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Extracellular Dermal Matrix

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https://www.readbyqxmd.com/read/28803240/impact-of-body-site-age-and-gender-on-the-collagen-elastin-index-by-noninvasive-in-vivo-vertical-two-photon-microscopy
#1
Carolin Czekalla, Karl-Heinz Schönborn, Nadine Döge, Sora Jung, Maxim E Darvin, Jürgen Lademann, Martina C Meinke
BACKGROUND/AIMS: Extrinsic and intrinsic skin aging is subject to constant remodeling and degradation processes, primarily in components of the extracellular matrix. While collagen fibers thin out during the aging process, the amorphous elastin fibers accumulate. These are essential formative components of the dermis. So far, these processes have been detected in vertical histological sections of invasive biopsies and recently in noninvasive horizontal scans. METHODS: In this pilot study, a modified noninvasive 2-photon microscope was applied to measure the collagen/elastin index of skin in vivo...
August 12, 2017: Skin Pharmacology and Physiology
https://www.readbyqxmd.com/read/28800110/response-of-dermal-fibroblasts-to-biochemical-and-physical-cues-in-aligned-polycaprolactone-silk-fibroin-nanofiber-scaffolds-for-application-in-tendon-tissue-engineering
#2
Chih-Hao Chen, Shih-Hsien Chen, Chang-Yi Kuo, Meng-Lun Li, Jyh-Ping Chen
Silk fibroin (SF) and fiber alignment were introduced into polycaprolactone (PCL)-based electrospun nanofibers as chemical and physical cues for tendon tissue engineering applications. The physicochemical properties of random PCL (RP) nanofibers, random PCL/SF (RPSF) nanofibers and aligned PCL/SF (APSF) nanofibers were characterized for fiber orientation and SF blending effects. An in vitro cell culture with rabbit dermal fibroblasts (RDFBs) on nanofibers indicated that SF promotes cell proliferation to a higher extent than fiber alignment...
August 11, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28795878/effects-of-prisma%C3%A2-skin-dermal-regeneration-device-containing-glycosaminoglycans-on-human-keratinocytes-and-fibroblasts
#3
Raffaella Belvedere, Valentina Bizzarro, Luca Parente, Francesco Petrella, Antonello Petrella
Prisma® Skin is a new pharmaceutical device developed by Mediolanum Farmaceutici S.p.a. It includes alginates, hyaluronic acid and mainly mesoglycan. The latter is a natural glycosaminoglycan preparation containing chondroitin sulfate, dermatan sulfate, heparan sulfate and heparin and it is used in the treatment of vascular disease. Glycosaminoglycans may contribute to the re-epithelialization in the skin wound healing, as components of the extracellular matrix. Here we describe, for the first time, the effects of Prisma® Skin in in vitro cultures of adult epidermal keratinocytes and dermal fibroblasts...
August 10, 2017: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/28771114/beneficial-effect-of-aligned-nanofiber-scaffolds-with-electrical-conductivity-for-the-directional-guide-of-cells
#4
Bon Kang Gu, Sang Jun Park, Chun-Ho Kim
Conducting polymer-based scaffolds receive biological and electrical signals from the extracellular matrix (ECM) or peripheral cells, thereby promoting cell growth and differentiation. Chitin, a natural polymer, is widely used as a scaffold because it is biocompatible, biodegradable, and nontoxic. In this study, we used an electrospinning technique to fabricate conductive scaffolds from aligned chitin/polyaniline (Chi/PANi) nanofibers for the directional guidance of cells. Pure chitin and random and aligned Chi/PANi nanofiber scaffolds were characterized using field emission scanning electron microscope (FE-SEM) and by assessing wettability, mechanical properties, and electrical conductivity...
August 3, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28770671/marine-algae-as-attractive-source-to-skin-care
#5
Jean-Yves Berthon, Rachida Nachat-Kappes, Mathieu Bey, Jean-Paul Cadoret, Isabelle Renimel, Edith Filaire
As the largest organ in the human body, the skin has multiple functions of which one of the most important is the protection against various harmful stressors. The keratinised stratified epidermis and an underlying thick layer of collagen-rich dermal connective tissues are important components of the skin. The environmental stressors such as ultraviolet radiation (UVR) and pollution increase the levels of reactive oxygen species (ROS), contributing to clinical manifestations such as wrinkle formation and skin aging...
