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Qin Wang, Shengrui Zhang, Yaogang Zhong, Xiao-Feng Yang, Zheng Li, Hua Li
Selenocysteine (Sec) carries out the majority of the functions of the various Se-containing species in vivo. Thus, it is of great importance to develop sensitive and selective assays to detect Sec. Herein, a carbon-dot-based fluorescent turn-on probe for highly selective detection of selenol in living cells is presented. The highly photoluminescent carbon dots that emit yellow-green fluorescence (Y-G-CDs; λmax = 520 nm in water) were prepared by using m-aminophenol as carbon precursor through a facile solvothermal method...
February 7, 2017: Analytical Chemistry
Sumaira Hanif, Hailing Liu, Ming Chen, Pir Muhammad, Yue Zhou, Jiao Cao, Saud Asif Ahmed, Jingjuan Xu, Xinghua Xia, Hongyuan Chen, Kang Wang
It is challenging to develop a robust nanoprobe for real-time operational and accurate detection of heavy metals in single cells. Fe-CN coordination chemistry has been well studied to determine the structural characteristics of hemeproteins by different techniques. However, the frequently used cyanide ligands are inorganic molecules that release cyanide anion under particular conditions and cause cyanide poisoning. In the present study, organic cyanide (4-mercaptobenzonitrile, MBN) was utilized for the first time in developing a facile nanoprobe based on surface-enhanced Raman scattering (SERS) for quantitative detection of hemeproteins (oxy-Hb) and trivalent iron (Fe(3+)) ions...
February 7, 2017: Analytical Chemistry
Jing Su, Dongfang Wang, Lena Nörbel, Jianlei Shen, Zhihan Zhao, Yanzhi Dou, Tianhuan Peng, Jiye Shi, Sanjay Mathur, Chunhai Fan, Shiping Song
Uniform silver-containing metal nanostructures with strong and stable surface-enhanced Raman scattering (SERS) signals hold great promise for developing ultrasensitive probes for biodetection. Nevertheless, the direct synthesis of such ready-to-use nanoprobes remains extremely challenging. Herein we report a DNA-mediated gold-silver nanomushroom with interior nanogaps directly synthesized and used for multiplex and simultaneous SERS detection of various DNA and RNA targets. The DNA involved in the nanostructures can act as not only gap DNA (mediated DNA) but also probe DNA (hybridized DNA), and DNA's involvement enables the nanostructures to have the inherent ability to recognize DNA and RNA targets...
February 9, 2017: Analytical Chemistry
Xueqin Jiang, Peijun Tang, Panpan Gao, Yu Shrike Zhang, Changqing Yi, Jianhua Zhou
Ozone (O3) would be harmful to human skin for its strong oxidizing property, especially when stratum corneum or corneal epithelium is wounded. Imaging the penetration and distribution of ozone at depth is beneficial for studying the influence of ozone on skin or eyes. Here, we introduced a facile method for three-dimensional (3D) imaging of the penetration of O3 into the anterior chamber of an isolated crucian carp eye by using optical coherence tomography (OCT) combined with gold triangular nanoprisms (GTNPs) as the contrast agent and molecular probe...
February 2, 2017: Analytical Chemistry
Sungi Kim, Jeong-Eun Park, Woosung Hwang, Jinyoung Seo, Young-Kwang Lee, Jae-Ho Hwang, Jwa-Min Nam
Multiplexed real-time analysis on multiple interacting molecules and particles is needed to obtain information on binding patterns between multiple ligands and receptors, specificity of bond formations and interacting pairs in a complex medi-um, often found in chemical and biological systems, and difference in binding affinity and kinetics for different binding pairs in one solution. In particular, multiplexed profiling of microRNA (miRNA) in a reliable, quantitative manner is of great demand for the use of miRNA in cell biology, biosensing and clinical diagnostic applications, and accurate diagnosis of cancers with miRNA is not possible without detecting multiple miRNA sequences in a highly specific manner...
February 9, 2017: Journal of the American Chemical Society
Janina Kneipp
Surface-enhanced Raman scattering (SERS) probes and labels exploit surface-enhanced Raman signatures of specifically chosen reporter molecules and intrinsic biomolecules in the plasmonic near field of gold and silver nanostructures. They offer several advantages over other optical labels and sensors with respect to sensitivity and selectivity, mobility, and biocompatibility. Here, the multifunctionality and versatility of SERS labels that come from the vibrational spectroscopic information and their ability to act as nanoprobes of a biological environment are discussed...
