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https://www.readbyqxmd.com/read/28647243/quantification-of-hookworm-ova-from-wastewater-matrices-using-quantitative-pcr
#1
Pradip Gyawali, Warish Ahmed, Jatinder P Sidhu, Paul Jagals, Simon Toze
A quantitative PCR (qPCR) assay was used to quantify Ancylostoma caninum ova in wastewater and sludge samples. We estimated the average gene copy numbers for a single ovum using a mixed population of ova. The average gene copy numbers derived from the mixed population were used to estimate numbers of hookworm ova in A. caninum seeded and unseeded wastewater and sludge samples. The newly developed qPCR assay estimated an average of 3.7×10(3) gene copies per ovum, which was then validated by seeding known numbers of hookworm ova into treated wastewater...
July 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28633651/epidemiology-of-intestinal-helminthiasis-among-school-children-with-emphasis-on-schistosoma-mansoni-infection-in-wolaita-zone-southern-ethiopia
#2
Bereket Alemayehu, Zewdneh Tomass, Fiseha Wadilo, Dawit Leja, Song Liang, Berhanu Erko
BACKGROUND: Intestinal helminth infections are major parasitic diseases causing public health problems in Ethiopia. Although the epidemiology of these infections are well documented in Ethiopia, new transmission foci for schistosomiasis are being reported in different parts of the country. The objective of this study was to assess the prevalence of Schistosoma mansoni and other intestinal helminth infections among school children and determine the endemicity of schistosomiasis in Wolaita Zone, southern Ethiopia...
June 20, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28632749/using-a-health-rating-system-to-evaluate-the-usefulness-of-caenorhabditis-elegans-as-a-model-for-anthelmintic-study
#3
Kathryn J Weaver, Cassandra J May, Brian L Ellis
Soil-transmitted helminths (STHs) are intestinal parasitic nematodes that infect humans, and are transmitted through contaminated soil. These nematodes include the large roundworm (Ascaris lumbricoides), whipworm (Trichuris trichiura), and hookworm (Ancylostoma ceylanicum, Ancylostoma duodenale, and Necator americanus). Nearly 1.5 billion people (~24% of the population) worldwide are infected with at least one species of these parasites, burdening the poor, in particular, children and pregnant women. To combat these diseases, the WHO only recognizes four anthelmintic drugs, including the preferred drug, albendazole, for mass drug administration (MDA)...
2017: PloS One
https://www.readbyqxmd.com/read/28624508/epidemiological-profile-of-cutaneous-larva-migrans-in-travelers-returning-to-france-between-2003-and-2015
#4
Doudou Sow, Ferelaha Soro, Emilie Javelle, Fabrice Simon, Philippe Parola, Philippe Gautret
BACKGROUND: Cutaneous larva migrans (CLM) is caused by the penetration and migration of canine or feline hookworm larvae into the skin, and frequently affects travelers from high-income countries returning from tropical and sub-tropical areas. Unfortunately, this pathology is neglected due to poor understanding among both physicians and travelers. This study describes the profile of cases reported in Marseille, France. PATIENTS AND METHODS: A retrospective study was performed in two specialized hospitals between 2003 and 2015...
June 15, 2017: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/28619671/combination-of-five-diagnostic-tests-to-estimate-the-prevalence-of-hookworm-infection-among-school-aged-children-from-a-rural-area-of-colombia
#5
Rafael E Barreto, Javier Narváez, Natalia A Sepúlveda, Fabián C Velásquez, Sandra C Díaz, Myriam Consuelo López, Patricia Reyes, Ligia I Moncada
BACKGROUND: Public health programs for the control of soil-transmitted helminthiases require valid diagnostic tests for surveillance and parasitic control evaluation. However, there is currently no agreement about what test should be used as a gold standard for the diagnosis of hookworm infection. Still, in presence of concurrent data for multiple tests it is possible to use statistical models to estimate measures of test performance and prevalence. The aim of this study was to estimate the diagnostic accuracy of five parallel tests (direct microscopic examination, Kato-Katz, Harada-Mori, modified Ritchie-Frick, and culture in agar plate) to detect hookworm infections in a sample of school-aged children from a rural area in Colombia...
