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https://www.readbyqxmd.com/read/29156293/fe-iii-sulfide-interaction-in-globins-characterization-and-quest-for-a-putative-fe-iv-sulfide-species
#1
Augustin C Mot, Cristina Bischin, Grigore Damian, Amr A A Attia, Emese Gal, Nicoleta Dina, Nicolae Leopold, Radu Silaghi-Dumitrescu
The present study reports findings regarding the contrast between H2S interaction with bovine hemoglobin (Hb) and horse heart myoglobin (Mb), in terms of binding and dissociation kinetics, affinities, and mechanism. At pH9.5, oxidation of ferric-sulfide adducts in presence of no free sulfide, using hexachloroiridate as oxidant is examined using stopped-flow UV-vis, EPR, vibrational spectroscopy and mass spectrometry. Oxidation of the ferric-sulfide adduct in such conditions occurs with a putative unstable Fe(IV)-sulfide adduct as intermediate that finally leads to a paramagnetic ferric species with distinct EPR features...
November 10, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29154772/endogenous-peroxidases-in-sputum-interfere-with-horse-radish-peroxidase-based-elisas
#2
Lisa Ha-Yeon Kim, Karin Plaza, Sruthi R Thomas, Christina Draijer, Katherine Radford, Marc Peters-Golden, Manali Mukherjee, Parameswaran Nair
Peroxidase-based immunoassays are commonly used for detecting inflammatory mediators in biological samples. We suggest caution while interpreting assays particularly in sputum samples that have endogenous peroxidases like eosinophil peroxidase (EPX), which may interact with a horseradish peroxidase (HRP)-based ELISA. Using IL-8 as an example, we demonstrate that values generated with an HRP-ELISA (n=47) show significant positive correlation with the sputum EPX content (r=0.6, P=0.0004), which can be misconstrued to be affiliated with an eosinophilic event...
November 14, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29154707/objective-evaluation-of-the-systemic-effects-of-topical-application-of-1-atropine-sulfate-ophthalmic-solution-in-healthy-horses
#3
Rita F Wehrman, Anne J Gemensky-Metzler, Ashley E Zibura, Amelia B Nyhart, Heather L Chandler
OBJECTIVE To determine the safety of topical administration of 1% atropine ophthalmic solution in healthy horses by objectively measuring gastrointestinal transit time. DESIGN Randomized, masked, controlled crossover study. ANIMALS 6 adult geldings. PROCEDURES Horses were randomly assigned (3/group) to first receive topical treatment of the left eye with 1% atropine or artificial tears solution; the right eye was left untreated. After 24 hours of treatment every 6 hours, 200 nontoxic beads were administered to each horse via nasogastric intubation and treatment frequency was decreased to every 12 hours for 4 more days...
December 1, 2017: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/29154693/epidemiology-of-injury-due-to-race-day-jockey-falls-in-professional-flat-and-jump-horse-racing-in-ireland-2011-2015
#4
Siobhan O'Connor, Giles Warrington, Adrian McGoldrick, SarahJane Cullen
CONTEXT:   Professional horse racing is considered a high-risk sport, yet the last analysis of fall and injury incidence in this sport in Ireland was completed between 1999 and 2006. OBJECTIVE:   To provide an updated analysis of the fall and injury incidence in professional flat and jump horse racing in Ireland from 2011 through 2015, compare it with the previous analysis, and detail the specific types and locations of injuries. DESIGN:   Descriptive epidemiology study...
November 20, 2017: Journal of Athletic Training
https://www.readbyqxmd.com/read/29153813/surgical-stabilization-of-rib-fractures-in-a-6-year-old-child-after-blunt-trauma
#5
Zaid M Abdelsattar, Michael B Ishitani, Brian D Kim
When identified, rib fractures in children are associated with high-energy trauma, nonaccidental trauma, or both. Traditionally, the optimal management of rib fractures in children is supportive care. In this case report, we present a 6-year-old boy who underwent surgical rib fixation for multiple displaced and comminuted rib fractures after being stepped on by a horse.
