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https://www.readbyqxmd.com/read/28071032/legume-seeds-and-cereal-grains-capacity-to-accumulate-iron-while-sprouting-in-order-to-obtain-food-fortificant
#1
Magdalena Zielińska-Dawidziak, Halina Staniek, Ewelina Król, Dorota Piasecka-Kwiatkowska, Tomasz Twardowski
BACKGROUND: Prepared sprouts, after culturing in a medium with an increased iron concentration, could become a beneficial food iron fortificant. However, the efficient iron accumulation depends on the plants genus, species and/or varieties. The aim of the study was to indicate the seeds or grains which accumulate iron most efficiently during the sprouting process. METHODS: Alfalfa, lentil, lupine and soybean seeds as well as wheat grains were sprouted in abiotic stress conditions induced by the excess of iron(II) in culture media...
July 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28062461/diverse-bacteria-affiliated-with-the-genera-microvirga-phyllobacterium-and-bradyrhizobium-nodulate-lupinus-micranthus-growing-in-soils-of-northern-tunisia
#2
Abdelhakim Msaddak, David Durán, Mokhtar Rejili, Mohamed Mars, Tomás Ruiz-Argüeso, Juan Imperial, José Palacios, Luis Rey
: The genetic diversity of bacterial populations nodulating Lupinus micranthus in five geographical sites from Northern Tunisia was examined. Phylogenetic analyses of fifty isolates based on recA and gyrB gene partial sequences grouped strains in seven clusters, five of which belong to genus Bradyrhizobium (28 isolates), one to Phyllobacterium (2 isolates), and one, remarkably, to Microvirga (20 isolates). The largest Bradyrhizobium cluster (17 isolates) grouped with the B. lupini species and the other five clusters were close to different, recently defined Bradyrhizobium species...
January 6, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28018392/narrow-leafed-lupin-lupinus-angustifolius-%C3%AE-1-and-%C3%AE-6-conglutin-proteins-exhibit-antifungal-activity-protecting-plants-against-necrotrophic-pathogen-induced-damage-from-sclerotinia-sclerotiorum-and-phytophthora-nicotianae
#3
Jose C Jimenez-Lopez, Su Melser, Kathleen DeBoer, Louise F Thatcher, Lars G Kamphuis, Rhonda C Foley, Karam B Singh
Vicilins (7S globulins) are seed storage proteins and constitute the main protein family in legume seeds, particularly in narrow-leafed lupin (Lupinus angustifolius L.; NLL), where seven vicilin genes, called β1- to β7-conglutin have been identified. Vicilins are involved in germination processes supplying amino acids for seedling growth and plant development, as well as in some cases roles in plant defense and protection against pathogens. The roles of NLL β-conglutins in plant defense are unknown. Here the potential role of five NLL β-conglutin family members in protection against necrotrophic fungal pathogens was investigated and it was demonstrated that recombinant purified 6xHis-tagged β1- and β6-conglutin proteins exhibited the strongest in vitro growth inhibitory activity against a range of necrotrophic fungal pathogens compared to β2, β3, and β4 conglutins...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28013171/asparagine-slows-down-the-breakdown-of-storage-lipid-and-degradation-of-autophagic-bodies-in-sugar-starved-embryo-axes-of-germinating-lupin-seeds
#4
Sławomir Borek, Ewelina Paluch-Lubawa, Stanisława Pukacka, Małgorzata Pietrowska-Borek, Lech Ratajczak
The research was conducted on embryo axes of yellow lupin (Lupinus luteus L.), white lupin (Lupinus albus L.) and Andean lupin (Lupinus mutabilis Sweet), which were isolated from imbibed seeds and cultured for 96h in vitro under different conditions of carbon and nitrogen nutrition. Isolated embryo axes were fed with 60mM sucrose or were sugar-starved. The effect of 35mM asparagine (a central amino acid in the metabolism of germinating lupin seeds) and 35mM nitrate (used as an inorganic kind of nitrogen) on growth, storage lipid breakdown and autophagy was investigated...
