keyword
MENU ▼
Read by QxMD icon Read
search

Extracellular Dermal Matrix

keyword
https://www.readbyqxmd.com/read/29330036/regulation-of-fibrotic-changes-by-the-synergistic-effects-of-cytokines-dimensionality-and-matrix-towards-the-development-of-an-in-vitro-human-dermal-hypertrophic-scar-model
#1
Shikha Chawla, Sourabh Ghosh
Current therapeutic strategies to reduce scarring in full thickness skin defect offer limited success due to poor understanding of scar tissue formation and the underlying signaling pathways. There is an urgent need to develop human cell based in vitro scar tissue models as animal testing is associated with ethical and logistic complications and inter-species variations. Pro-inflammatory cytokines play critical role in regulating scar development through complex interplay and interaction with the ECM and corresponding signaling pathways...
January 9, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29330021/ccn4-wisp1-controls-cutaneous-wound-healing-by-modulating-proliferation-migration-and-ecm-expression-in-dermal-fibroblasts-via-%C3%AE-5%C3%AE-1-and-tnf%C3%AE
#2
Mitsuaki Ono, Asuka Masaki, Azusa Maeda, Tina M Kilts, Emilio S Hara, Taishi Komori, Hai Pham, Takuo Kuboki, Marian F Young
Understanding the mechanisms that control cutaneous wound healing is crucial to successfully manage repair of damaged skin. The goal of the current study was to uncover novel extracellular matrix (ECM) components that control the wound healing process. Full thickness skin defects were created in mice and used to show CCN4 up-regulation during wound-healing as early as 1 day after surgery, suggesting a role in inflammation and subsequent dermal migration and proliferation. To determine how CCN4 could regulate wound healing we used Ccn4-KO mice and showed they had delayed wound closure accompanied by reduced expression of Col1a1 and Fn mRNA...
January 9, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29327777/the-use-of-acellular-dermal-matrices-adm-in-breast-reconstruction-a-review
#3
Christian Eichler, Carolin Schulz, Nadine Vogt, Mathias Warm
The use of acellular dermal matrices (ADM), sometimes referred to as extracellular matrix (ECM), has become an interesting aspect of breast reconstruction. A great deal of literature is available, totaling over 7000 ADM-based reconstructions. Most often, ADMs are used in a skin sparing mastectomy (SSM) scenario, although heterologous breast augmentation with a sub-pectoral fixation may also require an ADM application. Their use has become an attractive, but expensive option. Available data shows head to head comparisons between individual ADMs to be mostly retrospective in nature with only a few prospective trials available...
December 22, 2017: Surgical Technology International
https://www.readbyqxmd.com/read/29313702/a-comparison-of-scaffold-free-and-scaffold-based-reconstructed-human-skin-models-as-alternatives-to-animal-use
#4
Beste Kinikoglu
Tissue engineered full-thickness human skin substitutes have various applications in the clinic and in the laboratory, such as in the treatment of burns or deep skin defects, and as reconstructed human skin models in the safety testing of drugs and cosmetics and in the fundamental study of skin biology and pathology. So far, different approaches have been proposed for the generation of reconstructed skin, each with its own advantages and disadvantages. Here, the classic tissue engineering approach, based on cell-seeded polymeric scaffolds, is compared with the less-studied cell self-assembly approach, where the cells are coaxed to synthesise their own extracellular matrix (ECM)...
December 2017: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/29303417/evaluation-of-detergent-free-and-detergent-based-methods-for-decellularization-of-murine-skin
#5
Ali Farrokhi, Mohammadreza Pakyari, Layla Nabai, Amir Pourghadiri, Ryan Hartwell, Reza B Jalili, Aziz Ghahary
Acute and chronic wounds contribute to increased morbidity and mortality in affected people and impose significant financial burdens on healthcare systems. For these challenging wounds, Acellular Dermal Matrices (ADMs) have been used as a biological wound coverage. Unlike engineered dermal matrices, ADMs are prepared through the removal of cells from skin, while preserving the extracellular matrix structure and function. In this study, our primary objective was to develop a detergent-free method of decellularization of the skin to mitigate chemical stress on matrix molecules...
