keyword
https://read.qxmd.com/read/38475827/endophilin-a2-controls-touch-and-mechanical-allodynia-via-kinesin-mediated-piezo2-trafficking
#1
JOURNAL ARTICLE
Man-Xiu Xie, Ren-Chun Lai, Yi-Bin Xiao, Xi Zhang, Xian-Ying Cao, Xiao-Yu Tian, An-Nan Chen, Zi-Yi Chen, Yan Cao, Xiao Li, Xiao-Long Zhang
BACKGROUND: Tactile and mechanical pain are crucial to our interaction with the environment, yet the underpinning molecular mechanism is still elusive. Endophilin A2 (EndoA2) is an evolutionarily conserved protein that is documented in the endocytosis pathway. However, the role of EndoA2 in the regulation of mechanical sensitivity and its underlying mechanisms are currently unclear. METHODS: Male and female C57BL/6 mice (8-12 weeks) and male cynomolgus monkeys (7-10 years old) were used in our experiments...
March 12, 2024: Military Medical Research
https://read.qxmd.com/read/38160255/bound-ions-effects-using-machine-learning-method-to-study-the-kinesin-ncd-binding-with-microtubule
#2
JOURNAL ARTICLE
Wenhan Guo, Dan Du, Houfang Zhang, Jason E Sanchez, Shengjie Sun, Wang Xu, Yunhui Peng, Lin Li
Drosophila Ncd proteins are motor proteins that play important roles in spindle organization. The Ncd and tubulin dimer are highly charged. Thus, it is crucial to investigate Ncd-tubulin dimer interactions in the presence of ions, especially ions that are bound or restricted at the Ncd-tubulin dimer binding interfaces. To consider the ion effects, the widely used implicit solvent models treat ions implicitly in the continuous solvent environment, without focusing on the individual ions' effects. But the highly charged biomolecules such as the Ncd and tubulin dimer, may capture some ions at the highly charged regions as bound ions...
December 29, 2023: Biophysical Journal
https://read.qxmd.com/read/38124277/increases-in-anterograde-axoplasmic-transport-in-neurons-of-the-hyper-glutamatergic-glutamate-dehydrogenase-1-glud1-transgenic-mouse-effects-of-glutamate-receptors-on-transport
#3
JOURNAL ARTICLE
Phil Lee, Jieun Kim, In-Young Choi, Ranu Pal, Dongwei Hui, Joanne K Marcario, Mary L Michaelis, Elias K Michaelis
The excitatory neurotransmitter glutamate has a role in neuronal migration and process elongation in the central nervous system (CNS). The effects of chronic glutamate hyperactivity on vesicular and protein transport within CNS neurons, that is, processes necessary for neurite growth, have not been examined previously. In this study, we measured the effects of lifelong hyperactivity of glutamate neurotransmission on axoplasmic transport in CNS neurons. We compared wild-type (wt) to transgenic (Tg) mice over-expressing the glutamate dehydrogenase gene Glud1 in CNS neurons and exhibiting increases in glutamate transmitter formation, release, and synaptic activation in brain throughout the lifespan...
December 20, 2023: Journal of Neurochemistry
https://read.qxmd.com/read/37759474/presynaptic-precursor-vesicles-cargo-biogenesis-and-kinesin-based-transport-across-species
#4
REVIEW
Astrid G Petzoldt
The faithful formation and, consequently, function of a synapse requires continuous and tightly controlled delivery of synaptic material. At the presynapse, a variety of proteins with unequal molecular properties are indispensable to compose and control the molecular machinery concerting neurotransmitter release through synaptic vesicle fusion with the presynaptic membrane. As presynaptic proteins are produced mainly in the neuronal soma, they are obliged to traffic along microtubules through the axon to reach the consuming presynapse...
September 11, 2023: Cells
https://read.qxmd.com/read/36745293/multi-scale-modelling-of-shear-stress-on-the-syncytiotrophoblast-could-maternal-blood-flow-impact-placental-function-across-gestation
#5
JOURNAL ARTICLE
Tet Chuan Lee, Ali Moulvi, Joanna L James, Alys R Clark
The placenta is a critical fetal exchange organ, with a complex branching tree-like structure. Its surface is covered by a single multinucleated cell, the syncytiotrophoblast, which bathes in maternal blood for most of pregnancy. Mechanosensing protein expression by the syncytiotrophoblast at term suggests that shear stress exerted by maternal blood flow may modulate placental development and function. However, it is not known how the mechanosensitive capacity of the syncytiotrophoblast, or the shear stress it experiences, change across gestation...
