keyword
MENU ▼
Read by QxMD icon Read
search

RIP1

keyword
https://www.readbyqxmd.com/read/29760953/hyperglycemia-potentiates-a-shift-from-apoptosis-to-rip1-dependent-necroptosis
#1
William D McCaig, Payal S Patel, Sergey A Sosunov, Nicole L Shakerley, Tori A Smiraglia, Miranda M Craft, Katharine M Walker, Matthew A Deragon, Vadim S Ten, Timothy J LaRocca
Apoptosis and necroptosis are the primary modes of eukaryotic cell death, with apoptosis being non-inflammatory while necroptosis is highly inflammatory. We previously demonstrated that, once activated, necroptosis is enhanced by hyperglycemia in several cell types. Here, we determine if hyperglycemia affects apoptosis similarly. We show that hyperglycemia does not enhance extrinsic apoptosis but potentiates a shift to RIP1-dependent necroptosis. This is due to increased levels and activity of RIP1, RIP3, and MLKL, as well as decreased levels and activity of executioner caspases under hyperglycemic conditions following stimulation of apoptosis...
2018: Cell Death Discovery
https://www.readbyqxmd.com/read/29731829/expression-of-receptor-interacting-protein-1-and-receptor-interacting-protein-3-oval-cells-in-a-rat-model-of-hepatocarcinogenesis
#2
Marta Wójcik, Ryszard Bobowiec, Urszula Lisiecka, Anna Śmiech
When apoptosis is suppressed in a neoplastic state, necroptosis may enable an anticancer response. In the present study, the association between apoptosis and necroptosis was assessed in a partial hepatectomy (PH)/diethylnitrosamine (DEN) rat model of hepatocarcinogenesis. Isolated oval cells (OCs) were analysed at 24, 48 and 72 h and at the first and second week of incubation. Phenotypic studies, apoptosis and necroptosis detection and proliferative activity assays were also performed on the OCs. The OCs were isolated from non-neoplastic (PH) and neoplastic (PH/DEN) livers, which expressed receptor interacting protein (RIP) 1 and RIP3...
May 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29725301/necrostatin-1-attenuates-cisplatin-induced-nephrotoxicity-through-suppression-of-apoptosis-and-oxidative-stress-and-retains-klotho-expression
#3
Yichun Ning, Yiqin Shi, Jing Chen, Nana Song, Jieru Cai, Yi Fang, Xiaofang Yu, Jun Ji, Xiaoqiang Ding
Aim: Cisplatin is an effective chemotherapeutic drug, but the application in clinical is greatly limited by its nephrotoxicity. Necrostatin-1 (Nec-1), an inhibitor of RIP1 kinase, has been reported to inhibit RIP-mediated necroptosis. The aim of this study is to detect the protective effects of Nec-1 on the nephrotoxicity of cisplatin and to investigate its renoprotection mechanism. Methods: 8-week-old male C57BL/6 mice were randomly assigned into four groups: Control, Nec-1, Cisplatin, and Cisplatin+Nec-1...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29614353/necrosulfonamide-attenuates-the-spinal-cord-injury-via-necroptosis-inhibition
#4
Yongxiang Wang, Jingcheng Wang, Hua Wang, Xinmin Feng, Yuping Tao, Jiandong Yang, Jun Cai
Spinal cord injury (SCI) is a serious trauma without efficient treatment currently. Necroptosis can be blocked post injury by special inhibitors. To investigate the effects of Necrosulfonamide (NSA) in the treatment of SCI, pathological condition, necroptosis related factors, mitochondrial function and ethological performance were detected. Pathological detection using HE staining was performed first. Reduced lesions and protected neurons were found in the injured spinal cord after treatment with NSA. No obvious toxicity on rat liver, kidney, heart and spleen was detected using HE staining...
