20
Stephen M Stack, Lindsay A Shearer, Leslie Lohmiller, Lorinda K Anderson
There is ample evidence that crossing over is suppressed in heterochromatin associated with centromeres and nucleolus organizers (NORs). This characteristic has been attributed to all heterochromatin, but the generalization may not be justified. To investigate the relation of crossing over to heterochromatin that is not associated with centromeres or NORs, we used a combination of fluorescence in situ hybridization of the maize 180 bp knob repeat to show the locations of knob heterochromatin and fluorescent immunolocalization of MLH1 protein and AFD1 protein to show the locations of MLH1 foci on maize synaptonemal complexes (SCs ~ pachytene chromosomes)...
January 20, 2017: Genetics
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