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https://www.readbyqxmd.com/read/27917733/evaluation-of-an-ethanol-based-spray-disinfectant-for-decontamination-of-cover-gowns-prior-to-removal
#1
Sreelatha Koganti, Heba Alhmidi, Myreen E Tomas, Jennifer L Cadnum, Christopher Sass, Annette L Jencson, Curtis J Donskey
An ethanol-based spray disinfectant significantly reduced bacteriophage MS2 contamination on material from gowns meeting ASTM standard 1671 for resistance to blood and viral penetration and on a cover gown worn by personnel. Effectiveness of disinfection was affected by the type of gown material and the correctness of fit. Infect Control Hosp Epidemiol 2016;1-3.
December 5, 2016: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/27903464/a-peptide-retrieval-strategy-enables-significant-improvement-of-quantitative-performance-without-compromising-confidence-of-identification
#2
Chengjian Tu, Shichen Shen, Quanhu Sheng, Yu Shyr, Jun Qu
: Reliable quantification of low-abundance proteins in complex proteomes is challenging largely owing to the limited number of spectra/peptides identified. In this study we developed a straightforward method to improve the quantitative accuracy and precision of proteins by strategically retrieving the less confident peptides that were previously filtered out using the standard target-decoy search strategy. The filtered-out MS/MS spectra matched to confidently-identified proteins were recovered, and the peptide-spectrum-match FDR were re-calculated and controlled at a confident level of FDR≤1%, while protein FDR maintained at ~1%...
November 26, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27889622/evaluating-uv-c-led-disinfection-performance-and-investigating-potential-dual-wavelength-synergy
#3
Sara E Beck, Hodon Ryu, Laura A Boczek, Jennifer L Cashdollar, Kaitlyn M Jeanis, James S Rosenblum, Oliver R Lawal, Karl G Linden
A dual-wavelength UV-C LED unit, emitting at peaks of 260 nm, 280 nm, and the combination of 260|280 nm together was evaluated for its inactivation efficacy and energy efficiency at disinfecting Escherichia coli, MS2 coliphage, human adenovirus type 2 (HAdV2), and Bacillus pumilus spores, compared to conventional low-pressure and medium-pressure UV mercury vapor lamps. The dual-wavelength unit was also used to measure potential synergistic effects of multiple wavelengths on bacterial and viral inactivation and DNA and RNA damage...
November 7, 2016: Water Research
https://www.readbyqxmd.com/read/27889188/dynamic-margin-of-stability-during-gait-is-altered-in-persons-with-multiple-sclerosis
#4
Alexander T Peebles, Alyson Reinholdt, Adam P Bruetsch, Sharon G Lynch, Jessie M Huisinga
Persons with multiple sclerosis (PwMS) have high fall risk due to altered balance. To measure dynamic balance during walking, margin of stability (MoS) examines how the extrapolated center of mass moves relative to the base of support. This study investigates how MoS is affected in PwMS during walking at preferred, slow, and fast speeds, as well as the relationship between MoS and the Expanded Disability Severity Score (EDSS), fall history, and self-report balance confidence questionnaire. MoS was evaluated in PwMS without clinical gait impairment (MS1; n=20), PwMS with clinical gait impairment (MS2; n=20), and age-matched healthy controls (HC) (n=20), in the anterior/posterior (AP) and medial/lateral (ML) direction at heel strike and midstance...
November 10, 2016: Journal of Biomechanics
https://www.readbyqxmd.com/read/27876680/identification-of-unknown-rna-partners-using-maps
#5
David Lalaouna, Karine Prévost, Alex Eyraud, Eric Massé
Recent advances in high-throughput sequencing have led to an explosion in the rate of small regulatory RNAs (sRNAs) discovery among bacteria. However, only a handful of them are functionally characterized. Most of the time, little to no targets are known. In Lalaouna et al. (2015), we proposed a new technology to uncover sRNAs targetome, which is based on the MS2-affinity purification (MAPS). We were able to prove its efficiency by applying it on well-characterized sRNAs of Escherichia coli. Thereafter, we adapted the procedure to other kind of RNA (mRNAs and tRNA-derived RNA fragments) and bacteria (pathogenic or Gram-positive strains)...
