keyword
MENU ▼
Read by QxMD icon Read
search

short tandem repeats

keyword
https://www.readbyqxmd.com/read/29353342/genetic-analysis-of-17-y-str-loci-from-1026-individuals-of-han-populations-in-jilin-province-northeast-china
#1
Wen-Qing Chen, Zhen Feng, Wei Jin, Yi-Jun Zhang, Qiang Wang, Pei Li, Shi-Lin Li, Lan-Hai Wei, Gang Liu
In this study, 17 Y-chromosomal short tandem repeats (Y-STRs) were analyzed in 1026 male individuals of Han populations in Changchun City, Jilin Province, Northeast China. The haplotype diversity is 0.99892. The Changchun Han population is close to most Han populations and different from most other minority populations of China. Additionally, the Changchun Han show more affiliations with Han populations in North and Northeast China. These data provide a reference for the Y-STR database in Jilin Province, and they may be valuable for population genetic analysis...
January 20, 2018: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/29352241/short-tandem-repeat-analysis-after-whole-genome-amplification-of-single-b-lymphoblastoid-cells
#2
Lieselot Deleye, Ann-Sophie Vander Plaetsen, Jana Weymaere, Dieter Deforce, Filip Van Nieuwerburgh
To allow multiple genetic analyses on a single cell, whole genome amplification (WGA) is required. Unfortunately, studies comparing different WGA methods for downstream human identification Short Tandem Repeat (STR) analysis remain absent. Therefore, the aim of this work was to assess the performance of four commercially available WGA kits for downstream human identification STR profiling on a B-lymphoblastoid cell line. The performance was assessed using an input of one or three micromanipulated cells. REPLI-g showed a very low dropout rate, as it was the only WGA method in this study that could provide a complete STR profile in some of its samples...
January 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29351317/hafts-are-novel-lncrna-transcripts-from-aflatoxin-exposure
#3
B Alex Merrick, Justin S Chang, Dhiral P Phadke, Meredith A Bostrom, Ruchir R Shah, Xinguo Wang, Oksana Gordon, Garron M Wright
The transcriptome can reveal insights into precancer biology. We recently conducted RNA-Seq analysis on liver RNA from male rats exposed to the carcinogen, aflatoxin B1 (AFB1), for 90 days prior to liver tumor onset. Among >1,000 differentially expressed transcripts, several novel, unannotated Cufflinks-assembled transcripts, or HAfTs (Hepatic Aflatoxin Transcripts) were found. We hypothesized PCR-cloning and RACE (rapid amplification of cDNA ends) could further HAfT identification. Sanger data was obtained for 6 transcripts by PCR and 16 transcripts by 5'- and 3'-RACE...
2018: PloS One
https://www.readbyqxmd.com/read/29350270/genetic-data-for-26-autosomal-str-markers-from-brazilian-population
#4
Tamiris Fátima Correia Pereira, Marcelo Malaghini, João Carlos Maciel Magalhães, Rodrigo Moura-Neto, Vanessa Santos Sotomaior
The allelic frequency distributions and statistical forensic parameters of 26 mini short tandem repeat (mini-STR) loci in a sample of 1575 unrelated individuals from five different Brazilian regions were obtained. All the analyzed loci showed great diversity and were highly informative. The results were compared with those of the US Caucasian, African American, and Hispanic population studies. This study aimed to contribute to forensic analysis for human identification and inference of the evidential value in familial bond tests...
January 19, 2018: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/29335550/the-peg-responding-desiccome-of-the-alder-microsymbiont-frankia-alni
#5
Kais Ghedira, Emna Harigua-Souiai, Cherif Ben Hamda, Pascale Fournier, Petar Pujic, Sihem Guesmi, Ikram Guizani, Guylaine Miotello, Jean Armengaud, Philippe Normand, Haïtham Sghaier
Actinorhizal plants are ecologically and economically important. Symbiosis with nitrogen-fixing bacteria allows these woody dicotyledonous plants to colonise soils under nitrogen deficiency, water-stress or other extreme conditions. However, proteins involved in xerotolerance of symbiotic microorganisms have yet to be identified. Here we characterise the polyethylene glycol (PEG)-responding desiccome from the most geographically widespread Gram-positive nitrogen-fixing plant symbiont, Frankia alni, by next-generation proteomics, taking advantage of a Q-Exactive HF tandem mass spectrometer equipped with an ultra-high-field Orbitrap analyser...
