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Extracellular Dermal Matrix

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https://www.readbyqxmd.com/read/28529754/chitosan-poly-caprolactone-nanofibers-for-skin-repair
#1
Sheeny Lan Levengood, Ariane E Erickson, Fei-Chien Chang, Miqin Zhang
Dermal wounds, both acute and chronic, represent a significant clinical challenge and therefore the development of novel biomaterial-based skin substitutes to promote skin repair is essential. Nanofibers have garnered attention as materials to promote skin regeneration due to the similarities in morphology and dimensionality between nanofibers and native extracellular matrix proteins, which are critical in guiding cutaneous wound healing. Electrospun chitosan-poly(caprolactone) (CPCL) nanofiber scaffolds, which combine the important intrinsic biological properties of chitosan and the mechanical integrity and stability of PCL, were evaluated as skin tissue engineering scaffolds using a mouse cutaneous excisional skin defect model...
March 7, 2017: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://www.readbyqxmd.com/read/28498357/adiponectin-is-involved-in-connective-tissue-growth-factor-induced-proliferation-migration-and-overproduction-of-the-extracellular-matrix-in-keloid-fibroblasts
#2
Limin Luo, Jun Li, Han Liu, Xiaoqing Jian, Qianlei Zou, Qing Zhao, Qu Le, Hongdou Chen, Xinghua Gao, Chundi He
Adiponectin, an adipocyte-derived hormone, exerts pleiotropic biological effects on metabolism, inflammation, vascular homeostasis, apoptosis and immunity. Recently, adiponectin has been suggested to attenuate the progression of human dermal fibrosis. Connective tissue growth factor (CTGF) is induced in keloids and is thought to be participated in the formation of keloid fibrosis. However, the roles played by adiponectin in keloids remain unclear. In this study, we explored the effects of adiponectin on CTGF-induced cell proliferation, migration and the deposition of extracellular matrix (ECM) and their associated intracellular signalling pathways in keloid fibroblasts (KFs)...
May 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28493850/importance-of-endogenous-extracellular-matrix-in-biomechanical-properties-of-human-skin-model
#3
Flavien Pillet, Laure Gibot, Moinecha Madi, Marie-Pierre Rols, Etienne Dague
The physical and mechanical properties of cells modulate their behavior such proliferation rate, migration and extracellular matrix remodeling. In order to study cell behavior in a tissue-like environment in vitro, it is of utmost importance to develop biologically and physically relevant 3D cell models. Here, we characterized the physical properties of a single cell type growing in configurations of increasing complexity. From one human skin biopsy, primary dermal fibroblasts were isolated and seeded to give monolayer (2D model), spheroid (3D model poor in extracellular matrix) and tissue-engineered cell sheet (3D model rich in endogenous extracellular matrix)...
May 11, 2017: Biofabrication
https://www.readbyqxmd.com/read/28489283/inflammatory-responses-matrix-remodeling-and-re-epithelialization-after-fractional-co2-laser-treatment-of-scars
#4
Danielle M DeBruler, Britani N Blackstone, Molly E Baumann, Kevin L McFarland, Brian C Wulff, Traci A Wilgus, J Kevin Bailey, Dorothy M Supp, Heather M Powell
BACKGROUND AND OBJECTIVE: Fractional CO2 laser therapy has been used to improve scar pliability and appearance; however, a variety of treatment protocols have been utilized with varied outcomes. Understanding the relationship between laser power and extent of initial tissue ablation and time frame for remodeling could help determine an optimum power and frequency for laser treatment. The characteristics of initial injury caused by fractional CO2 laser treatment, the rates of dermal remodeling and re-epithelialization, and the extent of inflammation as a function of laser stacking were assessed in this study in a porcine scar model...
May 10, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28488903/versican-a-subdomain-is-required-for-its-adequate-function-in-dermal-development
#5
Sonoko Hatano, Naoko Nagai, Nobuo Sugiura, Jun Tsuchimoto, Zenzo Isogai, Koji Kimata, Akinobu Ota, Sivasundaram Karnan, Yoshitaka Hosokawa, Hideto Watanabe
Versican, a large chondroitin sulfate proteoglycan, serves as a structural macromolecule of the extracellular matrix and regulates cell behavior. We determined the function of versican in dermal development using Vcan(Δ3/Δ3) mutant mice expressing versican with deleted A-subdomain of the N-terminal G1 domain. The mutant versican showed a decreased hyaluronan-binding ability and failed to accumulate in the extracellular matrix. In the early developmental stage, Vcan(Δ3/Δ3) dermis showed a decrease in versican expression as compared with WT...