January 5, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29289444/mineralization-by-mesenchymal-stromal-cells-is-variously-modulated-depending-on-commercial-platelet-lysate-preparations
#6
Federica Boraldi, Jorge S Burns, Angelica Bartolomeo, Massimo Dominici, Daniela Quaglino
BACKGROUND AIMS: Numerous cellular models have been developed to investigate calcification for regenerative medicine applications and for the identification of therapeutic targets in various complications associated with age-related diseases. However, results have often been contradictory due to specific culture conditions, cell type ontogeny and aging status. Human platelet lysate (hPL) has been recently investigated as valuable alternative to fetal bovine serum (FBS) in cell culture and bone regeneration...
December 27, 2017: Cytotherapy
https://www.readbyqxmd.com/read/29274938/therapies-for-genetic-extracellular-matrix-diseases-of-the-skin
#7
REVIEW
Alexander Nyström, Rocco Bernasconi, Olivier Bornert
A specialized, highly developed dermal extracellular matrix (ECM) provides the skin with its unique mechano-resilient properties and is vital for organ function. Accordingly, genetically acquired deficiency of dermal ECM proteins or proteins essential for the post-translational modification and homeostasis of the dermal ECM, results in diseases affecting the skin. Some of these diseases are lethal or lead to severe complications for the affected individuals. At present limited efficient and evidence-based treatment options exist for genetic ECM diseases of the skin...
December 21, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29259711/recent-advances-in-the-treatment-of-skin-involvement-in-systemic-sclerosis
#8
REVIEW
Yoshihide Asano
Skin fibrosis is a devastating clinical condition commonly seen in skin-restricted and systemic disorders. The goal of skin fibrosis treatment is the restoration of abnormally activated dermal fibroblasts producing the excessive amount of extracellular matrix, which is generally a final consequence of the complex disease process including the activation of vascular and immune systems. Among various skin fibrotic conditions, the molecular mechanisms underlying dermal fibroblast activation have been mostly well studied in systemic sclerosis (SSc)...
2017: Inflammation and Regeneration
https://www.readbyqxmd.com/read/29244830/transcriptome-profiling-identifies-regulators-of-pathogenesis-in-collagen-vi-related-muscular-dystrophy
#9
Russell J Butterfield, Diane M Dunn, Ying Hu, Kory Johnson, Carsten G Bönnemann, Robert B Weiss
OBJECTIVES: The collagen VI related muscular dystrophies (COL6-RD), Ullrich congenital muscular dystrophy (UCMD) and Bethlem myopathy (BM) are among the most common congenital muscular dystrophies and are characterized by distal joint laxity and a combination of distal and proximal joint contractures. Inheritance can be dominant negative (DN) or recessive depending on the type and location of the mutation. DN mutations allow incorporation of abnormal chains into secreted tetramers and are the most commonly identified mutation type in COL6-RD...
2017: PloS One
https://www.readbyqxmd.com/read/29240854/upregulation-of-extracellular-matrix-genes-corroborates-clinical-efcacy-of-human-fibroblast-derived-growth-factors-in-skin-rejuvenation
#10
Kuniko Kadoya, Elizabeth T Makino, Lily I Jiang, Michael Bachelor, Robin Chung, Priscilla Tan, Tsing Cheng, Gail K Naughton, Rahul C Mehta
<p>Skin care products may use various active ingredients to support skin rejuvenation including growth factors and other molecules that help to regenerate extracellular matrix (ECM) and promote skin repair. The biological effect of skin care products with a strong anti-aging claim was assessed in gene expression analyses using an in vitro human skin model. Application of products containing human fbroblast-derived growth factors resulted in signifcant upregulation of genes encoding ECM components including collagens and elas-tin...
December 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/29240284/extracellular-matrix-stromal-vascular-fraction-gel-conditioned-medium-accelerates-wound-healing-in-a-murine-model
#11
Chengliang Deng, Yunfan He, Jingwei Feng, Ziqing Dong, Yao Yao, Hsiaopei Mok, Maohui Lin, Lu Feng
Conditioned medium (CM) is a new treatment modality in regenerative medicine and has shown a successful outcome in wound healing. We recently introduced extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel), an adipose-derived stem cell and adipose native extracellular matrix-enriched product for cytotherapy. This study aimed to evaluate the effect of CM from ECM/SVF-gel (Gel-CM) on wound healing compared with the conventional CM from adipose tissue (Adi-CM) and stem cell (SVF-CM). In vitro wound healing effect of three CMs on keratinocytes and fibroblasts was evaluated in terms of proliferation property, migratory property, and extracellular matrix production...
