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Caterina Villa, Joana Costa, Cristina Gondar, M Beatriz P P Oliveira, Isabel Mafra
Lupine is widely used as an ingredient in diverse food products, but it is also a source of allergens. This work aimed at proposing a method to detect/quantify lupine as an allergen in processed foods based on a normalised real-time PCR assay targeting the Lup a 4 allergen-encoding gene of Lupinus albus. Sensitivities down to 0.0005%, 0.01% and 0.05% (w/w) of lupine in rice flour, wheat flour and bread, respectively, and 1 pg of L. albus DNA were obtained, with adequate real-time PCR performance parameters using the ΔCt method...
October 1, 2018: Food Chemistry
Pascale Henner, Félix Brédoire, Antoine Tailliez, Frédéric Coppin, Sylvie Pierrisnard, Virginie Camilleri, Catherine Keller
Mechanisms of uranium (U) transfer from soil to plants remain poorly understood. The kinetics of supply of U to the soil solution from solid phases could be a key point to understand its phytoavailability and implications for environmental risk assessment. Root activity, particularly the continuous release of organic acids in the rhizosphere, could have an effect on this supply. We tested the impact of citrate exudation by roots of Lupinus albus, either P-sufficient (P+) or P-deficient (P-), on the phytoavailability of U from a naturally contaminated soil (total content of 413 mg U kg-1 ) using a rhizotest design...
May 8, 2018: Journal of Environmental Radioactivity
Mei Lan Fang, Sarah L Canham, Lupin Battersby, Judith Sixsmith, Mineko Wada, Andrew Sixsmith
Background and Objectives: The digital revolution has resulted in innovative solutions and technologies that can support the well-being, independence, and health of seniors. Yet, the notion of the "digital divide" presents significant inequities in terms of who accesses and benefits from the digital landscape. To better understand the social and structural inequities of the digital divide, a realist synthesis was conducted to inform theoretical understandings of information and communication technologies (ICTs); to understand the practicalities of access and use inequities; to uncover practices that facilitate digital literacy and participation; and to recommend policies to mitigate the digital divide...
May 10, 2018: Gerontologist
A López-López, A H Sánchez-Gómez, A Montaño, A Cortés-Delgado, A Garrido-Fernández
This work studies the influence of cultivar and farming area on the sensory profile of green Spanish-style table olives, using Quantitative Descriptive Analysis. The lexicon developed was subsequently applied to samples of Gordal (G), Manzanilla (M), and Hojiblanca (H) from different origins: Arahal (A), Utrera (U), Alameda (Al), Estepa (E), Casariche (C), Alcalá de Guadaira (AG), Posadas (P), and Almendralejo (Am). The analysis of the data by ANOVA, considering the effect of the sample as fixed and those of the panelists and the sessions as random, showed good repeatability (no significant effect of the session)...
June 2018: Food Research International
Kaijian Ling, Lupin Jiang, Shi Liang, Joseph Kwong, Leiyan Yang, Yudi Li, PingYin, Qingchun Deng, Zhiqing Liang
BACKGROUND: Ovarian cancer stem cells (OCSCs) contribute to the poor prognosis of ovarian cancer. Involvement of the androgen receptor (AR) in the malignant behaviors of other tumors has been reported. However, whether AR associates with Nanog (a stem cell marker) and participates in OCSC functions remain unclear. In this study, we investigated the interaction of Nanog with AR and examined whether this interaction induced stem-like properties in ovarian cancer cells. METHODS: AR and Nanog expression in ovarian tumors was evaluated...
May 2, 2018: Journal of Ovarian Research
Candy M Taylor, Lars G Kamphuis, Weilu Zhang, Gagan Garg, Jens D Berger, Mahsa Mousavi-Derazmahalleh, Philipp E Bayer, David Edwards, Karam B Singh, Wallace A Cowling, Matthew N Nelson
Narrow-leafed lupin (Lupinus angustifolius L.) cultivation was transformed by two dominant vernalisation-insensitive, early flowering time loci known as Ku and Julius (Jul), which allowed expansion into shorter season environments. However, reliance on these loci has limited genetic and phenotypic diversity for environmental adaptation in cultivated lupin. We recently predicted that a 1,423 bp deletion in the cis-regulatory region of LanFTc1, a FLOWERING LOCUS T (FT) homologue, de-repressed expression of LanFTc1 and was the underlying cause of the Ku phenotype...
April 20, 2018: Plant, Cell & Environment
Magdalena Karamać, Hakime H Orak, Ryszard Amarowicz, Adnan Orak, Wojciech Piekoszewski
The aim of this study was to compare the antioxidant capacities and phenolic compound profiles of wild and cultivated Lupinus albus L. seeds. The total phenolic content (TPC), radical scavenging activity, ferric-reducing antioxidant power (FRAP) and antioxidant activity in an β-carotene-linoleic acid emulsion were determined. Liquid chromatography-mass spectrometry was used to identify phenolics. The TPC of lupin seeds ranged from 4.36 to 7.24 mg gallic acid equivalent/g dry matter (d.m.). The dominant phenolics of all genotypes were two p-coumaric acid derivatives (0...
