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https://www.readbyqxmd.com/read/28822264/trimethylamine-n-oxide-as-a-risk-factor-for-atherosclerosis-induces-stress-in-j774a-1-murine-macrophages
#1
Abbas Mohammadi, Zakaria Vahabzadeh, Soran Jamalzadeh, Tahereh Khalili
PURPOSE: Trimethylamine N-oxide (TMAO) is a biomarker for kidney problems. It has also been introduced as a risk factor for atherosclerosis. The classic risk factors for atherosclerosis trigger cellular and humeral immunoreaction in macrophages through induction of heat shock protein expressions and increased levels of GRP94 and HSP70 are associated with increased atherosclerosis risk. The present study evaluated the possible effect(s) of TMAO on the expression of GRP94 and HSP70 at protein levels...
August 16, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28821885/the-expression-profiles-of-mirna-mrna-of-early-response-in-genetically-improved-farmed-tilapia-oreochromis-niloticus-liver-by-acute-heat-stress
#2
Jun Qiang, Wen J Bao, Fan Y Tao, Jie He, Xia H Li, Pao Xu, Lan Y Sun
Genetically improved farmed tilapia (GIFT, Oreochromis niloticus) are commercially important fish that are cultured in China. GIFT are highly susceptible to diseases when exposed to high temperatures in summer. Better understanding the GIFT regulatory response to heat stress will not only help in determining the relationship between heat stress signalling pathways and adaption mechanisms, but will also contribute to breeding new high-temperature tolerant strains of GIFT. In this study, we built control (28 °C) and heat-treated (37...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821876/transcriptomic-biochemical-and-physio-anatomical-investigations-shed-more-light-on-responses-to-drought-stress-in-two-contrasting-sesame-genotypes
#3
Komivi Dossa, Donghua Li, Linhai Wang, Xiaomin Zheng, Aili Liu, Jingyin Yu, Xin Wei, Rong Zhou, Daniel Fonceka, Diaga Diouf, Boshou Liao, Ndiaga Cissé, Xiurong Zhang
Sesame is an important oilseed crop with a high oil quality. It is prone to drought stress in the arid and semi-arid areas where it is widely grown. This study aims to decipher the response of tolerant (DT) and sensitive (DS) genotypes to progressive drought based on transcriptome, biochemical and physio-anatomical characterizations. Results indicated that under severe stress, DT relied on a well-functioning taproot while DS displayed a disintegrated root due to collapsed cortical cells. This was attributed to a higher accumulation of osmoprotectants and strong activity of antioxidant enzymes especially peroxidases in DT...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821863/the-heat-shock-response-and-humoral-immune-response-are-mutually-antagonistic-in-honey-bees
#4
Mia McKinstry, Charlie Chung, Henry Truong, Brittany A Johnston, Jonathan W Snow
The honey bee is of paramount importance to humans in both agricultural and ecological settings. Honey bee colonies have suffered from increased attrition in recent years, stemming from complex interacting stresses. Defining common cellular stress responses elicited by these stressors represents a key step in understanding potential synergies. The proteostasis network is a highly conserved network of cellular stress responses involved in maintaining the homeostasis of protein production and function. Here, we have characterized the Heat Shock Response (HSR), one branch of this network, and found that its core components are conserved...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819820/genome-wide-characterization-of-the-wrky-gene-family-in-radish-raphanus-sativus-l-reveals-its-critical-functions-under-different-abiotic-stresses
#5
Bernard Kinuthia Karanja, Lianxue Fan, Liang Xu, Yan Wang, Xianwen Zhu, Mingjia Tang, Ronghua Wang, Fei Zhang, Everlyne M'mbone Muleke, Liwang Liu
The radish WRKY gene family was genome-widely identified and played critical roles in response to multiple abiotic stresses. The WRKY is among the largest transcription factors (TFs) associated with multiple biological activities for plant survival, including control response mechanisms against abiotic stresses such as heat, salinity, and heavy metals. Radish is an important root vegetable crop and therefore characterization and expression pattern investigation of WRKY transcription factors in radish is imperative...
