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https://www.readbyqxmd.com/read/28316286/brassica-aphid-hemiptera-aphididae-populations-are-conditioned-by-climatic-variables-and-parasitism-level-a-study-case-of-tri%C3%A3-ngulo-mineiro-brazil
#1
M V Sampaio, A P Korndörfer, J Pujade-Villar, J E A Hubaide, S E Ferreira, S O Arantes, D M Bortoletto, C M Guimarães, J A Sánchez-Espigares, B Caballero-López
Cosmopolitan pests such as Brevicoryne brassicae, Lipaphis pseudobrassicae, and Myzus persicae (Aphididae) cause significant damage to Brassicaceae crops. Assessment of the important biotic and abiotic factors that regulate these pests is an essential step in the development of effective Integrated Pest Management programs for these aphids. This study evaluated the influence of leaf position, precipitation, temperature, and parasitism on populations of L. pseudobrassicae, M. persicae, and B. brassicae in collard greens fields in the Triângulo Mineiro region (Minas Gerais state), Brazil...
March 20, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28299594/dissecting-pistil-responses-to-incompatible-and-compatible-pollen-in-self-incompatibility-brassica-oleracea-using-comparative-proteomics
#2
Jing Zeng, Qiguo Gao, Songmei Shi, Xiaoping Lian, Richard Converse, Hecui Zhang, Xiaohong Yang, Xuesong Ren, Song Chen, Liquan Zhu
Angiosperms have developed self-incompatibility (SI) systems to reject self-pollen, thereby promoting outcrossing. The Brassicaceae belongs to typical sporophytic system, having a single S-locus controlled SI response, and was chosen as a model system to study SI-related intercellular signal transduction. In this regard, the downstream factor of EXO70A1 was unknown. Here, protein two-dimensional electrophoresis (2-DE) method and coupled with matrix-assisted laser desorption ionization/time of flight of flight mass spectrometry (MALDI-TOF -MS) and peptide mass fingerprinting (PMF) was used to further explore the mechanism of SI responses in Brassica oleracea L...
March 16, 2017: Protein Journal
https://www.readbyqxmd.com/read/28298689/postharvest-application-of-brassica-meal-derived-allyl-isothiocyanate-to-kiwifruit-effect-on-fruit-quality-nutraceutical-parameters-and-physiological-response
#3
Luisa Ugolini, Laura Righetti, Katya Carbone, Roberta Paris, Lorena Malaguti, Alessandra Di Francesco, Laura Micheli, Mariano Paliotta, Marta Mari, Luca Lazzeri
The use of natural compounds to preserve fruit quality and develop high value functional products deserves attention especially in the growing industry of processing and packaging ready-to-eat fresh-cut fruit. In this work, potential mechanisms underlying the effects of postharvest biofumigation with brassica meal-derived allyl-isothiocyanate on the physiological responses and quality of 'Hayward' kiwifruits were studied. Fruits were treated with 0.15 mg L(-1) of allyl-isothiocyanate vapours for 5 h and then stored in controlled atmosphere (2% O2, 4...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28296348/consumption-of-a-diet-rich-in-brassica-vegetables-is-associated-with-a-reduced-abundance-of-sulphate-reducing-bacteria-a-randomised-crossover-study
#4
Lee Kellingray, Henri S Tapp, Shikha Saha, Joanne F Doleman, Arjan Narbad, Richard F Mithen
SCOPE: We examined whether a Brassica-rich diet was associated with an increase in the relative abundance of intestinal lactobacilli and sulphate-reducing bacteria (SRB), or alteration to the composition of the gut microbiota, in healthy adults. METHODS AND RESULTS: A randomised crossover study was performed with ten healthy adults who were fed a high- and a low-Brassica diet for 2-week periods, with a 2-week washout phase separating the diets. The high-Brassica diet consisted of six 84 g portions of broccoli, six 84 g portions of cauliflower, and six 300 g portions of a broccoli and sweet potato soup...
