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https://www.readbyqxmd.com/read/28441664/protection-against-legionnaire-s-disease-recombinant-flagellin-a-of-legionella-pneumophila-can-induce-protective-immunity-against-bacteremia-in-a-balb-c-murine-model
#1
Ashraf Mohabati Mobarez, Roya Ahmadrajabi, Nima Khoramabadi, Ali-Hatef Salmanian
To investigate the immunoprotective effects of the recombinant type A flagellin (FLA), the flaA gene of Legionella pneumophila serogroup 1 strain Paris was cloned into pET28a(+). Recombinant FLA (rFLA) was overexpressed in E. coli BL21 (DE3) and purified by Ni2+ exchange chromatography. Female BALB/c aged 6-8 weeks were immunized with 20 μg of rFLA. Nonimmunized mice along with mice inoculated with a sublethal dose of live L. pneumophila intravenously were considered as negative and positive controls, respectively...
April 26, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28441472/repurposing-a-two-component-system-based-biosensor-for-the-killing-of-vibrio-cholerae
#2
Premkumar Jayaraman, Maciej Bartosz Holowko, Jing Wui Yeoh, Sierin Lim, Chueh Loo Poh
New strategies to control cholera are urgently needed. This study develops an in vitro proof-of-concept sense-and-eradicate system in a wild-type Escherichia coli strain to target the causative pathogen Vibrio cholerae using a synthetic biology approach. Our engineered E. coli specifically detects V. cholerae via its quorum-sensing molecule CAI-1 and responds by expressing the lysis protein (YebF-Art-085), thereby self-lysing to release the killing protein (Art-085) to eradicate V. cholerae. For this report, we individually characterized the YebF-Art-085 and Art-085 expression and their activities when coupled to our previously developed V...
April 25, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28441427/programmable-type-iii-a-crispr-cas-dna-targeting-modules
#3
H Travis Ichikawa, John C Cooper, Leja Lo, Jason Potter, Rebecca M Terns, Michael P Terns
The CRISPR-Cas systems provide invader defense in a wide variety of prokaryotes, as well as technologies for many powerful applications. The Type III-A or Csm CRISPR-Cas system is one of the most widely distributed across prokaryotic phyla, and cleaves targeted DNA and RNA molecules. In this work, we have constructed modules of Csm systems from 3 bacterial species and heterologously expressed the functional modules in E. coli. The modules include a Cas6 protein and a CRISPR locus for crRNA production, and Csm effector complex proteins...
2017: PloS One
https://www.readbyqxmd.com/read/28441350/synthesis-and-bioactivity-evaluation-of-novel-2-salicyloylbenzofurans-as-antibacterial-agents
#4
Phuong-Thuy T Phan, Thu-Trang T Nguyen, Hong-Nhung T Nguyen, Bao-Khanh N Le, Thao T Vu, Dong C Tran, Tuan-Anh N Pham
In order to discover new antibacterial agents, series of 2-salicyloylbenzofuran derivatives were designed, synthesized and evaluated for their antibacterial activities against three Gram-(+) strains (methicillin-sensitive Staphylococcus aureus (MSSA) ATCC 29213, methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300, and Streptococcus faecalis (S. faecalis) ATCC 29212) and one Gram-(-) strain (Escherichia coli (E. coli) ATCC 25922). The 2-salicyloylbenzofuran heterocycles were generated by Rap-Stoermer condensation of salicylaldehydes with phenacyl bromides and then converted to diverse O-ether derivatives by Williamson synthesis...
April 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28441310/a-dose-response-curve-for-a-gram-negative-spinal-implant-infection-model-in-rabbits
#5
Joseph L Laratta, Jamal N Shillingford, Nathan Hardy, Ronald A Lehman, Lawrence G Lenke, K Daniel Riew
STUDY DESIGN: Randomized complete block animal spinal implant infection model with internal control. OBJECTIVE: To develop a spinal implant animal infection model to simulate postoperative gram-negative wound infection. SUMMARY OF BACKGROUND DATA: Implant-associated surgical site infections (SSI) remain a dreaded complication of spinal surgery. Currently, over 30% of all spine SSIs are secondary to gram-negative bacteria. Traditional animal models have utilized gram-positive inoculums to simulate postoperative infection, but there exists no model in the literature for gram-negative infection in the setting of spinal instrumentation...
