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https://www.readbyqxmd.com/read/28738615/electrochemical-magneto-immunosensor-based-on-endogenous-%C3%AE-galactosidase-enzyme-to-determine-enterotoxicogenic-escherichia-coli-f4-k88-in-swine-feces-using-square-wave-voltammetry
#1
Lorena Viviana Tarditto, María Alicia Zon, Hugo García Ovando, Nelio Roberto Vettorazzi, Fernando Javier Arévalo, Héctor Fernández
Diseases caused by enterotoxicogenic Escherichia coli F4 (K88) (ETEC F4) are a problem in swine production establishments. Due to the high rate of mortality and morbidity of E. coli infections, a rapid and accurate diagnosis is important in order to choose an appropriate treatment to reduce the economic impact. Therefore, an electrochemical magneto-immunosensor (EMI) was developed to detect and quantify ETEC F4 in swine feces samples through a direct non-competitive immunoassay. ETEC F4 was selectively captured by immunomagnetic separation...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738471/-association-between-phage-mediated-shiga-toxin-and-molecular-distribution-of-crispr-in-escherichia-coli-o26-%C3%A2-h11-or-nm
#2
J Z Long, Y K Xu, G C Duan, W J Liang, H Y Liu, S Y Chen, Y L Xi, P F Wang, Y F Wang
Objective: To investigate the association between phage-mediated shiga toxin and molecular distribution of CRISPR in Escherichia (E.) coli O26∶H11 or NM. Methods: A total of 135 E. coli O26 ∶ H11 or NM strains were collected from NCBI database. Software CRT and CRISPR Finder were used to extract CRISPR and Excel was used to assign the spacer of unique number and type CRISPR. And the relationship between CRISPR and stx phage was analyzed. Results: All the 135 E. coli O26 ∶ H11 or NM strains had the CRISPR...
July 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28738194/characterization-and-horizontal-transfer-of-qach-associated-class-1-integrons-in-escherichia-coli-isolated-from-retail-meats
#3
Xiaobing Jiang, Yameng Xu, Yi Li, Kun Zhang, Lei Liu, Hailei Wang, Jinhe Tian, Hao Ying, Lei Shi, Tao Yu
The aim of this study was to examine the presence of genes responsible for resistance to quaternary ammonium compounds (QACs) and the association of qac genes with class 1 integrons in Escherichia coli isolated from retail meats. Among the 179 E. coli isolates tested, the minimum inhibitory concentrations (MICs) of benzalkonium chloride (BC) ranged from 4 to 64μg/mL. PCR assays indicated that QAC-resistance genes sugE(c), ydgE/ydgF, mdfA, emrE and qacEΔ1 were commonly present (40.2%-88.3%) in these isolates, but qacE, qacF, qacH and sugE(p) were less prevalent (2...
July 15, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28737940/defining-the-structural-basis-for-allosteric-product-release-from-e-coli-dihydrofolate-reductase-using-nmr-relaxation-dispersion
#4
David Oyen, R Bryn Fenwick, Phillip C Aoto, Robyn L Stanfield, Ian A Wilson, H Jane Dyson, Peter E Wright
The rate-determining step in the catalytic cycle of E.coli dihydrofolate reductase is tetrahydrofolate (THF) product release, which can occur via an allosteric or an intrinsic pathway. The allosteric pathway, which becomes accessible when the reduced cofactor NADPH is bound, involves transient sampling of a higher energy conformational state, greatly increasing the product dissociation rate compared to the intrinsic pathway that obtains when NADPH is absent. Although the kinetics of this process are known, the enzyme structure and the THF product conformation in the transiently formed excited state remains elusive...
July 24, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28737752/mechanical-strain-sensing-implicated-in-cell-shape-recovery-in-escherichia-coli
#5
Felix Wong, Lars D Renner, Gizem Özbaykal, Jayson Paulose, Douglas B Weibel, Sven van Teeffelen, Ariel Amir
The shapes of most bacteria are imparted by the structures of their peptidoglycan cell walls, which are determined by many dynamic processes that can be described on various length scales ranging from short-range glycan insertions to cellular-scale elasticity(1-11). Understanding the mechanisms that maintain stable, rod-like morphologies in certain bacteria has proved to be challenging due to an incomplete understanding of the feedback between growth and the elastic and geometric properties of the cell wall(3,4,12-14)...
