keyword
MENU ▼
Read by QxMD icon Read
search

Corneal Transplant

keyword
https://www.readbyqxmd.com/read/29027064/light-scattering-measurements-in-electron-beam-sterilized-corneas-stored-in-recombinant-human-serum-albumin
#1
Khoa D Tran, Yan Li, Jeffrey D Holiman, Maolong Tang, David Huang, Michael D Straiko, Christopher G Stoeger
Irradiated corneal tissues have been used for a variety of ophthalmic procedures including glaucoma drainage device covers and lamellar grafts. The maintenance of corneal clarity is important, as light obstructions resulting from processing or long-term storage of irradiated corneas may negatively affect vision and postoperative cosmesis. It has been reported that corneal tissues can be preserved in human serum albumin (HSA), however, the clarity of corneas after long-term storage in HSA has not been well described...
October 12, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29023237/corneal-tissue-from-dry-eye-donors-leads-to-enhanced-graft-rejection
#2
Takenori Inomata, Jing Hua, Takeshi Nakao, Tina Shiang, Homer Chiang, Afsaneh Amouzegar, Reza Dana
PURPOSE: To assess the effect of dry eye disease (DED) in graft donors on dendritic cell (DC) maturation, host T-cell sensitization, and corneal allograft rejection. METHODS: Corneas of control (healthy donor) and DED mice (C57BL/6) were transplanted onto fully allogeneic naive BALB/c recipients (n = 10 mice/group). Long-term allograft survival was evaluated for 8 weeks. Corneas and draining lymph nodes (dLNs) were harvested at posttransplantation day 14 (n = 5 mice/group)...
October 10, 2017: Cornea
https://www.readbyqxmd.com/read/29021411/biomaterials-for-corneal-bioengineering
#3
Zhi Chen, Jingjing You, Xiao Liu, Simon Cooper, Christopher Hodge, Gerard Sutton, Jeremy Micah Crook, Gordon G Wallace
Corneal transplantation is an important surgical treatment for many common corneal diseases. However, a worldwide shortage of tissue from suitable corneal donors has meant that many people are not able to receive sight-restoring operations. In addition, rejection is a major cause of corneal transplant failure. Bioengineering corneal tissue has recently gained widespread attention. In order to facilitate corneal regeneration, a range of materials is currently being investigated. The ideal substrate requires sufficient tectonic durability, biocompatibility with cultured cellular elements, transparency, and perhaps biodegradability and clinical-compliance...
October 12, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28991852/preparation-and-biomechanical-properties-of-an-acellular-porcine-corneal-stroma
#4
Qing Li, Hongmei Wang, Zhenye Dai, Yichen Cao, Chuanyu Jin
PURPOSE: To construct an acellular porcine corneal stroma (aPCS) as a human corneal stroma alternative and to further explore its biomechanical properties. METHODS: A combination of DNA-RNA enzymes and ultrasound technology was used to strip the native porcine corneal cells. The microstructure of aPCS was observed by H&E staining, DAPI staining, and α-Gal tests. The mechanical properties were detected by a tension machine. Cytotoxicity of aPCS was measured by the MTT assay...
November 2017: Cornea
https://www.readbyqxmd.com/read/28990995/donor-tissue-preparation-for-descemet-membrane-endothelial-keratoplasty-an-updated-review
#5
Rénuka S Birbal, Shameema Sikder, Jessica T Lie, Esther A Groeneveld-van Beek, Silke Oellerich, Gerrit R J Melles
PURPOSE: To provide an overview of the current literature on donor tissue preparation for Descemet membrane endothelial keratoplasty (DMEK). METHODS: A comprehensive database search without date restrictions was performed in PubMed and in The Cochrane Library in May, 2017. Keywords included Descemet membrane endothelial keratoplasty, corneal transplantation, graft, harvest, dissection, preparation, endothelial cell, and endothelial cell density. Articles aiming to describe or evaluate a technique for DMEK graft preparation were considered eligible and were included in this review...
