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https://www.readbyqxmd.com/read/28449429/pancreatic-lymphoepithelial-cysts-diagnosed-with-endosonography-guided-fine-needle-aspiration
#1
Youngmin Oh, Yonghyeok Choi, Seung Myoung Son, Jisun Lee, Yook Kim, Joung Ho Han, Seon Mee Park
Although lymphoepithelial cysts (LECs) of the pancreas are benign lesions, most of them have been treated with surgical resection due to diagnostic difficulty. We report a 66-year-old woman diagnosed with pancreatic LECs. Abdominal ultrasound revealed two masses in the pancreas, which were not visible on the abdominal computed tomography. In an abdominal magnetic resonance imaging, pancreas lesions showed solid tumors, which revealed a low signal intensity on T1-, moderate high signal intensity on T2 weighted images, and homogeneous delayed enhancement in the portal venous phase...
April 25, 2017: Korean Journal of Gastroenterology, Taehan Sohwagi Hakhoe Chi
https://www.readbyqxmd.com/read/28448054/lipid-droplet-isolation-for-quantitative-mass-spectrometry-analysis
#2
Kathrin Rösch, Marcel Kwiatkowski, Hartmut Schlüter, Eva Herker
Lipid droplets are vital to the replication of a variety of different pathogens, most prominently the Hepatitis C Virus (HCV), as the putative site of virion morphogenesis. Quantitative lipid droplet proteome analysis can be used to identify proteins that localize to or are displaced from lipid droplets under conditions such as virus infections. Here, we describe a protocol that has been successfully used to characterize the changes in the lipid droplet proteome following infection with HCV. We use Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC) and thus label the complete proteome of one population of cells with "heavy" amino acids to quantitate the proteins by mass spectrometry...
April 17, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28446968/the-role-of-transforming-growth-factor-tgf-%C3%AE-in-the-infarcted-myocardium
#3
REVIEW
Nikolaos G Frangogiannis
The adult mammalian heart has negligible regenerative capacity. Following myocardial infarction, sudden necrosis of cardiomyocytes triggers an intense inflammatory reaction that clears the wound from dead cells and matrix debris, while activating a reparative program. A growing body of evidence suggests that members of the transforming growth factor (TGF)-β family critically regulate the inflammatory and reparative response following infarction. Although all three TGF-β isoforms (TGF-β1, -β2 and -β3) are markedly upregulated in the infarcted myocardium, information on isoform-specific actions is limited...
March 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28446444/concurrent-isolation-of-three-distinct-cardiac-stem-cell-populations-from-a-single-human-heart-biopsy
#4
Megan M Monsanto, Kevin S White, Taeyong Kim, Bingyan J Wang, Kristina Fisher, Kelli Ilves, Farid G Khalafalla, Alexandria Casillas, Kathleen Broughton, Sadia Mohsin, Walter P Dembitsky, Mark A Sussman
Rationale: The relative actions and synergism between distinct myocardial-derived stem cell populations remains obscure. Ongoing debates regarding optimal cell population(s) for treatment of heart failure prompted implementation of a protocol for isolation of multiple stem cell populations from a single myocardial tissue sample to develop new insights for achieving myocardial regeneration. Objective: Establish a robust cardiac stem cell isolation and culture protocol to consistently generate three distinct stem cell populations from a single human heart biopsy...
April 26, 2017: Circulation Research
https://www.readbyqxmd.com/read/28441569/the-cytoskeleton-autophagy-connection
#5
REVIEW
David J Kast, Roberto Dominguez
Actin cytoskeleton dynamics play vital roles in most forms of intracellular trafficking by promoting the biogenesis and transport of vesicular cargoes. Mounting evidence indicates that actin dynamics and membrane-cytoskeleton scaffolds also have essential roles in macroautophagy, the process by which cellular waste is isolated inside specialized vesicles called autophagosomes for recycling and degradation. Branched actin polymerization is necessary for the biogenesis of autophagosomes from the endoplasmic reticulum (ER) membrane...
