keyword
MENU ▼
Read by QxMD icon Read
search

Cytochrome P

keyword
https://www.readbyqxmd.com/read/29458112/compatible-osmolytes-modulate-mitochondrial-function-in-a-marine-osmoconformer-crassostrea-gigas-thunberg-1793
#1
Eugene P Sokolov, Inna M Sokolova
Salinity is an important environmental factor affecting physiology of marine organisms. Osmoconformers such as marine mollusks maintain metabolic function despite changes of the osmolarity and composition of the cytosol during salinity shifts. Currently, metabolic responses to the salinity-induced changes of the intracellular milieu are not well understood. We studied the effects of osmolarity (450 vs. 900 mOsm) and compatible osmolytes (70-590 mM of taurine or betaine) on isolated gill mitochondria of a marine osmoconformer, the Pacific oyster Crassostrea gigas...
February 16, 2018: Mitochondrion
https://www.readbyqxmd.com/read/29456790/late-stage-microsomal-oxidation-reduces-drug-drug-interaction-and-identifies-phosphodiesterase-2a-inhibitor-pf-06815189
#2
Antonia F Stepan, Tuan P Tran, Christopher J Helal, Maria S Brown, Cheng Chang, Rebecca E O'Connor, Michael De Vivo, Shawn D Doran, Ethan L Fisher, Stephen Jenkinson, David Karanian, Bethany L Kormos, Raman Sharma, Gregory S Walker, Ann S Wright, Edward X Yang, Michael A Brodney, Travis T Wager, Patrick R Verhoest, R Scott Obach
Late-stage oxidation using liver microsomes was applied to phosphodiesterase 2 inhibitor 1 to reduce its clearance by cytochrome P450 enzymes, introduce renal clearance, and minimize the risk for victim drug-drug interactions. This approach yielded PF-06815189 ( 2 ) with improved physicochemical properties and a mixed metabolic profile. This example highlights the importance of C-H diversification methods to drug discovery.
February 8, 2018: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29454363/identification-of-wild-caught-phlebotomine-sand-flies-from-crete-and-cyprus-using-dna-barcoding
#3
Emmanouil Dokianakis, Nikolaos Tsirigotakis, Vasiliki Christodoulou, Nikos Poulakakis, Maria Antoniou
BACKGROUND: Phlebotomine sand flies (Diptera: Psychodidae) are vectors of Leishmania spp., protozoan parasites responsible for a group of neglected diseases called leishmaniases. Two sand fly genera, Phlebotomus and Sergentomyia, contain species that are present in the Mediterranean islands of Crete and Cyprus where the visceral (VL), cutaneous (CL) and canine (CanLei) leishmaniases are a public health concern. The risk of transmission of different Leishmania species can be studied in an area by monitoring their vectors...
February 17, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29453911/induction-of-xenobiotic-metabolizing-enzymes-in-hepatocytes-by-beta-naphthoflavone-time-dependent-changes-in-activities-protein-and-mrna-levels
#4
Kateřina Lněničková, Lenka Skálová, Lucie Stuchlíková, Barbora Szotáková, Petra Matoušková
In the present study, time-dependency of the induction effect of a selective inducer on the activity, protein and mRNA levels of cytochromes P450 1A1/2 (CYP1A1/2), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferases (GSTA), in primary culture of rat hepatocytes was tested and evaluated. To show the differences in responses of tested enzymes, the common aryl hydrocarbon receptor (AhR) ligand agonist, beta-naphthoflavone (BNF), was used. Induction of CYP1A1/2 by BNF was detected at all time intervals and at all levels (i...
