keyword
MENU ▼
Read by QxMD icon Read
search

MS2

keyword
https://www.readbyqxmd.com/read/28651198/the-toxicity-of-cationic-surfactant-hdtma-br-desorbed-from-surfactant-modified-zeolite-towards-faecal-indicator-and-environmental-microorganisms
#1
Peter J Reeve, Howard J Fallowfield
Surfactant Modified Zeolite (SMZ) represents a versatile, cost-effective permeable reactive material, capable of treating multiple classes of contaminants. The potential for HDTMA-Br, a cationic surfactant commonly used to modify zeolite, to desorb from the zeolite surface has been identified as a potential issue for the ongoing use of SMZ in water remediation contexts. This paper investigates the toxicity of HDTMA-Br towards enteric virus surrogates, F-RNA bacteriophage MS2 and E. coli, Bacillus subtilis, and soil microflora...
June 13, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28650715/transfer-of-bacteriophage-ms2-and-fluorescein-from-n95-filtering-facepiece-respirators-to-hands-measuring-fomite-potential
#2
Tyler M Brady, Amanda L Strauch, Claudia M Almaguer, George Niezgoda, Ronald E Shaffer, Patrick L Yorio, Edward M Fisher
Contact transmission of pathogens from personal protective equipment is a concern within the healthcare industry. During public health emergency outbreaks, resources become constrained and the reuse of personal protective equipment, such as N95 filtering facepiece respirators, may be needed. This study was designed to characterize the transfer of bacteriophage MS2 and fluorescein between filtering facepiece respirators and the wearer's hands during three simulated use scenarios. Filtering facepiece respirators were contaminated with MS2 and fluorescein in droplets or droplet nuclei...
June 26, 2017: Journal of Occupational and Environmental Hygiene
https://www.readbyqxmd.com/read/28644610/mzmine-2-data-preprocessing-to-enhance-molecular-networking-reliability
#3
Florent Olivon, Gwendal Grelier, Fanny Roussi, Marc Litaudon, David Touboul
Molecular networking is becoming more and more popular into the metabolomic community to organize tandem mass spectrometry (MS2) data. Even though this approach allows the treatment and comparison of large data sets, several drawbacks related to the MS-Cluster tool routinely used on the Global Natural Product Social Molecular Networking platform (GNPS) limit its potential. MS-Cluster cannot distinguish between chromatography well-resolved isomers as retention times are not taken into account. Annotation with predicted chemical formulas is also not implemented and semi-quantification is only based on the number of MS2 scans...
June 23, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28624537/visualizing-rna-granule-transport-and-translation-in-living-neurons
#4
REVIEW
Karl E Bauer, Michael A Kiebler, Inmaculada Segura
In polarized cells, such as neurons, the synthesis of an mRNA does not ensure its proper cellular expression. Most mature transcripts require the association with RNA-binding proteins, resulting in the formation of RNA granules, which are then transported within the cytoplasm along the cytoskeleton and delivered to their proper subcellular locations, where they can be locally translated. Here we review current microscopy methods that have been developed to visualize RNA granule formation, transport and translation at the single cell level with a special emphasis on the MS2 and SunTag systems...
June 14, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28616834/minimizing-bias-in-virally-seeded-water-treatment-studies-evaluation-of-optimal-bacteriophage-and-mammalian-virus-preparation-methodologies
#5
Nathan Dunkin, ShihChi Weng, Joseph G Jacangelo, Kellogg J Schwab
One key assumption impacting data quality in viral inactivation studies is that reduction estimates are not altered by the virus seeding process. However, seeding viruses often involves the inadvertent addition of co-constituents such as cell culture components or additives used during preparation steps which can impact viral reduction estimates by inducing non-representative oxidant demand in disinfection studies and fouling in membrane assessments. The objective of this study was therefore to characterize a mammalian norovirus surrogate, murine norovirus (MNV), and bacteriophage MS2 at sequential stages of viral purification and to quantify their potential contribution to artificial oxidant demand and non-representative membrane fouling...
