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https://www.readbyqxmd.com/read/29232112/switchable-control-of-antibiotic-activity-a-shape-shifting-tail-strategy
#1
Jinming Chang, Yi Chen, Zhou Xu, Zhonghui Wang, Qi Zeng, Haojun Fan
Bacterial resistance is emerging as a global threat, stemming partially from continuous exposure of pathogens to antibiotics of sublethal concentrations. Thus, novel molecular approaches capable of inactivating antibiotics, which prevent their final build-up in the environment, are highly desirable. Here, we report a proof-of-principle demonstration of a mechanically new strategy for switchable control of antibiotic activity, which regulates drug uptake across the outer membrane of Gram-negative bacteria by externally triggered shape shifting of a short, covalently attached "tail"...
December 12, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29230915/periapical-infection-may-affect-birth-outcomes-via-systemic-inflammation
#2
U Harjunmaa, R Doyle, J Järnstedt, S Kamiza, J M Jorgensen, C P Stewart, L Shaw, L Hallamaa, U Ashorn, N Klein, K G Dewey, K Maleta, P Ashorn
OBJECTIVES: Maternal dental periapical infections are associated with preterm birth and intrauterine growth restriction. This study investigates if the association is mediated through bacterial spread from periapical lesions to placenta (direct pathway) or systemic inflammatory reaction (indirect pathway). MATERIALS AND METHODS: We compared birth outcomes in Malawian mothers with and without periapical infection. As markers of a direct pathway, we identified placental bacteria using a 16S rDNA approach, and assessed histological evidence of inflammation in the placenta and amniotic membranes...
December 12, 2017: Oral Diseases
https://www.readbyqxmd.com/read/29230232/chemosensitization-of-fusarium-graminearum-to-chemical-fungicides-using-cyclic-lipopeptides-produced-by-bacillus-amyloliquefaciens-strain-jck-12
#3
Kihyun Kim, Yoonji Lee, Areum Ha, Ji-In Kim, Ae Ran Park, Nan Hee Yu, Hokyoung Son, Gyung Ja Choi, Hae Woong Park, Chul Won Lee, Theresa Lee, Yin-Won Lee, Jin-Cheol Kim
Fusarium head blight (FHB) caused by infection with Fusarium graminearum leads to enormous losses to crop growers, and may contaminate grains with a number of Fusarium mycotoxins that pose serious risks to human and animal health. Antagonistic bacteria that are used to prevent FHB offer attractive alternatives or supplements to synthetic fungicides for controlling FHB without the negative effects of chemical management. Out of 500 bacterial strains isolated from soil, Bacillus amyloliquefaciens JCK-12 showed strong antifungal activity and was considered a potential source for control strategies to reduce FHB...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29229778/structural-and-functional-insights-into-the-role-of-bamd-and-bame-within-the-%C3%AE-barrel-assembly-machinery-in-neisseria-gonorrhoeae
#4
Aleksandra E Sikora, Igor H Wierzbicki, Ryszard A Zielke, Rachael F Ryner, Konstantin V Korotkov, Susan K Buchanan, Nicholas Noinaj
The β-barrel assembly machinery (BAM) is a conserved multi-component protein complex responsible for the biogenesis of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. Given its role in the production of OMPs for survival and pathogenesis, BAM represents an attractive target for the development of therapeutic interventions including drugs and vaccines against multi-drug resistant bacteria such as Neisseria gonorrhoeae The first structure of BamA, the central component of BAM, was from N...
December 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29229504/an-inhibitory-action-of-chitosan-nanoparticles-against-pathogenic-bacteria-and-fungi-and-their-potential-applications-as-biocompatible-antioxidants
#5
D MubarakAli, F LewisOscar, V Gopinath, Naify S Alharbi, Sulaiman Ali Alharbi, N Thajuddin
Chitosan is the second most abundant polymer obtained from the byproduct of seafood. Chitosan and its derivatives and chitosan loaded drugs are the recent area of interest against microbial pathogenesis. The cationic chitosan nanoparticles (ChNPs) interact with the anionic surfaces of the microbial cell membrane, which promotes antimicrobial activity. Although, ChNPs are potential against pathogenic microbes, selection of adaptable, suitable and cost effective synthesis method is much important. In the present study, ChNPs were synthesized adopting ionic gelation using sodium tripolyphosphate as a cross linking agent and characterized by FTIR, DLS, SEM and TEM analysis...
