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https://www.readbyqxmd.com/read/28800517/efficient-inactivation-of-ms-2-virus-in-water-by-hydrodynamic-cavitation
#1
Janez Kosel, Ion Gutiérrez-Aguirre, Nejc Rački, Tanja Dreo, Maja Ravnikar, Matevž Dular
The aim of this study was to accurately quantify the impact of hydrodynamic cavitation on the infectivity of bacteriophage MS2, a norovirus surrogate, and to develop a small scale reactor for testing the effect of hydrodynamic cavitation on human enteric viruses, which cannot be easily prepared in large quantities. For this purpose, 3 mL scale and 1 L scale reactors were constructed and tested. Both devices were efficient in generating hydrodynamic cavitation and in reducing the infectivity of MS2 virus. Furthermore, they reached more than 4 logs reductions of viral infectivity, thus confirming the scalability of hydrodynamic cavitation for this particular application...
July 31, 2017: Water Research
https://www.readbyqxmd.com/read/28795795/mass-defect-based-dimethyl-pyrimidinyl-ornithine-dipyro-tags-for-multiplex-quantitative-proteomics
#2
Dustin C Frost, Amanda R Buchberger, Lingjun Li
We have developed a novel amine-reactive mass defect-based chemical tag, dimethyl pyrimidinyl ornithine (DiPyrO), that is compact in size, is suitable for various biological samples, and enables highly multiplexed quantification of peptides at the MS1 level without increasing mass spectral complexity. The DiPyrO tag structure incorporates heavy isotopes in a variety of configurations to impart as much as 45.3 mDa or as little as 5.8 mDa per tag between labeled peptides. Notably, peptides containing lysine are labeled with two tags, doubling the imparted mass defect to up to 90...
August 10, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28783495/high-flow-ceramic-pot-filters
#3
D van Halem, H van der Laan, A I A Soppe, S G J Heijman
Ceramic pot filters are considered safe, robust and appropriate technologies, but there is a general consensus that water revenues are limited due to clogging of the ceramic element. The objective of this study was to investigate the potential of high flow ceramic pot filters to produce more water without sacrificing their microbial removal efficacy. High flow pot filters, produced by increasing the rice husk content, had a higher initial flow rate (6-19 L h(-1)), but initial LRVs for E. coli of high flow filters was slightly lower than for regular ceramic pot filters...
July 20, 2017: Water Research
https://www.readbyqxmd.com/read/28766289/purification-of-mrna-processing-complexes-using-an-rna-affinity-approach
#4
Xiuye Wang, Yongsheng Shi
Multiple mRNA processing steps, including splicing and 3' processing, take place in macromolecular complexes that contain many proteins and sometimes RNA molecules. A key challenge in the mRNA processing field has been to define the structure-function relationship of these sophisticated molecular machines. A prerequisite for addressing this challenge is to develop tools for purifying mRNA processing complexes in their native and intact forms that are suitable for functional and structural studies. Among many methods that have been developed, RNA affinity-based methods are most widely applied...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28756591/disinfection-of-bacteriophage-ms2-by-copper-in-water
#5
Andrew M Armstrong, Mark D Sobsey, Lisa M Casanova
Households that lack piped water supply are often forced to meet water needs by storing in the home, leaving water vulnerable to contamination by viruses. Storage in copper containers can potentially prevent this type of contamination, but the inactivation kinetics of viruses by copper need to be described to make appropriate storage recommendations. This work characterized inactivation kinetics of bacteriophage MS2 as a surrogate for enteric viruses by dissolved ionic copper in water. Reduction of MS2 increased with increasing doses of copper...
July 30, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28752887/half-metallic-properties-of-3d-transition-metal-atom-intercalated-graphene-ms2-m-w-mo-hybrid-structures
#6
Xiuyun Zhang, Zujian Bao, Xiaoshan Ye, Wenxian Xu, Qiang Wang, Yongjun Liu
The energetics and electronic and magnetic properties of G/MS2 hybrid structures embedded with 3d transition metal atoms, TM@(G/MS2) (G = graphene; M = W, Mo; TM = Sc-Ni), have been systematically studied using first-principles calculations. TM atoms were found to be covalently bound to two-sided graphene and MS2 layers with sizable binding energies of 4.35-7.13 eV. Interestingly, a variety of electronic and magnetic properties were identified for these TM@(G/MS2) systems. Except for TM = Ni, all other systems were ferromagnetic, due to exchange splitting of the TM 3d orbitals...
