keyword
MENU ▼
Read by QxMD icon Read
search

MS2

keyword
https://www.readbyqxmd.com/read/28105392/in-vivo-long-term-biodistribution-excretion-and-toxicology-of-pegylated-transition-metal-dichalcogenides-ms2-m-mo-w-ti-nanosheets
#1
Jiali Hao, Guosheng Song, Teng Liu, Xuan Yi, Kai Yang, Liang Cheng, Zhuang Liu
With unique 2D structures and intriguing physicochemical properties, various types of transition metal dichalcogenides (TMDCs) have attracted much attention in many fields including nanomedicine. Hence, it is of great importance to carefully study the in vivo biodistribution, excretion, and toxicology profiles of different TMDCs, and hopefully to identify the most promising type of TMDCs with low toxicity and fast excretion for further biomedical applications. Herein, the in vivo behaviors of three representative TMDCs including molybdenum dichalcogenides (MoS2), tungsten dichalcogenides (WS2), and titanium dichalcogenides (TiS2) nanosheets are systematically investigated...
January 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/28102081/deep-coverage-of-global-protein-expression-and-phosphorylation-in-breast-tumor-cell-lines-using-tmt-10-plex-isobaric-labeling
#2
Fang-Ke Huang, Guoan Zhang, Kevin Lawlor, Arpi Nazarian, John Philip, Paul Tempst, Noah Dephoure, Thomas A Neubert
Labeling peptides with isobaric tags is a popular strategy in quantitative bottom-up proteomics. In this study, we labeled six breast tumor cell lysates (1.34 mg proteins per channel) using 10-plex tandem mass tag reagents and analyzed the samples on a Q Exactive HF Quadrupole-Orbitrap mass spectrometer. We identified a total of 8706 proteins and 28186 phosphopeptides, including 7394 proteins and 23739 phosphosites common to all channels. The majority of technical replicates correlated with a R2 ≥ 0.98, indicating minimum variability was introduced after labeling...
January 19, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28096443/stem-loop-rna-labeling-can-affect-nuclear-and-cytoplasmic-mrna-processing
#3
Stephanie Heinrich, Corinne L Sidler, Claus M Azzalin, Karsten Weis
The binding of sequence-specific RNA-interacting proteins, such as the bacteriophage MS2 or PP7 coat proteins, to their corresponding target sequences has been extremely useful and widely used to visualize single mRNAs in vivo. However, introduction of MS2 stem-loops into yeast mRNAs has recently been shown to lead to the accumulation of RNA fragments, suggesting that the loops impair mRNA decay. This result was questioned, because fragment occurrence was mainly assessed using ensemble methods, and their cellular localization and its implications had not been addressed on a single transcript level...
February 2017: RNA
https://www.readbyqxmd.com/read/28095510/screening-and-identifying-antioxidative-components-in-ginkgo-biloba-pollen-by-dpph-hplc-pad-coupled-with-hplc-esi-ms2
#4
Jiying Qiu, Xiangyan Chen, A I Netrusov, Qingxin Zhou, Danyang Guo, Xiaoyong Liu, Hailun He, Xue Xin, Yifen Wang, Leilei Chen
The Ginkgo biloba is one of ancient trees that exists from billions of years ago, its leaf and nut are used as herbs and foods in China, while so far its pollen does not have any application except pollination. In order to evaluate the antioxidant activity of Ginkgo biloba pollen, and rapidly screen its antioxidative components, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, total flavonoid, total phenol, and proanthocyanidin of Ginkgo biloba pollen were determined and compared with those of Ginkgo biloba leaf and nut, and the off-line DPPH-HPLC-PAD and HPLC-ESI-MS2 were applied for screening and identifying the antioxidant flavonoids in Ginkgo biloba pollen...
