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https://www.readbyqxmd.com/read/29218093/p2x7-receptor-regulates-sympathoexcitatory-response-in-myocardial-infarction-rats-via-nf-%C3%AE%C2%BAb-and-mapk-pathways
#1
Qin Wu, Hongtao Xu, Ling Hao, Guifang Ma, Jinxia Sun, Xianghe Song, Fengyun Ding, Nan Wang
Previous studies have provided evidence for the regulatory effect of P2X7 receptor (P2X7R) on cardiovascular activities. Our study focused on exploring the function and fundamental mechanism of microglial P2X7R in controlling sympathoexcitatory response using rats with acute myocardial infarction (AMI). Coronary artery ligation was used in rats to cause AMI. And before that, rats were administrated with P2X7R siRNA that targeted P2X7R mRNA into paraventricular nucleus (PVN) or BBG (Brilliant Blue G, a P2X7 receptor antagonist)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29216750/pde-camp-epac-c-ebp-%C3%AE-signaling-cascade-regulates-mitochondria-biogenesis-of-tubular-epithelial-cells-in-renal-fibrosis
#2
Hao Ding, Feng Bai, Hongdi Cao, Jing Xu, Li Fang, JiNing Wu, Qi Yuan, Yang Zhou, Qi Sun, WeiChun He, ChunSun Dai, Ke Zen, Lei Jiang, Junwei Yang
AIM: cAMP is a universal second messenger that plays an important role in intracellular signal transduction. cAMP is synthesized by adenylate cyclases (ACs) from adenosine triphosphate (ATP), and terminated by the phosphodiesterases (PDEs). In the present study, we investigated the role of the cAMP pathway in tubular epithelial cells mitochondrial biogenesis in the pathogenesis of renal fibrosis. RESULTS: We found that the cAMP levels were decreased in fibrotic kidney tissues, and replenishing cAMP could ameliorate tubular atrophy and extracellular matrix deposition...
December 7, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/29215320/early-effects-of-rhodomyrtone-on-membrane-integrity-in-methicillin-resistant-staphylococcus-aureus
#3
Wipawadee Sianglum, Dennapa Saeloh, Pongsri Tongtawe, Natthakul Wootipoom, Nitaya Indrawattana, Supayang Piyawan Voravuthikunchai
Strong evidence of high potency of rhodomyrtone as a promising antibacterial agent against pathogenic gram-positive bacteria has been clearly demonstrated in our previous work. The aim of this study was to provide insight into early action of rhodomyrtone, an acylphloroglucinol, on membrane damage in multidrug-resistant methicillin-resistant Staphylococcus aureus (MRSA). Early effects of rhodomyrtone on the bacterial membrane integrity were detected in a time-course study. Flow cytometry revealed a reduction in green fluorescent emission and increase in uptake of propidium iodide in rhodomyrtone-treated bacterial cells in a concentration- and time-dependent manner...
December 7, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29215148/31-p-t2-s-of-phosphomonoesters-phosphodiesters-and-inorganic-phosphate-in-the-human-brain-at-7t
#4
Wybe J M van der Kemp, Dennis W J Klomp, Jannie P Wijnen
PURPOSE: To determine the phosphorus-31 T2 s of phosphomonoesters, phosphodiesters, and inorganic phosphate in the healthy human brain at 7T. METHODS: A 3D chemical shift imaging multi-echo sequence with composite block pulses for refocusing was used to measure one free induction decay (FID) and seven full echoes with an echo spacing of 45 ms on the brain of nine healthy volunteers (age range 22-45 years; average age 27 ± 8 years). Spectral fitting was used to determine the change in metabolic signal amplitude with echo time...
