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https://www.readbyqxmd.com/read/28821948/mucilaginibacter-ginsenosidivorans-sp-nov-isolated-from-soil-of-ginseng-field
#1
Minseok M Kim, Muhammad Zubair Siddiqi, Wan-Taek Im
A Gram-reaction-negative, aerobic, nonmotile, nonspore-forming, and rod-shaped bacterial strain designated Gsoil 3017(T) was isolated from soil of ginseng field and investigated by phenotypic and phylogenetic analyses. Strain Gsoil 3017(T) grew at 10-37 °C (optimal growth at 30 °C) and at pH 5.5-8.0 (optimal growth at pH 7) on R2A and nutrient agar without additional NaCl as a supplement. Strain Gsoil 3017(T) possessed β-glucosidase activity, which was responsible for its ability to transform ginsenosides Rb1, Rc, and Rd (the three dominant active components of ginseng) to F2 and C-K, respectively...
August 18, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28809138/daejeonia-ginsenosidivorans-gen-nov-sp-nov-a-ginsenoside-transforming-bacterium-isolated-from-lake-water
#2
Muhammad Zubair Siddiqi, Gyu-Min Choi, Mi-Sun Kim, Wan-Taek Im
A Gram-stain-negative, oxidase- and catalase-positive, strictly aerobic, non-gliding bacterial strain, designated as NPT, was isolated from lake water and subjected to a taxonomic study using a polyphasic approach. Colonies of strain NP5T were light-yellow-coloured, circular with regular margins, and opaque. Cells were rods, 0.2-0.5 µm wide and 1.2-3.0 µm long. Strain NP5T possessed β-glycosidase activity, which was responsible for its ability to convert ginsenosides Rb1, Rc and Rd (three main active components of ginseng) to ginsenoside F2...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28752296/pseudaminobacter-granuli-sp-nov-isolated-from-granules-used-in-a-wastewater-treatment-plant
#3
Young Ki Hahn, Minseok S Kim, Wan-Taek Im
A Gram negative, aerobic, non-motile and rod-shaped bacterial strain designated as Gr-2(T) was isolated from granules used in a wastewater treatment plant in Korea, and its taxonomic position was investigated using a polyphasic approach. Strain Gr-2(T) grew at 18-37°C (optimum temperature, 30°C) and a pH of 6.0-8.0 (optimum pH, 7.0) on R2A agar medium. Based on 16S rRNA gene phylogeny, the novel strain showed a new branch within the genus Pseudaminobacter of the family Phyllobacteriaceae, and formed clusters with Pseudaminobacter defluvii THI 051(T) (98...
August 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28699880/paenibacillus-konkukensis-sp-nov-isolated-from-animal-feed
#4
Wan-Taek Im, Kwon-Jung Yi, Sang-Suk Lee, Hyung In Moon, Che Ok Jeon, Dong-Woon Kim, Soo-Ki Kim
A Gram-stain-positive, oxidase- and catalase-positive, aerobic, rod-shaped bacterium, designated strain SK-3146T, was isolated from animal feed. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, revealed that the strain formed a distinct lineage within the genus Paenibacillus that was closely related to Paenibacillusyunnanensis JCM 30953T (98.6 %), Paenibacillusvulneris CCUG 53270T (98.0 %) and Paenibacilluschinjuensis DSM 15045T (96.9 %). Cells were non-motile, endospore-forming and formed milky colonies on NA and R2A agar media...
July 12, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28689660/structure-of-full-length-smc-and-rearrangements-required-for-chromosome-organization
#5
Marie-Laure Diebold-Durand, Hansol Lee, Laura B Ruiz Avila, Haemin Noh, Ho-Chul Shin, Haeri Im, Florian P Bock, Frank Bürmann, Alexandre Durand, Alrun Basfeld, Sihyun Ham, Jérôme Basquin, Byung-Ha Oh, Stephan Gruber
Multi-subunit SMC complexes control chromosome superstructure and promote chromosome disjunction, conceivably by actively translocating along DNA double helices. SMC subunits comprise an ABC ATPase "head" and a "hinge" dimerization domain connected by a 49 nm coiled-coil "arm." The heads undergo ATP-dependent engagement and disengagement to drive SMC action on the chromosome. Here, we elucidate the architecture of prokaryotic Smc dimers by high-throughput cysteine cross-linking and crystallography. Co-alignment of the Smc arms tightly closes the interarm space and misaligns the Smc head domains at the end of the rod by close apposition of their ABC signature motifs...
