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T cell facs analysis

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https://www.readbyqxmd.com/read/29618376/pseudoprogression-manifesting-as-recurrent-ascites-with-anti-pd-1-immunotherapy-in-urothelial-bladder-cancer
#1
Randy F Sweis, Yuanyuan Zha, Lomax Pass, Brian Heiss, Tara Chongsuwat, Jason J Luke, Thomas F Gajewski, Russell Szmulewitz
BACKGROUND: Immunotherapies targeting the PD-1 checkpoint pathway have recently gained regulatory approval in numerous cancer types. With the widespread use of immune checkpoint therapies, varying patterns of responses and immune-related adverse events are being observed. CASE PRESENTATION: In this case, we highlight a patient who developed recurrent, large-volume ascites, while simultaneously having a 49% reduction in peritoneal tumor lesion size by RECIST criteria...
April 4, 2018: Journal for Immunotherapy of Cancer
https://www.readbyqxmd.com/read/29593715/comparative-analysis-of-the-regulatory-t-cells-dynamics-in-peripheral-blood-in-human-and-porcine-polytrauma
#2
Rafael Serve, Ramona Sturm, Lukas Schimunek, Philipp Störmann, David Heftrig, Michel P J Teuben, Elsie Oppermann, Klemens Horst, Roman Pfeifer, Tim P Simon, Yannik Kalbas, Hans-Christoph Pape, Frank Hildebrand, Ingo Marzi, Borna Relja
Background: Severely injured patients experience substantial immunological stress in the aftermath of traumatic insult, which often results in systemic immune dysregulation. Regulatory T cells (Treg) play a key role in the suppression of the immune response and in the maintenance of immunological homeostasis. Little is known about their presence and dynamics in blood after trauma, and nothing is known about Treg in the porcine polytrauma model. Here, we assessed different subsets of Treg in trauma patients (TP) and compared those to either healthy volunteers (HV) or data from porcine polytrauma...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29573052/a-homozygous-variant-disrupting-the-pigh-start-codon-is-associated-with-developmental-delay-epilepsy-and-microcephaly
#3
Alistair T Pagnamenta, Yoshiko Murakami, Consuelo Anzilotti, Hannah Titheradge, Adam J Oates, Jenny Morton, Taroh Kinoshita, Usha Kini, Jenny C Taylor
Defective GPI-anchor biogenesis can cause a spectrum of predominantly neurological problems. For 8 genes critical to this biological process, disease associations are not yet reported. Scanning exomes from 7833 parent-child trios and 1792 singletons from the DDD study for biallelic variants in this gene-set uncovered a rare PIGH variant in a boy with epilepsy, microcephaly and behavioural difficulties. Although only 2/2 reads harboured this c.1A > T transversion, the presence of ∼25Mb autozygosity at this locus implied homozygosity, which was confirmed using Sanger sequencing...
March 23, 2018: Human Mutation
https://www.readbyqxmd.com/read/29568529/prevalence-of-anemia-before-and-after-initiation-of-antiretroviral-therapy-among-hiv-infected-patients-at-black-lion-specialized-hospital-addis-ababa-ethiopia-a-cross-sectional-study
#4
Gashaw Garedew Woldeamanuel, Diresibachew Haile Wondimu
Background: Anemia is the most common hematological abnormality in Human immunodeficiency virus (HIV) positive patients and a significant predictor of its progression to AIDS or death. This study was aimed to assess the prevalence of anemia before and after initiation of antiretroviral therapy (ART) among HIV positive patients attending Black Lion Specialized Hospital, Addis Ababa, Ethiopia. Methods: A cross sectional study was conducted from January to April, 2017 in Black Lion Specialized Hospital, Addis Ababa, Ethiopia...
2018: BMC Hematology
https://www.readbyqxmd.com/read/29567371/a-pilot-study-on-immuno-psychiatry-in-the-22q11-2-deletion-syndrome-a-role-for-th17-cells-in-psychosis
#5
Elfi Vergaelen, Carmen Schiweck, Kristof Van Steeland, Jacqueline Counotte, Wim Veling, Ann Swillen, Hemmo Drexhage, Stephan Claes
BACKGROUND: A growing body of evidence supports a role for immune alterations in Schizophrenia Spectrum Disorders (SSD). A high prevalence (25% - 40%) of SSD has been found in patients with 22q11.2 deletion syndrome (22q11.2DS), which is known for T-cell deficits due to thymus hypoplasia. This study is the first to explore the association between the T-cell subsets and psychotic symptoms in adults with 22q11.2DS. METHODS: 34 individuals (aged 19-38 yrs.) with 22q11...