July 24, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28736954/the-impact-of-the-bovine-fecal-microbiome-on-escherichia-coli-o157-h7-prevalence-and-enumeration-in-naturally-infected-cattle
#6
M Kim, L A Kuehn, J L Bono, E D Berry, N Kalchayanand, H C Freetly, A K Benson, J E Wells
AIMS: The objective of this study was to determine if the fecal microbiome has an association with E. coli O157:H7 prevalence and enumeration. METHODS AND RESULTS: Pyrosequencing analysis of fecal microbiome was performed from feedlot cattle fed one of three diets: i) 94 heifers fed low concentrate (LC) diet, ii) 142 steers fed moderate concentrate (MC) diet, and iii) 132 steers fed high concentrate (HC) diet. A total of 322,585 OTUs were calculated from 2,411,122 high-quality sequences obtained from 368 fecal samples...
July 23, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28736752/inhibitory-activity-of-a-concanavalin-isolated-fraction-from-a-glucosamine-peptides-reaction-system-against-heat-resistant-e-coli
#7
Yuliya Hrynets, Daylin Johana Hincapie Martinez, Maurice Ndagijimana, Mirko Betti
Alcalase-derived gelatin hydrolysates were glycated with glucosamine in the presence (+) or absence (-) of transglutaminase (TGase), and their antimicrobial activities toward Escherichia coli AW 1.7 were studied. Glycation treatments were subjected to concanavalin A affinity chromatography to selectively collect the glycopeptide-enriched fractions and the changes in antimicrobial activity were determined. The minimum inhibitory concentration of glycated hydrolysates decreased by 1.2 times compared to the native hydrolysate, with no differences between (+) or (-) TGase treatments...
July 2017: Heliyon
https://www.readbyqxmd.com/read/28736549/cloning-expression-isotope-labeling-and-purification-of-transmembrane-protein-merf-from-mercury-resistant-enterobacter-sp-az-15-for-nmr-studies
#8
Aatif Amin, Zakia Latif
Mercury resistant (Hg(R)) Enterobacter sp. AZ-15 was isolated from heavy metal polluted industrial wastewater samples near to districts Kasur and Sheikhupura, Pakistan. 16S rDNA ribotyping and phylogentic analysis showed 98% homology with already reported Enterobacter species. The merF gene encoding transmembrane protein-MerF was amplified from genomic DNA and ligated into pET31b+ vector using restriction endonucleases, SphI and XhoI. The genetic codons of merF gene encoding cysteine residues were mutated into codons, translating into serine residues by site-directed mutagenesis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28736197/characterization-of-an-escherichia-coli-derived-human-papillomavirus-type-16-and-18-bivalent-vaccine
#9
Ying Gu, Minxi Wei, Daning Wang, Zhihai Li, Minghui Xie, Huirong Pan, Ting Wu, Jun Zhang, Shaowei Li, Ningshao Xia
Human papillomavirus (HPV) types 16 and 18 account for approximately 70% of cervical cancer worldwide. Neutralizing HPV prophylactic vaccines offer significant benefit, as they block HPV infection and prevent subsequent disease. However, the three licensed HPV vaccines that cover these two genotypes were produced in eukaryotic cells, which is expensive, particularly for low-income countries where HPV is highest. Here, we report a new HPV16 and -18 bivalent candidate vaccine produced from Escherichia coli. We used two strategies of N-terminal truncation of HPV L1 proteins and soluble non-fusion expression to generate HPV16 and HPV18 L1-only virus-like particles (VLPs) in a scalable process...
July 20, 2017: Vaccine
https://www.readbyqxmd.com/read/28736041/construction-of-multilayer-alginate-hydrogel-beads-for-oral-delivery-of-probiotics-cells
#10
Yang Li, Chao Feng, Jing Li, Yuzhi Mu, Ya Liu, Ming Kong, Xiaojie Cheng, Xiguang Chen
Multilayer alginate hydrogel beads (MAHBs), which were prepared by emulsion method, had been fabricated via the ionic crosslinking between calcium ion (Ca(2+)) and carboxylic group of alginate and utilized as encapsulating material for oral delivery of a model probiotic bacteria Bifidobacterium breve. Optical and fluorescence microscopy obviously showed the microorganism cells were encapsulated in the core beads. The viability of B. breve cells encapsulated in MAHBs was significantly enhanced compared with ordinary free cultured ones, the top CFU per mL were (3...