July 20, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28664515/mucilaginibacter-hankyongensis-sp-nov-isolated-from-soil-of-ginseng-field-baekdu-mountain
#6
Qingmei Liu, Muhammad Zubair Siddiqi, Mi-Sun Kim, Sang Yong Kim, Wan-Taek Im
A Gram-negative, non-motile, aerobic, and rod-shaped bacterial strain designated as BR5-28(T) was isolated from the soil of a ginseng field at Baekdu Mountain Korea, and its taxonomic position was investigated using a polyphasic approach. Strain BR5-28(T) grew at 10-42°C (optimum temperature, 30°C) and pH 5.5-8.5 (optimum pH, 7.0) on R2A agar medium without additional NaCl supplementation. Strain BR5- 28T exhibited β-glucosidase activity, which was responsible for its ability to transform the ginsenosides Rb1 and Rd (the two dominant active components of ginseng) to compound-K...
July 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28640396/an-anatomical-study-of-plate-rod-fixation-in-feline-tibiae
#7
Andreas Gutbrod, Simona Vincenti, Karolin Kühn, Sebastian Christoph Knell, Philipp Alexander Schmierer, Antonio Pozzi
OBJECTIVE: To investigate the feasibility of placing bi-cortical cortex (B-cort) or mono-cortical locking screws (M-lock) in a plate-rod construct applied to the feline tibia in combination with different intramedullary (IM) pins. METHODS: Twenty-four feline tibiae of cats were divided into 4 groups, corresponding to IM pin sizes filling approximately 30% (1.0 mm), 40% (1.4 mm), 45% (1.6 mm), and 50% (1.8 mm) of the medullary canal. Computed tomography (CT) was performed to trace potential screw trajectories in each group...
June 22, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/28638640/crystal-structure-of-n-n-di-decyl-pyromellitic-di-imide
#8
Hansu Im, Myong Yong Choi, Cheol Joo Moon, Tae Ho Kim
The title compound, C30H44N2O4 [systematic name: 2,6-di-decyl-pyrrolo-[3,4-f]iso-indole-1,3,5,7(2H,6H)-tetra-one], consists of a central pyromellitic di-imide moiety with terminal decyl groups at the N-atom positions. The centre of the mol-ecule lies on a crystallographic inversion centre so the asymmetric unit contains one half-mol-ecule. The mol-ecule exhibits a rod-shaped conformation, like other similar compounds of this type, the distance between the ends of terminal decyl groups being 32.45 Å. The packing is dominated by a lamellar arrangement of the mol-ecules, which is reinforced by C-H⋯O hydrogen bonds and C-O⋯π inter-actions, forming a classic herringbone structure...
June 1, 2017: Acta Crystallographica. Section E, Crystallographic Communications
https://www.readbyqxmd.com/read/28357395/new-insights-into-the-function-of-a-versatile-class-of-membrane-molecular-motors-from-studies-of-myxococcus-xanthus-surface-gliding-motility
#9
COMMENT
Tâm Mignot, Marcelo Nöllmann
Cell motility is a central function of living cells, as it empowers colonization of new environmental niches, cooperation, and development of multicellular organisms. This process is achieved by complex yet precise energy-consuming machineries in both eukaryotes and bacteria. Bacteria move on surfaces using extracellular appendages such as flagella and pili but also by a less-understood process called gliding motility. During this process, rod-shaped bacteria move smoothly along their long axis without any visible morphological changes besides occasional bending...
March 2, 2017: Microbial Cell
https://www.readbyqxmd.com/read/28053381/a-simple-method-for-fashioning-an-antibiotic-impregnated-cemented-rod-for-intramedullary-placement-in-infected-non-union-of-long-bones
#10
Ajay Bharti, Umesh Kumar Saroj, Vineet Kumar, Sanjay Kumar, Balram Ji Omar
The intramedullary insertion of antibiotic impregnated PMMA rods, beads or nails are widely used and accepted modality of treatment for chronic osteomyelitis of long bones. But at times it becomes difficult to insert and remove these antibiotic delivery systems owing to narrowed medullary canals. Here we present a technique for preparing PMMA rods with diameter ranging from 6 mm to 3 mm. These rods could easily be placed in narrow intramedullary canal of long bones with chronic osteomyelitis or infected non-union...
