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https://www.readbyqxmd.com/read/29466738/cry2-is-critical-for-circadian-regulation-of-myogenic-differentiation-by-bclaf1-mediated-mrna-stabilization-of-cyclin-d1-and-tmem176b
#1
Matthew Lowe, Jacob Lage, Ellen Paatela, Dane Munson, Reilly Hostager, Ce Yuan, Nobuko Katoku-Kikyo, Mercedes Ruiz-Estevez, Yoko Asakura, James Staats, Mulan Qahar, Michaela Lohman, Atsushi Asakura, Nobuaki Kikyo
Circadian rhythms regulate cell proliferation and differentiation; however, little is known about their roles in myogenic differentiation. Our synchronized differentiation studies demonstrate that myoblast proliferation and subsequent myotube formation by cell fusion occur in circadian manners. We found that one of the core regulators of circadian rhythms, Cry2, but not Cry1, is critical for the circadian patterns of these two critical steps in myogenic differentiation. This is achieved through the specific interaction between Cry2 and Bclaf1, which stabilizes mRNAs encoding cyclin D1, a G1/S phase transition regulator, and Tmem176b, a transmembrane regulator for myogenic cell fusion...
February 20, 2018: Cell Reports
https://www.readbyqxmd.com/read/29463750/light-activated-protein-interaction-with-high-spatial-subcellular-confinement
#2
Lorena Benedetti, Andrew E S Barentine, Mirko Messa, Heather Wheeler, Joerg Bewersdorf, Pietro De Camilli
Methods to acutely manipulate protein interactions at the subcellular level are powerful tools in cell biology. Several blue-light-dependent optical dimerization tools have been developed. In these systems one protein component of the dimer (the bait) is directed to a specific subcellular location, while the other component (the prey) is fused to the protein of interest. Upon illumination, binding of the prey to the bait results in its subcellular redistribution. Here, we compared and quantified the extent of light-dependent dimer occurrence in small, subcellular volumes controlled by three such tools: Cry2/CIB1, iLID, and Magnets...
February 20, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29455641/circadian-pathway-genetic-variation-and-cancer-risk-evidence-from-genome-wide-association-studies
#3
Simone Mocellin, Saveria Tropea, Clara Benna, Carlo Riccardo Rossi
BACKGROUND: Dysfunction of the circadian clock and single polymorphisms of some circadian genes have been linked to cancer susceptibility, although data are scarce and findings inconsistent. We aimed to investigate the association between circadian pathway genetic variation and risk of developing common cancers based on the findings of genome-wide association studies (GWASs). METHODS: Single nucleotide polymorphisms (SNPs) of 17 circadian genes reported by three GWAS meta-analyses dedicated to breast (Discovery, Biology, and Risk of Inherited Variants in Breast Cancer (DRIVE) Consortium; cases, n = 15,748; controls, n = 18,084), prostate (Elucidating Loci Involved in Prostate Cancer Susceptibility (ELLIPSE) Consortium; cases, n = 14,160; controls, n = 12,724) and lung carcinoma (Transdisciplinary Research In Cancer of the Lung (TRICL) Consortium; cases, n = 12,160; controls, n = 16,838) in patients of European ancestry were utilized to perform pathway analysis by means of the adaptive rank truncated product (ARTP) method...
February 19, 2018: BMC Medicine
https://www.readbyqxmd.com/read/29451423/replication-of-type-2-diabetes-associated-variants-in-a-saudi-arabian-population
#4
Ruifang Li-Gao, Salma Majid Wakil, Brian F Meyer, Nduna Dzimiri, Dennis O Mook-Kanamori
Over 120 type 2 diabetes (T2D) loci have been identified from genome-wide association analysis (GWAS), mainly from Caucasian populations. Very limited knowledge is available on the Saudi Arabian population. In this study, 122 previously reported T2D related variants from 84 loci were examined in a Saudi Arabian cohort of 1,578 individuals (659 T2D cases and 919 controls). Eleven SNPs corresponding to 9 independent loci had a p-value <0.05. If a more stringent Bonferroni threshold of p=4.1×10 -4 (=0.05/122) was applied, none of the SNPs would have reached the significance level...
February 16, 2018: Physiological Genomics
https://www.readbyqxmd.com/read/29432611/when-the-whole-is-not-greater-than-the-sum-of-the-parts-a-critical-review-of-laboratory-bioassay-effects-testing-for-insecticidal-protein-interactions
#5
Frederick S Walters, Gerson Graser, Andrea Burns, Alan Raybould
Many studies have been conducted to investigate synergism among insecticidal proteins; however, a consensus on minimal data requirements and interpretation is lacking. While some have concluded that all additive predictive-type models should be abandoned, we advocate that additivity models can remain useful as assessment tools and that an appropriately designed interaction study will never systematically underestimate the existence of synergism, irrespective of which additivity model (or none at all) may be used...
