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metabolic response to surgical stress

I Schulze, E M Poos, H Meyer, A K List, S B R Kaestner, J Rehage
The aim of this study was to examine the effects of preoperative carprofen on the cardiorespiratory, hormonal and metabolic stress response during umbilical surgery under isoflurane anaesthesia combined with local anaesthesia, in calves. A randomised, blinded experimental study was conducted in 24 calves. Carprofen (n = 12; 1.4 mg/kg) or physiological saline solution (controls; n = 12) was administered 1 h prior to surgery. Anaesthesia was induced with xylazine (0.1 mg/kg, IM) and, after the onset of sedation (i...
October 2016: Veterinary Journal
Clair Hartmann, Sebastian Hafner, Angelika Scheuerle, Peter Möller, Markus Huber-Lang, Birgit Jung, Benedikt Nubaum, Oscar McCook, Michael Gröger, Florian Wagner, Sandra Weber, Bettina Stahl, Enrico Calzia, Michael Georgieff, Csaba Szabó, Rui Wang, Peter Radermacher, Katja Wagner
Pre-traumatic cigarette smoke (CS) exposure aggravates post-traumatic acute lung injury (ALI). Cystathionine-γ-lyase (CSE) protects against ALI and CS exposure-induced chronic obstructive lung disease (COPD). Therefore, we tested the hypothesis whether genetic CSE knockout (CSE) would aggravate post-traumatic ALI after CS exposure. After 3-4 weeks of CS exposure, anesthetized wild type (WT) and CSE mice underwent blunt chest trauma, surgical instrumentation and 4 hours of lung-protective mechanical ventilation...
September 28, 2016: Shock
Derek M Huffman, Jamie N Justice, Michael B Stout, James L Kirkland, Nir Barzilai, Steven N Austad
Life extension is no longer considered sufficient evidence of delayed aging in research animals. It must also be demonstrated that a broad swathe of health indicators have been extended. During a retreat of the Geroscience Network, a consortium of basic and clinical aging researchers, potential measures of mouse health were considered for their potential as easily standardized, highly informative metrics. Major health domains considered were neuromuscular, cognitive, cardiovascular, metabolic, and inflammatory functions as well as body composition and energetics and a multitude of assays interrogating these domains...
August 16, 2016: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
Yan Li, Qing Yuan, Jie Huang, Yi Ping Li, Jian Pan, Xing Feng, Xue Guang Zhang, Jiang Huai Wang, Jian Wang
BACKGROUND: Surgical stress initiates a series of host hormone, metabolism and immune responses, which predominantly affect the homeostatic mechanism of patients with major surgery. B7-H3 is a co-stimulatory molecule and has been shown to participate in both adaptive and innate immune responses. In this study we evaluated the clinical significance of plasma B7-H3 levels in pediatric patients with different types of operation and degrees of surgical stress. METHODS: A total of 48 children received pediatric general and cardiac surgery were recruited into this study...
2016: BMC Pediatrics
Louise E See Hoe, Lauren T May, John P Headrick, Jason N Peart
Disruption of the sarcolemmal membrane is a defining feature of oncotic death in cardiac ischaemia-reperfusion (I-R), and its molecular makeup not only fundamentally governs this process but also affects multiple determinants of both myocardial I-R injury and responsiveness to cardioprotective stimuli. Beyond the influences of membrane lipids on the cytoprotective (and death) receptors intimately embedded within this bilayer, myocardial ionic homeostasis, substrate metabolism, intercellular communication and electrical conduction are all sensitive to sarcolemmal makeup, and critical to outcomes from I-R...
