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Rajni Bala, Sherry Mittal, Rohit K Sharma, Nishima Wangoo
In the present study, we report a highly sensitive, rapid and low cost colorimetric monitoring of malathion (an organophosphate insecticide) employing a basic hexapeptide, malathion specific aptamer (oligonucleotide) and silver nanoparticles (AgNPs) as a nanoprobe. AgNPs are made to interact with the aptamer and peptide to give different optical responses depending upon the presence or absence of malathion. The nanoparticles remain yellow in color in the absence of malathion owing to the binding of aptamer with peptide which otherwise tends to aggregate the particles because of charge based interactions...
February 5, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Zhouzhou Bao, Yuqing Zhang, Ziyang Tan, Xia Yin, Wen Di, Jian Ye
The sentinel lymph node (SLN) biopsy is gaining in popularity as a procedure to investigate the lymphatic metastasis of malignant tumors. The commonly used techniques to identify the SLNs in clinical practice are blue dyes-guided visualization, radioisotope-based detection and near-infrared fluorescence imaging. However, all these methods have not been found to perfectly fit the clinical criteria with issues such as short retention time in SLN, poor spatial resolution, autofluorescence, low photostability and high cost...
February 10, 2018: Biomaterials
Lifu Xiao, Abdul K Parchur, Timothy A Gilbertson, Anhong Zhou
G-protein-coupled receptor 120 (GPR120), as a member of the rhodopsin family of G-protein-coupled receptors, has been shown to function as a sensor for dietary fat in the gustatory and digestive systems. Its specific role in the chemoreception of fatty acids, which is thought to be crucial in understanding the mechanism surrounding the control of fat intake and, accordingly, in the treatment of obesity, remains unclear. Here we report a novel surface-enhanced Raman spectroscopy (SERS)-fluorescence bimodal microscopic technique for detection and imaging of GPR120 in single living cells...
2018: Analytical Methods: Advancing Methods and Applications
Doudou Lou, Yanyan Tian, Yu Zhang, Junjie Yin, Ting Yang, Chuan He, Ming Ma, Wei Yu, Ning Gu
We found that the peroxidase-like activity of gold nanoparticles (GNPs) followed the Michaelis-Menten kinetic model and was dependent on environmental pH and temperature, which was very similar to natural Horseradish Peroxidase (HRP). However, unlike HRP, which needs a lower H2O2 concentration with a very narrow range to reach a maximum reaction rate and avoid enzyme poisoning, GNPs have very high activity, even at an H2O2 concentration two orders of magnitude higher than HRP. It was demonstrated that H2O2 treatment could enhance the peroxidase-like activity of GNPs, resulting thus in the activity increase in a circular catalytic reaction by the reduplicative use of GNPs...
February 1, 2018: Journal of Nanoscience and Nanotechnology
Attasith Parnsubsakul, Sukunya Oaew, Werasak Surareungchai
Zwitterionic nanoparticles are typically utilized as nanoprobes and delivery vehicles in nanomedicine and therapeutics due to their resistance to interferences. Their high stability also shows great potential to be applied in sensing applications. Here, we report a selective, sensitive and rapid colorimetric sensing of nickel ions (Ni 2+ ) using zwitterionic polypeptide, EKEKEKPPPPC (EK) 3 , capped gold nanoparticles (AuNP-(EK) 3 ). By taking advantage of the alternate carboxylic (-COOH)/amine (-NH 2 ) groups, the zwitterionic peptide can function dually by being able to sense metal ions and maintain colloidal stability...
February 15, 2018: Nanoscale
Hao He, Christopher B Howard, Yinghui Chen, Shihui Wen, Gungun Lin, Jiajia Zhou, Kristofer James Thurecht, Dayong Jin
Upconversion nanoparticles (UCNPs) are new optical probes for biological applications. To realize specific biomolecular recognition for diagnosis and imaging, the key lies in developing a stable and easy-to-use bioconjugation method for antibody modification. Current methods are not yet satisfactory regarding conjugation time, stability, and binding efficiency. Here we report a facile and high-yield approach based on a bispecific antibody (BsAb), free of chemical reaction steps. One end of the BsAb is designed to recognize methoxy polyethylene glycol (mPEG) coated UCNPs, and the other end of the BsAb is to recognize the cancer antigen biomarker...
February 14, 2018: Analytical Chemistry
Yuling Tong, Xitong Huang, Mi Lu, Bo-Yang Yu, Jiangwei Tian
The development of well-designed nanoprobes for specific imaging of multiple biomarkers in renal cells will afford beneficial information related to the transmutation process of drug-induced kidney injury (DIKI). However, the most reported nanoprobes for DIKI detection were dependent on single-signal output and lack of kidney targeting. In this work, we reported a renal cell targeting and dual-signal nanoprobe by encapsulating Brite 670 and Dabcyl-KFFFDEVDK-FAM into low molecular weight chitosan nanoparticle...