December 8, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29227933/synthesis-biological-evaluation-and-metabolic-stability-of-phenazine-derivatives-as-antibacterial-agents
#6
Maddeboina Krishnaiah, Nathalia Rodrigues de Almeida, Venkatareddy Udumula, Zhongcheng Song, Yashpal Singh Chhonker, Mai M Abdelmoaty, Valter Aragao do Nascimento, Daryl J Murry, Martin Conda-Sheridan
Drug-resistant pathogens are a major cause of hospital- and community-associated bacterial infections in the United States and around the world. These infections are increasingly difficult to treat due to the development of antibiotic resistance and the formation of bacterial biofilms. In the paper, a series of phenazines were synthesized and evaluated for their in vitro antimicrobial activity against Gram positive (methicillin resistant staphylococcus aureus, MRSA) and Gram negative (Escherichia coli, E. coli) bacteria...
November 9, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29227931/synthesis-and-antibacterial-evaluation-of-novel-cationic-chalcone-derivatives-possessing-broad-spectrum-antibacterial-activity
#7
Wen-Chao Chu, Peng-Yan Bai, Zhao-Qing Yang, De-Yun Cui, Yong-Gang Hua, Yi Yang, Qian-Qian Yang, En Zhang, Shangshang Qin
There is an urgent need to identify new antibiotics with novel mechanisms that combat antibiotic resistant bacteria. Herein, a series of chalcone derivatives that mimic the essential properties of cationic antimicrobial peptides were designed and synthesized. Antibacterial activities against drug-sensitive bacteria, including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Salmonella enterica, as well as clinical multiple drug resistant isolates of methicillin-resistant S. aureus (MRSA), KPC-2-producing and NDM-1-producing Carbapenem-resistant Enterobacteriaceae were evaluated...
December 5, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29222414/structural-dynamics-of-the-mscl-c-terminal-domain
#8
Navid Bavi, Adam D Martinac, D Marien Cortes, Omid Bavi, Pietro Ridone, Takeshi Nomura, Adam P Hill, Boris Martinac, Eduardo Perozo
The large conductance mechanosensitive channel (MscL), acts as an osmoprotective emergency valve in bacteria by opening a large, water-filled pore in response to changes in membrane tension. In its closed configuration, the last 36 residues at the C-terminus form a bundle of five α-helices co-linear with the five-fold axis of symmetry. Here, we examined the structural dynamics of the C-terminus of EcMscL using site-directed spin labelling electron paramagnetic resonance (SDSL EPR) spectroscopy. These experiments were complemented with computational modelling including molecular dynamics (MD) simulations and finite element (FE) modelling...
December 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29222343/bacterial-cyclic-%C3%AE-1-2-glucans-sequester-iron-to-protect-against-iron-induced-toxicity
#9
Sreegowrinadh Javvadi, Sheo Shankar Pandey, Amita Mishra, Binod Bihari Pradhan, Subhadeep Chatterjee
Cellular iron homeostasis is critical for survival and growth. Bacteria employ a variety of strategies to sequester iron from the environment and to store intracellular iron surplus that can be utilized in iron-restricted conditions while also limiting the potential for the production of iron-induced reactive oxygen species (ROS). Here, we report that membrane-derived oligosaccharide (mdo) glucan, an intrinsic component of Gram-negative bacteria, sequesters the ferrous form of iron. Iron-binding, uptake, and localization experiments indicated that both secreted and periplasmic β-(1,2)-glucans bind iron specifically and promote growth under iron-restricted conditions...
December 8, 2017: EMBO Reports
https://www.readbyqxmd.com/read/29221882/effective-inhibition-of-cbf-14-against-cryptococcus-neoformans-infection-in-mice-and-its-related-anti-inflammatory-activity
#10
Changzhong Yu, Shanshan Wei, Xiaorong Han, Hanhan Liu, Mengxiao Wang, Meiling Jiang, Min Guo, Jie Dou, Changlin Zhou, Lingman Ma
Cbf-14 (RLLRKFFRKLKKSV), a designed peptide derived from cathelicidin family AMP, has proven to be potent against drug-resistant bacteria. In the present study, we investigated the anti-cryptococcal activity of Cbf-14 in vitro and in a pulmonary infection mouse model. Sensitivity test indicated that Cbf-14 possessed effective antifungal activity against Cryptococcus neoformans with an MIC of 4-16 µg/ml, and killing experiments showed that fungicidal activity was achieved after only 4 h treatment with Cbf-14 at 4× MIC concentrations in vitro...
