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https://www.readbyqxmd.com/read/29185955/paraburkholderia-azotifigens-sp-nov-a-nitrogen-fixing-bacterium-isolated-from-paddy-soil
#1
Gyu-Min Choi, Wan-Taek Im
A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strain, designated NF2-5-3T, was isolated from a paddy soil in Anseong city, Republic of Korea. This bacterium was characterized to determine its taxonomic position using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NF2-5-3T had a close relationship with, and was related most closely to, members of the genus Paraburkholderia, namely Paraburkholderia caribensis MWAP64T (98.8 % similarity), P. sabiae Br3407T (98...
November 29, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29165993/hybrid-structured-illumination-expansion-microscopy-reveals-microbial-cytoskeleton-organization
#2
Aaron R Halpern, Germain C M Alas, Tyler J Chozinski, Alexander R Paredez, Joshua C Vaughan
Recently developed tissue-hydrogel methods for specimen expansion now enable researchers to perform super-resolution microscopy with ~65 nm lateral resolution using ordinary microscopes, standard fluores-cent probes, and inexpensive reagents. Here we use the combination of specimen expansion and the optical super-resolution microscopy technique structured illumination microscopy (SIM) to extend the spatial reso-lution to ~30 nm. We apply this hybrid method, which we call ExSIM, to study the cytoskeleton of the im-portant human pathogen Giardia lamblia including the adhesive disc and flagellar axonemes...
November 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/29134929/mucilaginibacter-panaciglaebae-sp-nov-isolated-from-soil-of-a-ginseng-field
#3
Soon Youl Lee, Muhammad Zubair Siddiqi, Sang Yong Kim, Hong Shan Yu, Jae Hak Lee, Wan-Taek Im
A Gram-reaction-negative, strictly aerobic, non-motile and rod-shaped bacterium, designated strain BXN5-31(T), was isolated from soil of a ginseng field, and its taxonomic position was investigated using a polyphasic approach. Strain BXN5-31(T) grew at 18-37 °C and at pH 6.0-8.0 on R2A medium. Based on 16S rRNA gene sequence similarity, strain BXN5-31(T) was shown to belong to the genus Mucilaginibacter and was closely related to Mucilaginibactersoyangensis HME6664(T), Mucilaginibacterximonensis XM-003(T) and Mucilaginibacterpuniceus WS71(T)...
November 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29116033/phenylobacterium-hankyongense-sp-nov-isolated-from-ginseng-field-soil
#4
Gyu-Min Choi, Soon-Youl Lee, Kang Duk Choi, Wan-Taek Im
A Gram-stain-negative, aerobic, non-motile, non-spore-forming and rod-shaped bacterial strain, designated HKS-05(T), was isolated from ginseng field soil. This bacterium was characterized to determine its taxonomic position by using the polyphasic approach. HKS-05(T) grew at 10-37 °C and at pH 6.0-8.0 on R2A agar. On the basis of 16S rRNA gene sequence similarity, HKS-05(T) was shown to represent a member of the family Caulobacteraceaeand to be related to Phenylobacterium lituiforme FaiI3(T) (98.1 % sequence similarity), 'Phenylobacterium zucineum' HLK1 (97...
November 8, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29099722/formation-and-magnetic-properties-of-infep-ag-nano-rods-fabricated-with-noble-metal-ag-using-ion-milling-method
#5
Jae Min Sohn, Hyungsang Kim, Hyunsik Im, Hyungbae Kim, Juwon Lee, Yoon Shon, Ganapathi Subramaniam, Tae Won Kang, Deuk Young Kim, Hyun Cheol Koo, Joo-Hyeon Lee, Jin Dong Song, Chang-Soo Park, Eun Kyu Kim
The formation including density, and height of InFeP:Ag nano-rods doped with noble metal Ag using ion milling method was preponderantly determined from TEM and XRD analyses. We investigate especially the enhanced ferromagnetism of the well aligned InFeP:Ag nano-rods. AES and XPS measurements were carried out in order to investigate the incorporation of Ag and to verify the local chemical bonding of InFeP:Ag nano-rods. The variation of FWHM for DCRC and TAD peaks demonstrates that noble metal Ag is incorporated into InFeP:Ag nano-rods...
