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https://www.readbyqxmd.com/read/28534383/in-vitro-anticancer-activity-and-cytotoxicity-screening-of-phytochemical-extracts-from-selected-traditional-chinese-medicinal-plants
#1
Chao Liang, Hui Pan, Haizhou Li, Yang Zhao, Yang Feng
PURPOSE: The purpose of this study was to evaluate the anticancer activity of 10 selected traditional Chinese medicinal plants on 4 cancer cell lines, namely MCF-7 (breast carcinoma), BALL-1 (acute lymphoblastic leukemia), Huh-7 (hepatocellular carcinoma), HeLa (cervical carcinoma) and their cytotoxicity on a normal cell line, namely MCF-10A (non-tumorigenic mammary epithelial line), about which there were few reports previously. METHODS: Six phytochemical extracts, namely petroleum ether extract (PEE), chloroform extract (CE), ethyl acetate extract (EAE), n-butanol extract (BE), the remainder after extraction (RE) and water extract (WE) from each plant were prepared...
March 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28533439/gene-introgression-in-weeds-depends-on-initial-gene-location-in-the-crop-brassica-napus-raphanus-raphanistrum-model
#2
Katarzyna Adamczyk-Chauvat, Sabrina Delaunay, Anne Vannier, Caroline François, Gwenaëlle Thomas, Frédérique Eber, Maryse Lodé, Marie Gilet, Virginie Huteau, Jérôme Morice, Sylvie Nègre, Cyril Falentin, Olivier Coriton, Henri Darmency, Bachar Alrustom, Eric Jenczewski, Mathieu Rousseau-Gueutin, Anne-Marie Chèvre
The effect of gene location within a crop genome on its transfer to a weed genome remains an open question for gene flow assessment. To elucidate this question, we analyzed advanced generations of intergeneric hybrids, derived from an initial pollination of known oilseed rape varieties (Brassica napus, AACC, 2n=38) by a local population of wild radish (Raphanus raphanistrum, RrRr, 2n=18). After five generations of recurrent pollination, 307 G5 plants with a chromosome number similar to wild radish were genotyped using 105 B...
May 22, 2017: Genetics
https://www.readbyqxmd.com/read/28529710/introducing-the-brassica-information-portal-towards-integrating-genotypic-and-phenotypic-brassica-crop-data
#3
Annemarie H Eckes, Tomasz Gubała, Piotr Nowakowski, Tomasz Szymczyszyn, Rachel Wells, Judith A Irwin, Carlos Horro, John M Hancock, Graham King, Sarah C Dyer, Wiktor Jurkowski
The Brassica Information Portal (BIP) is a centralised repository for Brassica phenotypic data. Trait data associated with Brassica research and breeding experiments conducted on Brassica crops, used as vegetables, for livestock fodder and biofuels, is hosted on the site, together with information on the experimental plant materials used, as well as trial design. BIP is an open access and open source project, built on the schema of CropStoreDB, and as such can provide trait data management strategies for any crop data...
2017: F1000Research
https://www.readbyqxmd.com/read/28529536/mid-infrared-spectroscopy-combined-with-chemometrics-to-detect-sclerotinia-stem-rot-on-oilseed-rape-brassica-napus-l-leaves
#4
Chu Zhang, Xuping Feng, Jian Wang, Fei Liu, Yong He, Weijun Zhou
BACKGROUND: Detection of plant diseases in a fast and simple way is crucial for timely disease control. Conventionally, plant diseases are accurately identified by DNA, RNA or serology based methods which are time consuming, complex and expensive. Mid-infrared spectroscopy is a promising technique that simplifies the detection procedure for the disease. Mid-infrared spectroscopy was used to identify the spectral differences between healthy and infected oilseed rape leaves. Two different sample sets from two experiments were used to explore and validate the feasibility of using mid-infrared spectroscopy in detecting Sclerotinia stem rot (SSR) on oilseed rape leaves...
2017: Plant Methods
https://www.readbyqxmd.com/read/28527971/identification-and-comprehensive-evaluation-of-reference-genes-for-rt-qpcr-analysis-of-host-gene-expression-in-brassica-juncea-aphid-interaction-using-microarray-data
#5
Chet Ram, Murali Krishna Koramutla, Ramcharan Bhattacharya
Brassica juncea is a chief oil yielding crop in many parts of the world including India. With advancement of molecular techniques, RT-qPCR based study of gene-expression has become an integral part of experimentations in crop breeding. In RT-qPCR, use of appropriate reference gene(s) is pivotal. The virtue of the reference genes, being constant in expression throughout the experimental treatments, needs to be validated case by case. Appropriate reference gene(s) for normalization of gene-expression data in B...
