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Brucella abortus

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https://www.readbyqxmd.com/read/27913028/active-immunization-with-brucella-abortus-s19-phage-lysate-elicits-serum-igg-that-protects-guinea-pigs-against-virulent-b-%C3%A2-abortus-and-protects-mice-by-passive-immunization
#1
Lata Jain, Mayank Rawat, Saravanan Ramakrishnan, Bablu Kumar
Brucellosis is an economically important zoonosis of worldwide significance. Earlier (Jain et al., 2015) we reported methodology for generation of phage lysate preparations against Brucella abortus S19 using brucellaphage 'ϕLd'. In this study, using a fixed dose (Two mouse PD100) of lysates, the prophylactic efficacies of both plain and alum gel adjuvanted lysates were evaluated in guinea pig by direct virulent challenge and passive mouse protection test (PMPT). Strong humoral and cell mediated immune responses in guinea pigs and protection comparable to S19 vaccine was observed with low dose (1...
November 29, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27907105/epidemiology-of-brucellosis-and-genetic-diversity-of-brucella-abortus-in-kazakhstan
#2
Elena Shevtsova, Alexandr Shevtsov, Kasim Mukanov, Maxim Filipenko, Dinara Kamalova, Igor Sytnik, Marat Syzdykov, Andrey Kuznetsov, Assel Akhmetova, Mira Zharova, Talgat Karibaev, Pavel Tarlykov, Erlan Ramanculov
Brucellosis is a major zoonotic infection in Kazakhstan. However, there is limited data on its incidence in humans and animals, and the genetic diversity of prevalent strains is virtually unstudied. Additionally, there is no detailed overview of Kazakhstan brucellosis control and eradication programs. Here, we analyzed brucellosis epidemiological data, and assessed the effectiveness of eradication strategies employed over the past 70 years to counteract this infection. We also conducted multiple loci variable-number tandem repeat analysis (MLVA) of Brucella abortus strains found in Kazakhstan...
2016: PloS One
https://www.readbyqxmd.com/read/27900285/a-t4ss-effector-targets-host-cell-alpha-enolase-contributing-to-brucella-abortus-intracellular-lifestyle
#3
María I Marchesini, Susana M Morrone Seijo, Francisco F Guaimas, Diego J Comerci
Brucella abortus, the causative agent of bovine brucellosis, invades and replicates within cells inside a membrane-bound compartment known as the Brucella containing vacuole (BCV). After trafficking along the endocytic and secretory pathways, BCVs mature into endoplasmic reticulum-derived compartments permissive for bacterial replication. Brucella Type IV Secretion System (VirB) is a major virulence factor essential for the biogenesis of the replicative organelle. Upon infection, Brucella uses the VirB system to translocate effector proteins from the BCV into the host cell cytoplasm...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27899503/postreplication-roles-of-the-brucella-virb-type-iv-secretion-system-uncovered-via-conditional-expression-of-the-virb11-atpase
#4
Erin P Smith, Cheryl N Miller, Robert Child, Jennifer A Cundiff, Jean Celli
: Brucella abortus, the bacterial agent of the worldwide zoonosis brucellosis, primarily infects host phagocytes, where it undergoes an intracellular cycle within a dedicated membrane-bound vacuole, the Brucella-containing vacuole (BCV). Initially of endosomal origin (eBCV), BCVs are remodeled into replication-permissive organelles (rBCV) derived from the host endoplasmic reticulum, a process that requires modulation of host secretory functions via delivery of effector proteins by the Brucella VirB type IV secretion system (T4SS)...
November 29, 2016: MBio
https://www.readbyqxmd.com/read/27893179/ctra-controls-cell-division-and-outer-membrane-composition-of-the-pathogen-brucella-abortus
#5
Nayla Francis, Katy Poncin, Antonella Fioravanti, Victoria Vassen, Kevin Willemart, Thi Anh Phuong Ong, Luca Rappez, Jean-Jacques Letesson, Emanuele G Biondi, Xavier De Bolle
Brucella abortus is a pathogen infecting cattle, able to survive, traffic and proliferate inside host cells. It belongs to the Alphaproteobacteria, a phylogenetic group comprising bacteria with free living, symbiotic and pathogenic lifestyles. An essential regulator of cell cycle progression named CtrA was described in the model bacterium Caulobacter crescentus. This regulator is conserved in many alphaproteobacteria, but the evolution of its regulon remains elusive. Here we identified promoters that are CtrA targets using ChIP-seq and we found that CtrA binds to promoters of genes involved in cell cycle progression, in addition to numerous genes encoding outer membrane components involved in export of membrane proteins and synthesis of lipopolysaccharide...