March 31, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29611115/c-elegans-based-screen-identifies-lysosome-damaging-alkaloids-that-induce-stat3-dependent-lysosomal-cell-death
#5
Yang Li, Yu Zhang, Qiwen Gan, Meng Xu, Xiao Ding, Guihua Tang, Jingjing Liang, Kai Liu, Xuezhao Liu, Xin Wang, Lingli Guo, Zhiyang Gao, Xiaojiang Hao, Chonglin Yang
Lysosomes are degradation and signaling centers within the cell, and their dysfunction impairs a wide variety of cellular processes. To understand the cellular effect of lysosome damage, we screened natural small-molecule compounds that induce lysosomal abnormality using Caenorhabditis elegans (C. elegans) as a model system. A group of vobasinyl-ibogan type bisindole alkaloids (ervachinines A-D) were identified that caused lysosome enlargement in C. elegans macrophage-like cells. Intriguingly, these compounds triggered cell death in the germ line independently of the canonical apoptosis pathway...
April 2, 2018: Protein & Cell
https://www.readbyqxmd.com/read/29608987/rip1-and-rip3-contribute-to-shikonin-induced-glycolysis-suppression-in-glioma-cells-via-increase-of-intracellular-hydrogen-peroxide
#6
Bin Lu, Zongqi Wang, Ye Ding, Xuanzhong Wang, Shan Lu, Chongcheng Wang, Chuan He, Meihua Piao, Guangfan Chi, Yinan Luo, Pengfei Ge
RIP1 and RIP3 are necroptosis initiators, but their roles in regulation of glycolysis remain elusive. In this study, we found shikonin activated RIP1 and RIP3 in glioma cells in vitro and in vivo, which was accompanied with glycolysis suppression. Further investigation revealed that shikonin-induced decreases of glucose-6-phosphate and pyruvate and downregulation of HK II and PKM2 were significantly prevented when RIP1 or RIP3 was pharmacologically inhibited or genetically knocked down with SiRNA. Moreover, shikonin also triggered accumulation of intracellular H2 O2 and depletion of GSH and cysteine...
March 30, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29605020/targeting-intestinal-epithelial-cell-programmed-necrosis-alleviates-tissue-injury-after-intestinal-ischemia-reperfusion-in-rats
#7
Xiang Li, Yihong Ling, Zhongming Cao, Jiantong Shen, Shaoqian Chen, Weifeng Liu, Baolong Yuan, Shihong Wen
BACKGROUND: Intestinal dysfunction, especially acute pathologies linked to intestinal ischemia/reperfusion (I/R) injury, is profoundly affected by inflammation and improper execution of cell death. Few studies have examined the efficacy of combined strategies in regulated intestinal epithelial necrosis after intestinal I/R. Here, we evaluated the functional interaction between poly (adenosine diphosphate-ribose) polymerase 1 (PARP-1)-induced parthanatos and receptor-interacting protein 1/3 (RIP1/3) kinase-induced necroptosis in the pathophysiological course of acute ischemic intestinal injury...
May 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29581256/puma-amplifies-necroptosis-signaling-by-activating-cytosolic-dna-sensors
#8
Dongshi Chen, Jingshan Tong, Liheng Yang, Liang Wei, Donna B Stolz, Jian Yu, Jianke Zhang, Lin Zhang
Necroptosis, a form of regulated necrotic cell death, is governed by RIP1/RIP3-mediated activation of MLKL. However, the signaling process leading to necroptotic death remains to be elucidated. In this study, we found that PUMA , a proapoptotic BH3-only Bcl-2 family member, is transcriptionally activated in an RIP3/MLKL-dependent manner following induction of necroptosis. The induction of PUMA, which is mediated by autocrine TNF-α and enhanced NF-κB activity, contributes to necroptotic death in RIP3-expressing cells with caspases inhibited...
