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Michaela Rýdlová, Karolína Růnová, Jiřina Száková, Alena Fučíková, Anna Hakenová, Petr Mlejnek, Václav Zídek, Jana Tremlová, Oto Mestek, Antonín Kaňa, Jarmila Zídková, Magdalena Melčová, Klára Truhlářová, Pavel Tlustoš
The response of nutrient status and biochemical processes in (i) Wistar and (ii) spontaneously hypertensive (SHR) rats upon dietary intake of selenium- (Se-) enriched defatted rapeseed (DRS) and/or vitamin E fortification was examined to assess the health benefit of DRS in animal nutrition. Twenty-four individuals of each type of rat were used: The control group was fed with an untreated diet (Diet A). In Diets B and C, soybean meal was replaced with defatted DRS, which comprised 14% of the total diet. The selenized DRS application resulted in ~3-fold increase of Se content in the diet...
2017: BioMed Research International
Shengmin Zhou, Yueqiang Wang, Yuanrong Jiang, Zhongfei Zhang, Xiangjun Sun, Liangli Lucy Yu
Three medium- and long-chain triacylglycerols (MLCT) with different contents of medium-chain fatty acids (MCFA) (10% to 30%, w/w) were prepared and evaluated for their anti-obesity potential in C57BL/6J mice. The group fed with a high fat diet of MLCT containing 30% (w/w) MCFA showed significantly decreased body weight and fat mass (P < 0.05) relative to the control mice fed an obesity-inducing high fat rapeseed oil diet. In addition, serum parameters including triacylglycerols, total cholesterol, glucose, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, apolipoprotein A1 and apolipoprotein B in the treatment group fed with 30% (w/w) MCFA were close to those of mice fed with a low fat rapeseed oil diet, but significantly different (P < 0...
June 20, 2017: Journal of Food Science
Kai F Pfister, Sabrina Baader, Mathias Baader, Silvia Berndt, Lukas J Goossen
Rapeseed oil methyl ester (RME) and (bio)ethylene are converted into biofuel with an evenly rising boiling point curve, which fulfills the strict boiling specifications prescribed by the fuel standard EN 590 for modern (petro)diesel engines. Catalyzed by a Pd/Ru system, RME undergoes isomerizing metathesis in a stream of ethylene gas, leading to a defined olefin, monoester, and diester blend. This innovative refining concept requires negligible energy input (60°C) and no solvents and does not produce waste...
June 2017: Science Advances
C F Visscher, A Abd El-Wahab, M F E Ahmed, J Hankel, V Taube, J Kamphues
The aim of the present study was to examine possible effects of a different protein supply on the presence of Campylobacter in excreta and caecal content of broiler chickens. Therefore, 220 one-day-old chickens were fed five different diets in the experimental period (day 8 onwards). In the control group (CON; n = 3 boxes), a soya bean-based industrially produced complete diet containing 5-10% whole wheat and coccidiostats was fed, whereas in the remaining groups, four own-made complete diets with four protein sources and combinations (4 × 2 boxes; soya bean meal-based diet = SBM; rapeseed meal-containing diet = RSM; haemoglobin meal-containing diet = HGP; algae-containing diet = ALG) were offered...
June 2017: Journal of Animal Physiology and Animal Nutrition
Ping Xu, Shiqin Cao, Kaining Hu, Xiaohua Wang, Wei Huang, Gang Wang, Zewen Lv, Zhongsong Liu, Jing Wen, Bin Yi, Chaozhi Ma, Jinxing Tu, Tingdong Fu, Jinxiong Shen
As a desirable agricultural trait, multilocular trait of rapeseed (Brassica rapa; Brassica napus; Brassica juncea), always represents higher yield per plant compared with bilocular plants. We previously isolated a trilocular gene locus, Bjmc1, and identified a set of molecular markers linked to the trilocular gene. With a map-based cloning, we identified that the BjMc1 was located in B genome of Brassica juncea, and it was a CLAVATA1 (CLV1) gene homologue. The insertion of a copia-LTR retrotransposable element 1 (RTE1) into the coding region of BjMc1 interrupted its transcription in rapeseed, leading to the trilocular phenotype...
June 14, 2017: Scientific Reports
Bi-Yan Zhao, Yu-Feng Hu, Juan-Juan Li, Xuan Yao, Ke-de Liu
BACKGROUND: Abiotic stresses such as drought and salt stresses have a negative effect on the growth and productivity of plants. Improvement of stress tolerance through genetic engineering in plants has been reported in intense studies. Transcription factors play vital roles in plant adaptation to stresses by regulating expression of a great deal of target genes. A family of Arabidopsis basic region leucine zipper (bZIP) transcription factors that can recognize and bind to the abscisic acid (ABA)-responsive elements (ABREs) in promoter is named as ABRE binding factors (ABFs)/ABRE binding proteins (AREBs)...