May 10, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28477344/artocarpin-enriched-artocarpus-altilis-heartwood-extract-protects-against-uvb-induced-mechanical-damage-in-dermal-fibroblasts
#6
Narisara Tiraravesit, Philippe Humbert, Sophie Robin, Marion Tissot, Céline Viennet, Jarupa Viyoch
Ultraviolet B radiation has a negative effect on the mechanical properties of the dermis and on the activities of resident fibroblasts, including the disorganization and fragmentation of the dermal extracellular matrix (ECM). It induces alterations in cytoskeleton structure, motility and the contractile forces of the fibroblasts. The present study aimed to evaluate the protective effect of Artocarpus altilis heartwood extract on the mechanical properties of ultraviolet B-irradiated fibroblasts. Human skin fibroblasts were pretreated with 50 μg/mL artocarpin-enriched extract for 48 h and later irradiated with ultraviolet B at 200 mJ/cm(2) ...
May 6, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28475575/age-gender-and-uv-exposition-related-effects-on-gene-expression-in-in-vivo-aged-short-term-cultivated-human-dermal-fibroblasts
#7
Wolfgang Kaisers, Petra Boukamp, Hans-Jürgen Stark, Holger Schwender, Julia Tigges, Jean Krutmann, Heiner Schaal
Ageing, the progressive functional decline of virtually all tissues, affects numerous living organisms. Main phenotypic alterations of human skin during the ageing process include reduced skin thickness and elasticity which are related to extracellular matrix proteins. Dermal fibroblasts, the main source of extracellular fibrillar proteins, exhibit complex alterations during in vivo ageing and any of these are likely to be accompanied or caused by changes in gene expression. We investigated gene expression of short term cultivated in vivo aged human dermal fibroblasts using RNA-seq...
2017: PloS One
https://www.readbyqxmd.com/read/28463693/natural-healing-inspired-collagen-targeting-surgical-protein-glue-for-accelerated-scarless-skin-regeneration
#8
Eun Young Jeon, Bong-Hyuk Choi, Dooyup Jung, Byeong Hee Hwang, Hyung Joon Cha
Skin scarring after deep dermal injuries is a major clinical problem due to the current therapies limited to established scars with poor understanding of healing mechanisms. From investigation of aberrations within the extracellular matrix involved in pathophysiologic scarring, it was revealed that one of the main factors responsible for impaired healing is abnormal collagen reorganization. Here, inspired by the fundamental roles of decorin, a collagen-targeting proteoglycan, in collagen remodeling, we created a scar-preventive collagen-targeting glue consisting of a newly designed collagen-binding mussel adhesive protein and a specific glycosaminoglycan...
July 2017: Biomaterials
https://www.readbyqxmd.com/read/28463592/salmon-nasal-cartilage-proteoglycan-enhances-growth-of-normal-human-dermal-fibroblast-through-erk1-2-phosphorylation
#9
Masahiro Sano, Yi Shang, Akio Nakane, Tomoaki Saito
Proteoglycan (PG) is a heavily glycosylated protein, localized to cell surface and extracellular matrix, and has various functions. Recently, it has been gradually revealed that PG interacts with various growth factors and morphogens and regulates cellular functions. Although salmon nasal cartilage PG (Salmon-PG) increases proliferation of immortalized cells, its mechanism remains unclear. In this study, we confirmed the effect of Salmon-PG on normal human dermal fibroblast (NHDF) and investigated the mechanism of PG action on NHDF...
