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https://www.readbyqxmd.com/read/28210981/assessing-common-bean-cultivars-for-resistance-to-the-soybean-looper-chrysodeixis-includens-lepidoptera-noctuidae
#1
R Morando, E L L Baldin, P L Cruz, A L Lourenção
The soybean looper Chrysodeixis includens (Walker) (Lepidoptera: Noctuidae) is known as an important pest of leguminous plants worldwide. In Brazil, this pest species is gaining importance to producers of the common bean Phaseolus vulgaris L. (Fabaceae) because it limits field production of the crop. Chemical control is still the primary method of insect control. However, due to the possible harmful effects of pesticides to humans and the environment, alternative and less aggressive practices are being investigated...
February 16, 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/28210552/capsular-contracture-in-implant-based-breast-reconstruction-the-effect-of-porcine-acellular-dermal-matrix
#2
Alessia M Lardi, Mark Ho-Asjoe, Klaus Junge, Jian Farhadi
BACKGROUND: Irradiation of implant-based breast reconstructions (BR) is known to increase capsular contracture (CC) rates on average by 4-fold over non-irradiated reconstructions. The use of acellular dermal matrix (ADM) has been associated with lower CC rates in non-irradiated reconstructions (0-3%). Experimental and clinical studies suggest that ADM may also reduce CC rates in irradiated breasts. The aim of this study was to evaluate CC rates in non-irradiated and irradiated one- and two-stage BRs performed with the assistance of porcine ADM (PADM)...
February 2017: Gland Surgery
https://www.readbyqxmd.com/read/28202943/arterial-pressure-lability-is-improved-by-sodium-glucose-cotransporter-2-inhibitor-in-streptozotocin-induced-diabetic-rats
#3
Tomoko Yoshikawa, Takuya Kishi, Keisuke Shinohara, Ko Takesue, Risa Shibata, Noriyuki Sonoda, Toyoshi Inoguchi, Kenji Sunagawa, Hiroyuki Tsutsui, Yoshitaka Hirooka
To prevent cardiovascular events in patients with diabetes mellitus (DM), it is essential to reduce arterial pressure (AP). Sodium-glucose cotransporter 2 inhibitor (SGLT2i) prevents cardiovascular events via the depressor response in patients with DM. In the present study, we examined whether SGLT2i ameliorates AP lability in DM rats. Ten-week-old male Sprague-Dawley rats were administered a single intravenous injection of streptozotocin (50 mg kg(-1)) and were divided into three groups treated with low-dose SGLT2i, vehicle (VEH) or subcutaneously implanted insulin pellets (SGLT2i, VEH and Insulin group, respectively) for 14 days...
February 16, 2017: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/28190668/resilience-correlates-with-outcomes-after-total-shoulder-arthroplasty
#4
John M Tokish, Michael J Kissenberth, Stefan J Tolan, Tariq I Salim, Josh Tadlock, Thomas Kellam, Catherine D Long, Ashley Crawford, Keith T Lonergan, Richard J Hawkins, Ellen Shanley
BACKGROUND: Resilience, characterized by an ability to bounce back or recover from stress, is increasingly recognized as a psychometric property affecting many outcomes' domains including quality of life, suicide risk in active-duty military personnel, and recovery in cancer patients. This study examines the correlation between resilience, as measured by the Brief Resilience Scale (BRS), and traditional outcome scores including the American Shoulder and Elbow Surgeons (ASES), Single Assessment Numeric Evaluation (SANE), and Penn scores in patients undergoing total shoulder arthroplasty (TSA)...
