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Brucella abortus

María Virginia Gentilini, Ayelén Ivana Pesce Viglietti, Paula Constanza Arriola Benitez, Andrea Elena Iglesias Molli, Gloria Edith Cerrone, Guillermo Hernán Giambartolomei, María Victoria Delpino
Brucella abortus induces an inflammatory response that stimulates the endocrine system resulting in the secretion of cortisol and dehydroepiandrosterone (DHEA). Osteoarticular brucellosis is the most common presentation of the active disease in humans, and we have previously demonstrated that B. abortus infection inhibits osteoblast function. We aimed to evaluate the role of cortisol and DHEA on osteoblast during B. abortus infection. B. abortus infection induces apoptosis and inhibits osteoblast function. DHEA treatment reversed the effect of B...
2018: Frontiers in Immunology
Jonathan Lalsiamthara, John Hwa Lee
The enzyme 4-hydroxyproline 2-epimerase (PrpA) involves in modulation of host immunity and is also reported as a potent B-cell mitogen. Live attenuated Salmonella Typhimurium (ST) vector constitutively expressing heterologous Brucella abortus PrpA protein (ST-PrpA) was inoculated in BALB/c mice in order to investigate the influence of the enzyme, on safety aspects, humoral and cellular immunity as well as protective efficacies against wild type challenges. No aggravation of morbidity was observed upon mice inoculation of ST-PrpA...
February 9, 2018: Molecular Immunology
Mary M Weber, Robert Faris
Intracellular bacteria have developed numerous strategies to hijack host vesicular trafficking pathways to form their unique replicative niches. To promote intracellular replication, the bacteria must interact with host organelles and modulate host signaling pathways to acquire nutrients and membrane for the growing parasitophorous vacuole all while suppressing activation of the immune response. To facilitate host cell subversion, bacterial pathogens use specialized secretion systems to deliver bacterial virulence factors, termed effectors, into the host cell that mimic, agonize, and/or antagonize the function of host proteins...
2018: Frontiers in Cell and Developmental Biology
Adis Softic, Kassahun Asmare, Erik Georg Granquist, Jacques Godfroid, Nihad Fejzic, Eystein Skjerve
BACKGROUND: Dairy production in Bosnia and Herzegovina exhibits limited productivity, which may partly, be explained by extensive reproductive problems of non-infectious and infectious origin. Brucella spp., Chlamydia abortus, Coxiella burnetii and Neospora caninum are common infectious causes of decreased reproductive outcomes in cattle worldwide. Little is, however, known about the disease status of herds with reduced reproductive performances. A cross-sectional study was designed to document the status of these pathogens in dairy cattle in Bosnia and Herzegovina...
February 2, 2018: BMC Veterinary Research
Pamela Altamirano-Silva, Jazmín Meza-Torres, Amanda Castillo-Zeledón, Nazareth Ruiz-Villalobos, Ana Mariel Zuñiga-Pereira, Carlos Chacón-Díaz, Edgardo Moreno, Caterina Guzmán-Verri, Esteban Chaves-Olarte
Brucella abortus is a facultative extracellular-intracellular pathogen belonging a group of α-Proteobacteria that establishes close interactions with animal cells. This bacterium enters host cells in a membrane bound compartment, avoiding the lysosomal route and reaching the endoplasmic reticulum through the action of the Type IV secretion system, VirB. In this work we demonstrate that the two-component system BvrR/BvrS senses the intracellular environment to mount the transcriptional response required for intracellular life adaptation...
January 29, 2018: Infection and Immunity
Wenlong Nan, Lide Qin, Yong Wang, Yueyong Zhang, Pengfei Tan, Yuqi Chen, Kairong Mao, Yiping Chen
BACKGROUND: Brucellosis is a widespread zoonotic disease caused by Gram-negative Brucella bacteria. Immunisation with attenuated vaccine is an effective method of prevention, but it can interfere with diagnosis. Live, attenuated Brucella abortus strain 104M has been used for the prevention of human brucellosis in China since 1965. However, at present, no fast and reliable method exists that can distinguish this strain from field strains. Single nucleotide polymorphism (SNP)-based assays offer a new approach for such discrimination...
January 24, 2018: BMC Veterinary Research
Dong-Ri Piao, Xi Liu, Dong-Dong Di, Pei Xiao, Zhong-Zhi Zhao, Li-Qing Xu, Guo-Zhong Tian, Hong-Yan Zhao, Wei-Xing Fan, Bu-Yun Cui, Hai Jiang
BACKGROUND: Brucellosis incidence in China is divided into three stages: high incidence (1950s-1960s), decline (1970s-1980s), and re-emergence (1990s-2010s). At the re-emergence stage, Brucellosis incidence grew exponentially and spread to all 32 provinces. We describe the magnitude and the etiological distribution changes in mainland China by genotyping data and emphasize its recent reemergence. We also provide the genetic diversity and molecular epidemiological characteristics of Brucella...
