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Extracellular Dermal Matrix

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https://www.readbyqxmd.com/read/28063210/the-progress-and-challenges-for-dermal-regeneration-in-tissue-engineering
#1
REVIEW
Hanlei Zhou, Chuangang You, Ronghua Jin, Pan Wu, Qiong Li, Chunmao Han
Wound healing is an inherent response resulting in the restoration of tissue integrity. It is a complex process involving cell migration, proliferation, differentiation, apoptosis, and the synthesis and remodeling of the extracellular matrix (ECM). The dermal tissue is an important component of skin that acts as a connecting link between the epidermis and hypodermis. The appearance of scars and contractures after autologous split-thickness skin transplantation or single epidermis diaphragm transplantation for full skin defects indicates that the dermal tissue plays an important role in skin regeneration...
January 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28041746/evaluation-of-cultured-human-dermal-and-dermo-epidermal-substitutes-focusing-on-extracellular-matrix-components-comparison-of-protein-and-rna-analysis
#2
Corien Oostendorp, Sarah Meyer, Monia Sobrio, Joyce van Arendonk, Ernst Reichmann, Willeke F Daamen, Toin H van Kuppevelt
Treatment of full-thickness skin defects with split-thickness skin grafts is generally associated with contraction and scar formation and cellular skin substitutes have been developed to improve skin regeneration. The evaluation of cultured skin substitutes is generally based on qualitative parameters focusing on histology. In this study we focused on quantitative evaluation to provide a template for comparison of human bio-engineered skin substitutes between clinical and/or research centers, and to supplement histological data...
December 29, 2016: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28038939/skin-fragility-in-the-wild-derived-inbred-mouse-strain-mus-pahari-eij
#3
C Herbert Pratt, Christopher S Potter, Thomas J Sproule, Raoul V Kuiper, Son Yong Karst, Soheil S Dadras, Derry C Roopenian, John P Sundberg
Mus pahari is a wild-derived, inbred mouse strain. M. pahari colony managers observed fragility of this strain's skin resulting in separation of tail skin from the mouse if handled incorrectly. Tail skin tension testing of M. pahari resulted in significantly lowered force threshold for caudal skin rupture and loss in comparison to closely related inbred mouse species and subspecies and even more than a model for junctional epidermolysis bullosa. Histologically, the tail skin separated at the subdermal level with the dermis firmly attached to the epidermis, excluding the epidermolysis bullosa complex of diseases...
December 27, 2016: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28034554/biological-characters-of-human-dermal-fibroblasts-derived-from-foreskin-of-male-infertile-patients
#4
Fang Fang, Ke Ni, Yiting Cai, Zhen Ye, Jin Shang, Shiliang Shen, Chengliang Xiong
Dermal fibroblasts play a vital role in maintaining skin function. They not only synthesize and secrete extracellular matrix molecules, but also produce a complex mixture of bioactive factors, which both contribute to immune regulation and wound healing. Fibroblasts isolated from skin tissue exhibit wide range of potentials, especially in regenerative medicine. The use of fibroblast cultures for medical purposes requires standardization of cell preparations. To achieve this, we isolated and characterized dermal fibroblasts from human foreskin with a standardized method...
December 8, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/28033645/the-facial-adipose-tissue-a-revision
#5
Ilja Kruglikov, Oscar Trujillo, Quick Kristen, Kerelos Isac, Julia Zorko, Maria Fam, Kasie Okonkwo, Asima Mian, Hyunh Thanh, Konstantin Koban, Anthony P Sclafani, Hanno Steinke, Sebastian Cotofana
Recent advantages in the anatomical understanding of the face have turned the focus toward the subcutaneous and deep facial fat compartments. During facial aging, these fat-filled compartments undergo substantial changes along with other structures in the face. Soft tissue filler and fat grafting are valid methods to fight the signs of facial aging, but little is known about their precise effect on the facial fat. This narrative review summarizes the current knowledge about the facial fat compartments in terms of anatomical location, histologic appearance, immune-histochemical characteristics, cellular interactions, and therapeutic options...