November 3, 2017: Nanotechnology
https://www.readbyqxmd.com/read/29090323/vibrio-hannami-sp-nov-isolated-from-seawater
#6
Ga-Eun Lee, Wan-Taek Im, Jin-Sook Park
A Gram-reaction negative, aerobic, motile, non-pigmented and rod-shaped bacterium, designated as 168GH5-2-16(T), was isolated from seawater Jeju island. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Vibrio and was most closely related to Vibrio variabilis R-40492(T) (96.0%). The DNA G+C content was 49.3 mol%. The major polar lipids were phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The predominant quinone was ubiquinone-8 (Q-8)...
October 31, 2017: Current Microbiology
https://www.readbyqxmd.com/read/29039306/paenibacillus-azotifigens-sp-nov-a-novel-nitrogen-fixing-bacterium-isolated-from-paddy-soil
#7
Muhammad Zubair Siddiqi, Gyu-Min Choi, Kang Duk Choi, Wan-Taek Im
A nitrogen-fixing bacterium, designated NF2-4-5(T), was isolated from a paddy soil in Anseong City, Korea. Cells of strain NF2-4-5(T) were Gram-staining-positive, motile rods and aerobic. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Paenibacillus and was closely related to Paenibacillus graminis RSA19(T) (98.7 %), Paenibacillus jilunlii Be17(T) (98.6 %), Paenibacillus salinicaeni LAM0A28(T) (98.6 %) and Paenibacillus riograndensis SBR5(T) (98...
October 17, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28984224/phenylobacterium-deserti-sp-nov-isolated-from-desert-soil
#8
Inam Ullah Khan, Firasat Hussain, Neeli Habib, Mohammed A M Wadaan, Iftikhar Ahmed, Wan-Taek Im, Wael N Hozzein, Xiao-Yang Zhi, Wen-Jun Li
A novel bacterial strain, designated YIM 73061(T), was isolated from the Cholistan desert in Punjab, Pakistan, and characterized by using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Phenylobacterium conjunctum FWC21(T) (97.6 %), Phenylobacterium lituiforme FaiI3(T) (97.4 %), Phenylobacteriumcomposti 4T-6(T) (97.0 %) and Phenylobacterium aquaticum W2-3-4(T) (96.8 %). Cells were Gram-stain-negative, aerobic and motile rods that formed orange colonies...
October 6, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28945539/sphingomonas-agri-sp-nov-a-bacterium-isolated-from-soil
#9
Muhammad Zubair Siddiqi, Gyu-Min Choi, Sang Yong Kim, Kang Duk Choi, Wan-Taek Im
A Gram-reaction-negative, aerobic, non-motile, white (translucent) and rod-shaped bacterium (designated HKS-06T) isolated from soil was characterized by a polyphasic approach to clarify its taxonomic position. Strain HKS-06T was observed to grow optimally at 30 °C and at pH 6.5-7.0 on R2A agar medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HKS-06T belongs to the genus Sphingomonas and is most closely related to Sphingomonas lutea JS5T (97.4 % similarity). The G+C content of the genomic DNA was 64...
September 25, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28821948/mucilaginibacter-ginsenosidivorans-sp-nov-isolated-from-soil-of-ginseng-field
#10
Minseok M Kim, Muhammad Zubair Siddiqi, Wan-Taek Im
A Gram-reaction-negative, aerobic, nonmotile, nonspore-forming, and rod-shaped bacterial strain designated Gsoil 3017(T) was isolated from soil of ginseng field and investigated by phenotypic and phylogenetic analyses. Strain Gsoil 3017(T) grew at 10-37 °C (optimal growth at 30 °C) and at pH 5.5-8.0 (optimal growth at pH 7) on R2A and nutrient agar without additional NaCl as a supplement. Strain Gsoil 3017(T) possessed β-glucosidase activity, which was responsible for its ability to transform ginsenosides Rb1, Rc, and Rd (the three dominant active components of ginseng) to F2 and C-K, respectively...
