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https://www.readbyqxmd.com/read/28326728/-effect-of-clock-gene-per1-knockdown-on-clock-gene-networks-in-human-oral-squamous-cell-carcinoma
#1
Zhao Qin, Ao Yiran, Yang Kai, Su Xiaoli, Lü Xiaoqiang
OBJECTIVE: This study investigated the effect of clock gene PER1 on the expression levels of other clock genes in clock gene networks in oral squamous cell carcinoma cells. METHODS: We used RNA interference mediated by short hairpin RNAs (shRNAs) to effectively knock down PER1 in SCC15 human oral squamous cell carcinoma cells. Flow cytometry was used to detect the degree of proliferation and apoptosis of the cells after PER1 knockdown, and quantitative real-time PCR was used to detect the mRNA expression levels of the clock genes CLOCK, BMAL1, PER1, PER2, PER3, DEC1, DEC2, CRY1, CRY2, TIM, CKIE, RORA, NPAS2, and REV-ERBA...
February 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28324022/postnatal-ontogeny-of-the-circadian-expression-of-the-adrenal-clock-genes-and-corticosterone-rhythm-in-male-rats
#2
Silvia Liliana Ruiz Roa, Edson Zangiacomi Martinez, Clarissa Silva Martins, Sonir Rauber Antonini, Margaret de Castro, Ayrton Custódio Moreira
The postnatal synchronization of the circadian variation of the adrenal clock genes in mammals remains unknown. We evaluated the postnatal ontogeny of daily variation of clock genes (Clock/Bmal1/Per1/Per2/Per3/Cry1/Cry2/Rorα/Rev-Erbα) and steroidogenesis-related genes (Star and Mc2r) in rat adrenals and its relationship with the emergence of plasma corticosterone rhythm using Cosinor analysis. Plasma corticosterone circadian rhythm was detected from postnatal day (P) P1, with morning acrophase, between zeitgeber time (ZT) ZT0 and ZT2...
January 25, 2017: Endocrinology
https://www.readbyqxmd.com/read/28317486/abnormal-clock-gene-expression-and-locomotor-activity-rhythms-in-two-month-old-female-appswe-ps1de9-mice
#3
Olaide Oyegbami, Hilary M Collins, Marie C Pardon, Fran Jp Ebling, David M Heery, Paula M Moran
In addition to cognitive decline, Alzheimer's disease (AD) is also characterized by agitation and disruptions in activity and sleep. These symptoms typically occur in the evening or at night and have been referred to as 'sundowning'. These symptoms are especially difficult for carers and there are no specific drug treatments. There is increasing evidence that these symptoms reflect an underlying pathology of circadian rhythm generation and transmission. We investigated whether a transgenic mouse model relevant to AD (APPswe/PS1dE9) exhibits circadian alterations in locomotor activity and expression of clock genes involved in the regulation of the circadian cycle...
March 17, 2017: Current Alzheimer Research
https://www.readbyqxmd.com/read/28296937/effects-of-circadian-clock-genes-and-health-related-behavior-on-metabolic-syndrome-in-a-taiwanese-population-evidence-from-association-and-interaction-analysis
#4
Eugene Lin, Po-Hsiu Kuo, Yu-Li Liu, Albert C Yang, Chung-Feng Kao, Shih-Jen Tsai
Increased risk of developing metabolic syndrome (MetS) has been associated with the circadian clock genes. In this study, we assessed whether 29 circadian clock-related genes (including ADCYAP1, ARNTL, ARNTL2, BHLHE40, CLOCK, CRY1, CRY2, CSNK1D, CSNK1E, GSK3B, HCRTR2, KLF10, NFIL3, NPAS2, NR1D1, NR1D2, PER1, PER2, PER3, REV1, RORA, RORB, RORC, SENP3, SERPINE1, TIMELESS, TIPIN, VIP, and VIPR2) are associated with MetS and its individual components independently and/or through complex interactions in a Taiwanese population...