March 1, 2018: Acta Pharmaceutica
https://www.readbyqxmd.com/read/29453687/impact-of-boosted-antiretroviral-therapy-on-the-pharmacokinetics-and-efficacy-of-clopidogrel-and-prasugrel-active-metabolites
#5
Niloufar Marsousi, Youssef Daali, Pierre Fontana, Jean-Luc Reny, Virginie Ancrenaz-Sirot, Alexandra Calmy, Serge Rudaz, Jules Alexandre Desmeules, Caroline Flora Samer
BACKGROUND AND OBJECTIVES: Prasugrel and clopidogrel are inhibitors of the ADP-P 2 Y 12 platelet receptor used in acute coronary syndrome patients. They require bioactivation via isoenzymes such as cytochrome P450 (CYP) 3A4, CYP2C19 and CYP2B6. Ritonavir and cobicistat are potent CYP3A inhibitors, prescribed as pharmacokinetic (PK) enhancers in the treatment of human immunodeficiency virus (HIV) infection. METHODS: In this study, the impact of boosted antiretroviral therapies (ARTs) on the PK of clopidogrel and prasugrel active metabolites (AMs), and on the efficacy of prasugrel and clopidogrel, were evaluated in a randomized crossover clinical trial...
February 16, 2018: Clinical Pharmacokinetics
https://www.readbyqxmd.com/read/29449808/suppressive-effects-of-clerodendrum-volubile-p-beauv-labiatae-methanolic-extract-and-its-fractions-on-type-2-diabetes-and-its-complications
#6
Ochuko L Erukainure, Rahman M Hafizur, Nurul Kabir, M Iqbal Choudhary, Olubunmi Atolani, Priyanka Banerjee, Robert Preissner, Chika I Chukwuma, Aliyu Muhammad, Eric O Amonsou, Md Shahidul Islam
Type 2 diabetes is the most prominent of all diabetes types, contributing to global morbidity and mortality. Availability and cost of treatment with little or no side effect especially in developing countries, remains a huge burden. This has led to the search of affordable alternative therapies especially from medicinal plants. In this study, the antidiabetic effect of the methanolic extract, dichloromethane (DCM), butanol (BuOH) and aqueous fractions of Clerodendrum volubile leaves were investigated in type 2 diabetic rats for their effect on glucose homeostasis, serum insulin level and hepatic biomarkers, lipid profile, pancreatic redox balance and Ca 2+ levels, and β-cell distribution and function...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29439571/characterized-in-vitro-metabolism-kinetics-of-alkyl-organophosphate-flame-retardants-in-fish-liver-and-intestinal-microsomes
#7
Rui Hou, Chao Huang, Kaifeng Rao, Yiping Xu, Zijian Wang
Tris(2-butoxyethyl) phosphate (TBOEP) and tris(n-butyl) phosphate (TNBP) are the most commonly used alkyl organophosphate esters (alkyl-OPEs), and they increasingly accumulate in organisms and create potential health hazards. This study examined the metabolism of TNBP and TBOEP in Carassius carassius liver and intestinal microsomes and the production of their corresponding mono-hydroxylated and dealkylated metabolites. After 140 min of incubation with fish liver microsomes, the rapid depletion of TNBP and TBOEP were both best fitted to Michaelis-Menten model (at administrated concentrations ranged from 0...
February 13, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29436156/cytochrome-p450-genetic-variation-associated-with-tamoxifen-biotransformation-in-american-indian-and-alaska-native-people
#8
Burhan A Khan, Renee Robinson, Alison E Fohner, LeeAnna I Muzquiz, Brian D Schilling, Julie A Beans, Matthew J Olnes, Laura Trawicki, Holly Frydenlund, Cindi Laukes, Patrick Beatty, Brian Phillips, Deborah Nickerson, Kevin Howlett, Denise A Dillard, Timothy A Thornton, Kenneth E Thummel, Erica L Woodahl
Despite evidence that pharmacogenetics can improve tamoxifen pharmacotherapy, there are few studies with American Indian and Alaska Native (AIAN) people. We examined variation in cytochrome P450 (CYP) genes (CYP2D6, CYP3A4, CYP3A5, and CYP2C9) and tamoxifen biotransformation in AIAN patients with breast cancer (n = 42) from the Southcentral Foundation in Alaska and the Confederated Salish and Kootenai Tribes in Montana. We tested for associations between CYP diplotypes and plasma concentrations of tamoxifen and metabolites...
