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https://www.readbyqxmd.com/read/28642371/electrostatic-interactions-between-the-ctx-phage-minor-coat-protein-and-the-bacterial-host-receptor-tola-drives-the-pathogenic-conversion-of-vibrio-cholerae
#1
Laetitia Houot, Romain Navarro, Matthieu Nouailler, Denis Duché, Françoise Guerlesquin, Roland Lloubes
Vibrio cholerae is a natural inhabitant of aquatic environments and converts to a pathogen upon infection by a filamentous phage, CTXφ, that transmits the cholera toxin encoding genes. This toxigenic conversion of V. cholerae has evident implication in both genome plasticity and epidemic risk, but the early stages of the infection have not been thoroughly studied. CTXφ transit across the bacterial periplasm requires binding between the minor coat protein named pIII and a bacterial inner-membrane receptor, TolA, which is part of the conserved Tol-Pal molecular motor...
June 22, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28640743/glucose-consumption-in-carbohydrate-mixtures-by-phosphotransferase-system-mutants-of-escherichia-coli
#2
Tian Xia, Neeraj Sriram, Sarah A Lee, Ronni Altman, Jeffrey L Urbauer, Elliot Altman, Mark A Eiteman
Escherichia coli lacking the glucose phosphotransferase system (PTS), mannose PTS and glucokinase are supposedly unable to grow on glucose as the sole carbon source (Curtis SJ, Epstein W. J Bacteriol 1975;122:1189-1199). We report that W ptsG manZ glk (ALS1406) grows slowly on glucose in media containing glucose with a second carbon source: ALS1406 metabolizes glucose after that other carbon source, including arabinose, fructose, glycerol, succinate or xylose, is exhausted. Galactose is an exception to this rule, as ALS1406 simultaneously consumes both galactose and glucose...
June 22, 2017: Microbiology
https://www.readbyqxmd.com/read/28639287/exploration-of-conformational-changes-in-lactose-permease-upon-sugar-binding-and-proton-transfer-through-coarse-grained-simulations
#3
Yead Jewel, Prashanta Dutta, Jin Liu
Escherichia coli lactose permease (LacY) actively transports lactose and other galactosides across cell membranes through lactose/H(+) symport process. Lactose/H(+) symport is a highly complex process that involves sugar translocation, H(+) transfer, as well as large-scale protein conformational changes. The complete picture of lactose/H(+) symport is largely unclear due to the complexity and multiscale nature of the process. In this work, we develop the force field for sugar molecules compatible with PACE, a hybrid and coarse-grained force field that couples the united-atom protein models with the coarse-grained MARTINI water/lipid...
June 22, 2017: Proteins
https://www.readbyqxmd.com/read/28636907/epr-spectroscopy-targets-structural-changes-in-the-e-%C3%A2-coli-membrane-fusion-cusb-upon-cu-i-binding
#4
Aviv Meir, Ahmad Abdelhai, Yoni Moskovitz, Sharon Ruthstein
Bacterial cells have developed sophisticated systems to deal with the toxicity of metal ions. Escherichia coli CusCFBA is a complex efflux system, responsible for transferring Cu(I) and Ag(I) ions; this system, located in the periplasm, involves four proteins, CusA, CusB, CusC, and CusF. CusA, CusB, and CusC are connected to one another in an oligomerization ratio of 3:6:3 CusA/CusB/CusC to form the CusCBA periplasm membrane transporter. CusB is an adaptor protein that connects the two membrane proteins CusA (inner membrane) and CusC (outer membrane)...
June 20, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28636368/probing-the-mechanism-of-lal-32-a-gold-nanoparticle-based-antibiotic-discovered-through-small-molecule-variable-ligand-display
#5
Rose Byrne-Nash, Danielle Lucero, Niki Osbaugh, Roberta J Melander, Christian Melander, Daniel L Feldheim
The unrelenting rise of antimicrobial resistant bacteria has necessitated the search for novel antibiotic solutions. Herein we describe further mechanistic studies on a 2.0 nm diameter gold nanoparticle-based antibiotic (designated LAL-32). This antibiotic exhibits bactericidal activity against the Gram-negative bacterium Escherichia coli at 1.0 μΜ, a concentration significantly lower than several clinically available antibiotics (such as ampicillin and gentamicin), and acute treatment with LAL-32 does not give rise to spontaneous resistant mutants...
