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https://www.readbyqxmd.com/read/28109187/antimicrobial-and-antioxidant-activities-of-saccharomyces-cerevisiae-ifst062013-a-potential-probiotic
#1
Md Fakruddin, Md Nur Hossain, Monzur Morshed Ahmed
BACKGROUND: Probiotic yeast has become a field of interest to scientists in recent years. METHODS: Conventional cultural method was employed to isolate and identify yeast and standard methods were used to determine different probiotic attributes, antimicrobial and antioxidant properties. RESULTS: This study reports potential probiotic properties of a strain of S. cerevisiae IFST 062013 isolated from fruit. The isolate is tolerant to a wide range of temperature and pH, high concentration of bile salt and NaCl, gastric juice, intestinal environment, α-amylase, trypsin and lysozyme...
January 21, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28109183/chloride-ion-transport-and-overexpression-of-tmem16a-in-a-guinea-pig-asthma-model
#2
Mitsuko Kondo, Mayoko Tsuji, Kaori Hara, Ken Arimura, Osamitsu Yagi, Etsuko Tagaya, Kiyoshi Takeyama, Jun Tamaoki
BACKGROUND: TMEM16A, a Ca-activated Cl channel, regulates various physiological functions such as mucin secretion. However, the role of TMEM16A in hypersecretion in asthma is not fully understood. OBJECTIVE: The aim of this study is to evaluate Cl ion transport via TMEM16A and determine the localization of TMEM16A in a guinea pig asthma model. METHODS: Guinea pigs were sensitized with OVA ip. on Day 1 and Day 8. On Day 22, we assessed OVA challenge-induced Cl ion transport in the sensitized tracheas ex vivo in an Ussing chamber, compared with the non-sensitized tracheas...
January 21, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28109159/activity-dependent-regulation-of-t-type-calcium-channels-by-submembrane-calcium-ions
#3
Magali Cazade, Isabelle Bidaud, Philippe Lory, Jean Chemin
Voltage-gated Ca(2+) channels are involved in numerous physiological functions and various mechanisms finely tune their activity, including the Ca(2+) ion itself. This is well exemplified by the Ca(2+)-dependent inactivation of L-type Ca(2+) channels, whose alteration contributes to the dramatic disease Timothy Syndrome. For T-type Ca(2+) channels, a long-held view is that they are not regulated by intracellular Ca(2+). Here we challenge this notion by using dedicated electrophysiological protocols on both native and expressed T-type Ca(2+) channels...
January 21, 2017: ELife
https://www.readbyqxmd.com/read/28108957/effect-of-temperature-on-surface-characteristics-of-nitrogen-ion-implanted-biocompatible-titanium
#4
Hossein Aghajani, Mohsen Sadeghpour Motlagh
It has been established that nitrogen implantation can modify metals surface properties such as corrosion, wear, friction, etc. Recent studies have shown that nitrogen implantation into titanium and its alloys leads to the formation of titanium nitride, which has interesting biological, chemical, and mechanical properties. In the present investigation, commercially pure biocompatible titanium implanted by nitrogen. Implantation of ions performed at 80 keV and a dose of 2.1 × 10(18) ions/cm(2) at four different temperatures of 300, 473, 673 and 873 k...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28108923/adsorption-of-nickel-ions-by-oleate-modified-magnetic-iron-oxide-nanoparticles
#5
Cécilia Magnet, Claire Lomenech, Charlotte Hurel, Pierre Reilhac, Françoise Giulieri, Anne-Marie Chaze, Jacques Persello, Pavel Kuzhir
In this work, magnetic nanoparticles of iron oxide (MNPs) were synthesized, and then the surface was recovered with an oleate double layer in order to investigate the ability of this material to adsorb nickel ions. First, the solution chemistry of oleate ions was investigated in order to determine the critical micellar concentration (CMC) value and the arrangements of ions above the CMC. Then, the synthesized oleate-modified MNP was characterized (TEM, DLS, XRD, FTIR, zeta potential, magnetometry). Finally, adsorption experiments were carried out as a function of pH and as a function of nickel concentration in 0...
