keyword
MENU ▼
Read by QxMD icon Read
search

Brucella abortus

keyword
https://www.readbyqxmd.com/read/28296882/detection-and-characterization-of-brucella-spp-in-bovine-milk-in-small-scale-urban-and-peri-urban-farming-in-tajikistan
#1
Elisabeth Lindahl-Rajala, Tove Hoffman, David Fretin, Jacques Godfroid, Nosirjon Sattorov, Sofia Boqvist, Åke Lundkvist, Ulf Magnusson
Brucellosis is one of the most common zoonoses globally, and Central Asia remains a Brucella hotspot. The World Health Organization classifies brucellosis as a neglected zoonotic disease that is rarely in the spotlight for research and mainly affects poor, marginalized people. Urban and peri-urban farming is a common practice in many low-income countries, and it increases the incomes of families that are often restrained by limited economic resources. However, there is a concern that the growing number of people and livestock living close together in these areas will increase the transmission of zoonotic pathogens such as Brucella...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28284850/molecular-prevalence-of-putative-virulence-associated-genes-in-brucella-melitensis-and-brucella-abortus-isolates-from-human-and-livestock-specimens-in-iran
#2
Iman Hashemifar, Abbas Yadegar, Faramarz Masjedian Jazi, Nour Amirmozafari
Molecular prevalence of nine putative virulence factors in two more prevalent Brucella species in Iranian patients and livestock was investigated. During five years (2010-2015), 120 human and animal specimens were collected from three geographical areas of Iran. All samples were cultured in blood culture media and subcultured into Brucella agar medium. Nine primer pairs were designed for detection of VirB2, VirB5, VceC, BtpA, BtpB, PrpA, BetB, BPE275 and BSPB virulence factors using PCR and sequence analysis...
March 8, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28281771/an-evaluation-of-serological-tests-in-the-diagnosis-of-bovine-brucellosis-in-naturally-infected-cattle-in-kwazulu-natal-province-in-south-africa
#3
Songelwayo L Chisi, Yoanda Marageni, Prebashni Naidoo, Gloria Zulu, George W Akol, Henriette Van Heerden
The diagnostic sensitivity (DSe) of the Rose Bengal test (RBT), the complement fixation test (CFT), the serum agglutination test (SAT), the competitive enzyme-linked immunosorbent assay (cELISA) and the indirect ELISA (iELISA) were determined in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture (gold standard). Natural brucellosis infection status of animals was determined by culturing and identification of Brucella abortus biovar 1 from abomasal fluid, milk, hygroma fluid, lymph nodes or uterine discharges samples...
February 28, 2017: Journal of the South African Veterinary Association
https://www.readbyqxmd.com/read/28265268/brucella-and-osteoarticular-cell-activation-partners-in-crime
#4
REVIEW
Guillermo H Giambartolomei, Paula C Arriola Benitez, M Victoria Delpino
Osteoarticular brucellosis is the most common presentation of human active disease although its prevalence varies widely. The three most common forms of osteoarticular involvement are sacroiliitis, spondylitis, and peripheral arthritis. The molecular mechanisms implicated in bone damage have been recently elucidated. B. abortus induces bone damage through diverse mechanisms in which TNF-α and the receptor activator of nuclear factor kappa-B ligand (RANKL)-the natural modulator of bone homeostasis are involved...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28264909/promotion-and-rescue-of-intracellular-brucella-neotomae-replication-during-co-infection-with-legionella-pneumophila
#5
Yoon-Suk Kang, James E Kirby
We established a new Brucella neotomaein vitro model system for study of type IV secretion system-dependent (T4SS) pathogenesis in the Brucella genus. Importantly, B. neotomae is a rodent pathogen and, unlike B. abortus, melitensis, and suis, has not been observed to infect humans. It therefore can be handled more facilely using biosafety level 2 practices. More particularly, using a series of novel fluorescent protein and lux operon reporter systems to differentially label pathogens and track intracellular replication, we confirmed T4SS-dependent intracellular growth of B...