February 8, 2017: ACS Nano
Zixi Hu, Juntao Tan, Zongqiang Lai, Rong Zheng, Jianhong Zhong, Yiwei Wang, Xiaoxue Li, Nuo Yang, Jieping Li, Wei Yang, Yong Huang, Yongxiang Zhao, Xiaoling Lu
PURPOSE: The purpose of this study is to develop a simple, effective method to label hepatoma cells with aptamers and then detect them using fluorescent silica nanoparticles (FSNPs). METHOD: Streptavidin was conjugated to carboxyl-modified fluorescein isothiocyanate (FITC)-doped silica nanoparticles which were prepared by the reverse microemulsion method. The resulting streptavidin-conjugated fluorescent silica nanoparticles (SA-FSNPs) were mixed with hepatoma cells that had been labeled with biotin-conjugated aptamer TLS11a (Bio-TLS11a)...
December 2017: Nanoscale Research Letters
Sriram Sridhar, Sachin Mishra, Miklós Gulyás, Parasuraman Padmanabhan, Balázs Gulyás
Nanomaterials have gained tremendous significance as contrast agents for both anatomical and functional preclinical bio-imaging. Contrary to conventional medical practices, molecular imaging plays an important role in exploring the affected cells, thus providing precision medical solutions. It has been observed that incorporating nanoprobes improves the overall efficacy of the diagnosis and treatment processes. These nano-agents and tracers are therefore often incorporated into preclinical therapeutic and diagnostic applications...
February 1, 2017: International Journal of Molecular Sciences
Jinchang Yin, Chaorui Li, Deqi Chen, Jiajun Yang, Huan Liu, Wenyong Hu, Yuanzhi Shao
We report a class of multi-functional core-shell nanoarchitectures, consisting of silica nanospheres as the core and Gd2O3:Dy(3+) nanocrystals as the ultra-thin shell, that enable unique multi-color living cell imaging and remarkable in vivo magnetic resonance imaging. These types of targeted cell imaging nanoarchitectures can be used as a variety of fluorescence nanoprobes due to the multi-color emissions of the Gd2O3:Dy(3+) nanophosphor. We also proposed a strategy of modulating core-shell structure design to achieve an enhanced magnetic resonance contrast ability of Gd2O3 nanoagents, and the classical Solomon-Bloembergen-Morgan theory was applied to explicate the mechanism underlying the enhancement...
February 3, 2017: Physical Chemistry Chemical Physics: PCCP
Myung Kim, Young Hun Seo, Youngsun Kim, Jeongyun Heo, Woo-Dong Jang, Sang Jun Sim, Sehoon Kim
A nanoreactor approach based on the amphiphilic assembly of various molecules offers a chance to finely engineer the internal reaction medium to enable highly selective and sensitive detection of H2S in biological media, being useful for microscopic imaging of cellular processes and in vitro diagnostics with blood samples.
February 3, 2017: Chemical Communications: Chem Comm
Sa Huang, Peng Chen, Chen Xu
Nowadays, cancer has become a serious threat to public health worldwide. Early and precise diagnosis is critical for the detection, monitoring and management of cancer. Herein, a facile strategy for developing a fluorescence/MRI dual-modal nanoprobe (FA-PEI-NaGdF4:Eu nanoparticles) has been reported for targeted cancer cell imaging. The fluorescence/MRI nanoprobes have strong fluorescence and the high signal-to-noise ratio, which is suitable for the fluorescence imaging. Moreover, the FA-PEI-NaGdF4:Eu nanoprobes have high longitudinal relaxivity, making them suitable for T1-weighted MR imaging...
April 1, 2017: Talanta
Rubén Ruiz-González, Roger Bresolí-Obach, Òscar Gulías, Montserrat Agut, Huguette Savoie, Ross W Boyle, Santi Nonell, Francesca Giuntini
A biocompatible fluorescent nanoprobe for singlet oxygen ((1) O2 ) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green(®) (SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to (1) O2 . In contrast to its molecular counterpart, the optimum SOSG-based nanoprobe, which we call NanoSOSG, is readily internalized by E...
February 2, 2017: Angewandte Chemie
Ruirui Qiao, Hongyu Qiao, Yan Zhang, Yabin Wang, Chongwei Chi, Jie Tian, Lifang Zhang, Feng Cao, Mingyuan Gao
Owing to the high mortality rate of cardiovascular diseases, developing novel noninvasive diagnostic methods becomes urgent and mandatory. It is well-known that the rupture of vulnerable plaques directly leads to deadly consequences. However, differentiating vulnerable plaques from stable plaques remains challenging in the clinic. In the current study, osteopontin (OPN), a secreted biomarker associated with macrophages and foamy macrophages, was selected as a target for identifying the vulnerable plaques. A dual modality imaging probe was constructed by covalently attaching an OPN antibody to NaGdF4:Yb,Er@NaGdF4 upconversion nanoparticles...