October 5, 2017: Cornea
https://www.readbyqxmd.com/read/28990994/long-term-visual-outcomes-complications-and-retention-of-the-boston-type-i-keratoprosthesis
#6
Carolina Aravena, Fei Yu, Anthony J Aldave
PURPOSE: To report the long-term outcomes of the Boston type I keratoprosthesis (KPro). METHODS: Data were collected regarding preoperative characteristics, surgical procedure(s) performed, and postoperative outcomes of patients who underwent KPro implantation from May 1, 2004, to May 1, 2011, and were followed for 5 years after surgery. RESULTS: Seventy-four KPro procedures were performed in 58 eyes (55 patients), with more than 1 KPro implanted in 10 eyes...
October 5, 2017: Cornea
https://www.readbyqxmd.com/read/28990426/gene-therapy-for-modulation-of-t-cell-mediated-immune-response-provoked-by-corneal-transplantation
#7
Marko Pastak, Veronika Kleff, Daniel Raphael Saban, Marta Czugala, Klaus-Peter Steuhl, Süleyman Ergün, Bernhard Singer, Thomas Armin Fuchsluger
Corneal transplantation (=keratoplasty) is the most common type of tissue replacement in the world. The increased rate of graft rejection after keratoplasty is a central problem for repeated transplantations and in inflamed host corneas. It has been shown that apoptosis of grafted epithelium has a role in corneal allograft rejection. Our study focused on the T cell response triggered in BALB/c mice after allogeneic corneal transplantation with and without anti-apoptotic p35-transduced epithelium. To restrict p35 expression to the epithelial cells we created modified allogeneic composite grafts...
October 7, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/28983455/long-term-cognitive-and-somatic-outcomes-of-enzyme-replacement-therapy-in-untransplanted-hurler-syndrome
#8
Julie B Eisengart, Jeanine Jarnes, Alia Ahmed, Igor Nestrasil, Richard Ziegler, Kathleen Delaney, Elsa Shapiro, Chester Whitley
Mucopolysaccharidosis type I (MPS I) was added to the Recommended Uniform Screening Panel for newborn screening in 2016, highlighting recognition that early treatment of MPS I is critical to stem progressive, irreversible disease manifestations. Enzyme replacement therapy (ERT) is an approved treatment for all MPS I phenotypes, but because the severe form (MPS IH, Hurler syndrome) involves rapid neurocognitive decline, the impermeable blood-brain-barrier is considered an obstacle for ERT. Instead, hematopoietic cell transplantation (HCT) has long been recommended, as it is believed to be the only therapy that arrests neurocognitive decline...
December 2017: Molecular Genetics and Metabolism Reports
https://www.readbyqxmd.com/read/28982949/the-existence-of-dead-cells-in-donor-corneal-endothelium-preserved-with-storage-media
#9
Koji Kitazawa, Tsutomu Inatomi, Hidetoshi Tanioka, Satoshi Kawasaki, Hiroko Nakagawa, Osamu Hieda, Hideki Fukuoka, Naoki Okumura, Noriko Koizumi, Bernie Iliakis, Chie Sotozono, Shigeru Kinoshita
AIM: To investigate the viability of donor corneal endothelial cells (CECs) preserved in storage media by histological examination. METHODS: Twenty-eight donor corneas were obtained from SightLife Eye Bank (Seattle, Washington), and redundant peripheral portions of those corneas were used for histological examination after removal of the centre corneal graft for transplantation. To assess cell viability in the corneal endothelium, biostaining experiments were performed using propidium iodide, calcein-AM, Hoechst 33 342, annexin V, anti-vimentin antibody and toluidine blue...
October 5, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28980569/-severe-bilateral-pseudomonas-sclerokeratitis-in-comatose-patient-clinical-case
#10
Evg A Kasparova, A A Kasparov, E A Kasparova, A V Zaytsev
The article presents a clinical case of severe bilateral pseudomonas sclerokeratitis in a patient with occlusion hydrocephalus and intracranial hypertension, who was in a coma and on a ventilator for 20 days. At first examination (7 days after the onset of purulent keratitis, during which the process had been rapidly progressing), the clinical picture included lagophthalmos, severe purulent corneal ulcer, bilateral purulent scleromalacia, perforated cornea in the left eye. On the same day, in order to maintain eye integrity, urgent reconstructive penetrating sclerokeratoplasty with subsequent sclerocorneal coating was performed in both eyes right in the intensive care unit...
