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https://www.readbyqxmd.com/read/28737913/uplc-q-exactive-orbitrap-ms-based-lipidomics-approach-to-characterize-lipid-extracts-from-bee-pollen-and-their-in-vitro-anti-inflammatory-properties
#1
Qiangqiang Li, Xinwen Liang, Liang Zhao, Zhongyin Zhang, Xiaofeng Xue, Kai Wang, Liming Wu
Bee pollen (BP) is collected by honeybees from flower pollen mixed with nectar and its secretions, with extensive nutritional and therapeutic properties. Lipids are known to be critical contributors for the therapeutic effects of BP and vary depending on different plant sources; however, lipid profiles of BP are not available. Here, an UPLC-Q-Exactive Orbitrap/MS method was established for comprehensive lipidomics analysis of BP derived from three major nectar plants (Brassica campestris L., Camellia sinensis L...
July 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28736570/genome-wide-identification-and-functional-analysis-of-the-calcineurin-b-like-protein-and-calcineurin-b-like-protein-interacting-protein-kinase-gene-families-in-turnip-brassica-rapa-var-rapa
#2
Xin Yin, Qiuli Wang, Qian Chen, Nan Xiang, Yunqiang Yang, Yongping Yang
The calcineurin B-like protein (CBL)-CBL-interacting protein kinase (CIPK) complex has been identified as a primary component in calcium sensors that perceives various stress signals. Turnip (Brassica rapa var. rapa) has been widely cultivated in the Qinghai-Tibet Plateau for a century as a food crop of worldwide economic significance. These CBL-CIPK complexes have been demonstrated to play crucial roles in plant response to various environmental stresses. However, no report is available on the genome-wide characterization of these two gene families in turnip...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28736567/dynamics-of-calcium-during-in-vitro-microspore-embryogenesis-and-in-vivo-microspore-development-in-brassica-napus-and-solanum-melongena
#3
Alba Rivas-Sendra, Antonio Calabuig-Serna, Jose M Seguí-Simarro
Calcium is widely known to have a role as a signaling molecule in many different processes, including stress response and activation of the embryogenic program. However, there are no direct clues about calcium levels during microspore embryogenesis, an experimental process that combines a developmental switch toward embryogenesis and the simultaneous application of different stressing factors. In this work, we used FluoForte, a calcium-specific fluorescent vital dye, to track by confocal microscopy the changes in levels and subcellular distribution of calcium in living rapeseed (B...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28735362/the-role-of-sulforaphane-in-cancer-chemoprevention-and-health-benefits-a-mini-review
#4
Reza Bayat Mokhtari, Narges Baluch, Tina S Homayouni, Zhenya Morgatskaya, Sushil Kumar, Parandis Kazemi, Herman Yeger
Cancer is a multi-stage process resulting from aberrant signaling pathways driving uncontrolled proliferation of transformed cells. The development and progression of cancer from a premalignant lesion towards a metastatic tumor requires accumulation of mutations in many regulatory genes of the cell. Different chemopreventative approaches have been sought to interfere with initiation and control malignant progression. Here we present research on dietary compounds with evidence of cancer prevention activity that highlights the potential beneficial effect of a diet rich in cruciferous vegetables...
July 23, 2017: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/28732175/genome-wide-identification-functional-prediction-and-evolutionary-analysis-of-r2r3-myb-superfamily-in-brassica-napus
#5
Ali Hajiebrahimi, Hajar Owji, Shiva Hemmati
R2R3-MYBs are important transcription factors (TFs) in planta, involved in development and various stress conditions. Phylogenetic analysis showed the presence of 249 R2R3-MYB TFs in Brassica napus called BnaR2R3-MYBs, clustered into 38 clades. BnaR2R3-MYBs were distributed on 19 chromosomes of B. napus. Sixteen gene clusters were identified. BnaR2R3-MYBs were characterized by motif prediction, gene structure analysis and gene ontology. Evolutionary analysis revealed that BnaR2R3-MYBs are mainly formed as a result of whole genome duplication...
