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Keunsuk Kim, Jeong-Wook Oh, Young Kwang Lee, Jiwoong Son, Jwa-Min Nam
Amplification- and enzyme-free quantification of DNA at ultra-low concentrations, on the orders of 10-1,000 targets, is highly beneficial, but is extremely challenging. To address this challenge, true detection signals should be reliably discriminated from false or noise signals. Here, we developed the dynamically associating and dissociating nanodimer analysis (ADNA) method that allows for collecting maximum detection signals from true target-binding events while keeping nonspecific signals at minimum level...
June 23, 2017: Angewandte Chemie
Yu Wang, Cheng-Xiong Yang, Xiu-Ping Yan
The development of the multimodal probes is of great importance for bioimaging application. Herein, we report the fabrication of a functional nanocomposite from near-infrared (NIR) persistent luminescent nanoparticles (PLNPs) and Gd2O3 as a multimodal probe for in vivo NIR persistent luminescence and magnetic resonance (MR) imaging. Small-sized monodispersed NIR ZnGa2O4:Cr(3+) PLNPs (ca. 15 nm) were prepared as the NIR persistent luminescence source by a hydrothermal method while hyaluronic acid (HA) functionalized Gd2O3 (HA-Gd2O3) was synthesized as the MR contrast agent via a biomineralization approach...
June 22, 2017: Nanoscale
Liewei Wen, Sihua Yang, Junping Zhong, Quan Zhou, Da Xing
Multifunctional nanoparticle-mediated imaging and therapeutic techniques are promising modalities for accurate localization and targeted treatment of cancer in clinical settings. Thermoacoustic (TA) imaging is highly sensitive to detect the distribution of water, ions or specific nanoprobes and provides excellent resolution, good contrast and superior tissue penetrability. TA therapy is a potential non-invasive approach for the treatment of deep-seated tumors. In this study, human serum albumin (HSA)-functionalized superparamagnetic iron oxide nanoparticle (HSA-SPIO) is used as a multifunctional nanoprobe with clinical application potential for MRI, TA imaging and treatment of tumor...
2017: Theranostics
Dae-Young Kim, Surendra Shinde, Gajanan Ghodake
The functional nanoparticles with specific molecular probe appear to be a promising approach for developing colorimetric nanosensor. In this work, we have synthesized tryptophan capped gold nanoparticles (AuNPs) and used to establish colorimetric detection of magnesium (Mg(2+)). The colorimetric response of the AuNPs toward Mg(2+) was noticed with naked eyes, and spectral changes were monitored by using UV-Vis spectrophotometer. The detection response was rapid (less than 1 min), with a detection limit (LOD) about 0...
June 21, 2017: Scientific Reports
Yanzhu Liu, Liucheng Mao, Xinhua Liu, Meiying Liu, Dazhuang Xu, Ruming Jiang, Fengjie Deng, Yongxiu Li, Xiaoyong Zhang, Yen Wei
Aggregation-induced emission (AIE) active fluorescent polymeric nanoparticles (FPNs) have recently emerged as the promising nanoprobes for biological imaging for their intensive fluorescence, good photostability, desirable biocompatibility and well designability of structure and optical properties. Herein, we proposed a novel strategy for fabrication of AIE-active FPNs through the post modification of synthetic copolymers to form Schiff base. The size, morphology, optical properties and biocompatibility as well as cell uptake behavior were evaluated in detailed...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Jingfang Shangguan, Jin Huang, Dinggeng He, Xiaoxiao He, Kemin Wang, Runzhi Ye, Xue Yang, Taiping Qing, Jinlu Tang
Measuring the levels of Fe3+ in human body has attracted considerable attention for health monitoring as it plays an essential element in many physiological processes. In this work, we reported a selective fluorescent nanoprobe for Fe3+ detection in biological samples based on ultra-bright N/P co-doped carbon dots. By employing adenosine 5'-triphosphate (ATP) as the carbon, nitrogen and phosphorus source, the N/P co-doped carbon dots could be simply prepared through hydrothermal treatment. The obtained carbon dots exhibited high quantum yields up to 43...
June 19, 2017: Analytical Chemistry
Yan-Hong Yuan, Yi-Di Wu, Bao-Zhu Chi, Shao-Hua Wen, Ru-Ping Liang, Jian-Ding Qiu
We report a sensor combining two distinguishable magnetic nanoprobes (DNA1/Fe3O4 NPs/Thi and DNA2/Fe3O4 NPs/Fc) with target-triggered hybridization chain reaction (HCR) strategy for the simultaneous detection of microRNA-141 (miR-141) and microRNA-21 (miR-21). In the presence of targets, the thiol-modified hairpin capture probes (HCP1 and HCP2) specifically hybridize with miR-141 and miR-21 on a gold electrode, leading to the conformation change of HCP1 and HCP2, respectively. The conformation change subsequently triggers HCR to generate plentiful bonding sequences of magnetic nanoprobes...