January 10, 2018: BMC Microbiology
Jaroslaw Sedzicki, Therese Tschon, Shyan Huey Low, Kevin Willemart, Kenneth N Goldie, Jean-Jacques Letesson, Henning Stahlberg, Christoph Dehio
Entry of the facultative intracellular pathogen Brucella into host cells results in the formation of endosomal Brucella-containing vacuoles (eBCVs) that initially traffic along the endocytic pathway. eBCV acidification triggers the expression of a type IV secretion system that translocates bacterial effector proteins into host cells. This interferes with lysosomal fusion of eBCVs and supports their maturation to replicative Brucella-containing vacuoles (rBCVs). Bacteria replicate in rBCVs to large numbers, eventually occupying most of the cytoplasmic volume...
January 12, 2018: Journal of Cell Science
Zhiqiang Li, Hui Zhang, Jinliang Zhang, Li Xi, Guangli Yang, Shuli Wang, Qingfeng Zhou, Xiaogen Zhang, Junbo Zhang
Brucellae are intracellular bacterial pathogens that cause Brucellosis, bringing great economic burdens to developing countries. The pathogenic mechanisms of Brucella are still poorly understood. Earlier immune response plays an important role in the Brucella infection. Phosphoglyceromutase (PGM) and dihydrodipicolinate reductase (DapB) were cloned, expressed, purified, and their immunocompetence was analyzed. Cytokines were detected by murine macrophages (RAW 264.7) and splenocytes that stimulated with the two recombinant proteins...
January 4, 2018: World Journal of Microbiology & Biotechnology
Huynh Tan Hop, Alisha Wehdnesday Bernardo Reyes, Tran Xuan Ngoc Huy, Lauren Togonon Arayan, WonGi Min, Hu Jang Lee, Man Hee Rhee, Hong Hee Chang, Suk Kim
Brucella abortus is a Gram-negative zoonotic pathogen for which there is no 100% effective vaccine. Phagosomes in B. abortus-infected cells fail to mature allowing the pathogen to survive and proliferate. Interleukin 10 (IL-10) promotes B. abortus persistence in macrophages by mechanisms that are not fully understood. In this study, we investigated the regulatory role of IL-10 in the immune response to B. abortus infection. B. abortus-infected macrophages were treated with either IL10 siRNA or recombinant IL-10 (rIL-10) and the expression of phagolysosome- or inflammation-related genes was evaluated by qRT-PCR and Western blot...
January 4, 2018: Journal of Biological Chemistry
Аida Daugaliyeva, Akhmetzhan Sultanov, Bekbolat Usserbayev, Sholpan Baramova, Paola Modesto, Akmaral Adambayeva, Pier Luigi Acutis, Simone Peletto
Currently, although the prevalence of brucellosis in Kazakhstan remains high, there are limited data available on the genetic diversity of circulating Brucella strains. Here, MLVA was employed to genotype a panel of 102 Brucella isolates collected from eight Kazakh regions and neighboring countries (Russia, Kyrgyzstan) during the period 1935-2017. MLVA-11 analysis classified 64 B. abortus strains into genotypes 72, 82, 331, 71, 341 and 69, while one genotype was novel, having no correspondence within the MLVA international database...
December 23, 2017: Infection, Genetics and Evolution
Juana L Vidal Arboleda, Luisa F Ortiz Roman, Martha Olivera Angel
Brucella canis is a facultative intracellular pathogen responsible for canine brucellosis, a zoonotic disease that affects canines, causing abortions and reproductive failure; and the production of non-specific symptoms in humans. In 2005 the presence of B. canis in Antioquia was demonstrated and the strains were identified as type 2. The sequencing of the genome of a field strain denoted Brucella canis str. Oliveri, showed species-specific indel events, which led us to investigate the genomic characteristics of the B...
December 22, 2017: Revista Argentina de Microbiología
Gregory Simpson, Tanguy Marcotty, Elodie Rouille, Nelson Matekwe, Jean-Jacques Letesson, Jacques Godfroid
This study shows the absence of the world's most common bacterial zoonoses caused by Brucella abortus and Brucella melitensis in cattle, goats and dogs in an agro-pastoral community in South Africa, where heifer vaccination against brucellosis with the live Strain 19 vaccine is compulsory. The study site is bordering wildlife reserves with multiple wildlife species infected with brucellosis. The results showed a low seroprevalence (1.4%) in cattle. Seroprevalence in cattle decreased with age after 4 years in females, males were less positive than females and a tissue culture from a brucellin skin test-positive male was negative...
December 23, 2017: Tropical Animal Health and Production
Jonathan Lalsiamthara, John Hwa Lee
With an objective to generate safe and effective anti-Brucella vaccine, an attenuated live Salmonella Typhimurium vector delivering heterologous Brucella immunogenic proteins SOD, Omp19, BLS, and PrpA formulated with purified Brucella abortus lipopolysaccharide was evaluated on a guinea pig model. This model represents high susceptibility to Brucella infections and showed similarities in reproducing human pathologies. On safety perspectives, the vaccine formulation induced no observable alterations on general health and histology of the vaccinated guinea pigs...