2017: PloS One
https://www.readbyqxmd.com/read/28093213/characterization-of-the-efficiency-and-uncertainty-of-skimmed-milk-flocculation-for-the-simultaneous-concentration-and-quantification-of-water-borne-viruses-bacteria-and-protozoa
#5
Eloy Gonzales-Gustavson, Yexenia Cardenas-Youngs, Miquel Calvo, Marcelle Figueira Marques da Silva, Ayalkibet Hundesa, Inmaculada Amorós, Yolanda Moreno, Laura Moreno-Mesonero, Rosa Rosell, Lliliane Ganges, Rosa Araujo, Rosina Girones
In this study, the use of skimmed milk flocculation (SMF) to simultaneously concentrate viruses, bacteria and protozoa was evaluated. We selected strains of faecal indicator bacteria and pathogens, such as Escherichia coli and Helicobacter pylori. The viruses selected were adenovirus (HAdV 35), rotavirus (RoV SA-11), the bacteriophage MS2 and bovine viral diarrhoea virus (BVDV). The protozoa tested were Acanthamoeba, Giardia and Cryptosporidium. The mean recoveries with q(RT)PCR were 66% (HAdV 35), 24% (MS2), 28% (RoV SA-11), 15% (BVDV), 60% (E...
January 13, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28088300/cytoplasmic-rna-protein-particles-exhibit-non-gaussian-subdiffusive-behavior
#6
Thomas J Lampo, Stella Stylianidou, Mikael P Backlund, Paul A Wiggins, Andrew J Spakowitz
The cellular cytoplasm is a complex, heterogeneous environment (both spatially and temporally) that exhibits viscoelastic behavior. To further develop our quantitative insight into cellular transport, we analyze data sets of mRNA molecules fluorescently labeled with MS2-GFP tracked in real time in live Escherichia coli and Saccharomyces cerevisiae cells. As shown previously, these RNA-protein particles exhibit subdiffusive behavior that is viscoelastic in its origin. Examining the ensemble of particle displacements reveals a Laplace distribution at all observed timescales rather than the Gaussian distribution predicted by the central limit theorem...
January 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28075408/time-course-exo-metabolomic-profiling-in-the-green-marine-macroalga-ulva-chlorophyta-for-identification-of-growth-phase-dependent-biomarkers
#7
Taghreed Alsufyani, Anne Weiss, Thomas Wichard
The marine green macroalga Ulva (Chlorophyta) lives in a mutualistic symbiosis with bacteria that influence growth, development, and morphogenesis. We surveyed changes in Ulva's chemosphere, which was defined as a space where organisms interact with each other via compounds, such as infochemicals, nutrients, morphogens, and defense compounds. Thereby, Ulva mutabilis cooperates with bacteria, in particular, Roseovarius sp. strain MS2 and Maribacter sp. strain MS6 (formerly identified as Roseobacter sp. strain MS2 and Cytophaga sp...
January 10, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28032985/redox-exfoliation-of-layered-transition-metal-dichalcogenides
#8
Ali Jawaid, Justin Che, Lawrence F Drummy, John Bultman, Adam Waite, Ming-Siao Hsiao, Richard A Vaia
Transition Metal Dichalcogenides (TMDs) have attracted considerable attention in a diverse array of applications due to the breadth of possible property suites relative to other low dimensional nanomaterials (e.g. graphene, aluminosilicates). Here, we demonstrate an alternative methodology for the exfoliation of bulk crystallites of Group V-VII layered TMDs under quiescent, bench-top conditions using mild redox chemistry. Anionic polyoxometalate species generated from edge sites adsorb to the TMD surface and create coulombic repulsion that drives layer separation without the use of shear forces...
December 29, 2016: ACS Nano
https://www.readbyqxmd.com/read/28031758/somatic-endurance-performance-and-heart-rate-variability-profiles-of-professional-soccer-players-grouped-according-to-age
#9
Michal Botek, Jakub Krejčí, Andrew J McKune, Iva Klimešová
This cross-sectional study compared somatic, endurance performance determinants and heart rate variability (HRV) profiles of professional soccer players divided into different age groups: GI (17-19.9 years; n = 23), GII (20-24.9 years; n = 45), GIII (25-29.9 years; n = 30), and GIV (30-39 years; n = 26). Players underwent somatic and HRV assessment and maximal exercise testing. HRV was analyzed by spectral analysis of HRV, and high (HF) and low (LF) frequency power was transformed by a natural logarithm (Ln)...
