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https://www.readbyqxmd.com/read/27919704/global-transcriptome-analysis-of-halolamina-sp-to-decipher-the-salt-tolerance-in-extremely-halophilic-archaea
#1
Aslıhan Kurt-Kızıldoğan, Büşra Abanoz, Sezer Okay
Extremely halophilic archaea survive in the hypersaline environments such as salt lakes or salt mines. Therefore, these microorganisms are good sources to investigate the molecular mechanisms underlying the tolerance to high salt concentrations. In this study, a global transcriptome analysis was conducted in an extremely halophilic archaeon, Halolamina sp. YKT1, isolated from a salt mine in Turkey. A comparative RNA-seq analysis was performed using YKT1 isolate grown either at 2.7M NaCl or 5.5M NaCl concentrations...
December 2, 2016: Gene
https://www.readbyqxmd.com/read/27917719/prevention-and-treatment-of-postoperative-endophthalmitis-using-povidone-iodine
#2
Hiroyuki Shimada, Hiroyuki Nakashizuka, Andrzej Grzybowski
Postoperative endophthalmitis is typically caused by patient's conjunctival bacterial flora or contaminated solutions and instruments. Visual outcome is often poor in severe cases, and treatment is especially difficult when endophthalmitis is caused by multidrug resistant bacteria. Povidone-iodine is used worldwide due to its wide-spectrum antimicrobial activity, absence of resistant bacteria to povidone-iodine, and low cost. Furthermore, the effective concentration against microorganisms and safe concentration for ocular tissues have been well established...
December 4, 2016: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/27917249/perspectives-in-flow-based-microfluidic-gradient-generators-for-characterizing-bacterial-chemotaxis
#3
Christopher J Wolfram, Gary W Rubloff, Xiaolong Luo
Chemotaxis is a phenomenon which enables cells to sense concentrations of certain chemical species in their microenvironment and move towards chemically favorable regions. Recent advances in microbiology have engineered the chemotactic properties of bacteria to perform novel functions, but traditional methods of characterizing chemotaxis do not fully capture the associated cell motion, making it difficult to infer mechanisms that link the motion to the microbiology which induces it. Microfluidics offers a potential solution in the form of gradient generators...
November 2016: Biomicrofluidics
https://www.readbyqxmd.com/read/27916134/underway-analysis-of-nanomolar-dissolved-reactive-phosphorus-in-oligotrophic-seawater-with-automated-on-line-solid-phase-extraction-and-spectrophotometric-system
#4
Jian Ma, Yuan Yuan, Dongxing Yuan
The automated in-field determination of trace phosphate (and other nutrients) is highly valuable for studying nutrient dynamics and cycling in oligotrophic oceans. Here, we report an automated portable analyzer for week-long underway analysis of nanomolar dissolved reactive phosphorus (DRP) in seawater. The method is based on classic phosphomolybdenum blue (PMB) chemistry combined with on-line solid phase extraction (SPE) and flow analysis. Under optimized conditions, the formed PMB from sample is automatically concentrated on a hydrophilic lipophilic balanced (HLB) copolymer SPE...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27913364/an-intelligible-risk-stratification-model-based-on-pairwise-and-size-constrained-kmeans
#5
Longfei Han, Senlin Luo, Huaiqing Wang, Limin Pan, Xincheng Ma, Tiemei Zhang
Having a system to stratify individuals according to risk is key to clinical disease prevention, this allows individuals identified at different risk tiers benefit from further investigation and intervention. But the same risk score estimated for two different persons doesn't mean they need the same further investigation or represent the similarity health condition between two persons. Meanwhile, users still does not know a prior what most of the risk tiers are, and how many tiers should be found in risk stratification...
