Zixin Bao, Yuting Gao, Yitong Song, Ning Ding, Wei Li, Qiong Wu, Xiaomei Zhang, Yang Zheng, Junming Li, Xuejun Hu
The production of N -linked glycoproteins in genetically engineered Escherichia coli holds significant potential for reducing costs, streamlining bioprocesses, and enhancing customization. However, the construction of a stable and low-cost microbial cell factory for the efficient production of humanized N -glycosylated recombinant proteins remains a formidable challenge. In this study, we developed a glyco-engineered E. coli chassis to produce N -glycosylated proteins with the human-like glycan Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,3-GlcNAc-, containing the human glycoform Gal-β-1,4-GlcNAc-β-1,3-...
2024: Frontiers in Bioengineering and Biotechnology