March 10, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28295295/palynological-investigation-of-mummified-human-remains
#5
Karl J Reinhard, Marina Milanello do Amaral, Nicole Wall
Pollen analysis was applied to a mummified homicide victim in Nebraska, U.S.A., to determine the location of death. A control sample showed the normal ambient pollen in the garage crime scene. Ambient windborne types, common in the air of the region, dominated the control. Internal samples were analyzed from the sacrum, intestine, and diaphragm. Microfossils were recovered from the rehydrated intestine lumen. The intestinal sample was dominated by Brassica (broccoli). The sacrum sample was high in dietary types but with a showing of ambient types...
March 10, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28292259/genome-wide-association-mapping-and-identification-of-candidate-genes-for-fatty-acid-composition-in-brassica-napus-l-using-snp-markers
#6
Cunmin Qu, Ledong Jia, Fuyou Fu, Huiyan Zhao, Kun Lu, Lijuan Wei, Xinfu Xu, Ying Liang, Shimeng Li, Rui Wang, Jiana Li
BACKGROUND: B. napus (oilseed) is an important source of edible vegetable oil, and its nutritional and economic value is determined by its fatty acid composition and content. RESULTS: Using the Brassica 60 K SNP array, we performed a genome-wide association study of fatty acid composition in a population of 520 genetically diverse oilseed accessions. Using the PCA + K model in TASSEL 5.2.1, we identified 62 genomic regions that were significantly associated with the composition of seven fatty acids, and five consensus regions that mapped to the A2, A8, A9, C1, and C3 chromosomes, respectively, of the Brassica napus Darmor-bzh genome...
March 14, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28291771/real-time-divergent-evolution-in-plants-driven-by-pollinators
#7
Daniel D L Gervasi, Florian P Schiestl
Pollinator-driven diversification is thought to be a major source of floral variation in plants. Our knowledge of this process is, however, limited to indirect assessments of evolutionary changes. Here, we employ experimental evolution with fast cycling Brassica rapa plants to demonstrate adaptive evolution driven by different pollinators. Our study shows pollinator-driven divergent selection as well as divergent evolution in plant traits. Plants pollinated by bumblebees evolved taller size and more fragrant flowers with increased ultraviolet reflection...
March 14, 2017: Nature Communications
https://www.readbyqxmd.com/read/28291231/targeted-deep-sequencing-of-flowering-regulators-in-brassica-napus-reveals-extensive-copy-number-variation
#8
Sarah Schiessl, Bruno Huettel, Diana Kuehn, Richard Reinhardt, Rod J Snowdon
Gene copy number variation (CNV) is increasingly implicated in control of complex trait networks, particularly in polyploid plants like rapeseed (Brassica napus L.) with an evolutionary history of genome restructuring. Here we performed sequence capture to assay nucleotide variation and CNV in a panel of central flowering time regulatory genes across a species-wide diversity set of 280 B. napus accessions. The genes were chosen based on prior knowledge from Arabidopsis thaliana and related Brassica species...
March 14, 2017: Scientific Data
https://www.readbyqxmd.com/read/28290279/storage-related-changes-of-cell-wall-based-dietary-fiber-components-of-broccoli-brassica-oleracea-var-italica-stems
#9
Judith Schäfer, Luisa Stanojlovic, Bernhard Trierweiler, Mirko Bunzel
Storage related changes in the cell wall composition potentially affect the texture of plant-based foods and the physiological effects of cell wall based dietary fiber components. Therefore, a detailed characterization of cell wall polysaccharides and lignins from broccoli stems was performed. Freshly harvested broccoli and broccoli stored at 20°C and 1°C for different periods of time were analyzed. Effects on dietary fiber contents, polysaccharide composition, and on lignin contents/composition were much more pronounced during storage at 20°C than at 1°C...
March 2017: Food Research International
https://www.readbyqxmd.com/read/28288583/genetics-and-fine-mapping-of-a-purple-leaf-gene-bopr-in-ornamental-kale-brassica-oleracea-l-var-acephala
#10
Xiao-Ping Liu, Bao-Zhen Gao, Feng-Qing Han, Zhi-Yuan Fang, Li-Mei Yang, Mu Zhuang, Hong-Hao Lv, Yu-Mei Liu, Zhan-Sheng Li, Cheng-Cheng Cai, Hai-Long Yu, Zhi-Yuan Li, Yang-Yong Zhang
BACKGROUND: Due to its variegated and colorful leaves, ornamental kale (Brassica oleracea L. var. acephala) has become a popular ornamental plant. In this study, we report the fine mapping and analysis of a candidate purple leaf gene using a backcross population and an F2 population derived from two parental lines: W1827 (with white leaves) and P1835 (with purple leaves). RESULTS: Genetic analysis indicated that the purple leaf trait is controlled by a single dominant gene, which we named BoPr...