February 8, 2018: Environmental Entomology
https://www.readbyqxmd.com/read/29408621/integrated-analysis-of-gene-expression-signatures-associated-with-colon-cancer-from-three-datasets
#6
Ping Gao, Miao He, Chunling Zhang, Changhui Geng
PURPOSE: The present study aimed to elucidate the pathogenesis of colon cancer and identify genes associated with tumor development. METHODS: Three datasets, two (GSE74602 and GSE44861) from the Gene Expression Omnibus database and RNA-Seq colon cancer data from The Cancer Genome Atlas data portal, were downloaded. These three datasets were grouped using a meta-analysis approach, and differentially expressed genes (DEGs) were identified between colon tumor samples and adjacent normal samples...
February 3, 2018: Gene
https://www.readbyqxmd.com/read/29371954/integrated-hepatic-transcriptional-and-serum-metabolic-studies-on-circulating-nutrient-metabolism-in-diurnal-laying-hens
#7
Wan Dan, Liu Yi-Lin, Li Guan-Ya, Huang Rui-Lin, Zhang Yi-Ming, Long Ci-Min, Ruan Zheng, Li Lan, Wu Xin, Zhou Xi-Hong, Yin Yu-Long
The aim of the study was to see the diurnal variation of nutrients metabolism and their regulation under the management of large-scaled production. The hepatic transcriptional and serum metabolic studies on circulating nutrient metabolism were investigated in diurnal laying hens. Liver and blood were collected from 36 hens that were slaughtered at 3:30, 7:30, 11:30, 15:30, 19:30, and 23:30 (n = 6), respectively. The serum amino acid, fatty acid and glucose levels, as well as the hepatic transcriptome were analyzed...
December 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/29370529/temporal-expression-of-clock-genes-in-central-and-peripheral-tissues-of-spotted-munia-under-varying-light-conditions-evidence-for-circadian-regulation-of-daily-physiology-in-a-non-photoperiodic-circannual-songbird-species
#8
Neha Agarwal, Ila Mishra, Sangeeta Rani, Vinod Kumar
We investigated if the duration and/or frequency of the light period affect 24-h rhythm of circadian clock genes in central and peripheral tissues of a non-photoperiodic songbird, the spotted munia (Lonchura punctulata), in which a circannual rhythm regulates the reproductive cycle. We monitored activity-rest pattern and measured 24-h mRNA oscillation of core clock (Bmal1, Clock, Per2, Cry1 and Cry2) and clock-controlled (E4bp4, Rorα and Rev-erbα) genes in the hypothalamus, retina, liver and gut of spotted munia subjected to an aberrant light-dark (LD) cycle (3...
January 25, 2018: Chronobiology International
https://www.readbyqxmd.com/read/29338682/soybean-mads-box-gene-gmagl1-promotes-flowering-via-the-photoperiod-pathway
#9
Xuanrui Zeng, Hailun Liu, Hongyang Du, Sujing Wang, Wenming Yang, Yingjun Chi, Jiao Wang, Fang Huang, Deyue Yu
BACKGROUND: The MADS-box transcription factors are an ancient family of genes that regulate numerous physiological and biochemical processes in plants and facilitate the development of floral organs. However, the functions of most of these transcription factors in soybean remain unknown. RESULTS: In this work, a MADS-box gene, GmAGL1, was overexpressed in soybean. Phenotypic analysis showed that GmAGL1 overexpression not only resulted in early maturation but also promoted flowering and affected petal development...
January 16, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29261746/piriformospora-indica-promotes-early-flowering-in-arabidopsis-through-regulation-of-the-photoperiod-and-gibberellin-pathways
#10
Rui Pan, Le Xu, Qiao Wei, Chu Wu, Wenlin Tang, Ralf Oelmüller, Wenying Zhang
Flowering in plants is synchronized by both environmental cues and internal regulatory factors. Previous studies have shown that the endophytic fungus Piriformospora indica promotes the growth and early flowering in Coleus forskohlii (a medicinal plant) and Arabidopsis. To further dissect the impact of P. indica on pathways responsible for flowering time in Arabidopsis, we co-cultivated Arabidopsis with P. indica and used RT-qPCR to analyze the main gene regulation networks involved in flowering. Our results revealed that the symbiotic interaction of Arabidopsis with P...