February 13, 2018: Clinical and Translational Science
https://www.readbyqxmd.com/read/29434982/genipin-inhibits-the-growth-of-human-bladder-cancer-cells-via-inactivation-of-pi3k-akt-signaling
#9
Zheng Li, Tian-Biao Zhang, Dong-Hui Jia, Wen-Qi Sun, Chao-Liang Wang, Ao-Zheng Gu, Xiao-Ming Yang
Genipin, a natural compound derived from the fruit of Gardenia jasminoides, possesses numerous biological properties. The aim of the present study was to investigate the anticancer effects of genipin in human bladder cancer. T24 and 5637 bladder cancer cells were treated with different concentrations of genipin (0-200 µM) and tested for cell viability, colony formation, cell cycle progression and apoptosis. A xenograft model of bladder cancer was established to determine the anticancer effect of genipin in vivo...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434943/mechanism-of-anticancer-action-of-novel-berenil-complex-of-platinum-ii-combined-with-anti-muc1-in-mcf-7-breast-cancer-cells
#10
Agnieszka Gornowicz, Anna Bielawska, Wojciech Szymanowski, Halina Gabryel-Porowska, Robert Czarnomysy, Krzysztof Bielawski
Mucin 1 (MUC1) is a high molecular weight transmembrane glycoprotein, that is overexpressed in >90% of breast cancers. It serves a crucial role in anti-apoptosis and tumor progression. MUC1 interacts with proteins in the extracellular matrix, at the cell membrane, in the cytoplasm and in the nucleus. The aim of the present study was to investigate the mechanism of anticancer action induced by novel berenil complex of platinum(II) (Pt12) together with a monoclonal antibody against MUC1 in breast cancer MCF-7 cells...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434780/berbamine-induces-smmc-7721-cell-apoptosis-via-upregulating-p53-downregulating-survivin-expression-and-activating-mitochondria-signaling-pathway
#11
Ying Cao, Jianbo Cao, Binbin Yu, Shusheng Wang, Lili Liu, Li Tao, Wanping Sun
Hepatocellular carcinoma (HCC) is a primary malignancy in the liver, which is a global health problem. The present study aimed to observe the apoptotic effects of berbamine on SMMC-7721 cell lines and to investigate the effects of berbamine on induction of the intrinsic apoptotic pathway. The human HCC SMMC-7721 cells were cultured and cell morphology observed using a phase contrast microscope. SMMC-7721 cell apoptosis was examined by employing a flow cytometry assay. The nuclei of SMMC-7721 cells were stained with DAPI and observed by utilizing a laser fluorescence microscope...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29433569/mh84-improves-mitochondrial-dysfunction-in-a-mouse-model-of-early-alzheimer-s-disease
#12
Maximilian Pohland, Maren Pellowska, Heike Asseburg, Stephanie Hagl, Martina Reutzel, Aljoscha Joppe, Dirk Berressem, Schamim H Eckert, Mario Wurglics, Manfred Schubert-Zsilavecz, Gunter P Eckert
BACKGROUND: Current approved drugs for Alzheimer's disease (AD) only attenuate symptoms, but do not cure the disease. The pirinixic acid derivate MH84 has been characterized as a dual gamma-secretase/proliferator activated receptor gamma (PPARγ) modulator in vitro. Pharmacokinetic studies in mice showed that MH84 is bioavailable after oral administration and reaches the brain. We recently demonstrated that MH84 improved mitochondrial dysfunction in a cellular model of AD. In the present study, we extended the pharmacological characterization of MH84 to 3-month-old Thy-1 AβPPSL mice (harboring the Swedish and London mutation in human amyloid precursor protein (APP)) which are characterized by enhanced AβPP processing and cerebral mitochondrial dysfunction, representing a mouse model of early AD...