July 28, 2017: Nanoscale
https://www.readbyqxmd.com/read/28752103/evaluation-of-viral-surrogate-markers-for-study-of-pathogen-dissemination-during-simulations-of-patient-care
#7
Heba Alhmidi, Amrita John, Thriveen C Mana, Sreelatha Koganti, Jennifer L Cadnum, Melissa B Shelton, Curtis J Donskey
During patient care simulations, cauliflower mosaic virus DNA and bacteriophage MS2 performed similarly as surrogate markers of pathogen dissemination. These markers disseminated to the environment in a manner similar to Clostridium difficile spores but were more frequently detected on skin and clothing of personnel after personal protective equipment removal.
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28750330/modelling-the-transport-and-decay-processes-of-microbial-tracers-in-a-macro-tidal-estuary
#8
Amyrhul Abu-Bakar, Reza Ahmadian, Roger A Falconer
The Loughor Estuary is a macro-tidal coastal basin, located along the Bristol Channel, in the South West of the U.K. The maximum spring tidal range in the estuary is up to 7.5 m, near Burry Port Harbour. This estuarine region can experience severe coastal flooding during high spring tides, including extreme flooding of the intertidal saltmarshes at Llanrhidian, as well as the lower industrial and residential areas at Llanelli and Gowerton. The water quality of this estuarine basin needs to comply with the designated standards for safe recreational bathing and shellfish harvesting industries...
July 3, 2017: Water Research
https://www.readbyqxmd.com/read/28741854/toward-activity-origin-of-electrocatalytic-hydrogen-evolution-reaction-on-carbon-rich-crystalline-coordination-polymers
#9
Lihuan Wang, Diana C Tranca, Jian Zhang, Yanpeng Qi, Stavroula Sfaelou, Tao Zhang, Renhao Dong, Xiaodong Zhuang, Zhikun Zheng, Gotthard Seifert
The fundamental understanding of electrocatalytic active sites for hydrogen evolution reaction (HER) is significantly important for the development of metal complex involved carbon electrocatalysts with low kinetic barrier. Here, the MSx Ny (M = Fe, Co, and Ni, x/y are 2/2, 0/4, and 4/0, respectively) active centers are immobilized into ladder-type, highly crystalline coordination polymers as model carbon-rich electrocatalysts for H2 generation in acid solution. The electrocatalytic HER tests reveal that the coordination of metal, sulfur, and nitrogen synergistically facilitates the hydrogen ad-/desorption on MSx Ny catalysts, leading to enhanced HER kinetics...
July 25, 2017: Small
https://www.readbyqxmd.com/read/28738697/reduction-of-heart-rate-variability-in-hypertensive-elderly
#10
Paulo Evaristo de Andrade, Joice Anaize Tonon do Amaral, Laércio da Silva Paiva, Fernando Adami, Juliana Zangirolami Raimudo, Vitor Engrácia Valenti, Luiz Carlos de Abreu, Rodrigo Daminello Raimundo
INTRODUCTION: The elderly population grows globally every day, and along with it the incidence of high blood pressure also grows, following these data scientists seek tools to improve the health of this population, a widely used tool for assessing cardiovascular function is the heart rate variability(HRV), that is a mecanism that allows the investigation of oscillations in the intervals between consecutive heart beats (RR intervals). There are already studies on the autonomic nervous system in the elderly population, however, these studies investigated normotensive patients and there is little research in hypertensive patients...
July 24, 2017: Blood Pressure
https://www.readbyqxmd.com/read/28738147/round-robin-study-molecular-simulation-of-thermodynamic-properties-from-models-with-internal-degrees-of-freedom
#11
Michael Schappals, Andreas Mecklenfeld, Leif Kröger, Vitalie Botan, Andreas Köster, Simon Stephan, Edder J García, Gabor Rutkai, Gabriele Raabe, Peter Klein, Kai Leonhard, Colin W Glass, Johannes Lenhard, Jadran Vrabec, Hans Hasse
Thermodynamic properties are often modeled by classical force fields which describe the interactions on the atomistic scale. Molecular simulations are used for retrieving thermodynamic data from such models, and many simulation techniques and computer codes are available for that purpose. In the present round robin study, the following fundamental question is addressed: Will different user groups working with different simulation codes obtain coinciding results within the statistical uncertainty of their data? A set of 24 simple simulation tasks is defined and solved by five user groups working with eight molecular simulation codes: DL_POLY, GROMACS, IMC, LAMMPS, ms2, NAMD, Tinker, and TOWHEE...
