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T cell facs analysis

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https://www.readbyqxmd.com/read/29906526/promidis%C3%AE-a-tcr%C3%AE-signature-associated-with-immunodeficiencies-caused-by-v-d-j-recombination-defects
#1
Aurélie Berland, Jérémie Rosain, Sophie Kaltenbach, Vincent Allain, Nizar Mahlaoui, Isabelle Melki, Alice Fievet, Catherine Dubois d'Enghien, Marie Ouachée-Chardin, Laurence Perrin, Nathalie Auger, Funda Erol Cipe, Andrea Finocchi, Figen Dogu, Felipe Suarez, Despina Moshous, Thierry Leblanc, Alexandre Belot, Claire Fieschi, David Boutboul, Marion Malphettes, Lionel Galicier, Eric Oksenhendler, Stéphane Blanche, Alain Fischer, Patrick Revy, Dominique Stoppa-Lyonnet, Capucine Picard, Jean-Pierre de Villartay
- BACKGROUND: V(D)J recombination ensures the diversity of the adaptive immune system. While its complete defect causes Severe Combined Immunodeficiency (T-B-SCID), its suboptimal activity, is associated with a broad spectrum of immune manifestations such as late onset combined immunodeficiency and autoimmunity. The earliest molecular diagnosis of these patients is required to adopt the best therapy strategy, in particular when it involves myelo-ablative conditioning regimen for hematopoietic stem cell transplantation (HSCT)...
June 12, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29859535/toll-like-receptor-2-activation-primes-and-upregulates-osteoclastogenesis-via-lox-1
#2
Kimiko Ohgi, Hiroshi Kajiya, Kazuko Goto-T, Fujio Okamoto, Yasunori Yoshinaga, Koji Okabe, Ryuji Sakagami
BACKGROUND: Lectin-like oxidized low-density-lipoprotein receptor 1 (Lox-1) is the receptor for oxidized low-density lipoprotein (oxLDL), a mediator in dyslipidemia. Toll-like receptor (TLR)-2 and - 4 are receptors of lipopolysaccharide (LPS) from Porphyromonas gingivalis, a major pathogen of chronic periodontitis. Although some reports have demonstrated that periodontitis has an adverse effect on dyslipidemia, little is clear that the mechanism is explained the effects of dyslipidemia on osteoclastogenesis...
June 2, 2018: Lipids in Health and Disease
https://www.readbyqxmd.com/read/29850627/gene-expression-profiling-in-behcet-s-disease-indicates-an-autoimmune-component-in-the-pathogenesis-of-the-disease-and-opens-new-avenues-for-targeted-therapy
#3
Antonio Puccetti, Piera Filomena Fiore, Andrea Pelosi, Elisa Tinazzi, Giuseppe Patuzzo, Giuseppe Argentino, Francesca Moretta, Claudio Lunardi, Marzia Dolcino
Behçet disease (BD) is a chronic inflammatory multisystem disease characterized by oral and genital ulcers, uveitis, and skin lesions. Disease etiopathogenesis is still unclear. We aim to elucidate some aspects of BD pathogenesis and to identify specific gene signatures in peripheral blood cells (PBCs) of patients with active disease using novel gene expression and network analysis. 179 genes were modulated in 10 PBCs of BD patients when compared to 10 healthy donors. Among differentially expressed genes the top enriched gene function was immune response, characterized by upregulation of Th17-related genes and type I interferon- (IFN-) inducible genes...
2018: Journal of Immunology Research
https://www.readbyqxmd.com/read/29799396/circulating-cd4-cd8-double-positive-t-cells-display-features-of-innate-and-adaptive-immune-function-in-granulomatosis-with-polyangiitis
#4
Anja Kerstein, Antje Müller, Silke Pitann, Gabriela Riemekasten, Peter Lamprecht
OBJECTIVES: To examine functional features of CD4+CD8+ double-positive T-cells in patients with granulomatosis with polyangiitis (GPA) using phenotypic and transcriptomic analysis. METHODS: Staining of cellular surface marker was performed using freshly collected whole blood. For intracellular cytokine staining freshly collected whole blood was stimulated with phorbol myristate acetate and ionomycin. Multicolor flow cytometric analysis was performed on a FACSCanto II cytometer using FACSDiva software...