December 2017: Current Microbiology
https://www.readbyqxmd.com/read/28809138/daejeonia-ginsenosidivorans-gen-nov-sp-nov-a-ginsenoside-transforming-bacterium-isolated-from-lake-water
#11
Muhammad Zubair Siddiqi, Gyu-Min Choi, Mi-Sun Kim, Wan-Taek Im
A Gram-stain-negative, oxidase- and catalase-positive, strictly aerobic, non-gliding bacterial strain, designated as NPT, was isolated from lake water and subjected to a taxonomic study using a polyphasic approach. Colonies of strain NP5T were light-yellow-coloured, circular with regular margins, and opaque. Cells were rods, 0.2-0.5 µm wide and 1.2-3.0 µm long. Strain NP5T possessed β-glycosidase activity, which was responsible for its ability to convert ginsenosides Rb1, Rc and Rd (three main active components of ginseng) to ginsenoside F2...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28752296/pseudaminobacter-granuli-sp-nov-isolated-from-granules-used-in-a-wastewater-treatment-plant
#12
Young Ki Hahn, Minseok S Kim, Wan-Taek Im
A Gram negative, aerobic, non-motile and rod-shaped bacterial strain designated as Gr-2(T) was isolated from granules used in a wastewater treatment plant in Korea, and its taxonomic position was investigated using a polyphasic approach. Strain Gr-2(T) grew at 18-37°C (optimum temperature, 30°C) and a pH of 6.0-8.0 (optimum pH, 7.0) on R2A agar medium. Based on 16S rRNA gene phylogeny, the novel strain showed a new branch within the genus Pseudaminobacter of the family Phyllobacteriaceae, and formed clusters with Pseudaminobacter defluvii THI 051(T) (98...
August 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28699880/paenibacillus-konkukensis-sp-nov-isolated-from-animal-feed
#13
Wan-Taek Im, Kwon-Jung Yi, Sang-Suk Lee, Hyung In Moon, Che Ok Jeon, Dong-Woon Kim, Soo-Ki Kim
A Gram-stain-positive, oxidase- and catalase-positive, aerobic, rod-shaped bacterium, designated strain SK-3146T, was isolated from animal feed. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, revealed that the strain formed a distinct lineage within the genus Paenibacillus that was closely related to Paenibacillusyunnanensis JCM 30953T (98.6 %), Paenibacillusvulneris CCUG 53270T (98.0 %) and Paenibacilluschinjuensis DSM 15045T (96.9 %). Cells were non-motile, endospore-forming and formed milky colonies on NA and R2A agar media...
July 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28689660/structure-of-full-length-smc-and-rearrangements-required-for-chromosome-organization
#14
Marie-Laure Diebold-Durand, Hansol Lee, Laura B Ruiz Avila, Haemin Noh, Ho-Chul Shin, Haeri Im, Florian P Bock, Frank Bürmann, Alexandre Durand, Alrun Basfeld, Sihyun Ham, Jérôme Basquin, Byung-Ha Oh, Stephan Gruber
Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engagement and disengagement to drive SMC action on the chromosome. Here, we elucidate the architecture of prokaryotic Smc dimers by high-throughput cysteine cross-linking and crystallography. Co-alignment of the Smc arms tightly closes the interarm space and misaligns the Smc head domains at the end of the rod by close apposition of their ABC signature motifs...
July 20, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28664515/mucilaginibacter-hankyongensis-sp-nov-isolated-from-soil-of-ginseng-field-baekdu-mountain
#15
Qingmei Liu, Muhammad Zubair Siddiqi, Mi-Sun Kim, Sang Yong Kim, Wan-Taek Im
A Gram-negative, non-motile, aerobic, and rod-shaped bacterial strain designated as BR5-28(T) was isolated from the soil of a ginseng field at Baekdu Mountain Korea, and its taxonomic position was investigated using a polyphasic approach. Strain BR5-28(T) grew at 10-42°C (optimum temperature, 30°C) and pH 5.5-8.5 (optimum pH, 7.0) on R2A agar medium without additional NaCl supplementation. Strain BR5- 28T exhibited β-glucosidase activity, which was responsible for its ability to transform the ginsenosides Rb1 and Rd (the two dominant active components of ginseng) to compound-K...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28640396/an-anatomical-study-of-plate-rod-fixation-in-feline-tibiae
#16
Andreas Gutbrod, Simona Vincenti, Karolin Kühn, Sebastian Christoph Knell, Philipp Alexander Schmierer, Antonio Pozzi
OBJECTIVE: To investigate the feasibility of placing bi-cortical cortex (B-cort) or mono-cortical locking screws (M-lock) in a plate-rod construct applied to the feline tibia in combination with different intramedullary (IM) pins. METHODS: Twenty-four feline tibiae of cats were divided into 4 groups, corresponding to IM pin sizes filling approximately 30% (1.0 mm), 40% (1.4 mm), 45% (1.6 mm), and 50% (1.8 mm) of the medullary canal. Computed tomography (CT) was performed to trace potential screw trajectories in each group...