February 14, 2018: Analytical Chemistry
Xiaoyue Jiang, Shenfei Zong, Chen Chen, Yizhi Zhang, Zhuyuan Wang, Yiping Cui
As a novel fluorescent nanomaterial, gold-carbon quantum dots (GCDs) possess high biocompatibility and can be easily synthesized by a microwave-assisted method. Owing to their small sizes and unique optical properties, GCDs can be applied to imaging of biological targets, such as cells, exosomes and other organelles. In this study, GCDs were used for fluorescence imaging of exosomes. Tumor-specific antibodies are attached to the GCDs, forming exosome specific nanoprobes. The nanoprobes can label exosomes via immuno-reactions and thus facilitate fluorescent imaging of exosomes...
February 13, 2018: Nanotechnology
Yanting Shen, Lijia Liang, Shuqin Zhang, Dianshuai Huang, Rong Deng, Jing Zhang, Huixin Qu, Shuping Xu, Chongyang Liang, Weiqing Xu
Subcellular organelles, e.g., nucleus, mitochondria and lysosome, are the vital organelles with responsibilities that maintain cell operation and metabolism. Owing to their roles in energy production and programmed cell death, these organelles have become prime therapeutic targets in different diseases and states. In this study, biocompatible, organelle-targeting nanoprobes were developed by modifying gold nanorods (AuNRs) with specific targeting peptides. These nanoprobes were employed to directly profile subcellular biomolecules and vital organelles in situ by surface-enhanced Raman scattering (SERS) spectroscopy...
February 13, 2018: ACS Applied Materials & Interfaces
Tang Gao, Shuanglian Wang, Wuwu Lv, Mian Liu, Hongliang Zeng, Zhu Chen, Jie Dong, Ziping Wu, Xueping Feng, Wenbin Zeng
Herein, a novel self-assembled nanoprobe for the long-term tracking of the nucleoli of cancer cells and for differentiating between clinical breast cancer tissues and para-carcinoma tissues has been developed.
February 12, 2018: Chemical Communications: Chem Comm
Mingshu Xiao, Tiantian Man, Changfeng Zhu, Hao Pei, Jiye Shi, Li Li, Xiangmeng Qu, Xizhong Shen, Jiang Li
MicroRNAs (miRNAs) play significant regulatory roles in physiologic and pathologic processes and are considered as important biomarkers for disease diagnostics and therapeutics. Simple, fast, sensitive, and selective detection of miRNAs, however, is challenged by their short length, low abundance, susceptibility to degradation, and homogenous sequence. Here we develop MoS2-loaded molecular beacons (MBs) nanoprobes for highly sensitive and selective detection of miRNAs based on duplex-specific nuclease-mediated signal amplification (DSNMSA)...
February 12, 2018: ACS Applied Materials & Interfaces
Tuğba Köker, Nathalie Tang, Chao Tian, Wei Zhang, Xueding Wang, Richard Martel, Fabien Pinaud
The in cellulo assembly of plasmonic nanomaterials into photo-responsive probes is of great interest for many bioimaging and nanophotonic applications but remains challenging with traditional nucleic acid scaffolds-based bottom-up methods. Here, we address this quandary using split-fluorescent protein (FP) fragments as molecular glue and switchable Raman reporters to assemble gold or silver plasmonic nanoparticles (NPs) into photonic clusters directly in live cells. When targeted to diffusing surface biomarkers in cancer cells, the NPs self-assemble into surface-enhanced Raman-scattering (SERS) nanoclusters having hot spots homogenously seeded by the reconstruction of full-length FPs...
February 9, 2018: Nature Communications
Shuming Zhang, Bixia Lin, Ying Yu, Yujuan Cao, Manli Guo, Lingling Shui
Ratiometric fluorescent probes could eliminate the influence from experimental factors and improve the detection accuracy. In this article, a ratiometric nanoprobe was constructed based on silver nanoclusters (AgNCs) with nitrogen-doped carbon dots (NCDs) and used for the detection of biothiols. The fluorescence peak of AgNCs was observed at 650nm with excitation wavelength at 370nm. In order to construct the ratiometric fluorescent probe, NCDs with the excitation and emission wavelengths at 370nm and 450nm were selected...
February 1, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Kusum Vats, Ashis Kumar Satpati, Rohit Sharma, Haladhar Dev Sarma, Drishty Satpati, Ashutosh Dash
This study explores the potential of 177Lu-labeled carbon nanospheres as radio-nanoprobes for molecular imaging and therapy. The carboxyl functionalized surface of carbon nanospheres (CNS) was conjugated with [Gly-Gly-Gly-c(Asn-Gly-Arg)], G3-cNGR peptide through amide bond for targeting tumor vasculature and with [2-(4-Aminobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid], p-NH2-Bz-DOTA for chelation with 177Lu. The nanosphere-peptide conjugate, DOTA-CNS-cNGR, was characterized by dynamic light scattering and zeta potential measurements, IR and UV experiments and did not show any in vitro cytotoxicity...
