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Zhenluan Xue, Xiaolong Li, Youbin Li, Mingyang Jiang, Guozhong Ren, Hongrong Liu, Songjun Zeng, Jianhua Hao
Long-lasting persistent luminescent nanoparticles (PLNPs) with efficient near-infrared (NIR) emission have emerged as a new generation of probes for in vivo optical bioimaging owing to their advantages of zero-autofluorescence benefited from the self-sustained emission after excitation, deep penetration depth, and a high signal-to-noise ratio. However, most of the PLNPs are charged by ultraviolet (UV) or visible light, remarkably limiting their applications for in vivo long-term bioimaging. Here we demonstrate 980 nm laser activated upconversion-PLNPs (UC-PLNPs) with efficient NIR emission...
May 19, 2017: Nanoscale
Xu Zhao, Cheng-Xiong Yang, Li-Gong Chen, Xiu-Ping Yan
The integrated functions of diagnostics and therapeutics make theranostics great potential for personalized medicine. Stimulus-responsive therapy allows spatial control of therapeutic effect only in the site of interest, and offers promising opportunities for imaging-guided precision therapy. However, the imaging strategies in previous stimulus-responsive therapies are 'always on' or irreversible 'turn on' modality, resulting in poor signal-to-noise ratios or even 'false positive' results. Here we show the design of dual-stimuli-responsive and reversibly activatable nanoprobe for precision tumour-targeting and fluorescence-guided photothermal therapy...
May 19, 2017: Nature Communications
Jisun Ki, Eunji Jang, Seungmin Han, Moo-Kwang Shin, Byunghoon Kang, Yong-Min Huh, Seungjoo Haam
Various types of nanoprobes have recently been utilized to monitor living organisms by detecting and imaging intracellular biomarkers, such as microRNAs (miRs). We here present a simple one-pot method to prepare stellate gold nanoparticles functionalized with miR-detecting molecular beacons (SGNP-MBs); low pH conditions permitted the rapid-high loading of MBs on the surface of SGNPs. Compared to the conventional gold nanoparticle-based MBs, SGNPs carried a 4.5-fold higher load of MBs and exhibited a 6.4-fold higher cellular uptake...
May 19, 2017: ACS Applied Materials & Interfaces
Xiaojuan Yang, Lin Zhou, Yan Hao, Bin Zhou, Peihui Yang
An erythrocytes (RBCs) cytosensor was first fabricated for in situ analysis of sialic acid (SA) on the cell surface based on a quartz crystal microbalance (QCM). RBCs, as a recognition element, were immobilized on a concanavalin A (ConA)-modified gold chip through the specific recognition between ConA and mannose on the cell surface. 4-Aminobenzeneboronic acid (APBA)-functionalized gold nanoparticles (AuNPs/APBA) were used as a signal amplification nanoprobe for labeling SA on the surface of RBCs. Compared to that of APBA, the frequency response of the cytosensor could be significantly enhanced 18-fold by using a AuNPs/APBA nanoprobe...
May 19, 2017: Analyst
Didier Dréau, Laura Jeffords Moore, Merlis P Alvarez-Berrios, Mubin Tarannum, Pinku Mukherjee, Juan L Vivero-Escoto
Mucin-1 (MUC1), a transmembrane glycoprotein is aberrantly expressed on ~90% of breast cancer and is an excellent target for nanoparticulate targeted imaging. In this study, the development of a dye-doped NIR emitting mesoporous silica nanoparticles platform conjugated to tumor-specific MUC1 antibody (ab-tMUC1-NIR-MSN) for in vivo optical detection of breast adenocarcinoma tissue is reported. The structural properties, the in vitro and in vivo performance of this nanoparticle-based probe were evaluated. In vitro studies showed that the MSN-based optical imaging nanoprobe is non-cytotoxic and targets efficiently mammary cancer cells overexpressing human tMUC1 protein...