March 2018: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/29760930/prevalence-of-thrombocytopenia-before-and-after-initiation-of-haart-among-hiv-infected-patients-at-black-lion-specialized-hospital-addis-ababa-ethiopia-a-cross-sectional-study
#5
Gashaw Garedew Woldeamanuel, Diresibachew Haile Wondimu
Background: Hematological abnormalities are common in HIV positive patients. Of these, thrombocytopenia is a known complication which has been associated with a variety of bleeding disorders. However, its magnitude and related factors have not been well-characterized in the era of highly active antiretroviral therapy (HAART) in Ethiopia. Therefore, this study aimed to assess the prevalence of thrombocytopenia before and after initiation of HAART among HIV positive patients attending Black Lion Specialized Hospital, Addis Ababa, Ethiopia...
2018: BMC Hematology
https://www.readbyqxmd.com/read/29720975/matrix-remodeling-associated-7-deficiency-alleviates-carbon-tetrachloride-induced-acute-liver-injury-in-mice
#6
Dandan Lin, Zhenjiang Sun, Ziqi Jin, Lei Lei, Yonghao Liu, Bo Hu, Benfang Wang, Ying Shen, Yiqiang Wang
Matrix remodeling associated 7 (MXRA7) was first noted to co-express with a group of matrix remodeling related genes, and its biological functions had remained unclear. In this study, we investigated the presumed function of MXRA7 in a carbon tetrachloride (CCl4 )-induced acute liver injury model in mice. Wild-type, MXRA7-/- mice, and mice that were pulsed with hydrodynamic injection of vehicle or MXRA7-harboring plasmids were challenged with a single dose of CCl4 for injury induction. The sera, spleens, and livers were harvested from mice for assay of cytokines/chemokines expression, cellular responses, or histological features...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29713426/extracellular-vesicles-from-bone-marrow-derived-mesenchymal-stromal-cells-support-ex-vivo-survival-of-human-antibody-secreting-cells
#7
Doan C Nguyen, Holly C Lewis, Chester Joyner, Vivien Warren, Haopeng Xiao, Haydn T Kissick, Ronghu Wu, Jacques Galipeau, F Eun-Hyung Lee
Extracellular vesicles (EVs) from bone marrow (BM)-derived mesenchymal stromal cells (BM-MSC) are novel mechanisms of cell-cell communication over short and long distances. BM-MSC have been shown to support human antibody secreting cells (ASC) survival ex vivo , but whether the crosstalk between the MSC-ASC interaction can occur via EVs is not known. Thus, we evaluated the role of EVs in ASC survival and IgG secretion. EVs were isolated from irradiated and non-irradiated primary BM-MSC and were quantified. They were further characterized by electron microscopy (EM) and CD63 and CD81 immuno-gold EM staining...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29618376/pseudoprogression-manifesting-as-recurrent-ascites-with-anti-pd-1-immunotherapy-in-urothelial-bladder-cancer
#8
Randy F Sweis, Yuanyuan Zha, Lomax Pass, Brian Heiss, Tara Chongsuwat, Jason J Luke, Thomas F Gajewski, Russell Szmulewitz
BACKGROUND: Immunotherapies targeting the PD-1 checkpoint pathway have recently gained regulatory approval in numerous cancer types. With the widespread use of immune checkpoint therapies, varying patterns of responses and immune-related adverse events are being observed. CASE PRESENTATION: In this case, we highlight a patient who developed recurrent, large-volume ascites, while simultaneously having a 49% reduction in peritoneal tumor lesion size by RECIST criteria...