December 1, 2016: Journal of Human Kinetics
https://www.readbyqxmd.com/read/28030577/resistance-of-aerosolized-bacterial-viruses-to-four-germicidal-products
#10
Nathalie Turgeon, Kevin Michel, Thi-Lan Ha, Enric Robine, Sylvain Moineau, Caroline Duchaine
Viral diseases can spread through a variety of routes including aerosols. Yet, limited data are available on the efficacy of aerosolized chemicals to reduce viral loads in the air. Bacteriophages (phages) are often used as surrogates for hazardous viruses in aerosol studies because they are inexpensive, easy to handle, and safe for laboratory workers. Moreover, several of these bacterial viruses display physical characteristics similar to pathogenic human and animal viruses, like morphological size, type of nucleic acids, capsid morphology, and the presence of an envelope...
2016: PloS One
https://www.readbyqxmd.com/read/28017721/the-role-of-the-loop-1-region-in-mazfbs-mrna-interferase-from-bacillus-subtilis-in-recognition-of-the-3-end-of-the-rna-substrate
#11
Yojiro Ishida, Keiko Inouye, Masayori Inouye
MazFbs is an mRNA interferase from Bacillus subtilis specifically recognizing UACAU. The X-ray structure of its complex with an RNA substrate has been also solved. When its amino acid sequence is compared with that of MazFhw, an mRNA interferase from a highly halophilic archaeon, recognizing UUACUCA, the 9-residue loop-1 region is highly homologous except that the V16V17 sequence in MazFbs is replaced with TK in MazFhw. Thus, we examined the role of the VV sequence in RNA substrate recognition by replacing it with TK, GG, AA or LL...
December 22, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28011355/elimination-of-representative-contaminant-candidate-list-viruses-coxsackievirus-echovirus-hepatitis-a-virus-and-norovirus-from-water-by-coagulation-processes
#12
N Shirasaki, T Matsushita, Y Matsui, K Murai, A Aochi
We examined the removal of representative contaminant candidate list (CCL) viruses (coxsackievirus [CV] B5, echovirus type [EV] 11, and hepatitis A virus [HAV] IB), recombinant norovirus virus-like particles (rNV-VLPs), and murine norovirus (MNV) type 1 by coagulation. Water samples were subjected to coagulation with polyaluminum chloride (PACl, basicity 1.5) followed by either settling or settling and filtration. Together with our previously published results, the removal ratio order, as evaluated by a plaque-forming-unit method or an enzyme-linked immunosorbent assay after settling, was HAV>EV=rNV-VLPs≥CV=poliovirus type 1=MNV>adenovirus type 40 (range, 0...
December 8, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28011132/predominant-processing-adaptability-of-staphylococcus-xylosus-strains-isolated-from-chinese-traditional-low-salt-fermented-whole-fish
#13
Xuefeng Zeng, Laping He, Xu Guo, Li Deng, Wangen Yang, Qiujin Zhu, Zhenhua Duan
This study aimed to determine the predominant processing adaptability of 27 selected isolates of Staphylococcus xylosus in 'Suan yu', a traditional Chinese low-salt fermented whole-fish product. The isolates were screened for proteolytic, lipolytic, and enzymatic profiles; amino-acid decarboxylase content; antimicrobial activities; and tolerance to low temperatures, pH5.0, and salt. Two S. xylosus strains grew at 10°C in the presence of 10% NaCl and at pH5.0. Agar-plate assays and sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed that 21 and 8 of the strains exhibited appropriate proteolytic activities against myofibrillar and sarcoplasmic proteins, respectively...
February 2, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28002940/development-and-validation-of-a-qualitative-method-for-target-screening-of-448-pesticide-residues-in-fruits-and-vegetables-using-uhplc-esi-q-orbitrap-based-on-data-independent-acquisition-and-compound-database
#14
Jian Wang, Willis Chow, James Chang, Jon W Wong
A semiautomated qualitative method for target screening of 448 pesticide residues in fruits and vegetables was developed and validated using ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC/ESI Q-Orbitrap). The Q-Orbitrap Full MS/dd-MS(2) (data dependent acquisition) was used to acquire product-ion spectra of individual pesticides to build a compound database or an MS library, while its Full MS/DIA (data independent acquisition) was utilized for sample data acquisition from fruit and vegetable matrices fortified with pesticides at 10 and 100 μg/kg for target screening purpose...
