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https://www.readbyqxmd.com/read/29786849/isolation-and-characterization-of-wheat-derived-nonspecific-lipid-transfer-protein-2-nsltp2
#1
Sara Bosi, Jessica Fiori, Giovanni Dinelli, Neil Rigby, Emanuela Leoncini, Cecilia Prata, Valeria Bregola, Ilaria Marotti, Roberto Gotti, Marina Naldi, Luca Massaccesi, Marco Malaguti, Paul Kroon, Silvana Hrelia
Numerous studies support the protective role of bioactive peptides against cardiovascular diseases. Cereals represent the primary source of carbohydrates, but they also contain substantial amounts of proteins, therefore representing a potential dietary source of bioactive peptides with nutraceutical activities. The analysis of wheat extracts purified by chromatographic techniques by means of HPLC-UV/nanoLC-nanoESI-QTOF allowed the identification of a signal of about 7 kDa which, following data base searches, was ascribed to a nonspecific lipid-transfer protein (nsLTP) type 2 from Triticum aestivum (sequence coverage of 92%)...
May 22, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29786609/comparison-of-trapped-charges-and-hysteresis-behavior-in-hbn-encapsulated-single-mos-sub-2-sub-flake-based-field-effect-transistors-on-sio-sub-2-sub-and-hbn-substrates
#2
Changhee Lee, Servin Rathi, Muhammad Atif Khan, Dongsuk Lim, Yunseob Kim, Sun Jin Yun, Doo-Hyeb Youn, Kenji Watanabe, Takashi Taniguchi, Gil-Ho Kim
Molybdenum disulfide (MoS<sub>2</sub>) based field effect transistors (FETs) are of considerable interest in electronic and opto-electronic applications but often have large hysteresis and threshold voltage instabilities. In this study, by using advanced transfer techniques, hexagonal boron nitride (hBN) encapsulated FETs based on a single, homogeneous and atomic-thin MoS<sub>2</sub> flake are fabricated on hBN and SiO<sub>2</sub> substrates...
May 22, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29785406/fluid-extravasation-in-shoulder-arthroscopic-surgery-a-systematic-review
#3
REVIEW
Muzammil Memon, Jeffrey Kay, Arian Gholami, Nicole Simunovic, Olufemi R Ayeni
Background: Arthroscopic surgery of the shoulder joint has become increasingly more common given its advantages over open surgery; however, one rare but potentially life-threatening complication is fluid extravasation into the surrounding tissues, causing edema, respiratory compromise, abnormal results on laboratory blood tests, and possibly death. Currently, no systematic review exists that summarizes the existing clinical research on this topic. Purpose: To perform a systematic review on fluid extravasation as a complication of shoulder arthroscopic surgery, specifically assessing clinical presentation, risk factors, management, and outcomes...
May 2018: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29785186/platelets-activated-by-the-anti-%C3%AE-2gpi-%C3%AE-2gpi-complex-release-micrornas-to-inhibit-migration-and-tube-formation-of-human-umbilical-vein-endothelial-cells
#4
Yanfen Zhang, Wenjing Zhang, Caijun Zha, Yanhong Liu
Background: Patients with anti-β2GPI antibodies display significantly higher platelet activation/aggregation and vascular endothelial cell damage. The mechanism underlying the correlation between platelet activation, vascular endothelial cell dysfunctions and anti-β2GPI antibodies remains unknown. Methods: In this study, we derived miR-96 and -26a from platelets activated by the anti-β2GPI/β2GPI complex and explored their role in modulating human umbilical vein endothelial cell (HUVEC) migration and tube formation...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29784403/enzymatic-amplification-of-oligonucleotides-in-paper-substrates
#5
Abootaleb Sedighi, Ulrich J Krull
Several solution-based methods have recently been adapted for use in paper substrates for enzymatic amplification to increase the number of copies of DNA sequences. There is limited information available about the impact of a paper matrix on DNA amplification by enzymatic processes, and about how to optimize conditions to maximize yields. The work reported herein provides insights about the impact of physicochemical properties of a paper matrix, using nuclease-assisted amplification by exonuclease III and nicking endonuclease Nt...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29784400/in-situ-immobilization-of-layered-double-hydroxides-onto-cotton-fiber-for-solid-phase-extraction-of-fluoroquinolone-drugs
#6
Xuemei Wang, Wei Zhou, Chenlu Wang, Zilin Chen