December 2016: Journal of Biomedical Nanotechnology
Chang Guo, Minmin Xu, Suying Xu, Leyu Wang
Fluorescence is widely used for cell imaging due to its high sensitivity and rich color choice but limited for in vivo imaging because of its low light penetration. Meanwhile, magnetic resonance imaging (MRI) is widely applied for in vivo diagnosis but not suitable for cell imaging because of its low resolution. Compared to (1)H-MRI, (19)F-MRI is more suitable for clinical application due to its high sensitivity but fabricating (19)F-MRI probes is a great challenge. Therefore, it is highly desirable to develop a dual-modal imaging probe for both cell fluorescence imaging and in vivo(19)F-MRI with high sensitivity and deep penetration...
May 17, 2017: Nanoscale
Na Gao, Wen Yang, Hailiang Nie, Yunqian Gong, Jing Jing, Loujun Gao, Xiaoling Zhang
This paper reports a turn-on theranostic fluorescent nanoprobe P-CDs/HA-Dox obtained by electrostatic assembly of polyethylenimine (PEI)-modified carbon dots (P-CDs) and Hyaluronic acid (HA)-conjugated doxorubicin (Dox) for hyaluronidase (HAase) detection, self-targeted imaging and drug delivery. P-CDs/HA-Dox show weak emission in a physiological environment. By utilizing the high affinity of HA to CD44 receptors overexpressed on many cancer cells, P-CDs/HA-Dox are capable of targeting and penetrating into cancer cells, where they are activated by HAase...
May 10, 2017: Biosensors & Bioelectronics
Savannah Kaye, Zheng Zeng, Mollye Sanders, Krishnan Chittur, Paula M Koelle, Robert Lindquist, Upender Manne, Yongbin Lin, Jianjun Wei
A miniaturized, robust, localized surface plasmon resonance (LSPR)-coupled fiber-optic (FO) nanoprobe providing an integrated and portable solution for detection of DNA hybridization and measurement of DNA concentrations has been demonstrated. The FO nanoprobe was created by constructing arrays of metallic nanostructures on the end facets of optical fibers utilizing nanofabrication technologies, including electron beam lithography and lift-off processes. The LSPR-FO nanoprobe device offers real-time, label-free, and low-sample-volume quantification of single-strand DNA in water with high sensitivity and selectivity, achieving a limit of detection around 10 fM...
May 15, 2017: Analyst
Yanjing Yang, Jin Huang, Xiaohai Yang, Xiaoxiao He, Ke Quan, Nuli Xie, Min Ou, Kemin Wang
A new class of intracellular nanoprobe, termed AuNP-based hairpin-locked-DNAzyme probe, was developed to sense miRNA in living cells. Briefly, it consists of an AuNP and hairpin-locked-DNAzyme strands. In the absence of target miRNA, the hairpin-locked-DNAzyme strand form a hairpin structure by intramolecular hybridization, which could inhibit the catalytic activity of DNAzyme strand and the fluorescence is quenched by the AuNP. However, in the presence of target, the target-probe hybridization can open the hairpin and form the active secondary structure in the catalytic cores to yield an 'active' DNAzyme, which then cleaves the self-strand with the assist of Mg2+...
May 15, 2017: Analytical Chemistry
Weiheng Kong, Di Wu, Lian Xia, Xuefeng Chen, Guoliang Li, Nannan Qiu, Guang Chen, Zhiwei Sun, Jinmao You, Yongning Wu
Recently, α-glucosidase inhibitor has been widely used in clinic for diabetic therapy. In the present study, a facile and sensitive fluorescent assay based on enzyme activated inner filter effect (IFE) on nitrogen-doped carbon dots (CDs) was first developed for the detection of α-glucosidase. The N-doped CDs with green emission were prepared by a one-step hydrothermal synthesis and gave the fluorescence quantum yield of 30%, which were used as the signal output. Through α-glucosidase catalysis, 4-nitrophenol was released from 4-nitrophenyl-α-d-glucopyranoside (NGP)...
