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Extracellular Dermal Matrix

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https://www.readbyqxmd.com/read/28448819/construction-of-three-dimensional-vascularized-functional-human-liver-tissue-using-a-layer-by-layer-cell-coating-technique
#1
Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yasunari Fukuda, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Hiroshi Wada, Kunihito Gotoh, Koichi Kawamoto, Yuichiro Doki, Masaki Mori, Mitsuru Akashi
The creation of artificial liver tissue is an active area of research due to the shortage of donors for liver transplantation. Here we investigated whether a simple and efficient cell coating technique developed in our laboratory could be used to generate functional vascularized liver tissue. This technique creates three-dimensional tissue by loading cells sterically onto other cells that have been coated with layer-by-layer (LbL) nanofilms of fibronectin and gelatin, two extracellular matrix proteins. We used this technique to construct homogenous, dense, well-vascularized liver tissue from cryopreserved human primary hepatocytes, human umbilical vein endothelial cells, and normal human dermal fibroblasts...
February 28, 2017: Biomaterials
https://www.readbyqxmd.com/read/28448484/tissue-engineered-3d-human-lymphatic-microvascular-network-for-in-vitro-studies-of-lymphangiogenesis
#2
Laure Gibot, Todd Galbraith, Jennifer Bourland, Anita Rogic, Mihaela Skobe, François A Auger
This protocol describes a unique in vitro method for the generation of a 3D human lymphatic network within native connective tissue devoid of any exogenous material such as scaffolds or growth factors. In this five-stage protocol, human lymphatic endothelial cells (LECs) cocultured with dermal fibroblasts spontaneously organize into a stable 3D lymphatic capillary network. Stage 1 involves the isolation of primary fibroblasts and LECs from human skin. Fibroblasts are then cultured to produce connective tissue rich in extracellular matrix (stage 2), onto which LECs are seeded to form a network (stage 3)...
May 2017: Nature Protocols
https://www.readbyqxmd.com/read/28432900/lysophosphatidic-acid-induces-expression-of-genes-in-human-oral-keratinocytes-involved-in-wound-healing
#3
Hong Huynh Thorlakson, Stian Andre Engen, Olav Schreurs, Karl Schenck, Inger Johanne Schytte Blix
OBJECTIVE: Epithelial cells participate in wound healing by covering wounds, but also as important mediators of wound healing processes. Topical application of the phospholipid growth factor lysophosphatidic acid (LPA) accelerates dermal wound healing and we hypothesized that LPA can play a role in human oral wound healing through its effects on human oral keratinocytes (HOK). DESIGN: HOK were isolated from gingival biopsies and exposed to LPA. The LPA receptor profile, signal transduction pathways, gene expression and secretion of selected cytokines were analyzed...
April 13, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28422173/critical-involvement-of-zeb2-in-collagen-fibrillogenesis-the-molecular-similarity-between-mowat-wilson-syndrome-and-ehlers-danlos-syndrome
#4
Mika Teraishi, Mikiro Takaishi, Kimiko Nakajima, Mitsunori Ikeda, Yujiro Higashi, Shinji Shimoda, Yoshinobu Asada, Atsushi Hijikata, Osamu Ohara, Yoko Hiraki, Seiji Mizuno, Toshiyuki Fukada, Takahisa Furukawa, Nobuaki Wakamatsu, Shigetoshi Sano
Mowat-Wilson syndrome (MOWS) is a congenital disease caused by de novo heterozygous loss of function mutations or deletions of the ZEB2 gene. MOWS patients show multiple anomalies including intellectual disability, a distinctive facial appearance, microcephaly, congenital heart defects and Hirschsprung disease. However, the skin manifestation(s) of patients with MOWS has not been documented in detail. Here, we recognized that MOWS patients exhibit many Ehlers-Danlos syndrome (EDS)-like symptoms, such as skin hyperextensibility, atrophic scars and joint hypermobility...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28418608/methods-for-the-isolation-and-3d-culture-of-dermal-papilla-cells-from-human-hair-follicles
#5
H Topouzi, N J Logan, G Williams, C A Higgins
The dermal papilla is a cluster of mesenchymal cells located at the base of the hair follicle which have a number of important roles in the regulation of hair growth. As a consequence, in vitro models of these cells are widely used to study the molecular mechanisms which underlie hair follicle induction, growth and maintenance. While dermal papilla from rodent hair follicles can be digested prior to cell isolation, the unique extracellular matrix composition found in human dermal papilla renders enzymes such as trypsin and collagenase insufficient for digestion of the dermal papilla into a single cell suspension...