Cotton fiber was proposed as a biodegradable support material. The properties like high specific area, hygroscopicity and adjustable shape make it be an idea substrate for solid phase extraction (SPE). In this work, inorganic layered double hydroxides (LDHs) were in situ immobilized onto cotton fiber through the linker of polydopamine as stationary phase. Uniform NiAl-LDHs crystal with nanostructure was observed on cotton fiber after one-step synthesis through urea hydrolytic method. The cotton fiber can not only increase the specific area and mass transfer rate, but also prevent aggregation and blocking of the freestanding LDHs crystal...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29784060/neonatal-mortality-in-the-case-of-felege-hiwot-referral-hospital-bahir-dar-amhara-regional-state-north-west-ethiopia-2016-a-one-year-retrospective-chart-review
#7
Tilahun Tewabe, Yenatfanta Mehariw, Eyerusalem Negatie, Bertukan Yibeltal
BACKGROUND: Ethiopia is among the countries with the highest neonatal mortality with the rate of 37 deaths per 1000 live births. In spite of many efforts by the government and other partners, non significant decline has been achieved over the last 15 years. Thus, identifying the prevalence and associated factors of neonatal mortality is very crucial for policy and program improvement. This study was designed to assess neonatal mortality rate in Felege Hiwot referral hospital, North West Ethiopia...
May 21, 2018: Italian Journal of Pediatrics
https://www.readbyqxmd.com/read/29783988/tolerogenic-bone-marrow-derived-dendritic-cells-induce-neuroprotective-regulatory-t-cells-in-a-model-of-parkinson-s-disease
#8
Charles R Schutt, Howard E Gendelman, R Lee Mosley
BACKGROUND: Administration of granulocyte-macrophage colony-stimulating factor (GM-CSF) increases regulatory T cell (Treg) number and function with control of neuroinflammation and neuronal protection in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of Parkinson's disease (PD). Recently, we demonstrated in an early phase 1 clinical trial that GM-CSF also improves motor skills in PD patients. However, the mechanisms of Treg induction and its effects on neuroprotective responses remain unknown...
May 21, 2018: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/29783764/intensity-of-multilingual-language-use-predicts-cognitive-performance-in-some-multilingual-older-adults
#9
Anna Pot, Merel Keijzer, Kees de Bot
Cognitive advantages for bilinguals have inconsistently been observed in different populations, with different operationalisations of bilingualism, cognitive performance, and the process by which language control transfers to cognitive control. This calls for studies investigating which aspects of multilingualism drive a cognitive advantage, in which populations and under which conditions. This study reports on two cognitive tasks coupled with an extensive background questionnaire on health, wellbeing, personality, language knowledge and language use, administered to 387 older adults in the northern Netherlands, a small but highly multilingual area...
May 19, 2018: Brain Sciences
https://www.readbyqxmd.com/read/29783743/cell-propagation-of-cholera-toxin-cta-adp-ribosylating-factor-by-exosome-mediated-transfer
#10
Cristiana Zanetti, Angelo Gallina, Alessia Fabbri, Sofia Parisi, Angela Palermo, Katia Fecchi, Zaira Boussadia, Maria Carollo, Mario Falchi, Luca Pasquini, Maria Luisa Fiani, Massimo Sargiacomo
In this study, we report how the cholera toxin (CT) A subunit (CTA), the enzyme moiety responsible for signaling alteration in host cells, enters the exosomal pathway, secretes extracellularly, transmits itself to a cell population. The first evidence for long-term transmission of CT's toxic effect via extracellular vesicles was obtained in Chinese hamster ovary (CHO) cells. To follow the CT intracellular route towards exosome secretion, we used a novel strategy for generating metabolically-labeled fluorescent exosomes that can be counted by flow cytometry assay (FACS) and characterized...
May 19, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29783037/premature-progesterone-rise-on-day-of-hcg-negatively-correlated-with-live-birth-rate-in-ivf-cycles-an-analysis-of-1-022-cycles
#11
Lepage Julien, Keromnès Gwenola, Sylvie Epelboin, Luton Dominique, Yazbeck Chadi
OBJECTIVE: To investigate the relationship between serum P levels on the day of hCG administration and pregnancy outcomes in patients undergoing IVF. DESIGN: Retrospective study SETTING: Teaching hospital PATIENTS: A total of 1022 IVF-ICSI cycles, frozen embryo transfer excluded INTERVENTION(S):: Patients- all types of responder - underwent IVF with agonist or antagonist protocols. Clinical outcomes of IVF were analyzed according to plasma P levels. MAIN OUTCOME MEASURE(S): Ongoing pregnancy rates...