April 24, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28440943/microplastics-as-a-vector-of-hydrophobic-contaminants-importance-of-hydrophobic-additives
#6
Jung-Hwan Kwon, Sein Chang, Sang Hee Hong, Won Joon Shim
Despite a recent boom in research on the environmental fate, distribution, and harmful effects of chemical substances associated with marine plastic debris, no consensus has been reached on whether chemicals originating from microplastics cause serious environmental harm. For the risk assessment of chemical contaminants associated with microplastics, it would be useful to group organic chemicals into 2 categories: additives and nonadditives. Whereas plastic particles are not likely to be diffuse sources of chemicals that are not intentionally added to plastic products, continuous leaching of additives would result in higher concentrations, at least at a local scale...
May 2017: Integrated Environmental Assessment and Management
https://www.readbyqxmd.com/read/28440926/sources-and-dispersive-modes-of-micro-fibers-in-the-environment
#7
Steve A Carr
Understanding the sources and distribution of microfibers (MFs) in the environment is critical if control and remediation measures are to be effective. Microfibers comprise an overwhelming fraction (>85%) of microplastic debris found on shorelines around the world. Although primary sources have not been fully vetted, until recently it was widely believed that domestic laundry discharges were the major source. It was also thought that synthetic fibers and particles having dimensions <5 mm easily bypassed filtration and other solid separation processes at wastewater treatment plants (WWTPs) and entered oceans and surface waters...
May 2017: Integrated Environmental Assessment and Management
https://www.readbyqxmd.com/read/28440925/microplastics-in-the-context-of-regulation-of-commercial-shellfish-aquaculture-operations
#8
Rosalind A Schoof, Jesse DeNike
Shellfish aquaculture in the Salish Sea (encompassing the Strait of Juan de Fuca, Puget Sound, and the Georgia Strait) is a major source of clams, oysters, and mussels in the United States and Canada. Plastic gear is necessary for the viability of many of these operations. During the past few years, shellfish farm permits issued in Washington State have been challenged on various bases that have included allegations that the plastic gear is releasing microplastics, commonly defined as particles less than 5 mm in diameter...
May 2017: Integrated Environmental Assessment and Management
https://www.readbyqxmd.com/read/28440924/current-understanding-of-microplastics-in-the-environment-occurrence-fate-risks-and-what-we-should-do
#9
Jinping Peng, Jundong Wang, Liqi Cai
Microplastics pollution has been documented in the global environment, including at sea, in freshwater and in atmospheric fallout. Ingestion of microplastics by multiple kinds of organisms has been reported and has received increasing attention, because microplastics not only act as a source of toxic chemicals but also a sink for toxic chemicals. To better understand the great concerns about microplastics and associated toxic chemicals potential exposed to the organisms ingesting the debris, we should know more about the occurrence, fate, and risks of microplastics in the environment...
May 2017: Integrated Environmental Assessment and Management
https://www.readbyqxmd.com/read/28439897/platelet-lysate-obtained-via-plateletpheresis-performed-in-standing-and-awake-equine-donors
#10
Scarlett M Sumner, Maria C Naskou, Merrilee Thoresen, Ian Copland, John F Peroni
BACKGROUND: Platelet preparations containing growth factors, attachment factors, and enzymes are appealing to enhance healing of injured tissues and as an alternative to xenogenic serum in cell culture media. Plateletpheresis is commonly used to collect platelets in human medicine but has not been validated in horses. STUDY DESIGN AND METHODS: Plateletpheresis to collect platelet concentrate was performed on six female, mixed breed, chemically restrained horses using commercially available apheresis equipment...