December 5, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29219809/the-cell-wall-of-the-filamentous-anoxygenic-phototrophic-bacterium-oscillochloris-trichoides
#11
Olga I Keppen, Ruslan N Ivanovsky, Galina M Streshinskaya, Natalia V Lebedeva, Alexander S Shashkov, Andrey S Dmitrenok, Denis S Grouzdev
The filamentous anoxygenic phototrophic bacterium Oscillochloris trichoides DG-6 has been studied, and it has been shown that there are no lipopolysaccharides on the cell surface. Fatty acids hydroxylated at the C3 position, amino sugars and phosphate-containing compounds characteristic of lipid A have also not been found. The genes encoding for proteins responsible for the synthesis of lipopolysaccharides and the genes for the transport system, usually localized in the outer membrane of Gram-negative bacteria, have not been detected in the genome...
December 8, 2017: Microbiology
https://www.readbyqxmd.com/read/29218056/respiratory-burst-oxidase-homolog-gene-a-is-crucial-for-rhizobium-infection-and-nodule-maturation-and-function-in-common-bean
#12
Manoj-Kumar Arthikala, Jesús Montiel, Rosana Sánchez-López, Noreide Nava, Luis Cárdenas, Carmen Quinto
Reactive oxygen species (ROS) produced by respiratory burst oxidase homologs (RBOHs) regulate numerous plant cell processes, including the symbiosis between legumes and nitrogen-fixing bacteria. Rapid and transient ROS production was reported after Phaseolus vulgaris root hairs were treated with Nod factors, indicating the presence of a ROS-associated molecular signature in the symbiosis signaling pathway. Rboh is a multigene family containing nine members (RbohA-I) in P. vulgaris. RNA interference of RbohB suppresses ROS production and attenuates rhizobial infection thread (IT) progression in P...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29218041/mrp-antiporters-have-important-roles-in-diverse-bacteria-and-archaea
#13
REVIEW
Masahiro Ito, Masato Morino, Terry A Krulwich
Mrp (Multiple resistance and pH) antiporter was identified as a gene complementing an alkaline-sensitive mutant strain of alkaliphilic Bacillus halodurans C-125 in 1990. At that time, there was no example of a multi-subunit type Na+/H+ antiporter comprising six or seven hydrophobic proteins, and it was newly designated as the monovalent cation: proton antiporter-3 (CPA3) family in the classification of transporters. The Mrp antiporter is broadly distributed among bacteria and archaea, not only in alkaliphiles...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29217618/lps-upregulates-palmitoylated-enzymes-of-the-phosphatidylinositol-cycle-an-insight-from-proteomic-studies
#14
Justyna Sobocińska, Paula Roszczenko-Jasińska, Monika Zaręba-Kozioł, Aneta Hromada-Judycka, Orest V Matveichuk, Gabriela Traczyk, Katarzyna Łukasiuk, Katarzyna Kwiatkowska
Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria which induces strong pro-inflammatory reactions of mammals. These processes are triggered upon sequential binding of LPS to CD14, a GPI-linked plasma membrane raft protein, and to the TLR4/MD2 receptor complex. We have found earlier that upon LPS binding CD14 triggers generation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], a lipid controlling subsequent pro-inflammatory cytokine production. Here we show that stimulation of RAW264 macrophage-like cells with LPS induces global changes of the level of fatty-acylated, most likely palmitoylated, proteins...
December 7, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29216517/go-agcl-ag-nanocomposites-with-enhanced-visible-light-driven-catalytic-properties-for-antibacterial-and-biofilm-disrupting-applications
#15
Xinhuan Wang, Qiusen Han, Ning Yu, Tian Wang, Chen Wang, Rong Yang
Nanomaterials with visible light-driven photocatalytic activity have attracted much attention due to their excellent abilities in degradation of various organic pollutants as well as inactivating bacteria. Herein, graphene oxide (GO) enwrapped silver chloride/silver (AgCl/Ag) nanocomposites with high visible light absorption were designed and fabricated as efficient antibacterial agents. AgCl NPs were synthesized in the presence of GO first and Ag NPs were coated on AgCl surface by heat reduction to form GO-AgCl/Ag nanocomposites...
