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https://www.readbyqxmd.com/read/29245156/selection-and-targeting-of-epcam-protein-by-ssdna-aptamer
#1
Walhan Alshaer, Nida Ababneh, Mamon Hatmal, Heba Izmirli, Moujab Choukeife, Alaa Shraim, Nour Sharar, Aya Abu-Shiekah, Fadwa Odeh, Abeer Al Bawab, Abdalla Awidi, Said Ismail
Aptamers are molecules that reveal highly complex and refined molecular recognition properties. These molecules are capable of binding with high affinity and selectivity to targets, ranging from small molecules to whole living cells. Several aptamers have been selected for targeting cellular proteins and they have also used in developing therapeutics and diagnostic strategies. Epithelial cell adhesion molecule (EpCAM) is considered as a cancer stem cell (CSC) biomarker and one of the most promising targets for aptamer selection against CSCs...
2017: PloS One
https://www.readbyqxmd.com/read/29245100/student-motivation-stressors-and-intent-to-leave-nursing-doctoral-study-a-national-study-using-path-analysis
#2
Delene Volkert, Lori Candela, Matthew Bernacki
BACKGROUND: The demand for doctorally prepared nurses worldwide is higher than ever. Universities have responded with increased numbers of DNP and Ph.D. in Nursing programs. There are more doctoral nursing students than ever before yet they remain one of the least studied student populations. This is concerning given the high attrition rates reported in doctoral programs. The few studies that do exist are typically qualitative and exploratory in nature. OBJECTIVES: The aim of this national study of Ph...
December 6, 2017: Nurse Education Today
https://www.readbyqxmd.com/read/29245071/dual-task-training-in-older-adults-the-effect-of-additional-motor-tasks-on-mobility-performance
#3
Paolo Riccardo Brustio, Emanuela Rabaglietti, Sabrina Formica, Monica Emma Liubicich
OBJECTIVES: Dual-task (DT) performance is common to most activities of daily living and difficulties in DT activities may reduce quality of life in older adults. This study investigated the effect of DT training in a sample of older adults. METHODS: Sixty older adults (mean=74.4±3.1years) participated in the study. Twenty-two older adults were included in the control (CG), 19 in the single-task (ST) training and 19 in DT training group. ST group received balance and walking training twice a week for16 weeks, while DT training group performed the same training with additional motor tasks...
December 10, 2017: Archives of Gerontology and Geriatrics
https://www.readbyqxmd.com/read/29245063/background-levels-of-male-dna-in-the-vaginal-cavity
#4
Patricia P Albani, Jayshree Patel, Rachel I Fleming
In alleged sexual assault investigations, the detection of male DNA on female intimate swabs can be established through Y-STR profiling. However, in cases where the defendant lives in the same dwelling as the complainant, the defendant may argue that his DNA was transferred through normal social contact, and dispute that intimate contact occurred. This study aimed to determine whether or not male DNA could be detected in samples collected from the vaginal cavity after everyday activities. A total of 300 samples from eight individuals were subjected to DNA quantification and Y-STR amplification...
December 6, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29245037/production-of-bio-fertilizer-from-microwave-vacuum-pyrolysis-of-palm-kernel-shell-for-cultivation-of-oyster-mushroom-pleurotus-ostreatus
#5
Wai Lun Nam, Xue Yee Phang, Man Huan Su, Rock Keey Liew, Nyuk Ling Ma, Mohd Haqqi Nazilli Bin Rosli, Su Shiung Lam
Microwave vacuum pyrolysis of palm kernel shell (PKS) was performed to produce biochar, which was then tested as bio-fertilizer in growing Oyster mushroom (Pleurotus ostreatus). The pyrolysis approach produced biochar containing a highly porous structure with a high BET surface area of up to 270m2/g and low moisture content (≤10wt%), exhibiting desirable adsorption properties to be used as bio-fertilizer since it can act as a housing that provides many sites on which living microorganisms (mycelium or plant-growth promoting bacteria) and organic nutrients can be attached or adsorbed onto...