February 13, 2018: Alzheimer's Research & Therapy
https://www.readbyqxmd.com/read/29432897/bleeding-predictors-in-patients-following-venous-thromboembolism-treated-with-vitamin-k-antagonists-association-with-increased-number-of-single-nucleotide-polymorphisms
#13
Agata Hanna Bryk, Ewa Wypasek, Krzysztof Plens, Magdalena Awsiuk, Anetta Undas
INTRODUCTION: Single nucleotide polymorphisms (SNP) in genes encoding proteins involved in metabolism and action of vitamin K antagonists (VKA) affect anticoagulation stability. We investigated how those polymorphisms influence bleeding rates in patients following venous thromboembolism (VTE). MATERIALS AND METHODS: In 324 patients following unprovoked VTE, 143 (44%) on warfarin and 181 (56%) on acenocoumarol, we recorded bleedings within the preceding 24 months...
February 9, 2018: Vascular Pharmacology
https://www.readbyqxmd.com/read/29432141/structure-and-function-of-the-cytochrome-p450-peroxygenase-enzymes
#14
REVIEW
Andrew W Munro, Kirsty J McLean, Job L Grant, Thomas M Makris
The cytochromes P450 (P450s or CYPs) constitute a large heme enzyme superfamily, members of which catalyze the oxidative transformation of a wide range of organic substrates, and whose functions are crucial to xenobiotic metabolism and steroid transformation in humans and other organisms. The P450 peroxygenases are a subgroup of the P450s that have evolved in microbes to catalyze the oxidative metabolism of fatty acids, using hydrogen peroxide as an oxidant rather than NAD(P)H-driven redox partner systems typical of the vast majority of other characterized P450 enzymes...
February 6, 2018: Biochemical Society Transactions
https://www.readbyqxmd.com/read/29431222/saurogobio-punctatus-sp-nov-a-new-cyprinid-gudgeon-teleostei-cypriniformes-from-the-yangtze-river-based-on-both-morphological-and-molecular-data
#15
Q Y Tang, X B Li, D Yu, Y R Zhu, B Q Ding, H Z Liu, P D Danley
A new cyprinid gudgeon, Saurogobio punctatus sp. nov., is described based on specimens collected from the Yangtze River, China. The new species can be distinguished from its congeners by differences in both morphology and the cytochrome b (cytb) gene sequence. Numerous minute blackish spots are scattered on dorsal and caudal fins in S. punctatus sp. nov. v. absent in the other seven valid Saurogobio species. The new species can be further distinguished from its congeners by the following unique combination of characters: a dorsal fin with eight branched rays; absence of scales in chest area before pectoral origin; upper and lower lips thick, covered with papillae; and a papillose mental pad approximately triangular...
February 2018: Journal of Fish Biology
https://www.readbyqxmd.com/read/29426916/2-methoxyestradiol-protects-against-pressure-overload-induced-left-ventricular-hypertrophy
#16
Zaid H Maayah, Jody Levasseur, Ramanaguru Siva Piragasam, Ghada Abdelhamid, Jason R B Dyck, Richard P Fahlman, Arno G Siraki, Ayman O S El-Kadi
Numerous experimental studies have supported the evidence that 2-methoxyestradiol (2 ME) is a biologically active metabolite that mediates multiple effects on the cardiovascular system, largely independent of the estrogen receptor. 2 ME is a major cytochrome P450 1B1 (CYP1B1) metabolite and has been reported to have vasoprotective and anti-inflammatory actions. However, whether 2 ME would prevent cardiac hypertrophy induced by abdominal aortic constriction (AAC) has not been investigated yet. Therefore, the overall objectives of the present study were to elucidate the potential antihypertrophic effect of 2 ME and explore the mechanism(s) involved...