December 2016: Facial Plastic Surgery: FPS
https://www.readbyqxmd.com/read/28027929/nrf2-exerts-cell-autonomous-antifibrotic-effects-compromised-function-in-systemic-sclerosis-and-therapeutic-rescue-with-a-novel-heterocyclic-chalcone-derivative
#6
Jun Wei, Hongyan Zhu, Gabriel Lord, Mitra Bhattachayya, Brielle M Jones, Graham Allaway, Shyam S Biswal, Benjamin Korman, Roberta G Marangoni, Warren G Tourtellotte, John Varga
The transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) governs antioxidant, innate immune and cytoprotective responses and its deregulation is prominent in chronic inflammatory conditions. To examine the hypothesis that Nrf2 might be implicated in systemic sclerosis (SSc), we investigated its expression, activity, and mechanism of action in SSc patient samples and mouse models of fibrosis and evaluated the effects of a novel pharmacologic Nrf2 agonist. We found that both expression and activity of Nrf2 were significantly reduced in SSc patient skin biopsies and showed negative correlation with inflammatory gene expression...
December 9, 2016: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/28009972/inhibition-of-the-hedgehog-pathway-leads-to-antifibrotic-effects-in-dermal-fibrosis
#7
Hua-Li Cao, Jiong Zhou, Xi-Bei Chen, Lilla Landeck, Jian-Qiang Yang, Jia-Qi Chen, Wei Li, Sui-Qing Cai, Min Zheng, Xiao-Yong Man
Dermal fibrosis is characterized by the activation of the matrix-producing 'positive' myofibroblasts, and the relentless production and deposition of extracellular matrix. The hedgehog pathway has recently been demonstrated to work in a pro-fibrotic manner in systemic sclerosis (SSc). A negative regulator of the hedgehog pathway (Hh), the suppressor of fused (Sufu), was shown to be involved in the activation of fibrotic diseases. However, the exact role of Sufu in fibrosis has not been investigated so far. In our study, we aimed to define the role of sufu in the process of fibrosis using dermal fibroblasts of healthy donors that were cultured in vitro...
November 2016: Discovery Medicine
https://www.readbyqxmd.com/read/27999795/in-vivo-remodeling-of-fibroblast-derived-vascular-scaffolds-implanted-for-6-months-in-rats
#8
Maxime Y Tondreau, Véronique Laterreur, Karine Vallières, Robert Gauvin, Jean-Michel Bourget, Catherine Tremblay, Dan Lacroix, Lucie Germain, Jean Ruel, Francois A Auger
There is a clinical need for tissue-engineered small-diameter (<6 mm) vascular grafts since clinical applications are halted by the limited suitability of autologous or synthetic grafts. This study uses the self-assembly approach to produce a fibroblast-derived decellularized vascular scaffold (FDVS) that can be available off-the-shelf. Briefly, extracellular matrix scaffolds were produced using human dermal fibroblasts sheets rolled around a mandrel, maintained in culture to allow for the formation of cohesive and three-dimensional tubular constructs, and decellularized by immersion in deionized water...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27993221/exendin-4-a-glucagon-like-peptide-1-analogue-accelerates-diabetic-wound-healing
#9
Jun-Neng Roan, Han-Ni Cheng, Chao-Chung Young, Chi-Ju Lee, Ming-Long Yeh, Chwan-Yau Luo, Yau-Sheng Tsai, Chen-Fuh Lam
BACKGROUND: Diabetes disregulates inflammatory responses and impairs vascular function in wounds. Glucagon-like peptide-1 receptor (Glp-1R) agonists are hypoglycemic agents with pleiotropic vascular protective and anti-inflammatory effects. The therapeutic potential of a Glp-1 analogue in a diabetic rat model of excisional wound injury was investigated. MATERIALS AND METHODS: Excisional wounds were created on the dorsum of streptozotocin-induced diabetic rats, which received placebo or Glp-1 analogue exendin-4 (Ex4; 0...
February 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/27965915/mixed-fibronectin-derived-peptides-conjugated-to-a-chitosan-matrix-effectively-promotes-biological-activities-through-integrins-%C3%AE-4%C3%AE-1-%C3%AE-5%C3%AE-1-%C3%AE-v%C3%AE-3-and-syndecan
#10
Kentaro Hozumi, Kyotaro Nakamura, Haruna Hori, Mari Miyagi, Rika Nagao, Keiko Takasaki, Fumihiko Katagiri, Yamato Kikkawa, Motoyoshi Nomizu
Mimicking the biological function of the extracellular matrix is an approach to developing cell adhesive biomaterials. The RGD peptide, derived from fibronectin (Fn), mainly binds to integrin αvβ3 and has been widely used as a cell adhesive peptide on various biomaterials. However, cell adhesion to Fn is thought to be mediated by several integrin subtypes and syndecans. In this study, we synthesized an RGD-containing peptide (FIB1) and four integrin α4β1-binding-related motif-containing peptides (LDV, IDAPS, KLDAPT, and PRARI) and constructed peptide-chitosan matrices...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27942228/reactivating-the-extracellular-matrix-synthesis-of-sulfated-glycosaminoglycans-and-proteoglycans-to-improve-the-human-skin-aspect-and-its-mechanical-properties
#11
Hanane Chajra, Daniel Auriol, Francine Joly, Aurélie Pagnon, Magda Rodrigues, Sophie Allart, Gérard Redziniak, Fabrice Lefevre
BACKGROUND: The aim of this study was to demonstrate that a defined cosmetic composition is able to induce an increase in the production of sulfated glycosaminoglycans (sGAGs) and/or proteoglycans and finally to demonstrate that the composition, through its combined action of enzyme production and synthesis of macromolecules, modulates organization and skin surface aspect with a benefit in antiaging applications. MATERIALS AND METHODS: Gene expression was studied by quantitative reverse transcription polymerase chain reaction using normal human dermal fibroblasts isolated from a 45-year-old donor skin dermis...