December 14, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29216889/targeting-the-cross-talk-between-urokinase-receptor-and-formyl-peptide-receptor-type-1-to-prevent-invasion-and-trans-endothelial-migration-of-melanoma-cells
#12
Concetta Ragone, Michele Minopoli, Vincenzo Ingangi, Giovanni Botti, Federica Fratangelo, Antonello Pessi, Maria Patrizia Stoppelli, Paolo Antonio Ascierto, Gennaro Ciliberto, Maria Letizia Motti, Maria Vincenza Carriero
BACKGROUND: Accumulating evidence demonstrates that the Urokinase Receptor (uPAR) regulates tumor cell migration through its assembly in composite regulatory units with transmembrane receptors, and uPAR88-92 is the minimal sequence required to induce cell motility through the Formyl Peptide Receptor type 1 (FPR1). Both uPAR and FPR1 are involved in melanoma tumor progression, suggesting that they may be targeted for therapeutic purposes. In this study, the role of the uPAR-FPR1 cross-talk to sustain melanoma cell ability to invade extracellular matrix and cross endothelial barriers is investigated...
December 8, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29212187/autophagy-protein-lc3-regulates-the-fibrosis-of-hypertrophic-scar-by-controlling-bcl-xl-in-dermal-fibroblasts
#13
Jihong Shi, Shan Shi, Bin Wu, Jian Zhang, Yan Li, Xue Wu, Julei Zhang, Kejia Wang, Bin Zhao, Weixia Cai, Xiaozhi Bai, Dahai Hu, Hao Guan
Hypertrophic scar (HS) is a serious skin fibrotic disease characterized by excessive hypercellularity and extracellular matrix (ECM) component deposition. Autophagy is a tightly regulated physiological process essential for cellular maintenance, differentiation, development and homeostasis. However, during the formation of HS, whether and how autophagy is regulated in dermal fibroblasts are still far from elucidated. Here we detected the autophagic capacity in HS and normal skin (NS) counterparts, explored and verified the key regulatory molecules of autophagy in HS-derived fibroblasts (HSFs), and validated the data using rabbit ear scar model...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29209583/what-makes-the-optimal-wound-healing-material-a-review-of-current-science-and-introduction-of-a-synthetic-nanofabricated-wound-care-scaffold
#14
REVIEW
Matthew R MacEwan, Sarah MacEwan, Tamas R Kovacs, Joel Batts
Wound matrix materials are used to improve the regeneration of dermal and epidermal layers in both acute and chronic wounds. Contemporary wound matrices are primarily composed of biologic materials such as processed xenogeneic and allogeneic tissues. Unfortunately, existing biologic wound matrices possess multiple limitations including poor longevity, durability, strength, and enzymatic resistance required for persistent support for new tissue formation. A fully-synthetic, resorbable electrospun material (Restrata Wound Matrix, Acera, St...
October 2, 2017: Curēus
https://www.readbyqxmd.com/read/29209359/wnt-%C3%AE-catenin-signaling-pathway-regulates-specific-lncrnas-that-impact-dermal-fibroblasts-and-skin-fibrosis
#15
Nathaniel K Mullin, Nikhil V Mallipeddi, Emily Hamburg-Shields, Beatriz Ibarra, Ahmad M Khalil, Radhika P Atit
Wnt/β-catenin signaling is required for embryonic dermal fibroblast cell fate, and dysregulation of this pathway is sufficient to promote fibrosis in adult tissue. The downstream modulators of Wnt/β-catenin signaling required for controlling cell fate and dermal fibrosis remain poorly understood. The discovery of regulatory long non-coding RNAs (lncRNAs) and their pivotal roles as key modulators of gene expression downstream of signaling cascades in various contexts prompted us to investigate their roles in Wnt/β-catenin signaling...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/29202574/effect-of-biostimulation-on-oral-fibroblast-a-pilot-study
#16
A Palmieri, A Avantaggiato, F Cura, R Papalia, M Casale, F Bressi, L Scapoli
Bio-stimulation is a technique in aesthetic medicine in which different drugs such as nucleotides, antioxidants and glucosaminoglycans precursors are injected in the dermis to improving the anabolic function of dermal fibroblasts, i.e., protein synthesis, replication and production of extracellular matrix components. It can be achieved with multiple intra-dermal injections, using two protocols: 1) Polydeoxyribonucleotide (PDRN) plus glucosamine sulphate (Gluc); 2) N-acetylcysteine (NAC) and amino acids (Aa) (named Bio- NAC procedure)...