June 12, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28617281/comparison-of-culture-based-vital-stain-and-pma-qpcr-methods-for-the-quantitative-detection-of-viable-hookworm-ova
#6
P Gyawali, J P S Sidhu, W Ahmed, P Jagals, S Toze
Accurate quantitative measurement of viable hookworm ova from environmental samples is the key to controlling hookworm re-infections in the endemic regions. In this study, the accuracy of three quantitative detection methods [culture-based, vital stain and propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR)] was evaluated by enumerating 1,000 ± 50 Ancylostoma caninum ova in the laboratory. The culture-based method was able to quantify an average of 397 ± 59 viable hookworm ova. Similarly, vital stain and PMA-qPCR methods quantified 644 ± 87 and 587 ± 91 viable ova, respectively...
June 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28616506/the-hookworm-ancylostoma-ceylanicum-an-emerging-public-health-risk-in-australian-tropical-rainforests-and-indigenous-communities
#7
Felicity A Smout, Lee F Skerratt, James R A Butler, Christopher N Johnson, Bradley C Congdon, R C Andrew Thompson
Ancylostoma ceylanicum is the common hookworm of domestic dogs and cats throughout Asia, and is an emerging but little understood public health risk in tropical northern Australia. We investigated the prevalence of A. ceylanicum in soil and free-ranging domestic dogs at six rainforest locations in Far North Queensland that are Indigenous Australian communities and popular tourist attractions within the Wet Tropics World Heritage Area. By combining PCR-based techniques with traditional methods of hookworm species identification, we found the prevalence of hookworm in Indigenous community dogs was high (96...
June 2017: One Health
https://www.readbyqxmd.com/read/28615871/morphometric-and-scanning-electron-microscopy-based-identification-of-ancylostoma-caninum-parasites-in-dog
#8
Harsimarandeep Singh Uppal, Mandeep Singh Bal, L D Singla, Paramjit Kaur, B S Sandhu
Ancylostoma caninum, a blood feeding nematode parasite (Family: Ancylostomatidae, Superfamily: Ancylostomatoidea) can cause anaemia, dark reddish-brown to black haemorrhagic diarrhoea, dehydration, wasting and deaths due to heavy blood loss. Adult hook worm parasites recovered from the intestine of a stray dog at the time of necropsy were identified as A. caninum based on morphological characters and morphometric observations involving scanning electron microscopy (SEM). Different developmental stages of hookworm eggs viz...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28615087/association-between-intestinal-helminth-infections-and-mid-upper-arm-circumference-among-children-in-sri-lanka-a-cross-sectional-study
#9
L S Galgamuwa, D Iddawela, S D Dharmaratne
Intestinal helminth infections constitute a major health problem in low socio-economic communities worldwide. A cross-sectional study was conducted in children aged between 12 and 60 months. A structured questionnaire was used to collect socio-demographic data and stool samples were subjected to direct smear, stained with Lugol's iodine, and formaldehyde-ether concentration techniques to determine the prevalence of intestinal helminth infections. The circumference of each child's left mid upper arm (MUAC) was measured using standard procedures...
June 15, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/28610937/hookworm-infections-among-migrant-workers-in-malaysia-molecular-identification-of-necator-americanus-and-ancylostoma-duodenale
#10
Norhidayu Sahimin, Yvonne Ai Lian Lim, Benacer Douadi, Mohd Khairul Nizam Mohd Khalid, John-James Wilson, Jerzy M Behnke, Siti Nursheena Mohd Zain
Ongoing urbanisation of the working population as well as cross-border migration of workers particularly into large cities has contributed to the development and growth of urban slums. These deprived areas are conducive for the transmission of intestinal pathogens including hookworm. The aim of this study was to determine both the prevalence and species identity of hookworm infections among the migrant worker community in Malaysia. A total of 388 faecal samples were collected from migrant workers between September 2014 and August 2015, representing workers from five employment sectors: construction, manufacturing, agriculture and plantations, food services and domestic services...