February 10, 2017: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/28184198/local-left-ventricular-epicardial-j-waves-and-late-potentials-in-brugada-syndrome-patients-with-inferolateral-early-repolarization-pattern
#5
Satoshi Nagase, Masamichi Tanaka, Hiroshi Morita, Koji Nakagawa, Tadashi Wada, Masato Murakami, Nobuhiro Nishii, Kazufumi Nakamura, Hiroshi Ito, Tohru Ohe, Kengo F Kusano
Background: Brugada syndrome (BrS) is characterized by J-point or ST-segment elevation on electrocardiograms (ECGs) and increased risk of ventricular fibrillation (VF). In BrS, epicardial depolarization abnormality with delayed potential on the right ventricular outflow tract is reportedly the predominant mechanism underlying VF. Yet VF occurrence is also associated with early repolarization (ER) pattern in the inferolateral ECG leads, which may represent the inferior and/or left lateral ventricular myocardium...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28183560/anesthesia-and-brugada-syndrome-a-12-year-case-series
#6
Mélanie Duque, Luís Santos, Sandy Ribeiro, Dora Catré
STUDY OBJECTIVE: The aim of this 12-year case series was to review the drugs used during anesthetic management of patients with diagnosis of or risk criteria for Brugada syndrome (BrS), and to document any possible association between these drugs and arrhythmogenic activity or unexplained hemodynamic instability. DESIGN: A retrospective clinical observational study. SETTING: Tertiary hospital. PATIENTS: Thirty-one patients met our inclusion criteria: 20 belonging to group D (diagnosed BrS) and 11 to group R (risk of BrS)...
February 2017: Journal of Clinical Anesthesia
https://www.readbyqxmd.com/read/28153259/in-situ-fabrication-of-label-free-optical-sensing-paper-strips-for-the-rapid-surface-enhanced-raman-scattering-sers-detection-of-brassinosteroids-in-plant-tissues
#7
Miao Chen, Zhimin Zhang, Minzhuo Liu, Chen Qiu, Hua Yang, Xiaoqing Chen
A surface-enhanced Raman scattering (SERS)-functionalized, gold nanoparticle (AuNPs)-immobilized paper strip was developed to realize label-free rapid detection of brassinosteroids (BRs) in plant tissues. The AuNPs paper strip was fabricated through utilizing poly(γ-glutamic acid) (γ-PGA) as the linker and concentrator for the directly immobilized AuNPs on paper, which can circumvent many drawbacks of the existing SERS substrates. An enhancement factor of 1.13×10(8), high reproducibility (relative standard deviation of 4...
April 1, 2017: Talanta
https://www.readbyqxmd.com/read/28146157/identification-of-circo-like-virus-brazil-genomic-sequences-in-raw-sewage-from-the-metropolitan-area-of-s%C3%A3-o-paulo-evidence-of-circulation-two-and-three-years-after-the-first-detection
#8
Silvana Beres Castrignano, Teresa Keico Nagasse-Sugahara, Patrícia Garrafa, Telma Alves Monezi, Karina Medici Barrella, Dolores Ursula Mehnert
BACKGROUND: Two novel viruses named circo-like virus-Brazil (CLV-BR) hs1 and hs2 were previously discovered in a Brazilian human fecal sample through metagenomics. CLV-BR hs1 and hs2 possess a small circular DNA genome encoding a replication initiator protein (Rep), and the two genomes exhibit 92% nucleotide identity with each other. Phylogenetic analysis based on the Rep protein showed that CLV-BRs do not cluster with circoviruses, nanoviruses, geminiviruses or cycloviruses. OBJECTIVES: The aim of this study was to search for CLV-BR genomes in sewage and reclaimed water samples from the metropolitan area of São Paulo, Brazil, to verify whether the first detection of these viruses was an isolated finding...
January 19, 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28134094/outcome-after-long-segment-stenting-with-everolimus-eluting-bioresorbable-scaffolds-focusing-on-the-concept-of-overlapping-implantation
#9
Jens Wiebe, Oliver Dörr, Christoph Liebetrau, Timm Bauer, Eva Wilkens, Hanna Ilstad, Niklas Boeder, Albrecht Elsässer, Helge Möllmann, Christian W Hamm, Holger M Nef
INTRODUCTION AND OBJECTIVES: The implantation of bioresorbable scaffolds (BRS) is an emerging technique used in percutaneous coronary interventions. Their application has been extended to more complex lesions, although evidence is only available for simple lesions. The present study evaluated scaffold implantation in long lesions, focusing on overlapping scaffolds. METHODS: We retrospectively analyzed all consecutive patients eligible for stenting with everolimus-eluting poly-L-lactic acid-based BRS with a minimum total scaffold length of 28mm, irrespective of the number of BRS used...