November 29, 2016: IEEE Journal of Biomedical and Health Informatics
https://www.readbyqxmd.com/read/27913346/automatic-design-of-high-sensitivity-color-filter-arrays-with-panchromatic-pixels
#6
Jia Li, Chenyan Bai, Zhouchen Lin, Jian Yu
In most of existing digital cameras, color images have to be reconstructed from raw images which only have one color sensed at each pixel, as their imaging sensors are covered by color filter arrays (CFAs). At each pixel a CFA usually allows only a portion of the light spectrum to pass through and thereby reduces the light sensitivity of pixels. To address this issue, previous works have explored adding panchromatic pixels into CFAs. However, almost all existing methods assign panchromatic pixels empirically, making the designed CFAs prone to aliasing artifacts...
December 1, 2016: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/27912126/evaluation-of-the-inactivation-effect-of-triton-x-100-on-ebola-virus-infectivity
#7
Francesca Colavita, Serena Quartu, Eleonora Lalle, Licia Bordi, Daniele Lapa, Silvia Meschi, Antonella Vulcano, Antonietta Toffoletti, Eugenio Bordi, Maria Grazia Paglia, Antonino Di Caro, Giuseppe Ippolito, Maria Rosaria Capobianchi, Concetta Castilletti
BACKGROUND: The recent Ebola virus disease outbreak occurred in West Africa since December 2013 highlighted the need of appropriate virus inactivation procedures to be set up to allow the necessary processing of specimens outside BSL-4 facilities and to perform laboratory tests without affecting clinical decisions. For this purpose, international guidelines suggest the pre-treatment of the samples with Triton X-100. OBJECTIVES: Due to the limited scientific evidence about the efficacy of Triton X-100 on enveloped-viruses, the aim of this work was to evaluate the effect of Triton X-100 on the virus infectivity and to establish the optimal conditions for its use...
November 22, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27910397/enhanced-magnetic-field-probe-array-for-improved-excluded-flux-calculations-on-the-c-2u-advanced-beam-driven-field-reversed-configuration-plasma-experiment
#8
T Roche, M C Thompson, R Mendoza, I Allfrey, E Garate, J Romero, J Douglass
External flux conserving coils were installed onto the exterior of the C-2U [M. W. Binderbauer et al., Phys. Plasmas 22, 056110 (2015)] confinement vessel to increase the flux confinement time of the system. The 0.5 in. stainless steel vessel wall has a skin time of ∼5 ms. The addition of the external copper coils effectively increases this time to ∼7 ms. This led to better-confined/longer-lived field-reversed configuration (FRC) plasmas. The fringing fields generated by the external coils have the side effect of rendering external field measurements invalid...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27909643/source-control-and-graft-preservation-using-negative-pressure-wound-therapy-with-antibiotic-instillation-a-case-report
#9
Victoria Gilbert, Timothy Kelly, Robert Grossi
Negative pressure wound therapy (NPWT) is widely used to treat many types of complex wounds, and the advent of the instillation and dwell time (NPWTi-d) technique has enhanced this system with the addition of automated treatment with topical solutions. In the field of vascular surgery, NPWT is utilized to help close wounds over underlying grafts; however keeping these wounds free of infection and avoiding large reoperation when infection occurs remains a challenge. In this case report we present a patient who required acute intervention for limb ischemia, with a large wound created in the groin for anastomosis of a prosthetic graft bypass...
October 31, 2016: Curēus
https://www.readbyqxmd.com/read/27908012/pt-symmetric-couplers-with-competing-cubic-quintic-nonlinearities
#10
Gennadiy Burlak, Salomon Garcia-Paredes, Boris A Malomed
We introduce a one-dimensional model of the parity-time ( PT)-symmetric coupler, with mutually balanced linear gain and loss acting in the two cores, and nonlinearity represented by the combination of self-focusing cubic and defocusing quintic terms in each core. The system may be realized in optical waveguides, in the spatial and temporal domains alike. Stationary solutions for PT-symmetric solitons in the systems are tantamount to their counterparts in the ordinary coupler with the cubic-quintic nonlinearity, where the spontaneous symmetry breaking of solitons is accounted for by bifurcation loops...