April 18, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28370945/alternatively-activated-macrophages-derived-from-thp-1-cells-promote-the-fibrogenic-activities-of-human-dermal-fibroblasts
#6
Zhensen Zhu, Jie Ding, Zengshuan Ma, Takashi Iwashina, Edward E Tredget
Macrophages play a key role in the wound healing process and can be divided into classically activated macrophages (M1) and alternatively activated macrophages (M2). Fibroblasts maintain the physical integrity of connective tissue, participate in wound closure as well as produce and remodel extracellular matrix. Macrophages have a close relationship with fibroblasts by increasing the production of matrix metalloproteinase-1 (MMP-1) for faster wound closure and remodeling and myofibroblast differentiation from fibroblasts...
April 3, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28370679/secreted-factors-from-equine-mesenchymal-stromal-cells-diminish-the-effects-of-tgf-%C3%AE-1-on-equine-dermal-fibroblasts-and-alter-the-phenotype-of-dermal-fibroblasts-isolated-from-cutaneous-fibroproliferative-wounds
#7
Rebecca M Harman, Ivanna V Bihun, Gerlinde R Van de Walle
The prevalence of cutaneous fibroproliferative disorders (CFPDs) is high and almost exclusively occurs in humans (keloids and hypertrophic scars) and horses (exuberant granulation tissue), making the horse a valuable translational model for studies on prevention and treatment of human CFPDs. CFPDs arise as a result of dysregulated wound healing characterized by persistently high levels of cytokines, such as transforming growth factor beta 1 (TGF-β1), that contribute to excessive extracellular matrix deposition, and the physical disorganization of dermal fibroblasts (DF)...
April 1, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28370536/the-impact-of-transcriptional-factor-fli1-deficiency-on-the-regulation-of-angiogenesis
#8
Tetsuo Toyama, Yoshihide Asano, Takuya Miyagawa, Kouki Nakamura, Megumi Hirabayashi, Takashi Yamashita, Ryosuke Saigusa, Shunsuke Miura, Yohei Ichimura, Takehiro Takahashi, Takashi Taniguchi, Ayumi Yoshizaki, Shinichi Sato
The insufficiency of Friend leukemia virus integration 1 (Fli1), a member of the Ets family transcription factors, is implicated in the pathogenesis of vasculopathy associated with systemic sclerosis (SSc). Fli1 deficiency accelerates early steps of angiogenesis, including detachment of pre-existing pericytes and extracellular matrix degradation by endothelial proteinases, but the impact of Fli1 deficiency on the other steps of angiogenesis has not been investigated. Therefore, we evaluated the effect of Fli1 deficiency on migration, proliferation, cell survival, and tube formation of human dermal microvascular endothelial cells (HDMECs)...
March 31, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28363295/biological-principles-of-scar-and-contracture
#9
REVIEW
Peter O Kwan, Edward E Tredget
Hypertrophic scar and contracture in burn patients is a complex process. Contributing factors include critical injury depth and activation of key cell subpopulations, including deep dermal fibroblasts, myofibroblasts, fibrocytes, and T-helper cells, which cause scarring rather than regeneration. These cells influence each other via cellular profibrotic and antifibrotic signals, which help to determine the outcome. These cells also both modify and interact with extracellular matrix of the wound, ultimately forming hypertrophic scar...
May 2017: Hand Clinics
https://www.readbyqxmd.com/read/28356446/fibroblast-specific-genetic-manipulation-of-p38-mapk-in-vivo-reveals-its-central-regulatory-role-in-fibrosis
#10
Jeffery D Molkentin, Darrian Bugg, Natasha Ghearing, Lisa E Dorn, Peter Kim, Michelle A Sargent, Jagadambika Gunaje, Kinya Otsu, Jennifer M Davis
Background -In the heart acute injury induces a fibrotic healing response that generates collagen rich scarring that is at first protective but if inappropriately sustained can worsen heart disease. The fibrotic process is initiated by cytokines, neuroendocrine effectors and mechanical strain that promote resident fibroblast differentiation into contractile and extracellular matrix producing myofibroblasts. The mitogen-activated protein kinase (MAPK) p38α (Mapk14 gene) is known to influence the cardiac injury response, but its direct role in orchestrating programmed fibroblast differentiation and fibrosis in vivo is unknown...