July 21, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28732144/influence-of-high-latitude-light-conditions-on-sensory-quality-and-contents-of-health-and-sensory-related-compounds-in-swede-roots-brassica-napus-l-ssp-rapifera-metzg
#6
Jørgen A B Mølmann, Sidsel Fiskaa Hagen, Gunnar B Bengtsson, Tor J Johansen
BACKGROUND: Vegetable growers in Arctic areas usually must rely on market strategies based on regional origin and product quality. Swede roots (rutabaga) were grown in a phytotron to investigate the effect of high latitude light conditions on sensory quality and some health and sensory-related compounds. Experimental treatments included modifications of 24 h natural day length (69° 39' N), by moving plants at daily intervals to dark chambers with either no light, fluorescent growth light and/or low intensity photoperiod extension...
July 21, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28731514/with-or-without-you-effects-of-the-concurrent-range-expansion-of-an-herbivore-and-its-natural-enemy-on-native-species-interactions
#7
David Carrasco, Gaylord A Desurmont, Diane Laplanche, Magali Proffit, Rieta Gols, Paul G Becher, Mattias C Larsson, Ted C J Turlings, Peter Anderson
Global climatic changes may lead to the arrival of multiple range-expanding species from different trophic levels into new habitats, either simultaneously or in quick succession, potentially causing the introduction of manifold novel interactions into native food webs. Unraveling the complex biotic interactions between native and range-expanding species is critical to understand the impact of climate change on community ecology, but experimental evidence is lacking. In a series of laboratory experiments that simulated direct and indirect species interactions, we investigated the effects of the concurrent arrival of a range-expanding insect herbivore in Europe, Spodoptera littoralis, and its associated parasitoid Microplitis rufiventris, on the native herbivore Mamestra brassicae, and its associated parasitoid M...
July 21, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28731285/plant-infection-by-two-different-viruses-induce-contrasting-changes-of-vectors-fitness-and-behavior
#8
Quentin Chesnais, Aude Couty, Maryline Uzest, Véronique Brault, Arnaud Ameline
Insect-vectored plant viruses can induce changes in plant phenotypes, thus influencing plant-vector interactions in a way that may promote their dispersal according to their mode of transmission (i.e. circulative vs. non-circulative). This indirect vector manipulation requires host-virus-vector co-evolution and would thus be effective solely in very specific plant-virus-vector species associations. Some studies suggest this manipulation may depend on multiple factors relative to various intrinsic characteristics of vectors such as transmission efficiency...
July 21, 2017: Insect Science
https://www.readbyqxmd.com/read/28729874/systematic-analysis-of-hsf-family-genes-in-the-brassica-napus-genome-reveals-novel-responses-to-heat-drought-and-high-co2-stresses
#9
Xiaoyi Zhu, Chunqian Huang, Liang Zhang, Hongfang Liu, Jinhui Yu, Zhiyong Hu, Wei Hua
Drought and heat stress are major causes of lost plant crop yield. In the future, high levels of CO2, in combination of other abiotic stress factors, will become a novel source of stress. Little is known of the mechanisms involved in the acclimation responses of plants to this combination of abiotic stress factors, though it has been demonstrated that heat shock transcription factors (Hsfs) are involved in plant response to various abiotic stresses. In this study, we performed a genome-wide identification and a systematic analysis of genes in the Hsf gene family in Brassica napus...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28729817/ssr-marker-variations-in-brassica-species-provide-insight-into-the-origin-and-evolution-of-brassica-amphidiploids
#10
Ajay Kumar Thakur, Kunwar Harendra Singh, Lal Singh, Joghee Nanjundan, Yasin Jeshima Khan, Dhiraj Singh
BACKGROUND: Oilseed Brassica represents an important group of oilseed crops with a long history of evolution and cultivation. To understand the origin and evolution of Brassica amphidiploids, simple sequence repeat (SSR) markers were used to unravel genetic variations in three diploids and three amphidiploid Brassica species of U's triangle along with Eruca sativa as an outlier. RESULTS: Of 124 Brassica-derived SSR loci assayed, 100% cross-transferability was obtained for B...