December 4, 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/29201497/fibromodulin-reduces-scar-formation-in-adult-cutaneous-wounds-by-eliciting-a-fetal-like-phenotype
#17
Zhong Zheng, Aaron W James, Chenshuang Li, Wenlu Jiang, Joyce Z Wang, Grace X Chang, Kevin S Lee, Feng Chen, Emily A Berthiaume, Yao Chen, Hsin Chuan Pan, Eric C Chen, Weiming Li, Zhihe Zhao, Xinli Zhang, Kang Ting, Chia Soo
Blocking transforming growth factor (TGF)β1 signal transduction has been a central strategy for scar reduction; however, this approach appears to be minimally effective. Here, we show that fibromodulin (FMOD), a 59-kD small leucine-rich proteoglycan critical for normal collagen fibrillogenesis, significantly reduces scar formation while simultaneously increasing scar strength in both adult rodent models and porcine wounds, which simulate human cutaneous scar repair. Mechanistically, FMOD uncouples pro-migration/contraction cellular signals from pro-fibrotic signaling by selectively enhancing SMAD3-mediated signal transduction, while reducing AP-1-mediated TGFβ1 auto-induction and fibrotic extracellular matrix accumulation...
2017: Signal Transduction and Targeted Therapy
https://www.readbyqxmd.com/read/29197438/clinical-outcomes-of-comparing-soft-tissue-alternatives-to-free-gingival-graft-a-systematic-review-and-meta-analysis%C3%A2
#18
Irina F Dragan, Lucrezia Paterno Hotlzman, Nadeem Y Karimbux, Rebecca A Morin, Seyed Hossein Bassir
OBJECTIVES: This systematic review and meta-analysis aimed to compare clinical outcomes and width of keratinized tissue (KT) around teeth, following the soft tissue alter- natives and free gingival graft (FGG) procedures. The specific graft materials that were explored were extracellular matrix membrane, bilayer collagen membrane, living cellular construct, and acellular dermal matrix. METHODS: Four different databases were queried to identify human controlled clinical trials and randomized controlled clinical trials that fulfilled the eligibility criteria...
December 2017: Journal of Evidence-based Dental Practice
https://www.readbyqxmd.com/read/29184125/stiffness-dependent-motility-and%C3%A2-proliferation-uncoupled-by-deletion-of-cd44
#19
Ziba Razinia, Paola Castagnino, Tina Xu, Alexandra Vázquez-Salgado, Ellen Puré, Richard K Assoian
Information in the microenvironment guides complex cellular decisions such as whether or not to proliferate and migrate. The effects of soluble extracellular signals on these cellular functions are fairly well understood, but relatively little is known about how the extracellular matrix (ECM), and particularly the mechanical information in the ECM, guides these cellular decisions. Here, we show that CD44, a major receptor for the glycosaminoglycan ECM component hyaluronan, coordinates the motility and proliferative responses to ECM stiffening...
November 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29176036/one-step-delivery-of-a-functional-multi-layered-cell-sheet-using-a-thermally-expandable-hydrogel-with-controlled-presentation-of-cell-adhesive-proteins
#20
Yu Bin Lee, Joong-Yup Lee, Ha Yeon Byun, Taufiq Ahmad, Mitsuru Akashi, Michiya Matsusaki, Heungsoo Shin
In this study, we developed a new system enabling rapid delivery of a multi-layered cell sheet by combining layer-by-layer (LBL) coating of a cell membrane and surface engineered thermally expandable hydrogel. Human dermal fibroblasts were LBL-coated with fibronectin (FN) and gelatin to form a multi-layered cell sheet in a single seeding step via spontaneous 3D cell-cell interactions. FN was covalently immobilized onto the surface of a Tetronic®-based hydrogel at two different concentrations (1 and 5 g/ml) for stable adhesion of the multi-layered cell sheet, followed by polydopamine (PD) coating...
November 27, 2017: Biofabrication
keyword
keyword
51410
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"