April 25, 2017: Transfusion
https://www.readbyqxmd.com/read/28439534/the-arctic-ocean-as-a-dead-end-for-floating-plastics-in-the-north-atlantic-branch-of-the-thermohaline-circulation
#11
Andrés Cózar, Elisa Martí, Carlos M Duarte, Juan García-de-Lomas, Erik van Sebille, Thomas J Ballatore, Victor M Eguíluz, J Ignacio González-Gordillo, Maria L Pedrotti, Fidel Echevarría, Romain Troublè, Xabier Irigoien
The subtropical ocean gyres are recognized as great marine accummulation zones of floating plastic debris; however, the possibility of plastic accumulation at polar latitudes has been overlooked because of the lack of nearby pollution sources. In the present study, the Arctic Ocean was extensively sampled for floating plastic debris from the Tara Oceans circumpolar expedition. Although plastic debris was scarce or absent in most of the Arctic waters, it reached high concentrations (hundreds of thousands of pieces per square kilometer) in the northernmost and easternmost areas of the Greenland and Barents seas...
April 2017: Science Advances
https://www.readbyqxmd.com/read/28438895/the-casper-carotid-artery-stent-a-unique-all-metal-micromesh-stent-designed-to-prevent-embolic-release
#12
Orlando Diaz, Gloria Lopez, John O F Roehm, Ginna De la Rosa, Fernando Orozco, Rafael Almeida
BACKGROUND: Stroke due to the release of embolic debris during the placement of a stent to correct carotid artery stenosis is a constant procedural and peri-procedural threat. The new all metal Casper stent has been created with two layers of nitinol, the inner layer of which has pores diminutive enough to prevent embolic release. OBJECTIVE: To evaluate the safety, effectiveness, and utility of the double layer nitinol Casper carotid artery stent in the treatment of patients with severe carotid artery stenosis...
April 24, 2017: Journal of Neurointerventional Surgery
https://www.readbyqxmd.com/read/28437194/comparison-of-three-final-irrigation-activation-techniques-effects-on-canal-cleanness-smear-layer-removal-and-dentinal-tubule-penetration-of-two-root-canal-sealers
#13
Elmas Turkel, Emel Olga Onay, Mete Ungor
OBJECTIVE: The aim of this study was to compare three final irrigation activation techniques with respect to their effects on debridement efficacy, smear layer removal, and dentinal tubule penetration of two different root canal sealers. BACKGROUND DATA: Different applications to improve the delivery of irrigating solutions within the root canal system are currently being investigated, as not all of the mechanisms and effects of these techniques have been clearly identified...
February 22, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28436148/relationship-between-self-assessment-and-clinical-evaluation-of-dental-plaque-and-gingival-condition-in-japanese-adolescents
#14
Y Shizuma, T Zaitsu, M Ueno, M Ohnuki, Y Kawaguchi
OBJECTIVES: To apply a self-administered assessment form about dental plaque level and gingival condition to Japanese adolescents and to examine the extent to which they can evaluate their own dental plaque and gingivae by comparing with dentists' clinical evaluation. METHODS: Participants were 151 senior high school students (adolescents) who observed their own mouths and recorded dental plaque seen on their 12 anterior teeth, and gingival inflammation condition of 10 anterior interdental papillae, on a self-assessment form...
April 23, 2017: International Journal of Dental Hygiene
https://www.readbyqxmd.com/read/28434664/marine-debris-in-beaches-of-the-southwestern-atlantic-an-assessment-of-their-abundance-and-mass-at-different-spatial-scales-in-northern-coastal-argentina
#15
Maria Eugenia Becherucci, Alan Federico Rosenthal, Juan Pablo Seco Pon
Argentina is currently undergoing an intensive development of coastal-oriented tourism due to the temperate climate and coastal sceneries of the Southwestern Atlantic and particularly its wide ocean-open sandy beaches, which may turn into an important contributor of marine debris to the beaches. This study was designed to assess at four spatial scales (i) the variation of the abundance and mass of marine debris and (ii) the composition and sources of these items in sandy-tourist beaches of coastal zones of the province of Buenos Aires, in northern Argentina...
