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https://www.readbyqxmd.com/read/28088182/overexpression-of-wheat-ferritin-gene-tafer-5b-enhances-tolerance-to-heat-stress-and-other-abiotic-stresses-associated-with-the-ros-scavenging
#1
Xinshan Zang, Xiaoli Geng, Fei Wang, Zhenshan Liu, Liyuan Zhang, Yue Zhao, Xuejun Tian, Zhongfu Ni, Yingyin Yao, Mingming Xin, Zhaorong Hu, Qixin Sun, Huiru Peng
BACKGROUND: The yield of wheat (Triticum aestivum L.), an important crop, is adversely affected by heat stress in many regions of the world. However, the molecular mechanisms underlying thermotolerance are largely unknown. RESULTS: A novel ferritin gene, TaFER, was identified from our previous heat stress-responsive transcriptome analysis of a heat-tolerant wheat cultivar (TAM107). TaFER was mapped to chromosome 5B and named TaFER-5B. Expression pattern analysis revealed that TaFER-5B was induced by heat, polyethylene glycol (PEG), H2O2 and Fe-ethylenediaminedi(o-hydroxyphenylacetic) acid (Fe-EDDHA)...
January 14, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28086798/genome-wide-association-analysis-of-seedling-traits-in-diverse-sorghum-germplasm-under-thermal-stress
#2
Ratan Chopra, Gloria Burow, John J Burke, Nicholas Gladman, Zhanguo Xin
BACKGROUND: Climate variability due to fluctuation in temperature is a worldwide concern that imperils crop production. The need to understand how the germplasm variation in major crops can be utilized to aid in discovering and developing breeding lines that can withstand and adapt to temperature fluctuations is more necessary than ever. Here, we analyzed the genetic variation associated with responses to thermal stresses in a sorghum association panel (SAP) representing major races and working groups to identify single nucleotide polymorphisms (SNPs) that are associated with resilience to temperature stress in a major cereal crop...
January 13, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28081152/increased-serum-sodium-and-serum-osmolarity-are-independent-risk-factors-for-developing-chronic-kidney-disease-5-year-cohort-study
#3
Masanari Kuwabara, Ichiro Hisatome, Carlos A Roncal-Jimenez, Koichiro Niwa, Ana Andres-Hernando, Thomas Jensen, Petter Bjornstad, Tamara Milagres, Christina Cicerchi, Zhilin Song, Gabriela Garcia, Laura G Sánchez-Lozada, Minoru Ohno, Miguel A Lanaspa, Richard J Johnson
BACKGROUND: Epidemics of chronic kidney disease (CKD) not due to diabetes mellitus (DM) or hypertension have been observed among individuals working in hot environments in several areas of the world. Experimental models have documented that recurrent heat stress and water restriction can lead to CKD, and the mechanism may be mediated by hyperosmolarity that activates pathways (vasopressin, aldose reductase-fructokinase) that induce renal injury. Here we tested the hypothesis that elevated serum sodium, which reflects serum osmolality, may be an independent risk factor for the development of CKD...
2017: PloS One
https://www.readbyqxmd.com/read/28077800/oxidative-stress-induced-by-se-deficient-high-energy-diet-implicates-neutrophil-dysfunction-via-nrf2-pathway-suppression-in-swine
#4
Tianshu Yang, Zeping Zhao, Tianqi Liu, Ziwei Zhang, Pengzu Wang, Shiwen Xu, Xin Gen Len, Anshan Shan
The mechanism of the interaction between Se deficiency and high energy remains limited. The aim of the current study was to identify whether Se-deficient, high-energy diet can induce oxidative stress, and downregulate the Nrf2 pathway and phagocytic dysfunction of neutrophils. We detected the phagocytic activity, ROS production, protein levels of Nrf2 and Nrf2 downstream target genes, and the mRNA levels of 25 selenoproteins, heat shock proteins, and cytokines in neutrophils. Cytokine ELISA kits were used to measure the serum cytokines...
January 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28077287/iron-oxide-nanoparticles-modulate-heat-shock-proteins-and-organ-specific-markers-expression-in-mice-male-accessory-organs
#5
Kiruthika Sundarraj, Azhwar Raghunath, Lakshmikanthan Panneerselvam, Ekambaram Perumal
With increased industrial utilization of iron oxide nanoparticles (Fe2O3-NPs), concerns on adverse reproductive health effects following exposure have been immensely raised. In the present study, the effects of Fe2O3-NPs exposure in the seminal vesicle and prostate gland were studied in mice. Mice were exposed to two different doses (25 and 50 mg/kg) of Fe2O3-NPs along with the control and analyzed the expressions of heat shock proteins (HSP60, HSP70 and HSP90) and organ specific markers (Caltrin, PSP94, and SSLP1)...
