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https://www.readbyqxmd.com/read/28651344/uterine-responses-to-early-pre-attachment-embryos-in-the-domestic-dog-and-comparisons-with-other-domestic-animal-species
#1
Felix R Graubner, Aykut Gram, Ewa Kautz, Stefan Bauersachs, Selim Aslan, Ali R Agaoglu, Alois Boos, Mariusz P Kowalewski
In the dog there is no luteolysis in the absence of pregnancy. Thus, this species lacks any anti-luteolytic endocrine signal as found in other species that modulate uterine function during the critical period of pregnancy establishment. Nevertheless, in the dog an embryo-maternal communication must occur in order to prevent rejection of embryos. Based on this hypothesis, we performed microarray analysis of canine uterine samples collected during pre-attachment phase (days 10-12) and in corresponding non-pregnant controls, in order to elucidate the embryo attachment signal...
June 23, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28650243/antigenicity-of-mesenchymal-stem-cells-in-an-inflamed-joint-environment
#2
Jacqueline A Hill, Jennifer M Cassano, Margaret B Goodale, Lisa A Fortier
OBJECTIVE To determine whether major histocompatability complex (MHC) class II expression in equine mesenchymal stem cells (MSCs) changes with exposure to a proinflammatory environment reflective of an inflamed joint. SAMPLE Cryopreserved bone marrow-derived MSCs from 12 horses and cartilage and synovium samples from 1 horse euthanized for reasons other than lameness. PROCEDURES In part 1 of a 3-part study, the suitability of a quantitative reverse transcriptase PCR (qRT-PCR) assay for measurement of MHC class II expression in MSCs following stimulation with interferon (IFN)-γ was assessed...
July 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28650242/evaluation-of-a-telemetric-gastrointestinal-pill-for-continuous-monitoring-of-gastrointestinal-temperature-in-horses-at-rest-and-during-exercise
#3
Elisabeth-Lidwien J M M Verdegaal, Catherine Delesalle, Charles G B Caraguel, Louise E Folwell, Todd J McWhorter, Gordon S Howarth, Samantha H Franklin
OBJECTIVE To evaluate use of a telemetric gastrointestinal (GI) pill to continuously monitor GI temperature in horses at rest and during exercise and to compare time profiles of GI temperature and rectal temperature. ANIMALS 8 Standardbred horses. PROCEDURES Accuracy and precision of the GI pill and a rectal probe were determined in vitro by comparing temperature measurements with values obtained by a certified resistance temperature detector (RTD) in water baths at various temperatures (37°, 39°, and 41°C)...
July 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28650237/racing-performance-of-standardbred-trotting-horses-undergoing-surgery-of-the-carpal-flexor-sheath-and-age-and-sex-matched-control-horses
#4
James L Carmalt, Bengt C Johansson, Sandra M Zetterström, Rebecca C McOnie
OBJECTIVE To determine factors affecting race speed in Swedish Standardbred horses undergoing surgery of the carpal flexor sheath (CFS), to investigate whether preoperative racing speed was associated with specific intraoperative findings and whether horses returned to racing, and to compare the performance of horses undergoing surgery of the CFS with that of age- and sex-matched control horses. ANIMALS 149 Swedish Standardbred trotters undergoing surgery of the CFS and 274 age- and sex-matched control horses...
July 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28650233/evaluation-of-clinical-and-tenoscopic-findings-in-the-carpal-flexor-sheath-of-horses
#5
Sandra M Zetterström, Bengt C Johansson, James L Carmalt
OBJECTIVE To evaluate clinical and tenoscopic findings in a large group of horses undergoing surgery of the carpal flexor sheath (CFS) and determine whether any of the presurgical clinical signs were associated with tenoscopic findings. ANIMALS 242 horses that had undergone diagnostic and therapeutic tenoscopy of the CFS because of aseptic tenosynovitis. PROCEDURES Medical and tenoscopic video records (when available) of 242 horses undergoing tenoscopy of the CFS at a single equine clinic between January 2005 and June 2014 were reviewed...