November 19, 2016: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/27873218/characterization-of-glycan-structures-of-chondroitin-sulfate-glycopeptides-facilitated-by-sodium-ion-pairing-and-positive-mode-lc-ms-ms
#6
Jonas Nilsson, Fredrik Noborn, Alejandro Gomez Toledo, Waqas Nasir, Carina Sihlbom, Göran Larson
Purification and liquid chromatography-tandem mass spectrometry (LC-MS/MS) characterization of glycopeptides, originating from protease digests of glycoproteins, enables site-specific analysis of protein N- and O-glycosylations. We have described a protocol to enrich, hydrolyze by chondroitinase ABC, and characterize chondroitin sulfate-containing glycopeptides (CS-glycopeptides) using positive mode LC-MS/MS. The CS-glycopeptides, originating from the Bikunin proteoglycan of human urine samples, had ΔHexAGalNAcGlcAGalGalXyl-O-Ser hexasaccharide structure and were further substituted with 0-3 sulfate and 0-1 phosphate groups...
November 21, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27867703/deep-imaging-of-absorption-and-scattering-features-by-multispectral-multiple-scattering-low-coherence-interferometry
#7
Yang Zhao, Jason R Maher, Mohamed M Ibrahim, Jennifer S Chien, Howard Levinson, Adam Wax
We have developed frequency domain multispectral multiple scattering low coherence interferometry (ms2/LCI) for deep imaging of absorption and scattering contrast. Using tissue-mimicking phantoms that match the full scattering phase function of human dermal tissue, we demonstrate that ms2/LCI can provide a signal/noise ratio (SNR) improvement of 15.4 dB over conventional OCT at an imaging depth of 1 mm. The enhanced SNR and penetration depth provided by ms2/LCI could be leveraged for a variety of clinical applications including the assessment of burn injuries where current clinical classification of severity only provides limited accuracy...
October 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27836658/comparison-of-rna-extraction-kits-for-the-purification-and-detection-of-an-enteric-virus-surrogate-on-green-onions-via-rt-pcr
#8
Ruoyang Xu, Y Carol Shieh, Diana S Stewart
Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) offers a rapid and sensitive molecular method for detection of enteric viruses. Unfortunately, these assays are often hampered by the low virus titre found in foods and PCR inhibition due to matrix carryover during RNA extraction. Four commercial RNA extraction kits (Qiagen's QIAamp Viral RNA Mini and UltraSens Virus kits, MoBio UltraClean Tissue & Cells RNA Isolation kit, and Ambion MagMAX Viral RNA Isolation kit) were evaluated for their ability to extract and purify MS2 bacteriophage RNA, an enteric virus surrogate, from inoculated green onions, a food which has been associated with viral gastroenteritis outbreaks...
January 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/27815782/preliminary-source-tracking-of-male-specific-f-rna-coliphage-on-lettuce-as-a-surrogate-of-enteric-viruses-using-reverse-transcription-pcr
#9
Mojgan Yazdi, Masoud Yavarmanesh, Masumeh Bahreini, Mohebbat Mohebbi
The aim of this research was to preliminary track fecal source male-specific F(+)RNA coliphages including human and animals in lettuce. At first, two published virus extraction procedures of ultracentrifugation and PEG precipitation were compared using DAL assay for determining the recovery efficiency in lettuce spiked artificially with three concentrations (10(2), 10(4), 10(6) pfu/100 ml) of MS2 coliphage. The results showed that PEG precipitation had the highest recovery in which the recovery efficiency at the spiked level of 10(6) pfu/100 ml was 16...
November 4, 2016: Food and Environmental Virology
https://www.readbyqxmd.com/read/27806443/application-of-the-half-decimal-place-rule-to-increase-the-peptide-identification-rate
#10
Christian J Koehler, Ravi Chand Bollineni, Bernd Thiede
RATIONALE: Many MS2 spectra in bottom-up proteomics experiments remain unassigned. To improve proteome coverage, we applied the half decimal place rule (HDPR) to remove non-peptidic molecules. The HDPR considers the ratio of the digits after the decimal point to the full molecular mass and results in a relatively small permitted mass window for most peptides. METHODS: First, the HDPR mass filter was calculated for the human and other proteomes. Subsequently, the HDPR was applied to three technical replicates of an in-solution tryptic digest of HeLa cells which were analysed by LC-MS using a quadrupole-orbitrap mass spectrometer (Q Exactive)...