January 2018: Veterinary Immunology and Immunopathology
Xiaodong Zai, Qiaoling Yang, Ying Yin, Ruihua Li, Mengying Qian, Taoran Zhao, Yaohui Li, Jun Zhang, Ling Fu, Junjie Xu, Wei Chen
Brucella spp. are facultative intracellular pathogens that cause chronic brucellosis in humans and animals. The virulence of Brucella primarily depends on its successful survival and replication in host cells. During invasion of the host tissue, Brucella is simultaneously subjected to a variety of harsh conditions, including nutrient limitation, low pH, antimicrobial defenses, and extreme levels of reactive oxygen species (ROS) via the host immune response. This suggests that Brucella may be able to regulate its metabolic adaptation in response to the distinct stresses encountered during its intracellular infection of the host...
2017: Frontiers in Microbiology
Tobias Kerrinnes, Maria G Winter, Briana M Young, Vladimir E Diaz-Ochoa, Sebastian E Winter, Renée M Tsolis
Treatment of intracellular bacterial pathogens with antibiotic therapy often requires a long course of multiple antibiotics. A barrier to developing strategies that enhance antibiotic efficacy against these pathogens is our poor understanding of the intracellular nutritional environment that maintains bacterial persistence. The intracellular pathogen Brucella abortus survives and replicates preferentially in alternatively activated macrophages (AAM), however knowledge of the metabolic adaptations promoting exploitation of this niche is limited...
December 4, 2017: Infection and Immunity
Miriam M Costa Franco, Fernanda Marim, Erika S Guimarães, Natan R G Assis, Daiane M Cerqueira, Juliana Alves-Silva, Jerome Harms, Gary Splitter, Judith Smith, Thirumala-Devi Kanneganti, Nina M G P de Queiroz, Delia Gutman, Glen N Barber, Sergio C Oliveira
Immunity against microbes depends on recognition of pathogen-associated molecular patterns by innate receptors. Signaling pathways triggered by Brucella abortus DNA involves TLR9, AIM2, and stimulator of IFN genes (STING). In this study, we observed by microarray analysis that several type I IFN-associated genes, such as IFN-β and guanylate-binding proteins (GBPs), are downregulated in STING knockout (KO) macrophages infected with Brucella or transfected with DNA. Additionally, we determined that STING and cyclic GMP-AMP synthase (cGAS) are important to engage the type I IFN pathway, but only STING is required to induce IL-1β secretion, caspase-1 activation, and GBP2 and GBP3 expression...
December 4, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
X Cao, Z Li, Z Liu, B Fu, Y Liu, Y Shang, J Zhou, Z Jing
Animal brucellosis is a re-emerging disease in China with high prevalence in the northwest region. A total of 66 isolates of Brucella were recovered from sheep and yaks in the Inner Mongolia, Xinjiang, Qinghai and Gansu provinces of northwest China in 2015 and 2016. Using classical biotyping and the Brucella AMOS PCR assay, all isolates were identified as Brucella melitensis biovar 3 (n = 58), B. melitensis biovar 1 (n = 1), Brucella abortus (n = 5), or Brucella suis biovar 3 (n = 2), and B. melitensis biovar 3 was found to be mainly responsible for sheep brucellosis in northwest China...
November 29, 2017: Transboundary and Emerging Diseases
Luca Freddi, Maria A Damiano, Laurent Chaloin, Eugenia Pennacchietti, Sascha Al Dahouk, Stephan Köhler, Daniela De Biase, Alessandra Occhialini
Neutralophilic bacteria have developed specific mechanisms to cope with the acid stress encountered in environments such as soil, fermented foods, and host compartments. In Escherichia coli, the glutamate decarboxylase (Gad)-dependent system is extremely efficient: it requires the concerted action of glutamate decarboxylase (GadA/GadB) and of the glutamate (Glu)/γ-aminobutyrate antiporter, GadC. Notably, this system is operative also in new strains/species of Brucella, among which Brucella microti, but not in the "classical" species, with the exception of marine mammals strains...
2017: Frontiers in Microbiology
Juliana Alves-Silva, Isabela P Tavares, Erika S Guimarães, Miriam M Costa Franco, Barbara C Figueiredo, João T Marques, Gary Splitter, Sergio C Oliveira
The microtubule (MT) cytoskeleton regulates several cellular processes related to the immune system. For instance, an intricate intracellular transport mediated by MTs is responsible for the proper localization of vesicular receptors of innate immunity and its adaptor proteins. In the present study, we used nocodazole to induce MTs depolymerization and paclitaxel or recombinant (r) TIR (Toll/interleukin-1 receptor) domain containing protein (TcpB) to induce MT stabilization in bone marrow-derived macrophages infected with Brucella abortus...
2017: Frontiers in Microbiology
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