March 14, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28288105/reduction-of-antinutritional-glucosinolates-in-brassica-oilseeds-by-mutation-of-genes-encoding-transporters
#11
Hussam Hassan Nour-Eldin, Svend Roesen Madsen, Steven Engelen, Morten Egevang Jørgensen, Carl Erik Olsen, Jonathan Sonne Andersen, David Seynnaeve, Thalia Verhoye, Rudy Fulawka, Peter Denolf, Barbara Ann Halkier
The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea)...
March 13, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28286515/genetic-architecture-of-resistance-to-alternaria-brassicae-in-arabidopsis-thaliana-qtl-mapping-reveals-two-major-resistance-conferring-loci
#12
Sivasubramanian Rajarammohan, Amarendra Kumar, Vibha Gupta, Deepak Pental, Akshay K Pradhan, Jagreet Kaur
Alternaria brassicae, a necrotrophic fungal pathogen, causes Alternaria blight, one of the most important diseases of oleiferous Brassica crops. The current study utilized Arabidopsis as a model to decipher the genetic architecture of defense against A. brassicae. Significant phenotypic variation that was largely genetically determined was observed among Arabidopsis accessions in response to pathogen challenge. Three biparental mapping populations were developed from three resistant accessions viz. CIBC-5, Ei-2, and Cvi-0 and two susceptible accessions - Gre-0 and Zdr-1 (commonly crossed to CIBC-5 and Ei-2)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28283390/functional-characterization-of-brassica-napus-dna-topoisomerase-i%C3%AE-1-and-its-effect-on-flowering-time-when-expressed-in-arabidopsis-thaliana
#13
Chenhao Gao, Shuanghui Qi, Kaige Liu, Dong Li, Changyu Jin, Shaowei Duan, Meng Zhang, Mingxun Chen
Previous studies have shown that DNA topoisomerase Iα (AtTOP1α) has specific developmental functions during growth and development in Arabidopsis thaliana. However, little is known about the roles of DNA topoisomerases in the closely related and commercially important plant, rapeseed (Brassica napus). Here, the full-length BnTOP1α-1 coding sequence was cloned from the A2 subgenome of the Brassica napus inbred line L111. We determine that all BnTOP1α paralogs showed differing patterns of expression in different organs of L111, and that when expressed in tobacco leaves as a fusion protein with green fluorescent protein, BnTOP1α-1 localized to the nucleus...
March 8, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28276455/corrigendum-the-green-peach-aphid-myzus-persicae-perform-better-on-pre-infested-chinese-cabbage-brassica-pekinensis-by-enhancing-host-plant-nutritional-quality
#14
He-He Cao, Hui-Ru Liu, Zhan-Feng Zhang, Tong-Xian Liu
No abstract text is available yet for this article.
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28276138/analysis-of-oilseed-rape-stem-weevils-chemical-control-using-damage-rating-scale
#15
Željko Milovac, Miroslav Zorić, Filip Franeta, Sreten Terzić, Olivera Petrović Obradović, Ana Marjanović Jeromela
BACKGROUND: Rape stem weevil (Ceutorhynchus napi Gyll.) and cabbage stem weevil (C. pallidactylus Marsh.) can cause significant yield losses to oilseed rape (Brassica napus L.), and chemical control is often needed to protect crops from these pests. The efficacy of six insecticides, chlorpyriphos + cypermethrin, bifenthrin, alpha-cypermethrin, pirimiphos-methyl, thiacloprid and tau-fluvalinate, was tested in a four year field trial. Besides the standard efficacy analysis expressed through the number of larvae per stem, a damage rating scale was introduced and modelled using a regression model for ordinal categorical data...
