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https://www.readbyqxmd.com/read/28212523/redox-regulation-in-metabolic-programming-and-inflammation
#1
REVIEW
Helen R Griffiths, Dan Gao, Chathyan Pararasa
Energy metabolism and redox state are intrinsically linked. In order to mount an adequate immune response, cells must have an adequate and rapidly available energy resource to migrate to the inflammatory site, to generate reactive oxygen species using NADPH as a cofactor and to engulf bacteria or damaged tissue. The first responder cells of the innate immune response, neutrophils, are largely dependent on glycolysis. Neutrophils are relatively short-lived, dying via apoptosis in the process of bacterial killing through production of hypochlorous acid and release of extracellular NETs...
February 12, 2017: Redox Biology
https://www.readbyqxmd.com/read/28211702/antimicrobial-effect-of-biocompatible-silicon-nanoparticles-activated-by-therapeutic-ultrasound
#2
Svetlana N Shevchenko, Markus Burkhardt, Eugene V Sheval, Ulyana A Natashina, Christina Große, Alexander L Nikolaev, Alexander V Gopin, Ute Neugebauer, Andrew A Kudryavtsev, Vladimir Sivakov, Liubov Andreevna Osminkina
In this study, we report on a method for suppressing of Escherichia coli (E.coli) vitality by means of therapeutic ultrasound irradiation (USI) using biocompatible silicon nanoparticles as cavitation sensitizers. Silicon nanoparticles without (SiNPs) and with polysaccharide (dextran) coating (DSiNPs) were used. Both types of nanoparticles were non-toxic to Hep 2 cells up to concentration of 2 mg/ml. The treatment of bacteria with nanoparticles and application of 1 W/cm2 USI resulted in reduction of their viability up to 35% and 72% for SiNPs and DSiNPs, respectively...
February 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28210633/potential-usefulness-of-streptococcus-pneumoniae-extracellular-membrane-vesicles-as-antibacterial-vaccines
#3
Chi-Won Choi, Edmond Changkyun Park, Sung Ho Yun, Sang-Yeop Lee, Seung Il Kim, Gun-Hwa Kim
The secretion of extracellular membrane vesicles (EMVs) is a common phenomenon that occurs in archaea, bacteria, and mammalian cells. The EMVs of bacteria play important roles in their virulence, biogenesis mechanisms, and host cell interactions. Bacterial EMVs have recently become the focus of attention because of their potential as highly effective vaccines that cause few side effects. Here, we isolated the EMVs of Streptococcus pneumoniae and examined their potential as new vaccine candidates. Although the S...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28209804/long-chain-acyl-coa-synthetase-isoforms-differ-in-preferences-for-eicosanoid-species-and-long-chain-fatty-acids
#4
Eric L Klett, Shufen Chen, Alekhya Yechoor, Fred B Lih, Rosalind A Coleman
Because the signaling eicosanoids, epoxyeicosatrienoic acids (EETs) and hydroxyeicosatetraenoic acids (HETEs), are esterified to membrane phospholipids, we asked which long-chain acyl-CoA synthetase (ACSL) isoforms would activate these molecules and whether the apparent fatty acid substrate preferences of each ACSL isoform might differ depending on whether it was assayed in mammalian cell membranes or as a purified bacterial recombinant protein. We found that all five ACSL isoforms were able to use EETs and HETEs as substrates and showed by LC-MS that ACSLs produce EET-CoAs...
February 16, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28209374/design-synthesis-and-evaluation-of-novel-pyrazolo-pyrimido-4-5-d-pyrimidine-derivatives-as-potent-antibacterial-and-biofilm-inhibitors
#5
Lingala Suresh, P Sagar Vijay Kumar, Y Poornachandra, C Ganesh Kumar, G V P Chandramouli
An efficient four-component reaction of 6-amino-1,3-dimethyluracil, N,N-dimethylformamide dimethylacetal, 1-phenyl-3-(4-substituted-phenyl)-4-formyl-1H-pyrazoles and aromatic amines was conducted in the presence of [Bmim]FeCl4 ionic liquid as a promoting medium. This strategy provided a convenient route without any additional catalyst or metal salt under mild conditions. All the synthesized pyrazolo-pyrimido[4,5-d]pyrimidines derivatives were evaluated for their antibacterial, minimum bactericidal concentration (MBC), biofilm inhibition, intracellular ROS accumulation and protein leakage activities...