December 12, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29244821/genotyping-of-mycobacterium-leprae-for-better-understanding-of-leprosy-transmission-in-fortaleza-northeastern-brazil
#6
Amanda N B Fontes, Luana N G C Lima, Rosa M S Mota, Rosa L F Almeida, Maria A Pontes, Heitor de S Gonçalves, Cristiane C Frota, Varalakshmi D Vissa, Patrick J Brennan, Ricardo J P S Guimaraes, Carl Kendall, Ligia R F S Kerr, Philip N Suffys
Leprosy is endemic in large part of Brazil with 28,761 new patients in 2015, the second largest number worldwide and reaches 9/10.000 in highly endemic regions and 2.7/10.000 in the city of Fortaleza, Ceará, Northeast Brazil. For better understanding of risk factors for leprosy transmission, we conducted an epidemiologic study supplemented by 17 locus VNTR and SNP 1-4 typing of Mycobacterium leprae in skin biopsy samples from new multibacillary (MB) patients diagnosed at a reference center in 2009 and 2010...
December 15, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29244760/impact-of-methods-on-the-measurement-of-mrna-turnover
#7
REVIEW
Takeo Wada, Attila Becskei
The turnover of the RNA molecules is determined by the rates of transcription and RNA degradation. Several methods have been developed to study RNA turnover since the beginnings of molecular biology. Here we summarize the main methods to measure RNA half-life: transcription inhibition, gene control, and metabolic labelling. These methods were used to detect the cellular activity of the mRNAs degradation machinery, including the exo-ribonuclease Xrn1 and the exosome. On the other hand, the study of the differential stability of mature RNAs has been hampered by the fact that different methods have often yielded inconsistent results...
December 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29244744/a-carbon-dioxide-limitation-inducible-protein-cola-supports-the-growth-of-synechococcus-sp-pcc-7002
#8
Ginga Shimakawa, Satoru Watanabe, Chikahiro Miyake
A limitation in carbon dioxide (CO₂), which occurs as a result of natural environmental variation, suppresses photosynthesis and has the potential to cause photo-oxidative damage to photosynthetic cells. Oxygenic phototrophs have strategies to alleviate photo-oxidative damage to allow life in present atmospheric CO₂ conditions. However, the mechanisms for CO₂ limitation acclimation are diverse among the various oxygenic phototrophs, and many mechanisms remain to be discovered. In this study, we found that the gene encoding a CO₂ limitation-inducible protein, ColA, is required for the cyanobacterium Synechococcus sp...
December 15, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29244734/-i-learned-to-let-go-of-my-pain-the-effects-of-mindfulness-meditation-on-adolescents-with-chronic-pain-an-analysis-of-participants-treatment-experience
#9
Danielle Ruskin, Lauren Harris, Jennifer Stinson, Sara Ahola Kohut, Katie Walker, Erinn McCarthy
Chronic pain can lead to significant negative outcomes across many areas of life. Recently, mindfulness-based interventions (MBIs) have been identified as potentially effective tools for improved pain management among adolescents living with pain. This study aimed to explore the experience of adolescents who participated in an eight-week mindfulness group adapted for adolescents with chronic pain (MBI-A), and obtain their feedback and suggestions on group structure and content. A mixed method design was used employing qualitative data from focus groups and data from a satisfaction questionnaire...
December 15, 2017: Children
https://www.readbyqxmd.com/read/29244728/transcriptome-analysis-of-paraburkholderia-phymatum-under-nitrogen-starvation-and-during-symbiosis-with-phaseolus-vulgaris
#10
Martina Lardi, Yilei Liu, Gabriela Purtschert, Samanta Bolzan de Campos, Gabriella Pessi
Paraburkholderia phymatum belongs to the β-subclass of proteobacteria. It has recently been shown to be able to nodulate and fix nitrogen in symbiosis with several mimosoid and papilionoid legumes. In contrast to the symbiosis of legumes with α-proteobacteria, very little is known about the molecular determinants underlying the successful establishment of this mutualistic relationship with β-proteobacteria. In this study, we performed an RNA-sequencing (RNA-seq) analysis of free-living P. phymatum growing under nitrogen-replete and -limited conditions, the latter partially mimicking the situation in nitrogen-deprived soils...