June 11, 2017: Biosensors & Bioelectronics
Juntian Qu, Xinyu Liu
In this paper, we investigate the impact of vacuum chamber conditions (cleanliness level and vacuum pressure) and imaging parameters (magnification and acceleration voltage) of scanning electron microscopy (SEM) on the contact resistance of two-point in-situ nanoprobing of nanomaterials. Using two typical types of conductive nanoprobe, two-point nanoprobing is performed on silicon nanowires, during which changing trends of the nanoprobing contact resistance with the SEM chamber conditions and imaging parameters are quantified...
June 15, 2017: Nanotechnology
Lihua Zhi, Xiaofan Zeng, Hao Wang, Jun Hai, Xiangliang Yang, Baodui Wang, Yanhong Zhu
The development of sensitive and reliable methods to monitor the presence of mercuric ions in cells and organisms is of great importance to biological research and biomedical applications. In this work, we propose a strategy to construct a solar-driven nanoprobe using a 3D Au@MoS2 heterostructure as a photocatalyst and rhodamine B (RhB) as a fluorescent and color change reporter molecule for monitoring Hg2+ in living cells and animals. The sensing mechanism is based on the photoinduced electron formation of gold amalgam in the 3D Au@MoS2 heterostructure under visible light illumination...
June 14, 2017: Analytical Chemistry
Yaning Tan, Xiaoxia Hu, Meng Liu, Xinwen Liu, Xiaobo Lv, Zhihao Li, Jie Wang, Quan Yuan
The simultaneous quantitation of multiple steroid hormones in real-time is of great importance for medical diagnosis. In this study, a portable hormone biosensor based on duplex molecular recognition coupled with a signal-amplified substrate was successfully developed for the simultaneous visualization and quantitation of multiple steroid hormones. Aptamer-functionalized upconversion nanoparticles (UCNPs) with different emission peaks are immobilized on the photonic crystal (PC) substrate as the nanoprobes, leading to the specific and simultaneous assay of multiple steroid hormones...
June 13, 2017: Chemistry: a European Journal
Jin-Lei Li, Jun-Peng Shi, Cheng-Cheng Wang, Peng-Hui Li, Zhen-Feng Yu, Hong-Wu Zhang
Until now, the afterglow emissions of most developed near infrared (NIR)-emitting persistent luminescent nanoparticles (NPLNPs) were located at approximately 700 nm, at the edge of the first tissue transparency window (from 650 to 900 nm), which resulted in relatively low tissue penetration and signal-to-noise ratio (SNR) for in vivo imaging. Herein, 5 nm ZnSn2O4:Cr,Eu (ZSO) NPLNPs with NIR afterglow emission at 800 nm are synthesized via a direct aqueous-phase synthesis method. The longer NIR afterglow emission of ZSO NPLNPs can easily penetrate approximately 3 cm of pork tissue...
June 13, 2017: Nanoscale
Cheolhwan Jeong, Hyung-Mo Kim, So Yeon Park, Myeong Geun Cha, Sung-Jun Park, San Kyeong, Xuan-Hung Pham, Eunil Hahm, Yuna Ha, Dae Hong Jeong, Bong-Hyun Jun, Yoon-Sik Lee
We report magnetic silver nanoshells (M-AgNSs) that have both magnetic and SERS properties for SERS-based detection. The M-AgNSs are composed of hundreds of Fe₃O₄ nanoparticles for rapid accumulation and bumpy silver shell for sensitive SERS detection by near-infrared laser excitation. The intensity of the SERS signal from the M-AgNSs was strong enough to provide single particle-level detection. We obtained much stronger SERS signal intensity from the aggregated M-AgNSs than from the non-aggregated AgNSs...
June 13, 2017: Nanomaterials
Pegah Moradi Khaniabadi, Daryoush Shahbazi-Gahrouei, Amin Malik Shah Abdul Majid, Mohammad Suhaimi Jaafar, Bita Moradi Khaniabadi, Saghar Shahbazi-Gahrouei
Background: Magnetic resonance imaging (MRI) plays an essential role in molecular imaging by delivering the contrast agent into targeted cancer cells. The aim of this study was to evaluate the C595 monoclonal antibody-conjugated superparamagnetic iron oxide nanoparticles (SPIONs-C595) for the detection of breast cancer cell (MCF-7). Methods: The conjugation of monoclonal antibody and nanoparticles was confirmed using X-ray diffraction, transmission electron microscopy, and photon correlation spectroscopy...
June 11, 2017: Iranian Biomedical Journal
Su Hyun Hong, Yao Sun, Chu Tang, Kai Cheng, Ruiping Zhang, Quli Fan, Liying Xu, Daijuan Huang, Anthony Zhao, Zhen Cheng
Development of a chelator-free and biocompatible platform for facile construction of gadolinium3+ (Gd3+) loaded nanoparticle-based probe for in vivo magentic resonance imaging (MRI) is still challenging. Herein, a biocompatible Gd3+ loaded melanin dot (Gd-M-dot) has been easily preppared without the use of ay chelators, and the resulted nanoprobe exhibited good loading efficiency for Gd3+, high stability, and higher T1 relaxivity compared with the commercial contrast agent, Gd-DOTA. Furthermore, Gd-M-dot showed unique photoacoustic (PA) property, and high PA imaging signal could be observed after 1 h injection in small animal models...