February 6, 2023: Annals of Biomedical Engineering
https://read.qxmd.com/read/36637158/testing-the-ion-current-model-for-flagellar-length-sensing-and-ift-regulation
#6
JOURNAL ARTICLE
Hiroaki Ishikawa, Jeremy Moore, Dennis R Diener, Markus Delling, Wallace F Marshall
Eukaryotic cilia and flagella are microtubule-based organelles whose relatively simple shape makes them ideal for investigating the fundamental question of organelle size regulation. Most of the flagellar materials are transported from the cell body via an active transport process called intraflagellar transport (IFT). The rate of IFT entry into flagella, known as IFT injection, has been shown to negatively correlate with flagellar length. However, it remains unknown how the cell measures the length of its flagella and controls IFT injection...
January 13, 2023: ELife
https://read.qxmd.com/read/36504683/inhibition-of-glycogen-synthase-kinase-3-by-lithium-a-mechanism-in-search-of-specificity
#7
REVIEW
Dipashree Chatterjee, Jean Martin Beaulieu
Inhibition of Glycogen synthase kinase 3 (GSK3) is a popular explanation for the effects of lithium ions on mood regulation in bipolar disorder and other mental illnesses, including major depression, cyclothymia, and schizophrenia. Contribution of GSK3 is supported by evidence obtained from animal and patient derived model systems. However, the two GSK3 enzymes, GSK3α and GSK3β, have more than 100 validated substrates. They are thus central hubs for major biological functions, such as dopamine-glutamate neurotransmission, synaptic plasticity (Hebbian and homeostatic), inflammation, circadian regulation, protein synthesis, metabolism, inflammation, and mitochondrial functions...
2022: Frontiers in Molecular Neuroscience
https://read.qxmd.com/read/36304122/kinesin-2-motors-differentially-impact-biogenesis-of-extracellular-vesicle-subpopulations-shed-from-sensory-cilia
#8
JOURNAL ARTICLE
Michael Clupper, Rachael Gill, Malek Elsayyid, Denis Touroutine, Jeffrey L Caplan, Jessica E Tanis
Extracellular vesicles (EVs) are bioactive lipid-bilayer enclosed particles released from nearly all cells. One specialized site for EV shedding is the primary cilium. Here, we discover the conserved ion channel CLHM-1 as a ciliary EV cargo. Imaging of EVs released from sensory neuron cilia of Caenorhabditis elegans expressing fluorescently tagged CLHM-1 and TRP polycystin-2 channel PKD-2 shows enrichment of these cargoes in distinct EV subpopulations that are differentially shed in response to mating partner availability...
November 18, 2022: IScience
https://read.qxmd.com/read/36291554/common-patterns-of-hydrolysis-initiation-in-p-loop-fold-nucleoside-triphosphatases
#9
JOURNAL ARTICLE
Maria I Kozlova, Daria N Shalaeva, Daria V Dibrova, Armen Y Mulkidjanian
The P-loop fold nucleoside triphosphate (NTP) hydrolases (also known as Walker NTPases) function as ATPases, GTPases, and ATP synthases, are often of medical importance, and represent one of the largest and evolutionarily oldest families of enzymes. There is still no consensus on their catalytic mechanism. To clarify this, we performed the first comparative structural analysis of more than 3100 structures of P-loop NTPases that contain bound substrate Mg-NTPs or their analogues. We proceeded on the assumption that structural features common to these P-loop NTPases may be essential for catalysis...
September 22, 2022: Biomolecules
https://read.qxmd.com/read/35643887/ostub1-confers-salt-insensitivity-by-interacting-with-kinesin13a-to-stabilize-microtubules-and-ion-transporters-in-rice
#10
JOURNAL ARTICLE
Gaoming Chen, Wei Xuan, Pingzhi Zhao, Xiangmei Yao, Chao Peng, Yunlu Tian, Jian Ye, Baoxiang Wang, Jun He, Wenchao Chi, Jun Yu, Yuwei Ge, Jin Li, Zhaoyang Dai, Dayong Xu, Chunming Wang, Jianmin Wan
Salt stress is one of the major environmental factors limiting plant growth and development. Although microtubule (MT) organization is known to be involved in response to salt stress, few tubulin genes have been identified that confer salt insensitivity in plants. In this study, we identified a MT encoding gene, OsTUB1, that increased the survival rate of rice plants under salt stress by stabilizing MT organization and ion transporters. We found that OsTUB1 interacted with Kinesin13A protein, which was essential for OsTUB1-regulated MT organization under salt stress...