April 24, 2017: Spine
https://www.readbyqxmd.com/read/28440667/camelus-dromedarius-glucose-transporter-4-in-silico-analysis-cloning-expression-purification-and-characterisation-in-e-coli
#6
Anwar Ahmed, Mohammed Arshad, Ajamaluddin Malik, Shama Parveen, Abdulrahman M Alsenaidy
Camels have exceptional carbohydrate metabolism as their plasma glucose level is high and have low whole body insulin sensitivity, similar to that observed in type 2 diabetes patients. We aimed at studing an important component of insulin signalling pathway, the GLUT4, in camel. Camelus dromedarius GLUT4 (CdGLUT4) CDS is 1530 nucleotide in length that encodes for a 55KDa protein. CdGLUT4 has 23 amino acid substitutions and 3N-glycosylation sites, compared to 2 in Human GLUT4. 3 D structures of CdGLUT4 and HsGLUT4 generated by homology modelling revealed conservation of characteristic signature motifs...
April 25, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28440618/design-of-antibacterial-polyethersulfone-membranes-via-covalently-attaching-hydrogel-thin-layers-loaded-with-ag-nanoparticles
#7
Min He, Qian Wang, Rui Wang, Yi Xie, Weifeng Zhao, Changsheng Zhao
In order to inhibit the bacteria attachment and the subsequent formation of bio-films on polyethersulfone (PES) membranes, poly(sulfobetaine methacrylate) (PSBMA)/poly(sodium acrylate) (PAANa) antibacterial hydrogel thin layers were covalently attached onto the membranes, followed by loading Ag nanoparticles. In our strategy, double bonds were firstly introduced onto PES membrane surfaces to provide anchoring sites, and then the hydrogel layers were synthesized on the membrane surfaces via UV light initiated cross-linking copolymerization...
April 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28440329/role-of-the-virulence-plasmid-in-acid-resistance-of-shigella-flexneri
#8
Chang Niu, Jing Yang, Hongsheng Liu, Yong Cui, Huijie Xu, Ruifeng Wang, Xiankai Liu, Erling Feng, Dongshu Wang, Chao Pan, Wei Xiao, Xiaoqing Liu, Li Zhu, Hengliang Wang
Virulence plasmid (VP) acquisition was a key step in the evolution of Shigella from a non-pathogenic Escherichia coli ancestor to a pathogenic genus. In addition, the co-evolution and co-ordination of chromosomes and VPs was also a very important step in the evolutionary process. To investigate the cross-talk between VPs and bacterial chromosomes, we analyzed the expression profiles of protein complexes and protein monomers in three wild-type Shigella flexneri strains and their corresponding VP deletion mutants...
April 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28439785/influence-of-environmental-conditions-on-norovirus-presence-in-mussels-harvested-in-montenegro
#9
Nevena Ilic, Branko Velebit, Vlado Teodorovic, Vesna Djordjevic, Nedjeljko Karabasil, Dragan Vasilev, Spomenka Djuric, Bojan Adzic, Mirjana Dimitrijevic
This study comprises the first systematic survey of the occurrence of Norovirus in Mediterranean mussels from harvesting areas in Montenegro coast of Adriatic Sea. Mussels may accumulate contaminants of public health concern, including pathogenic bacteria and viruses. Microbiological monitoring of harvesting areas is based on count of Escherichia coli in bivalve molluscs in the European Union. It is assumed that E. coli does not reflect contamination with enteric viruses. A structured field study was undertaken at six locations in Bay of Kotor, Montenegro, in order to investigate plausible influence of environmental factors on the variability of E...
April 24, 2017: Food and Environmental Virology
https://www.readbyqxmd.com/read/28439602/antibiogram-profile-of-septic-meningitis-among-children-in-duhok-iraq
#10
Abdulrahman T Saadi, Nadir A Garjees, Aveen H Rasool
  To determine the prevalence of bacteria in the cerebrospinal fluid (CSF), and the antibiogram profile in pediatric patients with suspected meningitis. Methods: This descriptive study was conducted between January 2014 and January 2016 in the Hevi Paediatric Teaching Hospital in Duhok, Iraq. The CSF samples were withdrawn from 432 pediatric patients suspected of meningitis. The samples were cultured, and antibiotic sensitivity tests were performed.  Results: There were 33 (7.6%) culture positive cases among 432 CSF samples...