2017: PloS One
https://www.readbyqxmd.com/read/28293892/light-regulated-protein-kinases-based-on-the-cry2-cib1-system
#5
Wignand W D Mühlhäuser, Maximilian Hörner, Wilfried Weber, Gerald Radziwill
Optogenetic approaches enable the control of biological processes in a time- and space-resolved manner. These light-based methods are noninvasive and by using light as sole activator minimize side effects in contrast to chemical inducers. Here, we provide a protocol for the targeted control of the activity of protein kinases in mammalian cells based on the photoreceptor cryptochrome 2 (CRY2) of Arabidopsis thaliana and its interaction partner CIB1. Blue light (450 nm)-induced binding of CRY2 to CIB1 allows the recruitment of a chimeric cytosolic protein kinase AKT1 to the plasma membrane accompanied with stimulation of its kinase activity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28276525/regulation-of-molecular-clock-oscillations-and-phagocytic-activity-via-muscarinic-ca-2-signaling-in-human-retinal-pigment-epithelial-cells
#6
Rina Ikarashi, Honami Akechi, Yuzuki Kanda, Alsawaf Ahmad, Kouhei Takeuchi, Eri Morioka, Takashi Sugiyama, Takashi Ebisawa, Masaaki Ikeda, Masayuki Ikeda
Vertebrate eyes are known to contain circadian clocks, however, the intracellular mechanisms regulating the retinal clockwork remain largely unknown. To address this, we generated a cell line (hRPE-YC) from human retinal pigmental epithelium, which stably co-expressed reporters for molecular clock oscillations (Bmal1-luciferase) and intracellular Ca(2+) concentrations (YC3.6). The hRPE-YC cells demonstrated circadian rhythms in Bmal1 transcription. Also, these cells represented circadian rhythms in Ca(2+)-spiking frequencies, which were canceled by dominant-negative Bmal1 transfections...
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28272509/optogenetic-control-of-the-dab1-signaling-pathway
#7
Liang Wang, Jonathan A Cooper
The Reelin-Dab1 signaling pathway regulates development of the mammalian brain, including neuron migrations in various brain regions, as well as learning and memory in adults. Extracellular Reelin binds to cell surface receptors and activates phosphorylation of the intracellular Dab1 protein. Dab1 is required for most effects of Reelin, but Dab1-independent pathways may contribute. Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A...
March 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28254568/extra-hypothalamic-brain-clocks-in-songbirds-photoperiodic-state-dependent-clock-gene-oscillations-in-night-migratory-blackheaded-buntings-emberiza-melanocephala
#8
Devraj Singh, Vinod Kumar
The avian circadian pacemaker system is comprised of independent clocks in the retina, pineal and hypothalamus, as shown by daily and circadian oscillations of core clock genes (Per2, Cry1, Bmal1 and Clock) in several birds including migratory blackheaded buntings (Emberiza melanocephala). This study investigated the extra-hypothalamic brain circadian clocks in blackheaded buntings, and measured Per2, Cry1, Cry2, Bmal1 and Clock mRNA expressions at 4h intervals over 24h beginning 1h after light-on in the left and right telencephalon, optic tectum and cerebellum, the brain regions involved in several physiological and cognitive functions...
February 24, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28224616/critical-cholangiocarcinogenesis-control-by-cryptochrome-clock-genes
#9
Ali Mteyrek, Elisabeth Filipski, Catherine Guettier, Malgorzata Oklejewicz, Gijsbertus T J van der Horst, Alper Okyar, Francis Lévi
A coordinated network of molecular circadian clocks in individual cells generates 24-hour rhythms in liver metabolism and proliferation. Circadian disruption through chronic jet lag or Per2 clock gene mutation was shown to accelerate hepatocarcinoma development in mice. Since divergent effects were reported for clock genes Per and Cry regarding xenobiotic toxicity, we questioned the role of Cry1 and Cry2 in liver carcinogenesis. Male WT and Cry1(-/-) Cry2(-/-) mice (C57Bl/6 background) were chronically exposed to diethylnitrosamine (DEN) at ZT11...
February 22, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28196586/the-blue-light-dependent-phosphorylation-of-the-cce-domain-determines-the-photosensitivity-of-arabidopsis-cry2
#10
Qin Wang, William D Barshop, Mingdi Bian, Ajay A Vashisht, Reqing He, Xuhong Yu, Bin Liu, Paula Nguyen, Xuanming Liu, Xiaoying Zhao, James A Wohlschlegel, Chentao Lin
No abstract text is available yet for this article.