February 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29426278/cytochrome-p450-family-4-subfamily-f-member-2-cyp4f2-rs1558139-rs2108622-polymorphisms-and-susceptibility-to-several-cardiovascular-and-cerebrovascular-diseases
#17
Tao Zhang, Kuiying Yu, Xuhua Li
BACKGROUND: Inconsistent conclusions have been reported for the genetic relationship between CYP4F2 (Cytochrome P450 Family 4 Subfamily F Member 2) polymorphisms and the susceptibility to cardiovascular and cerebrovascular diseases. METHODS: We performed a meta-analysis to assess the potential role of rs1558139 C/T and rs2108622 G/A polymorphisms of CYP4F2 in the risks of cardiovascular and cerebrovascular diseases. The retrieval of four databases, including PubMed, Web of Science (WOS), China National Knowledge Infrastructure (CNKI) and WANFANG DATA, was conducted...
February 9, 2018: BMC Cardiovascular Disorders
https://www.readbyqxmd.com/read/29423652/deep-sequencing-of-the-mitochondrial-genome-reveals-common-heteroplasmic-sites-in-nadh-dehydrogenase-genes
#18
Chunyu Liu, Jessica L Fetterman, Poching Liu, Yan Luo, Martin G Larson, Ramachandran S Vasan, Jun Zhu, Daniel Levy
Increasing evidence implicates mitochondrial dysfunction in aging and age-related conditions. But little is known about the molecular basis for this connection. A possible cause may be mutations in the mitochondrial DNA (mtDNA), which are often heteroplasmic-the joint presence of different alleles at a single locus in the same individual. However, the involvement of mtDNA heteroplasmy in aging and age-related conditions has not been investigated thoroughly. We deep-sequenced the complete mtDNA genomes of 356 Framingham Heart Study participants (52% women, mean age 43, mean coverage 4570-fold), identified 2880 unique mutations and comprehensively annotated them by MITOMAP and PolyPhen-2...
February 8, 2018: Human Genetics
https://www.readbyqxmd.com/read/29423002/ndufa4-enhances-neuron-growth-by-triggering-growth-factors-and-inhibiting-neuron-apoptosis-through-bcl-2-and-cytochrome-c-mediated-signaling-pathway
#19
Fang Fu, Yan Li, Ru Li, Ting-Ying Lei, Dan Wang, Xin Yang, Jin Han, Min Pan, Li Zhen, Yan-Mei Ou, Jian Li, Fao-Tao Li, Xiang-Yi Jing, Dong-Zhi Li, Can Liao
Dandy-Walker malformation (DWM) is the most prevalent congenital malformation in cerebellum, however, pathological mechanism of DWM has not been fully clarified. This study aims to investigate effects of NDUFA4 on growth of neurons. LV5-NDUFA4 and LV3-NDUFA4-RNAi lentivirus were constructed and transfected to neurons. Ciclosporin A, together with the two lentivirus were applied to neurons to observe neuron growth, apoptosis, and related protein expression. MTT assay was used to observe neuron growth. Apoptosis was detected by using flow cytometry assay...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/29416685/estrogen-er%C3%AE-signaling-axis-participates-in-osteoblast-maturation-via-upregulating-chromosomal-and-mitochondrial-complex-gene-expressions
#20
Pei-I Lin, Yu-Ting Tai, Wing P Chan, Yi-Ling Lin, Mei-Hsiu Liao, Ruei-Ming Chen
Estrogen deficiency usually leads to bone loss and osteoporosis in postmenopausal women. Osteoblasts play crucial roles in bone formation. However, osteoblast functions are influenced by mitochondrial bioenergetic conditions. In this study, we investigated the roles of the estrogen and estrogen receptor alpha (ERα) axis in mitochondrial energy metabolism and subsequent osteoblast mineralization. Exposure of rat calvarial osteoblasts to estradiol caused substantial improvements in alkaline phosphatase activities and cell calcification...
January 2, 2018: Oncotarget
keyword
keyword
51903
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"