December 10, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28012244/%C3%AE-arrow-leafed-lupin-lupinus-angustifolius-l-%C3%AE-conglutin-proteins-modulate-the-insulin-signalling-pathway-as-potential-type-2-diabetes-treatment-and-inflammatory-related-disease-amelioration
#5
Elena Lima-Cabello, Victor Alche, Rhonda C Foley, Sofianos Andrikopoulos, Grant Morahan, Karam B Singh, Juan D Alche, Jose C Jimenez-Lopez
SCOPE: We have investigated the potential use of β-conglutin protein isoforms from narrow-leafed lupin (Lupinus angustifolius L.) as a diabetes treatment. METHODS AND RESULTS: We produced purified recombinant β1-, β2-, β3-, β4-, and β6-conglutin proteins and showed that β1, β3 and β6 could bind to insulin. To assess β-conglutin proteins modulatory effect on insulin-activation meditated kinases, whole blood and peripheral blood mononuclear cell (PBMC) cultures from Type 2 diabetes (T2D) and healthy control subjects (C) were incubated with conglutin proteins...
December 24, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27983830/peptides-derived-from-soy-and-lupin-protein-as-dipeptidyl-peptidase-iv-inhibitors-in-vitro-biochemical-screening-and-in-silico-molecular-modeling-study
#6
Carmen Lammi, Chiara Zanoni, Anna Arnoldi, Giulio Vistoli
Dipeptidyl peptidase IV (DPP-IV) is a new molecular target correlated with the development of type 2 diabetes. Literature describes the identification of some inhibitory peptides from the hydrolysis of different food proteins. This article reports a study on six peptides from soybean and lupin proteins, i.e., Soy 1 (IAVPTGVA), Soy 2 (YVVNPDNDEN), Soy 3 (YVVNPDNNEN), Lup 1 (LTFPGSAED), Lup 2 (LILPKHSDAD), and Lup 3 (GQEQSHQDEGVIVR), which were screened for their capacity to inhibit the activity of DPP-IV, using an in vitro bioassay against human recombinant DPP-IV...
December 28, 2016: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27976189/characterization-of-anthelminthic-properties-of-the-lupine-seed-lupinus-spp
#7
O Dubois, G Sallé, H Février, J B Magnin-Robert, N Harzic, C Charvet, C Neveu, C Enguehard-Gueiffer, L Boudesocque-Delaye
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27975437/transcriptome-sequencing-in-narrow-leafed-lupin-lupinus-angustifolius-combining-short-and-long-read-sequencing-platforms
#8
T Yang, I Nagy, T Asp, F Geu-Flores
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27956868/the-effect-of-dry-yeast-fermentation-on-chemical-composition-and-protein-characteristics-of-blue-lupin-seeds
#9
Małgorzata Kasprowicz-Potocka, Paulina Borowczyk, Anita Zaworska, Włodzimierz Nowak, Andrzej Frankiewicz, Piotr Gulewicz
The effect of 24-hour fermentation of lupin seeds by different yeast strains on their chemical composition was determined. After fermentation, the mass fraction of proteins increased and their in vitro digestibility and biological activity significantly improved. The amino acid profile of fermented products was similar to that of raw lupin seeds. The significant reduction in the mass fraction of oligosaccharides and phytate, but not of alkaloids was found. The pH level of fermented products decreased as a consequence of the increase of lactic and propionic acid mass fractions...
September 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27916928/the-effect-of-lupinus-albus-and-calcium-chloride-on-growth-performance-body-composition-plasma-biochemistry-and-meat-quality-of-male-pigs-immunized-against-gonadotrophin-releasing-factor
#10
Karen Moore, Bruce Mullan, Jae Cheol Kim, Frank Dunshea
Two hundred and ninety-four pigs were used to assess the effect of two ingredients (Lupinus albus (albus lupins) or a combination of calcium chloride and sodium tri-polyphosphate (mineral salts)) on growth performance, body composition and objective meat quality of pigs immunized against gonadotrophin releasing factor (immunocastrates) and entire male pigs in the late finishing phase. Pigs fed mineral salts ate less feed than those fed the control diet with no effect on growth rate (p > 0.05), backfat (p > 0...