June 2017: Free Radical Research
https://www.readbyqxmd.com/read/28760620/microcontact-printing-of-polydopamine-on-thermally-expandable-hydrogels-for-controlled-cell-adhesion-and-delivery-of-geometrically-defined-microtissues
#6
Yu Bin Lee, Se-Jeong Kim, Eum Mi Kim, Hayeon Byun, Hyung-Kwan Chang, Jungyul Park, Yu Suk Choi, Heungsoo Shin
Scaffold-free harvest of microtissue with a defined structure has received a great deal of interest in cell-based assay and regenerative medicine. In this study, we developed thermally expandable hydrogels with spatially controlled cell adhesive patterns for rapid harvest of geometrically controlled microtissue. We patterned polydopamine (PD) on to the hydrogel via microcontact printing (μCP), in linear shapes with widths of 50, 100 and 200 μm. The hydrogels facilitated formation of spatially controlled strip-like microtissue of human dermal fibroblasts (HDFBs)...
July 28, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28751213/evaluation-of-dermal-wound-healing-activity-of-synthetic-peptide-svvyglr
#7
Ayako Uchinaka, Naomasa Kawaguchi, Tsuyoshi Ban, Yoshinosuke Hamada, Seiji Mori, Yoshitaka Maeno, Yoshiki Sawa, Kohzo Nagata, Hirofumi Yamamoto
SVVYGLR peptide (SV peptide) is a 7-amino-acid sequence with angiogenic properties that is derived from osteopontin in the extracellular matrix and promotes differentiation of fibroblasts to myofibroblast-like cells and the production of collagen type Ⅲ by cardiac fibroblasts. However, the effects of SV peptide on dermal cells and tissue are unknown. In this study, we evaluated the effects of this peptide in a rat model of dermal wound healing. The synthetic SV peptide was added to dermal fibroblasts or keratinocytes, and their cellular motility was evaluated...
July 24, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28750552/human-skin-gene-expression-natural-trans-resveratrol-versus-five-resveratrol-analogs-for-dermal-applications
#8
Edwin D Lephart, Merritt B Andrus
Resveratrol (RV) is a polyphenolic compound naturally produced by plants. Polyphenolic compounds incorporated into medicinal products are beneficial but, RV is rapidly metabolized with an associated decline in biological activity. This study tested RV as the standard and compared five structurally modified RV analogs: butyrate, isobutyrate, palmitoate, acetate, and diacetate (to improve functionality) at 1% concentration(s) for 24 h in epiderm full thickness cultures by gene array/qPCR mRNA analysis. When silent mating type information regulation 2 homolog 1, extracellular elements (collagen1A1, 3A1, 4A1; elastin, tissue inhibitor of matrix metalloproteinase 1, fibrillin 1 laminin beta1 and matrix metalloproteinase 9), anti-aging and aging genes, inflammatory biomarkers (interleukin-1A [IL1A], IL1R2, IL-6 and IL-8), nerve growth factor, and the antioxidants (proliferating cell nuclear antigen, catalase, superoxide dismutase and metallothionein 1H/2H) were evaluated, ranking each from highest-to-lowest for gene expression: butyrate > isobutyrate > diacetate > acetate > palmitoate...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28742686/bioengineering-a-human-face-graft-the-matrix-of-identity
#9
Jérôme Duisit, Louis Maistriaux, Adriano Taddeo, Giuseppe Orlando, Virginie Joris, Emmanuel Coche, Catherine Behets, Jan Lerut, Chantal Dessy, Giulio Cossu, Esther Vögelin, Robert Rieben, Pierre Gianello, Benoît Lengelé
OBJECTIVE: During the last decade, face allotransplantation has been shown to be a revolutionary reconstructive procedure for severe disfigurements. However, offer to patients remains limited due to lifelong immunosuppression. To move forward in the field, a new pathway in tissue engineering is proposed. BACKGROUND: Our previously reported technique of matrix production of a porcine auricular subunit graft has been translated to a human face model. METHODS: 5 partial and 1 total face grafts were procured from human fresh cadavers...
July 24, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/28735299/identification-of-the-first-dominant-mutation-of-lama5-gene-causing-a-complex-multisystem-syndrome-due-to-dysfunction-of-the-extracellular-matrix
#10
Simone Sampaolo, Filomena Napolitano, Alfonsina Tirozzi, Mafalda Giovanna Reccia, Luca Lombardi, Olimpia Farina, Adriano Barra, Ferdinando Cirillo, Mariarosa Anna Beatrice Melone, Fernando Gianfrancesco, Giuseppe Di Iorio, Teresa Esposito
BACKGROUND: The laminin alpha 5 gene (LAMA5) plays a master role in the maintenance and function of the extracellular matrix (ECM) in mammalian tissues, which is critical in developmental patterning, stem cell niches, cancer and genetic diseases. Its mutations have never been reported in human disease so far. The aim of this study was to associate the first mutation in LAMA5 gene to a novel multisystem syndrome. METHODS: A detailed characterisation of a three-generation family, including clinical, biochemical, instrumental and morphological analysis, together with genetics and expression (WES and RNAseq) studies, was performed...