May 18, 2018: Journal of Gynecology Obstetrics and Human Reproduction
https://www.readbyqxmd.com/read/29781945/appropriateness-of-pediatric-orthopaedic-transfers-to-a-level-1-hospital
#12
Jaclyn McKenna, David Novikov, Amanda Pawlak, Jihye Park, Jie Yang, Hariharan Iyer, James Barsi
OBJECTIVE: The purpose of this study was to compare a cohort of transferred pediatric orthopaedic patients with orthopaedic patients who primarily presented to a Level 1 pediatric emergency department to identify risk factors for transfer. DESIGN: Retrospective cohort study. SETTING: This study was performed at a level one trauma center in New York. PATIENTS: The cohort consisted of patients under age 18 who presented to one level 1 pediatric trauma center between January 1, 2013 - December 31, 2013 with an orthopaedic fracture diagnosis code (ICD-9 805...
May 16, 2018: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/29781209/oral-phase-dysphagia-in-facial-onset-sensory-and-motor-neuronopathy
#13
Mitsuru Watanabe, Wataru Shiraishi, Ryo Yamasaki, Noriko Isobe, Motohiro Sawatsubashi, Ryuji Yasumatsu, Takashi Nakagawa, Jun-Ichi Kira
INTRODUCTION: Facial onset motor and sensory neuronopathy (FOSMN) is a rare disease whose cardinal features are initial asymmetrical facial sensory deficits followed by bulbar symptoms and spreading of sensory and motor deficits from face to scalp, neck, upper trunk, and upper extremities in a rostral-caudal direction. Although bulbar involvement is frequently observed in FOSMN, dysphagia in these patients has not been fully described. In this study, we aimed to characterize dysphagia as a prognostic factor in FOSMN by investigating our institutional case series...
May 21, 2018: Brain and Behavior
https://www.readbyqxmd.com/read/29781115/c-h-borylation-cross-coupling-forms-twisted-donor-acceptor-compounds-exhibiting-donor-dependent-delayed-emission
#14
Michael Ingleson, Daniel Crossley, Pakapol Kulapichitr, Michael Turner, Joseph McDouall, James Radcliffe, Jay Dunsford, Inigo Vitorica-Yrezabal, Rachel Kahan, Adam Woodward
Benzothiadiazole (BT) directed C-H borylation using BCl3, followed by B-Cl hydrolysis and Suzuki-Miyaura cross coupling enables facile access to twisted donor acceptor compounds. A subsequent second C-H borylation step provides, on arylation of boron, access to borylated highly twisted D-A compounds with a reduced band gap, or on B-Cl hydrolysis/cross coupling to twisted D-A-D compounds. Photophysical studies revealed that in this series there is long lifetime emission only when the donor is triphenylamine...
May 21, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29780875/amyloid-%C3%AE-peptides-are-differentially-vulnerable-to-preanalytical-surface-exposure-an-effect-incompletely-mitigated-by-the-use-of-ratios
#15
Jamie Toombs, Martha S Foiani, Henrietta Wellington, Ross W Paterson, Charles Arber, Amanda Heslegrave, Michael P Lunn, Jonathan M Schott, Selina Wray, Henrik Zetterberg
Introduction: We tested the hypothesis that the amyloid β (Aβ) peptide ratios are more stable than Aβ42 alone when biofluids are exposed to two preanalytical conditions known to modify measurable Aβ concentration. Methods: Human cerebrospinal fluid (CSF) and culture media (CM) from human cortical neurons were exposed to a series of volumes and polypropylene surfaces. Aβ42 , Aβ40 , and Aβ38 peptide concentrations were measured using a multiplexed electrochemiluminescence immunoassay...