January 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29332164/skewing-of-the-genetic-architecture-at-the-zmym3-human-specific-5-utr-short-tandem-repeat-in-schizophrenia
#6
F Alizadeh, A Bozorgmehr, J Tavakkoly-Bazzaz, M Ohadi
Differential expansion of a number of human short tandem repeats (STRs) at the critical core promoter and 5' untranslated region (UTR) support the hypothesis that at least some of these STRs may provide a selective advantage in human evolution. Following a genome-wide screen of all human protein-coding gene 5' UTRs based on the Ensembl database ( http://www.ensembl.org ), we previously reported that the longest STR in this interval is a (GA)32, which belongs to the X-linked zinc finger MYM-type containing 3 (ZMYM3) gene...
January 13, 2018: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/29318123/genetic-linkage-analysis-of-dfnb4-dfnb28-dfnb93-loci-in-autosomal-recessive-non-syndromic-hearing-loss-evidence-for-digenic-inheritance-in-gjb2-and-gjb3-mutations
#7
Marzieh Naseri, Masoud Akbarzadehlaleh, Marjan Masoudi, Najmeh Ahangari, Ali Akbar Poursadegh Zonouzi, Ahmad Poursadegh Zonouzi, Leila Shams, Azim Nejatizadeh
Background: Autosomal recessive non-syndromic hearing loss (ARNSHL) a most frequent hereditary type of hearing impairment, exhibit tremendous genetic heterogeneity. We aimed to determine the contribution of three common DFNB loci (DFNB4, DFNB28, and DFNB93), and mutation analysis of Gap Junction Beta-2 gene (GJB2) and GJB3 genes in ARNSHL subjects in southern Iran. Methods: Thirty-six large ARNSHL pedigrees (167 individuals) with at least two affected subjects (72 patients) were included in this descriptive study from Hormozgan Province of Iran, during 2014 - 2015...
January 2018: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29305387/simple-and-complex-centromeric-satellites-in-drosophila-sibling-species
#8
Paul Talbert, Sivakanthan Kasinathan, Steven Henikoff
Centromeres are the chromosomal sites of assembly for kinetochores, the protein complexes that attach to spindle fibers and mediate separation of chromosomes to daughter cells in mitosis and meiosis. In most multicellular organisms, centromeres comprise a single specific family of tandem repeats, often 100-400 bp in length, found on every chromosome, typically in one location within heterochromatin. Drosophila melanogaster is unusual in that the heterochromatin contains many families of mostly short (5-12 bp) tandem repeats, none of which appear to be present at all centromeres, and none of which are found only at centromeres...
January 5, 2018: Genetics
https://www.readbyqxmd.com/read/29305242/allelic-and-haplotype-diversity-of-12-x-strs-in-the-united-arab-emirates
#9
Mohamed A Almarri, Reem A Lootah
X-chromosomal short tandem repeats (X-STRs) are used to complement autosomal and Y-STRs in complex kinship testing, and can be of potential value when determining trace female DNA in male background. We analyzed extracted and directly-amplified samples of 501 males from the United Arab Emirates and report the forensic statistical parameters of 12 X-STRs and haplotypes of the four previously defined linkage groups using the Investigator Argus X-12 QS kit. Combined PDM, PDF, MEC Desmarais Trio and MEC Desmarais Duo based on allele (and haplotype) frequencies were 0...
December 29, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29301114/neither-femur-nor-tooth-petrous-bone-for-identifying-archaeological-bone-samples-via-forensic-approach
#10
Elena Pilli, Stefania Vai, Marina Grazia Caruso, Giancarlo D'Errico, Andrea Berti, David Caramelli
One of the major challenges of molecular biology in anthropological analysis is the identification via DNA typing of bone or teeth samples that can be collected from archaeological site in order to investigate kinship relationships. Due to the difficulties of isolating and analysing DNA from such samples, several efforts have been made to solve these problems, but less work has been conducted to identify the proper type of bone samples for the DNA analysis. Therefore, following the promising results obtained from the DNA analysis of petrous bones by different groups of researchers, for the first time, here we investigated the possibility of using petrous bones as skeletal elements useful for short tandem repeat (STR) typing via capillary electrophoresis technique in ancient bone samples...