August 17, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28819216/identification-of-endogenous-reference-genes-for-rt-qpcr-expression-analysis-in-urochloa-brizantha-under-abiotic-stresses
#6
Luciana Midori Takamori, Alyne Valéria Carrion Pereira, Gustavo Maia Souza, Luiz Gonzaga Esteves Vieira, Alessandra Ferreira Ribas
Urochloa brizantha is one of the most important warm season forage grasses in tropical countries. Despite its importance, there are few studies on gene expression in this species under stressful conditions. Real-time (RT-qPCR) is an accurate technique for gene quantification analysis, but reference genes must be validated under the same conditions used to assess the expression of the target genes. Here, we evaluated the stability of nine reference genes: Actin 12, Eukaryotic initiation factor 4 A, Elongation factor-1 alpha, FTSH protease 4, U2 auxiliary fator, Succinol Co-enzyme A, Tubulin alfa-5, Tubulin beta-6, Ubiquitin conjugating enzyme...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28817717/short-report-trpv1-polymorphism-1911-a-g-alters-capsaicin-induced-sensory-changes-in-healthy-subjects
#7
Julia Forstenpointner, Matti Förster, Denisa May, Friederike Hofschulte, Ingolf Cascorbi, Gunnar Wasner, Janne Gierthmühlen, Ralf Baron
BACKGROUND: C-fibers express transient receptor potential (TRP) channels. These high-voltage gated channels function as integrators of different physical stresses (e.g. heat, protons, ATP). Additionally channel activation can be induced by capsaicin. Topically applied, capsaicin elicits burning pain, heat and mechanical hyperalgesia and serves as a human surrogate model for pain. It was suggested that the TRPV1-variant rs8065080 (1911A>G) plays a pivotal role in patients with neuropathic pain syndromes...
2017: PloS One
https://www.readbyqxmd.com/read/28816521/plume-activity-and-tidal-deformation-on-enceladus-influenced-by-faults-and-variable-ice-shell-thickness
#8
Marie Běhounková, Ondřej Souček, Jaroslav Hron, Ondřej Čadek
We investigated the effect of variations in ice shell thickness and of the tiger stripe fractures crossing Enceladus' south polar terrain on the moon's tidal deformation by performing finite element calculations in three-dimensional geometry. The combination of thinning in the polar region and the presence of faults has a synergistic effect that leads to an increase of both the displacement and stress in the south polar terrain by an order of magnitude compared to that of the traditional model with a uniform shell thickness and without faults...
August 17, 2017: Astrobiology
https://www.readbyqxmd.com/read/28814770/rapid-phenotypic-individualization-of-bacterial-sister-cells
#9
Sander K Govers, Antoine Adam, Hendrik Blockeel, Abram Aertsen
A growing bacterium typically divides into two genetically identical and morphologically similar sister cells and eventually gives rise to a clonal population. Nevertheless, significant phenotypic differentiation among isogenic cells frequently occurs, with the resulting heterogeneity in cellular behavior often ensuring population level growth and survival in complex and unpredictable environments. Although several mechanisms underlying the generation of phenotypic heterogeneity have been elucidated, the speed with which identical sister cells tend to phenotypically diverge from each other has so far remained unaddressed...
August 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28812967/tanshinone-induced-ers-suppresses-igfii-activation-to-alleviate-ang-ii-mediated-cardiac-hypertrophy
#10
Ya-Fang Chen, Nien-Hung Lee, Pei-Ying Pai, Li-Chin Chung, Chia-Yao Shen, Peramaiyan Rajendran, Yu-Feng Chen, Ray-Jade Chen, Vijaya Padma Viswanadha, Wei-Wen Kuo, Chih-Yang Huang
Cardiomyopathy involves changes in myocardial ultrastructure and cardiac hypertrophy. Angiotensin II (AngII) has previously been shown to stimulate the expression of IGF-2 and IGF-2R in H9c2 cardiomyoblasts and increase of blood pressure, and cardiac hypertrophy. Estrogen receptors (ERs) exert protective effects, such as anti-hypertrophy in cadiomyocytes. Tanshinone IIA (TSN), a main active ingredient from a Chinese medical herb, Salvia miltiorrhiza Bunge (Danshen), was shown to protect cardiomyocytes hypertrophy by different stress signals...