March 29, 2017: Circulation
https://www.readbyqxmd.com/read/28350869/in-vitro-skin-models-to-study-epithelial-regeneration-from-the-hair-follicle
#11
Nkemcho Ojeh, Baki Akgül, Marjana Tomic-Canic, Mike Philpott, Harshad Navsaria
The development of dermal equivalents (DEs) for the treatment of burns has contributed toward efficient wound closure. A collagen-glycosaminoglycan DE (C-GAG) grafted with hair follicles converted a full-thickness wound to partial-thickness resulting in complete wound closure and restored hair. In this study we compared the ability of both intact pilosebaceous units (PSU) or truncated hair follicles (THF) to regenerate a multilayered epidermis in vitro when implanted into de-epidermalized dermis (DED) or C-GAG with the epidermis generated in vivo using C-CAG...
2017: PloS One
https://www.readbyqxmd.com/read/28350069/phytosphingosine-1-phosphate-and-epidermal-growth-factor-synergistically-restore-extracellular-matrix-in-human-dermal-fibroblasts-in-vitro-and-in-vivo
#12
Seung Bin Kwon, Sungkwan An, Min Jung Kim, Ka Ram Kim, Young Min Choi, Kyu Joong Ahn, In-Sook An, Hwa Jun Cha
Phytosphingosine-1-phosphate (PhS1P), which is found in plants and fungi, is generated by the phosphorylation of phytosphingosine and is structurally similar to molecules that promote cellular growth and proliferation. The aim of this study was to ascertain whether PhS1P displays synergistic effects together with epidermal growth factor (EGF), which is also critical for activating proliferation, migration and survival pathways. We utilized cultured human dermal fibroblasts (HDFs) and a number of assays, including western blotting, cell migration assays, quantitative (real-time) PCR, and viability assays...
January 23, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28335694/construction-of-three-dimensional-dermo-epidermal-skin-equivalents-using-cell-coating-technology-and-their-utilization-as-alternative-skin-for-permeation-studies-and-skin-irritation-tests
#13
Takami Akagi, Mayuka Nagura, Ayami Hiura, Hajime Kojima, Mitsuru Akashi
In vitro generated human skin equivalents are generating interest as promising tools in basic research, as alternatives to animal testing and for clinical applications in regenerative medicine. For prediction of skin irritation and corrosion, three-dimensional (3D) human skin equivalents consisting of differentiated human keratinocytes have been developed and some models have been internationally accepted. However, more delicate assessments using full-thickness skin models, such as skin sensitization tests cannot be performed because of the lack of a dermis containing fibroblasts or appendages...
March 23, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28333992/improved-epidermal-barrier-formation-in-human-skin-models-by-chitosan-modulated-dermal-matrices
#14
Arnout Mieremet, Marion Rietveld, Samira Absalah, Jeroen van Smeden, Joke A Bouwstra, Abdoelwaheb El Ghalbzouri
Full thickness human skin models (FTMs) contain an epidermal and a dermal equivalent. The latter is composed of a collagen dermal matrix which harbours fibroblasts. Current epidermal barrier properties of FTMs do not fully resemble that of native human skin (NHS), which makes these human skin models less suitable for barrier related studies. To further enhance the resemblance of NHS for epidermal morphogenesis and barrier formation, we modulated the collagen dermal matrix with the biocompatible polymer chitosan...
2017: PloS One
https://www.readbyqxmd.com/read/28323545/creation-of-a-bioengineered-skin-flap-scaffold-with-a-perfusable-vascular-pedicle
#15
Bernhard J Jank, Jeremy Goverman, Jacques P Guyette, Jon M Charest, Mark A Randolph, Glenn R Gaudette, Joshua R Gershlak, Martin Purschke, Emilia Javorsky, Rosalynn M Nazarian, David A Leonard, Curtis L Cetrulo, William G Austen, Harald Christian Ott
Full thickness skin loss is a challenging problem due to limited reconstructive options, demanding 75 million surgical procedures annually in the United States. Autologous skin grafting is the gold standard treatment, but results in donor site morbidity and poor aesthetics. Numerous skin substitutes are available on the market to date, however, none truly function as full thickness skin due to their lack of a vascular network. The creation of an autologous full thickness skin analogue with a vascular pedicle would result in a paradigm shift in the management of wounds and in reconstruction of full thickness skin defects...