May 2, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28461747/combining-nano-physical-and-computational-investigations-to-understand-the-nature-of-aging-in-dermal-collagen
#10
Tarek Ahmed, Anthony Nash, Kristina En Clark, Marion Ghibaudo, Nora H de Leeuw, Anne Potter, Richard Stratton, Helen L Birch, Ramona Enea Casse, Laurent Bozec
The extracellular matrix of the dermis is a complex, dynamic system with the various dermal components undergoing individual physiologic changes as we age. Age-related changes in the physical properties of collagen were investigated in particular by measuring the effect of aging, most likely due to the accumulation of advanced glycation end product (AGE) cross-links, on the nanomechanical properties of the collagen fibril using atomic force microscope nano-indentation. An age-related decrease in the Young's modulus of the transverse fibril was observed (from 8...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28448819/construction-of-three-dimensional-vascularized-functional-human-liver-tissue-using-a-layer-by-layer-cell-coating-technique
#11
Kazuki Sasaki, Takami Akagi, Tadafumi Asaoka, Hidetoshi Eguchi, Yasunari Fukuda, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Hiroshi Wada, Kunihito Gotoh, Koichi Kawamoto, Yuichiro Doki, Masaki Mori, Mitsuru Akashi
The creation of artificial liver tissue is an active area of research due to the shortage of donors for liver transplantation. Here we investigated whether a simple and efficient cell coating technique developed in our laboratory could be used to generate functional vascularized liver tissue. This technique creates three-dimensional tissue by loading cells sterically onto other cells that have been coated with layer-by-layer (LbL) nanofilms of fibronectin and gelatin, two extracellular matrix proteins. We used this technique to construct homogenous, dense, well-vascularized liver tissue from cryopreserved human primary hepatocytes, human umbilical vein endothelial cells, and normal human dermal fibroblasts...
February 28, 2017: Biomaterials
https://www.readbyqxmd.com/read/28448484/tissue-engineered-3d-human-lymphatic-microvascular-network-for-in-vitro-studies-of-lymphangiogenesis
#12
Laure Gibot, Todd Galbraith, Jennifer Bourland, Anita Rogic, Mihaela Skobe, François A Auger
This protocol describes a unique in vitro method for the generation of a 3D human lymphatic network within native connective tissue devoid of any exogenous material such as scaffolds or growth factors. In this five-stage protocol, human lymphatic endothelial cells (LECs) cocultured with dermal fibroblasts spontaneously organize into a stable 3D lymphatic capillary network. Stage 1 involves the isolation of primary fibroblasts and LECs from human skin. Fibroblasts are then cultured to produce connective tissue rich in extracellular matrix (stage 2), onto which LECs are seeded to form a network (stage 3)...
May 2017: Nature Protocols
https://www.readbyqxmd.com/read/28432900/lysophosphatidic-acid-induces-expression-of-genes-in-human-oral-keratinocytes-involved-in-wound-healing
#13
Hong Huynh Thorlakson, Stian Andre Engen, Olav Schreurs, Karl Schenck, Inger Johanne Schytte Blix
OBJECTIVE: Epithelial cells participate in wound healing by covering wounds, but also as important mediators of wound healing processes. Topical application of the phospholipid growth factor lysophosphatidic acid (LPA) accelerates dermal wound healing and we hypothesized that LPA can play a role in human oral wound healing through its effects on human oral keratinocytes (HOK). DESIGN: HOK were isolated from gingival biopsies and exposed to LPA. The LPA receptor profile, signal transduction pathways, gene expression and secretion of selected cytokines were analyzed...
April 13, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28422173/critical-involvement-of-zeb2-in-collagen-fibrillogenesis-the-molecular-similarity-between-mowat-wilson-syndrome-and-ehlers-danlos-syndrome
#14
Mika Teraishi, Mikiro Takaishi, Kimiko Nakajima, Mitsunori Ikeda, Yujiro Higashi, Shinji Shimoda, Yoshinobu Asada, Atsushi Hijikata, Osamu Ohara, Yoko Hiraki, Seiji Mizuno, Toshiyuki Fukada, Takahisa Furukawa, Nobuaki Wakamatsu, Shigetoshi Sano
Mowat-Wilson syndrome (MOWS) is a congenital disease caused by de novo heterozygous loss of function mutations or deletions of the ZEB2 gene. MOWS patients show multiple anomalies including intellectual disability, a distinctive facial appearance, microcephaly, congenital heart defects and Hirschsprung disease. However, the skin manifestation(s) of patients with MOWS has not been documented in detail. Here, we recognized that MOWS patients exhibit many Ehlers-Danlos syndrome (EDS)-like symptoms, such as skin hyperextensibility, atrophic scars and joint hypermobility...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28418608/methods-for-the-isolation-and-3d-culture-of-dermal-papilla-cells-from-human-hair-follicles
#15
H Topouzi, N J Logan, G Williams, C A Higgins
The dermal papilla is a cluster of mesenchymal cells located at the base of the hair follicle which have a number of important roles in the regulation of hair growth. As a consequence, in vitro models of these cells are widely used to study the molecular mechanisms which underlie hair follicle induction, growth and maintenance. While dermal papilla from rodent hair follicles can be digested prior to cell isolation, the unique extracellular matrix composition found in human dermal papilla renders enzymes such as trypsin and collagenase insufficient for digestion of the dermal papilla into a single cell suspension...