2017: PloS One
https://www.readbyqxmd.com/read/29248583/gmplp1-a-pas-lov-protein-functions-as-a-possible-new-type-of-blue-light-photoreceptor-in-soybean
#11
Yongguang Li, Yanzheng Zhang, Mengting Li, Qiulan Luo, Ali Inayat Mallano, Ya Jing, Yuhang Zhang, Lin Zhao, Wenbin Li
Light is one of the most important environmental factors for the growth and development of plants. To adapt to changes in day length, the photoreception and transmission of the light signals in plants mainly depend on the various light receptor proteins. The PAS/LOV protein (PLP) has a PAS domain in the N-terminal and LOV domain in the C-terminal and has been confirmed as a new type of blue light receptor in Arabidopsis thaliana. However, the role of its counterpart in soybean remains largely unclear. In this study, the expression pattern of the GmPLP1 under different light qualities was determined by real-time RT-PCR analysis using the cultivar 'DongNong 42', a photosensitive soybean cultivar, suggesting that GmPLP1 was affected by the circadian clock and was a dark-induced gene...
March 1, 2018: Gene
https://www.readbyqxmd.com/read/29192250/coupling-optogenetics-and-light-sheet-microscopy-a-method-to-study-wnt-signaling-during-embryogenesis
#12
Prameet Kaur, Timothy E Saunders, Nicholas S Tolwinski
Optogenetics allows precise, fast and reversible intervention in biological processes. Light-sheet microscopy allows observation of the full course of Drosophila embryonic development from egg to larva. Bringing the two approaches together allows unparalleled precision into the temporal regulation of signaling pathways and cellular processes in vivo. To develop this method, we investigated the regulation of canonical Wnt signaling during anterior-posterior patterning of the Drosophila embryonic epidermis. Cryptochrome 2 (CRY2) from Arabidopsis Thaliana was fused to mCherry fluorescent protein and Drosophila β-catenin to form an easy to visualize optogenetic switch...
November 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29188381/the-effect-of-wolbachia-on-diapause-fecundity-and-clock-gene-expression-in-trichogramma-brassicae-hymenoptera-trichogrammatidae
#13
Somayeh Rahimi-Kaldeh, Ahmad Ashouri, Alireza Bandani, Kenji Tomioka
The short day lengths of late summer in moderate regions are used to induce diapause in various insects. Many studies have shown the maternal effect of photoperiod on diapause induction of Trichogramma wasps, but there is no study to show the relationship between photoperiodic regimes and clock genes in these useful biological control agents. Here, we investigated the role of photoperiods on diapause, fecundity, and clock gene expression (clk, cyc, cry2, per, and timeout) in asexual and sexual Trichogramma brassicae as a model insect to find any differences between two strains...
November 2017: Development Genes and Evolution
https://www.readbyqxmd.com/read/29164122/changes-in-expression-of-the-clock-gene-in-obstructive-sleep-apnea-syndrome-patients-are-not-reverted-by-continuous-positive-airway-pressure-treatment
#14
Susana Moreira, Raquel Rodrigues, André B Barros, Nadja Pejanovic, Ana Neves-Costa, Dora Pedroso, Cláudia Pereira, Dina Fernandes, João Valença Rodrigues, Cristina Barbara, Luís Ferreira Moita
Purpose: Metabolic syndrome and cardiovascular disease are strongly associated with obstructive sleep apnea syndrome (OSAS), which causes substantial changes to normal circadian physiological functions, including metabolic pathways. Because core clock genes are known to be modulated by sleep/vigilance cycles, we asked whether the expression level of mRNA coding for clock genes is altered in non-treated OSAS patients and if it can be corrected by standard continuous positive airway pressure (CPAP) treatment...
2017: Frontiers in Medicine
https://www.readbyqxmd.com/read/29151886/optogenetic-regulation-of-artificial-microrna-improves-h2-production-in-green-alga-chlamydomonas-reinhardtii
#15
Yuting Wang, Xinqin Jiang, Changxing Hu, Ting Sun, Zhiyong Zeng, Xiaoqi Cai, Hui Li, Zhangli Hu
Background: Chlamydomonas reinhardtii is an ideal model organism not only for the study of basic metabolic processes in both plants and animals but also the production of biofuels including hydrogen. Transgenic analysis of C. reinhardtii is now well established and very convenient, but inducible exogenous gene expression systems remain under-studied. The most commonly used heat shock-inducible system has serious effects on algal cell growth and is difficult and costly to control in large-scale culture...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29144171/circadian-gene-methylation-in-rotating-shift-nurses-a-cross-sectional-study
#16
Edyta Reszka, Edyta Wieczorek, Monika Przybek, Ewa Jabłońska, Paweł Kałużny, Agnieszka Bukowska-Damska, Shanbeh Zienolddiny, Beata Pepłońska
Investigating the methylation status of the circadian genes may contribute to a better understanding of the shift work-related circadian disruption in individuals exposed to artificial light at night. In the present study, we determined the methylation status of the circadian genes associated with a shift work pattern among nurses and midwives participating in a cross-sectional study in Lodz, Poland. Quantitative methylation polymerase chain reaction assays were used to assess promoter CpG methylation in PER1, PER2, PER3, CRY1, CRY2, BMAL1, CLOCK, and NPAS2 in genomic DNA from whole blood of 347 women having a rotating-shift work schedule and 363 women working days only...