March 8, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28270831/genomic-prediction-of-genotypic-effects-with-epistasis-and-environment-interactions-for-yield-related-traits-of-rapeseed-brassica-napus-l
#16
Xiang Luo, Yi Ding, Linzhong Zhang, Yao Yue, John H Snyder, Chaozhi Ma, Jun Zhu
Oilseed rape (Brassica napus) is an economically important oil crop, yet the genetic architecture of its complex traits remain largely unknown. Here, genome-wide association study was conducted for eight yield-related traits to dissect the genetic architecture of additive, dominance, epistasis, and their environment interaction. Additionally, the optimal genotype combination and the breeding value of superior line, superior hybrid and existing best line in mapping population were predicted for each trait in two environments based on the predicted genotypic effects...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28270829/cadmium-disrupts-the-balance-between-hydrogen-peroxide-and-superoxide-radical-by-regulating-endogenous-hydrogen-sulfide-in-the-root-tip-of-brassica-rapa
#17
Wenjing Lv, Lifei Yang, Cunfa Xu, Zhiqi Shi, Jinsong Shao, Ming Xian, Jian Chen
Cd (cadmium) stress always alters the homeostasis of ROS (reactive oxygen species) including H2O2 (hydrogen sulfide) and [Formula: see text] (superoxide radical), leading to the oxidative injury and growth inhibition in plants. In addition to triggering oxidative injury, ROS has been suggested as important regulators modulating root elongation. However, whether and how Cd stress induces the inhibition of root elongation by differentially regulating endogenous H2O2 and [Formula: see text], rather than by inducing oxidative injury, remains elusive...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28267258/normal-human-immune-cells-are-sensitive-to-telomerase-inhibition-by-brassica-derived-3-3-diindolylmethane-partly-mediated-via-er%C3%AE-%C3%AE-ap1-signalling
#18
Corinna Herz, Hoai Thi Thu Tran, Steffen Landerer, Johanna Gaus, Nina Schlotz, Lia Lehr, Wolfgang R Schäfer, Oliver Treeck, Grace Akinyi Odongo, Ivan Skatchov, Evelyn Lamy
SCOPE: Indole-3-carbinol (I3C) and 3,3'-diindolylmethane (DIM) from Brassica plants are regarded as promising anticancer phytochemicals. The enzyme telomerase is a very attractive target for cancer therapeutics; in normal cells such as lymphocytes, it plays a decisive role for cell maintenance. The effect of I3C and DIM on telomerase in normal human immune cells (PBMC) was studied compared to leukaemia cells (HL-60). Signalling of telomerase regulation via estrogen receptor (ER) was addressed...
March 7, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28265986/manipulation-of-auxin-and-cytokinin-balance-during-the-plasmodiophora-brassicae-arabidopsis-thaliana-interaction
#19
Jutta Ludwig-Müller, Susann Auer, Sabine Jülke, Sabine Marschollek
The symptoms of the clubroot disease on Brassica species caused by the obligate biotrophic protist Plasmodiophora brassicae relies, among other factors, on the modulation of plant hormones. Signaling, transport as well as biosynthesis and metabolism are key features how the levels of auxins and cytokinins are controlled. We here describe (a) how to inoculate the model plant Arabidopsis thaliana with P. brassicae, (b) qualitative and quantitative methods to evaluate disease severity in auxin and cytokinin mutants,...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28265748/identification-of-different-cytoplasms-based-on-newly-developed-mitotype-specific-markers-for-marker-assisted-selection-breeding-in-brassica-napus-l
#20
Shuangping Heng, Fengyi Chen, Chao Wei, Kaining Hu, Zonghui Yang, Jing Wen, Bin Yi, Chaozhi Ma, Jinxing Tu, Ping Si, Tingdong Fu, Jinxiong Shen
Different mitotype-specific markers were developed to distinguish different cytoplasms in Brassica napus L. Mitotype-specific markers have been developed to distinguish different mitotypes in plant. And use of molecular markers to identify different mitotypes in Brassica napus would enhance breeding efficiency. Here, we comparatively analyzed six sequenced mitochondrial genomes in Brassica napus and identified collinear block sequences and mitotype-specific sequences (MSSs) of these mitochondrial genomes. The collinear block sequences between mitochondrial genomes of nap, cam, and pol cytoplasmic male sterility (CMS) lines were higher than those of other lines...
March 6, 2017: Plant Cell Reports
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