October 2016: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/27974091/arachidicoccus-ginsenosidivorans-sp-nov-with-ginsenoside-converting-activity-isolated-from-ginseng-cultivating-soil
#11
Muhammad Zubair Siddiqi, Zubair Aslam, Wan-Taek Im
A Gram-reaction-negative, catalase- and oxidase-positive, aerobic, non-motile, light yellow and rod-shaped bacterium (designated Gsoil 809T) isolated from soil of ginseng field, was characterized by a polyphasic approach to clarify its taxonomic position. Strain Gsoil 809T was observed to grow optimally at 30 °C and at pH 7.0 on nutrient agar medium. Strain Gsoil 809T possessed β-glucosidase activity, which was responsible for its ability to transform protopanaxatriol-type ginsenoside Rg1 to ginsenoside Rh1...
April 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902288/sphingomonas-aquatica-sp-nov-isolated-from-tap-water
#12
Gyu-Min Choi, Jung Hun Jo, Myung-Suk Kang, Minseok S Kim, Soon-Youl Lee, Wan-Taek Im
A Gram-stain-negative, strictly aerobic, rod-shaped bacterium, designated W1-2-1T, was isolated from tap water in South Korea. The strain was characterized by a polyphasic approach to clarify its taxonomic position. Strain W1-2-1T grew at 18-42 °C and at pH 6.0-10.0 on R2A medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Sphingomonas and is most closely related to the Sphingomonas oligophenolica JCM 12082T (97.2 % similarity), Sphingomonas asaccharolyticaNBRC 15499T (96...
April 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27698177/intramedullary-nailing-and-external-ring-fixation-for-tibiotalocalcaneal-arthrodesis-in-charcot-arthropathy
#13
James Richman, Adam Cota, Steven Weinfeld
BACKGROUND: Surgical strategies to address deformities of the ankle and hindfoot in patients with Charcot arthropathy include the use of retrograde intramedullary nails and ring fixators. The literature has not shown superiority of one technique over the other. This study presents a single surgeon's case series of Charcot arthropathy patients treated with either a ring fixator or retrograde intramedullary nail to achieve tibiotalocalcaneal arthrodesis. METHODS: We performed a retrospective analysis of 27 consecutive patients with Charcot arthropathy who underwent a tibiotalocalcaneal arthrodesis using either a retrograde intramedullary (IM) nail (n = 16 patients) or a ring fixator (RF) (n = 11 patients) by a single surgeon...
February 2017: Foot & Ankle International
https://www.readbyqxmd.com/read/27662385/initial-experience-with-percutaneous-im-rodding-of-the-humeri-in-children-with-osteogenesis-imperfecta
#14
Leonid S Grossman, Amber L Price, Eric T Rush, Jennifer L Goodwin, Maegen J Wallace, Paul W Esposito
BACKGROUND: To report a single-center surgical experience treating humeral deformity and fractures in children with osteogenesis imperfecta (OI) using the Fassier-Duval (FD) intramedullary elongating rods. METHODS: A retrospective review was conducted between December 2005 and July 2013 of all OI patients who underwent FD rodding with a minimum of 1-year follow-up. All patients were also being concurrently treated with bisphosphonates. RESULTS: Eighteen patients underwent internal fixation on a total of 35 humeri: 7 males and 11 females with an average age of 49 months...
September 22, 2016: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/27578304/comparison-of-hypersensitivity-rates-to-intravenous-and-intramuscular-peg-asparaginase-in-children-with-acute-lymphoblastic-leukemia-a-meta-analysis-and-systematic-review
#15
REVIEW
Haroon Hasan, Omar Mohammad Shaikh, Shahrad Rod Rassekh, A Fuchsia Howard, Karen Goddard
BACKGROUND: Pegylated-asparaginase (PEG-ASP) is a critical treatment for pediatric acute lymphoblastic leukemia (ALL) and has traditionally been delivered via intramuscular (IM) injection. In an attempt to reduce pain and anxiety, PEG-ASP has increasingly been delivered via intravenous (IV) administration. The study objective was to perform a meta-analysis and systematic review to compare and generate pooled hypersensitivity rates for IM and IV PEG-ASP. METHODS: A systematic literature search was conducted for all epidemiological studies that investigated IV and IM hypersensitivity rates for pediatric ALL...