2018: Hereditas
https://www.readbyqxmd.com/read/28726228/microwave-irradiation-and-citric-acid-assisted-seed-germination-and-phytoextraction-of-nickel-ni-by-brassica-napus-l-morpho-physiological-and-biochemical-alterations-under-ni-stress
#11
Mujahid Farid, Shafaqat Ali, Muhammad Rizwan, Rashid Saeed, Hafiz Muhammad Tauqeer, Rasham Sallah-Ud-Din, Ahmed Azam, Nighat Raza
The complex bio-geochemistry of soil allows pollutant to persist for a longer period of time which further decreased the fertility and natural composition of land. Nickel, an inorganic pollutant, coming from a wide range of industrial and manufacturing units possesses serious threat to soil degradation and crop productivity around the world. The present study was carried to evaluate the combined role of microwave irradiation (MR) and citric acid (CA) on the phytoextraction potential of Brassica napus L. under Ni stress...
July 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28724956/mapping-resistance-responses-to-sclerotinia-infestation-in-introgression-lines-of-brassica-juncea-carrying-genomic-segments-from-wild-brassicaceae-b-fruticulosa
#12
Kusum Rana, Chhaya Atri, Mehak Gupta, Javed Akhatar, Prabhjodh S Sandhu, Nitin Kumar, Ravinder Jaswal, Martin J Barbetti, Surinder S Banga
Sclerotinia stem rot (Sclerotinia sclerotiorum) is a major disease of Brassica oilseeds. As suitable donors to develop resistant cultivars are not available in crop Brassicas, we introgressed resistance from a wild Brassicaceae species, B. fruticulosa. We produced 206 B. juncea-B. fruticulosa introgression lines (ILs). These were assessed for pollen grain fertility, genome size variations and resistance responses to Sclerotinia following stem inoculations under disease-conducive conditions. Of these, 115 ILs showing normal fertility and genome size were selected for cytogenetic characterization using florescent genomic in situ hybridization (Fl-GISH)...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28718657/bitter-taste-of-brassica-vegetables-the-role-of-genetic-factors-receptors-isothiocyanates-glucosinolates-and-flavor-context
#13
Martyna N Wieczorek, Michał Walczak, Marzena Skrzypczak-Zielińska, Henryk H Jeleń
It is well known that consumption of Brassica vegetables has beneficial effect on human's health. The greatest interest is focused on glucosinolates and their hydrolysis products isothiocyanates, due to their potential as cancer preventing compounds. Brassica vegetables are also rich in flavor compounds belonging to many chemical groups. The main sensory sensation related to these vegetable is their characteristic sharp and bitter taste, and unique aroma. Because of these features this group of vegetables is often rejected by consumers...
July 18, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/28718508/development-of-ifox-hunting-as-a-functional-genomics-tool-and-demonstration-of-its-use-to-identify-early-senescence-related-genes-in-the-polyploid-brassica-napus
#14
Juan Ling, Renjie Li, Chinedu Charles Nwafor, Junluo Cheng, Maoteng Li, Qing Xu, Jian Wu, Lu Gan, Qingyong Yang, Chao Liu, Ming Chen, Yongming Zhou, Edgar B Cahoon, Chunyu Zhang
Functional genomics studies of many polyploid crops, including rapeseed (Brassica napus) are constrained by limited tool sets. Here we report development of a gain-of-function platform, termed "iFOX (inducible Full-length cDNA OvereXpressor gene)-Hunting", for inducible expression of B. napus seed cDNAs in Arabidopsis. A Gateway-compatible plant gene expression vector containing a methoxyfenozide-inducible constitutive promoter for transgene expression was developed. This vector was used for cloning of random cDNAs from developing B...
July 17, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28716054/combined-biotic-stresses-trigger-similar-transcriptomic-responses-but-contrasting-resistance-against-a-chewing-herbivore-in-brassica-nigra
#15
Christelle Bonnet, Steve Lassueur, Camille Ponzio, Rieta Gols, Marcel Dicke, Philippe Reymond
BACKGROUND: In nature, plants are frequently exposed to simultaneous biotic stresses that activate distinct and often antagonistic defense signaling pathways. How plants integrate this information and whether they prioritize one stress over the other is not well understood. RESULTS: We investigated the transcriptome signature of the wild annual crucifer, Brassica nigra, in response to eggs and caterpillars of Pieris brassicae butterflies, Brevicoryne brassicae aphids and the bacterial phytopathogen Xanthomonas campestris pv...