November 16, 2017: Chronobiology International
https://www.readbyqxmd.com/read/29109286/circadian-clock-cryptochrome-proteins-regulate-autoimmunity
#17
Qi Cao, Xuan Zhao, Jingwen Bai, Sigal Gery, Haibo Sun, De-Chen Lin, Qi Chen, Zhengshan Chen, Lauren Mack, Henry Yang, Ruishu Deng, Xianping Shi, Ling-Wa Chong, Han Cho, Jianjun Xie, Quan-Zhen Li, Markus Müschen, Annette R Atkins, Christopher Liddle, Ruth T Yu, Serhan Alkan, Jonathan W Said, Ye Zheng, Michael Downes, Ronald M Evans, H Phillip Koeffler
The circadian system regulates numerous physiological processes including immune responses. Here, we show that mice deficient of the circadian clock genes Cry1 and Cry2 [Cry double knockout (DKO)] develop an autoimmune phenotype including high serum IgG concentrations, serum antinuclear antibodies, and precipitation of IgG, IgM, and complement 3 in glomeruli and massive infiltration of leukocytes into the lungs and kidneys. Flow cytometry of lymphoid organs revealed decreased pre-B cell numbers and a higher percentage of mature recirculating B cells in the bone marrow, as well as increased numbers of B2 B cells in the peritoneal cavity of Cry DKO mice...
November 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29106308/sleep-quality-and-methylation-status-of-core-circadian-rhythm-genes-among-nurses-and-midwives
#18
Agnieszka Bukowska-Damska, Edyta Reszka, Pawel Kaluzny, Edyta Wieczorek, Monika Przybek, Shanbeh Zienolddiny, Beata Peplonska
ABSTARCT Poor sleep quality or sleep restriction is associated with sleepiness and concentration problems. Moreover, chronic sleep restriction may affect metabolism, hormone secretion patterns and inflammatory responses. Limited recent reports suggest a potential link between sleep deprivation and epigenetic effects such as changes in DNA methylation profiles. The aim of the present study was to assess the potential association between poor sleep quality or sleep duration and the levels of 5-methylcytosine in the promoter regions of PER1, PER2, PER3, BMAL1, CLOCK, CRY1 CRY2 and NPAS2 genes, taking into account rotating night work and chronotype as potential confounders or modifiers...
2017: Chronobiology International
https://www.readbyqxmd.com/read/29100427/aberrant-rhythmic-expression-of-cryptochrome2-regulates-the-radiosensitivity-of-rat-gliomas
#19
Wang Fan, Li Caiyan, Zhu Ling, Zhao Jiayun
In this study, we investigated the role of the clock regulatory protein cryptochrome 2 (Cry2) in determining the radiosensitivity of C6 glioma cells in a rat model. We observed that Cry2 mRNA and protein levels showed aberrant rhythmic periodicity of 8 h in glioma tissues, compared to 24 h in normal brain tissue. Cry2 mRNA and protein levels did not respond to irradiation in normal tissues, but both were increased at the ZT4 (low Cry2) and ZT8 (high Cry2) time points in gliomas. Immunohistochemical staining of PCNA and TUNEL assays demonstrated that high Cry2 expression in glioma tissues was associated with increased cell proliferation and decreased apoptosis...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29099767/the-first-cry2ac-type-protein-toxic-to-helicoverpa-armigera-cloning-and-overexpression-of-cry2ac7-gene-from-sbs-bt1-strain-of-bacillus-thuringiensis
#20
Faiza Saleem, Abdul Rauf Shakoori
The Cry (crystal) proteins from Bacillus thuringiensis are known to have toxicity against a variety of insects and have been exploited to control insect pests through transgenic plants and biopesticides. B. thuringiensis SBS BT-1 carrying the cry2 genes was isolated from soil samples in Pakistan. The 2-kb full length cry2Ac gene was cloned, sequenced, and submitted to the EMBL DNA database (Accession No. AM292031). For expression analysis, Escherichia coli DH5α was transformed with the fragment sub-cloned in pET22b expression vector using NdeI and HindIII restriction sites, and later confirmed by restriction endonuclease analysis...
November 3, 2017: Toxins
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