September 2022: New Phytologist
https://read.qxmd.com/read/35412274/studying-axonal-transport-in-the-brain-by-manganese-enhanced-magnetic-resonance-imaging-memri
#11
JOURNAL ARTICLE
Elaine L Bearer, Xiaowei Zhang, Russell E Jacobs
From the earliest notions of dynamic movements within the cell by Leeuwenhoek, intracellular transport in eukaryotes has been primarily explored by optical imaging. The giant axon of the squid became a prime experimental model for imaging transport due to its size, optical transparency, and physiological robustness. Even the biochemical basis of transport was identified using optical assays based on video microscopy of fractionated squid axoplasm. Discoveries about the dynamics and molecular components of the intracellular transport system continued in many model organisms that afforded experimental systems for optical imaging...
2022: Methods in Molecular Biology
https://read.qxmd.com/read/33598096/hybrid-method-for-representing-ions-in-implicit-solvation-calculations
#12
JOURNAL ARTICLE
Shengjie Sun, Chitra Karki, Yixin Xie, Yuejiao Xian, Wenhan Guo, Bruce Z Gao, Lin Li
Fast and accurate calculations of the electrostatic features of highly charged biomolecules such as DNA, RNA, and highly charged proteins are crucial and challenging tasks. Traditional implicit solvent methods calculate the electrostatic features quickly, but these methods are not able to balance the high net biomolecular charges effectively. Explicit solvent methods add unbalanced ions to neutralize the highly charged biomolecules in molecular dynamic simulations, which require more expensive computing resources...
2021: Computational and Structural Biotechnology Journal
https://read.qxmd.com/read/33481593/step-wise-hydration-of-magnesium-by-four-water-molecules-precedes-phosphate-release-in-a-myosin-motor
#13
JOURNAL ARTICLE
Mauro Lorenzo Mugnai, D Thirumalai
Molecular motors, such as myosin, kinesin, and dynein, convert the energy released by the hydrolysis of ATP into mechanical work, thus allowing them to undergo directional motion on cytoskeletal tracks. A pivotal step in the chemomechanical transduction in myosin motors occurs after they bind to the actin filament, which triggers the release of phosphate (Pi , product of ATP hydrolysis) and the rotation of the lever arm. Here, we investigate the mechanism of phosphate release in myosin VI using extensive molecular dynamics simulations involving multiple trajectories of several μs...
January 22, 2021: Journal of Physical Chemistry. B
https://read.qxmd.com/read/33472074/transcriptome-alterations-in-myotonic-dystrophy-frontal-cortex
#14
JOURNAL ARTICLE
Brittney A Otero, Kiril Poukalov, Ryan P Hildebrandt, Charles A Thornton, Kenji Jinnai, Harutoshi Fujimura, Takashi Kimura, Katharine A Hagerman, Jacinda B Sampson, John W Day, Eric T Wang
Myotonic dystrophy (DM) is caused by expanded CTG/CCTG repeats, causing symptoms in skeletal muscle, heart, and central nervous system (CNS). CNS issues are debilitating and include hypersomnolence, executive dysfunction, white matter atrophy, and neurofibrillary tangles. Here, we generate RNA-seq transcriptomes from DM and unaffected frontal cortex and identify 130 high-confidence splicing changes, most occurring only in cortex, not skeletal muscle or heart. Mis-spliced exons occur in neurotransmitter receptors, ion channels, and synaptic scaffolds, and GRIP1 mis-splicing modulates kinesin association...
January 19, 2021: Cell Reports
https://read.qxmd.com/read/32456460/architecture-of-the-ift-ciliary-trafficking-machinery-and-interplay-between-its-components
#15
JOURNAL ARTICLE
Kazuhisa Nakayama, Yohei Katoh
Cilia and flagella serve as cellular antennae and propellers in various eukaryotic cells, and contain specific receptors and ion channels as well as components of axonemal microtubules and molecular motors to achieve their sensory and motile functions. Not only the bidirectional trafficking of specific proteins within cilia but also their selective entry and exit across the ciliary gate is mediated by the intraflagellar transport (IFT) machinery with the aid of motor proteins. The IFT-B complex, which is powered by the kinesin-2 motor, mediates anterograde protein trafficking from the base to the tip of cilia, whereas the IFT-A complex together with the dynein-2 complex mediates retrograde protein trafficking...