April 4, 2018: Journal for Immunotherapy of Cancer
https://www.readbyqxmd.com/read/29593715/comparative-analysis-of-the-regulatory-t-cells-dynamics-in-peripheral-blood-in-human-and-porcine-polytrauma
#9
Rafael Serve, Ramona Sturm, Lukas Schimunek, Philipp Störmann, David Heftrig, Michel P J Teuben, Elsie Oppermann, Klemens Horst, Roman Pfeifer, Tim P Simon, Yannik Kalbas, Hans-Christoph Pape, Frank Hildebrand, Ingo Marzi, Borna Relja
Background: Severely injured patients experience substantial immunological stress in the aftermath of traumatic insult, which often results in systemic immune dysregulation. Regulatory T cells (Treg) play a key role in the suppression of the immune response and in the maintenance of immunological homeostasis. Little is known about their presence and dynamics in blood after trauma, and nothing is known about Treg in the porcine polytrauma model. Here, we assessed different subsets of Treg in trauma patients (TP) and compared those to either healthy volunteers (HV) or data from porcine polytrauma...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29573052/a-homozygous-variant-disrupting-the-pigh-start-codon-is-associated-with-developmental-delay-epilepsy-and-microcephaly
#10
Alistair T Pagnamenta, Yoshiko Murakami, Consuelo Anzilotti, Hannah Titheradge, Adam J Oates, Jenny Morton, Taroh Kinoshita, Usha Kini, Jenny C Taylor
Defective glycosylphosphatidylinositol (GPI)-anchor biogenesis can cause a spectrum of predominantly neurological problems. For eight genes critical to this biological process, disease associations are not yet reported. Scanning exomes from 7,833 parent-child trios and 1,792 singletons from the DDD study for biallelic variants in this gene-set uncovered a rare PIGH variant in a boy with epilepsy, microcephaly, and behavioral difficulties. Although only 2/2 reads harbored this c.1A > T transversion, the presence of ∼25 Mb autozygosity at this locus implied homozygosity, which was confirmed using Sanger sequencing...
March 23, 2018: Human Mutation
https://www.readbyqxmd.com/read/29568529/prevalence-of-anemia-before-and-after-initiation-of-antiretroviral-therapy-among-hiv-infected-patients-at-black-lion-specialized-hospital-addis-ababa-ethiopia-a-cross-sectional-study
#11
Gashaw Garedew Woldeamanuel, Diresibachew Haile Wondimu
Background: Anemia is the most common hematological abnormality in Human immunodeficiency virus (HIV) positive patients and a significant predictor of its progression to AIDS or death. This study was aimed to assess the prevalence of anemia before and after initiation of antiretroviral therapy (ART) among HIV positive patients attending Black Lion Specialized Hospital, Addis Ababa, Ethiopia. Methods: A cross sectional study was conducted from January to April, 2017 in Black Lion Specialized Hospital, Addis Ababa, Ethiopia...
2018: BMC Hematology
https://www.readbyqxmd.com/read/29567371/a-pilot-study-on-immuno-psychiatry-in-the-22q11-2-deletion-syndrome-a-role-for-th17-cells-in-psychosis
#12
Elfi Vergaelen, Carmen Schiweck, Kristof Van Steeland, Jacqueline Counotte, Wim Veling, Ann Swillen, Hemmo Drexhage, Stephan Claes
BACKGROUND: A growing body of evidence supports a role for immune alterations in Schizophrenia Spectrum Disorders (SSD). A high prevalence (25-40%) of SSD has been found in patients with 22q11.2 deletion syndrome (22q11.2DS), which is known for T-cell deficits due to thymus hypoplasia. This study is the first to explore the association between the T-cell subsets and psychotic symptoms in adults with 22q11.2DS. METHODS: 34 individuals (aged 19-38 yrs.) with 22q11...
