Pietro Ancona, Alessandro Trentini, Anna Terrazzan, Silvia Grassilli, Pauline Navals, Eric W J Gates, Valentina Rosta, Carlo Cervellati, Carlo M Bergamini, Angela Pignatelli, Jeffrey W Keillor, Cristian Taccioli, Nicoletta Bianchi
Transglutaminase 2 (TG2) performs many functions both under physiological and pathological conditions. In cancer, its expression is associated with aggressiveness, propensity to epithelial-mesenchymal transition, and metastasis. Since TG2 performs key functions both outside and inside the cell, using inhibitors with different membrane permeability we analyzed the changes in the transcriptome induced in two triple-negative cell lines (MDA-MB-436 and MDA-MB-231) with aggressive features. By characterizing pathways and gene networks, we were able to define the effects of TG2 inhibitors (AA9, membrane-permeable, and NCEG2, impermeable) in relation to the roles of the enzyme in the intra- and extracellular space within the context of breast cancer...
April 9, 2024: Journal of Molecular Biology