July 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28650085/immunogenicity-of-plant-produced-african-horse-sickness-virus-like-particles-implications-for-a-novel-vaccine
#6
Susan J Dennis, Ann E Meyers, Alan J Guthrie, Inga I Hitzeroth, Edward P Rybicki
African horse sickness (AHS) is a debilitating and often fatal viral disease affecting horses in much of Africa, caused by the dsRNA orbivirus African horse sickness virus (AHSV). Vaccination remains the single most effective weapon in combatting AHS, as there is no treatment for the disease apart from good animal husbandry. However, the only commercially available vaccine is a live attenuated version of the virus (LAV). The threat of outbreaks of the disease outside its endemic region and the fact that the LAV is not licensed for use elsewhere in the world, have spurred attempts to develop an alternative safer, yet cost-effective recombinant vaccine...
June 26, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28649361/control-of-lice-infestation-in-horses-using-a-10-mg-ml-deltamethrin-topical-application
#7
Eloy Castilla-Castaño, Alessandro Vischi, Christelle Navarro, Line Alice Lecru, Claudia Ribeiro, Sophie Pradier, Marie-Christine Cadiergues
BACKGROUND: Two open-controlled studies evaluated the tolerance and the efficacy of a 10 mg/mL deltamethrin-based pour-on solution (Deltanil®; Virbac, France) in treating (study 1) and preventing (study 2) natural Damalinia equi infestations in horses. In study 1, seven adult horses received 10 mL of the solution from mane to tail head on day 0 (D0). Four adult horses, living separately, served as non-treated controls. All were naturally infected. Lice burden was recorded by counting the number of live parasites, bilaterally, over seven anatomic regions...
2017: Irish Veterinary Journal
https://www.readbyqxmd.com/read/28648869/long-term-cytokine-and-growth-factor-release-from-equine-platelet-rich-fibrin-clots-obtained-with-two-different-centrifugation-protocols
#8
Román F Jiménez-Aristizabal, Catalina López, María E Álvarez, Carlos Giraldo, Marta Prades, Jorge U Carmona
OBJECTIVES: To compare the temporal release (over three weeks) of tumor necrosis factor alpha (TNF-α), interleukin 4 (IL-4), IL-1 receptor antagonist (IL-1ra), platelet-derived growth factor BB (PDGF-BB) and transforming growth factor beta-1 (TGF-β1) from two platelet-rich fibrin (PRF) preparations from equine blood obtained at either 240g/8min or 416g/10min. METHODS: Whole blood from 10 horses was used to obtain PRF clots by two different centrifugation protocols...
June 22, 2017: Cytokine
https://www.readbyqxmd.com/read/28648789/detection-of-west-nile-virus-and-other-common-equine-viruses-in-three-locations-from-the-leeward-islands-west-indies
#9
Pompei Bolfa, Isaac Jeon, Amanda Loftis, Teresa Leslie, Silvia Marchi, Fortune Sithole, Cecile Beck, Sylvie Lecollinet, Stephan Zientara, Aymeric Hans, Charles J Issel
Equines in the West Indies are used for recreational purposes, tourism industry, racing and agriculture or can be found in feral populations. Little is known in the Caribbean basin about the prevalence of some major equine infectious diseases, some with zoonotic potential, listed as reportable by the OIE. Our objective was to study the prevalence of antibodies for West Nile Virus (WNV), Equine Herpes Virus-1 and 4 (EHV-1 and EHV-4), Equine Influenza (EI), Equine Viral Arteritis (EVA) and Equine Infectious Anemia Virus (EIAV) using a retrospective serological convenience study...