May 2018: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29560431/cd4-t-cells-react-to-local-increase-of-%C3%AE-synuclein-in-a-pathology-associated-variant-dependent-manner-and-modify-brain-microglia-in-absence-of-brain-pathology
#13
Mads N Olesen, Josefine R Christiansen, Steen Vang Petersen, Poul Henning Jensen, Wojciech Paslawski, Marina Romero-Ramos, Vanesa Sanchez-Guajardo
We have previously shown that immunological processes in the brain during α-synuclein-induced neurodegeneration vary depending on the presence or absence of cell death. This suggests that the immune system is able to react differently to the different stages of α-synuclein pathology. However, it was unclear whether these immune changes were governed by brain processes or by a direct immune response to α-synuclein modifications. We have herein locally increased the peripheral concentration of α-synuclein or its pathology-associated variants, nitrated or fibrillar, to characterize the modulation of the CD4 T cell pool by α-synuclein and brain microglia in the absence of any α-synuclein brain pathology...
January 2018: Heliyon
https://www.readbyqxmd.com/read/29545366/atlas-of-the-immune-cell-repertoire-in-mouse-atherosclerosis-defined-by-single-cell-rna-sequencing-and-mass-cytometry
#14
Holger Winkels, Erik Ehinger, Melanie Vassallo, Konrad Buscher, Huy Q Dinh, Kouji Kobiyama, Anouk A J Hamers, Clément Cochain, Ehsan Vafadarnejad, Antoine-Emmanuel Saliba, Alma Zernecke, Akula Bala Pramod, Amlan K Ghosh, Nathaly Anto Michel, Natalie Hoppe, Ingo Hilgendorf, Andreas Zirlik, Catherine C Hedrick, Klaus Ley, Dennis Wolf
RATIONALE: Atherosclerosis is a chronic inflammatory disease that is driven by the interplay of pro- and anti-inflammatory leukocytes in the aorta. Yet, the phenotypic and transcriptional diversity of aortic leukocytes is poorly understood. OBJECTIVE: We characterized leukocytes from healthy and atherosclerotic mouse aortas in-depth by single-cell RNA-sequencing and mass cytometry (cytometry by time of flight) to define an atlas of the immune cell landscape in atherosclerosis...
June 8, 2018: Circulation Research
https://www.readbyqxmd.com/read/29524520/the-tumor-antagonistic-steroidal-alkaloid-solanidine-prompts-the-intrinsic-suicidal-signal-mediated-dff-40-nuclear-import-and-nucleosomal-disruption
#15
Vikas H Malojirao, V Vigneshwaran, Prabhu Thirusangu, Riaz Mahmood, B T Prabhakar
Aim Deformity in the cellular homeostatic event associated with cell survival and apoptosis are committing factors for carcinogenesis. Interventions of these events by pharmacologically active agent gain predominance in cancer treatment. In current investigation Solanidine, a steroidal alkaloid was evaluated on tumorigenesis by targeting death signal using multiple tumor cells and model systems. MAIN METHODS: Anti-proliferative effect was evaluated using cytotoxic studies. Prolonged cytotoxic effect of Solanidine was examined by colony formation assay...
April 15, 2018: Life Sciences
https://www.readbyqxmd.com/read/29507859/-in-vitro-intracellular-ifn%C3%AE-il-17-and-il-10-producing-t-cells-correlates-with-the-occurrence-of-post-transplant-opportunistic-infection-in-liver-and-kidney-recipients
#16
Francisco Boix, Santiago Llorente, Jorge Eguía, Gema Gonzalez-Martinez, Rafael Alfaro, Jose A Galián, Jose A Campillo, María Rosa Moya-Quiles, Alfredo Minguela, Jose A Pons, Manuel Muro
AIM: To validate intracellular cytokine production functional assay as means of cell-mediated immunity monitoring of post-transplant patients with opportunistic infection (OI). METHODS: Intracellular cytokine-producing CD4+ and CD8+ T-cell monitoring was carried out in 30 liver transplant (LTr) and 31 kidney transplant (KTr) recipients from 2010 to 2012. Patients were assessed in our Department of Immunology at the Clinical University 'Hospital Virgen de la Arrixaca-IMIB' in Murcia, Spain for one year following transplantation...