January 7, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28077209/residual-compressive-surface-stress-increases-the-bending-strength-of-dental-zirconia
#6
Masanao Inokoshi, Fei Zhang, Kim Vanmeensel, Jan De Munck, Shunsuke Minakuchi, Ignace Naert, Jozef Vleugels, Bart Van Meerbeek
OBJECTIVE: To assess the influence of surface treatment and thermal annealing on the four-point bending strength of two ground dental zirconia grades. METHODS: Fully-sintered zirconia specimens (4.0×3.0×45.0mm(3)) of Y-TZP zirconia (LAVA Plus, 3M ESPE) and Y-TZP/Al2O3 zirconia (ZirTough, Kuraray Noritake) were subjected to four surface treatments: (1) 'GROUND': all surfaces were ground with a diamond-coated grinding wheel on a grinding machine; (2) 'GROUND+HEAT': (1) followed by annealing at 1100°C for 30min; (3) 'GROUND+Al2O3 SANDBLASTED': (1) followed by sandblasting using Al2O3; (4) 'GROUND+CoJet SANDBLASTED': (1) followed by tribochemical silica (CoJet) sandblasting...
January 8, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28077200/expression-of-heat-shock-protein-70-in-transport-stressed-broiler-pectoralis-major-muscle-and-its-relationship-with-meat-quality
#7
T Xing, M F Wang, M Y Han, X S Zhu, X L Xu, G H Zhou
Omics research has indicated that heat shock protein 70 (HSP70) is a potential biomarker of meat quality. However, the specific changes and the potential role of HSP70 in postmortem meat quality development need to be further defined. In this study, Arbor Acres broiler chickens (n=126) were randomly categorized into three treatment groups of unstressed control (C), 0.5-h transport (T) and subsequent water shower spray following transport (T/W). Each treatment consisted of six replicates with seven birds each...
January 12, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28077174/mutant-hspb1-causes-loss-of-translational-repression-by-binding-to-pcbp1-an-rna-binding-protein-with-a-possible-role-in-neurodegenerative-disease
#8
Thomas Geuens, Vicky De Winter, Nicholas Rajan, Tilmann Achsel, Ligia Mateiu, Leonardo Almeida-Souza, Bob Asselbergh, Delphine Bouhy, Michaela Auer-Grumbach, Claudia Bagni, Vincent Timmerman
The small heat shock protein HSPB1 (Hsp27) is an ubiquitously expressed molecular chaperone able to regulate various cellular functions like actin dynamics, oxidative stress regulation and anti-apoptosis. So far disease causing mutations in HSPB1 have been associated with neurodegenerative diseases such as distal hereditary motor neuropathy, Charcot-Marie-Tooth disease and amyotrophic lateral sclerosis. Most mutations in HSPB1 target its highly conserved α-crystallin domain, while other mutations affect the C- or N-terminal regions or its promotor...
January 11, 2017: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/28076898/characterization-of-the-hog1-mapk-pathway-in-the-entomopathogenic-fungus-beauveria-bassiana
#9
Jing Liu, Zhi-Kang Wang, Huan-Huan Sun, Sheng-Hua Ying, Ming-Guang Feng
High-osmolarity glycerol (HOG) pathway required for yeast osmoregulation relies upon the mitogen-activated protein kinase (MAPK) Hog1 cascade that comprise the MAPKKKs Ssk2/Ssk22 and Ste11 converging on the MAPKK Pbs2. Here we show a Hog1 cascade with the unique MAPKKK Ssk2 acting in Beauveria bassiana. Hypersensitivity to high osmolarity and high resistance to fludioxonil fungicide appeared in Δssk2, Δpbs2 and Δhog1 mutants whereas the two hallmark phenotypes were reversed in Δste11. Increased sensitivity to heat shock and decreased sensitivity to cell wall perturbation also occurred in the three mutants but not in Δste11 although antioxidant phenotypes were different in all deletion mutants...
January 11, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28076789/reduced-insulin-igf-1-signaling-restores-the-dynamic-properties-of-key-stress-granule-proteins-during-aging
#10
Marie C Lechler, Emily D Crawford, Nicole Groh, Katja Widmaier, Raimund Jung, Janine Kirstein, Jonathan C Trinidad, Alma L Burlingame, Della C David
Low-complexity "prion-like" domains in key RNA-binding proteins (RBPs) mediate the reversible assembly of RNA granules. Individual RBPs harboring these domains have been linked to specific neurodegenerative diseases. Although their aggregation in neurodegeneration has been extensively characterized, it remains unknown how the process of aging disturbs RBP dynamics. We show that a wide variety of RNA granule components, including stress granule proteins, become highly insoluble with age in C. elegans and that reduced insulin/insulin-like growth factor 1 (IGF-1) daf-2 receptor signaling efficiently prevents their aggregation...