December 2016: Revista Española de Cardiología
https://www.readbyqxmd.com/read/28124256/brassinosteroid-action-in-plant-abiotic-stress-tolerance
#10
Priti Krishna, Bishun D Prasad, Tawhidur Rahman
Brassinosteroids (BRs) are a class of plant steroidal hormones that play essential roles in plant growth and development. Systematic studies had first been undertaken concomitantly to determine both the effects of exogenous BR on stress phenotypes of Arabidopsis thaliana and Brassica napus (rapeseed) seedlings and the expression of stress marker genes in BR-treated and untreated seedlings. When reproducible and convincing evidence of the role of BR in stress tolerance had been obtained, molecular mechanisms underlying the ability of BR to confer tolerance against heat, cold, drought, and salt stress, as well as pathogen resistance were studied with several molecular approaches and tools...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124255/the-primary-root-of-sorghum-bicolor-l-moench-as-a-model-system-to-study-brassinosteroid-signaling-in-crops
#11
David Blasco-Escámez, Fidel Lozano-Elena, Norma Fàbregas, Ana I Caño-Delgado
Roots anchor plants to the soil and are essential for a successful plant growth and adaptation to the environment. Research on the primary root in the plant model system Arabidopsis thaliana has yielded important advances in the molecular and cellular understanding of root growth and development. Several studies have uncovered how the hormones brassinosteroids (BRs) control cell cycle and differentiation programs through different cell-specific signaling pathways that are key for root growth and development...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124254/probing-activation-and-deactivation-of-the-brassinosteroid-insensitive1-receptor-kinase-by-immunoprecipitation
#12
Sara Martins, Grégory Vert, Yvon Jaillais
Brassinosteroids (BRs) are sterol-derived hormones that control plant growth and development. The BR receptor complex is encoded by the BRASSINOSTEROID INSENSITIVE1 (BRI1) and members of the SOMATIC EMBRYOGENESIS RECEPTOR KINASE family. For activation and deactivation, the BR receptor complex uses different posttranslational modifications and recruits various partner proteins. Here, we describe optimized immunoprecipitation protocols and variants for biochemical analyses of posttranslational modifications of BRI1 and of protein-protein interactions...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124247/identification-of-brassinosteroid-target-genes-by-chromatin-immunoprecipitation-followed-by-high-throughput-sequencing-chip-seq-and-rna-sequencing
#13
Trevor Nolan, Sanzhen Liu, Hongqing Guo, Lei Li, Patrick Schnable, Yanhai Yin
Brassinosteroids (BRs) play important roles in many growth and developmental processes. BRs signal to regulate BR-INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1 (BES1) and BRASSINAZOLE-RESISTANT1 (BZR1) transcription factors (TFs), which, in turn, regulate several hundreds of transcription factors (termed BES1/BZR1-targeted TFs or BTFs) and thousands of genes to mediate various BR responses. Chromatin Immunoprecipitation followed by high-throughput sequencing (ChIP-seq) with BES1/BZR1 and BTFs is an important approach to identify BR target genes...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124246/a-technical-framework-for-studying-the-signaling-nexus-of-brassinosteroids-and-immunity
#14
Rosa Lozano-Durán, Youssef Belkhadir
Pathway cross-communication cannot be simply tackled by studying isolated signaling systems. Yet understanding how signal transduction pathways attenuate or reinforce each other in vivo is a challenging task. In plants, biosynthesis and signaling of brassinosteroids (BRs) finely regulate growth and defense programs through a complex array of mechanistic and physiological interactions. Conversely, induction of defenses also impacts on the BR biosynthesis at the transcriptional level. In this chapter, we present an experimental framework to study the physiological connection between BR-controlled growth and defenses...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124245/approaches-to-study-light-effects-on-brassinosteroid-sensitivity
#15
Sandi Paulišić, Maria José Molina-Contreras, Irma Roig-Villanova, Jaime F Martínez-García