February 3, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28207828/the-wide-spectrum-high-biocidal-potency-of-bioxy-formulation-when-dissolved-in-water-at-different-concentrations
#6
Dori Dagher, Ken Ungar, Richard Robison, Fadi Dagher
Traditional surface disinfectants that have long been applied in medicine, animal husbandry, manufacturing and institutions are inconvenient at best and dangerous at worst. Moreover, some of these substances have adverse environmental impacts: for example, quaternary ammonium compounds ("quats") are reproductive toxicants in both fish and mammals. Halogens are corrosive both to metals and living tissues, are highly reactive, can be readily neutralized by metals, and react with organic matter to form toxic, persistent by-products such as dioxins and furans...
2017: PloS One
https://www.readbyqxmd.com/read/28207269/a-biocompatible-injectable-hydrogel-with-potent-wound-healing-and-antibacterial-properties
#7
Jiaul Hoque, Relekar G Prakash, Krishnamoorthy Paramanandham, Bibek R Shome, Jayanta Haldar
Two component injectable hydrogels that cross-link in-situ have been used as non-invasive wound-filling devices, i.e., sealants. These materials carry a variety of functions at the wound sites such as seal leaks, cease unwanted bleeding, bind tissues together, and assist in wound healing process. However, commonly used sealants typically lack antibacterial properties. Since bacterial infection at the wound site is very common, bioadhesive materials with intrinsic antibacterial properties are urgently required...
February 16, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28204885/de-bugging-and-maximizing-plant-cytochrome-p450-production-in-escherichia-coli-with-c-terminal-gfp-fusions
#8
Ulla Christensen, Dario Vazquez-Albacete, Karina M Søgaard, Tonja Hobel, Morten T Nielsen, Scott James Harrison, Anders Holmgaard Hansen, Birger Lindberg Møller, Susanna Seppälä, Morten H H Nørholm
Cytochromes P450 (CYP) are attractive enzyme targets in biotechnology as they catalyze stereospecific C-hydroxylations of complex core skeletons at positions that typically are difficult to access by chemical synthesis. Membrane bound CYPs are involved in nearly all plant pathways leading to the formation of high-value compounds. In the present study, we systematically maximize the heterologous expression of six different plant-derived CYP genes in Escherichia coli, using a workflow based on C-terminal fusions to the green fluorescent protein...
February 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28203673/amphipathic-guanidine-embedded-glyoxamide-based-peptidomimetics-as-novel-antibacterial-agents-and-biofilm-disruptors
#9
Shashidhar Nizalapur, Onder Kimyon, Eugene Yee, Kitty Ho, Thomas Berry, Mike Manefield, Charles G Cranfield, Mark Willcox, David StC Black, Naresh Kumar
Antimicrobial resistance in bacteria is becoming increasingly prevalent, posing a critical challenge to global health. Bacterial biofilm formation is a common resistance mechanism that reduces the effectiveness of antibiotics. Thus, the development of compounds that can disrupt bacterial biofilms is a potential strategy to combat antimicrobial resistance. We report herein the synthesis of amphipathic guanidine-embedded glyoxamide-based peptidomimetics via ring-opening reactions of N-naphthoylisatins with amines and amino acids...
February 16, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28199899/role-of-outer-membrane-protein-t-in-pathogenicity-of-avian-pathogenic-escherichia-coli
#10
Hassan M A Hejair, Jiale Ma, Yingchu Zhu, Min Sun, Wenyang Dong, Yue Zhang, Zihao Pan, Wei Zhang, Huochun Yao
An outer membrane protein T (OmpT) could play a vital role in the pathogenesis of the neonatal meningitis Escherichia coli (NMEC) in human and animals. However, whether ompT plays a role in avian pathogenic E. coli (APEC) infection remains unclear. In this study we evaluated the potential of ompT in APEC pathogenesis. An ompT gene was deleted from APEC mutant strain (TW-XM) was constructed and characterized. The inactivation of ompT reduced significantly the adherence and invasion capabilities of APEC to mouse brain microvascular endothelial cell (BMEC) bEnd...