August 7, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28737905/metabolism-of-t-2-toxin-in-farm-animals-and-human-in-vitro-and-in-chickens-in-vivo-using-ultra-high-performance-liquid-chromatography-quadrupole-time-of-flight-hybrid-mass-spectrometry-along-with-online-hydrogen-deuterium-exchange-technique
#12
Shupeng Yang, Marthe De Boevre, Huiyan Zhang, Karl De Ruyck, Feifei Sun, Jinzhen Zhang, Yue Jin, Yanshen Li, Zhanhui Wang, Suxia Zhang, Jinhui Zhou, Yi Li, Sarah De Saeger
After incubated with animals' and human liver microsomes, metabolites of phase I and II were investigated. A comparison was performed by ultrahigh performance liquid chromatography-quadrupole/time-of-flight coupled to mass spectrometry (UHPLC-Q/TOF). Consequently, a total of four phase I metabolites and three glucuronide binding metabolites of T-2 toxin were discovered. Although a significant metabolic difference was observed among six species, HT-2 toxin was the major product in all species. In addition, the in vivo metabolism of T-2 toxin after oral administration was also investigated in chickens, In total, 18 metabolites were detected, of which 13 were novel, to our knowledge, and reported for the first time...
July 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28734974/the-effects-of-pneumoperitoneum-and-the-steep-trendelenburg-position-on-heart-rate-variability-and-cerebral-oxygenation-during-robotic-sacrocolpopexy
#13
Emad Matanes, Amir Weissman, Aleksandr Rivlin, Roy Lauterbach, Amnon Amit, Zeev Wiener, Lior Lowenstein
STUDY OBJECTIVE: The aim of the current study was to investigate how steep Trendelenburg positioning with pneumoperitoneum modifies brain oxygenation and autonomic nervous system modulation of heart rate variability during robotic sacrocolpopexy. DESIGN: A 5-minute computerized ECG, cerebral O2 saturation (cSO2), systemic O2 saturation (sSO2), heart rate (HR), diastolic blood pressure (BP), systolic BP and end tidal CO2 tension were recorded immediately after anesthesia induction (baseline phase) and after alterations in positioning and in intra-abdominal pressure...
July 19, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28721617/effect-of-membrane-property-and-feed-water-organic-matter-quality-on-long-term-performance-of-the-gravity-driven-membrane-filtration-process
#14
Dongwhi Lee, Yuri Lee, Shin Sik Choi, Sang-Ho Lee, Kyoung-Woong Kim, Yunho Lee
This study investigated the influence of membrane property and feed water organic matter quality on the permeate flux and water quality during gravity-driven membrane (GDM) filtration. GDM filtration was continuously carried out over 500 days at hydrostatic pressure of 65 mbar in dead-end mode without any back-flushing or membrane cleaning. Three ultrafiltration (UF) membranes (PES-100 kDa, PVDF-120 kDa, and PVDF-100 kDa) and one microfiltration (MF) membrane (PTFE-0.3 μm) were tested for treating lake water with varied organic matter qualities due to algal growth...
July 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28720444/characterization-of-a-spray-dried-candidate-hpv-l2-vlp-vaccine-stored-for-multiple-years-at-room-temperature
#15
Julianne Peabody, Pavan Muttil, Bryce Chackerian, Ebenezer Tumban
HPV infections are associated with human cancers. Although three prophylactic vaccines have been approved to protect against HPV infections, the vaccines require cold-chain storage and may not be suitable for third world countries with less developed refrigeration facilities. We previously developed a bacteriophage L2 virus-like particle (VLP)-based candidate vaccine, which elicited broadly protective antibodies against diverse HPV types. Spray-drying of MS2-16L2 VLPs into a dry powder enhanced the stability of these VLPs...