November 26, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29215320/early-effects-of-rhodomyrtone-on-membrane-integrity-in-methicillin-resistant-staphylococcus-aureus
#16
Wipawadee Sianglum, Dennapa Saeloh, Pongsri Tongtawe, Natthakul Wootipoom, Nitaya Indrawattana, Supayang Piyawan Voravuthikunchai
Strong evidence of high potency of rhodomyrtone as a promising antibacterial agent against pathogenic gram-positive bacteria has been clearly demonstrated in our previous work. The aim of this study was to provide insight into early action of rhodomyrtone, an acylphloroglucinol, on membrane damage in multidrug-resistant methicillin-resistant Staphylococcus aureus (MRSA). Early effects of rhodomyrtone on the bacterial membrane integrity were detected in a time-course study. Flow cytometry revealed a reduction in green fluorescent emission and increase in uptake of propidium iodide in rhodomyrtone-treated bacterial cells in a concentration- and time-dependent manner...
December 7, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29214045/the-rumen-microbiome-an-underexplored-resource-for-novel-antimicrobial-discovery
#17
Linda B Oyama, Susan E Girdwood, Alan R Cookson, Narcis Fernandez-Fuentes, Florence Privé, Hannah E Vallin, Toby J Wilkinson, Peter N Golyshin, Olga V Golyshina, Ralf Mikut, Kai Hilpert, Jennifer Richards, Mandy Wootton, Joan E Edwards, Marc Maresca, Josette Perrier, Fionnuala T Lundy, Yu Luo, Mei Zhou, Matthias Hess, Hilario C Mantovani, Christopher J Creevey, Sharon A Huws
Antimicrobial peptides (AMPs) are promising drug candidates to target multi-drug resistant bacteria. The rumen microbiome presents an underexplored resource for the discovery of novel microbial enzymes and metabolites, including AMPs. Using functional screening and computational approaches, we identified 181 potentially novel AMPs from a rumen bacterial metagenome. Here, we show that three of the selected AMPs (Lynronne-1, Lynronne-2 and Lynronne-3) were effective against numerous bacterial pathogens, including methicillin-resistant Staphylococcus aureus (MRSA)...
2017: NPJ Biofilms and Microbiomes
https://www.readbyqxmd.com/read/29213101/modulation-of-the-neisseria-gonorrhoeae-drug-efflux-conduit-mtre
#18
Giulia Tamburrino, Salomé Llabrés, Owen N Vickery, Samantha J Pitt, Ulrich Zachariae
Widespread antibiotic resistance, especially of Gram-negative bacteria, has become a severe concern for human health. Tripartite efflux pumps are one of the major contributors to resistance in Gram-negative pathogens, by efficiently expelling a broad spectrum of antibiotics from the organism. In Neisseria gonorrhoeae, one of the first bacteria for which pan-resistance has been reported, the most expressed efflux complex is MtrCDE. Here we present the electrophysiological characterisation of the outer membrane component MtrE and the membrane fusion protein MtrC, obtained by a combination of planar lipid bilayer recordings and in silico techniques...
December 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29212086/comparative-analyses-of-transport-proteins-encoded-within-the-genomes-of-bdellovibrio-bacteriovorus-hd100-and-bdellovibrio-exovorus-jss
#19
Fereshteh Heidari Tajabadi, Arturo Medrano-Soto, Masoud Ahmadzadeh, Gholamreza Salehi Jouzani, Milton H Saier
Bdellovibrio, δ-proteobacteria, including B. bacteriovorus (Bba) and B. exovorus (Bex), are obligate predators of other Gram-negative bacteria. While Bba grows in the periplasm of the prey cell, Bex grows externally. We have analyzed and compared the transport proteins of these 2 organisms based on the current contents of the Transporter Classification Database (TCDB; www.tcdb.org). Bba has 103 transporters more than Bex, 50% more secondary carriers, and 3 times as many MFS carriers. Bba has far more metabolite transporters than Bex as expected from its larger genome, but there are 2 times more carbohydrate uptake and drug efflux systems, and 3 times more lipid transporters...
December 7, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29212056/fe3-saturated-montmorillonite-effectively-deactivates-bacteria-in-wastewater
#20
Chao Qin, Chaoqi Chen, Chao Shang, Kang Xia
Existing water disinfection practices often produce harmful disinfection byproducts. The antibacterial activity of Fe3+-saturated montmorillonite was investigated mechanistically using municipal wastewater effluents. Bacterial deactivation efficiency (bacteria viability loss) was 92±0.64% when a secondary wastewater effluent was mixed with Fe3+-saturated montmorillonite for 30min, and further enhanced to 97±0.61% after 4h. This deactivation efficiency was similar to that when the same effluent was UV-disinfected before it exited a wastewater treatment plant...
December 3, 2017: Science of the Total Environment
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