June 22, 2017: Analytica Chimica Acta
Zhihao Zhou, Wei Zheng, Jintao Kong, Yan Liu, Ping Huang, Shanyong Zhou, Zhuo Chen, Jianlin Shi, Xueyuan Chen
Persistent luminescence nanoparticles (PLNPs) have shown great promise in the field of biomedicine, but are currently limited by the challenge in the synthesis of high-quality PLNPs with bright persistent luminescence and a long afterglow time. Herein, we report a facile strategy for the synthesis of monodisperse, rechargeable and LED-activated ZnGa2O4 : Cr(3+) near-infrared (NIR) PLNPs based on a modified solvothermal liquid-solid-solution method. The as-synthesized PLNPs are not only flexible for bioconjugation, but could also circumvent the limitation of the weak persistent luminescence and short afterglow time that most PLNPs confronted owing to their rechargeable capability...
May 12, 2017: Nanoscale
Bin Zhou, Mingyao Zhu, Peihui Yang, Peihui Yang
Latent tuberculosis infection (LTBI) is one of the major contributing factors for the high incidence of tuberculosis (TB), and the low contents of LTBI markers in human serum presents a great challenge for the diagnosis of LTBI. Here we reported a novel electrochemiluminescence (ECL) sensing platform for the precise analysis of multiple LTBI markers, interferon-gamma (IFN-γ) and interleukin (IL)-2. In this approach, self-prepared carbon quantum dots (CQDs) and luminol were integrated onto gold nanoparticles (AuNPs), which were further enriched on the surface of magnetic bead (MB) to create two solid-phase ECL nanoprobes (MB@Au@CQDs and MB@Au@luminol) for improving the detection sensitivity efficiently...
May 12, 2017: ACS Applied Materials & Interfaces
Sujin Ahn, Hyunung Yu, Seong Ho Kang
Carcinoembryonic antigen (CEA) is a glycoprotein associated with colorectal carcinomas and is commonly used as a clinical tumor marker. Enhanced detection sensitivity for the assay of CEA molecules was achieved on a plasmonic nanoimmunosensor by wavelength-dependent transmission grating (TG)-based total internal reflection scattering microscopy (TIRSM). The plasmonic nanoparticles were placed in an evanescent field layer on a glass nanoimmunosensor that produced evanescent wave scattering by the total internal reflection of light from two lasers...
May 4, 2017: Biosensors & Bioelectronics
Deng-Chyang Wu, Kai-Yi Wang, Sophie S W Wang, Ching-Min Huang, Yi-Wei Lee, Michael Isaac Chen, Szu-An Chuang, Shu-Hua Chen, Ying-Wei Lu, Chun-Cheng Lin, Ka-Wo Lee, Wen-Hung Hsu, Kun-Pin Wu, Yu-Ju Chen
We reported an integrated platform to explore serum protein variant pattern in cancer and its utility as a new class of biomarker panel for diagnosis. On the model study of serum amyloid A (SAA), we employed nanoprobe-based affinity mass spectrometry for enrichment, identification and quantitation of SAA variants from serum of 105 gastric cancer patients in comparison with 54 gastritis patients, 54 controls, and 120 patients from other cancer. The result revealed surprisingly heterogeneous and most comprehensive SAA bar code to date, which comprises 24 SAA variants including SAA1- and SAA2-encoded products, polymorphic isoforms, N-terminal-truncated forms, and three novel SAA oxidized isotypes, in which the variant-specific peptide sequence were also confirmed by LC-MS/MS...
May 11, 2017: Proteomics
Limin Yang, Yuanyuan Chen, Wei Pan, Hongyu Wang, Na Li, Bo Tang
Excess alcohol consumption and the associated development of alcoholic liver disease (ALD) is a major public health challenge in the world-wide. Since patients with the severe stages of ALD no longer benefit from clinical therapies, early warning of ALD holds significant promise for increasing the cure rate of ALD. Herein, we develop a bicolor fluorescent nanoprobe for dynamically monitoring the conversion process of alcohol-induced fatty liver to steatohepatitis in vivo through simultaneous imaging of microRNA 155 and osteopontin mRNA, which are related to fatty liver and steatohepatitis respectively...