May 11, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28526964/characterization-of-a-new-isolate-of-pepper-chlorotic-spot-virus-from-yunnan-province-china
#6
Kuanyu Zheng, Tsung-Chi Chen, Shyi-Dong Yeh, Md Siddiqur Rahman, Xiaoxia Su, Kuo Wu, Ting Li, Zhongkai Zhang, Jiahong Dong
A new isolate, 14YV733, of pepper chlorotic spot virus (PCSV) from chili peppers in Yunnan province of China was identified. The typical tospoviral particles of 80-120 nm in diameter were observed by electron microscopy. The virus caused systemic symptoms in several solanaceous plants and the Brassica rapa L. Chinensis group with mechanical inoculation. The sap from infected leaves reacted positively to a rabbit antibody to the N protein of watermelon silver mottle virus (WSMoV) in immunoblotting. The S, M, and L RNAs of PCSV-14YV733 are 3310 nts, 4711 nts, and 8913 nts long, respectively...
May 19, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28524727/e-cadherin-%C3%AE-catenin-complex-a-target-for-anticancer-and-antimetastasis-plants-plant-derived-compounds
#7
Majid Tafrihi, Roohollah Nakhaei Sistani
Plants reputed to have cancer-inhibiting potential and putative active components derived from those plants have emerged as an exciting new field in cancer study. Some of these compounds have cancer-inhibiting potential in different clinical staging levels, especially metastasis. A few of them which stabilize cell-cell adhesions are controversial topics. This review article introduces some effective herbal compounds that target E-cadherin/β-catenin protein complex. In this article, at first, we briefly review the structure and function of E-cadherin and β-catenin proteins, Wnt signaling pathway, and its target genes...
May 19, 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28523619/effect-of-calcium-bentonite-on-zn-and-cu-mobility-and-their-accumulation-in-vegetable-growth-in-soil-amended-with-compost-during-consecutive-planting
#8
Quan Wang, Mukesh Kumar Awasthi, Xiuna Ren, Junchao Zhao, Ronghua Li, Feng Shen, Zengqiang Zhang
In this study, greenhouse pot experiment was carried out to investigate the Cu and Zn mobility and their accumulations in pakchoi (Brassica chinensis L.) and Chinese cabbage (Brassica rapachinensis L.) growth in Ca-bentonite-pig manure co-compost (CBC)-soil system during three consecutive planting. Five composts (0, 2.5, 5, 7.5, and 10% Ca-bentonite-pig manure co-composts) amended soil with 5% (w/w) application rate and compared to control without any additive. The results showed that the addition of CBC increased the biomass and chlorophyll content of pakchoi, and the maximum overall yield was found in 7...
May 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28515735/time-course-transcriptome-analysis-of-compatible-and-incompatible-pollen-stigma-interactions-in-brassica-napus-l
#9
Tong Zhang, Changbin Gao, Yao Yue, Zhiquan Liu, Chaozhi Ma, Guilong Zhou, Yong Yang, Zhiqiang Duan, Bing Li, Jing Wen, Bin Yi, Jinxiong Shen, Jinxing Tu, Tingdong Fu
Brassica species exhibit both compatible and incompatible pollen-stigma interactions, however, the underlying molecular mechanisms remain largely unknown. Here, RNA-seq technology was applied in a comprehensive time-course experiment (2, 5, 10, 20, and 30 min) to explore gene expression during compatible/incompatible pollen-stigma interactions in stigma. Moderate changes of gene expression were observed both in compatible pollination (PC) and incompatible pollination (PI) within 10 min, whereas drastic changes showed up by 30 min, especially in PI...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28512383/effects-of-cover-crops-on-pratylenchus-penetrans-and-the-nematode-community-in-carrot-production
#10
Zane J Grabau, Zin Thu Zar Maung, D Corey Noyes, Dean G Baas, Benjamin P Werling, Daniel C Brainard, Haddish Melakeberhan
Cover cropping is a common practice in U.S. Midwest carrot production for soil conservation, and may affect soil ecology and plant-parasitic nematodes-to which carrots are very susceptible. This study assessed the impact of cover crops-oats (Avena sativa), radish (Raphanus sativus) cv. Defender, rape (Brassica napus) cv. Dwarf Essex, and a mixture of oats and radish-on plant-parasitic nematodes and soil ecology based on the nematode community in Michigan carrot production systems. Research was conducted at two field sites where cover crops were grown in Fall 2014 preceding Summer 2015 carrot production...