August 30, 2018: Food Chemistry
Martina Koeberl, Michael F Sharp, Rongkai Tian, Saman Buddhadasa, Dean Clarke, James Roberts
Lupine belongs to the genus Lupinus and includes three species commonly consumed by humans. The Lupinus genus is closely related to other legumes, such as peanuts, soya, chickpeas, peas, lentils and beans. However, the consumption of lupine (and related legumes) can cause severe allergenic reactions. Therefore, reliable analytical detection methods are required for the analysis of food samples. In this study three commercially available ELISA test kits were analyzed for the detection capability of three common lupine species, as well as cross-reactivity to related legumes...
August 1, 2018: Food Chemistry
Zdeněk Volek, Daniel Bureš, Linda Uhlířová
This work aimed to extend our current knowledge by obtaining novel information regarding the effect of the dietary inclusion of dehulled white lupine seeds (DWL) on the physical characteristics and sensory qualities of rabbit meat, as well as growth and carcass traits. The DWL diet was compared with a soybean meal-based diet. Feeding the DWL diet to rabbits led to lower Warner-Bratzler shear force values measured in grilled meat samples (Longissimus lumborum muscle) across fibers and this finding was confirmed in the sensory evaluation of texture in terms of higher tenderness and fibrosity of meat samples...
March 20, 2018: Meat Science
Marta Kubiś, Sebastian Andrzej Kaczmarek, Sebastian Nowaczewski, Marek Adamski, Marcin Hejdysz, Andrzej Rutkowski
1. The aim of this study was to investigate the effect of white lupin (Lupinus albus) meal (WLM) addition on the intestinal viscosity, bird performance, and nutrient utilisation of laying hens. 2. The experiment was conducted with 360 laying hens aged 21 weeks fed one of six treatments, including a corn-soybean meal control diet (CON) and five experimental diets containing 60, 120, 180, 240, and 300 g/kg WLM. 3. A linear increase in feed intake (P<0.001) was observed with higher levels of WLM from 0 to 300 g/kg...
March 29, 2018: British Poultry Science
Agnieszka Płażek, Franciszek Dubert, Przemysław Kopeć, Michał Dziurka, Agnieszka Kalandyk, Jakub Pastuszak, Bogdan Wolko
Seed imbibition under cold temperature is dangerous when dry seeds have relatively low water content. The aim of this study was to investigate germination of 20 lines/cultivars of narrow-leaf lupine at 7 °C (cold) and 13 °C (control) under the influence of smoke water and following seed hydropriming for 3 h at 20 °C. The efficacy of individual treatments was examined with regard to seed protection during low-temperature germination. Based on seed germination, vigour at cold was evaluated four days after sowing by means of hypocotyl length, the studied lines/cultivars were divided into three groups with low, high and very high germination rates...
March 26, 2018: International Journal of Molecular Sciences
Jennifer A Wood, Hwei-Ting Tan, Helen M Collins, Kuok Yap, Shi Fang Khor, Wai Li Lim, Xiaohui Xing, Vincent Bulone, Rachel A Burton, Geoffrey B Fincher, Matthew R Tucker
Chickpea (Cicer arietinum L.) is an important nutritionally rich legume crop that is consumed worldwide. Prior to cooking, desi chickpea seeds are most often dehulled and cleaved to release the split cotyledons, referred to as dhal. Compositional variation between desi genotypes has a significant impact on nutritional quality and downstream processing, and this has been investigated mainly in terms of starch and protein content. Studies in pulses such as bean and lupin have also implicated cell wall polysaccharides in cooking time variation, but the underlying relationship between desi chickpea cotyledon composition and cooking performance remains unclear...
March 13, 2018: Plant, Cell & Environment
Pirjo Mattila, Sari Mäkinen, Merja Eurola, Taina Jalava, Juha-Matti Pihlava, Jarkko Hellström, Anne Pihlanto
The goal of this work was to analyze nutritional value of various minimally processed commercial products of plant protein sources such as faba bean (Vicia faba), lupin (Lupinus angustifolius), rapeseed press cake (Brassica rapa/napus subsp. Oleifera), flaxseed (Linum usitatissimum), oil hemp seed (Cannabis sativa), buckwheat (Fagopyrum esculentum), and quinoa (Chenopodium quinoa). Basic composition and various nutritional components like amino acids, sugars, minerals, and dietary fiber were determined. Nearly all the samples studied could be considered as good sources of essential amino acids, minerals and dietary fiber...