July 20, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28735356/evaluation-of-anthelmintic-antimicrobial-and-antioxidant-activity-of-chenopodium-album
#11
Bashir A Lone, M Z Chishti, Fayaz Ahmad Bhat, Hidayatullah Tak, Suhaib A Bandh, Abida Khan
The objective of this study was to evaluate the anthelmintic, antimicrobial and antioxidant activities of Chenopodium album against gastrointestinal nematodes of sheep and some pathogenic microbes. A worm motility inhibition assay was used for in vitro study, and a faecal egg count reduction assay was used for an in vivo study. Various concentrations ranging from 100 to 500 μg/ml of the extract were subjected to antimicrobial screening by disc diffusion method against four selected bacterial (Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas multocida and Escherichia coli) and two fungal (Aspergillus flavus and Candida albicans) strains in order to estimate the medicinal potential of the herb...
July 22, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28735241/a-new-integrated-approach-to-analyze-bulk-tank-milk-and-raw-milk-filters-for-the-presence-of-the-e-coli-serogroups-frequently-associated-with-vtec-status
#12
Francesca Albonico, Claudia Gusmara, Theo Gugliotta, Monica Loiacono, Michele Mortarino, Alfonso Zecconi
We optimized a combination of microbiological and molecular methods to quickly identify the presence of the O157 and the six non-O157 serogroups (O26, O45, O103, O111, O121 and O145) most frequently associated with VTEC status, at herd level. The lower detection limit of this methodology is 10(1)CFU/ml for each of the serogroups tested. We tested 67 bulk tank milk (BTM) and raw milk filters (RMF) derived from dairy herds located in Lombardy and Trentino Alto Adige. We identified 3 positive samples and 20 positive samples out of 67 respectively in the BTM and RMF...
July 17, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28735058/prevalence-of-pmqr-genes-in-e-coli-and-klebsiella-spp-isolated-from-north-west-of-morocco
#13
Houda Benaicha, Said Barrijal, Fatima Ezzakkioui, Fatima Elmalki
OBJECTIVES: Plasmid mediated quinolone resistance (PMQR) genes are increasingly detected worldwide. However, in Morocco a few studies have been carried out. The aim of this study was to investigate the prevalence of these determinants among Escherichia coli and Klebsiella spp isolated from North-West of Morocco. METHODS: The prevalence of PMQR genes was investigated among 398 E. coli and 118 Klebsiella spp clinical isolates collected between 2012 and 2015 from North-West of Morocco...
July 19, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28735057/brazil-s-resolutions-to-regulate-the-sale-of-antibiotics-impact-on-consumption-and-escherichia-coli-resistance-rates
#14
Karen P H Mattos, Marília B Visacri, Júlia C F Quintanilha, Gustavo R Lloret, Maria A Cursino, Anna S Levin, Carlos E Levy, Patricia Moriel
OBJECTIVE: The aim of this study was to determine the impact of two resolutions to restrict antibiotic use (RDCs no. 44/2010 and 20/2011) in the Campinas metropolitan area (Sao Paulo, Brazil) on antibiotic consumption, resistance rates, and trends in Escherichia coli-causing community-acquired urinary tract infection (UTI). METHODS: The annual retail sale information of antibiotics from drugstores in the Campinas metropolitan area between 2008 and 2012 were obtained through the Intercontinental Medical Statistics Health of Brazil...
July 19, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28734978/insights-into-the-functional-properties-of-the-marneral-oxidase-cyp71a16-from-arabidopsis-thaliana
#15
Sarah Kranz-Finger, Osama Mahmoud, Esther Ricklefs, Nikolas Ditz, Patrick J Bakkes, Vlada B Urlacher
The Arabidopsis thaliana gene encoding CYP71A16 is part of the gene cluster for the biosynthesis and modification of the triterpenoid marneral. Previous investigations of A. thaliana have revealed that CYP71A16 oxidizes marneral, while it also can accept marnerol as substrate. The aim of the present study was to investigate functional properties of CYP71A16 in vitro. For this purpose, heterologous expression of a N-terminally modified version of CYP71A16 was established in Escherichia coli, which yielded up to 50mg·L(-1) recombinant enzyme...