March 2017: Journal of Nematology
https://www.readbyqxmd.com/read/28507329/breeding-histories-and-selection-criteria-for-oilseed-rape-in-europe-and-china-identified-by-genome-wide-pedigree-dissection
#11
Xiaohua Wang, Yan Long, Nian Wang, Jun Zou, Guangda Ding, Martin R Broadley, Philip J White, Pan Yuan, Qianwen Zhang, Ziliang Luo, Peifa Liu, Hua Zhao, Ying Zhang, Hongmei Cai, Graham J King, Fangsen Xu, Jinling Meng, Lei Shi
Selection breeding has played a key role in the improvement of seed yield and quality in oilseed rape (Brassica napus L.). We genotyped Tapidor (European), Ningyou7 (Chinese) and their progenitors with the Brassica 60 K Illumina Infinium SNP array and mapped a total of 29,347 SNP markers onto the reference genome of Darmor-bzh. Identity by descent (IBD) refers to a haplotype segment of a chromosome inherited from a shared common ancestor. IBDs identified on the C subgenome were larger than those on the A subgenome within both the Tapidor and Ningyou7 pedigrees...
May 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28505203/cytological-and-morphological-analysis-of-hybrids-between-brassicoraphanus-and-brassica-napus-for-introgression-of-clubroot-resistant-trait-into-brassica-napus-l
#12
Zongxiang Zhan, Chinedu Charles Nwafor, Zhaoke Hou, Jianfang Gong, Bin Zhu, Yingfen Jiang, Yongming Zhou, Jiangsheng Wu, Zhongyun Piao, Yue Tong, Chao Liu, Chunyu Zhang
Interspecific hybridization is a powerful tool for improvement of crop species, it has the potential to broaden the genetic base and create new plant forms for breeding programs. Synthetic allopolyploid is a widely-used model for the study of genetic recombination and fixed heterosis in Brassica. In Brassica napus breeding, identification and introgression of new sources of clubroot resistance trait from wild or related species into it by hybridization is a long-term crop management strategy for clubroot disease...
2017: PloS One
https://www.readbyqxmd.com/read/28505096/attenuation-of-drought-stress-in-brassica-seedlings-with-exogenous-application-of-ca-2-and-h%C3%A2-o%C3%A2
#13
Akram Khan, Yasir Anwar, Md Mahadi Hasan, Aqib Iqbal, Muhammad Ali, Hesham F Alharby, Khalid Rehman Hakeem, Mirza Hasanuzzaman
Drought is one of the most common abiotic stresses, affecting the growth and productivity of crop plants globally, particularly in arid and semi-arid regions. Different strategies are used to mitigate the impact of drought among crop plants. Exogenous application of different substances are known to decrease the effects of various abiotic stresses, including drought stress. The aim of this study was to evaluate the effect of Ca(2+) and H₂O₂ in developing drought stress tolerance in Brassica napus "Bulbul-98" seedlings...
May 13, 2017: Plants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28504909/ethanol-extract-of-brassica-rapa-ssp-pekinensis-suppresses-tumor-necrosis-factor-%C3%AE-induced-inflammatory-response-in-human-umbilical-vein-endothelial-cells
#14
Hee Kyoung Joo, Sunga Choi, Yu Ran Lee, Eun Ok Lee, Myoung Soo Park, Yong Pyo Lim, Jong-Tae Park, Byeong Hwa Jeon
Brassica rapa L. ssp. pekinensis, commonly known as Chinese cabbage, is a cruciferous vegetable traditionally consumed in east Asia. Although its habitual consumption could account for the low incidence of chronic vascular inflammation, the therapeutic and protective potential of phytochemicals derived from Chinese cabbage has been poorly studied. In this study, we identified the phenolic compounds, kaempferol and quercetin, from the ethanol extract of Chinese cabbage (EtCC). We show for the first time that EtCC contains effective phytochemicals that suppress tumor necrosis factor (TNF)-α-induced inflammatory response in human umbilical vein endothelial cells...