March 26, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29550818/klotho-reduces-necroptosis-by-targeting-oxidative-stress-involved-in-renal-ischemic-reperfusion-injury
#9
Yingying Qian, Xiangjiang Guo, Lin Che, Xuejing Guan, Bei Wu, Renhua Lu, Mingli Zhu, Huihua Pang, Yucheng Yan, Zhaohui Ni, Leyi Gu
BACKGROUND/AIMS: Klotho is a multifunctional protein expressed predominantly in kidney tubular epithelium. Here, we investigated the protective effects of Klotho on necroptosis in renal ischemic-reperfusion injury (IRI) and the role of oxidative stress in this process. METHODS: Mice were subjected to bilateral renal pedicle clamping. Mouse renal tubular epithelial (TCMK-1) cells were exposed to hypoxia/reoxygenation (H/R) or H2O2. Kidney samples from acute kidney injury (AKI) patients and controls were examined by immunofluorescence...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29546393/bufalin-inhibits-human-breast-cancer-tumorigenesis-by-inducing-cell-death-through-the-ros-mediated-rip1-rip3-parp-1-pathways
#10
Yanlan Li, Xin Tian, Xiaodan Liu, Pengchao Gong
Bufalin, a key active ingredient of the Chinese medicine Chan Su, inhibits breast cancer tumorigenesis in vitro and in vivo. Here, we found that the pan-caspase inhibitor zVAD-fmk failed to inhibit bufalin-induced cell death in MCF-7 and MDA-MB-231 human breast cancer cells, confirming that the cell death induced by bufalin is caspase-independent. Instead, bufalin increased the expression of the necroptosis mediators RIP1 and RIP3. Bufalin-induced cell death was prevented by small molecule inhibitors of RIP1 and poly (ADP-ribose) polymerase-1 (PARP-1) or genetic knockdown of RIP3 by shRNA transfection...
May 3, 2018: Carcinogenesis
https://www.readbyqxmd.com/read/29535128/rip1-hat1-sirt-complex-identification-and-targeting-in-treatment-and-prevention-of-cancer
#11
Vincenzo Carafa, Angela Nebbioso, Francesca Cuomo, Dante Rotili, Gilda Cobellis, Paola Bontempo, Alfonso Baldi, Enrico P Spugnini, Gennaro Citro, Angela Chambery, Rosita Russo, Menotti Ruvo, Paolo Ciana, Luca Maravigna, Jani Shaik, Enrico Radaelli, Pasquale De Antonellis, Domenico Tarantino, Adele Pirolli, Rino Ragno, Massimo Zollo, Hendrik G Stunnenberg, Antonello Mai, Lucia Altucci
Purpose: Alteration in cell death is a hallmark of cancer. A functional role regulating survival, apoptosis, and necroptosis has been attributed to RIP1/3 complexes. Experimental Design: We have investigated the role of RIP1 and the effects of MC2494 in cell death induction, using different methods as flow cytometry, transcriptome analysis, immunoprecipitation, enzymatic assays, transfections, mutagenesis, and in vivo studies with different mice models. Results: Here, we show that RIP1 is highly expressed in cancer, and we define a novel RIP1/3-SIRT1/2-HAT1/4 complex...
March 13, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29530056/novel-smac-mimetic-apg-1387-elicits-ovarian-cancer-cell-killing-through-tnf-alpha-ripoptosome-and-autophagy-mediated-cell-death-pathway
#12
Bao-Xia Li, Heng-Bang Wang, Miao-Zhen Qiu, Qiu-Yun Luo, Han-Jie Yi, Xiang-Lei Yan, Wen-Tao Pan, Lu-Ping Yuan, Yu-Xin Zhang, Jian-Hua Xu, Lin Zhang, Da-Jun Yang
BACKGROUND: Ovarian cancer is a deadly disease. Inhibitors of apoptosis proteins (IAPs) are key regulators of apoptosis and are frequently dysregulated in ovarian cancer. Overexpression of IAPs proteins has been correlated with tumorigenesis, treatment resistance and poor prognosis. Reinstalling functional cell death machinery by pharmacological inhibition of IAPs proteins may represent an attractive therapeutic strategy for treatment of ovarian cancer. METHODS: CCK-8 and colony formation assay was performed to examine cytotoxic activity...