July 22, 2017: Journal of Medical Genetics
https://www.readbyqxmd.com/read/28730726/changes-in-the-extracellular-matrix-surrounding-human-chronic-wounds-revealed-by-2-photon-imaging
#11
Jessica E S Sutcliffe, Christopher Thrasivoulou, Thomas E Serena, Leigh Madden, Toby Richards, Anthony R J Phillips, David L Becker
Chronic wounds are a growing problem worldwide with no effective therapeutic treatments available. Our objective was to understand the composition of the dermal tissue surrounding venous leg ulcers and diabetic foot ulcers (DFU). We used novel 2-photon imaging techniques alongside classical histology to examine biopsies from the edges of two common types of chronic wound, venous leg ulcers and DFU. Compared to normal intact skin, we found that collagen levels are significantly reduced throughout the dermis of venous leg ulcer biopsies and DFU, with a reduction in both fibril thickness and abundance...
July 20, 2017: International Wound Journal
https://www.readbyqxmd.com/read/28711237/precise-role-of-dermal-fibroblasts-on-melanocyte-pigmentation
#12
REVIEW
Yinjuan Wang, Céline Viennet, Sophie Robin, Jean-Yves Berthon, Li He, Philippe Humbert
Dermal fibroblasts are traditionally recognized as synthesizing, remodeling and depositing collagen and extracellular matrix, the structural framework for tissues, helping to bring thickness and firmness to the skin. However, the role of fibroblasts on skin pigmentation arouses concern recently. More is known about the interactions between epidermal melanocytes and keratinocytes. This review highlights the importance of fibroblast-derived melanogenic paracrine mediators in the regulation of melanocyte activities...
July 1, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28697404/il-33-improves-wound-healing-through-enhanced-m2-macrophage-polarization-in-diabetic-mice
#13
Rongguo He, Hui Yin, Baohong Yuan, Tao Liu, Li Luo, Ping Huang, Liangcheng Dai, Kang Zeng
IL-33 is a newly discovered member of the IL-1 family and has been identified as a potent inducer of Th2 type immunity. Emerging evidence imply that IL-33 may also act as an alarm to alert the immune system when released by epithelial barrier tissues during trauma or infection. In this study, we further investigate the potential efficacy of IL-33 on dermal wound healing in streptozotocin-induced diabetic mice. A full-thickness skin wound was generated on the back of diabetic mice and treated with IL-33 or vehicle topically...
July 8, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28697219/the-role-of-a-natural-mollusk-egg-derived-ingredient-in-facial-appearance
#14
Zoe Diana Draelos
<p>New cosmeceutical ingredients that improve skin appearance are of interest to the dermatologist. Cryptomphalus aspersa is a snail raised on farms in Spain for its mucinous secretions and eggs. These natural products have been demonstrated in vitro to trigger mesenchymal stem cell differentiation, promote dermal fibroblast and keratinocyte migration, prevent keratinocyte aging, prevent oxidative damage, stimulate the extracellular matrix, and regulate MMPs. This 12-week study enrolled 40 male and female subjects age 40-70 years of Fitzpatrick skin types I-IV with moderate to severe facial aging and Rao-Goldman scores of 4-5 who applied an eye and face anti-aging cream twice daily containing a mollusk egg extract...
July 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28676875/fibroblast-fate-regulation-by-time-dependent-tgf-%C3%AE-1-and-il-10-stimulation-in-biomimetic-3d-matrices
#15
Jiranuwat Sapudom, Xiancheng Wu, Marina Chkolnikov, Michael Ansorge, Ulf Anderegg, Tilo Pompe
The presentation of TGF-β1 during the early stage of wound healing is a prerequisite for extracellular matrix (ECM) synthesis and remodeling by activated fibroblasts, called myofibroblasts. At later stages, clearance of myofibroblasts is needed to avoid overshooting ECM production. Apoptosis of myofibroblasts and the macrophage-released anti-inflammatory cytokine IL-10 are controversially discussed as regulating cues in this context. To reveal the regulating cues, defined biomaterial scaffolds are needed to conduct in-depth in vitro studies in a physiologically relevant context...