December 7, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29209319/mesenchymal-stem-cells-induce-expression-of-cd73-in-human-monocytes-in-vitro-and-in-a-swine-model-of-myocardial-infarction-in-vivo
#5
Marta Monguió-Tortajada, Santiago Roura, Carolina Gálvez-Montón, Marcella Franquesa, Antoni Bayes-Genis, Francesc E Borràs
The ectoenzymes CD39 and CD73 regulate the purinergic signaling through the hydrolysis of adenosine triphosphate (ATP)/ADP to AMP and to adenosine (Ado), respectively. This shifts the pro-inflammatory milieu induced by extracellular ATP to the anti-inflammatory regulation by Ado. Mesenchymal stem cells (MSCs) have potent immunomodulatory capabilities, including monocyte modulation toward an anti-inflammatory phenotype aiding tissue repair. In vitro, we observed that human cardiac adipose tissue-derived MSCs (cATMSCs) and umbilical cord MSCs similarly polarize monocytes toward a regulatory M2 phenotype, which maintained the expression of CD39 and induced expression of CD73 in a cell contact dependent fashion, correlating with increased functional activity...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29207041/mitochondria-mediated-damage-to-dopaminergic-neurons-in-parkinson-s-disease-review
#6
Xiao-Liang Liu, Ying-Di Wang, Xiu-Ming Yu, Da-Wei Li, Guang-Ren Li
Mitochondria are important organelles in virtually all eukaryotic cells, and are involved in a wide range of physiological and pathophysiological processes. Besides the generation of cellular energy in the form of adenosine triphosphate, mitochondria are also involved in calcium homeostasis, reactive oxygen species production and the activation of the intrinsic cell death pathway, thus determining cell survival and death. Mitochondrial abnormalities have been implicated in a wide range of disorders, including neurodegenerative disease such as Parkinson's disease (PD), and considered as a primary cause and central event responsible for the progressive loss of dopaminergic neurons in PD...
November 16, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29207023/macrophage-migration-inhibitory-factor-promotes-warburg-effect-via-activation-of-the-nf%C3%A2-%C3%AE%C2%BAb-hif%C3%A2-1%C3%AE-pathway-in-lung-cancer
#7
Jun Li, Junhua Zhang, Fengjiao Xie, Jiangzhou Peng, Xu Wu
Macrophage migration inhibitory factor (MIF) is upregulated in various solid tumors, a process that is associated with tumor progression and metastasis. The present study aimed to investigate the role and the underlying mechanism of MIF in human lung cancer. Human lung cancer H358, H460, H524, H1650, H838, H1975 and A549 cell lines were used to examine the expression of MIF by real time‑quantitative polymerase chain reaction and western blotting. The lentivirus was used to overexpress MIF and the expression of MIF and hypoxia‑inducible factor 1‑α (HIF‑1α) were knocked down by shRNA or siRNA...
November 22, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29206177/new-therapeutic-agent-against-arterial-thrombosis-an-iridium-iii-derived-organometallic-compound
#8
Chih-Wei Hsia, Marappan Velusamy, Jeng-Ting Tsao, Chih-Hsuan Hsia, Duen-Suey Chou, Thanasekaran Jayakumar, Lin-Wen Lee, Jiun-Yi Li, Joen-Rong Sheu
Platelet activation plays a major role in cardio and cerebrovascular diseases, and cancer progression. Disruption of platelet activation represents an attractive therapeutic target for reducing the bidirectional cross talk between platelets and tumor cells. Platinum (Pt) compounds have been used for treating cancer. Hence, replacing Pt with iridium (Ir) is considered a potential alternative. We recently developed an Ir(III)-derived complex, [Ir(Cp*)1-(2-pyridyl)-3-(2-hydroxyphenyl)imidazo[1,5-a]pyridine Cl]BF₄ (Ir-11), which exhibited strong antiplatelet activity; hence, we assessed the therapeutic potential of Ir-11 against arterial thrombosis...
December 5, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29202437/converting-pyrophosphate-generated-during-loop-mediated-isothermal-amplification-to-atp-application-to-electrochemical-detection-of-nosema-bombycis-genomic-dna-ptp1
#9
Shunbi Xie, Ying Tang, Dianyong Tang
Traditionally, genomic DNA detection is relay on a rigorous DNA amplification process, which always accompanied with complicated gel electrophoresis or expensive fluorescence detection methods. In this work, we have translated genomic DNA detection into adenosine triphosphate (ATP) test based on a split aptamer-based electrochemical sandwich assay. The key characteristic of our method are list as follows: first, nucleic acid amplification of the target gene was performed by the use of a loop mediated isothermal amplification (LAMP) process...
December 1, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29200326/probenecid-directly-impairs-activation-of-the-canine-p2x7-receptor
#10
Rachael Bartlett, Leanne Stokes, Stephen J Curtis, Belinda L Curtis, Ronald Sluyter
The current study aimed to determine if probenecid could directly impair the canine P2X7 receptor, a ligand-gated cation channel activated by extracellular adenosine 5'-triphosphate (ATP). Patch clamp measurements demonstrated that probenecid impairs ATP-induced inward currents in HEK-293 cells expressing canine P2X7. Flow cytometric measurements of ethidium+ uptake into HEK-293 cells expressing canine P2X7 showed that probenecid impairs ATP-induced pore formation in a concentration-dependent manner, with a half maximal inhibitory concentration of 158 µM...