December 15, 2017: Genes
https://www.readbyqxmd.com/read/29244696/selective-spinal-fusion-for-neuromuscular-scoliosis-in-a-patient-with-pompe-disease-a-case-report-and-review-of-the-literature
#11
Atsushi Tanida, Shinji Tanishima, Tokumitsu Mihara, Aya Narita, Yoshihiro Maegaki, Hideki Nagashima
CASE: A 16-year-old girl with Pompe disease underwent surgery for scoliosis. She had been able to walk without any assistance, and kept her balance by swinging her waist. Therefore, we performed posterior selective spinal correction and fusion to avoid any adverse effects on walking ability that could occur with immobilization of the lumbosacral spine. After surgery, she was highly satisfied with her ability to perform the activities of daily living. CONCLUSION: For nonambulatory patients with scoliosis and Pompe disease, long fusion from the upper thoracic spine to the pelvis is generally required...
January 2017: JBJS Case Connector
https://www.readbyqxmd.com/read/29244653/high-capacity-lithium-ion-batteries-composed-of-cobalt-oxide-nanoparticle-anodes-and-raman-spectroscopic-analysis-of-nanoparticle-strain-dynamics-in-batteries
#12
Mohammad Islam, Mateusz Zuba, Vincent Debiase, Nicholas Noviasky, Christopher Hawley
Cobalt nanoparticle thin films were electrophoretically deposited on copper current collectors and were annealed into thin films of hollow Co3O4 nanoparticles. These thin films were directly used as the anodes of lithium ion batteries without the addition of conducting carbons and bonding agents. Lithium ion batteries thus fabricated show high gravimetric capacities and long cycle lives. For 1.0 μm thick Co3O4 nanoparticle films the gravimetric capacities of the batteries were more than 800 mAh/g at a current rate of C/15 which is about 90% of the theoretical maximum...
December 15, 2017: Nanotechnology
https://www.readbyqxmd.com/read/29244485/critical-role-of-trp-588-of-presynaptic-munc13-1-for-ligand-binding-and-membrane-translocation
#13
Joydip Das, Noemi Kedei, Jessica S Kelsey, Youngki You, Satyabrata Pany, Gary A Mitchell, Nancy E Lewin, Peter M Blumberg
Munc13-1 is a presynaptic active-zone protein essential for neurotransmitter release and presynaptic plasticity in the brain. This multi-domain scaffold protein contains a C1 domain that binds to the activator diacylglycerol/phorbol ester. Although the C1 domain bears close structural homology with the C1 domains of protein kinase C (PKC), the tryptophan residue at position 22 (588 in the full-length Munc13-1) occludes the activator binding pocket, which is not the case for PKC. To elucidate the role of this tryptophan, we generated W22A, W22K, W22D, W22Y and W22F substitutions in the full-length Munc13-1, expressed the GFP- tagged constructs in Neuro-2a cells, and measured their membrane translocation in response to phorbol ester treatment by imaging of the live cells using confocal microscopy...
December 15, 2017: Biochemistry
https://www.readbyqxmd.com/read/29244234/mechanisms-of-caspr2-antibodies-in-autoimmune-encephalitis-and-neuromyotonia
#14
Kristina R Patterson, Josep Dalmau, Eric Lancaster
OBJECTIVE: To determine the pathogenic mechanisms of autoantibodies to the cell adhesion molecule Caspr2 in acquired neuromyotonia and autoimmune encephalitis. METHODS: Caspr2 positive samples were confirmed using a cell-based assay, and their IgG subtypes were determined by ELISA and cell-based assay. A solid phase binding assay quantified the binding of Caspr2 to contactin-2 in the presence of Caspr2 autoantibodies. Living cultures of primary rat hippocampal neurons were incubated with Caspr2-positive or control sera and the distribution of Caspr2-positive immunofluorescent puncta and total surface Caspr2 were quantified...
December 15, 2017: Annals of Neurology
https://www.readbyqxmd.com/read/29244172/human-in-vitro-spermatogenesis-from-pluripotent-stem-cells-in-need-of-a-stepwise-differentiation-protocol
#15
C Rombaut, H Mertes, B Heindryckx, E Goossens
Recently, complete in-vitro generation of male gametes starting from pluripotent stem cells was obtained in a mouse model with live offspring as a result. This breakthrough was probably due to the use of a stepwise differentiation protocol taking the tightly regulated in-vivo situation into account. As shown previously, factors of the TGFβ superfamily, metabolites of vitamin A, growth hormones, sex steroids and, most importantly, somatic cell support are major regulators of the development, survival, proliferation and differentiation of male gametes...