May 2017: Saudi Medical Journal
https://www.readbyqxmd.com/read/28439298/multi-drug-resistant-pathogenic-bacteria-in-the-gut-of-young-children-in-bangladesh
#11
Shirajum Monira, Syeda Antara Shabnam, Sk Imran Ali, Abdus Sadique, Fatema-Tuz Johura, Kazi Zillur Rahman, Nur Haque Alam, Haruo Watanabe, Munirul Alam
BACKGROUND: The gut of human harbors diverse commensal microbiota performing an array of beneficial role for the hosts. In the present study, the major commensal gut bacteria isolated by culturing methods from 15 children of moderate income families, aged between 10 and 24 months, were studied for their response to different antibiotics, and the molecular basis of drug resistance. RESULTS: Of 122 bacterial colonies primarily selected from Luria-Bertani agar, bacterial genera confirmed by analytical profile index (API) 20E(®) system included Escherichia as the predominant (52%) organism, followed by Enterobacter (16%), Pseudomonas (12%), Klebsiella (6%), Pantoea (6%), Vibrio (3%), and Citrobacter (3%); while Aeromonas and Raoultella were identified as the infrequently occurring genera...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28439280/insights-on-structure-and-function-of-a-late-embryogenesis-abundant-protein-from-amaranthus-cruentus-an-intrinsically-disordered-protein-involved-in-protection-against-desiccation-oxidant-conditions-and-osmotic-stress
#12
Alma L Saucedo, Eric E Hernández-Domínguez, Luis A de Luna-Valdez, Angel A Guevara-García, Abraham Escobedo-Moratilla, Esaú Bojorquéz-Velázquez, Federico Del Río-Portilla, Daniel A Fernández-Velasco, Ana P Barba de la Rosa
Late embryogenesis abundant (LEA) proteins are part of a large protein family that protect other proteins from aggregation due to desiccation or osmotic stresses. Recently, the Amaranthus cruentus seed proteome was characterized by 2D-PAGE and one highly accumulated protein spot was identified as a LEA protein and was named AcLEA. In this work, AcLEA cDNA was cloned into an expression vector and the recombinant protein was purified and characterized. AcLEA encodes a 172 amino acid polypeptide with a predicted molecular mass of 18...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28439262/turn-up-the-heat-food-and-clinical-escherichia-coli-isolates-feature-two-transferrable-loci-of-heat-resistance
#13
Erik J Boll, Roger Marti, Henrik Hasman, Søren Overballe-Petersen, Marc Stegger, Kim Ng, Susanne Knøchel, Karen A Krogfelt, Joerg Hummerjohann, Carsten Struve
Heat treatment is a widely used process to reduce bacterial loads in the food industry or to decontaminate surfaces, e.g., in hospital settings. However, there are situations where lower temperatures must be employed, for instance in case of food production such as raw milk cheese or for decontamination of medical devices such as thermo-labile flexible endoscopes. A recently identified locus of heat resistance (LHR) has been shown to be present in and confer heat resistance to a variety of Enterobacteriaceae, including Escherichia coli isolates from food production settings and clinical ESBL-producing E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28439124/characterisation-of-a-cell-free-synthesised-g-protein-coupled-receptor
#14
Patrick J Shilling, Fabian Bumbak, Daniel J Scott, Ross A D Bathgate, Paul R Gooley
G-protein coupled receptors are the largest family of integral membrane proteins found within the human genome. They function as receptors and modulators to a wide range of ligands and responses which are crucial for human health. GPCR study, specifically the investigation of structure and interaction to cognate ligands, is of high priority. Limitations for structural study can be traced in part, to obtaining suitable quantities of recombinant protein. We sought to address the limitations of traditional recombinant technologies by utilising an Escherichia coli based cell-free protein synthesis (CFPS) approach for production of a thermostable neurotensin receptor 1 (en2NTS1)...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28439041/cues-from-the-membrane-bacterial-glycerophospholipids
#15
Zachary D Dalebroux
In this issue of Journal of Bacteriology, Rowlett et al. unveil new Escherichia coli circuitry linking membrane glycerophospholipid (GPL) homeostasis to bacterial stress response and adaptation mechanisms. Glycerophospholipids comprise critical components of the dual-membrane envelope of Gram-negative bacteria and participate in many processes. The new evidence suggests that in some instances distinct E. coli GPL molecules function for distinct biochemistry, and bacteria sense perturbations in membrane GPL concentrations to coordinate survival strategies...
