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Milda Šulcienė, Boris Kolvenbach, Erik Ammann, Inga Matijošytė
The production of biopolyols (sustainable, renewable and biodegradable precursors), is exclusively focused on the conventional chemical synthesis pathways, and currently, no polyols are produced by enzymatic processes at an industrial scale, because they are not competitive when compared to petrochemically based polyols due to the overall cost for their production. This can be mainly attributed to the high costs of commercial enzymes used in this process and their use is limited by their lack of stability during the bioprocess...
May 16, 2018: International Journal of Biological Macromolecules
Harri Juhani Saarinen, Sari Husgafvel, Hanna Pohjantähti-Maaroos, Marja Wallenius, Ari Palomäki
Background: The aim of this study was to compare acute effects of turnip rapeseed oil rich with mono- and polyunsaturated fatty acids and cream on postprandial triglyceride levels and post-glucose load measures of insulin sensitivity in population of men with metabolic syndrome. Methods: This open-label balanced crossover study included 37 men with metabolic syndrome. They underwent an oral glucose-fat tolerance test where they ingested 75 g of glucose with either 240 mL of cream or 84 mL of turnip rapeseed oil depending on the study arm...
2018: Diabetology & Metabolic Syndrome
Johanna Karlsson, Rolf Spörndly, Mikaela Lindberg, Kjell Holtenius
Global demand is increasing for food, and use of large amounts of potentially human-edible feedstuffs for dairy cows is an important concern. The present study examined whether feeding a by-product-based concentrate combined with high-quality grass silage to high-producing dairy cows affected feed intake and milk production compared with a conventional diet, as well as the effect on efficiency of human food production. In a changeover experiment with four 21-d periods, 24 dairy cows in mid-lactation were offered 9...
May 9, 2018: Journal of Dairy Science
Cylia Haddad, Mustapha Arkoun, Franck Jamois, Adrian Schwarzenberg, Jean-Claude Yvin, Philippe Etienne, Philippe Laîné
Silicon (Si) is the second most abundant element in soil and has several beneficial effects, especially in plants subjected to stress conditions. However, the effect of Si in preventing nitrogen (N) starvation in plants is poorly documented. The aim of this work was to study the effect of a short Si supply duration (7 days) on growth, N uptake, photosynthetic activity, and leaf senescence progression in rapeseed subjected (or not) to N starvation. Our results showed that after 1 week of Si supply, Si improves biomass and increases N uptake and root expression of a nitrate transporter gene...
2018: Frontiers in Plant Science
JiSu Kim, Mi-Ja Kim, JaeHwan Lee
Effects of different moisture contents and oxidised compounds on the critical micelle concentration (CMC) of lecithin were determined in bulk oils and in medium-chain triacylglycerols (MCT). CMC of lecithin in MCT was significantly higher than that in other vegetable oils including olive, soybean, corn, and rapeseed oils (p < 0.05). Presence of moisture significantly affected the CMC of lecithin in MCT (p < 0.05). CMC of lecithin was high when the moisture content was below 900 ppm, whereas at a moisture content of 1000 ppm, CMC of lecithin decreased significantly (p < 0...
September 30, 2018: Food Chemistry
Islam Muhammad Shariful, Nakako Katsuno, Takahisa Nishizu
Kinetic analysis was used to study the destabilization of mayonnaise by focusing on the fat crystals. Mayonnaise prepared from rapeseed oil and soybean oil was stored at temperatures ranging from &minus;20 to &minus;40 &deg;C. The destabilization kinetic parameters were measured by observing oil separation over time. The destabilization rate constant, kd , increased with decreasing temperature. The highest value of kd was 1.28 &times; 10&minus;3 min&minus;1 at &minus;40 &deg;C for rapeseed oil mayonnaise (RoM) and the lowest was 1...
May 8, 2018: Foods (Basel, Switzerland)
Shi Shen, Jingbo Wang, Qin Zhuo, Xi Chen, Tingting Liu, Shuang-Qing Zhang
Phenolics and flavonoids in honey are considered as the main phytonutrients which not only act as natural antioxidants, but can also be used as floral markers for honey identification. In this study, the chemical profiles of phenolics and flavonoids, antioxidant competences including total phenolic content, DPPH and ABTS assays and discrimination using chemometric analysis of various Chinese monofloral honeys from six botanical origins (acacia, Vitex , linden, rapeseed, Astragalus and Codonopsis ) were examined...