January 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28108753/release-of-free-amino-acids-upon-oxidation-of-peptides-and-proteins-by-hydroxyl-radicals
#6
Fobang Liu, Senchao Lai, Haijie Tong, Pascale S J Lakey, Manabu Shiraiwa, Michael G Weller, Ulrich Pöschl, Christopher J Kampf
Hydroxyl radical-induced oxidation of proteins and peptides can lead to the cleavage of the peptide, leading to a release of fragments. Here, we used high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) and pre-column online ortho-phthalaldehyde (OPA) derivatization-based amino acid analysis by HPLC with diode array detection and fluorescence detection to identify and quantify free amino acids released upon oxidation of proteins and peptides by hydroxyl radicals. Bovine serum albumin (BSA), ovalbumin (OVA) as model proteins, and synthetic tripeptides (comprised of varying compositions of the amino acids Gly, Ala, Ser, and Met) were used for reactions with hydroxyl radicals, which were generated by the Fenton reaction of iron ions and hydrogen peroxide...
January 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28108654/revealing-the-role-of-the-product-metal-in-dna-polymerase-%C3%AE-catalysis
#7
Lalith Perera, Bret D Freudenthal, William A Beard, Lee G Pedersen, Samuel H Wilson
DNA polymerases catalyze a metal-dependent nucleotidyl transferase reaction during extension of a DNA strand using the complementary strand as a template. The reaction has long been considered to require two magnesium ions. Recently, a third active site magnesium ion was identified in some DNA polymerase product crystallographic structures, but its role is not known. Using quantum mechanical/ molecular mechanical calculations of polymerase β, we find that a third magnesium ion positioned near the newly identified product metal site does not alter the activation barrier for the chemical reaction indicating that it does not have a role in the forward reaction...
January 20, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28108412/deciphering-the-mechanistic-insight-into-the-stoichiometric-ratio-dependent-behavior-of-cu-ii-on-bsa-fibrillation
#8
Ajeet Singh, Poulami Datta, Lalit M Pandey
Various metal ions are recently implicated in protein aggregates and are associated with numerous neurodegenerative diseases. In the present work, we have scrutinized the effect of stoichiometric variation of Cu(II) on BSA fibrillation at physiological pH 7.4 through Thioflavin-T dye binding study, residual protein concentration, Fourier Transform Infrared spectroscopy (FTIR), Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) and Isothermal Titration Calorimetry (ITC). Fibrillation kinetics was studied through ThT fluorescence, which illustrated dependency on stoichiometric ratio of Cu(II)...
January 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28108264/iron-accumulation-and-microglia-activation-contribute-to-substantia-nigra-hyperechogenicity-in-the-6-ohda-induced-rat-model-of-parkinson-s-disease
#9
Yaqin Zhu, Bao Wang, Kai Tao, Hengli Yang, Yixiao Wang, Tian Zhou, Yilin Yang, Lijun Yuan, Xi Liu, Yunyou Duan
INTRODUCTION: This study aims to explain the mechanisms for the formation of sonographic features of Parkinson's disease (PD) using a 6-hydroxydopamine (6-OHDA) rat model of PD. The iron chelator deferiprone (DFP) was used in the PD model rat to examine the relationship between iron and the echo signal. METHODS: Rat models were created using stereotactic injections of 6-OHDA. DFP was administered intragastrically. Transcranial sonography (TCS) was performed to observe the substantia nigra (SN) echo signal of the brain...
January 6, 2017: Parkinsonism & related Disorders
https://www.readbyqxmd.com/read/28108075/catalytic-potential-of-selected-metal-ions-for-bioleaching-and-potential-techno-economic-and-environmental-issues-a-critical-review
#10
REVIEW
Ashish Pathak, Liam Morrison, Mark Gerard Healy
Bioleaching is considered to be a low-cost, eco-friendly technique for leaching valuable metals from a variety of matrixes. However, the inherent slow dissolution kinetics and low metal leaching yields have restricted its wider commercial applicability. Recent advancements in bio-hydrometallurgy have suggested that these critical issues can be successfully alleviated through the addition of a catalyst. The catalyzing properties of a variety of metals ions (Ag(+), Hg(++), Bi(+++), Cu(++), Co(++) etc.) during bioleaching have been successfully demonstrated...