June 8, 2017: Bioconjugate Chemistry
Ju Lu, Shenfei Zong, Zhuyuan Wang, Chen Chen, Yizhi Zhang, Yiping Cui
A new yolk-shell type nanoprobe for super-resolution imaging is demonstrated. Using the proposed nanoprobe and single molecule localization based super resolution imaging strategy, intracellular nanoparticle tracking and super-resolution imaging are realized. The localization precision is about 50 nm and single-molecule localization microscopy using the proposed nanoprobe requires only one single excitation laser and no specific imaging buffer.
June 8, 2017: Nanotechnology
Jiali Zhu, Shan Sun, Kai Jiang, Yuhui Wang, Wenqing Liu, Hengwei Lin
Herein, a highly sensitive and selective fluorimetric nanoprobe for peroxynitrite (ONOO(-)) detection based on photoinduced electron transfer (PET) from ferrocene (Fc) to carbon dots (CDs) is reported. The nanoprobe (named CDs-Fc) can be facilely constructed through covalently conjugating CDs and ferrocenecarboxylic acid. Further studies reveal that the energy level of highest occupied molecular orbital (HOMO) of the CDs is lowered with the addition of ONOO(-) due to its oxidation and nitration capabilities...
May 31, 2017: Biosensors & Bioelectronics
Xiangyu Meng, Guotai Yang, Fulai Li, Taobo Liang, Weihua Lai, Hengyi Xu
A novel sandwich strategy was designed to detect Staphylococcus aureus. The strategy is based on an antibacterial agent that captures bacterial cells and a fluorescein-labeled antibody that acts as the signal-output probe. Vancomycin (Van), which exerts a strong antibacterial effect on Gram-positive bacteria, was utilized as a molecular recognition agent to detect pathogenic bacteria. To effectively concentrate S. aureus, we used bovine serum albumin (BSA) as the amplification carrier to modify magnetic beads (MBs), which were then functionalized with Van...
June 16, 2017: ACS Applied Materials & Interfaces
Yang Zhao, Jing Peng, Jingjin Li, Ling Huang, Jingyi Yang, Kai Huang, Hewen Li, Ning Jiang, Shaokuan Zheng, Xuening Zhang, Yuanjie Niu, Gang Han
Biocompatibility, targeting, and clearance are key challenges in the design of new MRI contrast agents. Herein, we report on a tumor-targeting, gadolinium biomineralized human transferrin (Tf) protein-based nanoparticle (Gd@Tf NP) for MRI use. As compared to the conventionally used gadolinium chelates, the resultant Gd@Tf NPs possess outstanding chemical stability and exhibited superior longitudinal relaxation. More importantly, our MR images show that Gd@Tf indeed retained the natural tumor targeting ability and the subsequent tumor retrieval biofunctions of Tf...
June 5, 2017: Nano Letters
Jiaze Tang, Ning Huang, Xiang Zhang, Tao Zhou, Ying Tan, Jiangli Pi, Li Pi, Si Cheng, Huzhi Zheng, Yuan Cheng
The extent of resection is a significant prognostic factor in glioma patients. However, the maximum safe resection level is difficult to determine due to the inherent infiltrative character of tumors. Recently, fluorescence-guided surgery has emerged as a new technique that allows safe resection of glioma. In this study, we constructed a new kind of quantum dot (QD)-labeled aptamer (QD-Apt) nanoprobe by conjugating aptamer 32 (A32) to the QDs surface, which can specially bind to the tumors. A32 is a single-stranded DNA capable of binding to the epidermal growth factor receptor variant III (EGFRvIII) specially distributed on the surface of glioma cells...
2017: International Journal of Nanomedicine
Qi Yue, Xihui Gao, Yang Yu, Yang Li, Wei Hua, Kun Fan, Ren Zhang, Jun Qian, Liang Chen, Cong Li, Ying Mao
Surgery is a mainstay to treat malignant brain tumors. However, due to the infiltrative nature of these tumors, it is a great challenge for surgeons to accurately identify and excise all the tumor foci. EGFRvIII, a variant of epidermal growth factor receptor (EGFR), is found in 20% of glioblastoma cases, which is the brain tumor with the highest malignancy. In this study, we developed an EGFRvIII-targeted nanoprobe to guide glioblastoma surgery by pre-operatively defining the tumor boundary via magnetic resonance imaging (MRI) and intra-operatively guiding resection by surface-enhanced resonance Raman scattering (SERRS) imaging...
June 1, 2017: Nanoscale
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