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Cytochrome P

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https://www.readbyqxmd.com/read/28822867/de-novo-assembly-and-characterization-of-seabass-lateolabrax-japonicus-transcriptome-and-expression-of-hepatic-genes-following-different-dietary-phosphorus-calcium-levels
#1
Kang-Le Lu, Zhong-Li Ji, Samad Rahimnejad, Chun-Xiao Zhang, Ling Wang, Kai Song
Fish farming seriously influences the aquatic environment because most dietary phosphorus (P) is excreted in the effluent. To increase the P utilization in fish, molecular techniques should be explored given the remarkable development of these techniques. Thus, to identify the candidate genes related to P utilization and molecular alterations following administration of a P-deficient diet in seabass Lateolabrax japonicus, we assessed the de novo pituitary, gill, intestine, liver, kidney, scales and vertebra transcriptomes, and we compared the expression of hepatic genes with three diets varying in P and Ca levels: diet I (0...
August 9, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28822193/-effect-of-ligustri-lucidi-fructus-on-hepatic-cox-activity-in-normal-rats-and-preliminary-investigation-of-its-dna-methylation-mechanism
#2
Chao Wu, Wei-Feng Zhu, Qin Ji, Ying Zhou, Ming Zhang, Xin Huang, Shu-Hong Peng
The study aims to investigate the effect of Ligustri Lucidi Fructus on cytochrome c oxidase(COX) activity in rat hepatic tissues and explore its possible mechanism of DNA methylation. Male SD rats received aqueous extract of Ligustri Lucidi Fructus (2.0,6.0 g•kg⁻¹) by intragastric administration for 30 d. After the rats were sacrificed, hepatic tissues of rats were taken to detect COX activity, protein concentration of COX4I1, TET1 and DNMT3A protein levels, mRNA expression levels of Cox4i1, Dnmt3a and Tet1, and determine the DNA methylation frequency of Cox4i1...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28821467/in-vitro-characterization-of-cyp102g4-from-streptomyces-cattleya-a-self-sufficient-p450-naturally-producing-indigo
#3
Joonwon Kim, Pyung-Gang Lee, Eun-Ok Jung, Byung-Gee Kim
Self-sufficient CYP102As possess outstanding hydroxylating activity to fatty acids such as myristic acid. Other CYP102 subfamily members share substrate specificity of CYP102As, but, occasionally, unusual characteristics of its own subfamily have been found. In this study, only one self-sufficient cytochrome P450 from Streptomyces cattleya was renamed from CYP102A_scat to CYP102G4, purified and characterized. UV-Vis spectrometry pattern, FAD/FMN analysis, and protein sequence comparison among CYP102s have shown that CYP102 from Streptomyces cattleya belongs to CYP102G subfamily...
August 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28820887/identification-of-putative-qtls-for-seedling-stage-phosphorus-starvation-response-in-finger-millet-eleusine-coracana-l-gaertn-by-association-mapping-and-cross-species-synteny-analysis
#4
M Ramakrishnan, S Antony Ceasar, K K Vinod, V Duraipandiyan, T P Ajeesh Krishna, Hari D Upadhyaya, N A Al-Dhabi, S Ignacimuthu
A germplasm assembly of 128 finger millet genotypes from 18 countries was evaluated for seedling-stage phosphorus (P) responses by growing them in P sufficient (Psuf) and P deficient (Pdef) treatments. Majority of the genotypes showed adaptive responses to low P condition. Based on phenotype behaviour using the best linear unbiased predictors for each trait, genotypes were classified into, P responsive, low P tolerant and P non-responsive types. Based on the overall phenotype performance under Pdef, 10 genotypes were identified as low P tolerants...
2017: PloS One
https://www.readbyqxmd.com/read/28818492/-dexmedetomidine-preconditioning-protects-against-lipopolysaccharides-induced-injury-in-the-human-alveolar-epithelial-cells
#5
Lei Zhang, Xian-Jin Zhou, Li-Ying Zhan, Xiao-Jing Wu, Wen-Lan Li, Bo Zhao, Qing-Tao Meng, Zhong-Yuan Xia
BACKGROUND AND OBJECTIVES: Dexmedetomidine (DEX) has demonstrated the preconditioning effect and shown protective effects against organize injury. In this study, using A549 (human alveolar epithelial cell) cell lines, we investigated whether DEX preconditioning protected against acute lung injury (ALI) in vitro. METHODS: A549 were randomly divided into four groups (n=5): control group, DEX group, lipopolysaccharides (LPS) group, and D-LPS (DEX+LPS) group. Phosphate buffer saline (PBS) or DEX were administered...