October 2016: British Journal of Pharmacology
S Janßen, C Wunderlich, M Heppelmann, R Palme, A Starke, W Kehler, A Steiner, A Rizk, U Meyer, S Daenicke, J Rehage
Short-term effects of therapeutic claw trimming in acutely lame cows (n=21) with nonadvanced claw horn lesions on the endocrine, metabolic, and behavioral stress responses were investigated in comparison to regular claw trimming in nonlame control cows (n=21). Controls were matched to lame cows by parity and stage of lactation. Lame cows suffering from typical sole ulcers or white line disease were blinded and randomly assigned to 2 treatments, receiving 15 min before interventions either ketoprofen (n=11; 3mg/kg of BW intramuscularly; Romefen, Merial, Lyon, France) or placebo (n=10; saline in equivalent amount and route of administration)...
September 2016: Journal of Dairy Science
Aline Houessinon, Catherine François, Chloé Sauzay, Christophe Louandre, Gaelle Mongelard, Corinne Godin, Sandra Bodeau, Shinichiro Takahashi, Zuzana Saidak, Laurent Gutierrez, Jean-Marc Régimbeau, Nathalie Barget, Jean-Claude Barbare, Nathalie Ganne, Bruno Chauffert, Romain Coriat, Antoine Galmiche
BACKGROUND: Sorafenib, a kinase inhibitor active against various solid tumours, induces oxidative stress and ferroptosis, a new form of oxidative necrosis, in some cancer cells. Clinically-applicable biomarkers that reflect the impact of sorafenib on the redox metabolism of cancer cells are lacking. METHODS: We used gene expression microarrays, real-time PCR, immunoblot, protein-specific ELISA, and gene reporter constructs encoding the enzyme luciferase to study the response of a panel of cancer cells to sorafenib...
2016: Molecular Cancer
Malena B Rone, Qiao-Ling Cui, Jun Fang, Li-Chun Wang, Ji Zhang, Damla Khan, Melissa Bedard, Guillermina Almazan, Samuel K Ludwin, Russel Jones, Timothy E Kennedy, Jack P Antel
UNLABELLED: Multiple sclerosis (MS) lesions feature demyelination with limited remyelination. A distinct injury phenotype of MS lesions features dying back of oligodendrocyte (OL) terminal processes, a response that destabilizes myelin/axon interactions. This oligodendrogliopathy has been linked with local metabolic stress, similar to the penumbra of ischemic/hypoxic states. Here, we developed an in vitro oligodendrogliopathy model using human CNS-derived OLs and related this injury response to their distinct bioenergetic properties...
April 27, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Martin Matejovic, Zdenek Tuma, Jiri Moravec, Lenka Valesova, Roman Sykora, Jiri Chvojka, Jan Benes, Jan Mares
Although the burden of septic acute kidney injury continues to increase, the molecular pathogenesis remains largely obscure. The aim of this exploratory study was a discovery-driven analysis of dynamic kidney tissue protein expression changes applied for the first time in a classic large mammal model of sepsis. To achieve this goal, analyses of protein expression alterations were performed in serial samples of kidney cortical biopsies (before, 12 and 22 h of sepsis) in mechanically ventilated pigs challenged with continuous infusion of pseudomonas aeruginosa and compared with sham-operated control data...
October 2016: Shock
Guohao Wu
Perioperative patients usually suffer from metabolic response. This metabolic state is usually the result of some blend of response to partial starvation and to injury or specific diseases. Metabolic response to starvation and acute injury is inbuilt response to ensure maximal survival with a limited food intake. Yet, progressive loss of body tissue may have lethal consequences. Enhanced recovery after surgery is an optimized strategy of perioperative treatment based on variations of meta bolic state. It can reduce damage and acute injury, as well as facilitating early recovery after major surgery...
March 2016: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
Chaofu Ke, Ang Li, Yan Hou, Meng Sun, Kai Yang, Jinlong Cheng, Jingtao Wang, Tingting Ge, Fan Zhang, Qiang Li, Junnan Li, Ying Wu, Ge Lou, Kang Li
Epithelial ovarian cancer (EOC) is the most deadly of the gynecological cancers. New approaches and better tools for monitoring treatment efficacy and disease progression of EOC are required. In this study, metabolomics using rapid resolution liquid chromatography mass spectrometry was applied to a systematic investigation of metabolic changes in response to advanced EOC, surgery and recurrence. The results revealed considerable metabolic differences between groups. Moreover, 37, 30, and 26 metabolites were identified as potential biomarkers for primary, surgical and recurrent EOC, respectively...