January 27, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28199365/click-chemistry-approach-to-study-mycoloylated-proteins-evidence-for-porb-and-porc-porins-mycoloylation-in-corynebacterium-glutamicum
#11
Hanane Issa, Emilie Huc-Claustre, Thamila Reddad, Nolwenn Bonadé Bottino, Maryelle Tropis, Christine Houssin, Mamadou Daffé, Nicolas Bayan, Nathalie Dautin
Protein mycoloylation is a recently identified, new form of protein acylation. This post-translational modification consists in the covalent attachment of mycolic acids residues to serine. Mycolic acids are long chain, α-branched, β-hydroxylated fatty acids that are exclusively found in the cell envelope of Corynebacteriales, a bacterial order that includes important genera such as Mycobacterium, Nocardia or Corynebacterium. So far, only 3 mycoloylated proteins have been identified: PorA, PorH and ProtX from C...
2017: PloS One
https://www.readbyqxmd.com/read/28198615/antibacterial-activities-of-graphene-oxide-molybdenum-disulfide-nanocomposite-films
#12
Tae In Kim, Buki Kwon, Jonghee Yoon, Ick-Joon Park, Gyeong Sook Bang, YongKeun Park, Yeon-Soo Seo, Sung-Yool Choi
Two-dimensional (2D) nanomaterials, such as graphene-based materials and transition metal dichalcogenide (TMD) nanosheets, are promising materials for biomedical applications owing to their remarkable cytocompatibility and physicochemical properties. Based on their potent antibacterial properties, 2D materials have potential as antibacterial films, wherein the 2D nanosheets are immobilized on the surface and the bacteria may contact with the basal planes of 2D nanosheets dominantly rather than contact with the sharp edges of nanosheets...
February 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28198457/identification-of-a-novel-type-iii-secretion-associated-outer-membrane-bound-protein-from-xanthomonas-campestris-pv-campestris
#13
Lei Li, Rui-Fang Li, Zhen-Hua Ming, Guang-Tao Lu, Ji-Liang Tang
Many bacterial pathogens employ the type III secretion system (T3SS) to translocate effector proteins into eukaryotic cells to overcome host defenses. To date, most of our knowledge about the T3SS molecular architecture comes from the studies on animal pathogens. In plant pathogens, nine Hrc proteins are believed to be structural components of the T3SS, of which HrcC and HrcJ form the outer and inner rings of the T3SS, respectively. Here, we demonstrated that a novel outer membrane-bound protein (HpaM) of Xanthomonas campestris pv...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28198449/recombinant-vacuolar-iron-transporter-family-homologue-pfvit-from-human-malaria-causing-plasmodium-falciparum-is-a-fe-2-h-exchanger
#14
Paola Labarbuta, Katie Duckett, Catherine H Botting, Osama Chahrour, John Malone, John P Dalton, Christopher J Law
Vacuolar iron transporters (VITs) are a poorly understood family of integral membrane proteins that can function in iron homeostasis via sequestration of labile Fe(2+) into vacuolar compartments. Here we report on the heterologous overexpression and purification of PfVIT, a vacuolar iron transporter homologue from the human malaria-causing parasite Plasmodium falciparum. Use of synthetic, codon-optimised DNA enabled overexpression of functional PfVIT in the inner membrane of Escherichia coli which, in turn, conferred iron tolerance to the bacterial cells...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28197161/biotic-and-abiotic-stresses-activate-different-ca-2-permeable-channels-in-arabidopsis
#15
Xiao-Qiang Cao, Zhong-Hao Jiang, Yan-Yan Yi, Yi Yang, Li-Ping Ke, Zhen-Ming Pei, Shan Zhu
To survive, plants must respond rapidly and effectively to various stress factors, including biotic and abiotic stresses. Salinity stress triggers the increase of cytosolic free Ca(2+) concentration ([Ca(2+)]i) via Ca(2+) influx across the plasma membrane, as well as bacterial flg22 and plant endogenous peptide Pep1. However, the interaction between abiotic stress-induced [Ca(2+)]i increases and biotic stress-induced [Ca(2+)]i increases is still not clear. Employing an aequorin-based Ca(2+) imaging assay, in this work, we investigated the [Ca(2+)]i changes in response to flg22, Pep1, and NaCl treatments in Arabidopsis thaliana...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28196960/repair-of-a-bacterial-small-%C3%AE-barrel-toxin-pore-depends-on-channel-width
#16
Gisela von Hoven, Amable J Rivas, Claudia Neukirch, Martina Meyenburg, Qianqian Qin, Sapun Parekh, Nadja Hellmann, Matthias Husmann
Membrane repair emerges as an innate defense protecting target cells against bacterial pore-forming toxins. Here, we report the first paradigm of Ca(2+)-dependent repair following attack by a small β-pore-forming toxin, namely, plasmid-encoded phobalysin of Photobacterium damselae subsp. damselae In striking contrast, Vibrio cholerae cytolysin, the closest ortholog of phobalysin, subverted repair. Mutational analysis uncovered a role of channel width in toxicity and repair. Thus, the replacement of serine at phobalysin´s presumed channel narrow point with the bulkier tryptophan, the corresponding residue in Vibrio cholerae cytolysin (W318), modulated Ca(2+) influx, lysosomal exocytosis, and membrane repair...