June 21, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28636010/engineering-multi-functional-bacterial-outer-membrane-vesicles-as-modular-nanodevices-for-biosensing-and-bioimaging
#6
Qi Chen, Sharon Rozovsky, Wilfred Chen
Outer membrane vesicles (OMVs) are proteoliposomes derived from the outer membrane and periplasmic space of many Gram-negative bacteria including E. coli as part of their natural growth cycle. Inspired by the natural ability of E. coli to sort proteins to both the exterior and interior of OMVs, we reported here a one-pot synthesis approach to engineer multi-functionalized OMV-based sensors for both antigen binding and signal generation. SlyB, a native lipoprotein, was used a fusion partner to package nanoluciferase (Nluc) within OMVs, while a previously developed INP-Scaf3 surface scaffold was fused to the Z-domain for antibody recruiting...
June 21, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28634234/the-thioreduction-component-ccmg-confers-efficiency-and-the-heme-ligation-component-ccmh-ensures-stereo-specificity-during-cytochrome-c-maturation
#7
Andreia F Verisssimo, Bahia Khalfaoui-Hassani, Josephine Hwang, Stefan Steimle, Nur Selamoglu, Carsten Sanders, Camilo Khatchikian, Fevzi Daldal
In many Gram-negative bacteria, including Rhodobacter capsulatus, cytochrome c maturation (Ccm) is carried out by a membrane-integral machinery composed of nine proteins (CcmA to I). During this process, the periplasmic thiol-disulfide oxidoreductase DsbA is thought to catalyze the formation of a disulfide bond between the Cys residues at the apocytochrome c heme binding site (CXXCH). Subsequently, a Ccm-specific thioreductive pathway involving CcmG and CcmH reduces this disulfide bond to allow covalent heme ligation...
June 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28632332/microscale-disruption-of-microorganisms-for-parallelized-process-development
#8
REVIEW
Cornelia Walther, Astrid Dürauer
Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris are the standard platforms for biopharmaceutical production with 40% of all between 2010 to 2014 approved protein drugs produced in those microbial hosts. Typically, products overexpressed E. coli and S. cerevisiae remain in the cytosol or are secreted into the periplasm. Consequently, efficient cell disruption is essential for high product recovery during microbial production. Process development platforms at microscale are essential to shorten time to market...
June 20, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28630303/how-members-of-the-human-gut-microbiota-overcome-the-sulfation-problem-posed-by-glycosaminoglycans
#9
Alan Cartmell, Elisabeth C Lowe, Arnaud Baslé, Susan J Firbank, Didier A Ndeh, Heath Murray, Nicolas Terrapon, Vincent Lombard, Bernard Henrissat, Jeremy E Turnbull, Mirjam Czjzek, Harry J Gilbert, David N Bolam
The human microbiota, which plays an important role in health and disease, uses complex carbohydrates as a major source of nutrients. Utilization hierarchy indicates that the host glycosaminoglycans heparin (Hep) and heparan sulfate (HS) are high-priority carbohydrates for Bacteroides thetaiotaomicron, a prominent member of the human microbiota. The sulfation patterns of these glycosaminoglycans are highly variable, which presents a significant enzymatic challenge to the polysaccharide lyases and sulfatases that mediate degradation...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28628094/a-water-soluble-dsbb-variant-that-catalyzes-disulfide-bond-formation-in-vivo
#10
Dario Mizrachi, Michael-Paul Robinson, Guoping Ren, Na Ke, Mehmet Berkmen, Matthew P DeLisa
Escherichia coli DsbB is a transmembrane enzyme that catalyzes the reoxidation of the periplasmic oxidase DsbA by ubiquinone. Here, we sought to convert membrane-bound DsbB into a water-soluble biocatalyst by leveraging a previously described method for in vivo solubilization of integral membrane proteins (IMPs). When solubilized DsbB variants were coexpressed with an export-defective copy of DsbA in the cytoplasm of wild-type E. coli cells, artificial oxidation pathways were created that efficiently catalyzed de novo disulfide-bond formation in a range of substrate proteins, in a manner dependent on both DsbA and quinone...