June 14, 2017: Food and Environmental Virology
https://www.readbyqxmd.com/read/28616833/use-of-preservative-agents-and-antibiotics-for-increased-poliovirus-survival-on-positively-charged-filters
#6
Christine Susan Fagnant, Alexandra Lynn Kossik, Nicolette Angela Zhou, Liliana Sánchez-Gonzalez, Jill Christin Falman, Erika Karen Keim, Yarrow Linden, Alana Scheibe, Kilala Sayisha Barnes, Nicola Koren Beck, David S Boyle, John Scott Meschke
Environmental surveillance of poliovirus (PV) and other non-enveloped viruses can help identify silent circulation and is necessary to certify eradication. The bag-mediated filtration system is an efficient method to filter large volumes of environmental waters at field sites for monitoring the presence of viruses. As filters may require long transit times to off-site laboratories for processing, viral inactivation or overgrowth of bacteria and fungi can interfere with virus detection and quantification (Miki and Jacquet in Aquatic Microb Ecol 51(2):195-208, 2008)...
June 14, 2017: Food and Environmental Virology
https://www.readbyqxmd.com/read/28614337/dual-axis-optical-coherence-tomography-for-deep-tissue-imaging
#7
Yang Zhao, Will J Eldridge, Jason R Maher, Sanghoon Kim, Michael Crose, Mohamed Ibrahim, Howard Levinson, Adam Wax
We have developed dual-axis optical coherence tomography (DA-OCT) which enables deep tissue imaging by using a novel off-axis illumination/detection configuration. DA-OCT offers a 100-fold speed increase compared with its predecessor, multispectral multiple-scattering low coherence interferometry (ms2/LCI), by using a new beam scanning mechanism based on a microelectro-mechanical system (MEMS) mirror. The data acquisition scheme was altered to take advantage of this scanning speed, producing tomographic images at a rate of 4 frames (B-scans) per second...
June 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28606192/assessment-of-healthcare-worker-protocol-deviations-and-self-contamination-during-personal-protective-equipment-donning-and-doffing
#8
Jennie H Kwon, Carey-Ann D Burnham, Kimberly A Reske, Stephen Y Liang, Tiffany Hink, Meghan A Wallace, Angela Shupe, Sondra Seiler, Candice Cass, Victoria J Fraser, Erik R Dubberke
OBJECTIVE To evaluate healthcare worker (HCW) risk of self-contamination when donning and doffing personal protective equipment (PPE) using fluorescence and MS2 bacteriophage. DESIGN Prospective pilot study. SETTING Tertiary-care hospital. PARTICIPANTS A total of 36 HCWs were included in this study: 18 donned/doffed contact precaution (CP) PPE and 18 donned/doffed Ebola virus disease (EVD) PPE. INTERVENTIONS HCWs donned PPE according to standard protocols. Fluorescent liquid and MS2 bacteriophage were applied to HCWs...
June 13, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28596759/bsocial-deciphering-social-behaviors-within-mixed-microbial-populations
#9
Jessica Purswani, Rocío C Romero-Zaliz, Antonio M Martín-Platero, Isabel M Guisado, Jesús González-López, Clementina Pozo
Ecosystem functionality depends on interactions among populations, of the same or different taxa, and these are not just the sum of pairwise interactions. Thus, know-how of the social interactions occurring in mixed-populations are of high interest, however they are commonly unknown due to the limitations posed in tagging each population. The limitations include costs/time in tediously fluorescent tagging, and the number of different fluorescent tags. Tag-free strategies exist, such as high-throughput sequencing, but ultimately both strategies require the use of expensive machinery...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28591313/multiplexed-reverse-transcription-real-time-polymerase-chain-reaction-for-simultaneous-detection-of-mayaro-oropouche-and-oropouche-like-viruses
#10
Felipe Gomes Naveca, Valdinete Alves do Nascimento, Victor Costa de Souza, Bruno Tardelli Diniz Nunes, Daniela Sueli Guerreiro Rodrigues, Pedro Fernando da Costa Vasconcelos
We describe a sensitive method for simultaneous detection of Oropouche and Oropouche-like viruses carrying the Oropouche S segment, as well as the Mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (RT-qPCR). A chimeric plasmid containing both Mayaro and Oropouche targets was designed and evaluated for the in vitro production of transcribed RNA, which could be easily used as a non-infectious external control. To track false-negative results due to PCR inhibition or equipment malfunction, the MS2 bacteriophage was also included in the multiplex assay as an internal positive control...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28582591/a-tool-for-integrating-genetic-and-mass-spectrometry-based-peptide-data-proteogenomics-viewer-pv-a-genome-browser-like-tool-which-includes-ms-data-visualization-and-peptide-identification-parameters
#11
REVIEW
José Eduardo Kroll, Vandeclécio Lira da Silva, Sandro José de Souza, Gustavo Antonio de Souza
In this manuscript we describe Proteogenomics Viewer, a web-based tool that collects MS peptide identification, indexes to genomic sequence and structure, assigns exon usage, reports the identified protein isoforms with genomic alignments and, most importantly, allows the inspection of MS2 information for proper peptide identification. It also provides all performed indexing to facilitate global analysis of the data. The relevance of such tool is that there has been an increase in the number of proteogenomic efforts to improve the annotation of both genomics and proteomics data, culminating with the release of the two human proteome drafts...