December 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29153110/increased-adrenocortical-response-to-adrenocorticotropic-hormone-acth-in-sport-horses-with-equine-glandular-gastric-disease-eggd
#6
M D Scheidegger, V Gerber, R M Bruckmaier, J H van der Kolk, D Burger, A Ramseyer
This study tested the hypothesis that adrenocortical function would be altered in horses with equine gastric ulcer syndrome (EGUS). Twenty-six sport horses competing at national or international levels in eventing (n=15) or endurance (n=11) were subjected to a gastroscopic examination and an adrenocorticotropic hormone (ACTH) stimulation test. Salivary cortisol concentrations were measured before (baseline) and after (30, 60, 90, 120 and 150min) IV ACTH injection (1μg/kg bodyweight). Within EGUS, two distinct diseases, equine squamous gastric disease (ESGD) and equine glandular gastric disease (EGGD), can be distinguished...
October 2017: Veterinary Journal
https://www.readbyqxmd.com/read/29152319/the-effect-of-sodium-bicarbonate-and-validation-of-beckman-coulter-au680-analyzers-for-measuring-total-carbon-dioxide-tco2-concentrations-in-horse-serum
#7
Levent Dirikolu, Pamela Waller, Mona Landry Waguespack, Frank Michael Andrews, Michael Layne Keowen, Stephen David Gaunt
This study evaluated the usage of Beckman Coulter AU680 analyzers for measurement of TCO 2 in horse serum, and the effect of sodium bicarbonate administrations on serum TCO 2 levels in resting horses. Treatment of horses with sodium bicarbonate did not result in any adverse events. Mean TCO 2 concentration was significantly higher from 1 to 8 h in the sodium bicarbonate-treated horses compared to the untreated controls. Within an hour, administration of sodium bicarbonate increased the TCO 2 level from 31...
November 2017: Veterinary Medicine and Science
https://www.readbyqxmd.com/read/29152317/bone-marrow-derived-multipotent-mesenchymal-stromal-cells-from-horses-after-euthanasia
#8
Carmen Schröck, Carina Eydt, Florian Geburek, Lena Kaiser, Felicitas Päbst, Janina Burk, Christiane Pfarrer, Carsten Staszyk
Allogeneic equine multipotent mesenchymal stromal cells (eMSCs) have been proposed for use in regenerative therapies in veterinary medicine. A source of allogeneic eMSCs might be the bone marrow from euthanized horses. The purpose of this study was to compare in vitro characteristics of equine bone marrow derived eMSC (eBM-MSCs) from euthanized horses (eut-MSCs) and from narcotized horses (nar-MSCs). Eut-MSCs and nar-MSCs showed typical eMSC marker profiles (positive: CD44, CD90; negative: CD11a/CD18 and MHCII) and possessed tri-lineage differentiation characteristics...
November 2017: Veterinary Medicine and Science
https://www.readbyqxmd.com/read/29152314/seroprevalence-of-west-nile-virus-in-horses-in-different-moroccan-regions
#9
Abdennasser Benjelloun, Mehdi El Harrak, Paolo Calistri, Chafiqa Loutfi, Hafsa Kabbaj, Annamaria Conte, Carla Ippoliti, Maria Luisa Danzetta, Bouchra Belkadi
West Nile virus-associated disease is one of the most widespread vector-borne diseases in the world. In Morocco, the first cases were reported in horses in 1996 and the disease re-emerged in 2003 and in 2010. The objective of this work was to study the epidemiological situation of WNV-associated infection in Morocco, by quantifying the seroprevalence of anti-WNV IgM and IgG antibodies in horses in different bioclimatic regions-zones of Morocco in 2011. During the months of May, June and July 2011, 840 serum samples were collected from horses in four regions characterized by different environmental and climatic features such as altitude, temperature and precipitation...