November 2, 2016: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/27805355/purification-and-quantification-of-an-isomeric-compound-in-a-mixture-by-collisional-excitation-in-multistage-mass-spectrometry-experiments
#11
Dany Jeanne Dit Fouque, Alicia Maroto, Antony Memboeuf
The differentiation, characterization, and quantification of isomers and/or isobars in mixtures is a recurrent problem in mass spectrometry and more generally in analytical chemistry. Here we present a new strategy to assess the purity of a compound that is susceptible to be contaminated with another isomeric side-product in trace levels. Providing one of the isomers is available as pure sample, this new strategy allows the detection of isomeric contamination. This is done thanks to a "gas-phase collisional purification" inside an ion trap mass spectrometer paving the way for an improved analysis of at least similar samples...
November 3, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27798678/combining-genes-from-multiple-phages-for-improved-cell-lysis-and-dna-transfer-from-escherichia-coli-to-bacillus-subtilis
#12
Mario Juhas, Christine Wong, James W Ajioka
The ability to efficiently and reliably transfer genetic circuits between the key synthetic biology chassis, such as Escherichia coli and Bacillus subtilis, constitutes one of the major hurdles of the rational genome engineering. Using lambda Red recombineering we integrated the thermosensitive lambda repressor and the lysis genes of several bacteriophages into the E. coli chromosome. The lysis of the engineered autolytic cells is inducible by a simple temperature shift. We improved the lysis efficiency by introducing different combinations of lysis genes from bacteriophages lambda, ΦX174 and MS2 under the control of the thermosensitive lambda repressor into the E...
2016: PloS One
https://www.readbyqxmd.com/read/27798611/directed-evolution-using-dcas9-targeted-somatic-hypermutation-in-mammalian-cells
#13
Gaelen T Hess, Laure Frésard, Kyuho Han, Cameron H Lee, Amy Li, Karlene A Cimprich, Stephen B Montgomery, Michael C Bassik
Engineering and study of protein function by directed evolution has been limited by the technical requirement to use global mutagenesis or introduce DNA libraries. Here, we develop CRISPR-X, a strategy to repurpose the somatic hypermutation machinery for protein engineering in situ. Using catalytically inactive dCas9 to recruit variants of cytidine deaminase (AID) with MS2-modified sgRNAs, we can specifically mutagenize endogenous targets with limited off-target damage. This generates diverse libraries of localized point mutations and can target multiple genomic locations simultaneously...
October 31, 2016: Nature Methods
https://www.readbyqxmd.com/read/27796786/is-photoisomerization-required-for-no-photorelease-in-ruthenium-nitrosyl-complexes
#14
Juan Sanz García, Fabienne Alary, Martial Boggio-Pasqua, Isabelle M Dixon, Jean-Louis Heully
The factors that explain the competition between intramolecular NO linkage photoisomerization and NO photorelease in five ruthenium nitrosyl complexes were investigated. By applying DFT-based methods, it was possible to characterize the ground states and lowest triplet potential energy surfaces of these species, and to establish that both photoisomerization and photorelease processes can occur in the lowest triplet state of each species. This work highlights the crucial role of the sideways-bonded isomer, a metastable state also known as the MS2 isomer, in the photochemical loss of NO, while the results obtained also indicate that the population of the triplet state of this isomer is compulsory for both processes and show how photoisomerization and photorelease interfere...
November 2016: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/27794587/phenolic-compounds-of-potato-peel-extracts-their-antioxidant-activity-and-protection-against-human-enteric-viruses
#15
Norma Patricia Silva-Beltrán, Cristóbal Chaidez-Quiroz, Osvaldo López-Cuevas, Saul Ruiz-Cruz, Marco A López-Mata, Carmen Lizette Del-Toro-Sánchez, Enrique Marquez-Rios, José de Jesús Ornelas-Paz
Potato peels (PP) contain several bioactive compounds. These compounds are known to provide human health benefits including antioxidant and antimicrobial properties. In addition, these compounds could have effects on human enteric viruses that have not yet been reported. The objective of the present study was to evaluate the phenolic composition, antioxidant properties in the acidified ethanol extract (AEE) and water extract (WE) of PP, and the antiviral effects on the inhibition of Av-05 and MS2 bacteriophages, which were used as human enteric viral surrogates...