November 2016: Chaos
https://www.readbyqxmd.com/read/27907030/sensitive-and-simplified-detection-of-antibiotic-influence-on-the-dynamic-and-versatile-changes-of-fecal-short-chain-fatty-acids
#11
Xiaoya Zhao, Zhenzuo Jiang, Fan Yang, Yan Wang, Xiumei Gao, Yuefei Wang, Xin Chai, Guixiang Pan, Yan Zhu
Short-chain fatty acids (SCFAs), produced by anaerobic fermentation of mainly indigestible dietary carbohydrates by gut microbiota, have a profound influence on intestinal function and host energy metabolism. Antibiotics may seriously disturb the balance of fecal SCFAs. To evaluate the impacts of antibiotics on fecal SCFAs produced by gut microbiota, a simple, reproducible and accurate gas chromatography (GC) method, which can simultaneously analyze seven SCFAs in fecal samples, was developed and validated...
2016: PloS One
https://www.readbyqxmd.com/read/27906775/improving-liver-allocation-using-optimized-neighborhoods
#12
Vikram Kilambi, Sanjay Mehrotra
BACKGROUND: Geographic disparities persist in access to liver transplantation. Candidates with similar urgency experience varying opportunities for transplants across the US. Policymakers are poised to act and 1 proposal entails reorganizing the current OPTN of 11 regions into 8 districts. However, redistricting has the shortcomings that OPOs are disconnected from their immediate neighbors by district borders and that it is not easily responsive to uncertainty resulting from variability in donor and listing rates...
October 7, 2016: Transplantation
https://www.readbyqxmd.com/read/27905481/investigating-the-highest-melting-temperature-materials-a-laser-melting-study-of-the-tac-hfc-system
#13
Omar Cedillos-Barraza, Dario Manara, K Boboridis, Tyson Watkins, Salvatore Grasso, Daniel D Jayaseelan, Rudy J M Konings, Michael J Reece, William E Lee
TaC, HfC and their solid solutions are promising candidate materials for thermal protection structures in hypersonic vehicles because of their very high melting temperatures (>4000 K) among other properties. The melting temperatures of slightly hypostoichiometric TaC, HfC and three solid solution compositions (Ta1-xHfxC, with x = 0.8, 0.5 and 0.2) have long been identified as the highest known. In the current research, they were reassessed, for the first time in the last fifty years, using a laser heating technique...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27905194/constructing-a-novel-dendron-for-the-self-host-blue-emitter-with-tadf-feature-solution-processed-nondoped-oleds-with-bipolar-charge-transfer-and-stable-color-purity
#14
Xinxin Ban, Baoping Lin, Wei Jiang, Yueming Sun
The self-host thermally activated delayed fluorescence materials have recently been identified as the effective emitters for solution-processed nondoped OLEDs. However, except for carbazole unit, few novel dendrons were developed to build the self-host TADF emitters. This study reports two self-host blue materials, tbCz-SO and poCz-SO, with the same TADF emissive core and different dendrons. The influence of the peripheral dendrons on the photophysical properties and electroluminescent performances of the self-host materials were systematically investigated...
November 30, 2016: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/27901529/a-cationic-iridium-complex-meets-an-electron-transporting-counter-anion-enhanced-performances-of-solution-processed-phosphorescent-light-emitting-diodes
#15
Ningzi Luo, Ying Lan, Ruiren Tang, Lei He, Lian Duan
An electron-transporting counter-anion, OXD-7-SO3(-), was developed for a blue-green-emitting cationic iridium complex, which showed superior device performance in solution-processed phosphorescent light-emitting diodes over its counterpart complex with a PF6(-) counter-anion, due to the facilitated electron-injection/transport and more balanced carrier recombination imparted by OXD-7-SO3(-).