March 6, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28262966/serologic-evidence-of-brucella-infection-in-pinnipeds-along-the-coast-of-hokkaido-the-northernmost-main-island-of-japan
#6
Erika Abe, Kazue Ohishi, Tsuyoshi Ishinazaka, Ke Fujii, Tadashi Maruyama
Brucella infection in Hokkaido was serologically surveyed in four species of pinnipeds inhabiting Cape Erimo during 2008-2013 and the Shiretoko Peninsula in 1999, by enzyme-linked immunosorbent assay using Brucella abortus and B. canis as antigens. Anti-Brucella positive sera showed higher absorbance to B. abortus than B. canis in almost all samples. Anti-B. abortus antibodies were detected in serum samples from 24% (n=55) of Western Pacific harbor seals (Phoca vitulina stejnegeri) in Cape Erimo, and from 66% (n=41) of spotted seals (P...
March 6, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28261222/u-omp19-from-brucella-abortus-is-a-useful-adjuvant-for-vaccine-formulations-against-salmonella-infection-in-mice
#7
Gabriela S Risso, Marianela V Carabajal, Laura A Bruno, Andrés E Ibañez, Lorena M Coria, Karina A Pasquevich, Seung-Joo Lee, Stephen J McSorley, Gabriel Briones, Juliana Cassataro
Most pathogens infect through mucosal surfaces, and parenteral immunization typically fails to induce effective immune responses at these sites. Development of oral-administered vaccines capable of inducing mucosal as well as systemic immunity while bypassing the issues of antigen degradation and immune tolerance could be crucial for the control of enteropathogens. This study demonstrates that U-Omp19, a bacterial protease inhibitor with immunostimulatory features, coadministered with Salmonella antigens by the oral route, enhances mucosal and systemic immune responses in mice...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28248684/neonatal-brucellosis-a-case-report
#8
Abdul Rahman M Alnemri, Adnan Hadid, Shaik Asfaq Hussain, Ali M Somily, Badr H Sobaih, Abdulkarim Alrabiaah, Awad Alanazi, Zahid Shakoor, Sarah AlSubaie, Naema Meriki, Abdelmageed M Kambal
Although brucellosis is not uncommon in Saudi Arabia, neonatal brucellosis has been infrequently reported. In this case of neonatal brucellosis, Brucella abortus was isolated by blood culture from both the mother and the neonate. Serology was positive only in the mother.
February 28, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28243986/transcriptional-regulator-gntr-of-brucella-abortus-regulates-cytotoxicity-induces-the-secretion-of-inflammatory-cytokines-and-affects-expression-of-the-type-iv-secretion-system-and-quorum-sensing-system-in-macrophages
#9
Zhiqiang Li, Shuli Wang, Hui Zhang, Jinliang Zhang, Li Xi, Junbo Zhang, Chuangfu Chen
The pathogenic mechanisms of Brucella are still poorly understood. GntR is a transcriptional regulator and plays an important role in the intracellular survival of Brucella. To investigate whether GntR is involved in the cytotoxicity of Brucella abortus (B. abortus), we created a 2308ΔgntR mutant of B. abortus 2308 (S2308). Lactate dehydrogenase (LDH) cytotoxicity assays using a murine macrophage cell line (RAW 264.7) show that high-dose infection with the parental strain produces a high level of cytotoxicity to macrophages, but the 2308ΔgntR mutant exhibits a very low level of cytotoxicity, indicating that mutation of GntR impairs the cytotoxicity of B...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28183751/genome-sequences-of-human-and-livestock-isolates-of-brucella-melitensis-and-brucella-abortus-from-the-country-of-georgia
#10
Ketevan Sidamonidze, Jun Hang, Yu Yang, George Dzavashvili, Ekaterine Zhgenti, Nino Trapaidze, Paata Imnadze, Mikeljon P Nikolich
Brucellosis, which is among the most widespread global zoonotic diseases, is endemic in the nation of Georgia and causes substantial human morbidity and economic loss. Here, we report whole-genome sequences of three Brucella melitensis and seven Brucella abortus isolates from cattle, sheep, and humans that represent genetic groups discovered in Georgia.
