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https://www.readbyqxmd.com/read/28434914/full-length-oligomeric-structure-of-wzz-determined-by-cryoelectron-microscopy-reveals-insights-into-membrane-bound-states
#1
Richard F Collins, Vasileios Kargas, Brad R Clarke, C Alistair Siebert, Daniel K Clare, Peter J Bond, Chris Whitfield, Robert C Ford
Wzz is an integral inner membrane protein involved in regulating the length of lipopolysaccharide O-antigen glycans and essential for the virulence of many Gram-negative pathogens. In all Wzz homologs, the large periplasmic domain is proposed to be anchored by two transmembrane helices, but no information is available for the transmembrane and cytosolic domains. Here we have studied purified oligomeric Wzz complexes using cryoelectron microscopy and resolved the transmembrane regions within a semi-continuous detergent micelle...
April 11, 2017: Structure
https://www.readbyqxmd.com/read/28432382/two-distinct-periplasmic-enzymes-are-responsible-for-tellurite-tellurate-and-selenite-reduction-by-strain-er-te-48-associated-with-the-deep-sea-hydrothermal-vent-tube-worms-at-the-juan-de-fuca-ridge-black-smokers
#2
Chris Maltman, Lynda J Donald, Vladimir Yurkov
Strain ER-Te-48 isolated from a deep-ocean hydrothermal vent tube worm is capable of resisting and reducing extremely high levels of tellurite, tellurate, and selenite, which are used for respiration anaerobically. Tellurite and tellurate reduction is accomplished by a periplasmic enzyme of 215 kDa comprised of 3 subunits (74, 42, and 25 kDa) in a 2:1:1 ratio. The optimum pH and temperature for activity is 8.0 and 35 °C, respectively. Tellurite reduction has a V max of 5.6 µmol/min/mg protein and a K m of 3...
April 21, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28432347/identification-and-characterization-of-differentially-regulated-type-ivb-pilin-genes-necessary-for-predation-in-obligate-bacterial-predators
#3
Ofir Avidan, Margarita Petrenko, René Becker, Sebastian Beck, Michael Linscheid, Shmuel Pietrokovski, Edouard Jurkevitch
Bdellovibrio bacteriovorus is an obligate predator of bacteria that grows and divides within the periplasm of its prey. Functions involved in the early steps of predation have been identified and characterized, but mediators of prey invasion are still poorly detailed. By combining omics data available for Bdellovibrio and like organisms (BALO's), we identified 43 genes expressed in B. bacteriovorus during the early interaction with prey. These included genes in a tight adherence (TAD) operon encoding for two type IVb fimbriae-like pilin proteins (flp1 and flp2), and their processing and export machinery...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28431927/membrane-insertion-of-f0-c-subunit-of-f0f1-atpase-depends-on-glycolipozyme-mpiase-and-is-stimulated-by-yidc
#4
Hanako Nishikawa, Masaru Sasaki, Ken-Ichi Nishiyama
The F0 c subunit of F0F1 ATPase (F0-c) possesses two membrane-spanning stretches with N- and C-termini exposed to the periplasmic (extracellular) side of the cytoplasmic membrane of E. coli. Although F0-c insertion has been extensively analyzed in vitro by means of protease protection assaying, it is unclear whether such assays allow elucidation of the insertion process faithfully, since the membrane-protected fragment, an index of membrane insertion, is a full-length polypeptide of F0-c, which is the same as the protease-resistant conformation without membrane insertion...