July 17, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28714046/effects-of-cd-and-zn-on-physiological-and-anatomical-properties-of-hydroponically-grown-brassica-napus-plants
#16
Martina Benáková, Hassan Ahmadi, Zuzana Dučaiová, Edita Tylová, Stephan Clemens, Jiří Tůma
Clarifying the connection between metal exposure and anatomical changes represents an important challenge for a better understanding of plant phytoextraction potential. A hydroponic screening experiment was carried out to evaluate the effects of combined interactions of Cd and Zn on mineral uptake (Mg, K, Ca, Na) and on the physiological and anatomical characteristics of Brassica napus L cv. Cadeli, Viking, and Navajo. Plants were exposed to 5 μM Cd (CdCl2), 10 μM Zn (ZnSO4), or both Cd + Zn, for 14 days...
July 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28712501/gene-editing-in-polyploid-crops-wheat-camelina-canola-potato-cotton-peanut-sugar-cane-and-citrus
#17
Donald P Weeks
Polyploid crops make up a significant portion of the major food and fiber crops of the world and include wheat, potato, cotton, apple, peanut, citrus, and brassica oilseeds such as rape, canola, and Camelina. The presence of three sets of chromosomes in triploids, four sets in tetraploids, and six sets in hexaploids present significant challenges to conventional plant breeding and, potentially, to efficient use of rapidly emerging gene and genome-editing systems such as zinc finger nucleases, single-stranded oligonucleotides, TALE effector nucleases, and clustered regularly interspaced short palindromic repeats (CRISPR/Cas9)...
2017: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/28711806/improvement-of-pig-manure-compost-lignocellulose-degradation-organic-matter-humification-and-compost-quality-with-medical-stone
#18
Quan Wang, Mukesh Kumar Awasthi, Junchao Zhao, Xiuna Ren, Ronghua Li, Zhen Wang, Meijing Wang, Zengqiang Zhang
The present study aimed to investigate the effect of different concentrations (0%, 2.5%, 5.0%, 7.5% and 10.0%) of medical stone (MS) on the lignocellulose degradation and organic matter humification during pig manure (PM) composting. The results indicated that the addition of MS drastically promoted the organic carbon and lignin degradation. Compared to the control, the decomposition rate of hemicellulose and cellulose was increased by 9.64-27.08% and 2.11-12.07% in MS added treatments. Meanwhile, MS amendment significantly improved the humification of composting process, and the humic acid contents in MS added treatments were 5...
July 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28710130/dead-or-alive-using-membrane-failure-and-chlorophyll-fluorescence-to-predict-mortality-from-drought
#19
Carmela Rosaria Guadagno, Brent E Ewers, Heather N Speckman, Timothy L Aston, Bridger J Huhn, Stanley B DeVore, Joshua T Ladwig, Rachel N Strawn, Cynthia Weinig
Climate models predict widespread increases in both drought intensity and duration in the next decades. Although water deficiency is one of the greatest environmental pressures to plant survival, limited understanding of how plants respond to extreme drought impedes adequate forecasts of how increasing aridity will impact both forest and crop productivity. Drought induces a suite of physiological responses; however, we lack an accurate mechanistic description of plant response to lethal drought that would improve predictive understanding of the timing of lethal drought with climate change...
July 14, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28706533/inhibition-of-histone-h3k9-methylation-by-bix-01294-promotes-stress-induced-microspore-totipotency-and-enhances-embryogenesis-initiation
#20
Eduardo Berenguer, Ivett Bárány, María-Teresa Solís, Yolanda Pérez-Pérez, María C Risueño, Pilar S Testillano
Microspore embryogenesis is a process of cell reprogramming, totipotency acquisition and embryogenesis initiation, induced in vitro by stress treatments and widely used in plant breeding for rapid production of doubled-haploids, but its regulating mechanisms are still largely unknown. Increasing evidence has revealed epigenetic reprogramming during microspore embryogenesis, through DNA methylation, but less is known about the involvement of histone modifications. In this study, we have analyzed the dynamics and possible role of histone H3K9 methylation, a major repressive modification, as well as the effects on microspore embryogenesis initiation of BIX-01294, an inhibitor of histone methylation, tested for the first time in plants, in Brassica napus and Hordeum vulgare...
2017: Frontiers in Plant Science
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