April 18, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28370945/alternatively-activated-macrophages-derived-from-thp-1-cells-promote-the-fibrogenic-activities-of-human-dermal-fibroblasts
#16
Zhensen Zhu, Jie Ding, Zengshuan Ma, Takashi Iwashina, Edward E Tredget
Macrophages play a key role in the wound healing process and can be divided into classically activated macrophages (M1) and alternatively activated macrophages (M2). Fibroblasts maintain the physical integrity of connective tissue, participate in wound closure as well as produce and remodel extracellular matrix. Macrophages have a close relationship with fibroblasts by increasing the production of matrix metalloproteinase-1 (MMP-1) for faster wound closure and remodeling and myofibroblast differentiation from fibroblasts...
April 3, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28370679/secreted-factors-from-equine-mesenchymal-stromal-cells-diminish-the-effects-of-tgf-%C3%AE-1-on-equine-dermal-fibroblasts-and-alter-the-phenotype-of-dermal-fibroblasts-isolated-from-cutaneous-fibroproliferative-wounds
#17
Rebecca M Harman, Ivanna V Bihun, Gerlinde R Van de Walle
The prevalence of cutaneous fibroproliferative disorders (CFPDs) is high and almost exclusively occurs in humans (keloids and hypertrophic scars) and horses (exuberant granulation tissue), making the horse a valuable translational model for studies on prevention and treatment of human CFPDs. CFPDs arise as a result of dysregulated wound healing characterized by persistently high levels of cytokines, such as transforming growth factor beta 1 (TGF-β1), that contribute to excessive extracellular matrix deposition, and the physical disorganization of dermal fibroblasts (DF)...
April 1, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28370536/the-impact-of-transcriptional-factor-fli1-deficiency-on-the-regulation-of-angiogenesis
#18
Tetsuo Toyama, Yoshihide Asano, Takuya Miyagawa, Kouki Nakamura, Megumi Hirabayashi, Takashi Yamashita, Ryosuke Saigusa, Shunsuke Miura, Yohei Ichimura, Takehiro Takahashi, Takashi Taniguchi, Ayumi Yoshizaki, Shinichi Sato
The insufficiency of Friend leukemia virus integration 1 (Fli1), a member of the Ets family transcription factors, is implicated in the pathogenesis of vasculopathy associated with systemic sclerosis (SSc). Fli1 deficiency accelerates early steps of angiogenesis, including detachment of pre-existing pericytes and extracellular matrix degradation by endothelial proteinases, but the impact of Fli1 deficiency on the other steps of angiogenesis has not been investigated. Therefore, we evaluated the effect of Fli1 deficiency on migration, proliferation, cell survival, and tube formation of human dermal microvascular endothelial cells (HDMECs)...
March 31, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28363295/biological-principles-of-scar-and-contracture
#19
REVIEW
Peter O Kwan, Edward E Tredget
Hypertrophic scar and contracture in burn patients is a complex process. Contributing factors include critical injury depth and activation of key cell subpopulations, including deep dermal fibroblasts, myofibroblasts, fibrocytes, and T-helper cells, which cause scarring rather than regeneration. These cells influence each other via cellular profibrotic and antifibrotic signals, which help to determine the outcome. These cells also both modify and interact with extracellular matrix of the wound, ultimately forming hypertrophic scar...
May 2017: Hand Clinics
https://www.readbyqxmd.com/read/28356446/fibroblast-specific-genetic-manipulation-of-p38-mapk-in-vivo-reveals-its-central-regulatory-role-in-fibrosis
#20
Jeffery D Molkentin, Darrian Bugg, Natasha Ghearing, Lisa E Dorn, Peter Kim, Michelle A Sargent, Jagadambika Gunaje, Kinya Otsu, Jennifer M Davis
Background -In the heart acute injury induces a fibrotic healing response that generates collagen rich scarring that is at first protective but if inappropriately sustained can worsen heart disease. The fibrotic process is initiated by cytokines, neuroendocrine effectors and mechanical strain that promote resident fibroblast differentiation into contractile and extracellular matrix producing myofibroblasts. The mitogen-activated protein kinase (MAPK) p38α (Mapk14 gene) is known to influence the cardiac injury response, but its direct role in orchestrating programmed fibroblast differentiation and fibrosis in vivo is unknown...
March 29, 2017: Circulation
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