October 28, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27794229/corrigendum-to-investigation-of-enteric-adenovirus-and-poliovirus-removal-by-coagulation-processes-and-suitability-of-bacteriophages-ms2-and-%C3%AF-x174-as-surrogates-for-those-viruses-sci-total-environ-563-564-year-29-39
#16
N Shirasaki, T Matsushita, Y Matsui, T Marubayashi, K Murai
No abstract text is available yet for this article.
October 26, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27788152/impact-of-diabetes-type-1-in-children-on-autonomic-modulation-at-rest-and-in-response-to-the-active-orthostatic-test
#17
Thais Roque Giacon, Franciele Marques Vanderlei, Diego Giulliano Destro Christofaro, Luiz Carlos Marques Vanderlei
INTRODUCTION: Cardiovascular autonomic neuropathy is one of the most common complications of diabetes mellitus type 1 (DM1), of which one of the first subclinical manifestations is changes in heart rate variability (HRV). Thus, analysis of HRV associated with the autonomic active orthostatic test is important in this population. OBJECTIVES: To analyze the autonomic modulation responses induced by the implementation of the active orthostatic test, in children with DM1, and study the autonomic modulation by means of HRV indices...
2016: PloS One
https://www.readbyqxmd.com/read/27786464/gentle-sampling-of-submicrometer-airborne-virus-particles-using-a-personal-electrostatic-particle-concentrator
#18
Seongkyeol Hong, Jyoti Bhardwaj, Chang-Ho Han, Jaesung Jang
Measurements of airborne viruses via sampling have been critical issues. Most electrostatic samplers have been assessed for bacterial aerosols or micrometer-sized viral particles; however, sampling of submicrometer-sized airborne viruses is necessary, especially because of the high probability of their staying airborne and their deposition in the lower respiratory tract. Here, we present a novel personal electrostatic particle concentrator (EPC) for gentle sampling of submicrometer airborne virus particles...
October 27, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27783334/inactivation-of-viruses-and-bacteriophages-as-models-for-swine-hepatitis-e-virus-in-food-matrices
#19
Eva Emmoth, Jordi Rovira, Andreja Rajkovic, Elena Corcuera, Diego Wilches Pérez, Irene Dergel, Jakob R Ottoson, Frederik Widén
Hepatitis E virus has been recognised as a food-borne virus hazard in pork products, due to its zoonotic properties. This risk can be reduced by adequate treatment of the food to inactivate food-borne viruses. We used a spectrum of viruses and bacteriophages to evaluate the effect of three food treatments: high pressure processing (HPP), lactic acid (LA) and intense light pulse (ILP) treatments. On swine liver at 400 MPa for 10 min, HPP gave log10 reductions of ≥4.2, ≥5.0 and 3.4 for feline calicivirus (FCV) 2280, FCV wildtype (wt) and murine norovirus 1 (MNV 1), respectively...
October 25, 2016: Food and Environmental Virology
https://www.readbyqxmd.com/read/27777761/a-pilot-study-to-assess-use-of-fluorescent-lotion-in-patient-care-simulations-to-illustrate-pathogen-dissemination-and-train-personnel-in-correct-use-of-personal-protective-equipment
#20
Heba Alhmidi, Sreelatha Koganti, Myreen E Tomas, Jennifer L Cadnum, Annette Jencson, Curtis J Donskey
BACKGROUND: Simulations using fluorescent tracers can be useful in understanding the spread of pathogens and in devising effective infection control strategies. METHODS: During simulated patient care interactions in which providers wore gloves and gowns, we evaluated environmental and personnel dissemination of fluorescent lotion and bacteriophage MS2 from a contaminated mannequin. The frequency of skin and clothing contamination after removal of personal protective equipment (PPE) was compared before versus after an intervention that included education and practice in PPE donning and doffing...
2016: Antimicrobial Resistance and Infection Control
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