December 4, 2017: Nucleosides, Nucleotides & Nucleic Acids
https://www.readbyqxmd.com/read/29199560/lion-s-mane-medicinal-mushroom-hericium-erinaceus-agaricomycetes-modulates-purinoceptor-coupled-calcium-signaling-and-murine-nociceptive-behavior
#11
Pei-Shan Liu, Sheau-Huei Chueh, Chin-Chu Chen, Li-Ya Lee, Li-Yen Shiu
Hericium erinaceus is well known for the neurotrophic effect it confers by promoting nerve growth factor biosynthesis. We discovered a novel bioactivity of H. erinaceus in its ability to suppress adenosine triphosphate (ATP)-induced calcium signaling in neuronal PC12 cells. ATP, known primarily as a neurotransmitter, also acts on purinoceptors (P2 purinergic receptor [P2R]) to generate the cellular calcium signaling and secretion that mediate P2R physiological manifestations, including pain. Chronic pain reduces quality of life...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/29198136/hyperhomocysteinemia-induced-gene-expression-changes-in-the-cell-types-of-the-brain
#12
Erica M Weekman, Abigail E Woolums, Tiffany L Sudduth, Donna M Wilcock
High plasma levels of homocysteine, termed hyperhomocysteinemia, are a risk factor for vascular cognitive impairment and dementia, which is the second leading cause of dementia. While hyperhomocysteinemia induces microhemorrhages and cognitive decline in mice, the specific effect of hyperhomocysteinemia on each cell type remains unknown. We took separate cultures of astrocytes, microglia, endothelial cells, and neuronal cells and treated each with moderate levels of homocysteine for 24, 48, 72, and 96 hr...
November 2017: ASN Neuro
https://www.readbyqxmd.com/read/29196169/purinergic-system-displays-an-anti-inflammatory-profile-in-serum-of-silver-catfish-experimentally-infected-with-streptococcus-agalactiae-an-attempt-to-ameliorate-the-inflammatory-response
#13
Matheus D Baldissera, Carine F Souza, Roberto C V Santos, Bernardo Baldisserotto
The purinergic system is recognized to modulate extracellular adenosine triphosphate (ATP) and related nucleotides through the activities of triphosphate diphosphohydrolase (NTPDase), 5'-nucleotidase, and adenosine deaminase (ADA), thereby playing an essential role in the immunoregulation of inflammatory and immune responses. Thus, the aim of this study was to evaluate whether the purinergic system can improve the inflammatory response in fish experimentally infected with Streptococcus agalactiae through the modulation of seric NTPDase, 5'-nucleotidase and ADA activities...
November 28, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29195019/is-immunosuppression-induced-by-neonatal-thymectomy-compatible-with-poor-reproductive-performance-in-adult-male-rats
#14
M M Ommati, N Tanideh, B Rezakhaniha, J Wang, S Sabouri, M Vahedi, B Dormanesh, O Koohi Hosseinabadi, F Rahmanifar, S Moosapour, A Akhlaghi, R Heidari, M J Zamiri
With increasing knowledge that the immune system has a major impact on reproductive ‎health, the potential for cells arising in organs such as the thymus to alleviate oxidative stress ‎has been revealed. This study addresses the impact of neonatal thymectomy on male ‎reproductive function in pubertal and adult animals. Neonatal Sprague Dawley rats were allotted to four treatments consisting of fully thymectomized, partially thymectomized, intact, and sham-operated rats. Half of the rats in each treatment were sacrificed at 40 and the other half at 80 days of age...
December 1, 2017: Andrology
https://www.readbyqxmd.com/read/29193287/sensitivity-enhancement-for-detection-of-hyperpolarized-13-c-mri-probes-with-1-h-spin-coupling-introduced-by-enzymatic-transformation-in-vivo
#15
Cornelius von Morze, James Tropp, Albert P Chen, Irene Marco-Rius, Mark Van Criekinge, Timothy W Skloss, Daniele Mammoli, John Kurhanewicz, Daniel B Vigneron, Michael A Ohliger, Matthew E Merritt
PURPOSE: Although 1 H spin coupling is generally avoided in probes for hyperpolarized (HP) 13 C MRI, enzymatic transformations of biological interest can introduce large 13 C-1 H couplings in vivo. The purpose of this study was to develop and investigate the application of 1 H decoupling for enhancing the sensitivity for detection of affected HP 13 C metabolic products. METHODS: A standalone 1 H decoupler system and custom concentric 13 C/1 H paddle coil setup were integrated with a clinical 3T MRI scanner for in vivo 13 C MR studies using HP [2-13 C]dihydroxyacetone, a novel sensor of hepatic energy status...