January 6, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28107990/combined-use-of-peptide-ion-and-normalized-delta-scores-to-evaluate-milk-authenticity-by-ion-trap-based-proteomics-coupled-with-error-tolerant-searching
#11
Donatella Nardiello, Anna Natale, Carmen Palermo, Maurizio Quinto, Diego Centonze
A fundamental issue in proteomics is the peptide identification by database searching and the assessment of the goodness of fit between experimental and theoretical data. Despite the different number of ways to measure the quality of search results, the definition of a scoring criterion is still highly desirable in ion-trap based proteomics. Indeed, in order to fully take advantage of a low resolution MS/MS dataset, it is essential to strike a balance between greater information capture and reduced number of incorrect peptide assignments...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107983/characterization-of-inclusion-complexes-of-organic-ions-with-hydrophilic-hosts-by-ion-transfer-voltammetry-with-solvent-polymeric-membranes
#12
José Manuel Olmos, Eduardo Laborda, Joaquín Ángel Ortuño, Ángela Molina
The quantitative characterization of inclusion complexes formed in aqueous phase between organic ions and hydrophilic hosts by ion-transfer voltammetry with solvent polymeric membrane ion sensors is studied, both in a theoretical and experimental way. Simple analytical solutions are presented for the determination of the binding constant of the complex from the variation with the host concentration of the electrochemical signal. These solutions are valid for any voltammetric technique and for solvent polymeric membrane ion sensors comprising one polarisable interface (1PI) and also, for the first time, two polarisable interfaces (2PIs)...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107975/comparison-of-sample-preparation-strategies-for-target-analysis-of-total-thyroid-hormones-levels-in-serum-by-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#13
E Álvarez, Y Madrid, M D Marazuela
This paper describes a novel method based on liquid chromatography quadrupole time-of-flight-mass spectrometry (LC-QTOF-MS) for target analysis of total THs in serum. Several sample preparation strategies have been evaluated to reduce matrix effect (namely, HybridSPE cartridges, supported liquid extraction, SLE and solid phase extraction, SPE). Deproteinization and further clean-up with mixed-mode SPE was selected as the best strategy for sample preparation, since achieved the cleanest extracts and reduced ionization suppression effects (between -11 and -24%)...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107961/new-approach-of-a-transient-icp-ms-measurement-method-for-samples-with-high-salinity
#14
Christina Hein, Jonas Michael Sander, Ralf Kautenburger
In the near future it is necessary to establish a disposal for high level nuclear waste (HLW) in deep and stable geological formations. In Germany typical host rocks are salt or claystone. Suitable clay formations exist in the south and in the north of Germany. The geochemical conditions of these clay formations show a strong difference. In the northern ionic strengths of the pore water up to 5M are observed. The determination of parameters like Kd values during sorption experiments of metal ions like uranium or europium as homologues for trivalent actinides onto clay stones are very important for long term safety analysis...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107958/one-step-synthesis-of-graphitic-carbon-nitride-nanosheets-with-the-help-of-melamine-and-its-application-for-fluorescence-detection-of-mercuric-ions
#15
Qianfen Zhuang, Liming Sun, Yongnian Ni
A facile, simple, and relatively environment-friendly hydrothermal approach was developed for one-step synthesis of graphitic carbon nitride nanosheets (GCNNs) using melamine and sodium citrate as the precursors. The prepared GCNNs emit strong fluorescence with a high quantum yield of 48.3%. The GCNNs were then characterized by various techniques including transmission electron microscopy, atomic force microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and UV-Vis absorption spectroscopy...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107955/matrix-isolation-with-an-ion-transfer-device-for-interference-free-simultaneous-spectrophotometric-determinations-of-hexavalent-and-trivalent-chromium-in-a-flow-based-system