2016: Scientific Reports
Takao Kamai, Hideyuki Abe, Kyoko Arai, Satoshi Murakami, Setsu Sakamoto, Yasushi Kaji, Ken-Ichiro Yoshida
BACKGROUND: Hereditary leiomyomatosis and renal cell carcinoma (HLRCC) is an autosomal dominant tumor susceptibility syndrome, and the disease-related gene has been identified as fumarate hydratase (fumarase, FH). HLRCC-associated kidney cancer is an aggressive tumor characterized by early metastasis to regional lymph nodes and distant organs. Since early diagnosis and provision of definitive therapy is thought to be the best way to reduce the tumor burden, it is widely accepted that germline testing and active surveillance for an at-risk individual from a family with HLRCC is very important...
2016: BMC Cancer
Elizabeth W Duggan, Matthew A Klopman, Arnold J Berry, Guillermo Umpierrez
Hyperglycemia is a frequent manifestation of critical and surgical illness, resulting from the acute metabolic and hormonal changes associated with the response to injury and stress (Umpierrez and Kitabchi, Curr Opin Endocrinol. 11:75-81, 2004; McCowen et al., Crit Care Clin. 17(1):107-24, 2001). The exact prevalence of hospital hyperglycemia is not known, but observational studies have reported a prevalence of hyperglycemia ranging from 32 to 60 % in community hospitals (Umpierrez et al., J Clin Endocrinol Metab...
March 2016: Current Diabetes Reports
Yang Lin, Eric A Lewallen, Emily T Camilleri, Carolina A Bonin, Dakota L Jones, Amel Dudakovic, Catalina Galeano-Garces, Wei Wang, Marcel J Karperien, A Noelle Larson, Diane L Dahm, Michael J Stuart, Bruce A Levy, Jay Smith, Daniel B Ryssman, Jennifer J Westendorf, Hee-Jeong Im, Andre J van Wijnen, Scott M Riester, Aaron J Krych
Preservation of osteochondral allografts used for transplantation is critical to ensure favorable outcomes for patients after surgical treatment of cartilage defects. To study the biological effects of protocols currently used for cartilage storage, we investigated differences in gene expression between stored allograft cartilage and fresh cartilage from living donors using high throughput molecular screening strategies. We applied next generation RNA sequencing (RNA-seq) and real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) to assess genome-wide differences in mRNA expression between stored allograft cartilage and fresh cartilage tissue from living donors...
February 22, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Irine Sidiropoulou, Georgia G Tsaousi, Chryssa Pourzitaki, Helen Logotheti, Dimitrios Tsantilas, Dimitrios G Vasilakos
The aim of this randomized, double-blind clinical trial was to elucidate the impact of general anesthesia alone (GA) or supplemented with epidural anesthesia (EpiGA) on surgical stress response during laparoscopic cholecystectomy, using stress hormones, glucose, and C-reactive protein (CRP), as potential markers. Sixty-two patients scheduled to undergo elective laparoscopic cholecystectomy were randomly assigned into two groups to receive either GA or EpiGA. Stress hormones [cortisol (COR), human growth hormone (hGH), prolactine (PRL)], glucose, and CRP were determined 1 day before surgery, intraoperatively, and upon first postoperative day (POD1)...