June 22, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28648787/online-turbulent-flow-extraction-coupled-with-liquid-chromatography-tandem-mass-spectrometry-for-high-throughput-screening-of-anabolic-steroids-in-horse-urine
#10
Hyun Du Shin, Joon Hyuk Suh, Junghyun Kim, Hyun-Deok Cho, Su Duk Lee, Kwan Seok Han, Yu Wang, Sang Beom Han
A high throughput method for simultaneous screening of anabolic steroids and their metabolites (4-esterendione, trenbolone, boldenone, oxandrolone, nandrolone, methandrostenolone, testosterone, 1-androstendione, ethisterone, normethandrolone, methyltestosterone, 16β-Hydroxystanozolol, epitestosterone, bolasterone, norethandrolone, danazol, stanozolol and androstadienone) in equine urine by online turbulent flow extraction coupled with liquid chromatography-tandem mass spectrometry was developed. The use of turbulent flow chromatography could simplify pretreatment of horse urine, which has complex matrices as well as high viscosity...
June 19, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28647600/the-in-vitro-biocompatibility-of-d-raffinose-modified-chitosan-two-dimensional-and-three-dimensional-systems-for-culturing-of-horse-articular-chondrocytes
#11
Elena De Angelis, Francesca Ravanetti, Paolo Martelli, Antonio Cacchioli, Ana Ivanovska, Attilio Corradi, Sonia Nasi, Annalisa Bianchera, Benedetta Passeri, Elena Canelli, Ruggero Bettini, Paolo Borghetti
The present study investigated the biocompatibility of chitosan films and scaffolds modified with d-(+)raffinose and their capability to support the growth and maintenance of the differentiation of articular chondrocytes in vitro. Primary equine articular chondrocytes were cultured on films and scaffolds of modified d-(+) raffinose chitosan. Their behavior was compared to that of chondrocytes grown in conventional bi- and three-dimensional culture systems, such as micromasses and alginate beads. Chitosan films maintained the phenotype of differentiated chondrocytes (typical round morphology) and sustained the synthesis of cartilaginous extracellular matrix (ECM), even at 4weeks of culture...
June 15, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28646050/fve-and-feeva-urge-european-commission-to-allow-bute-to-be-used-in-horses
#12
(no author information available yet)
No abstract text is available yet for this article.
June 24, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28645680/collection-processing-and-freezing-of-equine-bone-marrow-cells
#13
Fernanda Z Bastos, Fernanda C M Barussi, Thasla F Santi, Bianca P Vieira, Alexandra C Senegaglia, Fernanda F Cruz, Pedro V Michelotto
There is no consensus on aspects of equine bone marrow collection and processing. The study aimed to describe the collection of large volumes of bone marrow from horses of advanced age, with emphasis on bone marrow mononuclear cells (BMMCs) recovery and viability after cryopreservation. Fourteen horses, aged 3-24 years, were divided into three experiments. E1 studied the feasibility of collecting 200 mL from the sternums of horses of advanced age; E2 examined the number of cells obtained from the first and last syringe of each puncture; and E3 investigated the influence of heparin concentration on the prevention of cell aggregation, and cell viability after freezing in liquid nitrogen...
June 20, 2017: Cryobiology
https://www.readbyqxmd.com/read/28645313/molecular-investigation-and-phylogeny-of-anaplasmataceae-species-infecting-domestic-animals-and-ticks-in-corsica-france
#14
Mustapha Dahmani, Bernard Davoust, Djamel Tahir, Didier Raoult, Florence Fenollar, Oleg Mediannikov
BACKGROUNDS: Corsica is a French island situated in the Mediterranean Sea. The island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. The family Anaplasmataceae is a member of the order Rickettsiales; it includes the genera Anaplasma, Ehrlichia, Neorickettsia and Wolbachia. Anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera Anaplasma and Ehrlichia...
June 23, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28643340/trans-endoscopic-diode-laser-fenestration-of-equine-conchae-via-contralateral-nostril-approach
#15
Filip Kološ, Štěpán Bodeček, Zdeněk Žert
OBJECTIVE: To describe and report preliminary outcomes of a contralateral trans-nasal endoscopic laser fenestration of the conchae for endoscopic examination and treatment of paranasal sinuses in horses. STUDY DESIGN: Cadaveric experimental and prospective clinical study. ANIMALS: Normal cadaveric equine heads (n = 7) and equine patients (horses n = 7, donkey n = 1) diagnosed with sinusitis. METHODS: Ex vivo: a video-endoscope containing a diode laser fiber in the working canal was passed through the nostril and retroflexed in the nasopharynx toward the contralateral conchae...