April 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28428543/microspectrofluorimetry-to-dissect-the-permeation-of-ceftazidime-in-gram-negative-bacteria
#5
Anas Allam, Laure Maigre, Julia Vergalli, Estelle Dumont, Bertrand Cinquin, Rodolphe Alves de Sousa, Jelena Pajovic, Elizabeth Pinet, Nikaia Smith, Jean-Philippe Herbeuval, Matthieu Réfrégiers, Isabelle Artaud, Jean-Marie Pagès
A main challenge in chemotherapy is to determine the in cellulo parameters modulating the drug concentration required for therapeutic action. It is absolutely urgent to understand membrane permeation and intracellular concentration of antibiotics in clinical isolates: passing the membrane barrier to reach the threshold concentration inside the bacterial periplasm or cytoplasm is the pivotal step of antibacterial activity. Ceftazidime (CAZ) is a key molecule of the combination therapy for treating resistant bacteria...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28421249/co-effects-of-pyrene-and-nitrate-on-the-activity-and-abundance-of-soil-denitrifiers-under-anaerobic-condition
#6
Zhi-Feng Zhou, Yan-Hong Yao, Ming-Xia Wang, Xiao-Hu Zuo
It has previously been confirmed that polycyclic aromatic hydrocarbons (PAHs) could be degraded by soil microbes coupling with denitrification, but the relationships among soil denitrifiers, PAHs, and nitrate under obligate anaerobic condition are still unclear. Here, co-effects of pyrene and nitrate on the activity and abundance of soil denitrifiers were investigated through a 45-day incubation experiment. Two groups of soil treatments with (N30) and without (N0) nitrate (30 mg kg(-1) dry soil) amendment were conducted, and each group contained three treatments with different pyrene concentrations (0, 30, and 60 mg kg(-1) dry soil denoted as P0, P30, and P60, respectively)...
April 18, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28419595/lipopolysaccharide-lps-binding-to-the-periplasmic-protein-lpta
#7
Kathryn M Schultz, Tanner J Lundquist, Candice S Klug
Lipopolysaccharide (LPS) and the periplasmic protein LptA are two essential components of Gram-negative bacteria. LPS, also known as endotoxin, is found asymmetrically distributed in the outer leaflet of the outer membrane of Gram-negative bacteria such as Escherichia coli and plays a role in the organism's natural defenses in adverse environmental conditions. LptA is a member of the lipopolysaccharide transport protein (Lpt) family, which also includes LptC, LptDE, and LptBFG2 , that functions to transport LPS through the periplasm to the outer leaflet of the outer membrane after MsbA flips LPS across the inner membrane...
April 17, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28417370/determining-the-localization-of-carbohydrate-active-enzymes-within-gram-negative-bacteria
#8
Richard McLean, G Douglas Inglis, Steven C Mosimann, Richard R E Uwiera, D Wade Abbott
Investigating the subcellular location of secreted proteins is valuable for illuminating their biological function. Although several bioinformatics programs currently exist to predict the destination of a trafficked protein using its signal peptide sequence, these programs have limited accuracy and often require experimental validation. Here, we present a systematic method to fractionate gram-negative cells and characterize the subcellular localization of secreted carbohydrate active enzymes (CAZymes). This method involves four parallel approaches that reveal the relative abundance of protein within the cytoplasm, periplasm, outer membrane, and extracellular environment...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28416709/spheres-of-hope-packets-of-doom-the-good-and-bad-of-outer-membrane-vesicles-in-interspecies-and-ecological-dynamics
#9
Jonathan B Lynch, Rosanna A Alegado
Outer membrane vesicles (OMVs) are proteoliposome nanoparticles ubiquitously produced by Gram-negative bacteria. Typically bearing similar composition to the outer membrane and periplasm of the cells from which they are derived, OMVs package an array of proteins, lipids, and nucleic acids. Once considered inconsequential byproducts of bacterial growth, OMVs have since been demonstrated to mediate cellular stress relief, promote horizontal gene transfer and antimicrobial activity, and elicit metazoan inflammation...
April 17, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28416660/redefining-the-essential-trafficking-pathway-for-outer-membrane-lipoproteins
#10
Marcin Grabowicz, Thomas J Silhavy
The outer membrane (OM) of Gram-negative bacteria is a permeability barrier and an intrinsic antibiotic resistance factor. Lipoproteins are OM components that function in cell wall synthesis, diverse secretion systems, and antibiotic efflux pumps. Moreover, each of the essential OM machines that assemble the barrier requires one or more lipoproteins. This dependence is thought to explain the essentiality of the periplasmic chaperone LolA and its OM receptor LolB that traffic lipoproteins to the OM. However, we show that in strains lacking substrates that are toxic when mislocalized, both LolA and LolB can be completely bypassed by activating an envelope stress response without compromising trafficking of essential lipoproteins...