#16
Shin-Ichi Ohira, Koretaka Nakamura, Mitsuki Chiba, Purnendu K Dasgupta, Kei Toda
Chromium speciation by spectrophotometric determination of hexavalent chromium (Cr(VI)) with diphenylcarbazide (DPC) has several problems. These include: (1) the inability to directly detect trivalent chromium (Cr(III)) with DPC, (2) positive interference in Cr(VI) determination by other metal cations and (3) negative interference by any reducing agent present in the sample. These are addressed with an ion transfer device (ITD) in a flow injection analysis system. We previously developed the ITD for electrodialytic separations...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107952/lanthanide-coordination-polymer-probe-for-time-gated-luminescence-sensing-of-ph-in-undiluted-human-serum
#17
Yankai Huang, Baoxia Liu, Qi Shen, Xu Zhu, Yuanqiang Hao, Zhu Chang, Fang Xu, Peng Qu, Maotian Xu
Lanthanide coordination polymers (LCPs) have emerged as fascinating materials because of their specific structure and properties. In this work, utilizing hydrosoluble biomolecule of guanosine-5'-monophosphate (GMP) as bridging linker, lanthanide terbium ions (Tb(3+)) as metal nodes, and silver ions (Ag(+)) as sensitizers, we synthesized a pH responsive luminescent lanthanide CP probe of Tb/GMP/Ag. The probe possesses high luminescence due to the sensitization of Ag(+); While in alkaline solutions, Ag(+) in Tb/GMP/Ag immediately binds to OH(-), forming Ag2O precipitation and resulting in a distinct fluorescence quenching of Tb/GMP/Ag...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107944/lanthanide-to-quantum-dot-f%C3%A3-rster-resonance-energy-transfer-fret-application-for-immunoassay
#18
REVIEW
O A Goryacheva, N V Beloglazova, A M Vostrikova, M V Pozharov, A M Sobolev, I Yu Goryacheva
Förster resonance energy transfer (FRET) between lanthanide ion complexes (L) acting as donors and luminescent semiconductor quantum dots (QD) acting as acceptors is discussed in the terms of advantages and disadvantages for its application in immunoassay. L-QD-FRET is potentially a powerful tool that can be used to detect and confirm formation of immunocomplexes, but until now it had very limited practical analytical application. Therefore, the main aim of this review is to analyze all possibilities, advantages, and disadvantages of L-QD-FRET in immunoassay applications...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107934/tuning-sensitivity-of-a-simple-hydrazone-for-selective-fluorescent-turn-on-chemo-sensing-of-al-3-and-its-application-in-living-cells-imaging
#19
Faiz-Ur Rahman, Amjad Ali, Saira Khan Khalil, Rong Guo, Peng Zhang, Hui Wang, Zhan-Ting Li, Dan-Wei Zhang
Salicylaldehyde based hydrazones 2-((2-methyl-2-(pyridin-2-yl)hydrazono)methyl)phenol (SMPH), 4-methyl-2-((2-methyl-2-(pyridin-2-yl)hydrazono)methyl)phenol (MSMPH) and 4-fluoro-2-((2-methyl-2-(pyridin-2-yl)hydrazono)methyl)phenol (FSMPH) were prepared and structurally characterized. These hydrazones exhibited fluorescence "turn on" response toward Al(3+) in dimethyl formamide/water (DMF/H2O) (1: 1) mixture. SMPH showed enhanced emission (70 folds) as compared to MSMPH (46 folds) and FSMPH (28 folds) upon the addition of 3 equivalents of Al(3+)...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107923/a-sensitive-and-rapid-uflc-apci-ms-ms-bioanalytical-method-for-quantification-of-endogenous-and-exogenous-vitamin-k1-isomers-in-human-plasma-development-validation-and-first-application-to-a-pharmacokinetic-study
#20
Nagaraj Kumar Nannapaneni, Sunil S Jalalpure, Rajendraprasad Muppavarapu, Sunil Kumar Sirigiri
Due to lack of suitable bioanalytical methods in previous literature, for simultaneous estimation of Vitamin K1 isomers, in compliance with the current regulatory expectation, we aimed to develop a sensitive and rapid method with UFLC-APCI-MS/MS (ultrafast liquid chromatography - tandem mass spectrometry) using human plasma. A simple and cost effective procedure was implemented with the combination of protein precipitation and liquid extraction, to isolate the targets from plasma sample, while achieving an insignificant matrix effects and high recovery (≥88...
March 1, 2017: Talanta
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