November 28, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29193159/investigation-of-the-quality-of-stored-red-blood-cells-after-simulated-air-drop-in-the-maritime-environment
#16
Athinoula Meli, Vicky Hancock, Heidi Doughty, Steve Smedley, Rebecca Cardigan, Michael Wiltshire
BACKGROUND: Maritime medical capability may be compromised by blood resupply. Air-dropped red blood cells (RBCs) is a possible mitigation factor. This study set out to evaluate RBC storage variables after a simulated parachute air drop into the sea, as limited data exist. STUDY DESIGN AND METHODS: The air load construction for the air drop of blood was subject to static drop assessment to simulate a worst-case parachute drop scenario. One control and two test Golden Hour shipping containers were each packaged with 10 RBC units...
November 27, 2017: Transfusion
https://www.readbyqxmd.com/read/29193157/aeromonas-caviae-inhibits-hepatic-enzymes-of-the-phosphotransfer-network-in-experimentally-infected-silver-catfish-impairment-on-bioenergetics
#17
M D Baldissera, C F Souza, C M Verdi, K L M Dos Santos, M L Da Veiga, M I U M da Rocha, R C V Santos, B S Vizzotto, B Baldisserotto
Several studies have been demonstrated that phosphotransfer network, through the adenylate kinase (AK) and pyruvate kinase (PK) activities, allows for new perspectives leading to understanding of disease conditions associated with disturbances in energy metabolism, metabolic monitoring and signalling. In this sense, the aim of this study was to evaluate whether experimental infection by Aeromonas caviae alters hepatic AK and PK activities of silver catfish Rhamdia quelen. Hepatic AK and PK activities decreased in infected animals compared to uninfected animals, as well as the hepatic adenosine triphosphate (ATP) levels...
November 28, 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29192254/effect-of-src-kinase-inhibition-on-cytochrome-c-smac-diablo-and-apoptosis-inducing-factor-aif-following-cerebral-hypoxia-ischemia-in-newborn-piglets
#18
Panagiotis Kratimenos, Ioannis Koutroulis, Beamon Agarwal, Stamatios Theocharis, Maria Delivoria-Papadopoulos
We have previously shown that cerebral Hypoxia-ischemia (HI) results in activation of Src kinase in the newborn piglet brain. We investigated the regulatory mechanism by which the pre-apoptotic proteins translocate from mitochondria to the cytosol during HI through the Src kinase. Newborn piglets were divided into 3 groups (n = 5/group): normoxic (Nx), HI and HI pre-treated with Src kinase inhibitor PP2 (PP2 + HI). Brain tissue HI was verified by neuropathological analysis and by Adenosine Triphosphate (ATP) and Phosphocreatine (PCr) levels...
November 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29190464/facile-one-pot-fabrication-of-calcium-phosphate-based-composite-nanoparticles-as-delivery-and-mri-contrast-agents-for-macrophages
#19
Maki Nakamura, Ayako Oyane, Kiyoko Kuroiwa, Yoshiki Shimizu, Alexander Pyatenko, Masaki Misawa, Tomokazu Numano, Hisanori Kosuge
We developed a facile one-pot fabrication process for magnetic iron oxide-calcium phosphate (IO-CaP) composite nanoparticles via coprecipitation in labile supersaturated CaP solutions containing IO nanocrystals. All the source solutions used were clinically approved for injection, including water and magnetic IO nanocrystals (ferucarbotran, used as a negative magnetic resonance imaging (MRI) contrast agent). This ensured that the resulting nanoparticles were pathogen- and endotoxin-free. The dispersants used were clinically approved heparin sodium (heparin) or adenosine triphosphate disodium hydrate (ATP), which were added to the IO-containing labile supersaturated CaP solutions...
November 15, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29189956/changes-of-mitochondrial-respiratory-function-during-odontogenic-differentiation-of-rat-dental-papilla-cells
#20
Fuping Zhang, Liulin Jiang, Yifan He, Wenguo Fan, Xiaoyan Guan, Qianyi Deng, Fang Huang, Hongwen He
Dental papilla cells (DPCs) belong to precursor cells differentiating to odontoblasts and play an important role in dentin formation and reproduction. This study aimed to explore the changes and and involvement of mitochondrial respiratory function during odontogenic differentiation. Primary DPCs were obtained from first molar dental papilla of neonatal rats and cultured in odontogenic medium for 7, 14, 21 days. DPCs, which expressed mesenchymal surface markers CD29, CD44 and CD90, had the capacity for self-renewal and multipotent differentiation...
November 30, 2017: Journal of Molecular Histology
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