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Tripti Pandey, Rohit Shukla, Harish Shukla, Amit Sonkar, Timir Tripathi, Arvind Kumar Singh
Peroxides are one of the most important radicals that cause oxidative stress. Certain Glutathione S-transferases (GSTs) have been reported to show peroxidase activity. We report a novel peroxidase activity of Synechocystis GST- sll1545. The recombinant protein was purified to homogeneity and characterized. Low Km (0.109μM) and high Vmax (0.663μmol.min(-1)) values suggest a high preference of sll1545 for cumenehydroperoxide. Disc inhibition assay confirmed the ability of the enzyme to protect cells against peroxide-induced damage...
October 16, 2016: International Journal of Biological Macromolecules
Naoyuki Makita, Jun Ishiguro, Keisuke Suzuki, Futoshi Nara
OBJECTIVES: Recently, there has been a growing interest in the mechanism of action of dichloroacetate (DCA) for T-cell differentiation; however, this mechanism has not been elucidated in detail. Therefore, this study aimed to investigate the mechanism of action of DCA for Treg and Th17 differentiation with pyruvate dehydrogenase kinase (PDHK) inhibitor (AZD7545) and PDHK knockdown. METHODS: Inhibitory activity of DCA and AZD7545 against recombinant PDHK and intracellular PDH phosphorylation was measured...
October 19, 2016: Journal of Pharmacy and Pharmacology
Tatjana Harting, Mandy Stubbendorff, Saskia Willenbrock, Siegfried Wagner, Patrik Schadzek, Anaclet Ngezahayo, Hugo Murua Escobar, Ingo Nolte
The Warburg effect describes the ability of cancer cells to produce energy via aerobic glycolysis instead of oxidative phosphorylation of pyruvate. This deviation in mitochondrial metabolism inhibits apoptosis, allowing increased proliferation under conditions of reduced oxygen levels. Dichloroacetate (DCA) was successfully used in several human cancer cell lines to reactivate oxidative phosphorylation in mitochondria. The aim of this study was the characterization and response of canine cancer cell lines after DCA exposure...
October 5, 2016: International Journal of Oncology
Lina Yao, Hui Shen, Nan Wang, Jaspaul Tatlay, Liang Li, Tin Wee Tan, Yuan Kun Lee
Microalgal neutral lipids (mainly in the form of triacylglycerols (TAGs)), feasible substrates for biofuel, are typically accumulated during the stationary growth phase. To make microalgal biofuels economically competitive with fossil fuels, generating strains that trigger TAG accumulation from the exponential growth phase is a promising biological approach. The regulatory mechanisms to trigger TAG accumulation from the exponential growth phase (TAEP) are important to be uncovered for advancing economic feasibility...
October 13, 2016: Plant Biotechnology Journal
Paula Ribao, Maria J Rivero, Inmaculada Ortiz
Noble metals have been used to improve the photocatalytic activity of TiO2. Noble metal nanoparticles prevent charge recombination, facilitating electron transport due to the equilibration of the Fermi levels. Furthermore, noble metal nanoparticles show an absorption band in the visible region due to a high localized surface plasmon resonance (LSPR) effect, which contributes to additional electron movements. Moreover, systems based on graphene, titanium dioxide, and noble metals have been used, considering that graphene sheets can carry charges, thereby reducing electron-hole recombination, and can be used as substrates of atomic thickness...
October 11, 2016: Environmental Science and Pollution Research International
O N Pyaskovskaya, D L Kolesnik, A G Fedorchuk, I V Prokhorova, G I Solyanik
UNLABELLED: Aerobic glycolysis that supports high proliferation rate and survival of tumor cells in unfavorable conditions is among fundamental features of tumor metabolism. The search for active modulators of energetic metabolism capable of suppressing tumor growth and metastasis could result in higher effectiveness of anticancer therapy. AIM: To study antitumor and antimetastatic activity of the modulators of energetic metabolism dichloroacetate (DCA) and 2-deoxy-D-glucose (2DG) used in combination treatment of Lewis lung carcinoma (LLC)...
September 2016: Experimental Oncology
Yu-Ting Zuo, Yu Hu, Wei-Wei Lu, Jing-Jing Cao, Fan Wang, Xue Han, Wen-Qing Lu, Ai-Lin Liu
Scarce toxicological data are available for 2,6-dichloro-1,4-benzoquinone (DCBQ), an emerging water disinfection by-product (DBP) that is of potential public health concern. This study investigated the effects of DCBQ on the lethality, respiration rate, and DNA damage in the Caenorhabditis elegans nematode. Meanwhile, the toxic effects of five regulated DBPs, dichloroacetic acid (DCA), trichloroacetic acid (TCA), monobromoacetic acid (MBA), dibromoacetic acid (DBA), and N-nitrosodimethylamine (NDMA), have also been evaluated...