April 20, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28431744/incidence-of-marine-debris-in-seabirds-feeding-at-different-water-depths
#16
D C Tavares, J F de Moura, A Merico, S Siciliano
Marine debris such as plastic fragments and fishing gears are accumulating in the ocean at alarming rates. This study assesses the incidence of debris in the gastrointestinal tracts of seabirds feeding at different depths and found stranded along the Brazilian coast in the period 2010-2013. More than half (55%) of the species analysed, corresponding to 16% of the total number of individuals, presented plastic particles in their gastrointestinal tracts. The incidence of debris was higher in birds feeding predominantly at intermediate (3-6m) and deep (20-100m) waters than those feeding at surface (<2m)...
April 18, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28431414/intratracheal-instillation-of-neutrophils-rescues-bacterial-overgrowth-initiated-by-trauma-damage-associated-molecular-patterns
#17
Kiyoshi Itagaki, Ingred Riça, Jing Zhang, Dave Gallo, Melissa DePrato, Leo E Otterbein, Carl J Hauser
BACKGROUND: Nosocomial pneumonias are common in trauma patients and so interventions to prevent and treat nosocomial pneumonia may improve outcomes. Our prior work strongly suggests that tissue injury predisposes to infections like nosocomial pneumonia because mitochondrial debris originating from injured cells contains damage-associated molecular patterns that can reduce neutrophil (PMN) migration into the airway and diminish PMN function in response to bacterial inoculation of the airway...
May 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28426158/a-novel-technique-for-decellularization-of-allogenic-nerves-and-in-vivo-study-of-their-use-for-peripheral-nerve-reconstruction
#18
F Boriani, N Fazio, C Fotia, L Savarino, N Nicoli Aldini, L Martini, N Zini, M Bernardini, N Baldini
Autografts represent the gold standard for peripheral nerve reconstruction but their limited availability, the discrepancy of nerve caliber and long surgical times are drawbacks. Allografts have therefore become a valid alternative option. In particular, acellular nerve allografts (ANAs) rather than fresh allografts do not need immunosuppression and appear to be safe and effective based on recent studies. An innovative method was conceived to obtain ANAs, so as to speed up nerve decellularization, without compromising nerve architecture, and without breaking the asepsis chain...
April 20, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28425503/nerve-degeneration-and-regeneration-in-the-cephalopod-mollusc-octopus-vulgaris-the-case-of-the-pallial-nerve
#19
Pamela Imperadore, Sameer B Shah, Helen P Makarenkova, Graziano Fiorito
Regeneration is a process that restores structure and function of tissues damaged by injury or disease. In mammals complete regeneration is often unsuccessful, while most of the low phyla animals can re-grow many parts of their body after amputation. Cephalopod molluscs, and in particular Octopus vulgaris, are well known for their capacity to regenerate their arms and other body parts, including central and peripheral nervous system. To better understand the mechanism of recovery following nerve injury in this species we investigated the process of axon regrowth and nerve regeneration after complete transection of the Octopus pallial nerves...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28425458/anatomy-of-terminal-moraine-segments-and-implied-lake-stability-on-ngozumpa-glacier-nepal-from-electrical-resistivity-tomography-ert
#20
Sarah S Thompson, Bernd Kulessa, Douglas I Benn, Jordan R Mertes
Moraine-dammed lakes at debris-covered glaciers are becoming increasingly common and pose significant outburst flood hazards if the dam is breached. While moraine subsurface structure and internal processes are likely to influence dam stability, only few sites have so far been investigated. We conducted electrical resistivity tomography (ERT) surveys at two sites on the terminal moraine complex of the Ngozumpa Glacier, Nepal, to aid assessment of future terminus stability. The resistivity signature of glacier ice at the site (100-15 kΩ m) is more consistent with values measured from cold glacier ice and while this may be feasible, uncertainties in the data inversion introduce ambiguity to this thermal interpretation...
April 20, 2017: Scientific Reports
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