December 13, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29244163/regulation-of-chromosome-segregation-in-oocytes-and-the-cellular-basis-for-female-meiotic-errors
#16
Jessica Greaney, Zhe Wei, Hayden Homer
BACKGROUND: Meiotic chromosome segregation in human oocytes is notoriously error-prone, especially with ageing. Such errors markedly reduce the reproductive chances of increasing numbers of women embarking on pregnancy later in life. However, understanding the basis for these errors is hampered by limited access to human oocytes. OBJECTIVE AND RATIONALE: Important new discoveries have arisen from molecular analyses of human female recombination and aneuploidy along with high-resolution analyses of human oocyte maturation and mouse models...
December 13, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/29244155/differential-rpa-1-and-rad-51-recruitment-in-vivo-throughout-the-c-elegans-germline-as-revealed-by-laser-microirradiation
#17
Emily Koury, Kailey Harrell, Sarit Smolikove
Studies of the repair pathways associated with DNA double strand breaks (DSBs) are numerous, and provide evidence for cell-cycle specific regulation of homologous recombination (HR) by the regulation of its associated proteins. Laser microirradiation is a well-established method to examine in vitro kinetics of repair and allows for live-imaging of DSB repair from the moment of induction. Here we apply this method to whole, live organisms, introducing an effective system to analyze exogenous, microirradiation-induced breaks in the Caenorhabditis elegans germline...
December 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29244142/an-autofluorescence-based-method-for-the-isolation-of-highly-purified-ventricular-cardiomyocytes
#18
Veronica Larcher, Paolo Kunderfranco, Marco Vacchiano, Pierluigi Carullo, Marco Erreni, Irene Salamon, Federico Simone Colombo, Enrico Lugli, Marta Mazzola, Achille Anselmo, Gianluigi Condorelli
Aims: The aim of our study was to set up a simple and reliable isolation method of living ventricular cardiomyocytes (vCMs) for molecular and biological studies. Methods: A standard technique for the retrograde perfusion of an enzymatic solution was used to isolate cardiac cells from adult mouse heart. Fluorescence-activated cell sorting (FACS) on adult murine cardiac ventricle cells was performed, comparing the intrinsic autofluorescence in the FITC channel and the forward scatter (FSC) parameter in order to isolate highly fluorescent cells...
December 13, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/29244126/silent-cerebral-small-vessel-disease-twice-as-prevalent-in-middle-aged-well-controlled-cart-treated-hiv-infected-individuals-than-hiv-uninfected-individuals
#19
Antoine Moulignier, Julien Savatovsky, Lambert Assoumou, François-Xavier Lescure, Cédric Lamirel, Ophelia Godin, Nadia Valin, Roland Tubiana, Ana Canestri, Pascal Roux, Jean-Claude Sadik, Laurence Salomon, Marie Abrivard, Christine Katlama, Yazdan Yazdanpanah, Gilles Pialoux, Pierre-Marie Girard, Dominique Costagliola
Background: Silent cerebral small-vessel disease (CSVD) is defined as white matter hyperintensities, silent brain infarction or microbleeds. CSVD is responsible for future vascular events, cognitive impairment, frailty and shorter survival. CSVD prevalence among middle-aged persons living with well-controlled HIV infection (PLHIVs) is unknown. Methods: ANRS EP51 MicroBREAK (NCT02082574) is a cross-sectional study with prospective enrollment of treated PLHIVs, ≥50 years old with viral load controlled for ≥12 months, and frequency age- and sex-matched HIV-uninfected controls (HUCs)...
December 13, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29244105/the-gendered-health-workforce-mixed-methods-analysis-from-four-fragile-and-post-conflict-contexts
#20
Sophie Witter, Justine Namakula, Haja Wurie, Yotamu Chirwa, Sovanarith So, Sreytouch Vong, Bandeth Ros, Stephen Buzuzi, Sally Theobald
It is well known that the health workforce composition is influenced by gender relations. However, little research has been done which examines the experiences of health workers through a gender lens, especially in fragile and post-conflict states. In these contexts, there may not only be opportunities to (re)shape occupational norms and responsibilities in the light of challenges in the health workforce, but also threats that put pressure on resources and undermine gender balance, diversity and gender responsive human resources for health (HRH)...
December 1, 2017: Health Policy and Planning
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