January 31, 2017: ACS Nano
Xin Chen, Huanhuan Zhu, Xin Huang, Peisong Wang, Fulei Zhang, Wei Li, Guang Chen, Bingdi Chen
As the most common endocrine malignancy with a high incidence, thyroid cancer lacks a dual-modal imaging method for precise diagnosis. An accurate and multimodal imaging system is pivotal to solve this problem. Herein, dual-modality fluorescence/Computed Tomography (CT) iodinated gold nanoclusters for malignant thyroid cancer visualization have been recently fabricated. In this study, innovative iodinated gold nanoclusters (AuNCs@BSA-I) were synthesized via Bovine serum albumin (BSA) and chloramine-T. AuNCs@BSA-I not only possess an ultra-small size and brilliant biocompatibility but also exhibit excellent fluorescence/CT imaging properties...
January 25, 2017: Nanoscale
Takanori Morikawa, Kazumichi Yokota, Makusu Tsutsui, Masateru Taniguchi
Reliable ways of DNA sequencing by tunnelling current require low-noise and high-speed measurements of tunnelling current in aqueous environments. Here we report a use of insulator-protected nanoprobes for achieving 7.6 pA rms noise at 50 kHz sampling rates in an electrolyte solution. We utilized dielectric-covered mechanically-controllable break junctions to create a pair of nano-exposed insulator/Au coaxial electrodes closely separated by 1 nm. We observed insulator layer thickness dependent noise levels, which was attributed to decreased capacitance through replacing capacitive electric double layers by the low-k dielectrics...
January 24, 2017: Nanoscale
Cuiling Zhang, Caiping Ding, Guohua Zhou, Qin Xue, Yuezhong Xian
DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn(2+)) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS2 nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay...
March 8, 2017: Analytica Chimica Acta
Xiuli Fu, Lingxin Chen, Jaebum Choo
No abstract text is available yet for this article.
January 3, 2017: Analytical Chemistry
Aaron H Colby, Samantha M Berry, Ann M Moran, Kristine Amber Pasion, Rong Liu, Yolonda L Colson, Nelson Ruiz-Opazo, Mark W Grinstaff, Victoria L M Herrera
A current challenge in the treatment of peritoneal carcinomatosis is the inability to detect, visualize, and resect small or microscopic tumors of pancreatic, ovarian, or mesothelial origin. In these diseases, the completeness of primary tumor resection is directly correlated with patient survival, and hence, identifying small sub-millimeter tumors (i.e., disseminated disease) is critical. Thus, new imaging techniques and probes are needed to improve cytoreductive surgery and patient outcomes. Highly fluorescent rhodamine-labeled expansile nanoparticles (HFR-eNPs) are described for use as a visual aid during cytoreductive surgery of pancreatic carcinomatosis...
February 1, 2017: ACS Nano
Kuncheng Yang, Shanshan Wang, Yingyi Wang, Hong Miao, Xiaoming Yang
Herein, carbon dots (CD@Papain) from papain have been originally synthesized in aqueous solution along with a quantum yield of 6.2%, and showed cyan fluorescence at 435nm. Meanwhile, papain-templated Au nanoclusters (AuNCs@Papain) were prepared with the same precursor. On the basis of the two types of nanomaterials, CD@Papain and AuNCs@Papain have been designed to assemble as one nanosensor (termed as CD-AuNCs@Papain) through a typical cross-linking reaction. Significantly, it not only emitted the dual-emission fluorescent signals with the same excitation, but also can assay multiple targets through exhibiting cyan, yellow, and red emission respectively...
January 7, 2017: Biosensors & Bioelectronics
Xing Zhou, Xiangjun Zhang, Songling Han, Yin Dou, Mengyu Liu, Lin Zhang, Jiawei Guo, Qing Shi, Genghao Gong, Ruibing Wang, Jiang Hu, Xiaohui Li, Jianxiang Zhang
Targeting of nanoparticles to distant diseased sites after oral delivery remains highly challenging due to the existence of many biological barriers in the gastrointestinal tract. Here we report targeted oral delivery of diverse nanoparticles in multiple disease models, via a "Trojan horse" strategy based on a bioinspired yeast capsule (YC). Diverse charged nanoprobes including quantum dots (QDs), iron oxide nanoparticles (IONPs), and assembled organic fluorescent nanoparticles can be effectively loaded into YC through electrostatic force-driven spontaneous deposition, resulting in different diagnostic YC assemblies...
January 17, 2017: Nano Letters
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