October 2017: Journal of Receptor and Signal Transduction Research
https://www.readbyqxmd.com/read/28812868/light-and-strong-hierarchical-porous-sic-foam-for-efficient-electromagnetic-interference-shielding-and-thermal-insulation-at-elevated-temperatures
#11
Caiyun Liang, Zhenfeng Wang, Lina Wu, Xiaochen Zhang, Huan Wang, Zhijiang Wang
A novel light but strong SiC foam with hierarchical porous architecture are fabricated by using dough as raw material via carbonization and followed carbothermal reduction with silicon source. A significant synergistic effect is achieved by embedding meso and nano pores in micro-sized porous skeleton, which endows the SiC foam with high-performance electromagnetic interference shielding (EMI), thermal insulation and mechanical properties. The micro-sized skeleton withstands the high stress. The meso- and nano-sized pores enhance the multiple reflection of the incident electromagnetic waves and elongate the path of heat transfer...
August 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28812766/modulatory-effects-of-arginine-glutamine-and-branched-chain-amino-acids-on-heat-shock-proteins-immunity-and-antioxidant-response-in-exercised-rats
#12
Carolina Soares Moura, Pablo Christiano Barboza Lollo, Priscila Neder Morato, Eder Muller Risso, Jaime Amaya-Farfan
Heat shock proteins (HSPs) are endogenous proteins whose function is to maintain the cell's tolerance to insult, and glutamine supplementation is known to increase HSP expression during intense exercise. Since few studies have addressed the possibility that supplementation with other amino acids could have similar effects to that of glutamine, our objective was to evaluate the effects of leucine, valine, isoleucine and arginine as potential stimulators of HSPs 25, 60, 70 and 90 in rats subjected to acute exercise as a stressing factor...
August 16, 2017: Food & Function
https://www.readbyqxmd.com/read/28812232/introgression-of-heat-shock-protein-hsp70-and-shsp-genes-into-the-malaysian-elite-chilli-variety-kulai-capsicum-annuum-l-through-the-application-of-marker-assisted-backcrossing-mab
#13
Magaji G Usman, Mohd Y Rafii, Mohammad Y Martini, Oladosu A Yusuff, Mohd R Ismail, Gous Miah
Backcrossing together with simple sequence repeat marker strategy was adopted to improve popular Malaysian chilli Kulai (Capsicum annuum L.) for heat tolerance. The use of molecular markers in backcross breeding and selection contributes significantly to overcoming the main drawbacks such as increase linkage drag and time consumption, in the ancient manual breeding approach (conventional), and speeds up the genome recovery of the recurrent parent. The strategy was adopted to introgress heat shock protein gene(s) from AVPP0702 (C...
August 15, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28812023/use-of-a-polyethylene-bag-to-reduce-perioperative-regional-and-whole-body-heat-losses-in-low-birth-weight-neonates
#14
Pierre Tourneux, Estelle Durand, Amandine Pelletier, Laurent Ghyselen, Véronique Bach, Jean-Pierre Libert
In the delivery room, wrapping a low-birth-weight neonate (defined as ≤2.499 g) in a polyethylene bag reduces the risk of hypothermia. However, extended use of the bag (e.g., during neonatal surgery) might conceivably increase the risk of thermal stress and thus body overheating. Here, we assessed the efficacy of a polyethylene bag in infants assigned to wrap (W) or nonwrap (NW, control) groups during placement of a percutaneous vena cava catheter by applying a new mathematical model that calculates heat exchanges for covered and uncovered body segments...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28811569/transcriptional-response-to-stress-is-pre-wired-by-promoter-and-enhancer-architecture
#15
Anniina Vihervaara, Dig Bijay Mahat, Michael J Guertin, Tinyi Chu, Charles G Danko, John T Lis, Lea Sistonen
Programs of gene expression are executed by a battery of transcription factors that coordinate divergent transcription from a pair of tightly linked core initiation regions of promoters and enhancers. Here, to investigate how divergent transcription is reprogrammed upon stress, we measured nascent RNA synthesis at nucleotide-resolution, and profiled histone H4 acetylation in human cells. Our results globally show that the release of promoter-proximal paused RNA polymerase into elongation functions as a critical switch at which a gene's response to stress is determined...