2016: Clinical, Cosmetic and Investigational Dermatology
https://www.readbyqxmd.com/read/27939785/inhibition-of-collagen-synthesis-by-iwr-1-in-normal-and-keloid-derived-skin-fibroblasts
#12
Ming-Wei Zhou, Wei-Tian Yin, Ri-Hua Jiang, Jin-Hyup Lee, Chang-Deok Kim, Jeung-Hoon Lee, Ming Ji Zhu, Tae-Jin Yoon
AIMS: Keloid is a benign tumor that is characterized by the hyperproliferation of dermal fibroblasts and excessive deposition of extracellular matrix (ECM) especially the collagen. Aberrant activation of Wnt/β-catenin signaling is implicated in the pathogenesis of keloid. In this study, we investigated the effects of IWR-1, a small molecule inhibitor for Wnt/β-catenin signaling via the inhibition of tankyrase, on production of collagen and matrix metalloproteinase (MMP) in dermal fibroblasts...
December 7, 2016: Life Sciences
https://www.readbyqxmd.com/read/27935551/organotypic-soft-tissue-co-cultures-morphological-changes-in-microvascular-endothelial-tubes-after-incubation-with%C3%A2-iodinated-contrast-media
#13
S Kaessmeyer, J Sehl, M Khiao In, B Hiebl, R Merle, F Jung, R P Franke, J Plendl
INTRODUCTION: Clinical complications like thrombosis or anaphylaxis have been described to go along with the intra-venous or intra-arterial injection of iodinated contrast media (CM). It has been suggested that the administration of CM affects rheological parameters and thereby causes reduced blood velocity in microvessels. In vitro studies revealed significant buckling of endothelial cells after exposure to CM reducing the lumen of vessels. The aim of this study was to test the influence of CM on three-dimensional microvascular tubules with open lumina within an organotypic soft-tissue co-culture assay in vitro...
December 8, 2016: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/27933737/testing-the-influence-of-surfactant-based-wound-dressings-on-proteinase-activity
#14
Sunyoung Jeong, Gregory S Schultz, Daniel J Gibson
Proteinases are enzymes that can digest other proteins. In chronic wounds, a sub-class of these enzymes with the ability to degrade the extracellular matrix (matrix metalloproteinases, MMPs) have been found to both inhibit healing and to be able to aid in enzymatically debriding a wound. Enzymatic debridement using the enzymes present in a wound is generally called autolytic debridement. Clinicians seeking to employ autolytic debridement typically use occlusive materials such as medical honey, alginate dressings and other occlusive dressings...
December 8, 2016: International Wound Journal
https://www.readbyqxmd.com/read/27909667/arthroscopic-capsular-reconstruction-of-the-hip-with-acellular-dermal-extracellular-matrix-surgical-technique
#15
Itay Perets, David E Hartigan, John P Walsh, Edwin Chaharbakhshi, Mary R Close, Benjamin G Domb
Atraumatic instability of the hip has become an increasingly studied occurrence in recent years. There are several established surgical techniques that help restore stability of the native hip joint. In some cases, these procedures are not an option. As the phenomenon has become recognized more frequently, a greater number of revision surgeries are warranted in patients with ligamentous laxity. A durable solution for irreparable microinstability needs to be formulated to address this vulnerable patient demographic...