August 14, 2017: Revista Brasileira de Anestesiologia
https://www.readbyqxmd.com/read/28814372/association-of-cyp19a1-gene-polymorphisms-with-anoestrus-in-water-buffaloes
#6
Khairy M El-Bayomi, Ayman A Saleh, Ashraf Awad, Mahmoud S El-Tarabany, Hadeel S El-Qaliouby, Mohamed Afifi, Shymaa El-Komy, Walaa M Essawi, Essam A Almadaly, Mohammed A El-Magd
Cytochrome P450 aromatase (encoded by the CYP19A1 gene) regulates oestrogen biosynthesis and so plays an essential role in female fertility. We investigated the genetic association of CYP19A1 with the risk of anoestrus in Egyptian water buffaloes. A total of 651 animals (326 anoestrous and 325 cycling) were used in this case-control study. Using single-strand conformation polymorphisms and sequencing, four single nucleotide polymorphisms (SNPs) were detected; c.-135T>C SNP in the 5'UTR and three non-synonymous SNPs: c...
August 17, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28813209/galangin-a-natural-flavonoid-reduces-mitochondrial-oxidative-damage-in-streptozotocin-induced-diabetic-rats
#7
Amal A Aloud, Chinnadurai Veeramani, Chandramohan Govindasamy, Mohammed A Alsaif, Khalid S Al-Numair
OBJECTIVE: We designed this study to observe the effect of galangin on damaged mitochondria in the liver of diabetic rats. METHODS: Male albino Wistar rats were made diabetic by injecting streptozotocin (STZ) intraperitoneally (40 mg kg(-1) body weight (BW)). Galangin (8 mg kg(-1) BW) or glibenclamide (600 µg kg(-1) BW) was given orally daily once for 45 days to both healthy and diabetic rats. RESULTS: Diabetic rats showed significant (P < 0...
August 16, 2017: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/28812950/expression-of-cytochrome-p450-regulators-in-cynomolgus-macaque
#8
Yasuhiro Uno, Hiroshi Yamazaki
1. Cytochrome P450 (P450) regulators including nuclear receptors and transcription factors have not been fully investigated in cynomolgus macaques, an important species used in drug metabolism studies. In this study, we analyzed seventeen P450 regulators by sequence and phylogenetic analysis, and tissue expression. 2. Gene and genome structures of seventeen P450 regulators were similar to the human orthologs, and the deduced amino acid sequences showed high sequence identities (92-95%) and more closely clustered, with the human orthologs...
August 16, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/28812436/the-mechanism-of-protective-effect-of-crocin-against-liver-mitochondrial-toxicity-caused-by-arsenic-iii
#9
Bahareh Sadat Yousefsani, Jalal Pourahmad, Hossein Hosseinzadeh
In this study, we want to understand whether crocin could prevent mitochondrial damage caused by As III. For this purpose, we determined different mitochondrial toxicity endpoints caused by As III. We evaluated mitochondrial ROS formation, lipid peroxidation, mitochondrial membrane potential collapse, mitochondrial outer membrane integrity and cytochrome c release. Our results showed that pretreatment with crocin at a concentration of 25 µg/ml significantly (P < 0.001) reduced As III induced mitochondrial ROS formation, lipid peroxidation, mitochondrial membrane potential collapse and mitochondrial swelling...