May 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28503182/genome-wide-snp-markers-based-on-slaf-seq-uncover-breeding-traces-in-rapeseed-brassica-napus-l
#15
Qinghong Zhou, Can Zhou, Wei Zheng, Annaliese S Mason, Shuying Fan, Caijun Wu, Donghui Fu, Yingjin Huang
Single Nucleotide Polymorphisms (SNPs) are the most abundant and richest form of genomic polymorphism, and hence make highly favorable markers for genetic map construction and genome-wide association studies. In this study, a total of 300 rapeseed accessions (278 representative of Chinese germplasm, plus 22 outgroup accessions of different origins and ecotypes) were collected and sequenced using Specific-Locus Amplified Fragment Sequencing (SLAF-seq) technology, obtaining 660.25M reads with an average sequencing depth of 6...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28500683/induction-of-telomere-mediated-chromosomal-truncation-and-behavior-of-truncated-chromosomes-in-brassica-napus
#16
Xiaohong Yan, Chen Li, Jie Yang, Lijun Wang, Chenghong Jiang, Wenhui Wei
Engineered minichromosomes could be stably inherited and serves as a platform for simultaneously transferring and stably expressing multiple genes. Chromosomal truncation mediated by repeats of telomeric sequence is a promising approach for the generation of minichromosomes. In the present work, direct repetitive sequences of Arabidopsis telomere were used to study telomere-mediated truncation of chromosomes in Brassica napus. Transgenes containing alien Arabidopsis telomere were successfully obtained, and Southern blotting and fluorescence in situ hybridization (FISH) results show that the transgenes successfully resulted in chromosomal truncation in B...
May 13, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28499355/erratum-to-genome-wide-association-mapping-and-identification-of-candidate-genes-for-fatty-acid-composition-in-brassica-napus-l-using-snp-markers
#17
Cunmin Qu, Ledong Jia, Fuyou Fu, Huiyan Zhao, Kun Lu, Lijuan Wei, Xinfu Xu, Ying Liang, Shimeng Li, Rui Wang, Jiana Li
No abstract text is available yet for this article.
May 12, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28498521/does-drought-stress-modify-the-effects-of-plant-growth-promoting-rhizobacteria-on-an-aboveground-chewing-herbivore
#18
Maite Fernández de Bobadilla, Julia Friman, Nurmi Pangesti, Marcel Dicke, Joop J A van Loon, Ana Pineda
Soil microbes have important effects on the interactions of plants with their environment, by promoting plant growth, inducing resistance to pests or by conferring tolerance to abiotic stress. However, their effects are variable and the factors responsible for this variation are mainly unknown. Our aim was to assess how drought stress modifies the effect of the non-pathogenic rhizobacterium Pseudomonas simiae WCS417r on plant growth and resistance against the generalist leaf-chewing caterpillar Mamestra brassicae...
May 12, 2017: Insect Science
https://www.readbyqxmd.com/read/28494582/highly-oxygenated-grayanane-diterpenoids-from-flowers-of-pieris-japonica-and-their-structure-activity-relationship-of-antifeedant-activity-against-pieris-brassicae
#19
Xuanqin Chen, Linghuan Gao, Li Yanping, Hongmei Li, Dan Liu, Xiali Liao, Rongtao Li
Phytochemical investigation on the flowers of poisonous plant Pieris japonica resulted in the isolation of six new highly oxygenated grayanane diterpenoids, neopierisoids G-L (1-6), together with ten known ones (7-16). Their structures were elucidated on the basis of extensive NMR technology and mass analysis. The relative configurations of 1-6 were elucidated by analysis of ROESY spectra and comparison of NMR data with the analogues. The absolute configurations of 1-6 were established by the X-ray diffraction analysis of 1 and comparing CD spectra of 1-6...
May 12, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28494283/preliminary-study-of-phytoremediation-of-brownfield-soil-contaminated-by-pahs
#20
Šárka Petrová, Jan Rezek, Petr Soudek, Tomáš Vaněk
Our project was aimed at improving a brownfield in the city of Kladno, where an old steel producing facility used to be in operation. Ecological risk is mainly caused by the processing of co-products during coal production (tars, oils). Knowledge of toxicology and environmental aspects can help us protect human health and the environment. Primarily, we focused on soil sampling and identification of pollutants. Results showed that organic contamination on the site is very high. Average concentration of total petroleum carbon in the soil was about 13g/kg DW, which is much more than the maximum allowed concentration...
May 7, 2017: Science of the Total Environment
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