June 5, 2017: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
https://www.readbyqxmd.com/read/28581757/impact-of-hydrogen-bonding-in-the-binding-site-between-capsid-protein-and-ms2-bacteriophage-ssrna
#12
Lokendra Poudel, Reidun Twarock, Nicole F Steinmetz, Rudolf Podgornik, Wai-Yim Ching
MS2 presents a well-studied example of a single-stranded RNA virus for which the genomic RNA plays a pivotal role in the virus assembly process based on the packaging signal-mediated mechanism. Packaging signals (PSs) are multiple dispersed RNA sequence/structure motifs varying around a central recognition motif that interact in a specific way with the capsid protein in the assembly process. Although the discovery and identification of these PSs was based on bioinformatics and geometric approaches, in tandem with sophisticated experimental protocols, we approach this problem using large-scale ab initio computation centered on critical aspects of the consensus protein-RNA interactions recognition motif...
June 21, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28581703/anion-exchange-chromatography-coupled-to-high-resolution-mass-spectrometry-a-powerful-tool-for-merging-targeted-and-non-targeted-metabolomics
#13
Michaela Schwaiger, Evelyn Rampler, Gerrit Hermann, Walter Miklos, Walter Berger, Gunda Koellensperger
In this work, simultaneous targeted metabolic profiling by isotope dilution and non-targeted fingerprinting is proposed for cancer cell studies. The novel streamlined metabolomics workflow was established using anion-exchange chromatography (IC) coupled to high-resolution mass spectrometry (MS). The separation time of strong anion-exchange (2 mm column, flow rate 380 μL min(-1), injection volume 5 μL) could be decreased to 25 min for a target list comprising organic acids, sugars, sugar phosphates, and nucleotides...
June 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28573295/what-shapes-eukaryotic-transcriptional-bursting
#14
REVIEW
Damien Nicolas, Nick E Phillips, Felix Naef
Isogenic cells in a common environment present a large degree of heterogeneity in gene expression. Part of this variability is attributed to transcriptional bursting: the stochastic activation and inactivation of promoters that leads to the discontinuous production of mRNA. The diversity in bursting patterns displayed by different genes suggests the existence of a connection between bursting and gene regulation. Experimental strategies such as single-molecule RNA FISH, MS2-GFP or short-lived protein reporters allow the quantification of transcriptional bursting and the comparison of bursting kinetics between conditions, allowing therefore the identification of molecular mechanisms modulating transcriptional bursting...
June 27, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28558089/analysis-of-heart-rate-variability-before-and-during-tilt-test-in-patients-with-cardioinhibitory-vasovagal-syncope
#15
Cláudia Madeira Miranda, Rose Mary Ferreira Lisboa da Silva
BACKGROUND: Cardioinhibitory vasovagal response is uncommon during the tilt test (TT). Heart rate variability (HRV) by use of spectral analysis can distinguish patients with that response. OBJECTIVE: To compare the HRV in patients with cardioinhibitory vasovagal syncope (case group - G1) with that in patients without syncope and with negative response to TT (control group - G2). METHODS: 64 patients were evaluated (mean age, 36.2 years; 35 men) and submitted to TT at 70 degrees, under digital Holter monitoring...