March 2, 2018: Plant Foods for Human Nutrition
Cécilia Gallardo, Bárbara Hufnagel, Célia Casset, Carine Alcon, Fanny Garcia, Fanchon Divol, Laurence Marquès, Patrick Doumas, Benjamin Péret
Cluster root (CR) is one of the most spectacular plant developmental adaptations to hostile environment. It can be found in a few species from a dozen botanical families, including white lupin (Lupinus albus) in the Fabaceae family. These amazing structures are produced in phosphate-deprived conditions and are made of hundreds of short roots also known as rootlets. White lupin is the only crop bearing CRs and is considered as the model species for CR studies. However, little information is available on CRs atypical development, including the molecular events that trigger their formation...
March 1, 2018: Physiologia Plantarum
Nazli Namazi, Bagher Larijani, Leila Azadbakht
BACKGROUND: Previous trials have reported beneficial effects of isolated and complex fiber supplements in obesity treatment. However, recommending fiber supplements in obese subjects remains controversial. To the best of our knowledge, no systematic review has updated the data on the effects of fiber supplements on obesity since 2010 and the need exists for a systematic review on this topic. Accordingly, a systematic review was undertaken to summarize the efficacy of different types of fiber supplements as a complementary treatment for weight management...
November 1, 2017: Archives of Iranian Medicine
Karen M Frick, Rhonda C Foley, Lars G Kamphuis, Kadambot H M Siddique, Gagan Garg, Karam B Singh
Quinolizidine alkaloids (QAs) are toxic secondary metabolites that complicate the end use of narrow-leafed lupin (NLL; Lupinus angustifolius L.) grain, as levels sometimes exceed the industry limit for its use as a food and feed source. The genotypic and environmental influences on QA production in NLL are poorly understood. Here, the expression of QA biosynthetic genes was analysed in vegetative and reproductive tissues of bitter (high QA) and sweet (low QA) accessions. It was demonstrated that sweet accessions are characterized by lower QA biosynthetic gene expression exclusively in leaf and stem tissues than bitter NLL, consistent with the hypothesis that QAs are predominantly produced in aerial tissues and transported to seeds, rather than synthesized within the seed itself...
February 23, 2018: Plant, Cell & Environment
Nicolas Carton, Christophe Naudin, Guillaume Piva, Rim Baccar, Guénaëlle Corre-Hellou
Early strategies of crop growth and N acquisition can be critical for determining competitive interactions between weeds and crops. Grain legumes and especially lupins are known to be poor competitors against weeds. Grain legumes are known to have low mineral soil N uptake abilities. However, inter- and intraspecific differences in N uptake ability in relation to below-ground traits have received little attention. Our objectives were (i) to measure differences among lupins for a set of traits associated with early growth and N acquisition; (ii) to examine how this variation compares to differences between lupin and a cereal, triticale, and (iii) to assess if mixing lupin with triticale provides a higher potential than does pure lupin regarding plant biomass and mineral soil N acquisition early in the crop cycle...
February 2018: AoB Plants
Xiaoning Yan, Lei Yang, Gaili Feng, Zhuli Yu, Minjie Xiao, Weibin Cai, Yanmei Xing, Shasha Bai, Junqia Guo, Zhiyu Wang, Tao Wang, Rong Zhang
Betulin (BT), a pentacyclic lupine-type triterpenoid natural product, possesses antitumor activity in various types of cancers. However, its clinical development was discouraged due to its low biological activities and poor solubility. We prepared lup-20(29)-en-3β,28-di-yl-nitrooxy acetate (NBT), a derivative of BT, that was chemically modified at position 3 of ring A and C-28 by introducing a NO-releasing moiety. This study mainly explored the mechanism of NBT in treating breast cancer through the crosstalk between apoptosis and autophagy in mitochondria...
February 14, 2018: Cell Death & Disease
Tim Sewczyk, Marieke Hoog Antink, Michael Maas, Stephen Kroll, Sascha Beutel
Food protein hydrolysates are often produced in unspecific industrial batch processes. The hydrolysates composition underlies process-related fluctuations and therefore the obtained peptide fingerprint and bioactive properties may vary. To overcome this obstacle and enable the production of specific hydrolysates with selected peptides, a ceramic capillary system was developed and characterized for the continuous production of a consistent peptide composition. Therefore, the protease Alcalase was immobilized on the surface of aminosilane modified yttria stabilized zirconia capillaries with a pore size of 1...
February 10, 2018: AMB Express
Félicien Meunier, Mohsen Zarebanadkouki, Mutez A Ahmed, Andrea Carminati, Valentin Couvreur, Mathieu Javaux
Improving or maintaining crop productivity under conditions of long term change of soil water availability and atmosphere demand for water is one the big challenges of this century. It requires a deep understanding of crop water acquisition properties, i.e. root system architecture and root hydraulic properties among other characteristics of the soil-plant-atmosphere continuum. A root pressure probe technique was used to measure the root hydraulic conductances of seven-week old maize and lupine plants grown in sandy soil...
January 26, 2018: Journal of Plant Physiology
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