June 2016: Journal of Anesthesia
Rodrigo Rodrigues da Conceição, Roberto Laureano-Melo, Kelen Carneiro Oliveira, Maria Clara de Carvalho Melo, Tereza Sayoko Kasamatsu, Rui Monteiro de Barros Maciel, Janaina Sena de Souza, Gisele Giannocco
Thyroidectomy is a surgical procedure indicated in cases of several maligned or benign thyroid diseases, thus, the aim of our study was to verify how the hypothyroidism induced by thyroidectomy influences behavioral parameters and its relation to thyroid hormones metabolism and neurogenesis at hippocampus. For this purpose, Adult male Wistar rats underwent to thyroidectomy to induce hypothyroidism. Behavioral tests, the thyroid profile and hippocampal gene expression were evaluated in control and in thyroidectomized animals...
April 1, 2016: Physiology & Behavior
Birce Akpinar, Ethiene V Bracht, Dorin Reijnders, Barbora Safarikova, Iva Jelinkova, Alf Grandien, Alena Hyrslova Vaculova, Boris Zhivotovsky, Magnus Olsson
Despite recent advances in targeted therapeutics, administration of 5-fluorouracil (5-FU) remains a common clinical strategy for post-surgical treatment of solid tumors. Although it has been proposed that RNA metabolism is disturbed by 5-FU treatment, the key cytotoxic response is believed to be enzymatic inhibition of thymidylate synthase resulting in nucleotide pool disproportions. An operating p53 tumor suppressor signaling network is in many cases essential for the efficiency of chemotherapy, and malfunctions within this system remain a clinical obstacle...
December 22, 2015: Oncotarget
Shuqiang Yuan, Yunxin Lu, Jing Yang, Gang Chen, Sangbae Kim, Li Feng, Marcia Ogasawara, Naima Hammoudi, Weiqin Lu, Hui Zhang, Jinyun Liu, Howard Colman, Ju-Seog Lee, Xiao-Nan Li, Rui-Hua Xu, Peng Huang, Feng Wang
INTRODUCTION: Cancer stem cells (CSCs) possess characteristics associated with normal stem cells, specifically the abilities to renew themselves and to give rise to all cell types (differentiation). It is assumed that induction of differentiation in CSCs would reduce their ability to form tumors. What triggers CSC differentiation and the role of "differentiation" in tumorigenesis remain elusive. METHODS: Glioma stem cell (GSC) lines and subcutaneous as well as orthotopic xenografts established from fresh surgical specimens of glioblastoma multiforme were used...
2015: Stem Cell Research & Therapy
Vlad C Sandulache, Yunyun Chen, Heath D Skinner, Tongtong Lu, Lei Feng, Laurence E Court, Jeffrey N Myers, Raymond E Meyn, Clifton D Fuller, James A Bankson, Stephen Y Lai
Ionizing radiation is the primary nonsurgical treatment modality for solid tumors. Its effectiveness is impacted by temporal constraints such as fractionation, hypoxia, and development of radioresistant clones. Biomarkers of acute radiation response are essential to developing more effective clinical algorithms. We hypothesized that acute perturbations in tumor lactate levels act as a surrogate marker of radiation response. In vitro experiments were carried out using validated human-derived cell lines from three histologies: anaplastic thyroid carcinoma (ATC), head and neck squamous cell carcinoma (HNSCC), and papillary thyroid carcinoma (PTC)...
December 2015: Molecular Cancer Therapeutics
E G Gorozhanskaya, S P Sviridova, V N Baykova, G N Zubrikhina, M M Dobrovolskaya, A V Sitov
To determine the biochemical disorders in the blood coagulation mechanism associated with oxidative stress parameters of the antioxidant status were examined in platelets of 57 colorectal cancer patients, (including 21 patients before and after surgery), and 40 healthy individuals. We determined the total content of nitric oxide (NOx), levels of superoxide dismutase (Cu/ZnSOD), glutathione and malondialdehyde (MDA). Before treatment, we observed the changes in the antioxidant defense system of platelets, which did not depend on the prevalence of malignancy: elevated levels of SOD by 16% (p<0...
July 2015: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
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