June 22, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/28638640/crystal-structure-of-n-n-di-decyl-pyromellitic-di-imide
#17
Hansu Im, Myong Yong Choi, Cheol Joo Moon, Tae Ho Kim
The title compound, C30H44N2O4 [systematic name: 2,6-di-decyl-pyrrolo-[3,4-f]iso-indole-1,3,5,7(2H,6H)-tetra-one], consists of a central pyromellitic di-imide moiety with terminal decyl groups at the N-atom positions. The centre of the mol-ecule lies on a crystallographic inversion centre so the asymmetric unit contains one half-mol-ecule. The mol-ecule exhibits a rod-shaped conformation, like other similar compounds of this type, the distance between the ends of terminal decyl groups being 32.45 Å. The packing is dominated by a lamellar arrangement of the mol-ecules, which is reinforced by C-H⋯O hydrogen bonds and C-O⋯π inter-actions, forming a classic herringbone structure...
June 1, 2017: Acta Crystallographica. Section E, Crystallographic Communications
https://www.readbyqxmd.com/read/28357395/new-insights-into-the-function-of-a-versatile-class-of-membrane-molecular-motors-from-studies-of-myxococcus-xanthus-surface-gliding-motility
#18
COMMENT
Tâm Mignot, Marcelo Nöllmann
Cell motility is a central function of living cells, as it empowers colonization of new environmental niches, cooperation, and development of multicellular organisms. This process is achieved by complex yet precise energy-consuming machineries in both eukaryotes and bacteria. Bacteria move on surfaces using extracellular appendages such as flagella and pili but also by a less-understood process called gliding motility. During this process, rod-shaped bacteria move smoothly along their long axis without any visible morphological changes besides occasional bending...
March 2, 2017: Microbial Cell
https://www.readbyqxmd.com/read/28053381/a-simple-method-for-fashioning-an-antibiotic-impregnated-cemented-rod-for-intramedullary-placement-in-infected-non-union-of-long-bones
#19
Ajay Bharti, Umesh Kumar Saroj, Vineet Kumar, Sanjay Kumar, Balram Ji Omar
The intramedullary insertion of antibiotic impregnated PMMA rods, beads or nails are widely used and accepted modality of treatment for chronic osteomyelitis of long bones. But at times it becomes difficult to insert and remove these antibiotic delivery systems owing to narrowed medullary canals. Here we present a technique for preparing PMMA rods with diameter ranging from 6 mm to 3 mm. These rods could easily be placed in narrow intramedullary canal of long bones with chronic osteomyelitis or infected non-union...
October 2016: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/27974091/arachidicoccus-ginsenosidivorans-sp-nov-with-ginsenoside-converting-activity-isolated-from-ginseng-cultivating-soil
#20
Muhammad Zubair Siddiqi, Zubair Aslam, Wan-Taek Im
A Gram-reaction-negative, catalase- and oxidase-positive, aerobic, non-motile, light yellow and rod-shaped bacterium (designated Gsoil 809T) isolated from soil of ginseng field, was characterized by a polyphasic approach to clarify its taxonomic position. Strain Gsoil 809T was observed to grow optimally at 30 °C and at pH 7.0 on nutrient agar medium. Strain Gsoil 809T possessed β-glucosidase activity, which was responsible for its ability to transform protopanaxatriol-type ginsenoside Rg1 to ginsenoside Rh1...
April 2017: International Journal of Systematic and Evolutionary Microbiology
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