February 9, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28167945/tlr7-and-tlr3-sense-brucella-abortus-rna-to-induce-proinflammatory-cytokine-production-but-they-are-dispensable-for-host-control-of-infection
#11
Priscila C Campos, Marco Túlio R Gomes, Erika S Guimarães, Gabriela Guimarães, Sergio C Oliveira
Brucella abortus is a Gram-negative, facultative intracellular bacterium that causes brucellosis, a worldwide zoonotic disease leading to undulant fever in humans and abortion in cattle. The immune response against this bacterium relies on the recognition of microbial pathogen-associated molecular patterns, such as lipoproteins, lipopolysaccharides, and DNA; however, the immunostimulatory potential of B. abortus RNA remains to be elucidated. Here, we show that dendritic cells (DCs) produce significant amounts of IL-12, IL-6, and IP-10/CXCL10, when stimulated with purified B...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28160160/the-unexpected-discovery-of-brucella-abortus-buck-19-vaccine-in-goats-from-ecuador-underlines-the-importance-of-biosecurity-measures
#12
Jorge Ron-Román, Dirk Berkvens, Daniela Barzallo-Rivadeneira, Alexandra Angulo-Cruz, Pablo González-Andrade, Elizabeth Minda-Aluisa, Washington Benítez-Ortíz, Jef Brandt, Richar Rodríguez-Hidalgo, Claude Saegerman
Very few, mostly old, and only preliminary serological studies of brucellosis in goats exist in Ecuador. In order to assess the current epidemiological situation, we performed a cross-sectional serological study in the goat populations of Carchi (n = 160 animals), Pichincha (n = 224 animals), and Loja provinces (n = 2024 animals). Only two positive serological results (RB negative and SAT-EDTA ≥400 IU/ml) were obtained in lactating goats from the same farm in Quito (Pichincha province). Additionally, milk was sampled from 220 animals in Pichincha province...
March 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28152976/origins-and-global-context-of-brucella-abortus-in-italy
#13
Giuliano Garofolo, Elisabetta Di Giannatale, Ilenia Platone, Katiuscia Zilli, Lorena Sacchini, Anna Abass, Massimo Ancora, Cesare Cammà, Guido Di Donato, Fabrizio De Massis, Paolo Calistri, Kevin P Drees, Jeffrey T Foster
BACKGROUND: Brucellosis is a common and chronic disease of cattle and other bovids that often causes reproductive disorders. Natural infection in cattle is caused by Brucella abortus and transmission typically occurs during abortions, calving, or nursing. Brucellosis is also a major zoonotic disease due to contamination of dairy products or contact with the tissues of infected animals. Brucellosis has been eradicated from most of the developed world in the last 40 years but persists in many regions-the disease remains prevalent in portions of Africa, the Middle East, Asia, and Central and South America, as well as in the Mediterranean basin...
February 2, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28131952/brucella-abortus-2308%C3%AE-nodv%C3%AE-nodw-double-mutant-is-highly-attenuated-and-confers-protection-against-wild-type-challenge-in-balb-c-mice
#14
Zhiqiang Li, Shuli Wang, Jinliang Zhang, Guangli Yang, Baodong Yuan, Jie Huang, Jincheng Han, Li Xi, Yanren Xiao, Chuangfu Chen, Hui Zhang
Brucellosis is an important zoonotic disease of worldwide distribution, which causes animal and human disease. However, the current Brucella abortus (B. abortus) vaccines (S19 and RB51) have several drawbacks, including residual virulence for animals and humans. Moreover, S19 cannot allow serological differentiation between infected and vaccinated animals. We constructed double deletion (ΔNodVΔNodW) mutant from virulent B. abortus 2308 (S2308) by deleting the genes encoding two-component regulatory system (TCS) in chromosome II in S2308...
January 25, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28129787/seroprevalence-and-risk-factors-associated-with-bovine-brucellosis-in-the-potohar-plateau-pakistan
#15
Shahzad Ali, Shamim Akhter, Heinrich Neubauer, Falk Melzer, Iahtasham Khan, Emmanuel Nji Abatih, Hosny El-Adawy, Muhammad Irfan, Ali Muhammad, Muhammad Waqas Akbar, Sajid Umar, Qurban Ali, Muhammad Naeem Iqbal, Abid Mahmood, Haroon Ahmed
BACKGROUND: The seroprevalence and risk factors of bovine brucellosis were studied at animal and herd level using a combination of culture, serological and molecular methods. The study was conducted in 253 randomly selected cattle herds of the Potohar plateau, Pakistan from which a total of 2709 serum (1462 cattle and 1247 buffaloes) and 2330 milk (1168 cattle and 1162 buffaloes) samples were collected. Data on risk factors associated with seroprevalence of brucellosis were collected through interviews using questionnaires...