April 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28413748/thiamine-assays-advances-challenges-and-caveats
#11
REVIEW
Katie A Edwards, Nicole Tu-Maung, Krystal Cheng, Binbin Wang, Antje J Baeumner, Clifford E Kraft
Thiamine (vitamin B1) is essential to the health of all living organisms and deficiency has long been associated with diseases in animals such as fish, birds, alligators, and domesticated ruminant mammals. Thiamine is also implicated in several human diseases including Alzheimer's, diabetes, dementia, depression and, most notably, Wernicke-Korsakoff syndrome and Beriberi disease. Yet, highly sensitive and specific detection of thiamine remains an analytical challenge, as pM to nm levels of thiamine need to be detected in environmental and human samples, respectively, various phosphorylated variants need to be discriminated, and rapid on-site detection would be highly desirable...
April 2017: ChemistryOpen
https://www.readbyqxmd.com/read/28412337/an-overview-on-molecular-chaperones-enhancing-solubility-of-expressed-recombinant-proteins-with-correct-folding
#12
REVIEW
Mina Mamipour, Mohammadreza Yousefi, Mohammad Hasanzadeh
The majority of research topics declared that most of the recombinant proteins have been expressed by Escherichia coli in basic investigations. But the majority of high expressed proteins formed as inactive recombinant proteins that are called inclusion body. To overcome this problem, several methods have been used including suitable promoter, environmental factors, ladder tag to secretion of proteins into the periplasm, gene protein optimization, chemical chaperones and molecular chaperones sets. Co-expression of the interest protein with molecular chaperones is one of the common methods The chaperones are a group of proteins, which are involved in making correct folding of recombinant proteins...
April 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28408605/nanoscale-length-control-of-the-flagellar-driveshaft-requires-hitting-the-tethered-outer-membrane
#13
Eli J Cohen, Josie L Ferreira, Mark S Ladinsky, Morgan Beeby, Kelly T Hughes
The bacterial flagellum exemplifies a system where even small deviations from the highly regulated flagellar assembly process can abolish motility and cause negative physiological outcomes. Consequently, bacteria have evolved elegant and robust regulatory mechanisms to ensure that flagellar morphogenesis follows a defined path, with each component self-assembling to predetermined dimensions. The flagellar rod acts as a driveshaft to transmit torque from the cytoplasmic rotor to the external filament. The rod self-assembles to a defined length of ~25 nanometers...
April 14, 2017: Science
https://www.readbyqxmd.com/read/28407382/carbohydrate-uptake-in-advenella-mimigardefordensis-strain-dpn7t-is-mediated-by-periplasmic-sugar-oxidation-and-a-trap-transport-system
#14
Christina Meinert, Jana Senger, Marco Witthohn, Jan Hendrik Wübbeler, Alexander Steinbüchel
In this study, we investigated an SBP (DctPAm) of a tripartite ATP-independent periplasmic transport system (TRAP) in Advenella mimigardefordensis strain DPN7(T) . Deletion of dctPAm as well as of the two transmembrane compounds of the tripartite transporter, dctQ and dctM, impaired growth of A. mimigardefordensis strain DPN7(T) , if cultivated on mineral salt medium supplemented with D-glucose, D-galactose, L-arabinose, D-fucose, D-xylose or D-gluconic acid, respectively. The wild type phenotype was restored during complementation studies of A...
April 13, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28407314/on-the-pull-periplasmic-trapping-of-sugars-before-transport
#15
Gavin Thomas
No abstract text is available yet for this article.