February 24, 2018: World Journal of Transplantation
https://www.readbyqxmd.com/read/29491310/a-case-of-fulminant-type-1-diabetes-mellitus-accompanied-by-positive-conversion-of-anti-insulin-antibody-after-the-administration-of-anti-ctla-4-antibody-following-the-discontinuation-of-anti-pd-1-antibody
#17
Michiru Shiba, Hidefumi Inaba, Hiroyuki Ariyasu, Shintaro Kawai, Yuko Inagaki, Shohei Matsuno, Hiroshi Iwakura, Yuki Yamamoto, Masahiro Nishi, Takashi Akamizu
An 80-year-old woman with malignant melanoma received 20 cycles of anti-PD-1 antibody (nivolumab) treatment and showed normal glucose tolerance. Three weeks after switching to anti-CTLA-4 antibody (ipilimumab), her plasma glucose level was elevated to 639 mg/dL, her HbA1c was 7.7%, and her fastening serum CPR was undetectable. Anti-GAD and IA-2 antibodies were negative. She was diagnosed with fulminant type 1 diabetes mellitus (F1DM). Remarkably, her anti-insulin antibody was positively converted, and her KL-6 levels increased after ipilimumab therapy...
February 28, 2018: Internal Medicine
https://www.readbyqxmd.com/read/29458078/isolation-of-intact-rna-from-murine-cd4-t-cells-after-intracellular-cytokine-staining-and-fluorescence-activated-cell-sorting
#18
Shajo Kunnath-Velayudhan, Steven A Porcelli
Intracellular cytokine staining (ICS) is a powerful method for identifying functionally distinct lymphocyte subsets, and for isolating these by fluorescence activated cell sorting (FACS). Although transcriptomic analysis of cells sorted on the basis of ICS has many potential applications, this is rarely performed because of the difficulty in isolating intact RNA from cells processed using standard fixation and permeabilization buffers for ICS. To address this issue, we compared three buffers shown previously to preserve RNA in nonhematopoietic cells subjected to intracellular staining for their effects on RNA isolated from T lymphocytes processed for ICS...
May 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29448043/estrogen-serum-concentration-affects-blood-immune-cell-composition-and-polarization-in-human-females-under-controlled-ovarian-stimulation
#19
Pardes Habib, Daniela Dreymueller, Benjamin Rösing, Hannes Botung, Alexander Slowik, Adib Zendedel, Shahin Habib, Stefanie Hoffmann, Cordian Beyer
Estrogens modulate the immune system and possess anti-inflammatory properties. In line, immune cells express a variety of estrogen receptors (ER) including ER-alpha and -beta. In the present study, we examined the influence of 17beta-estradiol (E2) serum concentrations on blood leukocyte composition and their ex vivo polarization/activation status by FACS analysis in sub-fertile human females under controlled ovarian stimulation (COS). Using a set of cell-type and polarization-specific markers, we demonstrate that increased 17ß-estradiol (E2) serum concentrations yield an overall increase in leukocytes, neutrophils and monocytes but decreased lymphocytes...
April 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29432774/proline-serine-threonine-phosphatase-interacting-protein-1-pstpip1-controls-immune-synapse-stability-in-human-t-cells
#20
Willemijn J M Janssen, Valeria Grobarova, Jardin Leleux, Lieneke Jongeneel, Marielle van Gijn, Joris M van Montfrans, Marianne Boes
BACKGROUND: Proline-serine-threonine phosphatase interacting protein 1 (PSTPIP1) is a cytosolic adaptor protein involved with T-cell activation, differentiation, and migration. On cognate T-cell contact, PSTPIP1 is recruited to surface-expressed CD2, where it regulates F-actin remodeling. An immune synapse (IS) is thereby rapidly formed, consisting of T-cell receptor clusters surrounded by a ring of adhesion molecules, including CD2. OBJECTIVE: From genetic screening of patients with primary immunodeficiencies, we identified 2 mutations in PSTPIP1, R228C and T274M, which we further characterized in the primary patients' T cells...
February 9, 2018: Journal of Allergy and Clinical Immunology
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