January 28, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28126739/structure-based-mutational-studies-of-o-acetylserine-sulfhydrylase-reveal-the-reason-for-the-loss-of-cysteine-synthase-complex-formation-in-brucella-abortus
#16
Sudhaker Dharavath, Isha Raj, Samudrala Gourinath
Cysteine biosynthesis takes place via a two-step pathway in bacteria, fungi, plants and protozoan parasites, but not in humans, and hence the machinery of cysteine biosynthesis is an opportune target for therapeutics. The decameric cysteine synthase complex (CSC) is formed when the C-terminal tail of serine acetyl transferase (SAT) binds in the active site of O-acetyl serine sulfydrylase (OASS), playing a role in the regulation of this pathway. Here we show that OASS from Brucella abortus (BaOASS) does not interact with its cognate SAT C-terminal tail...
January 26, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28118557/brucellosis-transmission-between-wildlife-and-livestock-in-the-greater-yellowstone-ecosystem-inferences-from-dna-genotyping
#17
Michael P O'Brien, Albano Beja-Pereira, Neil Anderson, Ruben M Ceballos, William H Edwards, Beth Harris, Rick L Wallen, Vânia Costa
The wildlife of the Greater Yellowstone Ecosystem carries brucellosis, which was first introduced to the area by cattle in the 19th Century. Brucellosis transmission between wildlife and livestock has been difficult to study due to challenges in culturing the causative agent, Brucella abortus . We examined B. abortus transmission between American bison ( Bison bison ), Rocky Mountain elk ( Cervus elaphus nelsoni), and cattle ( Bos taurus ) using variable number tandem repeat (VNTR) markers on DNA from 98 B...
January 24, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28096613/immunotoxic-effect-of-thiamethoxam-in-immunized-mice-with-brucella-abortus-cultural-filtrate-antigen
#18
L H Salema, M J Alwan, Afaf Abdulrahman Yousif
AIM: This study was planned for determination the toxic effect of thiamethoxam (TMX) in immunized mice with Brucella abortus culture filtrate antigen (CFBAgs) (as a vaccine) and its role of TMX on decrease activity of B. abortus antigen on eliciting of humoral and cellular immunity. MATERIALS AND METHODS: To achieve these goals 60 female mice were used, 7-8 weeks age, they were divided equally into three groups (20 in each group) and treated as follows: 1(st) group: Mice were immunized with CFBAgs intraperitoneally in two doses, 2 weeks intervals with (protein concentration 2 mg\ml), 2(nd) group: Mice immunized as in the 1(st) group and was administrated orally with 1/10 lethal dose 50% of TMX (83...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28088479/three-dimensional-structure-of-full-length-ntrx-an-unusual-member-of-the-ntrc-family-of-response-regulators
#19
Ignacio Fernández, Irina Cornaciu, Mariela Del Carmen Carrica, Emiko Uchikawa, Guillaume Hoffmann, Rodrigo Sieira, José Antonio Márquez, Fernando A Goldbaum
Bacteria sense and adapt to environmental changes using two-component systems. These signaling pathways are formed by a histidine kinase that phosphorylates a response regulator (RR), which finally modulates the transcription of target genes. The bacterium Brucella abortus codes for a two-component system formed by the histidine kinase NtrY and the RR NtrX that participates in sensing low oxygen tension and generating an adaptive response. NtrX is a modular protein with REC, AAA+, and DNA-binding domains, an architecture that classifies it among the NtrC subfamily of RRs...
January 12, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28077743/seroepidemiology-of-human-brucellosis-among-blood-donors-in-southern-ethiopia-calling-attention-to-a-neglected-zoonotic-disease
#20
Bereket Workalemahu, Tsegaye Sewunet, Ayalew Astatkie
Human brucellosis is neglected in southern Ethiopia. Although traditional food processing practices and animal husbandry which increase the risk of brucellosis are common, it has not been properly studied yet. This study was conducted to determine the seroepidemiology of brucellosis among apparently healthy individuals in southern Ethiopia. In the study, blood samples were collected to screen for serum agglutinins reactive to stained antigen of Brucella abortus Standard tube titration was performed for reactive serum to determine the titer of the agglutinin...
January 11, 2017: American Journal of Tropical Medicine and Hygiene
keyword
keyword
51307
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"