December 24, 2017: Forensic Science International
https://www.readbyqxmd.com/read/29300948/the-effects-of-transcription-and-recombination-on-mutational-dynamics-of-short-tandem-repeats
#11
Monika Zavodna, Andrew Bagshaw, Rudiger Brauning, Neil J Gemmell
Short tandem repeats (STR) are ubiquitous components of the genomic architecture of most living organisms. Recent work has highlighted the widespread functional significance of such repeats, particularly around gene regulation, but the mutational processes underlying the evolution of these highly abundant and highly variable sequences are not fully understood. Traditional models assume that strand misalignment during replication is the predominant mechanism, but empirical data suggest the involvement of other processes including recombination and transcription...
December 29, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29285029/establishment-and-characterization-of-primary-cultures-from-iranian-oral-squamous-cell-carcinoma-patients-by-enzymatic-method-and-explant-culture
#12
Meysam Ganjibakhsh, Pouyan Aminishakib, Parvaneh Farzaneh, Abbas Karimi, Seyed Abolhassan Shahzadeh Fazeli, Moones Rajabi, Ahmad Nasimian, Fereshteh Baghai Naini, Hedieh Rahmati, Neda Sadat Gohari, Nazanin Mohebali, Masoumeh Asadi, Zahra Elyasi Gorji, Mehrnaz Izadpanah, Shiva Mohamadi Moghanjoghi, Sepideh Ashouri
Objectives: Oral Squamous Cell Carcinoma (OSCC) is the most frequent oral cancer worldwide. It is known as the eighth most common cancer in men and as the fifth most common cancer in women. Cytogenetic and biochemical studies in recent decades have emphasized the necessity of providing an appropriate tool for such researches. Cancer cell culture is a useful tool for investigations on biochemical, genetic, molecular and immunological characteristics of different cancers, including oral cancer...
July 2017: Journal of Dentistry of Tehran University of Medical Sciences
https://www.readbyqxmd.com/read/29283004/elimination-of-contaminating-amplified-short-tandem-repeat-products-by-autoclaving-and-ultraviolet-irradiation
#13
Jun Ohta, Atsunori Tanaka
DNA contamination can result in false interpretation of short tandem repeat (STR) DNA typing. Proper decontamination is particularly required in forensic DNA laboratories where probative value of the evidence may be affected. The aim of this study was to establish an effective DNA decontamination procedure for amplified STR products focusing on laboratory-related contamination. We verified the effectiveness of thermally and temporally extended autoclaving and ultraviolet irradiation for the elimination of contaminating amplified STR products...
January 1, 2017: Medicine, Science, and the Law
https://www.readbyqxmd.com/read/29282760/development-and-implementation-of-a-novel-panel-consisting-20-markers-for-the-detection-of-genetic-causes-of-male-infertility
#14
S Bahrami Zadegan, S Dabbagh Bagheri, A Joudaki, M H Samiee Aref, A H Saeidian, M Abiri, S Zeinali
Azoospermia factor (AZF) genes are involved in spermatogenesis. Deletions in the region of these genes have been recognised as a major genetic cause of infertility due to defects in spermatogenesis. Klinefelter syndrome (KS) is the other main cause of male infertility. This study was performed to establish a novel method for the detection of genetic causes of infertility in males and also to investigate the prevalence, extent and position of Y chromosome microdeletions in Iranian infertile men. We developed a newly designed panel of fluorescent multiplex-PCR method to amplify 20 markers (15 sequence-tagged sites (STSs) markers which are placed in the Y chromosome AZF region, 2 short tandem repeats (STRs) and 3 segmental duplications (SDs))...
December 28, 2017: Andrologia
https://www.readbyqxmd.com/read/29275088/genetic-analysis-of-southern-brazil-subjects-using-the-powerseq%C3%A2-auto-y-system-for-short-tandem-repeat-sequencing
#15
Deborah S B S Silva, Fernanda R Sawitzki, Melissa K R Scheible, Sarah F Bailey, Clarice S Alho, Seth A Faith
With the advent of Next-Generation Sequencing technology, sequencing of short tandem repeats (STRs) allows for a more detailed analysis when compared to size-based fragment methods (capillary electrophoresis-CE). The implementation of high-throughput sequencing can help uncover deeper genetic diversities of different populations. Subjects from the South region of Brazil present a particular and more homogeneous ancestry background when compared to other regions of the country. Both autosomal and Y- STRs have been analyzed in these individuals; however, all analyses published to date encompass data from CE-based fragment analysis...