May 11, 2017: Analytical Chemistry
Miao-Miao Yin, Ping Dong, Wen-Qi Chen, Shi-Ping Xu, Liyun Yang, Feng-Lei Jiang, Yi Liu
Noble metal nanoclusters (NCs) show great promise as nanoprobes for bioanalysis and cellular imaging in biological applications due to ultra-small size, good photophysical properties and excellent biocompatibility. In order to achieve a comprehensive understanding of possible biological implications, a series of spectroscopic measurements were conducted under different temperatures to investigate the interactions of Au NCs (~1.7 nm) with three model plasmatic proteins (human serum albumin (HSA), γ-globulins and transferrin)...
May 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
Wen Zhou, Ya-Fei Tian, Bin-Cheng Yin, Bang-Ce Ye
Simultaneous detection of cancer biomarkers holds great promise for the early diagnosis of cancer. In the present work, an ultrasensitive and reliable surface-enhanced Raman scattering (SERS) sensor has been developed for simultaneous detection of multiple liver cancer related microRNA (miRNA) biomarkers. We first proposed a novel strategy for the synthesis of nanogap-based SERS nanotags by modifying gold nanoparticles (AuNPs) with thiolated DNA and nonfluorescent small encoding molecules. We also explored a simple approach to a green synthesis of hollow silver microspheres (Ag-HMSs) with bacteria as templates...
May 17, 2017: Analytical Chemistry
Qian Zhang, Ting Yin, Guo Gao, Joseph G Shapter, Weien Lai, Peng Huang, Wen Qi, Jie Song, Daxiang Cui
Core@shell magnetic nanoparticles (core@shell MNPs) are attracting widespread attention due to their enhancement properties for potential applications in hyperthermia treatment, magnetic resonance imaging (MRI), diagnostics, and so forth. Herein, we developed a facile thermal decomposition method for controllable synthesis of a superparamagnetic, monodispersed core@shell structure (Co@Mn = CoFe2O4@MnFe2O4) with uniform size distribution (σ < 5%, dc ≈ 15 nm). The CoFe2O4 core could enhance magnetic anisotropy, and the MnFe2O4 shell could improve the magnetization value...
May 18, 2017: ACS Applied Materials & Interfaces
Abhignyan Nagesetti, Alexandra Rodzinski, Emmanuel Stimphil, Tiffanie Stewart Chooda Khanal, Ping Wang, Rakesh Guduru, Ping Liang, Irina Agoulnik, Jeffrey Horstmyer, Sakhrat Khizroev
Magnetoelectric (ME) nanoparticles (MENs) intrinsically couple magnetic and electric fields. Using them as nuclear magnetic resonance (NMR) sensitive nanoprobes adds another dimension for NMR detection of biological cells based on the cell type and corresponding particle association with the cell. Based on ME property, for the first time we show that MENs can distinguish different cancer cells among themselves as well as from their normal counterparts. The core-shell nanoparticles are 30 nm in size and were not superparamagnetic...
May 3, 2017: Scientific Reports
Qian Chen, Chao Liang, Xiaoqi Sun, Jiawen Chen, Zhijuan Yang, He Zhao, Liangzhu Feng, Zhuang Liu
Abnormal H2O2 levels are closely related to many diseases, including inflammation and cancers. Herein, we simultaneously load HRP and its substrate, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), into liposomal nanoparticles, obtaining a Lipo@HRP&ABTS optical nanoprobe for in vivo H2O2-responsive chromogenic assay with great specificity and sensitivity. In the presence of H2O2, colorless ABTS would be converted by HRP into the oxidized form with strong near-infrared (NIR) absorbance, enabling photoacoustic detection of H2O2 down to submicromolar concentrations...
May 8, 2017: Proceedings of the National Academy of Sciences of the United States of America
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