February 1, 2018: Journal of Pharmaceutical and Biomedical Analysis
Yu Liu, Xiaolin Lv, Heng Liu, Zijian Zhou, Jianpan Huang, Shenglan Lei, Shuhui Cai, Zhong Chen, Yanli Guo, Zhiwei Chen, Xi Zhou, Liming Nie
Stimuli-responsive nanoprobes that integrate multi-modal imaging capacities are highly desirable for precise tumor visualization. Herein, novel porous gold nanocluster-decorated manganese monoxide nanocomposites (MnO@Au NCs) were synthesized via a facile approach. The porous gold nanocluster layer was germinated on the surface of the as-prepared MnO@DMSA NPs through simple reduction of chloroauric acid in the presence of hydroxylamine hydrochloride. The MnO@Au NCs could be effectively internalized by tumor cells and slowly release Mn2+ ions within the acidic tumor microenvironment, improving the visualization of the tumor morphology...
February 7, 2018: Nanoscale
Sabrina Alessio Camacho, Regivaldo Gomes Sobral Filho, Pedro Henrique Benites Aoki, Carlos Jose Leopoldo Constantino, Alexandre G Brolo
Zika virus (ZIKV) was considered a public health emergency of international concern after the 2015 outbreak. Serological tests based on immunoassay platforms is one of the methods applied on the diagnosis of ZIKV and dengue virus (DENV). However, the high limits of detection (LOD) and the cross-reactivity between ZIKV and DENV are still limitations in immunological tests. In order to tackle these issues, we have designed an immune-specific assay based on surface-enhanced Raman scattering (SERS) nanoprobes. Gold shell-isolated nanoparticles (Au-SHINs) were synthesized with 100 nm Au nanoparticles and 4 nm silica shell thickness coated with Nile Blue (Raman reporter)...
February 7, 2018: ACS Sensors
Gonca Bülbül, Akhtar Hayat, Fatima Mustafa, Silvana Andreescu
Functional nanomaterials with fluorescent or quenching abilities are important for the development of molecular probes for detection and studies of nucleic acids. Here, we describe a new class of molecular nanoprobes, the NanoCeracQ that uses nanoceria particles as a nanoquencher of fluorescent oligonucleotides for rapid and sensitive detection of DNA sequences and hybridization events. We show that nanoceria forms stable and reversible bionanoconjugates with oligonucleotides and can specifically recognize and detect DNA sequences in a single step...
February 5, 2018: Scientific Reports
Ge Xu, Qinglong Yan, Xiaoguang Lv, Ying Zhu, Kai Xin, Ben Shi, Rongchen Wang, Jian Chen, Wei Gao, Ping Shi, Chunhai Fan, Chunchang Zhao, He Tian
Fluorescent probes in the second near-infrared window (NIR-II) allow high-resolution bioimaging with deep-tissue penetration. However, existing NIR-II materials often have poor signal-to-background ratios due to the lack of target specificity. Herein, we devised an activatable NIR-II nanoprobe for visualizing colorectal cancers. This designed probe possessed H2S-activated ratiometric fluorescence and light-up NIR-II emission of 900-1300 nm. By using this activatable and target specific probe for deep-tissue imaging of H2S-rich colon cancer cells, we realized accurate location of colorectal tumors in animal models...
February 2, 2018: Angewandte Chemie
Didem Şen Karaman, Mirkka P Sarparanta, Jessica M Rosenholm, Anu J Airaksinen
Recent progress in the development of silica- and silicon-based multimodality imaging nanoprobes has advanced their use in image-guided drug delivery, and the development of novel systems for nanotheranostic and diagnostic applications. As biocompatible and flexibly tunable materials, silica and silicon provide excellent platforms with high clinical potential in nanotheranostic and diagnostic probes with well-defined morphology and surface chemistry, yielding multifunctional properties. In vivo imaging is of great value in the exploration of methods for improving site-specific nanotherapeutic delivery by silica- and silicon-based drug-delivery systems...
February 1, 2018: Advanced Materials
Ke Luan, Ruiqian Meng, Changfu Shan, Jing Cao, Jianguo Jia, Wei-Sheng Liu, Yu Tang
Rapid, sensitive and selective quantitative detection of pyridine dicarboxylic acid (DPA) as biomarker of anthrax spores is in great demand since anthrax spores are lethal to human beings and animals highly and also the potential biological warfare agents. Herein, we prepared a ratiometric fluorescence lanthanide functionalized micelle nanoprobe by "one-pot" self-assembly, with an amphiphilic ligand containing β-diketone derivative which can "immobilize" terbium ions through the coordination interaction and a fluorophore as fluorescence reference (FR)...
February 1, 2018: Analytical Chemistry
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