2017: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/28979685/the-injection-of-discovisc-into-the-anterior-chamber-improved-corneal-preservation-and-transplantation-for-cornea-blind-patients
#11
Bing Li, Li Chen, Yihui Chen, Jiajun Lu, Hao Liu, Yu Liu, Panpan Yao, Rui Liu, Minjie Sheng
PURPOSE: In this study, we aimed to provide a new method of corneal preservation by injecting DisCoVisc into the anterior chamber of eyeballs and evaluate its efficiency for corneal transplantation. METHODS: Three pairs of eyeballs (n=6) were preserved by DisCoVisc viscoelastic agent, and the corneas were stored for 1 to 6 days. Then, the structure and morphology of cornea were analyzed by hematoxylin-eosin (H&E) staining, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, and transmission electron microscopy (TEM)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28977017/real-time-assessment-of-corneal-endothelial-cell-damage-following-graft-preparation-and-donor-insertion-for-dmek
#12
Maninder Bhogal, Chan N Lwin, Xin-Yi Seah, Elavazhagan Murugan, Khadijah Adnan, Shu-Jun Lin, Gary Peh, Jodhbir S Mehta
PURPOSE: To establish a method for assessing graft viability, in-vivo, following corneal transplantation. METHODS: Optimization of calcein AM fluorescence and toxicity assessment was performed in cultured human corneal endothelial cells and ex-vivo corneal tissue. Descemet membrane endothelial keratoplasty grafts were incubated with calcein AM and imaged pre and post preparation, and in-situ after insertion and unfolding in a pig eye model. Global, macroscopic images of the entire graft and individual cell resolution could be attained by altering the magnification of a clinical confocal scanning laser microscope...
2017: PloS One
https://www.readbyqxmd.com/read/28976415/amniotic-membrane-grafts-to-reduce-pterygium-recurrence
#13
Ronald Rosen
PURPOSE: To demonstrate the long-term outcome of pterygium surgery with adjunctive amniotic membrane transplantation (AMT) and intraoperative mitomycin C (MMC) in a large case series. METHODS: Medical records were retrieved for this noncomparative retrospective study of all patients who had pterygium excision with adjunctive AMT and intraoperative MMC, from October 2010 to June 2016 with at least 6 months of follow-up. RESULTS: There were a total of 556 eyes of 535 patients (291 males and 244 females) with average age of 51...
October 2, 2017: Cornea
https://www.readbyqxmd.com/read/28974839/phacoemulsification-after-penetrating-keratoplasty-due-war-injury-at-young-adult
#14
Emir Cabric, Vahid Jusufovic, Nizama Salihefendic, Amra Nadarevic Vodencarevic
INTRODUCTION: It is known that phacoemulsification of cataracts after penetrating keratoplasty there are always some difficulties and of course a higher rate of different intraoperative complications. Phacoemulsification after PK may cause significant endothelial injury and affect long term graft survival. AIM: The aim of this report is to describe one of these cases and the possible ways to manage them. CASE REPORT: We report a case o of a 31-year-old female patient, with a cataract on her left eye...
June 2017: Medical Archives
https://www.readbyqxmd.com/read/28973097/association-between-fungal-contamination-and-eye-bank-prepared-endothelial-keratoplasty-tissue-temperature-dependent-risk-factors-and-antifungal-supplementation-of-optisol-gentamicin-and-streptomycin
#15
Kimberly M Brothers, Robert M Q Shanks, Susan Hurlbert, Regis P Kowalski, Elmer Y Tu
Importance: Fungal contamination and infection from donor tissues processed for endothelial keratoplasty is a growing concern, prompting analysis of donor tissues after processing. Objective: To determine whether eyebank-processed endothelial keratoplasty tissue is at higher risk of contamination than unprocessed tissue and to model eyebank processing with regard to room temperature exposure on Candida growth in optisol-gentamicin and streptomycin (GS) with and without antifungal supplementation...