August 16, 2017: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/28808420/inhibition-of-mtor-signaling-confers-protection-against-cerebral-ischemic-injury-in-acute-hyperglycemic-rats
#10
Changchun Hei, Ping Liu, Xiao Yang, Jianguo Niu, P Andy Li
Hyperglycemia is known to exacerbate neuronal death resulted from cerebral ischemia. The mechanisms are not fully understood. The mammalian target of rapamycin (mTOR) pathway regulates cell growth, division and apoptosis. Recent studies suggest that activation of mTOR may mediate ischemic brain damage. The objective of the present experiment is to explore whether mTOR mediates ischemic brain damage in acute hyperglycemic animals. Rats were subjected to 10 min of forebrain ischemia under euglycemic, hyperglycemic and rapamycin-treated hyperglycemic conditions...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28808417/down-regulation-of-aminopeptidase-n-and-abc-transporter-subfamily-g-transcripts-in-cry1ab-and-cry1ac-resistant-asian-corn-borer-ostrinia-furnacalis-lepidoptera-crambidae
#11
Tiantao Zhang, Brad S Coates, Yueqin Wang, Yidong Wang, Shuxiong Bai, Zhenying Wang, Kanglai He
The Asian corn borer (ACB), Ostrinia furnacalis (Lepidoptera: Crambidae), is a highly destructive pest of cultivated maize throughout East Asia. Bacillus thuringiensis (Bt) crystalline protein (Cry) toxins cause mortality by a mechanism involving pore formation or signal transduction following toxin binding to receptors along the midgut lumen of susceptible insects, but this mechanism and mutations therein that lead to resistance are not fully understood. In the current study, quantitative comparisons were made among midgut expressed transcripts from O...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28807731/time-resolved-generation-of-membrane-potential-by-ba3-cytochrome-c-oxidase-from-thermus-thermophilus-coupled-to-single-electron-injection-into-the-o-and-oh-states
#12
Sergey A Siletsky, Ilya Belevich, Nikolay P Belevich, Tewfik Soulimane, Mårten Wikström
Two electrogenic phases with characteristic times of ~14μs and ~290μs are resolved in the kinetics of membrane potential generation coupled to single-electron reduction of the oxidized "relaxed" O state of ba3 oxidase from T. thermophilus (O→E transition). The rapid phase reflects electron redistribution between CuA and heme b. The slow phase includes electron redistribution from both CuA and heme b to heme a3, and electrogenic proton transfer coupled to reduction of heme a3. The distance of proton translocation corresponds to uptake of a proton from the inner water phase into the binuclear center where heme a3 is reduced, but there is no proton pumping and no reduction of CuB...
August 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28804552/in-vivo-opening-of-the-mitochondrial-permeability-transition-pore-in-a-rat-model-of-ventricular-fibrillation-and-closed-chest-resuscitation
#13
Iyad M Ayoub, Jeejabai Radhakrishnan, Raúl J Gazmuri
Opening of the mitochondrial permeability transition pore (mPTP) is considered central to reperfusion injury. Yet, most of our knowledge comes from observations in isolated mitochondria, cells, and organs. We used a rat model of ventricular fibrillation (VF) and closed-chest resuscitation to examine whether the mPTP opens in vivo and whether cyclosporine A (CsA) attenuates the associated myocardial injury. Two series of 26 and 18 rats each underwent 10 minutes of untreated VF before attempting resuscitation...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28803810/autophagy-plays-a-positive-role-in-zinc-induced-apoptosis-in-intestinal-porcine-epithelial-cells
#14
Xin Zhou, Yansen Li, ChunMei Li
A high‑zinc (Zn) diet has been found to promote growth performance in pigs, but causes cell apoptosis in the intestinal epithelium. To investigate the mechanism underlying the high dietary Zn-induced apoptosis in porcine small intestinal epithelium, IPEC-J2 cells (intestinal porcine epithelial cells) were used to determine the effect of Zn on cell inhibitory, apoptosis, and mitochondrial membrane potential and autophagy in vitro. Results showed that Zn inhibited IPEC-J2 cell proliferation and induced apoptosis and autophagy...
August 10, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28803751/insulin-prevents-aberrant-mitochondrial-phenotype-in-sensory-neurons-of-type-1-diabetic-rats
#15
Mohamad-Reza Aghanoori, Darrell R Smith, Subir Roy Chowdhury, Mohammad Golam Sabbir, Nigel A Calcutt, Paul Fernyhough
Diabetic neuropathy affects approximately 50% of diabetic patients. Down-regulation of mitochondrial gene expression and function has been reported in both human tissues and in dorsal root ganglia (DRG) from animal models of type 1 and type 2 diabetes. We hypothesized that loss of direct insulin signaling in diabetes contributes to loss of mitochondrial function in DRG neurons and to development of neuropathy. Sensory neurons obtained from age-matched adult control or streptozotocin (STZ)-induced type 1 diabetic rats were cultured with or without insulin before determining mitochondrial respiration and expression of mitochondrial respiratory chain and insulin signaling-linked proteins...