March 21, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28323389/atomic-force-microscopy-in-the-production-of-a-biovital-skin-graft-based-on-human-acellular-dermal-matrix-produced-in-house-and-in-vitro-cultured-human-fibroblasts
#16
Wojciech Łabuś, Justyna Glik, Agnieszka Klama-Baryła, Diana Kitala, Małgorzata Kraut, Mariusz Maj, Mariusz Nowak, Marcelina Misiuga, Andrzej Marcinkowski, Barbara Trzebicka, Ryszard Poloczek, Marek Kawecki
The most efficient method in III° burn treatment is the use of the autologous split thickness skin grafts that were donated from undamaged body area. The main limitation of this method is lack of suitable donor sites. Tissue engineering is a useful tool to solve this problem. The goal of this study was to find the most efficient way of producing biovital skin substitute based on in house produced acellular dermal matrix ADM and in vitro cultured fibroblasts. Sixty samples of sterilized human allogeneic skin (that came from 10 different donors) were used to examine the influence of decellularizing substances on extracellular matrix and clinical usefulness of the test samples of allogeneic human dermis...
March 21, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28297581/systemic-delivery-of-bone-marrow-mesenchymal-stem-cells-for-in-situ-intervertebral-disc-regeneration
#17
Carla Cunha, Catarina R Almeida, Maria Inês Almeida, Andreia M Silva, Maria Molinos, Sofia Lamas, Catarina L Pereira, Graciosa Q Teixeira, António T Monteiro, Susana G Santos, Raquel M Gonçalves, Mário A Barbosa
Cell therapies for intervertebral disc (IVD) regeneration presently rely on transplantation of IVD cells or stem cells directly to the lesion site. Still, the harsh IVD environment, with low irrigation and high mechanical stress, challenges cell administration and survival. In this study, we addressed systemic transplantation of allogeneic bone marrow mesenchymal stem cells (MSCs) intravenously into a rat IVD lesion model, exploring tissue regeneration via cell signaling to the lesion site. MSC transplantation was performed 24 hours after injury, in parallel with dermal fibroblasts as a control; 2 weeks after transplantation, animals were killed...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28288846/pleiotropic-fty720-is-a-specific-and-potent-therapy-for-hypertrophic-scars
#18
Fen Shi, Xiaoling Cao, Zhicheng Hu, Da Ma, Dong Guo, Jian Zhang, Changlin Zhang, Peng Liu, Shanqiang Qu, Jiayuan Zhu, Wuguo Deng, Bing Tang
Hypertrophic scars (HS) is a fibrotic skin condition characterized by aberrant fibroblast phenotypes and excessive deposition of extracellular matrix components. 2-Amino-2-[2-(4-octylphenyl)]-1, 3-propanediol hydrochloride (FTY720), an immunomodulator approved for treating multiple sclerosis, is reported to attenuate fibrosis in multiple disease models. Here we found that FTY720 could significantly attenuate the proliferation and fibrosis in HS fibroblasts (HSFs) as well as in animal HS model. Upon treating HSFs or normal dermal fibroblasts (NFs) with FTY720 at different concentrations for different time periods, we found that FTY720 presented a pleiotropic effect specifically on HSFs, but not NFs, including reducing cell viability, arresting cell cycle progression at G0/G1 phase, promoting apoptosis, inhibiting migration and contraction, and suppressing the expressions of α-smooth muscle actin (α-SMA), collagen I, and collagen III...
March 10, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28267785/smad3-dependent-ccn2-mediates-fibronectin-expression-in-human-skin-dermal-fibroblasts
#19
Trupta Purohit, Zhaoping Qin, Chunji Quan, Zhenhua Lin, Taihao Quan
The potential involvement of connective tissue growth factor (CCN2/CTGF) in extracellular matrix (ECM) production is recognized. However, the role CCN2 in fibronectin (FN) gene expression has remained incompletely understood and even controversial. Here we report that CCN2 is absolutely necessary for FN expression in primary human skin dermal fibroblasts, the major cells responsible for ECM production in skin. Gain- and loss-of-function approaches demonstrate that CCN2 is an essential component of FN expression in both basal and stimulation by TGF-β signaling, the major regulator of FN expression...
2017: PloS One
https://www.readbyqxmd.com/read/28251072/novel-technique-for-a-symptomatic-subscapularis-herniation-through-a-scapu-lar-defect
#20
Luis Grau, Kevin Chen, Ali A Alhandi, Benjamin Goldberg
Fractures of the scapula are rare and have been reported to account for only 1% of all fractures and 3%-5% of upper extremity fractures. Several studies have reported successful outcomes with non-operative treatment of scapula fractures. Although non-operative treatments are successful in a very high percentage of patients, very few cases of non-union of scapular body fractures have been reported. In our review of the literature, we found two case reports of scapular body fractures developed into non-unions...
February 18, 2017: World Journal of Orthopedics
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