December 2016: Botanical Studies (Taipei, Taiwan)
Ke-Lin Huang, Mei-Li Zhang, Guang-Jing Ma, Huan Wu, Xiao-Ming Wu, Feng Ren, Xue-Bao Li
Seed oil content is an important agronomic trait in oilseed rape. However, the molecular mechanism of oil accumulation in rapeseeds is unclear so far. In this report, RNA sequencing technique (RNA-Seq) was performed to explore differentially expressed genes in siliques of two Brassica napus lines (HFA and LFA which contain high and low oil contents in seeds, respectively) at 15 and 25 days after pollination (DAP). The RNA-Seq results showed that 65746 and 66033 genes were detected in siliques of low oil content line at 15 and 25 DAP, and 65236 and 65211 genes were detected in siliques of high oil content line at 15 and 25 DAP, respectively...
2017: PloS One
Qin Wang, Hui Zheng, Xia Wan, Hongfeng Huang, Junhui Li, Christopher T Nomura, Changjun Wang, Shouwen Chen
Bacitracin is a broad-spectrum antibiotic used extensively as a feed additive. In this study, inexpensive agricultural by-products were used as nitrogen sources for bacitracin production. Based on both the orthogonal tests, a combination of 7% soybean meal (SBM) +2% low protein rapeseed cake (LPRC) was optimal for bacitracin production. Compared to the original formula, the titer of bacitracin increased by 20.5% reaching 910.4 U/ml in flasks. The titer of bacitracin and the ratio of bacitracin A increased by 12...
June 7, 2017: Applied Biochemistry and Biotechnology
Ke-Jing An, Yu-Lan Liu, Hai-Lan Liu
Deep-fried dough sticks (a Chinese traditional breakfast) were fried individually in peanut, sunflower, rapeseed, rice bran, soybean and palm oil without any time lag for 32 h (64 batches fried, each for 30 min) and fried oil samples were obtained every 2 h. The frying-induced changes in the levels of total polar compounds (TPC) and polycyclic aromatic hydrocarbons (PAHs) were investigated by edible oil polar compounds (EOPC) fast separation chromatographic system and gas chromatography-mass spectrometry (GC-MS), respectively...
June 20, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Janina Braatz, Hans-Joachim Harloff, Martin Mascher, Nils Stein, Axel Himmelbach, Christian Jung
In polyploid species, altering a trait by random mutagenesis is highly inefficient due to gene redundancy. We have stably transformed tetraploid oilseed rape (Brassica napus) with a CRISPR-Cas9 construct targeting two ALCATRAZ (ALC) homoeologs. ALC is involved in valve margin development and, thus, contributes to seed shattering from mature fruits. Knocking out ALC would increase shatter resistance to avoid seed loss during mechanical harvest. We obtained a transgenic T1 plant with four alc mutant alleles by the use of a single target sequence...
June 2017: Plant Physiology
R Sandeep Raj, Sonal V Thakur, Vora Shebhan Hussen, Madhvi N Joshi, Shradha Nand Tyagi, Snehal B Bagatharia
The exorbitant yield loss incurred by Indian farmers every year (10-90%) in rapeseed-mustard (Brassica juncea) is chiefly attributed to the progressive infestation of mustard fields by Lipaphis erysimi (Kalt.), a major insect pest belonging to the family of Homoptera. Currently there are no successful tolerant cultivars developed by conventional means in Brassica juncea with systemic plant responses in the form of direct or indirect defenses against aphid attack. Lack of specific methods for screening large numbers of genotypes required in breeding for selection of tolerant cultivars in mustard is one of the main causes of slow progress in developing resistant varieties of Brassica juncea...
June 2017: 3 Biotech
Regina Huber, Regine Schoenlechner
Fresh egg waffles are continuously baked in tunnel baking ovens in industrial scale. Waffles that partly or fully stick to the baking plates cause significant product loss and increased costs. The aim of this study was, therefore, to investigate the effect of different recipe ingredients on the sticking behavior of waffles. In this second part, ingredients investigated were different leavening agents (sodium acid pyrophosphate, ammonium bicarbonate, magnesium hydroxide carbonate, or monocalcium phosphate), different fat sources (rapeseed oil, cocos fat, butter, or margarine), and different water sources (tap water 12°dH and distilled water)...
May 2017: Food Science & Nutrition
Yoko Kawamura, Yuko Ogawa, Motoh Mutsuga
Nonylphenol (NP) has been suspected as an endocrine-disrupting chemical. Japanese polyvinyl chloride (PVC) stretch films contained 0.5-3.3 mg/g of NP and 100-400 mg/g of plasticizers such as diisononyl adipate (DINA), di-n-alkyl adipate (DAA), and diacetyllauroyl glycerol (DALG). Migration of NP and plasticizers from PVC stretch films into food simulants (water; 4% acetic acid; 20%, 50%, and 95% ethanol; and heptane), rapeseed oil, and foods was investigated. Plasticizers migrated only in small amounts into aqueous simulants and foods, although they migrated at much higher levels into 50% and 95% ethanol, heptane, rapeseed oil, and fatty foods, whereas NP more easily migrated into aqueous simulants and foods...