December 1, 2016: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/27873194/lupin-and-other-potentially-cross-reactive-allergens-in-peanut-allergy
#11
REVIEW
Maurizio Mennini, Lamia Dahdah, Oscar Mazzina, Alessandro Fiocchi
PURPOSE OF REVIEW: The presence of IgE cross-reactivity between peanut allergens and allergens from other legumes and tree nuts has been demonstrated, but the identification of the involved individual allergens is still limited. The aim of this review is to describe new allergenic findings, of potential relevance for cross-reactivity among peanut and lupin. RECENT FINDINGS: Seventeen allergens of peanut have been included in the official allergen nomenclature database to date...
November 2016: Current Allergy and Asthma Reports
https://www.readbyqxmd.com/read/27868253/the-effect-of-lupinus-albus-seeds-on-digestibility-performance-and-gastrointestinal-tract-indices-in-pigs
#12
M Kasprowicz-Potocka, A Zaworska, S Kaczmarek, M Hejdysz, R Mikuła, A Rutkowski
The effect of soya bean meal (SBM) substitution in the diet with white lupine (WL) seeds on performances and some parameters of the digestive tract of pigs were studied. The digestibility experiment was performed on 30 pigs using difference method combined with marker method, where one group was offered basal diet and in diet of experimental groups 25% of the basal diet was substituted with SBM or WL meal. The growth experiment was conducted on 48 pigs of approximately 20 kg body weight. The animals from the control group were fed with diet containing SBM, whereas in the experimental groups 50% or 100% of SBM was substituted with WL seeds...
November 21, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27862368/effects-of-heat-treatment-on-protein-feeds-evaluated-in%C3%A2-vitro-by-the-method-of-estimating-utilisable-crude-protein-at-the-duodenum
#13
M Vaga, M Hetta, P Huhtanen
The effect of heat treatment on the protein value of field beans, lupins and peas was studied using an in vitro method. Protein feeds were subjected to heat treatment for 30, 60 and 90 min in forced air oven at 120, 140 and 160 °C and in autoclave at 105, 120 and 135 °C. The heat-treated protein feeds were incubated in buffered rumen fluid together with grass silage and barley in complete isonitrogenous diets. The gas production (GP) was recorded continuously, and ammonia-N (NH3 -N) concentrations were determined during the in vitro incubation at 8, 24 and 48 h and used to determine the utilisable crude protein (uCP) at the duodenum at 16 h of incubation (uCP16 )...
November 11, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27858027/%C3%AE-galactosides-present-in-lupin-flour-affect-several-metabolic-parameters-in-wistar-rats
#14
María I Palacio, Adriana R Weisstaub, Ángela Zuleta, Analía I Etcheverría, Guillermo D Manrique
The aim of this study is to evaluate the influence of α-galactosides present in a lupin diet on metabolic parameters in growing Wistar rats. Lupin (Lupinus albus) is a rich source of non-digestible carbohydrates called α-galactosides whose beneficial health effects have been widely reported. For our investigation, twenty male Wistar rats were fed with two different diets: control (C) and lupin flour (L) for a 60-day period. The fermentation of these α-galactosides was monitored by measuring the following indicators: changes in the dynamic lactobacilli and enterobacteriaceae population, cecal content pH, generation of short chain fatty acids (SCFA), Ca and P absorption, and serum lipid (triglycerides and cholesterol) contents...
December 7, 2016: Food & Function
https://www.readbyqxmd.com/read/27810789/multiple-molecular-targets-in-breast-cancer-therapy-by-betulinic-acid
#15
REVIEW
Runlan Luo, Dengyang Fang, Peng Chu, Huajui Wu, Zhuo Zhang, Zeyao Tang
Breast cancer is the second most common type of cancer in the world, and is by far the most prevalent form of cancer in women. However, the efficacy of current treatments for breast cancer is limited. In addition to the high risk of recurrence, some of these have side effects that significantly reduce the quality of life. Therefore, new avenues of treatment for breast cancer are needed. Betulinic acid (BA), a pipeline anticancer drug, exerts anti-proliferative effects on breast cancer cells is mainly through inhibition of cyclin and topoisomerase expression, leading to cell cycle arrest...