June 10, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28606323/coprodiagnosis-of-echinococcus-granulosus-infection-in-dogs-from-ankara-turkey
#11
Hatice Öge, Semih Öge, Bahadır Gönenç, Oğuz Sarımehmetoğlu, Gökben Özbakış
The present study were undertaken to compare two isolation techniques (centrifugal flotation and sedimentation) for recovering taeniid eggs from faecal samples, to identify E. granulosus DNA from taeniid eggs by PCR, and to determine the prevalence of E. granulosus in dogs in villages. Faecal samples were collected from 100 dogs in Ankara province. Taenia spp. eggs were found in 27% of dogs faeces. Echinococcus granulosus-specific PCR was obtained in 14 (51.85%) of the taeniid eggs-positive samples. As well as finding Taenia eggs in dogs' faeces, we also found eggs of some helminthic parasites; such as Dipylidium caninum, Toxocara canis, Toxascaris leonina, Trichuris sp...
August 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28599671/is-there-a-difference-in-prevalence-of-helminths-between-households-using-ecological-sanitation-and-those-using-traditional-pit-latrines-a-latrine-based-cross-sectional-comparative-study-in-malawi
#12
Save Kumwenda, Chisomo Msefula, Wilfred Kadewa, Yohane Diness, Charles Kato, Tracy Morse, Bagrey Ngwira
BACKGROUND: Studies have shown that households using sludge from human excreta for agriculture are at an increased risk of soil transmitted helminths. However, while use of ecological sanitation (EcoSan) latrines is increasing in most African countries including Malawi, few studies have been done to check whether use of such sludge could potentially increase the prevalence of helminthic infections among household members as a results of exposure to faecal sludge/compared to use of traditional latrines...
June 9, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28599633/prevalence-of-intestinal-parasitic-infection-and-associated-risk-factors-among-village-health-volunteers-in-rural-communities-of-southern-thailand
#13
Chuchard Punsawad, Nonthapan Phasuk, Suchirat Bunratsami, Kanjana Thongtup, Niramon Siripakonuaong, Somchok Nongnaul
BACKGROUND: Intestinal parasitic infections remain prevalent and constitute a public health problem in certain rural areas of Thailand. Village health volunteers (VHVs), who are members of a Thai healthcare alliance, function as key providers of health prevention measures, disease control, and health education and share national health promotion campaigns with community members. This study is aimed at evaluating the prevalence, intensity, and risk factors for intestinal parasitic infection in VHVs in order to design community awareness and health education campaigns for the target population...
June 9, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28576124/ancylostoma-ailuropodae-n-sp-nematoda-ancylostomatidae-a-new-hookworm-parasite-isolated-from-wild-giant-pandas-in-southwest-china
#14
Yue Xie, Eric P Hoberg, Zijiang Yang, Joseph F Urban, Guangyou Yang
BACKGROUND: Hookworms belonging to the genus Ancylostoma (Dubini, 1843) cause ancylostomiasis, a disease of considerable concern in humans and domestic and wild animals. Molecular and epidemiological data support evidence for the zoonotic potential among species of Ancylostoma where transmission to humans is facilitated by rapid urbanization and increased human-wildlife interactions. It is important to assess and describe these potential zoonotic parasite species in wildlife, especially in hosts that have physiological similarities to humans and share their habitat...
June 2, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28571565/c-reactive-protein-is-differentially-modulated-by-co-existing-infections-vitamin-deficiencies-and-maternal-factors-in-pregnant-and-lactating-indigenous-panamanian-women
#15
Doris González-Fernández, Emérita Del Carmen Pons, Delfina Rueda, Odalis Teresa Sinisterra, Enrique Murillo, Marilyn E Scott, Kristine G Koski
BACKGROUND: The usefulness of C-reactive protein (CRP) as a non-specific marker of inflammation during pregnancy and lactation is unclear in impoverished populations where co-existing infections and vitamin deficiencies are common. METHODS: This cross-sectional study in Panama recruited 120 pregnant and 99 lactating Ngäbe-Buglé women from 14 communities in rural Panama. Obstetric history, indoor wood smoke exposure, fieldwork, BMI, vitamins A, B12, D, and folic acid, and inflammation markers (CRP, neutrophil/lymphocyte ratio (NLR), plateletcrit and cytokines) were measured...