June 19, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28625985/periplasmic-nicotine-dehydrogenase-ndhab-utilizes-pseudoazurin-as-its-physiological-electron-acceptor-in-agrobacterium-tumefaciens-s33
#11
Wenjun Yu, Rongshui Wang, Haiyan Huang, Huijun Xie, Shuning Wang
Agrobacterium tumefaciens S33 can grow with nicotine as the sole source of carbon, nitrogen, and energy via a novel hybrid of the pyridine pathway and the pyrollidine pathway. Characterization of the enzymes involved in the hybrid pathway is important for understanding its biochemical mechanism. Here we report that the molybdenum-containing nicotine dehydrogenase (NdhAB), which catalyzes the initial step of nicotine degradation, is located in the periplasm of strain S33, while the 6-hydroxynicotine oxidase and 6-hydroxypseudooxynicoine oxidase are in the cytoplasm...
June 16, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28619018/efficient-soluble-expression-of-disulfide-bonded-proteins-in-the-cytoplasm-of-escherichia-coli-in-fed-batch-fermentations-on-chemically-defined-minimal-media
#12
Anna Gąciarz, Narendar Kumar Khatri, M Lourdes Velez-Suberbie, Mirva J Saaranen, Yuko Uchida, Eli Keshavarz-Moore, Lloyd W Ruddock
BACKGROUND: The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in the cytoplasm in the form of inclusion bodies, then solubilized and re-folded in vitro. Both of these approaches have limitations. Previously we showed that soluble expression of disulfide bonded proteins in the cytoplasm of E. coli is possible at shake flask scale with a system, known as CyDisCo, which is based on co-expression of a protein of interest along with a sulfhydryl oxidase and a disulfide bond isomerase...
June 15, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28616083/outer-membrane-phospholipase-a-s-roles-in-helicobacter-pylori-acid-adaptation
#13
Hilde S Vollan, Tone Tannæs, Dominique A Caugant, Gert Vriend, Geir Bukholm
BACKGROUND: The pH of the human gastric mucosa varies around 2.5 so that only bacteria with strong acidic stress tolerance can colonize it. The ulcer causing Helicobacter pylori thrives in the gastric mucosa. We analyse the roles of the key outer membrane protein OMPLA in its roles in acid tolerance. RESULTS: The homology model of Helicobacter pylori outer membrane phospholipase A (OMPLA) reveals a twelve stranded β-barrel with a pore that allows molecules to pass with a diameter up to 4 Å...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28612728/-bdellovibrio-and-like-organisms-outstanding-predators
#14
Édouard Jurkevitch, Stéphan Jacquet
Obligate predatory bacteria, i.e. bacteria requiring a Gram negative prey cell in order to complete their cell cycle, belong to the polyphyletic group referred to as the Bdellovibrio And Like Organisms (BALO). Predatory interactions between bacteria are complex, yet their dynamics and impact on bacterial communities in the environment are becoming better understood. BALO have unique life cycles: they grow epibiotically with the predator remaining attached to the prey's envelope, dividing in a binary manner or periplasmically, i...
May 2017: Médecine Sciences: M/S
https://www.readbyqxmd.com/read/28607072/adaptor-protein-mediates-dynamic-pump-assembly-for-bacterial-metal-efflux
#15
Ace George Santiago, Tai-Yen Chen, Lauren A Genova, Won Jung, Alayna M George Thompson, Megan M McEvoy, Peng Chen
Multicomponent efflux complexes constitute a primary mechanism for Gram-negative bacteria to expel toxic molecules for survival. As these complexes traverse the periplasm and link inner and outer membranes, it remains unclear how they operate efficiently without compromising periplasmic plasticity. Combining single-molecule superresolution imaging and genetic engineering, we study in living Escherichia coli cells the tripartite efflux complex CusCBA of the resistance-nodulation-division family that is essential for bacterial resistance to drugs and toxic metals...