October 2016: Arthroscopy Techniques
https://www.readbyqxmd.com/read/27909082/proteomic-investigation-of-dermal-fibroblasts-isolated-from-affected-and-unaffected-skin-samples-from-patients-with-limited-cutaneous-systemic-sclerosis-2-distinct-entities
#16
Claudio Corallo, Annalisa Santucci, Giulia Bernardini, Natale Figura, Roberto Leoncini, Giulia Riolo, Antonio Montella, Chiara Chirico, Ranuccio Nuti, Nicola Giordano
OBJECTIVE: To identify using proteomic analysis the proteins of altered abundance in the affected and unaffected limited cutaneous systemic sclerosis (lcSSc) skin fibroblasts. METHODS: Excision biopsies (3 mm) were obtained from the affected and unaffected skin of 5 patients with lcSSc. Dermal fibroblasts were isolated enzymatically. Two-dimensional gel electrophoresis was used to separate and define proteins in affected and unaffected fibroblast lysates. Proteins of altered abundance were identified by mass spectrometry...
January 2017: Journal of Rheumatology
https://www.readbyqxmd.com/read/27892645/endothelial-cell-derived-endothelin-1-is-involved-in-abnormal-scar-formation-by-dermal-fibroblasts-through-rhoa-rho-kinase-pathway
#17
Koichiro Kiya, Tateki Kubo, Kenichiro Kawai, Shinsuke Matsuzaki, Rieko Muramatsu, Daisuke Maeda, Toshihiro Fujiwara, Akimitsu Nishibayashi, Shigeyuki Kanazawa, Kenji Yano, Genki Amano, Taiichi Katayama, Ko Hosokawa
Hypertrophic scars and keloids are characterized by excessive dermal deposition of extracellular matrix due to fibroblast-to-myofibroblast differentiation. Endothelin-1 (ET-1) is primarily produced by vascular endothelial cells, and plays multiple roles in the wound-healing response and organ fibrogenesis. In this study, we investigated the pathophysiological significance of ET-1 and involvement of RhoA, a member of the Rho GTPases, in hypertrophic scar/keloid formation. We found that ET-1 expression on dermal microvascular endothelial cells (ECs) in hypertrophic scars and keloids was higher than that in normal skin and mature scars...
November 28, 2016: Experimental Dermatology
https://www.readbyqxmd.com/read/27888134/perilla-frutescens-leaves-extract-ameliorates-ultraviolet-radiation-induced-extracellular-matrix-damage-in-human-dermal-fibroblasts-and-hairless-mice-skin
#18
Jung-Soo Bae, Mira Han, Hee Soon Shin, Min-Kyoung Kim, Chang-Yup Shin, Dong Hun Lee, Jin Ho Chung
ETHNOPHARMACOLOGICAL RELEVANCE: Perilla frutescens (L.) Britt. (Lamiaceae) is a traditional herb that is consumed in East Asian countries as a traditional medicine. This traditional herb has been documented for centuries to treat various diseases such as depression, allergies, inflammation and asthma. However, the effect of Perilla frutescens on skin has not been characterized well. AIM OF THE STUDY: The present study aimed to investigate the effect of Perilla frutescens leaves extract (PLE) on ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin...
January 4, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27877059/skin-rejuvenation-using-cosmetic-products-containing-growth-factors-cytokines-and-matrikines-a-review-of-the-literature
#19
REVIEW
Caroline Aldag, Diana Nogueira Teixeira, Phillip S Leventhal
Skin aging is primarily due to alterations in the dermal extracellular matrix, especially a decrease in collagen I content, fragmentation of collagen fibrils, and accumulation of amorphous elastin material, also known as elastosis. Growth factors and cytokines are included in several cosmetic products intended for skin rejuvenation because of their ability to promote collagen synthesis. Matrikines and matrikine-like peptides offer the advantage of growth factor-like activities but better skin penetration due to their much smaller molecular size...
2016: Clinical, Cosmetic and Investigational Dermatology
https://www.readbyqxmd.com/read/27867754/a-small-chimeric-fibronectin-fragment-accelerates-dermal-wound-repair-in-diabetic-mice
#20
Denise C Hocking, James R Brennan, Carol H Raeman
Objective: During wound repair, soluble fibronectin is converted into biologically active, insoluble fibrils via a cell-mediated process. This fibrillar, extracellular matrix (ECM) form of fibronectin stimulates cell processes critical to tissue repair. Nonhealing wounds show reduced levels of ECM fibronectin fibrils. The objective of this study was to produce a small, recombinant wound supplement with the biological activity of insoluble fibronectin fibrils. Approach: A chimeric fibronectin fragment was produced by inserting the integrin-binding Arg-Gly-Asp (RGD) loop from the tenth type III repeat of fibronectin (FNIII10) into the analogous site within the heparin-binding, bioactive fragment of the first type III repeat (FNIII1H)...
November 1, 2016: Advances in Wound Care
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