February 13, 2017: Molecular Plant
https://www.readbyqxmd.com/read/28186127/optogenetic-control-of-cellular-forces-and-mechanotransduction
#11
Léo Valon, Ariadna Marín-Llauradó, Thomas Wyatt, Guillaume Charras, Xavier Trepat
Contractile forces are the end effectors of cell migration, division, morphogenesis, wound healing and cancer invasion. Here we report optogenetic tools to upregulate and downregulate such forces with high spatiotemporal accuracy. The technology relies on controlling the subcellular activation of RhoA using the CRY2/CIBN light-gated dimerizer system. We fused the catalytic domain (DHPH domain) of the RhoA activator ARHGEF11 to CRY2-mCherry (optoGEF-RhoA) and engineered its binding partner CIBN to bind either to the plasma membrane or to the mitochondrial membrane...
February 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/28181538/epigenetic-editing-of-ascl1-gene-in-neural-stem-cells-by-optogenetics
#12
Chiao-Ling Lo, Samrat Roy Choudhury, Joseph Irudayaraj, Feng C Zhou
Enzymes involved in epigenetic processes such as methyltransferases or demethylases are becoming highly utilized for their persistent DNA or histone modifying efficacy. Herein, we have developed an optogenetic toolbox fused to the catalytic domain (CD) of DNA-methyltransferase3A (DNMT3A-CD) or Ten-Eleven Dioxygenase-1 (TET1-CD) for loci-specific alteration of the methylation state at the promoter of Ascl1 (Mash1), a candidate proneuron gene. Optogenetical protein pairs, CRY2 linked to DNMT3A-CD or TET1-CD and CIB1 fused to a Transcription Activator-Like Element (TALE) locating an Ascl1 promoter region, were designed for site specific epigenetic editing...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28155916/dual-origins-of-the-intracellular-circadian-calcium-rhythm-in-the-suprachiasmatic-nucleus
#13
Ryosuke Enoki, Daisuke Ono, Shigeru Kuroda, Sato Honma, Ken-Ichi Honma
In mammals, the master circadian clock is located in the suprachiasmatic nucleus (SCN), where most neurons show circadian rhythms of intracellular Ca(2+) levels. However, the origin of these Ca(2+) rhythms remains largely unknown. In this study, we successfully monitored the intracellular circadian Ca(2+) rhythms together with the circadian PER2 and firing rhythms in a single SCN slice ex vivo, which enabled us to explore the origins. The phase relation between the circadian PER2 and Ca(2+) rhythms, but not between the circadian PER2 and firing rhythms, was significantly altered in Cry1/Cry2 double knockout mice, which display a loss of intercellular synchronization in the SCN...
February 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28117839/the-genetic-overlap-between-mood-disorders-and-cardiometabolic-diseases-a-systematic-review-of-genome-wide-and-candidate-gene-studies
#14
REVIEW
A T Amare, K O Schubert, M Klingler-Hoffmann, S Cohen-Woods, B T Baune
Meta-analyses of genome-wide association studies (meta-GWASs) and candidate gene studies have identified genetic variants associated with cardiovascular diseases, metabolic diseases and mood disorders. Although previous efforts were successful for individual disease conditions (single disease), limited information exists on shared genetic risk between these disorders. This article presents a detailed review and analysis of cardiometabolic diseases risk (CMD-R) genes that are also associated with mood disorders...
January 24, 2017: Translational Psychiatry
https://www.readbyqxmd.com/read/28109907/circadian-gene-variants-and-breast-cancer
#15
Edyta Reszka, Monika Przybek, Olav Muurlink, Beata Pepłonska
The endogenous and self-sustained circadian rhythm generated and maintained in suprachiasmatic nucleus and peripheral tissues can coordinate various molecular, biochemical and physiological processes in living organisms resulting in the adaptation to environmental cues, e.g. light. Multifactorial breast cancer etiology also involves circadian gene alterations, especially among individuals exposed to light at night. Indeed, shift work that causes circadian disruption has been classified by the International Agency for Research on Cancer as a probable human carcinogen, group 2A...
April 1, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28096467/mir-21-mediated-radioresistance-occurs-via-promoting-repair-of-dna-double-strand-breaks
#16
Baocheng Hu, Xiang Wang, Shuofeng Hu, Xiaomin Ying, Ping Wang, Xiangming Zhang, Jian Wang, Hongyan Wang, Ya Wang
miR-21, as an oncogene that overexpresses in most human tumors, is involved in radioresistance; however, the mechanism remains unclear. Here, we demonstrate that miR-21-mediated radioresistance occurs through promoting repair of DNA double strand breaks, which includes facilitating both non-homologous end-joining (NHEJ) and homologous recombination repair (HRR). The miR-21-promoted NHEJ occurs through targeting GSK3B (a novel target of miR-21), which affects the CRY2/PP5 pathway and in turn increases DNA-PKcs activity...