May 2017: Food Science & Nutrition
Peifa Liu, Yusheng Zhao, Guozheng Liu, Meng Wang, Dandan Hu, Jun Hu, Jinling Meng, Jochen C Reif, Jun Zou
Genomics-based prediction of hybrid performance promises to boost selection gain. The main goal of our study was to investigate the relevance of additive, dominance, and epistatic effects for determining hybrid seed yield in a biparental rapeseed population. We re-analyzed 60,000 SNP array and seed yield data points from an immortalized F2 population comprised of 318 hybrids and 180 parental lines by performing genome-wide QTL mapping and predictions in combination with five-fold cross-validation. Moreover, an additional set of 37 hybrids were genotyped and phenotyped in an independent environment...
2017: Frontiers in Plant Science
Quan Zhang, Haifei Chen, Mingliang He, Zhuqing Zhao, Hongmei Cai, Guangda Ding, Lei Shi, Fangsen Xu
Boron (B) is an essential micronutrient for plants, but the molecular mechanisms underlying the uptake and distribution of B in allotetraploid rapeseed (Brassica napus) are unclear. Here, we identified a B transporter of rapeseed, BnaC4.BOR1;1c, which expressed in shoot nodes and involved in distributing B to the reproductive organs. Transgenic Arabidopsis plants containing a BnaC4.BOR1;1c promoter-driven GUS reporter gene showed strong GUS activity in roots, nodal regions of the shoots and immature floral buds...
May 25, 2017: Plant, Cell & Environment
Stanisława Raj, Grzegorz Skiba, Monika Sobol, Barbara Pastuszewska
The effect of dietary fats differing in fatty acid (FA) composition on the metabolism of saturated FA (SFA) and monounsaturated FA (MUFA) in growing pigs was investigated. The deposition of FA in the body and the fate of individual dietary FA were assessed after slaughter. Gilts with an initial body weight (BW) of 60 kg were used as experimental animals. Six pigs were slaughtered at 60 kg BW, while further 18 pigs received three isoenergetic and isonitrogen experimental diets containing linseed oil, rapeseed oil or beef tallow at 50 g/kg diet until they reached 105 kg (six pigs per group)...
May 25, 2017: Archives of Animal Nutrition
Yu Liang, Neng Wan, Zao Cheng, Yufeng Mo, Baolin Liu, Hui Liu, Nadia Raboanatahiry, Yongtai Yin, Maoteng Li
Vicinal oxygen chelate proteins (VOC) are members of the metalloenzyme superfamily, which plays roles in many biological reactions. Some members of the VOC superfamily have been systematically characterized but not in Brassica napus. In this study, 38 VOC genes were identified based on their conserved domains. The present results revealed that most of the BnaVOC genes have few introns, and all contained the typical VOC structure of βαβββ modules. The BnaVOC genes are distributed unevenly across 15 chromosomes in B...
2017: Frontiers in Plant Science
Shahbaz Khan, Sumera Anwar, Jie Kuai, Sana Ullah, Shah Fahad, Guangsheng Zhou
Yield and lodging related traits are essential for improving rapeseed production. The objective of the present study was to investigate the influence of plant density (D) and nitrogen (N) rates on morphological and physiological traits related to yield and lodging in rapeseed. We evaluated Huayouza 9 for two consecutive growing seasons (2014-2016) under three plant densities (LD, 10 plants m(-2); MD, 30 plants m(-2); HD, 60 plants m(-2)) and four N rates (0, 60, 120, and 180 kg ha(-1)). Experiment was laid out in split plot design using density as a main factor and N as sub-plot factor with three replications each...
2017: Frontiers in Plant Science
Yamin Cui, Pengfei Hao, Bingjie Liu, Xianghong Meng
The effect of four frying processes (vegetable salad, stir frying, pan frying, and deep frying) on fatty acid composition of ten vegetable oils (peanut oil, soybean oil, rapeseed oil, corn oil, sunflower seed oil, rice bran oil, olive oil, sesame oil, linseed oil, and peony seed oil) was investigated using GC-MS. The result showed that trans-fatty acid (TFA) was produced during all processes. Rapeseed oil had the highest TFA content in vegetable salad oil with 2.88% of total fatty acid. The TFA content of sunflower seed oil was 0...
October 15, 2017: Food Chemistry
Dongxian Zhang, Jianfei Zhu, Fayin Ye, Guohua Zhao
The sorption capacity of arabinan-rich pectic polysaccharide (ARPP) onto ferulic acid (FA) was investigated using equilibrium dialysis assays. UV and FT-IR spectra showed that FA was successfully adsorbed by ARPP. The effects of temperature, pH, buffer concentration, NaCl, and ethanol on sorption were investigated. Sorption variable optimization was examined by response surface methodology. The order of influence of each factor in affecting the sorption capacity was temperature>pH>buffer concentration...
May 15, 2017: International Journal of Biological Macromolecules
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