March 19, 2018: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29560431/cd4-t-cells-react-to-local-increase-of-%C3%AE-synuclein-in-a-pathology-associated-variant-dependent-manner-and-modify-brain-microglia-in-absence-of-brain-pathology
#6
Mads N Olesen, Josefine R Christiansen, Steen Vang Petersen, Poul Henning Jensen, Wojciech Paslawski, Marina Romero-Ramos, Vanesa Sanchez-Guajardo
We have previously shown that immunological processes in the brain during α-synuclein-induced neurodegeneration vary depending on the presence or absence of cell death. This suggests that the immune system is able to react differently to the different stages of α-synuclein pathology. However, it was unclear whether these immune changes were governed by brain processes or by a direct immune response to α-synuclein modifications. We have herein locally increased the peripheral concentration of α-synuclein or its pathology-associated variants, nitrated or fibrillar, to characterize the modulation of the CD4 T cell pool by α-synuclein and brain microglia in the absence of any α-synuclein brain pathology...
January 2018: Heliyon
https://www.readbyqxmd.com/read/29545366/atlas-of-the-immune-cell-repertoire-in-mouse-atherosclerosis-defined-by-single-cell-rna-sequencing-and-mass-cytometry
#7
Holger Winkels, Erik Ehinger, Melanie Vassallo, Konrad Buscher, Huy Dinh, Kouji Kobiyama, Anouk Hamers, Clément Cochain, Ehsan Vafadarnejad, Antoine-Emmanuel Saliba, Alma Zernecke, Akula Pramod, Amlan Ghosh, Nathaly Anto Michel, Natalie Hoppe, Ingo Hilgendorf, Andreas Zirlik, Catherine Hedrick, Klaus Ley, Dennis Wolf
<u>Rationale:</u> Atherosclerosis is a chronic inflammatory disease that is driven by the interplay of pro- and anti-inflammatory leukocytes in the aorta. Yet, the phenotypic and transcriptional diversity of aortic leukocytes is only poorly understood. <u>Objective:</u> We characterized leukocytes from healthy and atherosclerotic mouse aortas in-depth by single cell RNA-sequencing (scRNAseq) and mass cytometry (CyTOF) to define an atlas of the immune cell landscape in atherosclerosis...
March 15, 2018: Circulation Research
https://www.readbyqxmd.com/read/29524520/the-tumor-antagonistic-steroidal-alkaloid-solanidine-prompts-the-intrinsic-suicidal-signal-mediated-dff-40-nuclear-import-and-nucleosomal-disruption
#8
Vikas H Malojirao, V Vigneshwaran, Prabhu Thirusangu, Riaz Mahmood, B T Prabhakar
Aim Deformity in the cellular homeostatic event associated with cell survival and apoptosis are committing factors for carcinogenesis. Interventions of these events by pharmacologically active agent gain predominance in cancer treatment. In current investigation Solanidine, a steroidal alkaloid was evaluated on tumorigenesis by targeting death signal using multiple tumor cells and model systems. MAIN METHODS: Anti-proliferative effect was evaluated using cytotoxic studies. Prolonged cytotoxic effect of Solanidine was examined by colony formation assay...
March 7, 2018: Life Sciences
https://www.readbyqxmd.com/read/29507859/-in-vitro-intracellular-ifn%C3%AE-il-17-and-il-10-producing-t-cells-correlates-with-the-occurrence-of-post-transplant-opportunistic-infection-in-liver-and-kidney-recipients
#9
Francisco Boix, Santiago Llorente, Jorge Eguía, Gema Gonzalez-Martinez, Rafael Alfaro, Jose A Galián, Jose A Campillo, María Rosa Moya-Quiles, Alfredo Minguela, Jose A Pons, Manuel Muro
AIM: To validate intracellular cytokine production functional assay as means of cell-mediated immunity monitoring of post-transplant patients with opportunistic infection (OI). METHODS: Intracellular cytokine-producing CD4+ and CD8+ T-cell monitoring was carried out in 30 liver transplant (LTr) and 31 kidney transplant (KTr) recipients from 2010 to 2012. Patients were assessed in our Department of Immunology at the Clinical University 'Hospital Virgen de la Arrixaca-IMIB' in Murcia, Spain for one year following transplantation...
February 24, 2018: World Journal of Transplantation
https://www.readbyqxmd.com/read/29491310/a-case-of-fulminant-type-1-diabetes-mellitus-accompanied-by-positive-conversion-of-anti-insulin-antibody-after-the-administration-of-anti-ctla-4-antibody-following-the-discontinuation-of-anti-pd-1-antibody
#10
Michiru Shiba, Hidefumi Inaba, Hiroyuki Ariyasu, Shintaro Kawai, Yuko Inagaki, Shohei Matsuno, Hiroshi Iwakura, Yuki Yamamoto, Masahiro Nishi, Takashi Akamizu
An 80-year-old woman with malignant melanoma received 20 cycles of anti-PD-1 antibody (nivolumab) treatment and showed normal glucose tolerance. Three weeks after switching to anti-CTLA-4 antibody (ipilimumab), her plasma glucose level was elevated to 639 mg/dL, her HbA1c was 7.7%, and her fastening serum CPR was undetectable. Anti-GAD and IA-2 antibodies were negative. She was diagnosed with fulminant type 1 diabetes mellitus (F1DM). Remarkably, her anti-insulin antibody was positively converted, and her KL-6 levels increased after ipilimumab therapy...