February 14, 2017: MBio
https://www.readbyqxmd.com/read/28196959/membrane-remodeling-by-a-bacterial-phospholipid-methylating-enzyme
#17
Linna Danne, Meriyem Aktas, Andreas Unger, Wolfgang A Linke, Ralf Erdmann, Franz Narberhaus
Membrane deformation by proteins is a universal phenomenon that has been studied extensively in eukaryotes but much less in prokaryotes. In this study, we discovered a membrane-deforming activity of the phospholipid N-methyltransferase PmtA from the plant-pathogenic bacterium Agrobacterium tumefaciens PmtA catalyzes the successive three-step N-methylation of phosphatidylethanolamine to phosphatidylcholine. Here, we defined the lipid and protein requirements for the membrane-remodeling activity of PmtA by a combination of transmission electron microscopy and liposome interaction studies...
February 14, 2017: MBio
https://www.readbyqxmd.com/read/28196878/global-characterization-of-protein-secretion-from-human-macrophages-following-non-canonical-caspase-4-5-inflammasome-activation
#18
Martina B Lorey, Katariina Rossi, Kari K Eklund, Tuula A Nyman, Sampsa Matikainen
Gram-negative bacteria are associated with a wide spectrum of infectious diseases in humans. Inflammasomes are cytosolic protein complexes that are assembled when the cell encounters pathogens or other harmful agents. The non-canonical caspase-4/5 inflammasome is activated by gram-negative bacteria-derived lipopolysaccharide (LPS) and by endogenous oxidized phospholipids. Protein secretion is a critical component of the innate immune response. Here we have used label-free quantitative proteomics to characterize global protein secretion in response to non-canonical inflammasome activation upon intracellular LPS recognition in human primary macrophages...
February 14, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28196833/characterization-of-the-activation-of-small-gtpases-by-their-gefs-on-membranes-using-artificial-membrane-tethering
#19
François Peurois, Simon Veyron, Yann Ferrandez, Ilham Ladid, Sarah Benabdi, Mahel Zeghouf, Gérald Peyroche, Jacqueline Cherfils
Attachment of active, GTP-bound small GTPases to membranes by post-translational lipid modifications is pivotal for their ability to process and propagate information in cells. However, generating and manipulating lipidated GTPases has remained difficult, which has limited our quantitative understanding of their activation by GEFs and their termination by GAPs. Here we replaced the lipid modification by a histidine tag in eleven full-length, human small GTPases belonging to the Arf, Rho and Rab families, which allowed to tether them to nickel-lipid containing membranes and characterize the kinetics of their activation by GEFs...
February 14, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28195220/induction-of-invertebrate-larval-settlement-different-bacteria-different-mechanisms
#20
Marnie L Freckelton, Brian T Nedved, Michael G Hadfield
Recruitment via settlement of pelagic larvae is critical for the persistence of benthic marine populations. For many benthic invertebrates, larval settlement occurs in response to surface microbial films. Larvae of the serpulid polychaete Hydroides elegans can be induced to settle by single bacterial species. Until now, only Pseudoalteromonas luteoviolacea had been subjected to detailed genetic and mechanistic studies. To determine if the complex structures, termed tailocins, derived from phage-tail gene assemblies and hypothesized to be the settlement cue in P...
February 14, 2017: Scientific Reports
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