March 12, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29514082/periostin-limits-tumor-response-to-vegfa-inhibition
#13
Ioanna Keklikoglou, Ece Kadioglu, Stefan Bissinger, Benoît Langlois, Axel Bellotti, Gertraud Orend, Carola H Ries, Michele De Palma
Resistance to antiangiogenic drugs limits their applicability in cancer therapy. Here, we show that revascularization and progression of pancreatic neuroendocrine tumors (PNETs) under extended vascular-endothelial growth factor A (VEGFA) blockade are dependent on periostin (POSTN), a matricellular protein expressed by stromal cells. Genetic deletion of Postn in RIP1-Tag2 mice blunted tumor rebounds of M2-like macrophages and αSMA+ stromal cells in response to prolonged VEGFA inhibition and suppressed PNET revascularization and progression on therapy...
March 6, 2018: Cell Reports
https://www.readbyqxmd.com/read/29507626/sarcoma-targeting-peptide-decorated-polypeptide-nanogel-intracellularly-delivers-shikonin-for-upregulated-osteosarcoma-necroptosis-and-diminished-pulmonary-metastasis
#14
Suoyuan Li, Tao Zhang, Weiguo Xu, Jianxun Ding, Fei Yin, Jing Xu, Wei Sun, Hongsheng Wang, Mengxiong Sun, Zhengdong Cai, Yingqi Hua
PURPOSE: Osteosarcoma is the most common primary bone cancer and is notorious for pulmonary metastasis, representing a major threat to pediatric patients. An effective drug targeting osteosarcoma and its lung metastasis is urgently needed. DESIGN: In this study, a sarcoma-targeting peptide-decorated disulfide-crosslinked polypeptide nanogel (STP-NG) was exploited for enhanced intracellular delivery of shikonin (SHK), an extract of a medicinal herb, to inhibit osteosarcoma progression with minimal systemic toxicity...
2018: Theranostics
https://www.readbyqxmd.com/read/29496522/dihydrotanshinone-i-a-natural-product-ameliorates-dss-induced-experimental-ulcerative-colitis-in-mice
#15
Yanling Guo, Xiaxia Wu, Qin Wu, Yuanfu Lu, Jingshan Shi, Xiuping Chen
Ulcerative colitis (UC) is a chronic and relapsing inflammatory disorder of the colon and rectum with increasing morbidity in recent years. 15,16-dihydrotanshinone Ӏ (DHT) is a natural product with multiple bioactivities. In this study, we aimed to investigate the protective effect and potential mechanisms of DHT on UC. Dextran sulfate sodium salt (DSS) was administrated in drinking water for 7 days to induce UC in mice. DHT (10 and 25 mg/kg) significantly alleviated DSS-induced body weight loss, disease activity index (DAI) scores, and improved histological alterations of colon tissues...
April 1, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29485864/discovery-of-7-oxo-2-4-5-7-tetrahydro-6-h-pyrazolo-3-4-c-pyridine-derivatives-as-potent-orally-available-and-brain-penetrating-receptor-interacting-protein-1-rip1-kinase-inhibitors-analysis-of-structure-kinetic-relationships
#16
Masato Yoshikawa, Morihisa Saitoh, Taisuke Katoh, Tomohiro Seki, Simone V Bigi, Yuji Shimizu, Tsuyoshi Ishii, Takuro Okai, Masako Kuno, Harumi Hattori, Etsuro Watanabe, Kumar S Saikatendu, Hua Zou, Masanori Nakakariya, Takayuki Tatamiya, Yoshihisa Nakada, Takatoshi Yogo
We report the discovery of 7-oxo-2,4,5,7-tetrahydro-6 H-pyrazolo[3,4- c]pyridine derivatives as a novel class of receptor interacting protein 1 (RIP1) kinase inhibitors. On the basis of the overlay study between HTS hit 10 and GSK2982772 (6) in RIP1 kinase, we designed and synthesized a novel class of RIP1 kinase inhibitor 11 possessing moderate RIP1 kinase inhibitory activity and P-gp mediated efflux. The optimization of the core structure and the exploration of appropriate substituents utilizing SBDD approach led to the discovery of 22, a highly potent, orally available, and brain-penetrating RIP1 kinase inhibitor with excellent PK profiles...