February 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28095742/lithium-response-in-bipolar-disorders-and-core-clock-genes-expression
#17
Pierre A Geoffroy, Emmanuel Curis, Cindie Courtin, Jeverson Moreira, Thomas Morvillers, Bruno Etain, Jean-Louis Laplanche, Frank Bellivier, Cynthia Marie-Claire
OBJECTIVES: We examine if the lithium response is associated with changes in core clock genes expressions. METHODS: The effect of a therapeutic concentration of lithium (1mM) on the expression levels of 17 circadian genes, was examined in lymphoblastoid cell lines (LCLs) derived from two well-characterized groups of BD patients, defined as lithium non-responders (NR, n = 20) or excellent responders (ER, n = 16). Quantitative real-time PCR (qRT-PCR) were conducted at 2, 4 and 8 days (d2, d4, d8) with and without lithium exposure...
January 18, 2017: World Journal of Biological Psychiatry
https://www.readbyqxmd.com/read/28017587/knockout-rescue-embryonic-stem-cell-derived-mouse-reveals-circadian-period-control-by-quality-and-quantity-of-cry1
#18
Koji L Ode, Hideki Ukai, Etsuo A Susaki, Ryohei Narumi, Katsuhiko Matsumoto, Junko Hara, Naoshi Koide, Takaya Abe, Masato T Kanemaki, Hiroshi Kiyonari, Hiroki R Ueda
To conduct comprehensive characterization of molecular properties in organisms, we established an efficient method to produce knockout (KO)-rescue mice within a single generation. We applied this method to produce 20 strains of almost completely embryonic stem cell (ESC)-derived mice ("ES mice") rescued with wild-type and mutant Cry1 gene under a Cry1(-/-):Cry2(-/-) background. A series of both phosphorylation-mimetic and non-phosphorylation-mimetic CRY1 mutants revealed that multisite phosphorylation of CRY1 can serve as a cumulative timer in the mammalian circadian clock...
January 5, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28003802/restricted-feeding-schedules-modulate-in-a-different-manner-the-expression-of-clock-genes-in-rat-hypothalamic-nuclei
#19
Leonardo D De Araujo, Silvia L Roa, Ana C Bueno, Fernanda B Coeli-Lacchini, Clarissa S Martins, Ernane T Uchoa, Jose Antunes-Rodrigues, Lucila L Elias, Paula C Elias, Ayrton C Moreira, Margaret De Castro
Food access restriction is associated to changes in gene expression of the circadian clock system. However, there are only a few studies investigating the effects of non-photic synchronizers, such as food entrainment, on the expression of clock genes in the central oscillators. We hypothesized that different feeding restriction patterns could modulate the expression of clock genes in the suprachiasmatic nucleus (SCN) "master" clock and in extra-SCN oscillators such as the paraventricular (PVN) and arcuate (ARC) hypothalamic nuclei...
2016: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/27998756/effects-of-24-epibrassinolide-and-green-light-on-plastid-gene-transcription-and-cytokinin-content-of-barley-leaves
#20
Marina V Efimova, Radomira Vankova, Victor V Kusnetsov, Raisa P Litvinovskaya, Ilya E Zlobin, Petre Dobrev, Nina P Vedenicheva, Alina L Savchuk, Raisa A Karnachuk, Natalia V Kudryakova, Vladimir V Kuznetsov
In order to evaluate whether brassinosteroids (BS) and green light regulate the transcription of plastid genes in a cross-talk with cytokinins (CKs), transcription rates of 12 plastid genes (ndhF, rrn23, rpoB, psaA, psaB, rrn16, psbA, psbD, psbK, rbcL, atpB, and trnE/trnY) as well as the accumulation of transcripts of some photoreceptors (PHYA, CRY2, CRY1A, and CRY1B) and signaling (SERK and CAS) genes were followed in detached etiolated barley leaves exposed to darkness, green or white light ±1μm 24-epibrassinolide (EBL)...
April 2017: Steroids
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