April 13, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28405872/a-novel-method-for-assessment-of-local-ph-in-periplasmic-space-and-of-cell-surface-potential-in-yeast
#16
Jaromír Plášek, David Babuka, Dana Gášková, Iva Jančíková, Jakub Zahumenský, Milan Hoefer
Yeast cells exhibit a negative surface potential due to negative charges at the cell membrane surface. Consequently, local concentrations of cations at the periplasmic membrane surface may be significantly increased compared to their bulk environment. However, in cell suspensions only bulk concentrations of cations can be measured directly. Here we present a novel method enabling the assessment of local pH at the periplasmic membrane surface which can be directly related to the underlying cell surface potential...
April 12, 2017: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/28401513/study-of-intra-inter-species-protein-protein-interactions-for-potential-drug-targets-identification-and-subsequent-drug-design-for-escherichia-coli-o104-h4-c277-11
#17
Shakhinur Islam Mondal, Zabed Mahmud, Montasir Elahi, Arzuba Akter, Nurnabi Azad Jewel, Md Muzahidul Islam, Sabiha Ferdous, Taisei Kikuchi
Protein-protein interaction (PPI) and host-pathogen interactions (HPI) proteomic analysis has been successfully practiced for potential drug target identification in pathogenic infections. In this research, we attempted to identify new drug target based on PPI and HPI computation approaches and subsequently design new drug against devastating enterohemorrhagic Escherichia coli O104:H4 C277-11 (Broad), which causes life-threatening food borne disease outbreak in Germany and other countries in Europe in 2011...
December 2016: In Silico Pharmacology
https://www.readbyqxmd.com/read/28400767/the-periplasmic-chaperone-network-of-campylobacter-jejuni-evidence-that-salc-cj1289-and-ppid-cj0694-are-involved-in-maintaining-outer-membrane-integrity
#18
Aidan J Taylor, Shadi A I Zakai, David J Kelly
The outer membrane (OM) of Gram-negative pathogenic bacteria is a key structure in host-pathogen interactions that contains a plethora of proteins, performing a range of functions including adhesion, nutrient uptake, export of effectors and interaction with innate and adaptive components of the immune system. In addition, the OM can exclude drugs and thus contribute to antimicrobial resistance. The OM of the food-borne pathogen Campylobacter jejuni contains porins, adhesins and other virulence factors that must be specifically localized to this membrane, but the protein sorting mechanisms involved are only partially understood...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28397983/high-yield-production-of-recombinant-crm197-a-non-toxic-mutant-of-diphtheria-toxin-in-the-periplasm-of-escherichia-coli
#19
Philippe Goffina, Marianne Dewerchin, Philippe De Rop, Normand Blais, Philippe Dehottay
A high cell density fed-batch process was developed for production of recombinant CRM197, a non-toxic mutant of diphtheria toxin widely used as a carrier in polysaccharide-protein conjugate vaccines. Fully soluble recombinant CRM197 was obtained in high yields and with an authentic N-terminus, by targeting the protein to the periplasm of Escherichia coli using the Signal Recognition Particle (SRP)-dependent signal sequence of FlgI. Response Surface Methodology was used to optimize the set-points of key process parameters (pH and feed rate at induction)...
April 11, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28397401/anaerobic-utilization-of-fe-iii-xenosiderophores-among-bacteroides-species-and-the-distinct-assimilation-of-fe-iii-ferrichrome-by-bacteroides-fragilis-within-the-genus
#20
Edson R Rocha, Anna S Krykunivsky
In this study, we show that Bacteroides species utilize Fe(III)-xenosiderophores as the only source of exogenous iron to support growth under iron-limiting conditions in vitro anaerobically. Bacteroides fragilis was the only species able to utilize Fe(III)-ferrichrome while Bacteroides vulgatus ATCC 8482 and Bacteroides thetaiotaomicron VPI 5482 were able to utilize both Fe(III)-enterobactin and Fe(III)-salmochelin S4 as the only source of iron in a dose-dependent manner. We have investigated the way B. fragilis assimilates Fe(III)-ferrichrome as initial model to understand the utilization of xenosiderophores in anaerobes...
April 11, 2017: MicrobiologyOpen
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