May 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Feiran Xu, Jing Zhang, Zhigao Wang, Yijun Yao, Griffiths G Atungulu, Xingrong Ju, Lifeng Wang
WDHHAPQLR (RAP), an anti-oxidative peptide derived from rapeseed protein. Although the health benefits from RAP due to their antioxidant activities have been determined by chemical methods, a systematic assessment regarding the absorption, metabolism and antioxidation process of RAP is still lacking attention. Hence, Caco-2 cell monolayers model and animal experiment were used to evaluate the absorption and bioavailability of RAP. As expected, RAP could be absorbed by intestinal epithelial cell, and the Papp was (0...
May 7, 2018: Journal of Agricultural and Food Chemistry
Chao Li, Mengyu Hao, Wenxiang Wang, Hui Wang, Fan Chen, Wen Chu, Baohong Zhang, Desheng Mei, Hongtao Cheng, Qiong Hu
With the rapid development of sequence specific nucleases (SSNs) for genome targeting, clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) is now considered the most promising method for functional genetic researches, as well as genetic improvement in crop plants. However, the gene redundancy feature within the allotetraploid rapeseed genome is one of the major obstacles for simultaneous modification of different homologs in the first generation. In addition, large scale screening to identify mutated transgenic plants is very time-and labor-consuming using the conventional restriction enzyme-based approaches...
2018: Frontiers in Plant Science
Lunlin Chen, Heping Wan, Jiali Qian, Jianbin Guo, Chengming Sun, Jing Wen, Bin Yi, Chaozhi Ma, Jinxing Tu, Laiqiang Song, Tingdong Fu, Jinxiong Shen
Cadmium is a potentially toxic heavy metal to human health. Rapeseed ( Brassica napus L.), a vegetable and oilseed crop, might also be a Cd hyperaccumulator, but there is little information on this trait in rapeseed. We evaluated Cd accumulation in different oilseed accessions and employed a genome-wide association study to identify quantitative trait loci (QTLs) related to Cd accumulation. A total of 419 B. napus accessions and inbred lines were genotyped with a 60K Illumina Infinium SNP array of Brassica...
2018: Frontiers in Plant Science
Hongfei Gao, Luke Wen, Yuhua Wu, Xiaohong Yan, Jun Li, Xiaofei Li, Zhifeng Fu, Gang Wu
A highly sensitive electrochemiluminescent (ECL) immunoassay targeting PAT/bar protein was facilely developed for genetically modified (GM) rapeseed detection using carbon nanoparticles (CNPs) originally prepared from printer toner. In this work, CNPs linked with antibody for PAT/bar protein were used to modify a working electrode. After an immunoreaction between the PAT/bar protein and its antibody, the immunocomplex formed on the electrode receptor region resulted in an inhibition of electron transfer between the electrode surface and the ECL substance, thus led to a decrease of ECL response...
May 2, 2018: Journal of Agricultural and Food Chemistry
Martin Musil, Frantisek Skopal, Martin Hájek, Ales Vavra
Biodiesel is a mixture of esters of fatty acids (most often palmitic, stearic and oleic) and lower alcohols (in our work butanol) produced by transesterification. It is a renewable source of energy, prepared from triacylglycerides, which are contained in vegetable oils and animal fats. This work focuses on alkaline catalyzed transesterification of rapeseed oil with butanol and comparison of two catalysts (potassium hydroxide and potassium tert-butoxide). In industry is usually transesterification of rapeseed oil carried out like reaction catalyzed by potassium hydroxide...
April 27, 2018: Journal of Environmental Management
Xian Duo Zhang, Kai Xuan Zhao, Zhi Min Yang
The plant ATP binding cassette (ABC) transporters are one of the integral membrane proteins responsible for uptake and allocation of a wide range of metabolites and xenobiotics including heavy metals (e.g. zinc, manganese and cadmium). They play multiple roles in plant growth, development and environmental stress responses. Although the ABC transporters have been identified in model plants such as Arabidopsis and rice, they are have not been annotated and identified in rapeseed (Brassica napus) and also, little is known about functionality of these metal transporters...
April 27, 2018: Gene
Yongtai Yin, Liangxing Guo, Kang Chen, Zhenyi Guo, Hongbo Chao, Baoshan Wang, Maoteng Li
Rapeseed is one of the most important and widely cultured oilseed crops for food and nonfood purposes worldwide. Neutral lipids are stored in lipid droplets (LDs) as fuel for germination and subsequent seedling growth. Most of the LD detection in seeds was still in 2D levels, and some of the details might have been lost in previous studies. In the present work, the configuration of LDs in seeds was obtained by confocal imaging combined with 3D reconstruction technology in Brassica napus. The size and shape of LDs, LD numbers, cell interval spaces and cell size were observed and compared at 3D levels in the seeds of different materials with high and low oil content...