November 2017: Veterinary Medicine and Science
https://www.readbyqxmd.com/read/29152313/pharmacokinetic-profile-and-partitioning-in-red-blood-cells-of-romifidine-after-single-intravenous-administration-in-the-horse
#10
Noemi Romagnoli, Khaled M Al-Qudah, Sara Armorini, Carlotta Lambertini, Anna Zaghini, Alessandro Spadari, Paola Roncada
The aims of this study were to assess the plasma concentrations of romifidine in horses after intravenous injection, to evaluate the red blood cell (RBC) partitioning of the anaesthetic drug, and to improve knowledge regarding its sedative effect in horses describing the pharmacokinetic model. Eight adult Standardbred horses received a single bolus of romifidine at a dosage of 100 μg/kg. Blood samples (5 mL) were collected immediately before romifidine administration (t0), and at 2, 5, 10, 15, 20, 25, 30, 40, 50, 60, 75, 90, 105, 120, 150 and 180 min after injection...
November 2017: Veterinary Medicine and Science
https://www.readbyqxmd.com/read/29152277/uveal-myxoid-leiomyosarcoma-in-a-horse
#11
Siv Grosås, Liv Østevik, Tobias Revold, Nina Ottesen, Ernst-Otto Ropstad
A uveal leiomyosarcoma of a horse is reported. There are few published reports of intraocular tumors in horses. Intraocular tumors challenge animal welfare by causing uveitis, glaucoma, and loss of vision. Knowledge regarding treatment of intraocular tumors with globe preservation is sparse, and further investigations on this topic are required.
November 2017: Clinical Case Reports
https://www.readbyqxmd.com/read/29151450/identification-and-expression-analysis-of-cdna-encoding-insulin-like-growth-factor-2-in-horses
#12
Kohta Kikuchi, Keisuke Sasaki, Hiroki Akizawa, Hayato Tsukahara, Hanako Bai, Masashi Takahashi, Yasuo Nambo, Hiroshi Hata, Manabu Kawahara
Insulin-like growth factor 2 (IGF2) is responsible for a broad range of physiological processes during fetal development and adulthood, but genomic analyses of IGF2 containing the 5'- and 3'-untranslated regions (UTRs) in equines have been limited. In this study, we characterized the IGF2 mRNA containing the UTRs, and determined its expression pattern in the fetal tissues of horses. The complete equine IGF2 mRNA sequence harboring another exon approximately 2.8 kb upstream from the canonical transcription start site was identified as a new transcript variant...
November 17, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29151087/ganser-syndrome
#13
Sebastian Dieguez
Ganser's syndrome is a rare and controversial condition, whose main and most striking feature is the production of approximate answers (or near misses) to very simple questions. For instance, asked how many legs a horse has, Ganser patients will reply "5", and answers to plain arithmetic questions will likewise be wrong, but only slightly off the mark (e.g., 2 + 2 = 3). This symptom was originally described by Sigbert Ganser in 1897 in prisoners on remand and labeled Vorbeigehen ("to pass by"), although the term Vorbeireden ("to talk beside the point") is also frequently used...
2018: Frontiers of Neurology and Neuroscience
https://www.readbyqxmd.com/read/29150578/from-strangles-to-sore-throats-gene-identification-technique-could-help-horses-and-people
#14
(no author information available yet)
No abstract text is available yet for this article.
November 18, 2017: Veterinary Record
https://www.readbyqxmd.com/read/29149701/risk-factor-analysis-of-equine-strongyle-resistance-to-anthelmintics
#15
G Sallé, J Cortet, I Bois, C Dubès, Q Guyot-Sionest, C Larrieu, V Landrin, G Majorel, S Wittreck, E Woringer, A Couroucé, J Guillot, P Jacquiet, F Guégnard, A Blanchard, A Leblond
Intestinal strongyles are the most problematic endoparasites of equids as a result of their wide distribution and the spread of resistant isolates throughout the world. While abundant literature can be found on the extent of anthelmintic resistance across continents, empirical knowledge about associated risk factors is missing. This study brought together results from anthelmintic efficacy testing and risk factor analysis to provide evidence-based guidelines in the field. It involved 688 horses from 39 French horse farms and riding schools to both estimate Faecal Egg Count Reduction (FECR) after anthelmintic treatment and to interview farm and riding school managers about their practices...