June 23, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/28642343/differential-distribution-of-vapa-positive-rhodococcus-equi-in-affected-and-unaffected-horse-breeding-farms
#16
S Petry, C Sévin, M A Fleury, F Duquesne, N Foucher, C Laugier, M Henry-Amar, J Tapprest
No abstract text is available yet for this article.
June 22, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28640600/horse-liver-alcohol-dehydrogenase-zinc-coordination-and-catalysis
#17
Bryce V Plapp, Baskar Raj Savarimuthu, Daniel J Ferraro, Jon K Rubach, Eric N Brown, Subramanian Ramaswamy
: During catalysis by liver alcohol dehydrogenase (ADH), a water bound to the catalytic zinc is replaced by the oxygen of the substrates. The mechanism may involve a pentacoordinated zinc or a double displacement reaction with participation by a nearby glutamate residue, as suggested by studies of human ADH3, yeast ADH1 and some other tetrameric ADHs. Zinc coordination and participation of water in the enzyme mechanism were investigated by X-ray crystallography. Apoenzyme and its complex with adenosine 5'-diphosphoribose have an open protein conformation with the catalytic zinc in one position, tetracoordinated by Cys-46, His-67, Cys-174 and a water molecule...
June 22, 2017: Biochemistry
https://www.readbyqxmd.com/read/28640590/chlorin-e6-functionalized-theranostic-multistage-nanovectors-transported-by-stem-cells-for-effective-photodynamic-therapy
#18
Simo Näkki, Jonathan Martinez, Michael Evangelopoulos, Wujun Xu, Vesa-Pekka Lehto, Ennio Tasciotti
Approaches to achieve site-specific and targeted delivery that provide an effective solution to reduce negative, off target side-effects are urgently needed for cancer therapy. Here, we utilized a Trojan horse-like strategy to carry photosensitizer Chlorin e6 conjugated porous silicon multistage nanovectors with tumor homing mesenchymal stem cells for targeted photodynamic therapy and diagnosis. The inherent versatility of multistage nanovectors permitted the conjugation of photosensitizers to enable precise cell death induction (60%) upon photodynamic therapy, while simultaneously retaining the loading capacity to load various payloads, such as antitumor drugs and diagnostic nanoparticles...
June 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28638626/effects-of-liposomal-curcumin-on-five-opportunistic-bacterial-strains-found-in-the-equine-hindgut-preliminary-study
#19
S D Bland, E B Venable, J L McPherson, R L Atkinson
BACKGROUND: The horse intestinal tract is sensitive and contains a highly complex microbial population. A shift in the microbial population can lead to various issues such as inflammation and colic. The use of nutraceuticals in the equine industry is on the rise and curcumin is thought to possess antimicrobial properties that may help to minimize the proliferation of opportunistic bacteria. METHODS: Four cecally-cannulated horses were utilized to determine the optimal dose of liposomal-curcumin (LIPC) on reducing Streptococcus bovis/equinus complex (SBEC), Escherichia coli K-12, Escherichia coli general, Clostridium difficile, and Clostridium perfringens in the equine hindgut without adversely affecting cecal characteristics...
2017: Journal of Animal Science and Technology
https://www.readbyqxmd.com/read/28637457/descriptive-and-network-analyses-of-the-equine-contact-network-at-an-equestrian-show-in-ontario-canada-and-implications-for-disease-spread
#20
Kelsey L Spence, Terri L O'Sullivan, Zvonimir Poljak, Amy L Greer
BACKGROUND: Identifying the contact structure within a population of horses attending a competition is an important element towards understanding the potential for the spread of equine pathogens as the horses subsequently travel from location to location. However, there is limited information in Ontario, Canada to quantify contact patterns of horses. The objective of this study was to describe the network of potential contacts associated with an equestrian show to determine how this network structure may influence potential disease transmission...
June 21, 2017: BMC Veterinary Research
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