Chen He, Ke Xu, Xiaoyan Zhu, Paige S Dunphy, Brian Gudenas, Wenwei Lin, Nathaniel Twarog, Laura D Hover, Chang-Hyuk Kwon, Lawryn H Kasper, Junyuan Zhang, Xiaoyu Li, James Dalton, Barbara Jonchere, Kimberly S Mercer, Duane G Currier, William Caufield, Yingzhe Wang, Jia Xie, Alberto Broniscer, Cynthia Wetmore, Santhosh A Upadhyaya, Ibrahim Qaddoumi, Paul Klimo, Frederick Boop, Amar Gajjar, Jinghui Zhang, Brent A Orr, Giles W Robinson, Michelle Monje, Burgess B Freeman Iii, Martine F Roussel, Paul A Northcott, Taosheng Chen, Zoran Rankovic, Gang Wu, Jason Chiang, Christopher L Tinkle, Anang A Shelat, Suzanne J Baker
Pediatric high-grade glioma (pHGG) is a major contributor to cancer-related death in children. In vitro and in vivo disease models reflecting the intimate connection between developmental context and pathogenesis of pHGG are essential to advance understanding and identify therapeutic vulnerabilities. Here we report establishment of 21 patient-derived pHGG orthotopic xenograft (PDOX) models and eight matched cell lines from diverse groups of pHGG. These models recapitulate histopathology, DNA methylation signatures, mutations and gene expression patterns of the patient tumors from which they were derived, and include rare subgroups not well-represented by existing models...
July 2, 2021: Nature Communications