April 24, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28439032/inactivation-of-transcriptional-regulators-during-within-household-evolution-of-escherichia-coli
#16
Dagmara I Kisiela, Matthew Radey, Sandip Paul, Stephen Porter, Kseniya Polukhina, Veronika Tchesnokova, Sofiya Shevchenko, Diana Chan, Maliha Aziz, Timothy J Johnson, Lance B Price, James R Johnson, Evgeni V Sokurenko
We analyzed the within-household evolution of two household-associated Escherichia coli strains from pandemic clonal group ST131-H30, using isolates recovered from five individuals within two families, each of which had a distinct strain. Family 1's strain was represented by a urine isolate from the index patient (older sister) with recurrent cystitis, and a blood isolate from her younger sister with fatal urosepsis. Family 2's strain was represented by a urine isolate from the index patient (father) with pyelonephritis and renal abscesses, blood and kidney drainage isolates from the daughter with emphysematous pyelonephritis, and urine and fecal isolates from the mother with cystitis...
April 24, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28438940/emergence-of-plasmid-mediated-mcr-1-gene-in-clinical-kpc-2-producing-klebsiella-pneumoniae-st392-in-brazil
#17
Caio Augusto Martins Aires, Orlando Carlos da Conceição-Neto, Thamirys Rachel Tavares E Oliveira, Carolina Frizzera Dias, Lara Feital Montezzi, Renata Cristina Picão, Rodolpho Mattos Albano, Marise Dutra Asensi, Ana Paula D'Alincourt Carvalho-Assef
Since the first report of the plasmid-mediated colistin resistance mcr-1 gene in Escherichia coli and Klebsiella pneumoniae isolates from China (1), mcr-1 had already spread to most continents, being detected in different species from several sources, including in carbapenemase-producing clinical isolates (2).….
April 24, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28438599/newly-identified-invertebrate-type-lysozyme-splys-i-in-mud-crab-scylla-paramamosain-exhibiting-muramidase-deficient-antimicrobial-activity
#18
Jian Zhou, Shu Zhao, Wen-Hong Fang, Jun-Fang Zhou, Jing-Xiao Zhang, Hongyu Ma, Jiang-Feng Lan, Xin-Cang Li
Lysozymes are widely distributed immune effectors exerting muramidase activity against the peptidoglycan of the bacterial cell wall to trigger cell lysis. However, some invertebrate-type (i-type) lysozymes deficient of muramidase activity still exhibit antimicrobial activity. To date, the mechanism underlying the antimicrobial effect of muramidase-deficient i-type lysozymes remains unclear. Accordingly, this study characterized a novel i-type lysozyme, Splys-i, in the mud crab Scylla paramamosain. Splys-i shared the highest identity with the Litopenaeus vannamei i-type lysozyme (Lvlys-i2, 54% identity) at the amino acid level...
April 21, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28438511/nitric-oxide-inhibits-the-mitochondrial-carnitine-acylcarnitine-carrier-through-reversible-s-nitrosylation-of-cysteine-136
#19
Annamaria Tonazzi, Nicola Giangregorio, Lara Console, Annalisa De Palma, Cesare Indiveri
S-nitrosylation of the mitochondrial Carnitine/Acylcarnitine transporter (CACT) has been investigated on the native and the recombinant proteins reconstituted in proteoliposomes, and on intact mitochondria. The widely-used NO-releasing compound, GSNO, strongly inhibited the antiport measured in proteoliposomes reconstituted with the native CACT from rat liver mitochondria or the recombinant rat CACT over-expressed in E. coli. Inhibition was reversed by the reducing agent dithioerithritol, indicating a reaction mechanism based on nitrosylation of Cys residues of the CACT...
April 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28438456/pirab-protein-from-xenorhabdus-nematophila-hb310-exhibits-a-binary-toxin-with-insecticidal-activity-and-cytotoxicity-in-galleria-mellonella
#20
Qing Yang, Jie Zhang, Tianhui Li, Shen Liu, Ping Song, Ziyan Nangong, Qinying Wang
PirAB (Photorhabdus insect-related proteins, PirAB) toxin was initially found in the Photorhabdus luminescens TT01 strain and has been shown to be a binary toxin with high insecticidal activity. Based on GenBank data, this gene was also found in the Xenorhabdus nematophila genome sequence. The predicted amino acid sequence of pirA and pirB in the genome of X. nematophila showed 51% and 50% identity with those gene sequences from P. luminescens. The purpose of this experiment is to identify the relevant information for this toxin gene in X...
April 21, 2017: Journal of Invertebrate Pathology
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