January 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/27503422/distal-normograde-intramedullary-pin-and-locking-plate-placement-in-the-canine-humerus-a-cadaveric-study
#16
Tim Pearson, Mark Glyde, Giselle Hosgood, Lucas Beierer
OBJECTIVE: To identify a repeatable anatomic landmark for pin insertion and to describe the technique for placement of a distal normograde intramedullary (IM) pin of approximately 35% of the IM diameter using this approach combined with a locking compression plate (LCP) on the medial aspect of the canine humerus. STUDY DESIGN: Ex vivo anatomic study. SAMPLE POPULATION: Canine cadavers (n=10 Greyhounds). METHODS: An anatomic landmark for pin insertion was identified based on three-dimensional reconstructions of previous elbow computed tomography studies and cadaveric dissection of the medial aspect of the humeral condyle...
October 2016: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/27498829/sphingobacterium-jejuense-sp-nov-with-ginsenoside-converting-activity-isolated-from-compost
#17
Muhammad Zubair Siddiqi, Siddiqi Muhammad Shafi, Kang Duk Choi, Wan-Taek Im, Zubair Aslam
A Gram-stain-negative, strictly aerobic, non-motile, light yellow, short-rod bacterium (designated GJ30-7T) isolated from compost, was characterized using a polyphasic approach, in order to clarify its taxonomic position. Strain GJ30-7T was observed to grow optimally at 30 °C and at pH 7.0 on R2A agar medium. Strain Gsoil GJ30-7T possessed β-glucosidase activity, which was responsible for its ability to transform ginsenosides Rb1 and Rc (the two main active components of ginseng) to ginsenoside F2. Phylogenetic analysis, based on 16S rRNA gene sequence similarities, indicated that GJ30-7T belongs to the genus Sphingobacterium of the family Sphingobacteriaceae and was most closely related to Sphingobacterium yanglingense JCM 30166T (92...
November 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27485125/comparison-between-cylindrical-axis-reference-and-articular-surface-reference-femoral-bone-cut-for-total-knee-arthroplasty
#18
Yasuo Niki, Katsuya Nagai, Tomoki Sassa, Kengo Harato, Yasunori Suda
PURPOSE: Reproducing a functional flexion-extension axis (FEA) of the femur is key to achieving successful collateral ligament balance and joint line in total knee arthroplasty (TKA). This study compared the feasibility of cylindrical axis (CA)-reference bone cut and articular surface-reference bone cuts in reproducing the FEA for Japanese osteoarthritis patients. METHODS: The study enrolled 122 knees from 86 patients who underwent primary TKA due to grade III or IV osteoarthritis...
August 2, 2016: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/27412336/panacibacter-ginsenosidivorans-gen-nov-sp-nov-with-ginsenoside-converting-activity-isolated-from-soil-of-a-ginseng-field
#19
Muhammad Zubair Siddiqi, Siddiqi Muhammad Shafi, Kang Duk Choi, Wan-Taek Im
A ginsenoside-transforming bacterium, designated Gsoil 1550T, was isolated from soil of a ginseng field and subjected to a polyphasic taxonomic analysis. Colonies of strain Gsoil 1550T were yellow, of low convexity and with regular margin. Cells were long rods, 0.5-1.2 µm wide and 1.6-3 µm long. The isolate grew at 10-37 °C and at pH 5-9 on R2A agar medium; maximum growth occurred at 30 °C and pH 6-7. Phylogenetic study based on the 16S rRNA gene sequence positioned Gsoil 1550T in a distinct lineage in the family Chitinophagaceae, sharing 92...
October 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27335062/compostibacter-hankyongensis-gen-nov-nbsp-sp-nov-isolated-from-compost
#20
Muhammad Zubair Siddiqi, Siddiqi Muhammad Shafi, Kang Duk Choi, Wan-Taek Im
A novel bacterial strain, designated strain BS27T, was isolated from mushroom compost and subjected to a taxonomic study using a polyphasic approach. Colonies of BS27T were milky-white, circular with regular fringes and opaque. Cells were short rods, 0.3-0.5 µm wide and 1.2-2.0 µm long. Phylogenetic study based on the 16S rRNA gene sequence placed BS27T in a distinct lineage in the family Chitinophagaceae, sharing 90.1-90.9 % sequence similarity with members of the closely related genera Chitinophaga, Flavitalea, Flavihumibacter, Lacibacter and Flavisolibacter...
September 2016: International Journal of Systematic and Evolutionary Microbiology
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