September 17, 2016: Journal of Hazardous Materials
Xianbin Su, Ruihong Li, Ka-Fai Kong, Jimmy S H Tsang
Haloacids are considered to be environmental pollutants, but some of them have also been tested in clinical research. The way that haloacids are transported across biological membranes is important for both biodegradation and drug delivery purposes. In this review, we will first summarize putative haloacids transporters and the information about haloacids transport when studying carboxylates transporters. We will then introduce MCT1 and SLC5A8, which are respective transporter for antitumor agent 3-bromopyruvic acid and dichloroacetic acid, and monochloroacetic acid transporters Deh4p and Dehp2 from a haloacids-degrading bacterium...
September 23, 2016: Biochimica et Biophysica Acta
Jinhui Pang, Xin Liu, Jun Yang, Fachuang Lu, Bo Wang, Feng Xu, Mingguo Ma, Xueming Zhang
In the present study, we describe a novel one-step method to prepare water-soluble cellulose acetate (WSCA) with higher degree of polymerization values (DP = 650-680) by in situ activation of carboxyl group in ionic liquid. First of all, cellulose was dissolved in 1-ethyl-3-methylimidazolium acetate (EmimAc) and reacted with dichloroacetyl chloride (Cl2AcCl) in order to make cellulose dichloroacetate. Under various conditions, a series of water soluble products were produced. Elemental analysis and NMR results confirmed that they were cellulose acetate with DS (degree of substitution) values in the range from 0...
2016: Scientific Reports
Gaochao Dong, Qiang Chen, Feng Jiang, Decai Yu, Qixing Mao, Wenjie Xia, Run Shi, Jie Wang, Lin Xu
Radiotherapy is generally applied in the treatment of esophageal squamous cell carcinoma (ESCC). However, the radioresistance of ESCC still remains an obstacle for the curative effect of this treatment. We hypothesized that diisopropylamine dichloroacetate (DADA), an inhibitor of pyruvate dehydrogenase kinase (PDK), might enhance radiosensitizationin resistant ESCC. The clonogenic survival assay revealed that DADA sensitized ESCC cells to radiotherapy in vitro; furthermore, the combination of DADA and radiotherapy increased the expression of γ-H2AX, which is a hallmark of DNA double-strand breaks...
September 8, 2016: Oncotarget
Emanuele Moccia, Adriano Intiso, Angela Cicatelli, Antonio Proto, Francesco Guarino, Patrizia Iannece, Stefano Castiglione, Federico Rossi
Trichloroethylene (TCE) is a chlorinated aliphatic organic compound often detected as pollutant in soils and ground water. "Green technologies" based on phytoremediation were proven to be effective to reclaim organic pollutants (e.g. TCE) and heavy metals from different environmental matrices. In this work, we use Zea mays L. for the removal of high TCE concentrations from medium cultures. In particular, we investigated a sealed bioreactor where the growth medium was contaminated with an increasing amount of TCE, in the range 55-280 mg/L; the removal capability of the maize plants was assessed by means of GC-MS and LC-MS analyses...
September 13, 2016: Environmental Science and Pollution Research International
Luminita Andronic, Luminita Isac, Sara Miralles-Cuevas, Maria Visa, Isabel Oller, Anca Duta, Sixto Malato
Materials with photocatalytic and adsorption properties for advanced wastewater treatment targeting reuse were studied. Making use of TiO2 as a well-known photocatalyst, Cu2S as a Vis-active semiconductor, and fly ash as a good adsorbent, dispersed mixtures/composites were prepared to remove pollutants from wastewater. X-ray diffraction, scanning electron microscopy, energy-dispersive X-Ray spectroscopy, atomic force microscopy, band gap energy, point of zero charge (pHpzc) and BET porosity were used to characterize the substrates...
December 15, 2016: Journal of Hazardous Materials
Lei Su, Hailin Zhang, Chen Yan, Aiping Chen, Gang Meng, Jiwu Wei, Decai Yu, Yitao Ding
Dichloroacetate (DCA), an inhibitor of pyruvate dehydrogenase kinase, has anti-tumor properties in various carcinoma models. Diisopropylamine dichloroacetate (DADA), an over-the-counter drug for chronic liver disease, is a derivative of DCA. To date, few studies have evaluated the anticancer potential of DADA in breast cancer. In this study, MDA-MB-231 cells, a breast adenocarcinoma cell line, were used in in vitro and in vivo experiments to evaluate the anti-tumor efficacy of DADA and DCA. The half maximal inhibitory concentration (IC50) of DADA (7...