March 22, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29470982/rip3-deficiency-protects-against-traumatic-brain-injury-tbi-through-suppressing-oxidative-stress-inflammation-and-apoptosis-dependent-on-ampk-pathway
#17
Zai-Ming Liu, Qian-Xue Chen, Zhi-Biao Chen, Dao-Feng Tian, Ming-Chang Li, Jun-Min Wang, Long Wang, Bao-Hui Liu, Shen-Qi Zhang, Fei Li, Hui Ye, Long Zhou
Traumatic brain injury (TBI) is a leading cause of disability and mortality in young adults worldwide. The pathophysiology is not fully understood. Programmed necrosis (necroptosis) is a newly identified mechanism of cell death combining features of both apoptosis and necrosis. Receptor-interacting protein 3 (RIP3) plays an important role in programmed necrosis. However, the effect of RIP3-related pathway in TBI is little to be known. We attempted to explore the significance of RIP3 in regulating TBI in vivo...
May 5, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29464045/pathological-and-functional-significance-of-semaphorin-5a-in-pancreatic-cancer-progression-and-metastasis
#18
Sugandha Saxena, Yuri Hayashi, Lingyun Wu, Mohammad Awaji, Pranita Atri, Michelle L Varney, Abhilasha Purohit, Satyanarayana Rachagani, Surinder K Batra, Rakesh K Singh
Semaphorin-5A (SEMA5A) has differential cell surface expression between normal and cancer cells and represents an attractive target for therapeutic intervention in pancreatic cancer (PC). In this study, we delineated the pathological expression and significance of SEMA5A during PC progression and metastasis. We utilized human tissue microarrays and different PC mouse models (Pdx1-cre; LSL- Kras(G12D) , Pdx1-Cre; LSL-Kras(G12D) ; LSL-p53(R172H) and RIP1-Tag2) to analyze SEMA5A expression during PC progression...
January 19, 2018: Oncotarget
https://www.readbyqxmd.com/read/29452280/sticholysin-ii-mediated-cytotoxicity-involves-the-activation-of-regulated-intracellular-responses-that-anticipates-cell-death
#19
Carmen Soto, Gretchen Bergado, Rancés Blanco, Tania Griñán, Hermis Rodríguez, Uris Ros, Fabiola Pazos, María Eliana Lanio, Ana María Hernández, Carlos Álvarez
Sticholysin II (StII) is a pore-forming toxin of biomedical interest that belongs to the actinoporin protein family. Sticholysins are currently under examination as an active immunomodulating component of a vaccinal platform against tumoral cells and as a key element of a nucleic acids delivery system to cell cytosol. These proteins form pores in the plasma membrane leading to ion imbalance and cell lysis. However, the intracellular mechanisms triggered by actinoporins upon binding to membranes and its consequences for cell death are barely understood...
May 2018: Biochimie
https://www.readbyqxmd.com/read/29436688/necrostatin-1-protects-c2c12-myotubes-from-cocl2-induced-hypoxia
#20
Rui Chen, Jiehua Xu, Yanling She, Ting Jiang, Shanyao Zhou, Huacai Shi, Cheng Li
Necrostatin-1 (Nec-1) is a selective and potent allosteric inhibitor of necroptosis by specifically inhibiting the activity of receptor‑interacting protein (RIP) 1 kinase. The aim of the present study was to determine the effect of Nec‑1 on an anoxia model comprising mouse skeletal C2C12 myotubes. In the present study, a hypoxic mimetic reagent, cobalt chloride (CoCl2), was used to induce hypoxia in C2C12 myotubes. The cytotoxic effects of CoCl2‑induced hypoxia were determined by a Cell Counting kit‑8 assay and flow cytometry...
May 2018: International Journal of Molecular Medicine
keyword
keyword
51830
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"