April 26, 2018: Scientific Reports
Ewelina Pogorzelska-Nowicka, Jolanta Godziszewska, Jarosław O Horbańczuk, Atanas G Atanasov, Agnieszka Wierzbicka
The study evaluated the effect of pig diet supplementation with rapeseed or linseed oil, and vitamin E or selenium, or both vitamin E and selenium on color parameters and myoglobin content of pork Semimembranosus muscle after long-term freezing storage during nine months. The influence of the type of the bioactive compounds added to pig diet on the content of myoglobin or oxymyoglobin, metmyoglobin and deoksymyoglobin in Semimembranosus m. was also assessed. The results indicate that the presence of oils rich in polyunsaturated fatty acids (PUFA) in pig diet improves the color of pork meat...
April 25, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Hui Han, Qi Wang, Lin-Yan He, Xia-Fang Sheng
Two Cd-immobilizing and polyamine-producing bacteria Serratia liquefaciens CL-1 and Bacillus thuringiensis X30 were characterized for their effects on Cd immobilization, pH, and polyamine production in the solution and the rapeseed biomass and Cd uptake of Brassica napus Qinyou-10 in Cd-contaminated soil. These strains significantly increased pH and reduced water-soluble Cd concentration (25-76%) compared to the controls. Furthermore, strain CL-1 produced more polyamine (71-192%) in the solution than strain X30...
April 14, 2018: Journal of Hazardous Materials
Miloš Skřivan, Milan Marounek, Michaela Englmaierová, Ladislav Čermák, Jana Vlčková, Eva Skřivanová
A group of 240-day-old Ross cockerels were used in a 4-week experiment to assess the effect of the fat type on the intestinal digestibility of fatty acids (FAs), the FA profiles of breast meat and abdominal fat, and the mRNA expression of six hepatic lipid-related genes. Experimental diets were supplemented with rapeseed oil, pork lard or palm oil at 60 g/kg. In the control diet, wheat starch was substituted for the fat source. The highest ileal digestibility of the fat and all FAs (except stearic acid) was observed in chickens fed lard...
2018: PloS One
Sarah Hatzig, Frank Breuer, Nathalie Nesi, Sylvie Ducournau, Marie-Helene Wagner, Gunhild Leckband, Amine Abbadi, Rod J Snowdon
Intense selection for specific seed qualities in winter oilseed rape breeding has had an inadvertent negative influence on seed germination performance. In a panel of 215 diverse winter oilseed rape varieties spanning over 50 years of breeding progress in winter-type rapeseed, we found that low seed erucic acid content and reduced seed glucosinolate content were significantly related with prolonged germination time. Genome-wide association mapping revealed that this relationship is caused by linkage drag between important loci for seed quality and germination traits...
2018: Frontiers in Plant Science
Tao Luo, Jing Zhang, Mohammad Nauman Khan, Jiahuan Liu, Zhenghua Xu, Liyong Hu
To understand the influence of temperature on floral initiation and to reveal the relationship between floral bud development and yield potential of rapeseed (Brassica napus L.), early- ("1358"), intermediate- ("Zhongshuang No.11") and late- ("Zheshuang No.8") maturity genotypes were sown on different sowing dates under field conditions during four crop seasons. A multiplicative model was introduced to distinguish and quantify the effects of photoperiod and temperature on pre-floral initiation phase...
June 2018: Plant Science: An International Journal of Experimental Plant Biology
M Kaci, A Belhaffef, S Meziane, G Dostert, P Menu, É Velot, S Desobry, E Arab-Tehrany
Emulsion-based delivery systems have been developed to increase the topical bioavailability of lipophilic active compounds within skin membrane. The aim was to develop nanoemulsion from natural sources (rapeseed oil) with the same sources of pure phospholipids (lecithin) rich on mono and polyunsaturated fatty acids for encapsulation of hydrophobic antioxidant (Coenzyme Q10 ) giving nanoemulsion with double functionality. Nanoemulsions were used for cream preparation using xanthan gum and carboxylmethylcellulose as texturizing agents...
April 5, 2018: Colloids and Surfaces. B, Biointerfaces
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