August 25, 2016: Oncotarget
Ianis Delpla, Manuel J Rodriguez
Spring rainfall events can have deleterious impacts on raw and drinking water quality for water treatment plants that use surface waters. This study compares the influence of land use and climate on DBP precursors in two catchments supplying the region around the City of Québec, Canada, and assesses the variability of Disinfection By-Product (DBP) concentration and speciation following rainfall events. DBPs (trihalomethanes (THMs) and haloacetic acids (HAAs)) and their precursors in raw waters (pH, turbidity, specific ultraviolet absorbance (SUVA), total and dissolved organic carbon, bromides and chlorine dose) were monitored...
November 1, 2016: Water Research
Hiroshi Kido, Irene L Indalao, Hyejin Kim, Takashi Kimoto, Satoko Sakai, Etsuhisa Takahashi
Severe influenza is characterized by cytokine storm and multiorgan failure. Influenza patients with underlying diseases show a rapid progression in disease severity. The major mechanism that underlies multiorgan failure during the progressive stage of infection, particularly in patients with underlying risk factors, is mitochondrial energy crisis. The relationship between the factors that determine infection severity, such as influenza virus, cytokines, cellular trypsin as a hemagglutinin processing protease for viral multiplication, accumulation of metabolic intermediates and ATP crisis in mitochondria, is termed the "influenza virus-cytokine-trypsin" cycle...
September 2016: Respiratory Investigation
Susan D Hester, Virunya Bhat, Brian N Chorley, Gleta Carswell, Wendell Jones, Leah C Wehmas, Charles E Wood
Use of archival resources has been limited to date by inconsistent methods for genomic profiling of degraded RNA from formalin-fixed paraffin-embedded (FFPE) samples. RNA-sequencing offers a promising way to address this problem. Here we evaluated transcriptomic dose responses using RNA-sequencing in paired FFPE and frozen (FROZ) samples from two archival studies in mice, one <2 years old and the other >20 years old. Experimental treatments included 3 different doses of di(2-ethylhexyl)phthalate or dichloroacetic acid for the recently archived and older studies, respectively...
August 25, 2016: Toxicological Sciences: An Official Journal of the Society of Toxicology
Rafael Moreno-Sánchez, Álvaro Marín-Hernández, Isis Del Mazo-Monsalvo, Emma Saavedra, Sara Rodríguez-Enríquez
BACKGROUND: Exceedingly high therapeutic/experimental doses of metabolic drugs such as oxamate, aminooxyacetate (AOA) and dichloroacetate (DCA) are required to diminish growth, glycolysis and oxidative phosphorylation (OxPhos) of different cancer cells. To identify the mechanisms of action of these drugs on cancer energy metabolism, a systematic analysis of their specificities was undertaken. METHODS: Hepatocarcinoma AS-30D cells were treated with the inhibitors and glycolysis and OxPhos enzyme activities, metabolites and fluxes were analyzed...
August 16, 2016: Biochimica et Biophysica Acta
J Michael Wright, Amanda Evans, John A Kaufman, Zorimar Rivera-Núñez, Michael G Narotsky
BACKGROUND: Epidemiological studies suggest that women exposed to disinfection by-products (DBPs) have an increased risk of delivering babies with cardiovascular defects (CVDs). OBJECTIVE: We examined nine CVDs in relation to categorical DBP exposures including bromoform, chloroform, dibromochloromethane (DBCM), bromodichloromethane (BDCM), monobromoacetic acid (MBAA), dichloroacetic acid (DCAA), trichloroacetic acid (TCAA), and summary DBP measures (HAA5, THMBr, THM4, and DBP9)...
August 12, 2016: Environmental Health Perspectives
Zheng Yang, Kin Y Tam
Glycolysis has been observed as a predominant process for most cancer cells to utilize glucose, which was referred to as "Warburg Effect". Targeting critical enzymes, such as pyruvate dehydrogenase kinase (PDK) that inversely regulating the process of glycolysis could be a promising approach to work alone or in combination with other treatments for cancer therapy. EGFR inhibitors for Non-Small-Cell Lung Cancer (NSCLC) treatment have been applied for decades in clinical practices with great success, but also their clinical benefits were somewhat hampered by the rising acquired-resistance...
October 15, 2016: European Journal of Pharmacology
X D Liu, W Zhao, X F Pan, H F Yan
OBJECTIVE: To establish a SPE-IC method to determine the thiodiglycolic acid in the urine of workers who were exposed to vinyl chloride monomer. METHODS: The samples were prepared by the process of being centrifuged and purified by C18 SPE column before separated by AS19 anion exchange column and detected by conductivity detector. The thiodiglycolic acid were characterized by the retention time and quantified by peak area and external standard method. RESULTS: The range of linearity was 0...
April 20, 2016: Chinese Journal of Industrial Hygiene and Occupational Diseases
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