December 2016: Arquivos Brasileiros de Cardiologia
https://www.readbyqxmd.com/read/28557624/rna-guided-activation-of-pluripotency-genes-in-human-fibroblasts
#16
Kai Xiong, Yan Zhou, Kristian Aabo Blichfeld, Poul Hyttel, Lars Bolund, Kristine Karla Freude, Yonglun Luo
Specific activation of endogenous genes can be achieved by programmable artificial transcription factors (ATFs). In this study, we compared two artificial, programmable, clustered regularly interspaced short palindromic repeats (CRISPR)-based, ubiquitous transcription factors: deficient CRISPR-associated protein 9 (dCas9)-VP64 (CRISPRa) alone, or a combination of dCas9-VP64 and MS2-P65-HSF1 [synergistic activation mediator (SAM) system] mediated activation of five pluripotency genes: KLF4 (K), LIN28 (L), MYC (M), OCT4 (O), and SOX2 (S) in human cells (HEK293T, HeLa, HepG2, and primary fibroblasts)...
June 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28553088/blood-pressure-reactivity-to-mental-stress-is-attenuated-following-resistance-exercise-in-older-hypertensive-women
#17
Rafael Gauche, Ricardo M Lima, Jonathan Myers, André B Gadelha, Silvia Gr Neri, Claudia Lm Forjaz, Lauro C Vianna
PURPOSE: This study aimed to investigate the effects of resistance exercise (RE) on autonomic control and blood pressure (BP) reactivity during mental stress (MS) in treated older hypertensive women. METHODS: Ten older hypertensive women (age =71.1±5.5 years; body mass index =24.2±3.9; mean BP [MBP] =85.4±3.5) underwent a protocol consisting of BP and heart rate variability (HRV) output assessments at baseline and during MS, and these measurements were taken before and 60 minutes after two bouts of RE (traditional and circuit)...
2017: Clinical Interventions in Aging
https://www.readbyqxmd.com/read/28551509/bacteriophage-removal-efficiency-as-a-validation-and-operational-monitoring-tool-for-virus-reduction-in-wastewater-reclamation-review
#18
REVIEW
Mohan Amarasiri, Masaaki Kitajima, Thanh H Nguyen, Satoshi Okabe, Daisuke Sano
The multiple-barrier concept is widely employed in international and domestic guidelines for wastewater reclamation and reuse for microbiological risk management, in which a wastewater reclamation system is designed to achieve guideline values of the performance target of microbe reduction. Enteric viruses are one of the pathogens for which the target reduction values are stipulated in guidelines, but frequent monitoring to validate human virus removal efficacy is challenging in a daily operation due to the cumbersome procedures for virus quantification in wastewater...
May 19, 2017: Water Research
https://www.readbyqxmd.com/read/28531182/selection-of-a-biosafety-level-1-bsl-1-surrogate-to-evaluate-surface-disinfection-efficacy-in-ebola-outbreaks-comparison-of-four-bacteriophages
#19
Karin Gallandat, Daniele Lantagne
The 2014 West African Ebola virus disease outbreak was the largest to date, and conflicting, chlorine-based surface disinfection protocols to interrupt disease transmission were recommended. We identified only one study documenting surface disinfection efficacy against the Ebola virus, showing a >6.6 log reduction after 5-minute exposure to 0.5% sodium hypochlorite (NaOCl) based on small-scale tests (Cook et al. (2015)). In preparation for future extensive, large-scale disinfection efficacy experiments, we replicated the Cook et al...
2017: PloS One
https://www.readbyqxmd.com/read/28529972/simple-and-versatile-detection-of-viruses-using-anodized-alumina-membranes
#20
Pavan Chaturvedi, Stacy D Rodriguez, Ivan Vlassiouk, Immo A Hansen, Sergei N Smirnov
A simple sensor for viral particles based on ionic conductivity through anodized alumina membranes was demonstrated using MS2 bacteriophage as an example. A facile two-point measuring scheme is geared toward realization using a computer's sound card input/output capabilities suitable for a fast and inexpensive point of care testing. The lowest detection concentration down to ~7 pfu/mL and a large dynamic range up to ~2000 pfu/mL were obtained due to physical optimization that included proper length and diameter for the pores, removing the oxide layer at the electrode, as well as the chemical optimization of covalent binding of antibodies to the pore's walls...
May 27, 2016: ACS Sensors
keyword
keyword
5174
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"