July 19, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28734896/porcine-cd14-gene-silencing-partially-inhibited-the-bacterial-immune-response-mediated-by-tlr4-signaling-pathway
#16
Chaohui Dai, Haifei Wang, Guoqiang Zhu, Shenglong Wu, Wenbin Bao
Cluster of differentiation antigen 14 (CD14) is the membrane receptor protein in Toll-like Receptor 4 (TLR4) signaling pathway, which plays an important regulation role in not only innate immune response but also adaptive immune response. In this study, the pig kidney epithelial cell (PK15) line with CD14 gene silencing mediated by lentivirus was established and cells of CD14-RNAi and NC group were exposed to three kinds of Escherichia coli (E. coli F18ab, E. coli F18ac and E. coli K88ac) and LPS. Then qPCR and western blot were used to detect expression levels of TLR4 signaling pathway-related genes...
July 19, 2017: Gene
https://www.readbyqxmd.com/read/28734838/expression-purification-and-characterization-of-sll1981-protein-from-cyanobacterium-synechocystis-sp-pcc6803
#17
Xiaoqin Wang, Guofeng Lei, Xiaoyu Wu, Fei Wang, Chongde Lai, Zhimin Li
The sll1981 protein from cyanobacterium Synechocystis sp. PCC6803 had been reported to exhibit acetolactate synthase (ALS) and L-myo-inositol-1-phosphate synthase (MIPS) activities previously. Based on amino acids sequences alignment, sll1981 protein was postulated to function as α-ketoglutarate decarboxylase (α-KGD), which played important role in completing cyanobacterial tricarboxylic acid (TCA) cycle. However the detailed enzymatic kinetics of sll1981 as ALS, MIPS and α-KGD were not determined yet. In this study, the recombinant sll1981 protein was purified from supernatant of E...
July 19, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28734365/automatic-feed-phase-identification-in-multivariate-bioprocess-profiles-by-sequential-binary-classification
#18
Ramin Nikzad-Langerodi, Edwin Lughofer, Susanne Saminger-Platz, Thomas Zahel, Patrick Sagmeister, Christoph Herwig
In this paper, we propose a new strategy for retrospective identification of feed phases from online sensor-data enriched feed profiles of an Escherichia Coli (E. coli) fed-batch fermentation process. In contrast to conventional (static), data-driven multi-class machine learning (ML), we exploit process knowledge in order to constrain our classification system yielding more parsimonious models compared to static ML approaches. In particular, we enforce unidirectionality on a set of binary, multivariate classifiers trained to discriminate between adjacent feed phases by linking the classifiers through a one-way switch...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28733784/environmental-impact-of-coal-mining-and-coal-seam-gas-production-on-surface-water-quality-in-the-sydney-basin-australia
#19
A Ali, V Strezov, P Davies, I Wright
The extraction of coal and coal seam gas (CSG) will generate produced water that, if not adequately treated, will pollute surface and groundwater systems. In Australia, the discharge of produced water from coal mining and related activities is regulated by the state environment agency through a pollution licence. This licence sets the discharge limits for a range of analytes to protect the environment into which the produced water is discharged. This study reports on the impact of produced water from coal mine activities located within or discharging into high conservation environments, such as National Parks, in the outer region of Sydney, Australia...
August 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28733289/an-enterotoxin-like-binary-protein-from-pseudomonas-protegens-with-potent-nematicidal-activity
#20
Jun-Zhi Wei, Daniel L Siehl, Zhenglin Hou, Barbara Rosen, Jarred Oral, Christopher G Taylor, Gusui Wu
Soil microbes are a major food source for free-living soil nematodes. It is known that certain soil bacteria have evolved systems to combat predation. We identified the nematode-antagonistic Pseudomonas protegens strain 15G2 from screening of microbes. Through protein purification we identified a binary protein, designated as Pp-ANP, that is responsible for the nematicidal activity. This binary protein inhibits Caenorhabditis elegans growth and development by arresting larvae at L1 stage and killing older staged worms...
July 21, 2017: Applied and Environmental Microbiology
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