November 30, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27901092/a-microfluidic-platform-to-design-crosslinked-hyaluronic-acid-nanoparticles-chanps-for-enhanced-mri
#16
Maria Russo, Paolo Bevilacqua, Paolo Antonio Netti, Enza Torino
Recent advancements in imaging diagnostics have focused on the use of nanostructures that entrap Magnetic Resonance Imaging (MRI) Contrast Agents (CAs), without the need to chemically modify the clinically approved compounds. Nevertheless, the exploitation of microfluidic platforms for their controlled and continuous production is still missing. Here, a microfluidic platform is used to synthesize crosslinked Hyaluronic Acid NanoParticles (cHANPs) in which a clinically relevant MRI-CAs, gadolinium diethylenetriamine penta-acetic acid (Gd-DTPA), is entrapped...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27900717/intravenous-fluids-balancing-solutions
#17
REVIEW
Ewout J Hoorn
The topic of intravenous (IV) fluids may be regarded as "reverse nephrology", because nephrologists usually treat to remove fluids rather than to infuse them. However, because nephrology is deeply rooted in fluid, electrolyte, and acid-base balance, IV fluids belong in the realm of our specialty. The field of IV fluid therapy is in motion due to the increasing use of balanced crystalloids, partly fueled by the advent of new solutions. This review aims to capture these recent developments by critically evaluating the current evidence base...
November 29, 2016: Journal of Nephrology
https://www.readbyqxmd.com/read/27896352/high-throughput-metabolomics-approach-reveals-new-mechanistic-insights-for-drug-response-of-phenotypes-of-geniposide-towards-alcohol-induced-liver-injury-by-using-liquid-chromatography-coupled-to-high-resolution-mass-spectrometry
#18
Tianlei Zhang, Aihua Zhang, Shi Qiu, Hui Sun, Ying Han, Yu Guan, Xijun Wang
Alcohol-induced liver injury (ALD) shows obvious metabolic disorders, categorized by a wide range of metabolite abnormalities. High-throughput metabolomics technology appears to be an appropriate solution. In this study, a urine metabolic profile was assessed using a UPLC-Q-TOF/HDMS (liquid chromatography coupled to high resolution mass spectrometry) approach to investigate the underlying molecular mechanisms of ALD and the therapeutic effect of geniposide. The endogenous low-molecular-weight metabolites in the mouse model of ALD were observed and 48 specific biomarkers were identified...
November 29, 2016: Molecular BioSystems
https://www.readbyqxmd.com/read/27896264/improving-fidelity-of-translation-of-the-stepping-on-falls-prevention-program-through-root-cause-analysis
#19
Jane E Mahoney, Vicki L Gobel, Terry Shea, Jodi Janczewski, Sandy Cech, Lindy Clemson
BACKGROUND: Fidelity monitoring is essential with implementation of complex health interventions, but there is little description of how to use results of fidelity monitoring to improve the draft program package prior to widespread dissemination. Root cause analysis (RCA) provides a systematic approach to identifying underlying causes and devising solutions to prevent errors in complex processes. Its use has not been described in implementation science. METHODS: Stepping On (SO) is a small group, community-based intervention that has been shown to reduce falls by 31%...
2016: Frontiers in Public Health
https://www.readbyqxmd.com/read/27893932/density-driven-free-convection-model-for-isotopically-fractionated-geogenic-nitrate-in-sabkha-brine
#20
Warren W Wood, J K Böhlke
Subsurface brines with high nitrate (NO3(-) ) concentration are common in desert environments as atmospheric nitrogen is concentrated by the evaporation of precipitation and little nitrogen uptake. However, in addition to having an elevated mean concentration of ∼525 mg/L (as N), NO3- in the coastal sabkhas of Abu Dhabi is enriched in (15) N (mean δ(15) N ∼17‰), which is an enigma. A NO3(-) solute mass balance analysis of the sabkha aquifer system suggests that more than 90% of the nitrogen is from local atmospheric deposition and the remainder from ascending brine...
November 28, 2016: Ground Water
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