August 15, 2017: Nature Communications
https://www.readbyqxmd.com/read/28811489/high-temperature-stress-during-flowering-and-grain-filling-offsets-beneficial-impact-of-elevated-co2-on-assimilate-partitioning-and-sink-strength-in-rice
#16
Ashish K Chaturvedi, Rajeev N Bahuguna, Divya Shah, Madan Pal, S V Krishna Jagadish
Elevated [CO2] (e[CO2]) environments have been predicted to improve rice yields under future climate. However, a concomitant rise in temperature could negate e[CO2] impact on plants, presenting a serious challenge for crop improvement. High temperature (HT) stress tolerant NL-44 and high yielding basmati Pusa 1121 rice cultivars, were exposed to e[CO2] (from panicle initiation to maturity) and a combination of e[CO2] + HT (from heading to maturity) using field based open top chambers. Elevated [CO2] significantly increased photosynthesis, seed-set, panicle weight and grain weight across both cultivars, more prominently with Pusa 1121...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28810996/rhabdomyolysis-after-crawling-military-training
#17
Danit Atias-Varon, Haggai Sherman, Ran Yanovich, Yuval Heled
INTRODUCTION: Rhabdomyolysis is a syndrome characterized by muscle necrosis followed by release of intracellular muscle contents into the circulation. Exertional rhabdomyolysis (ER) occurs in response to nonfamiliar and/or excessive, prolonged, or repetitive exercises, with eccentric characteristics. In military populations, due to the type of intense, all out physical loads, ER is a significant threat, particularly when training under heat stress. However, many other etiologies exist, and clinical presentations vary greatly...
July 2017: Military Medicine
https://www.readbyqxmd.com/read/28810683/evaluating-the-physiological-and-perceptual-responses-of-wearing-a-newly-designed-cooling-vest-for-construction-workers
#18
Yijie Zhao, Wen Yi, Albert P C Chan, Francis K W Wong, Michael C H Yam
Construction workers are subjected to heat stress because of the hot environment, physically demanding tasks, and/or personal protective equipment. A tailor-made cooling vest that protects construction workers from heat-related injuries was developed. The purpose of the study is to examine a newly designed cooling vest's effectiveness in alleviating physiological and perceptual strain in a hot and humid environment. Twelve male participants performed two trials, i.e., cooling vest (VEST) and control (CON) in a climatic chamber controlled at 37°C temperature, 60% relative humidity, 0...
August 1, 2017: Annals of Work Exposures and Health
https://www.readbyqxmd.com/read/28810517/argan-oil-reduces-oxidative-stress-genetic-damage-and-emperipolesis-in-rats-treated-with-acrylamide
#19
Zülal Atlı Şekeroğlu, Birsen Aydın, Vedat Şekeroğlu
Acrylamide (AA), a well-known toxicant, is present in high-temperature-processed foods in heated foods. Argan oil (AO), a natural vegetable oil, is receiving increasing attention due to its powerful biological properties. However, limited information is available about its effects in lymphoid organs and bone marrow. The aim of this study is to investigate the effects of AO on hematological parameters, 8-hydroxydeoxyguanosine (8-OHdG), thiobarbituric acid reactive substances (TBARs), protein carbonyl (PCO), glutathione (GSH), myeloperoxidase (MPO) levels, the formation of micronucleus (MN) and megakaryocytic emperipolesis (ME) against AA-induced toxicity in rats...
August 10, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28808654/genome-wide-identification-and-transcriptional-expression-analysis-of-cucumber-superoxide-dismutase-sod-family-in-response-to-various-abiotic-stresses
#20
Yong Zhou, Lifang Hu, Hao Wu, Lunwei Jiang, Shiqiang Liu
Superoxide dismutase (SOD) proteins are widely present in the plant kingdom and play important roles in different biological processes. However, little is known about the SOD genes in cucumber. In this study, night SOD genes were identified from cucumber (Cucumis sativus) using bioinformatics-based methods, including 5 Cu/ZnSODs, 3 FeSODs, and 1 MnSOD. Gene structure and motif analysis indicated that most of the SOD genes have relatively conserved exon/intron arrangement and motif composition. Phylogenetic analyses with SODs from cucumber and several other species revealed that these SOD proteins can be traced back to two ancestral SODs before the divergence of monocot and dicot plants...
2017: International Journal of Genomics
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