Light perception and hormone signaling in plants are likely connected at multiple points. Light conditions, perceived by photoreceptors, control plant responses by altering hormone concentration, tissue sensitivity, or a combination of both. Whereas it is relatively straightforward to assess the light effects on hormone levels, hormone sensitivity is subjected to interpretation. In Arabidopsis thaliana seedlings, hypocotyl length is strongly affected by light conditions. As hypocotyl elongation also depends on brassinosteroids (BRs), assaying this response provides a valuable and easy way to measure the responsiveness of seedlings to BRs and the impact of light...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124242/synthetic-protocol-for-afcs-a-biologically-active-fluorescent-castasterone-analog-conjugated-to-an-alexa-fluor-647-dye
#16
Johan M Winne, Niloufer G Irani, Jos Van den Begin, Annemieke Madder
Synthetic derivatization of hormonally active brassinosteroids (BRs) can provide useful small molecule tools to probe BR signaling pathways, such as fluorescent analogs. However, most biologically active BRs are not suitable for direct chemical conjugation techniques because their derivatization typically requires extensive synthetic work and chemistry expertise. Here, we describe an operationally simple, two-step procedure to prepare and purify an Alexa Fluor 647-castasterone (AFCS) from commercially available materials...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28124241/protocol-for-extraction-and-isolation-of-brassinosteroids-from-plant-tissues
#17
Danuše Tarkowská, Miroslav Strnad
Brassinosteroids (BRs) are a class of steroid plant hormones that participate with other plant hormones in the regulation of numerous developmental processes, including root and shoot growth, vascular differentiation, fertility, and seed germination. A characteristic feature of all plant hormones, including BRs, is that their concentration is extremely low in plant tissues and, therefore, the methods dealing with their determination belong to ultra-trace analysis, for which very sensitive analytical tools are needed...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28114413/visualization-of-bri1-and-serk3-bak1-nanoclusters-in-arabidopsis-roots
#18
Stefan J Hutten, Danny S Hamers, Marije Aan den Toorn, Wilma van Esse, Antsje Nolles, Christoph A Bücherl, Sacco C de Vries, Johannes Hohlbein, Jan Willem Borst
Brassinosteroids (BRs) are plant hormones that are perceived at the plasma membrane (PM) by the ligand binding receptor BRASSINOSTEROID-INSENSITIVE1 (BRI1) and the co-receptor SOMATIC EMBRYOGENESIS RECEPTOR LIKE KINASE 3/BRI1 ASSOCIATED KINASE 1 (SERK3/BAK1). To visualize BRI1-GFP and SERK3/BAK1-mCherry in the plane of the PM, variable-angle epifluorescence microscopy (VAEM) was employed, which allows selective illumination of a thin surface layer. VAEM revealed an inhomogeneous distribution of BRI1-GFP and SERK3/BAK1-mCherry at the PM, which we attribute to the presence of distinct nanoclusters...
2017: PloS One
https://www.readbyqxmd.com/read/28107741/building-related-symptoms-are-linked-to-the-in-vitro-toxicity-of-indoor-dust-and-airborne-microbial-propagules-in-schools-a-cross-sectional-study
#19
J T Salin, M Salkinoja-Salonen, P J Salin, K Nelo, T Holma, P Ohtonen, H Syrjälä
INTRODUCTION: Indoor microbial toxicity is suspected to cause some building-related symptoms, but supporting epidemiological data are lacking. OBJECTIVE: We examined whether the in vitro toxicity of indoor samples from school buildings was associated with work-related health symptoms (building-related symptoms, BRS). METHODS: Administrators of the Helsinki City Real Estate Department selected 15 schools for the study, and a questionnaire on symptoms connected to work was sent to the teachers in the selected schools for voluntary completion...
January 17, 2017: Environmental Research
https://www.readbyqxmd.com/read/28102901/risk-of-arrhythmic-death-in-ischemic-heart-disease-a-prospective-controlled-observer-blind-investigation-over-10-years
#20
Thomas Pezawas, André Diedrich, David Robertson, Robert Winker, Bernhard Richter, Li Wang, Herwig Schmidinger
BACKGROUND: Risk of arrhythmic death is considered highest in ischemic heart disease with severe left ventricular ejection fraction (LVEF) reduction. Non-invasive testing should improve decision-making of prophylactic defibrillator (ICD) implantation. METHODS AND RESULTS: We enrolled 120 patients with ischemic heart disease and LVEF <50% and 30 control subjects without ischemic heart disease and normal LVEF. An initial assessment, a second assessment after 3 years, and a final follow-up comprised of: pharmacological baroreflex testing (BRS), short-term spectral (low frequency (LF) to high frequency (HF) ratio) and long-term time domain analysis of heart rate variability (SDNN), exercise Microvolt T-wave alternans (MTWA) and others...
January 19, 2017: European Journal of Clinical Investigation
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