February 28, 2018: Internal Medicine
https://www.readbyqxmd.com/read/29458078/isolation-of-intact-rna-from-murine-cd4-t-cells-after-intracellular-cytokine-staining-and-fluorescence-activated-cell-sorting
#11
Shajo Kunnath-Velayudhan, Steven A Porcelli
Intracellular cytokine staining (ICS) is a powerful method for identifying functionally distinct lymphocyte subsets, and for isolating these by fluorescence activated cell sorting (FACS). Although transcriptomic analysis of cells sorted on the basis of ICS has many potential applications, this is rarely performed because of the difficulty in isolating intact RNA from cells processed using standard fixation and permeabilization buffers for ICS. To address this issue, we compared three buffers shown previously to preserve RNA in nonhematopoietic cells subjected to intracellular staining for their effects on RNA isolated from T lymphocytes processed for ICS...
February 17, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29448043/estrogen-serum-concentration-affects-blood-immune-cell-composition-and-polarization-in-human-females-under-controlled-ovarian-stimulation
#12
Pardes Habib, Daniela Dreymueller, Benjamin Rösing, Hannes Botung, Alexander Slowik, Adib Zendedel, Shahin Habib, Stefanie Hoffmann, Cordian Beyer
Estrogens modulate the immune system and possess anti-inflammatory properties. In line, immune cells express a variety of estrogen receptors (ER) including ER-alpha and -beta. In the present study, we examined the influence of 17beta-estradiol (E2) serum concentrations on blood leukocyte composition and their ex vivo polarization/activation status by FACS analysis in sub-fertile human females under controlled ovarian stimulation (COS). Using a set of cell-type and polarization-specific markers, we demonstrate that increased 17ß-estradiol (E2) serum concentrations yield an overall increase in leukocytes, neutrophils and monocytes but decreased lymphocytes...
February 12, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29432774/pstpip1-controls-immune-synapse-stability-in-human-t-cells
#13
W J M Janssen, V Grobarova, J Leleux, C H Jongeneel, M van Gijn, J M van Montfrans, M Boes
BACKGROUND: PSTPIP1 is a cytosolic adaptor protein involved with T-cell activation, differentiation, and migration. Upon cognate T-cell contact, PSTPIP1 is recruited to surface-expressed CD2, where it regulates f-actin remodeling. An immune synapse (IS) is thereby rapidly formed, consisting of TCR clusters surrounded by a ring of adhesion molecules including CD2. OBJECTIVE: From genetic screening of primary immunodeficiency patients, we identified two mutations in PSTPIP1, R228C and T274M which we further characterized in primary patient T-cells...
February 9, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29396668/targeting-ataxia-telangiectasia-mutated-and-rad3-related-kinase-atr-in-pten-deficient-breast-cancers-for-personalized-therapy
#14
Nouf Al-Subhi, Reem Ali, Tarek Abdel-Fatah, Paul M Moseley, Stephen Y T Chan, Andrew R Green, Ian O Ellis, Emad A Rakha, Srinivasan Madhusudan
PURPOSE: Phosphate and tensin homolog (PTEN), a negative regulator of PI3K signaling, is involved in DNA repair. ATR is a key sensor of DNA damage and replication stress. We evaluated whether ATR signaling has clinical significance and could be targeted by synthetic lethality in PTEN-deficient triple-negative breast cancer (TNBC). METHODS: PTEN, ATR and pCHK1Ser345 protein level was evaluated in 1650 human breast cancers. ATR blockade by VE-821 was investigated in PTEN-proficient- (MDA-MB-231) and PTEN-deficient (BT-549, MDA-MB-468) TNBC cell lines...
February 2, 2018: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/29371108/the-importance-of-area-scaling-with-facs-diva-software
#15
Amy L Hazen, Timothy Bushnell, David L Haviland
With the release and use of the Becton Dickenson FACS Diva Software, the use of Area as the default parameter came into play. As such, the use of area as a calculated parameter, methods were needed to be employed to ensure doublet discrimination and proper display on standard FSC/SSC. Improper setting of forward area scaling can alter the display cell populations. This combined with improper area gating strategy can lead to doublet inclusion which in sorting rare events can compromise sort purity. In extreme cases where area scaling with the individual lasers is ignored, differences can exist between Area and Height where compensation will likely not be optimal, particularly if one parameter - usually height is saturated...