August 10, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28803388/genetic-diversity-of-the-human-head-lice-pediculus-humanus-capitis-among-primary-school-girls-in-saudi-arabia-with-reference-to-their-prevalence
#16
Sarah A Al-Shahrani, Reem A Alajmi, Tahany H Ayaad, Mohammed A Al-Shahrani, El-Sayed H Shaurub
The present work aimed at investigating the genetic diversity of the head louse Pediculus humanus capitis (P. humanus capitis) among infested primary school girls at Bisha governorate, Saudi Arabia, based on the sequence of mitochondrial cytochrome b (mt cyt b) gene of 121 P. humanus capitis adults. Additionally, the prevalence of pediculosis capitis was surveyed. The results of sequencing were compared with the sequence of human head lice that are genotyped previously. Phylogenetic tree analysis showed the presence of 100% identity (n = 26) of louse specimens with clade A (prevalent worldwide) of the GenBank data base...
August 13, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28803117/phenolics-from-rubus-fairholmianus-induces-cytotoxicity-and-apoptosis-in-human-breast-adenocarcinoma-cells
#17
Blassan P George, Heidi Abrahamse, Nanjundaswamy M Hemmaragala
Herbal medicine is an important part of health care system in most of the countries. Rubus fairholmianus is an unexplored berry in folkloric medicine. In this study, we aimed to understand the importance of R. fairholmianus in pharmaceutical industry for the development of cost-effective cancer therapeutic drugs using in vivo and in vitro analysis. Chemical characterisation, antioxidant, antiproliferative and apoptosis inducing properties of R. fairholmianus root methanolic column subfraction (RFM) were investigated...
August 9, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28802789/cytochrome-c-in-patients-undergoing-coronary-artery-bypass-grafting-a-post-hoc-analysis-of-a-randomized-trial
#18
Lars W Andersen, Xiaowen Liu, Sophia Montissol, Mathias J Holmberg, Bjørn K Fabian-Jessing, Michael W Donnino
PURPOSE: To establish whether plasma cytochrome c is detectable in patients undergoing cardiac surgery, whether cytochrome c levels are associated with lactate/inflammatory markers/cellular oxygen consumption, and whether cytochrome c levels are associated with clinical outcomes. MATERIALS AND METHODS: This was an observational sub-study of a randomized trial comparing thiamine to placebo in patients undergoing coronary artery bypass grafting. Patients had blood drawn before, after, and again 6h after surgery...
August 4, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/28802653/in-vitro-interactions-of-malachite-green-and-leucomalachite-green-with-hepatic-drug-metabolizing-enzyme-systems-in-the-rainbow-trout-onchorhyncus-mykiss
#19
Carlo Nebbia, Flavia Girolami, Monica Carletti, Laura Gasco, Ivo Zoccarato, Alessandra Giuliano Albo
Malachite green (MG) has been widely used in aquaculture to treat a number of microbial and parasitic diseases. It is currently banned in the EU because of the high cytotoxicity and carcinogenic activity, which is also shared by leucomalachite green (LMG), a reduced MG metabolite that can persist in fish tissues for months. There is scant information about the ability of either compound to interact with drug metabolizing enzymes in fish. Therefore we evaluated the in vitro effects of MG and LMG (25, 50 and 100μM) on some DMEs and glutathione (GSH) content in rainbow trout liver subfractions...
August 9, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28802176/a-rapid-and-simple-protocol-to-prepare-a-living-biocomposite-that-mimics-electroactive-biofilms
#20
Stéphane Pinck, Mathieu Etienne, Manuel Dossot, Frédéric P A Jorand
A living material was formed by self-assembly of bacterial cells (Shewanella oneidensis MR-1 or Pseudomonas fluorescens) with carbon nanotubes in the presence of cytochrome c from a bovine heart with the goal to mimic electroactive biofilms. The role of cytochrome c on self-assembly, cell viability and extracellular electron transfer was studied. Scanning electron microscopy and dynamic light scattering experiments highlighted its role on the self-assembly of bacteria‑carbon nanotube aggregates within only 2h in solution...
August 1, 2017: Bioelectrochemistry
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