2018: Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring
https://www.readbyqxmd.com/read/29780807/a-critical-review-of-the-bacterial-baptism-hypothesis-and-the-impact-of-cesarean-delivery-on-the-infant-microbiome
#16
REVIEW
Lisa F Stinson, Matthew S Payne, Jeffrey A Keelan
Numerous studies suggest that infants delivered by cesarean section are at a greater risk of non-communicable diseases than their vaginal counterparts. In particular, epidemiological studies have linked Cesarean delivery with increased rates of asthma, allergies, autoimmune disorders, and obesity. Mode of delivery has also been associated with differences in the infant microbiome. It has been suggested that these differences are attributable to the "bacterial baptism" of vaginal birth, which is bypassed in cesarean deliveries, and that the abnormal establishment of the early-life microbiome is the mediator of later-life adverse outcomes observed in cesarean delivered infants...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29780778/impact-of-presence-of-antiphospholipid-antibodies-on-in-vitro-fertilization-outcome
#17
Yeon Hee Hong, Se Jeong Kim, Kyoung Yong Moon, Seul Ki Kim, Byung Chul Jee, Won Don Lee, Seok Hyun Kim
Objective: To investigate prevalence of antiphospholipid antibody (APA) in Korean infertile women undergoing the first in vitro fertilization (IVF) treatment and to evaluate the influence of APA on the subsequent IVF outcomes. Method: Two hundred nineteen infertile women who destined the first IVF were prospectively enrolled in 2 infertility centers. Male factor or uterine factor infertility and women with past or current endocrine or immunologic disorders were completely excluded...
May 2018: Obstetrics & Gynecology Science
https://www.readbyqxmd.com/read/29780473/understanding-the-charge-transport-properties-of-redox-active-metal-organic-conjugated-wires
#18
Donglei Bu, Yingqi Xiong, Ying Ning Tan, Miao Meng, Paul J Low, Dai-Bin Kuang, Chun Y Liu
Layer-by-layer assembly of the dirhodium complex [Rh2 (O2 CCH3 )4 ] (Rh2 ) with linear N , N '-bidentate ligands pyrazine (LS ) or 1,2-bis(4-pyridyl)ethene (LL ) on a gold substrate has developed two series of redox active molecular wires, (Rh2 LS ) n @Au and (Rh2 LL ) n @Au ( n = 1-6). By controlling the number of assembling cycles, the molecular wires in the two series vary systematically in length, as characterized by UV-vis spectroscopy, cyclic voltammetry and atomic force microscopy. The current-voltage characteristics recorded by conductive probe atomic force microscopy indicate a mechanistic transition for charge transport from voltage-driven to electrical field-driven in wires with n = 4, irrespective of the nature and length of the wires...
April 14, 2018: Chemical Science
https://www.readbyqxmd.com/read/29780396/effects-of-antimony-stress-on-photosynthesis-and-growth-of-acorus-calamus
#19
Xiujie Zhou, Chongyu Sun, Pengfei Zhu, Fei Liu
This study was aimed to explore that effects of Sb on physiological parameters of Acorus calamus and the possibility of using A. calamus as a remediation plant. A. calamus potted experiments were conducted using different concentrations (0, 250, 500, 1000, and 2000 mg/kg) of antimony potassium tartrate (Sb3+ ) (marked as CK, T1 , T2 , T3 , and T4 , respectively) and potassium pyroantimonate (Sb5+ ) (marked as CK, T'1 , T'2 , T'3 , and T'4 , respectively). The effects of Sb stress (Sb3+ and Sb5+ ) on leaf photosynthetic pigments, biomass, photosynthetic characteristics and chlorophyll fluorescence parameters of potted A...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29780273/ectopic-ring-zinc-finger-gene-from-hot-pepper-induces-totally-different-genes-in-lettuce-and-tobacco
#20
Mahipal Singh Kesawat, Dong Kyun Kim, Naheed Zeba, Mi Chung Suh, Xinli Xia, Choo Bong Hong
Advances in molecular biology have improved crops through transferring genes from one organism to new hosts, and these efforts have raised concerns about potential unexpected outcomes. Here, we provide evidence that a gene with a specific function in one organism can yield completely different effects in a new host. CaRZFP1 is a C3HC4-type RING zinc finger protein gene previously isolated from a cDNA library for heat-stressed hot pepper. In our previous work investigating in vivo CaRZFP1 function, we transferred CaRZFP1 into tobacco; transgenic tobacco exhibited enhanced growth and tolerance to abiotic stresses...
2018: Molecular Breeding: New Strategies in Plant Improvement
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