November 2, 2017: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/29149628/multiplex-taqman-locked-nucleic-acid-real-time-pcr-for-the-differential-identification-of-various-meat-and-meat-products
#16
Rusu Xu, Shuang Wei, Guangbiao Zhou, Jiao Ren, Zhongyong Liu, Shuze Tang, Peter C K Cheung, Xiyang Wu
Meat adulteration incidents have been reported frequently over the past few years, and the corresponding traceability issues attracted much more attention due to the customer's demands and administration's responsibility. Therefore, it is important to develop high-throughput and rapid detection methods to identify the specific sources from meat samples. In this study, a multiplex TaqMan locked nucleic acid real-time polymerase chain reaction assay (MLNA-RT-PCR) was developed to simultaneously detect multiple meat sources (duck, pork, beef and chicken)...
November 14, 2017: Meat Science
https://www.readbyqxmd.com/read/29148375/west-nile-virus-lineage-2-in-horses-and-other-animals-with-neurologic-disease-south-africa-2008-2015
#17
Marietjie Venter, Marthi Pretorius, James A Fuller, Elizabeth Botha, Mpho Rakgotho, Voula Stivaktas, Camilla Weyer, Marco Romito, June Williams
During 2008-2015 in South Africa, we conducted West Nile virus surveillance in 1,407 animals with neurologic disease and identified mostly lineage 2 cases in horses (7.4%, 79/1,069), livestock (1.5%, 2/132), and wildlife (0.5%, 1/206); 35% were fatal. Geographic correlation of horse cases with seropositive veterinarians suggests disease in horses can predict risk in humans.
December 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29148268/development-and-validation-of-a-uhplc-hrms-ms-quantification-method-for-hypoglycin-a-and-mcpa-carnitine-in-horse-serum-in-cases-of-atypical-myopathy
#18
Wiebke Rudolph, Daniela Remane, Dirk K Wissenbach, Carmen Klein, Dirk Barnewitz, Frank T Peters
Atypical myopathy (AM) is a fatal disease in horses presumably caused by hypoglycin A (HGA) from ingested maple seeds and its active metabolite methylenecyclopropyl-acetic-acid (MCPA). The aim of this study was the development and validation of a rapid and simple assay for HGA and MCPA-carnitine in horse serum and its application to authentic samples. Identification and quantification were carried out by high resolution LC-MS/MS with fullscan/data-dependent MS(2) . Chromatographic separation was performed by isocratic elution on a HILIC column (100 x 2...
November 17, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29148168/phenylbutazone-induces-equine-glandular-gastric-disease-without-decreasing-prostaglandin-e2-concentrations
#19
S K Pedersen, A E Cribb, E K Read, D French, H E Banse
In equids, phenylbutazone at high doses induces gastric disease, primarily in the glandular portion of the stomach. However, the mechanism of nonsteroidal anti-inflammatory drug (NSAID)-induced gastric disease in horses has yet to be determined. While phenylbutazone-associated ulceration is often attributed to a decrease in basal gastric prostaglandins, this has not been demonstrated in the horse. Twelve horses were randomly assigned to treatment (n = 6; 4.4 mg/kg phenylbutazone PO in 20 ml molasses q 12 hr for 7 days) or placebo (n = 6; 20 ml molasses PO q 12 hr for 7 days) groups...
November 16, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29147931/horses-for-courses-moving-india-towards-universal-health-coverage-through-targeted-policy-design
#20
Dayashankar Maurya, Altaf Virani, S Rajasulochana
The debate on how India's health system should move towards universal health coverage was (meant to be) put to rest by the recent National Health Policy 2017. However, the new policy is silent about tackling bottlenecks mentioned in the said policy proposal. It aims to provide universal access to free primary care by strengthening the public system, and to secondary and tertiary care through strategic purchasing from the private sector, to overcome deficiencies in public provisioning in the short run. Yet, in doing so, it ignores critical factors needed to replicate successful models of public healthcare delivery from certain states that it hopes to emulate...
November 16, 2017: Applied Health Economics and Health Policy
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