December 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27769166/expansion-of-the-phosphatidylethanolamine-binding-protein-family-in-legumes-a-case-study-of-lupinus-angustifolius-l-flowering-locus-t-homologs-lanftc1-and-lanftc2
#16
Michał Książkiewicz, Sandra Rychel, Matthew N Nelson, Katarzyna Wyrwa, Barbara Naganowska, Bogdan Wolko
BACKGROUND: The Arabidopsis FLOWERING LOCUS T (FT) gene, a member of the phosphatidylethanolamine binding protein (PEBP) family, is a major controller of flowering in response to photoperiod, vernalization and light quality. In legumes, FT evolved into three, functionally diversified clades, FTa, FTb and FTc. A milestone achievement in narrow-leafed lupin (Lupinus angustifolius L.) domestication was the loss of vernalization responsiveness at the Ku locus. Recently, one of two existing L...
October 21, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27766362/ultrasensitive-and-rapid-detection-of-%C3%AE-conglutin-combining-aptamers-and-isothermal-recombinase-polymerase-amplification
#17
Miriam Jauset-Rubio, Jonathan Sabaté Del Río, Teresa Mairal, Markéta Svobodová, Mohammad S El-Shahawi, Abdulaziz S Bashammakh, Abdulrahman O Alyoubi, Ciara K O'Sullivan
Lupin is increasingly being used in a variety of food products due to its nutritional, functional and nutraceutical properties. However, several examples of severe and even fatal food-associated anaphylaxis due to lupin inhalation or ingestion have been reported, resulting in the lupin subunit β-conglutin, being defined as the Lup an 1 allergen by the International Union of Immunological Societies (IUIS) in 2008. Here, we report an innovative method termed aptamer-recombinase polymerase amplification (Apta-RPA) exploiting the affinity and specificity of a DNA aptamer selected against the anaphylactic β-conglutin allergen termed β-conglutin binding aptamer II (β-CBA II), facilitating ultrasensitive detection via isothermal amplification...
January 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27738346/autocrine-interleukin-23-promotes-self-renewal-of-cd133-ovarian-cancer-stem-like-cells
#18
Dan Wang, Tong Xiang, Zhongquan Zhao, Kailong Lin, Pin Yin, Lupin Jiang, Zhiqing Liang, Bo Zhu
Cancer stem cells (CSCs) are a group of cells which possess the ability of self-renewing and unlimited proliferation. And these CSCs are thought to be the cause of metastasis, recurrence and resistance. Recent study has found that pro-inflammatory cytokine and chemotactic factor mediate the self-renewing and differentiation of most of CSCs. Thus we speculate that ovarian cancer stem cells (OCSCs) can also maintain the ability of self-renewing and differentiation by releasing inflammatory factor. This report we discuss the biological characteristics and the specific molecular mechanism mediated by interleukin-23 (IL-23) and its receptor on the self-renewing of OCSCs...
October 12, 2016: Oncotarget
https://www.readbyqxmd.com/read/27706815/influence-of-the-isolation-method-on-the-technofunctional-properties-of-protein-isolates-from-lupinus-angustifolius-l
#19
Isabel S Muranyi, Clemens Otto, Claudia Pickardt, Raffael Osen, Peter Koehler, Ute Schweiggert-Weisz
The technofunctional properties of 2 protein isolates from Lupinus angustifolius L. Vitabor isolated by different procedures were investigated. The lupin protein isolate prepared by aqueous alkaline extraction with subsequent isoelectric precipitation (ILP) showed a significantly higher degree of protein denaturation and lower denaturation temperatures than the one obtained by aqueous salt-induced extraction followed by dilutive precipitation (MLP) as determined by differential scanning calorimetry. Rheological investigations revealed higher firmness and a viscoelastic solid-like behavior of ILP, in contrast to MLP that showed viscoelastic, liquid-like properties...
October 5, 2016: Journal of Food Science
https://www.readbyqxmd.com/read/27688257/cranes-and-crops-investigating-farmer-tolerances-toward-crop-damage-by-threatened-blue-cranes-anthropoides-paradiseus-in-the-western-cape-south-africa
#20
Julia L van Velden, Tanya Smith, Peter G Ryan
The Western Cape population of Blue Cranes (Anthropoides paradiseus) in South Africa is of great importance as the largest population throughout its range. However, Blue Cranes are strongly associated with agricultural lands in the Western Cape, and therefore may come into conflict with farmers who perceive them as damaging to crops. We investigated the viability of this population by exploring farmer attitudes toward crane damage in two regions of the Western Cape, the Swartland and Overberg, using semi-structured interviews...
September 29, 2016: Environmental Management
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