January 10, 2017: Cell Reports
https://www.readbyqxmd.com/read/28074336/small-heat-shock-proteins-in-ageing-and-age-related-diseases
#11
REVIEW
Nikolaos Charmpilas, Emmanouil Kyriakakis, Nektarios Tavernarakis
Small heat shock proteins (sHSPs) are gatekeepers of cellular homeostasis across species, preserving proteome integrity under stressful conditions. Nonetheless, recent evidence suggests that sHSPs are more than molecular chaperones with merely auxiliary role. In contrast, sHSPs have emerged as central lifespan determinants, and their malfunction has been associated with the manifestation of neurological disorders, cardiovascular disease and cancer malignancies. In this review, we focus on the role of sHSPs in ageing and age-associated diseases and highlight the most prominent paradigms, where impairment of sHSP function has been implicated in human pathology...
January 10, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28073914/serine-threonine-kinase-unc-51-like-kinase-1-ulk1-phosphorylates-the-co-chaperone-cdc37-and-thereby-disrupts-the-stability-of-cdc37-client-proteins
#12
Ran Li, Fengjie Yuan, Wan Fu, Luyao Zhang, Nan Zhang, Yanan Wang, Ke Ma, Xue Li, Lina Wang, Wei-Guo Zhu, Ying Zhao
The serine/threonine kinase Unc-51 like kinase-1 (Ulk1) is thought to be essential for induction of autophagy, an intracellular bulk degradation process that is activated by various stresses. Although several proteins have been suggested as Ulk1 substrates during autophagic process, it still remains largely unknown about Ulk1's physiological substrates. Here, by performing in vitro and in vivo phosphorylation assay, we report that the co-chaperone cell division cycle protein 37 (Cdc37) is an Ulk1 substrate...
January 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28073535/new-oxylipins-produced-at-the-end-of-a-diatom-bloom-and-their-effects-on-copepod-reproductive-success-and-gene-expression-levels
#13
Chiara Lauritano, Giovanna Romano, Vittoria Roncalli, Angela Amoresano, Carolina Fontanarosa, Mauro Bastianini, Federica Braga, Ylenia Carotenuto, Adrianna Ianora
Diatoms are dominant photosynthetic organisms in the world's oceans and are considered essential in the transfer of energy to higher trophic levels. However, these unicellular organisms produce secondary metabolites deriving from the oxidation of fatty acids, collectively termed oxylipins, with negative effects on predators, such as copepods, that feed on them (e.g. reduction in survival, egg production and hatching success) and, indirectly, on higher trophic levels. Here, a multidisciplinary study (oxylipin measurements, copepod fitness, gene expression analyses, chlorophyll distribution, phytoplankton composition, physico-chemical characteristics) was carried out at the end of the spring diatom bloom in April 2011 in the Northern Adriatic Sea (Mediterranean Sea) in order to deeply investigate copepod-diatom interactions, chemical communication and response pathways...
May 2016: Harmful Algae
https://www.readbyqxmd.com/read/28071840/%C3%AE-adrenergic-blockade-does-not-impair-the-skin-blood-flow-sensitivity-to-local-heating-in-burned-and-non-burned-skin-under-neutral-and-hot-environments-in-children
#14
Eric Rivas, Serina J McEntire, David N Herndon, Ronald P Mlcak, Oscar E Suman
OBJECTIVE: Tested the hypothesis that propranolol, a drug given to burn patients to reduce hypermetabolism/cardiac stress, may inhibit heat dissipation by changing the sensitivity of skin blood flow (SkBF) to local heating under neutral and hot conditions. METHODS: In a randomized double-blind study, a placebo was given to 8 burned children while propranolol was given to 13 burned children with similar characteristics (mean ±SD: 11.9±3y, 147±20cm, 45±23kg, 56±12% TBSA)...
January 10, 2017: Microcirculation: the Official Journal of the Microcirculatory Society, Inc
https://www.readbyqxmd.com/read/28069903/improvement-of-in-vitro-produced-bovine-embryo-treated-with-coa-a-under-heat-stressed-condition
#15
Imran Khan, Kyeong-Lim Lee, Lianguang Xu, Ayman Mesalam, Mmr Chowdhury, Myeong-Don Joo, Ihsan-Ul- Haq, Bushra Mirza, Il-Keun Kong
Heat stress has large effects on reproduction including conception rate in cattle. In this study, we examined the effects of coagulansin-A (coa-A), a steroidal lactone, on acquired thermo tolerance during in vitro production of bovine embryos. Oocytes were incubated in in vitro maturation (IVM) media with or without coa-A at two different temperatures, 40.5 °C and 42 °C, for 20 h. The treatment of coa-A significantly improved blastocyst development only at 40.5 °C (P < 0.05). Interestingly, immunofluorescence analysis demonstrated that coa-A induced heat shock protein 70 (HSP70) and phosphatidylinositol-3-kinase (PI3K), but significantly attenuated nuclear factor kappa B (NF-kB) and cyclooxygenase-2 (COX2)...