February 1, 2018: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29314593/in-vivo-immuno-reactivity-analysis-of-the-porous-three-dimensional-chitosan-sio-2-and-chitosan-sio-2-hydroxyapatite-hybrids
#16
Mengxia Guo, Yifan Dong, Jiangwei Xiao, Ruicai Gu, Maochao Ding, Tao Huang, Junhua Li, Naru Zhao, Hua Liao
Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology. However, the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions is still poorly defined. In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO2 ) and chitosan-silica-hydroxyapatite (CS/SiO2 /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately...
May 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29311117/expanded-genomic-profiling-of-circulating-tumor-cells-in-metastatic-breast-cancer-patients-to-assess-biomarker-status-and-biology-over-time-calgb-40502-and-calgb-40503-alliance
#17
Mark Jesus M Magbanua, Hope S Rugo, Denise M Wolf, Louai Hauranieh, Ritu Roy, Praveen Pendyala, Eduardo V Sosa, Janet H Scott, Jin Sun Lee, Brandelyn Pitcher, Terry Hyslop, William T Barry, Steven J Isakoff, Maura Dickler, Laura Van't Veer, John W Park
Purpose: We profiled circulating tumor cells (CTCs) to study the biology of blood-borne metastasis and to monitor biomarker status in metastatic breast cancer (MBC). Methods: CTCs were isolated from 105 patients with MBC using EPCAM-based immunomagnetic enrichment and fluorescence-activated cells sorting (IE/FACS), 28 of whom had serial CTC analysis (74 samples, 2-5 time points). CTCs were subjected to microfluidic-based multiplex QPCR array of 64 cancer-related genes ( n = 151) and genome-wide copy-number analysis by array comparative genomic hybridization (aCGH; n = 49)...
March 15, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29282022/chromosomal-instability-induced-by-increased-birc5-survivin-levels-affects-tumorigenicity-of-glioma-cells
#18
Marina Conde, Susanne Michen, Ralf Wiedemuth, Barbara Klink, Evelin Schröck, Gabriele Schackert, Achim Temme
BACKGROUND: Survivin, belonging to the inhibitor of apoptosis (IAP) gene family, is abundantly expressed in tumors. It has been hypothesized that Survivin facilitates carcinogenesis by inhibition of apoptosis resulting in improved survival of tumorigenic progeny. Additionally, Survivin plays an essential role during mitosis. Together with its molecular partners Aurora B, Borealin and inner centromere protein it secures bipolar chromosome segregation. However, whether increased Survivin levels contribute to progression of tumors by inducing chromosomal instability remains unclear...
December 28, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29250828/cd4-cd25-hi-foxp3-regulatory-t-cells-in-long-term-cardiac-xenotransplantation
#19
Avneesh K Singh, Joshua L Chan, Caleb N Seavey, Philip C Corcoran, Robert F Hoyt, Billeta G T Lewis, Marvin L Thomas, David L Ayares, Keith A Horvath, Muhammad M Mohiuddin
BACKGROUND: CD4+CD25Hi FoxP3+ T (Treg) cells are a small subset of CD4+ T cells that have been shown to exhibit immunoregulatory function. Although the absolute number of Treg cells in peripheral blood lymphocytes (PBL) is very small, they play an important role in suppressing immune reactivity. Several studies have demonstrated that the number of Treg cells, rather than their intrinsic suppressive capacity, may contribute to determining the long-term fate of transplanted grafts. In this study, we analyzed Treg cells in PBL of long-term baboon recipients who have received genetically modified cardiac xenografts from pig donors...
December 17, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29203363/mechanism-of-action-of-g-quadruplex-forming-oligonucleotide-homologous-to-the-telomere-overhang-in-melanoma
#20
Gagan Chhabra, Luke Wojdyla, Mark Frakes, Zachary Schrank, Brandon Leviskas, Marko Ivancich, Pooja Vinay, Ramesh Ganapathy, Benjamin E Ramirez, Neelu Puri
T-oligo, a guanine-rich oligonucleotide homologous to the 3'-telomeric overhang of telomeres, elicits potent DNA-damage responses in melanoma cells; however, its mechanism of action is largely unknown. Guanine-rich oligonucleotides can form G-quadruplexes (G4), which are stabilized by the hydrogen bonding of guanine residues. In this study, we confirmed the G4-forming capabilities of T-oligo using nondenaturing PAGE, nuclear magnetic resonance, and immunofluorescence. Using an anti-G-quadruplex antibody, we showed that T-oligo can form G4 in the nuclei of melanoma cells...
December 2, 2017: Journal of Investigative Dermatology
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