January 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27998071/characterization-of-the-artemisinin-binding-site-for-translationally-controlled-tumor-protein-tctp-by-bioorthogonal-click-chemistry
#15
Weichao Li, Yiqing Zhou, Guanghui Tang, Youli Xiao
Despite the fact that multiple artemisinin-alkylated proteins in Plasmodium falciparum have been identified in recent studies, the alkylation mechanism and accurate binding site of artemisinin-protein interaction have remained elusive. Here, we report the chemical-probe-based enrichment of the artemisinin-binding peptide and characterization of the artemisinin-binding site of P. falciparum translationally controlled tumor protein (TCTP). A peptide fragment within the N-terminal region of TCTP was enriched and found to be alkylated by an artemisinin-derived probe...
December 21, 2016: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/27997398/toxicity-and-biofilm-based-selection-for-methyl-tert-butyl-ether-bioremediation-technology
#16
I M Guisado, J Purswani, L Catón-Alcubierre, J González-López, C Pozo
Extractive membrane biofilm reactor (EMBFR) technology offers productive solutions for volatile and semi-volatile compound removal from water bodies. In this study, the bacterial strains Paenibacillus etheri SH7(T) (CECT 8558), Agrobacterium sp. MS2 (CECT 8557) and Rhodococcus ruber strains A5 (CECT 8556), EE6 (CECT 8612) and EE1 (CECT 8555), previously isolated from fuel-contaminated sites, were tested for adherence on tubular semipermeable membranes in laboratory-scale systems designed for methyl tert-butyl ether (MTBE) bioremediation...
December 2016: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/27997138/analysis-of-hydroxyl-radicals-and-inactivation-mechanisms-of-bacteriophage-ms2-in-response-to-a-simultaneous-application-of-uv-and-chlorine
#17
Surapong Rattanakul, Kumiko Oguma
The simultaneous application of UV and chlorine (expressed as UV/Cl2) as a water treatment method may be a good disinfection option for UV-resistant microorganisms, such as human adenoviruses (HAdVs). In this study, we developed two approaches using UV/Cl2: one to quantitate the OH• radicals based on the degradation of the probe compound para-chlorobenzoic acid (pCBA) and the other to use bacteriophage MS2 to understand the virus inactivation mechanisms in response to UV, chlorine and UV/Cl2 disinfection using reverse-transcription quantitative polymerase chain reaction (RT-qPCR), attachment and genome penetration assays...
January 3, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/27993065/direct-and-indirect-photochemical-reactions-in-viral-rna-measured-with-rt-qpcr-and-mass-spectrometry
#18
Zhong Qiao, Krista R Wigginton
RNA carries the genetic instructions for many viruses to replicate in their host cells. The photochemical reactions that take place in RNA and affect viral infectivity in natural and engineered environments, however, remain poorly understood. We exposed RNA oligomer segments from the genome of bacteriophage MS2 to UV254, simulated sunlight, and singlet oxygen ((1)O2) and analyzed the oligomer reaction kinetics with reverse transcription quantitative PCR (RT-qPCR) and quantitative matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry (MS)...
December 20, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27992877/in-situ-structures-of-the-genome-and-genome-delivery-apparatus-in-a-single-stranded-rna-virus
#19
Xinghong Dai, Zhihai Li, Mason Lai, Sara Shu, Yushen Du, Z Hong Zhou, Ren Sun
Packaging of the genome into a protein capsid and its subsequent delivery into a host cell are two fundamental processes in the life cycle of a virus. Unlike double-stranded DNA viruses, which pump their genome into a preformed capsid, single-stranded RNA (ssRNA) viruses, such as bacteriophage MS2, co-assemble their capsid with the genome; however, the structural basis of this co-assembly is poorly understood. MS2 infects Escherichia coli via the host 'sex pilus' (F-pilus); it was the first fully sequenced organism and is a model system for studies of translational gene regulation, RNA-protein interactions, and RNA virus assembly...
December 19, 2016: Nature
https://www.readbyqxmd.com/read/27975475/hptlc-maldi-tof-ms-vs-hplc-esi-ms2-for-identification-to-flavonoids-in-plant-extracts
#20
L Fougère, D Da Silva, E Destandau, C Elfakir
No abstract text is available yet for this article.
December 2016: Planta Medica
keyword
keyword
5174
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"