January 9, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28069433/dual-recognition-activity-of-a-rhamnose-binding-lectin-to-pathogenic-bacteria-and-zooxanthellae-in-stony-coral-pocillopora-damicornis
#16
Zhi Zhou, Xiaopeng Yu, Jia Tang, Yunjie Zhu, Guangmei Chen, Liping Guo, Bo Huang
Rhamnose-binding lectin (RBL) is a type of Ca(2+)-independent lectin with tandem repeat carbohydrate-recognition domain, and is crucial for the innate immunity in many invertebrates. In this study, the cDNA sequence encoding RBL in coral Pocillopora damicornis (PdRBL-1) was cloned. The PdRBL-1 protein shared highest amino acid sequence similarity (55%) with the polyp of Hydra vulgaris, and contained a signal peptide and two tandem carbohydrate-recognition domains in which all cysteine residues were conserved...
January 6, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28069351/evaluation-of-automated-wes-system-as-an-analytical-and-characterization-tool-to-support-monoclonal-antibody-drug-product-development
#17
Jinyu Wang, Anulfo Valdez, Yingchen Chen
Monitoring and evaluation of critical quality attributes (cQA) in monoclonal antibodies (mAb) are a regulatory requirement in pharmaceutical industry. High molecular weight (HMW) species are of critical importance due to the potential risk associated with immunogenicity. HMW species are typically monitored by size exclusion chromatography (SEC). Although low molecular weight (LMW) species are also detected by SEC, low-resolution separation of LMW limits its capability to monitor mAb fragmentation. Recently, we have developed new methods for LMW characterization and evaluation based on the Wes instrument from ProteinSimple...
December 21, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28069245/extended-glaze-firing-on-ceramics-for-hard-machining-crack-healing-residual-stresses-optical-and-microstructural-aspects
#18
Iana L Aurélio, Lucio S Dorneles, Liliana G May
OBJECTIVE: To evaluate the effect of extended and conventional (manufacturer-recommended) glaze firings on crack healing, residual stresses, optical characteristics and crystalline structure of four ceramics for hard machining. METHODS: Rectangular specimens were obtained by sectioning densely sintered feldspathic (FEL), leucite- (LEU), lithium disilicate- (DIS), and zirconia-reinforced lithium silicate-based (ZLS) prefabricated ceramic blocks and divided into groups according to the applied glaze firing (n=5): conventional glaze/manufacturer-recommended (G), extended glaze (EG) and control/no heat treatment (C)...
January 6, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28069204/enhancement-of-thermal-and-mechanical-properties-of-poly-mma-co-ba-cloisite-30b-nanocomposites-by-ultrasound-assisted-in-situ-emulsion-polymerization
#19
Sachin Sharma, Maneesh Kumar Poddar, Vijayanand S Moholkar
This study reports synthesis and characterization of poly(MMA-co-BA)/Cloisite 30B (organo-modified montmorillonite clay) nanocomposites by ultrasound-assisted in-situ emulsion polymerization. Copolymers have been synthesized with MMA:BA monomer ratio of 4:1, and varying clay loading (1-5wt% monomer). The poly(MMA-co-BA)/Cloisite 30B nanocomposites have been characterized for their thermal and mechanical properties. Ultrasonically synthesized nanocomposites have been revealed to possess higher thermal degradation resistance and mechanical strength than the nanocomposites synthesized using conventional techniques...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28068898/integrated-mrna-and-microrna-analysis-identifies-genes-and-small-mirna-molecules-associated-with-transcriptional-and-post-transcriptional-level-responses-to-both-drought-stress-and-re-watering-treatment-in-tobacco
#20
Qiansi Chen, Meng Li, Zhongchun Zhang, Weiwei Tie, Xia Chen, Lifeng Jin, Niu Zhai, Qingxia Zheng, Jianfeng Zhang, Ran Wang, Guoyun Xu, Hui Zhang, Pingping Liu, Huina Zhou
BACKGROUND: Drought stress is one of the most severe problem limited agricultural productivity worldwide. It has been reported that plants response to drought-stress by sophisticated mechanisms at both transcriptional and post-transcriptional levels. However, the precise molecular mechanisms governing the responses of tobacco leaves to drought stress and water status are not well understood. To identify genes and miRNAs involved in drought-stress responses in tobacco, we performed both mRNA and small RNA sequencing on tobacco leaf samples from the following three treatments: untreated-control (CL), drought stress (DL), and re-watering (WL)...
January 10, 2017: BMC Genomics
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