May 26, 2020: Critical Reviews in Biochemistry and Molecular Biology
https://read.qxmd.com/read/32437854/coordinating-synaptic-signaling-with-crmp2
#16
REVIEW
Harrison Stratton, Lisa Boinon, Aubin Moutal, Rajesh Khanna
Synaptic transmission is a complex process, dysregulation of which underlies several neurological conditions. Collapsin response mediator protein 2 (CRMP2) is a microtubule associated protein expressed ubiquitously in the central nervous system. Identified initially in the context of Semaphorin 3A (Collapsin) induced growth cone collapse, more recent findings revealed the involvement of CRMP2 in ion channel trafficking, kinesin-dependent axonal transport and maintenance of intracellular calcium homeostasis...
July 2020: International Journal of Biochemistry & Cell Biology
https://read.qxmd.com/read/31968570/myosin-and-other-energy-transducing-atpases-structural-dynamics-studied-by-electron-paramagnetic-resonance
#17
REVIEW
Toshiaki Arata
The objective of this article was to document the energy-transducing and regulatory interactions in supramolecular complexes such as motor, pump, and clock ATPases. The dynamics and structural features were characterized by motion and distance measurements using spin-labeling electron paramagnetic resonance (EPR) spectroscopy. In particular, we focused on myosin ATPase with actin-troponin-tropomyosin, neural kinesin ATPase with microtubule, P-type ion-motive ATPase, and cyanobacterial clock ATPase. Finally, we have described the relationships or common principles among the molecular mechanisms of various energy-transducing systems and how the large-scale thermal structural transition of flexible elements from one state to the other precedes the subsequent irreversible chemical reactions...
January 20, 2020: International Journal of Molecular Sciences
https://read.qxmd.com/read/31597897/-architectures-and-functions-of-motor-proteins-underlying-the-intraflagellar-transport-machinery
#18
JOURNAL ARTICLE
Yohei Katoh
Primary cilia, which protrude from the surfaces of most human cells, function as cellular antennae that receive extracellular signals. To serve as antennae, primary cilia contain unique proteins, such as G-protein-coupled receptors and ion channels. Defects in the assembly and functions of primary cilia cause hereditary disorders with a wide range of symptoms, including cystic kidney and retinal degeneration. The assembly and maintenance of cilia depend on protein trafficking mediated by the intraflagellar transport (IFT) machinery, which contains three protein complexes (IFT-A, IFT-B, and BBSome) and two motor proteins (kinesin-2 and dynein-2 complex) and is composed of more than 40 subunits in total...
2019: Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
https://read.qxmd.com/read/31186137/cilia-function-is-associated-with-axon-initial-segment-morphology
#19
JOURNAL ARTICLE
Bingjie Wang, Lili Hu, Zehui Sun, Yan Zhang
Cilia, as key integrators of extracellular ligand-based signaling, play a critical role in signaling in the neuronal system. However, the function of cilia in neurons is largely unknown. In this study, we discovered that cilia morphology and ciliary protein localization were associated with axon initial segment (AIS) morphology, including length and location. Cilia morphological changes induced by the serotonin (5-HT) receptor 5-HT6R, intraflagellar transport 88 (Ift88) and kinesin family member 3A (KIF3A) altered AIS length and location...
June 8, 2019: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/31055762/single-molecule-in-vitro-reconstitution-assay-for-kinesin-1-driven-membrane-dynamics
#20
REVIEW
Wanqing Du, Qian Peter Su
Intracellular membrane dynamics, especially the nano-tube formation, plays important roles in vesicle transportation and organelle biogenesis. Regarding the regulation mechanisms, it is well known that during the nano-tube formation, motor proteins act as the driven force moving along the cytoskeleton, lipid composition and its associated proteins serve as the linkers and key mediators, and the vesicle sizes play as one of the important regulators. In this review, we summarized the in vitro reconstitution assay method, which has been applied to reconstitute the nano-tube dynamics during autophagic lysosomal regeneration (ALR) and the morphology dynamics during mitochondria network formation (MNF) in a mimic and pure in vitro system...
May 4, 2019: Biophysical Reviews
keyword
keyword
51368
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.