June 12, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28601973/attack-phase-bdellovibrio-bacteriovorus-responses-to-extracellular-nutrients-are-analogous-to-those-seen-during-late-intraperiplasmic-growth
#16
Mohammed Dwidar, Hansol Im, Jeong Kon Seo, Robert J Mitchell
Bdellovibrio bacteriovorus is a predatory bacterium which lives by invading the periplasm of gram-negative bacteria and consuming them from within. This predator was thought to be dependent upon prey for nutrients since it lacks genes encoding for critical enzymes involved in amino acid biosynthesis. This study, however, found that planktonic attack-phase predators are not just dependent upon prey for nutrients, but rather, they respond to nutrients in the surrounding medium and, subsequently, synthesize and secrete proteases in a nutrient-dependent manner...
June 10, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28594235/live-attenuated-auxotrophic-mutant-of-salmonella-typhimurium-expressing-immunogenic-ha1-protein-enhances-immunity-and-protective-efficacy-against-h1n1-influenza-virus-infection
#17
Nitin Machindra Kamble, Kim Je Hyoung, John Hwa Lee
AIM: To evaluate the efficacy of attenuated Salmonella typhimurium (JOL912) as a live bacterial vaccine vector. MATERIALS & METHODS: The JOL912 engineered to deliver HA1 protein from influenza A/Puerto Rico/8/1934 (H1N1; PR8) virus was coined as JOL1635 and further evaluated for immunogenicity and protective efficacy. RESULTS: The JOL1635 stably harbored the HA1 gene within pMMP65 plasmid with periplasmic expression and effective delivery of HA1 protein to RAW264...
June 8, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28593831/osmoregulated-periplasmic-glucans
#18
Sébastien Bontemps-Gallo, Jean-Pierre Bohin, Jean-Marie Lacroix
Among all the systems developed by enterobacteria to face osmotic stress, only osmoregulated periplasmic glucans (OPGs) were found to be modulated during osmotic fluxes. First detected in 1973 by E.P. Kennedy's group in a study of phospholipid turnover in Escherichia coli, OPGs have been shown across alpha, beta, and gamma subdivisions of the proteobacteria. Discovery of OPG-like compounds in the epsilon subdivision strongly suggested that the presence of periplasmic glucans is essential for almost all proteobacteria...
June 2017: EcoSal Plus
https://www.readbyqxmd.com/read/28593349/transcriptomic-analysis-of-the-swarm-motility-phenotype-of-salmonella-enterica-serovar-typhimurium-mutant-defective-in-periplasmic-glucan-synthesis
#19
Arvind A Bhagwat, Lynn Young, Allen D Smith, Medha Bhagwat
Movement of food-borne pathogens on moist surfaces enables them to migrate towards more favorable niches and facilitate their survival for extended periods of time. Salmonella enterica serovar Typhimurium mutants defective in Osmoregulated periplasmic glucans (OPG) synthesis are unable to exhibit motility on moist surfaces (swarming); however, their mobility in liquid (swim motility) remains unaffected. In order to understand the role of OPG in swarm motility, transcriptomic analysis was performed using cells growing on a moist agar surface...
June 8, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28589218/h-n-n-c-%C3%AE-and-c-%C3%AE-assignments-of-the-two-periplasmic-domains-of-neisseria-meningitidis-dsbd
#20
Roxanne P Smith, Biswaranjan Mohanty, Martin L Williams, Martin J Scanlon, Begoña Heras
DsbD is a disulfide bond reductase present in the inner membrane of many Gamma-Proteobacteria. In the human pathogen Neisseria meningitidis, DsbD is required for viability and represents a potential target for the development of antibiotics. Here we report the chemical shift assignments (H(N), N, C(α) and C(β)) for the reduced and oxidized forms of the two periplasmic domains of N. meningitidis DsbD, n-NmDsbD and c-NmDsbD. The backbone amide resonances in all four forms were completely assigned, and the secondary structures for the core regions of the proteins were calculated using (13)C(αβ) shifts...
June 6, 2017: Biomolecular NMR Assignments
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