July 5, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28668298/interstitial-fluid-flow-induced-growth-potential-and-hyaluronan-synthesis-of-fibroblasts-in-a-fibroblast-populated-stretched-collagen-gel-culture
#16
Natsumi Saito, Hiroaki Adachi, Hiroshi Tanaka, Satoru Nakata, Norifumi Kawada, Ken Oofusa, Katsutoshi Yoshizato
BACKGROUND: Tensioned collagen gels with dermal fibroblasts (DFs) as a dermis model are usually utilized in a static culture (SC) that lacks medium flowing. To make the model closer to its in vivo state, we created a device to perfuse the model with media flowing at a physiological velocity and examined the effects of medium flow (MF) on the cultures. METHODS: We constructed a medium perfusion device for human DF-embedded stretched collagen gels (human dermis model), exposed the model to media that flows upwardly at ~1mL/day, and examined water retention of the gels, cells' growth ability, metabolic activity, expression profiles of nine extracellular matrix (ECM)-related genes...
September 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28629001/biomimetic-electrospun-scaffolds-from-main-extracellular-matrix-components-for-skin-tissue-engineering-application-the-role-of-chondroitin-sulfate-and-sulfated-hyaluronan
#17
Sirsendu Bhowmick, Sandra Rother, Heike Zimmermann, Poh S Lee, Stephanie Moeller, Matthias Schnabelrauch, Veena Koul, Rainer Jordan, Vera Hintze, Dieter Scharnweber
Incorporation of bioactive components like glycosaminoglycans (GAGs) into tissue engineering scaffolds, is a promising approach towards developing new generation functional biomaterial. Here, we have designed electrospun nanofibrous scaffolds made of gelatin and different concentrations of chemically sulfated or non-sulfated hyaluronan (sHA or HA) and chondroitin sulfate (CS). Evenly distributed fiber morphology was observed with no differences between varying concentrations and types of GAGs. In vitro release kinetics revealed that GAGs release is driven by diffusion...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28627622/human-amnion-derived-mesenchymal-stem-cells-protect-against-uva-irradiation-induced-human-dermal-fibroblast-senescence-in%C3%A2-vitro
#18
Chunli Zhang, Haishen Yuchi, Lu Sun, Xiaoli Zhou, Jinde Lin
The aim of the present study was to determine if human amnion‑derived mesenchymal stem cells (HAMSCs) exert a protective effect on ultraviolet A (UVA) irradiation-induced human dermal fibroblast (HDF) senescence. A senescence model was constructed as follows: HDFs (104‑106 cells/well) were cultured in a six‑well plate in vitro and then exposed to UVA irradiation at 9 J/cm2 for 30 min. Following the irradiation period, HDFs were co‑cultured with HAMSCs, which were seeded on transwells. A total of 72 h following the co‑culturing, senescence‑associated β‑galactosidase staining was performed and reactive oxygen species (ROS) content and mitochondrial membrane potential (Δψm) were detected in the HDFs via flow cytometric analysis...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28602628/overexpression-of-fibulin-5-attenuates-burn-induced-inflammation-via-trpv1-cgrp-pathway
#19
Xiaoqian Hou, Hui Li, Chunhua Zhang, Junling Wang, Xiuli Li, Xin Li
Fibulin-5, a multifunctional extracellular matrix protein, is up-regulated in response to mechanical injury and can promote dermal wound healing. This study aimed to explore the role and mechanism of Fibulin-5 in the pathogenesis of post-burn inflammation in thermally-injured mice. Here, we found that Fibulin-5 was up-regulated in the dorsal root ganglion (DRG) of burn-injured mice. After nociceptive behavioral testing, DRG was isolated and cultured to detect the mechanism of Fibulin-5 in thermal injury models by recombinant adenovirus overexpressing Fibulin-5, RT-qPCR, Western Blot, ELISA, AP20187 (an activator of one kind of kinase phosphorylating the α subunit of eukaryotic initiation factor 2, eIF2α), capsaicin (an agonist of transient receptor potential vanilloid (TRPV)-1), and an anti-CGRP neutralizing antibody...
August 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28598758/management-of-fournier-s-gangrene-non-healing-wounds-by-autologous-skin-micrograft-biotechnology-a-new-technique
#20
M A Bocchiotti, P Bogetti, A Parisi, F Rivarossa, A Frenello, E A Baglioni
Fournier's gangrene is an acute bacterial infection producing necrosis of the perineum and external genitalia that generally affects elderly men. Although skin grafts and flaps are the standard procedure for reconstruction, sometimes wounds can become chronic. Rigenera Protocol is a new technique based on autologous skin micrografts that reactivates and supports wound healing. A 40-year-old male with Fournier's gangrene, due to a rectal microperforation following diarrhoea, was treated with surgical debridement, negative pressure wound therapy and subsequently coverage with skin grafts...
June 2, 2017: Journal of Wound Care
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