June 2, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28543307/a-bayesian-approach-to-model-the-conditional-correlation-between-several-diagnostic-tests-and-various-replicated-subjects-measurements
#16
Hélio Doyle Pereira da Silva, Carlos Ascaso, Alessandra Queiroga Gonçalves, Patricia Puccinelli Orlandi, Rosa Abellana
Two key aims of diagnostic research are to accurately and precisely estimate disease prevalence and test sensitivity and specificity. Latent class models have been proposed that consider the correlation between subject measures determined by different tests in order to diagnose diseases for which gold standard tests are not available. In some clinical studies, several measures of the same subject are made with the same test under the same conditions (replicated measurements), and thus, replicated measurements for each subject are not independent...
May 21, 2017: Statistics in Medicine
https://www.readbyqxmd.com/read/28542260/hookworm-infection-is-associated-with-decreased-cd4-t-cell-counts-in-hiv-infected-adult-ugandans
#17
Bozena M Morawski, Miya Yunus, Emmanuel Kerukadho, Grace Turyasingura, Logose Barbra, Andrew Mijumbi Ojok, Andrew R DiNardo, Stefanie Sowinski, David R Boulware, Rojelio Mejia
Most studies evaluating epidemiologic relationships between helminths and HIV have been conducted in the pre-ART era, and evidence of the impact of helminth infections on HIV disease progression remains conflicting. Less is known about helminth infection and clinical outcomes in HIV-infected adults receiving antiretroviral therapy (ART). We sampled HIV-infected adults for eight gastrointestinal parasites and correlated parasitic infection with demographic predictors, and clinical and immunologic outcomes. Contrasting with previous studies, we measured parasitic infection with a quantitative, highly sensitive and specific polymerase chain reaction (PCR) method...
May 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28535806/the-past-matters-estimating-intrinsic-hookworm-transmission-intensity-in-areas-with-past-mass-drug-administration-to-control-lymphatic-filariasis
#18
Marleen Werkman, James E Truscott, Jaspreet Toor, James E Wright, Roy M Anderson
BACKGROUND: Current WHO guidelines for soil-transmitted helminth (STH) control focus on mass drug administration (MDA) targeting preschool-aged (pre-SAC) and school-aged children (SAC), with the goal of eliminating STH as a public health problem amongst children. Recently, attention and funding has turned towards the question whether MDA alone can result in the interruption of transmission for STH. The lymphatic filariasis (LF) elimination programme, have been successful in reaching whole communities...
May 23, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28535750/prevalence-of-intestinal-parasitic-infections-and-associated-risk-factors-among-students-at-dona-berber-primary-school-bahir-dar-ethiopia
#19
Tamirat Hailegebriel
BACKGROUND: Intestinal parasitic infections are still one of the major health concerns in developing countries. Monitoring of intestinal parasitic infection and associated risk factors are essential for intervention strategies. Therefore, the aim of this study was to assess the prevalence of intestinal parasitic infection and associated risk factors among students at Dona Berber primary school, Bahir Dar, Ethiopia. METHODS: School based cross-sectional study was conducted among students at Dona Berber primary school from October 2015 to June 2016...
May 23, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28506307/intestinal-parasitic-infections-and-its-association-with-undernutrition-and-cd4-t-cell-levels-among-hiv-aids-patients-on-haart-in-butajira-ethiopia
#20
Dereje Gedle, Gemechu Kumera, Tewodros Eshete, Kasahun Ketema, Haweni Adugna, Fetuma Feyera
BACKGROUND: Intestinal parasitic infections and HIV/AIDS have been the major public health problems and remain a vital cause of morbidity and mortality in developing countries. Both problems are linked in a vicious cycle. The magnitude of intestinal parasites was prevalent among people living with HIV/AIDS even in the HAART era. However, the pertinent risk factors associated with intestinal parasites among HIV/AIDS patients were not well investigated in Ethiopia particularly at Butajira town...
May 15, 2017: Journal of Health, Population, and Nutrition
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