September 28, 2017: JAMA Ophthalmology
https://www.readbyqxmd.com/read/28971253/-block-excision-with-tectonic-corneoscleral-graft-for-traumatic-pediatric-epithelial-implantation-cysts
#16
G Avgitidou, L M Heindl, C Cursiefen
Invasion of corneal epithelium into the anterior chamber can arise after ocular surgery or penetrating injury. This is extremly rare with an incidence of 0-0.2%. Three different types can be distiguished: cystic, diffuse and mixed form. Only cystic epithelial invasion can be treated with a curative therapeutic option that is blockexzision with tectonic corneoscleral transplantation.
September 29, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/28970517/human-in-vitro-model-reveals-the-effects-of-collagen-cross-linking-on-keratoconus-pathogenesis
#17
Rabab Sharif, Jesper Hjortdal, Henrik Sejersen, Garett Frank, Dimitrios Karamichos
Keratoconus (KC) is a corneal thinning disorder that leads to severe vision impairment As opposed to corneal transplantation; corneal collagen crosslinking (CXL) is a relatively non-invasive procedure that leads to an increase in corneal stiffness. In order to evaluate the effect of CXL on human corneal stromal cells in vitro, we developed a 3-D in vitro CXL model, using primary Human corneal fibroblasts (HCFs) from healthy patients and Human Keratoconus fibroblasts (HKCs) from KC patients. Cells were plated on transwell polycarbonate membranes and stimulated by a stable vitamin C...
October 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28969612/willingness-to-donate-eyes-and-its-associated-factors-among-adults-in-gondar-town-north-west-ethiopia
#18
Mohammed Seid Hussen, Kbrom Legesse Gebreselassie, Asamere Tsegaw Woredekal, Nebiyat Feleke Adimassu
BACKGROUND: In Ethiopia, there is a substantial mismatch between need and supply of corneal transplant. Although corneal transplantation service is affected by various factors, willingness to donate eyes is an essential indicator of its availability, accessibility, and acceptability. Therefore, this study aimed to determine the magnitude of willingness to donate eyes and its associated factors, which help to develop appropriate strategies that can address this undersupply and unmet need...
October 2, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28959505/update-on-the-management-of-high-risk-penetrating-keratoplasty
#19
Sayena Jabbehdari, Alireza Baradaran Rafii, Ghasem Yazdanpanah, Pedram Hamrah, Edward J Holland, Ali R Djalilian
PURPOSE OF REVIEW: In this article, we review the indications and latest management of high-risk penetrating keratoplasty. RECENT FINDINGS: Despite the immune-privilege status of the cornea, immune-mediated graft rejection still remains the leading cause of corneal graft failure. This is particularly a problem in the high-risk graft recipients, namely patients with previous graft failure due to rejection and those with inflamed and vascularized corneal beds. A number of strategies including both local and systemic immunosuppression are currently used to increase the success rate of high-risk corneal grafts...
March 2017: Current Ophthalmology Reports
https://www.readbyqxmd.com/read/28957444/optimizing-the-role-of-limbal-explant-size-and-source-in-determining-the-outcomes-of-limbal-transplantation-an-in-vitro-study
#20
Abhinav Reddy Kethiri, Sayan Basu, Sachin Shukla, Virender Singh Sangwan, Vivek Singh
PURPOSE: Simple limbal epithelial transplantation (SLET) and cultivated limbal epithelial transplantation (CLET) are proven clinical techniques for treating limbal stem cell deficiency (LSCD). However, the ideal size and number of the limbal explants required for transplantation has not been clearly elucidated. This in vitro study aimed to determine the optimal limbal explant size required for complete corneal epithelialization by characterizing the cell expansion. METHODS: Limbal explants obtained from both live and cadaveric biopsies were cultured on the denuded amniotic membrane...
2017: PloS One
keyword
keyword
51608
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"