June 2017: Papillomavirus Research
https://www.readbyqxmd.com/read/28716310/the-role-of-same-sex-mentorship-and-organizational-support-in-encouraging-women-to-pursue-surgery
#16
Erynne A Faucett, Hilary C McCrary, Tijana Milinic, Tania Hassanzadeh, Simin G Roward, Leigh A Neumayer
INTRODUCTION: While women represent approximately half of all medical students, only 38% of general surgery residents are women. The objective of this study is to explore how access to mentors and organizational support affects career choices. METHODS: In June of 2016, a survey was sent to medical students at a single institution (n = 472). Questions utilized a 5-point Likert scale. A two-sample t-test was used to evaluate data. RESULTS: A total of 160 students participated in the survey...
July 12, 2017: American Journal of Surgery
https://www.readbyqxmd.com/read/28708940/the-street-like-synthesis-of-krokodil-results-in-the-formation-of-an-enlarged-cluster-of-known-and-new-morphinans-with-therapeutic-potential
#17
José Soares, Emanuele Alves, André Silva, Natália Figueiredo, João F Neves, Sara Cravo, Maria Rangel, Annibal Netto, Félix Carvalho, Ricardo Jorge Dinis-Oliveira, Carlos Afonso
"Krokodil" is the street name for a homemade injectable drug that has been used as a cheap substitute for heroin. Codeine is the opioid starting material for krokodil synthesis and desomorphine is the opioid claimed to be the main component of krokodil and the main responsible for its addictive and psychoactive characteristics. However, due to its peculiar manufacture, using cheap raw materials, krokodil is composed by a large and complex mixture of different substances. In order to shed some light upon the chemical complexity of krokodil, its profiling was conducted by reverse phase high performance liquid chromatography coupled to photodiode array detector (RP-HPLC-DAD) and by liquid chromatography coupled to high resolution tandem mass spectrometry (LC-ESI-IT-Orbitrap-MS)...
July 14, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28694238/functionalized-bioengineered-spider-silk-spheres-improve-nuclease-resistance-and-activity-of-oligonucleotide-therapeutics-providing-a-strategy-for-cancer-treatment
#18
Anna Karolina Kozlowska, Anna Florczak, Maciej Smialek, Ewelina Dondajewska, Andrzej Mackiewicz, Marcin Kortylewski, Hanna Dams-Kozlowska
Cell-selective delivery and sensitivity to serum nucleases remain major hurdles to the clinical application of RNA-based oligonucleotide therapeutics, such as siRNA. Spider silk shows great potential as a biomaterial due to its biocompatibility and biodegradability. Self-assembling properties of silk proteins allow for processing into several different morphologies such as fibers, scaffolds, films, hydrogels, capsules and spheres. Moreover, bioengineering of spider silk protein sequences can functionalize silk by adding peptide moieties with specific features including binding or cell recognition domains...
September 1, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28691656/mutational-analysis-of-the-ms2-lysis-protein-l
#19
Karthik R Chamakura, Garrett B Edwards, Ry Young
Small single-stranded nucleic acid phages effect lysis by expressing a single protein, the amurin, lacking muralytic enzymatic activity. Three amurins have been shown to act like 'protein antibiotics' by inhibiting cell-wall biosynthesis. However, the L lysis protein of the canonical ssRNA phage MS2, a 75 aa polypeptide, causes lysis by an unknown mechanism without affecting net peptidoglycan synthesis. To identify residues important for lytic function, randomly mutagenized alleles of L were generated, cloned into an inducible plasmid and the transformants were selected on agar containing the inducer...
July 8, 2017: Microbiology
https://www.readbyqxmd.com/read/28690114/maintenance-of-miranda-localization-in-drosophila-neuroblasts-involves-interaction-with-the-cognate-mrna
#20
Anne Ramat, Matthew Hannaford, Jens Januschke
How cells position their proteins is a key problem in cell biology. Targeting mRNAs to distinct regions of the cytoplasm contributes to protein localization by providing local control over translation. Here, we reveal that an interdependence of a protein and cognate mRNA maintains asymmetric protein distribution in mitotic Drosophila neural stem cells. We tagged endogenous mRNA or protein products of the gene miranda that is required for fate determination with GFP. We find that the mRNA localizes like the protein it encodes in a basal crescent in mitosis...
July 24, 2017: Current Biology: CB
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