December 16, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29259468/genetic-risk-of-klinefelter-s-syndrome-in-assisted-reproductive-technology
#16
Tamito Miki, Motoi Nagayoshi, Yoichi Takemoto, Takashi Yamaguchi, Satoru Takeda, Seiji Watanabe, Atsushi Tanaka
Aim: The main cause of Klinefelter's syndrome (KS) has been believed to be XY sperm. Accordingly, in the intracytoplasmic sperm injection treatment of patients with KS, hereditary KS has been a concern. Therefore, this study attempted to estimate the risk before and after the assisted reproductive technology. Methods: First, in order to validate the safety of the gametes of the patients with KS, a fluorescent in situ hybridization (FISH) analysis, following an original cell identification method using 1052 testicular gametes of 30 patients, was conducted...
April 2017: Reproductive Medicine and Biology
https://www.readbyqxmd.com/read/29258531/malignant-rhabdoid-tumor-in-the-renal-allograft-of-an-adult-transplant-recipient-a-unique-case-of-a-rare-tumor
#17
Jing Xiong, Tiefen Su, Pengcheng Zhu, Qilin Ao, Qiurong Ruan, Guoping Wang
BACKGROUND: Renal transplant recipients have increased risk for developing malignant diseases because of immunosuppression or donor-to-recipient transmission. Malignant rhabdoid tumor (MRT) is a rare, highly aggressive and lethal tumor primarily affecting the kidney of infants and young children. MRT has not been reported in the renal allograft of an adult recipient after kidney transplantation. CASE PRESENTATION: In this report, a 47-year-old woman who received a kidney transplantation from an infant donor and developed a mass in the transplanted kidney is presented...
December 19, 2017: Diagnostic Pathology
https://www.readbyqxmd.com/read/29247928/a-review-of-bioinformatic-methods-for-forensic-dna-analyses
#18
REVIEW
Yao-Yuan Liu, SallyAnn Harbison
Short tandem repeats, single nucleotide polymorphisms, and whole mitochondrial analyses are three classes of markers which will play an important role in the future of forensic DNA typing. The arrival of massively parallel sequencing platforms in forensic science reveals new information such as insights into the complexity and variability of the markers that were previously unseen, along with amounts of data too immense for analyses by manual means. Along with the sequencing chemistries employed, bioinformatic methods are required to process and interpret this new and extensive data...
December 12, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29245019/genetic-polymorphism-of-21-non-codis-str-loci-in-chengdu-han-population-and-its-interpopulation-analysis-between-25-populations-in-china
#19
Ye Li, Hepei Li, Guanglin He, Weibo Liang, Haibo Luo, Miao Liao, Ji Zhang, Jing Yan, Yingbi Li, Yiping Hou, Jin Wu
AGCU 21+1 STR kit contains 21 non-combined DNA index system (non-CODIS) short tandem repeats (STR) loci and a sex-determining locus amelogenin. In this study, we evaluated the genetic diversity and forensically relevant population statistics of 21 non-CODIS loci in 210 Chinese Han individuals from Chengdu city, Sichuan province, Southwest China. No significant deviations from Hardy-Weinberg equilibrium were observed within the 21 non-CODIS STR loci. The combined power of discrimination (CPD) and combined power of exclusion (CPE) were 0...
December 9, 2017: Legal Medicine
https://www.readbyqxmd.com/read/29229744/three-reportedly-unrelated-families-with-liddle-syndrome-inherited-from-a-common-ancestor
#20
Luca Pagani, Yoan Diekmann, Marco Sazzini, Sara De Fanti, Maurizio Rondinelli, Enrico Farnetti, Bruno Casali, Amelia Caretto, Francesca Novara, Orsetta Zuffardi, Paolo Garagnani, Franco Mantero, Mark G Thomas, Donata Luiselli, Ermanno Rossi
Liddle syndrome is considered a rare Mendelian hypertension. We have previously described 3 reportedly unrelated families, native of an Italian area around the Strait of Messina, carrying the same mutation (βP617L) of the epithelial sodium channel. The aims of our study were (1) to evaluate whether a close genomic relationship exists between the 3 families through the analysis of mitochondrial DNA and Y chromosome; and (2) to quantify the genomic relatedness between the patients with Liddle syndrome belonging to the 3 families and assess the hypothesis of a mutation shared through identity by descent...
December 11, 2017: Hypertension
keyword
keyword
51334
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"