November 28, 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27862467/combinatorial-control-of-adhesion-of-brucella-abortus-2308-to-host-cells-by-transcriptional-rewiring-of-the-trimeric-autotransporter-btae-gene
#6
Rodrigo Sieira, Magalí G Bialer, Mara S Roset, Verónica Ruiz-Ranwez, Tomás Langer, Gastón M Arocena, Estefanía Mancini, Angeles Zorreguieta
Regulatory network plasticity is a key attribute underlying changes in bacterial gene expression and a source of phenotypic diversity to interact with the surrounding environment. Here, we sought to study the transcriptional circuit of HutC, a regulator of both metabolic and virulence genes of the facultative intracellular pathogen Brucella. Using in silico and biochemical approaches, we identified a novel functional HutC-binding site upstream of btaE, a trimeric-autotransporter adhesin involved in the attachment of Brucella to host extracellular matrix components...
November 12, 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27857610/predictors-of-specific-anti-brucella-antibodies-among-humans-in-agro-pastoral-communities-in-sengerema-district-mwanza-tanzania-the-need-for-public-awareness
#7
Elifuraha B Mngumi, Mariam M Mirambo, Sospeter Wilson, Stephen E Mshana
BACKGROUND: Human brucellosis remains to be a neglected zoonotic disease among agro-pastoral communities where livestock rearing is one of the main economic activities. This study was conducted in different agro-pastoral communities in Sengerema district, Mwanza, Tanzania, to determine seroprevalence and predictors of anti-Brucella antibodies, information that may influence public awareness on the risk factors and strategies to improve the diagnosis of brucellosis in developing countries...
2016: Tropical Medicine and Health
https://www.readbyqxmd.com/read/27853717/amiata-donkey-milk-chain-animal-health-evaluation-and-milk-quality
#8
Giuseppe Ragona, Franco Corrias, Martina Benedetti, Maria Paladini, Federica Salari, Lolanda Altomonte, Mina Martini
This study presents an investigation of Amiata donkey health and quality of milk for human consumption. Thirty-one lactating dairy jennies were examined. The following samples were collected: faecal samples from the rectum of animals for parasitological examination; cervical swabs for the detection of bacteria causing reproductive disorders; and blood samples for serological diagnosis of main zoonotic (Brucella spp., Leptospira spp.) and donkey abortion agents (Brucella spp., Leptospira spp., Salmonella abortus equi, Equine viral arterithis virus, Equine herpesvirus type 1)...
June 3, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/27853716/contamination-of-bovine-sheep-and-goat-meat-with-brucella-spp
#9
Francesco Casalinuovo, Lucia Ciambrone, Antonio Cacia, Paola Rippa
A study was conducted in order to evaluate the contamination by Brucella spp. of meat from animals slaughtered because they had resulted positive for brucellosis at some time during their life. After slaughter and before delivery to market outlets, swab samples were taken from 307 carcasses of infected animals: 40 cattle, 60 sheep and 207 goats. The swabs were subsequently analysed by means of polymerase chain reaction (PCR) tests. In addition, bacteriological tests were carried out on the lymph nodes and internal organs of the same animals...
June 3, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/27847417/seroprevalence-of-brucellosis-among-cattle-slaughtered-in-three-municipal-abattoirs-of-gombe-state-northeastern-nigeria
#10
Saleh Mohammed Jajere, Naphtali Nayamanda Atsanda, Asinamai Athliamai Bitrus, Tasiu Mallam Hamisu, Ajurojo Oluwaseun Ayo
AIM: A cross-sectional study was conducted to determine the seroprevalence of bovine brucellosis among cattle slaughtered at three municipal abattoirs of Gombe State, Nigeria. MATERIALS AND METHODS: A total of 200 blood samples collected from slaughtered cattle of different breeds (Sokoto Gudali - 50, White Fulani - 102, Red bororo - 34, and Crossbreeds - 14), sex (males - 19 and females - 181), and from different locations (Billiri - 30, Yamaltu Deba - 50, and Gombe - 120) were screened for brucellosis using rose bengal plate test (RBPT), serum agglutination test (SAT), and microtiter agglutination test (MAT)...
October 2016: Veterinary World
https://www.readbyqxmd.com/read/27836044/risk-assessment-and-management-of-brucellosis-in-the-southern-greater-yellowstone-area-ii-cost-benefit-analysis-of-reducing-elk-brucellosis-prevalence
#11
Kari Boroff, Mandy Kauffman, Dannele Peck, Eric Maichak, Brandon Scurlock, Brant Schumaker
Recent cases of bovine brucellosis (Brucella abortus) in cattle (Bos taurus) and domestic bison (Bison bison) of the southern Greater Yellowstone Area (SGYA) have been traced back to free-ranging elk (Cervus elaphus). Several management activities have been implemented to reduce brucellosis seroprevalence in elk, including test-and-slaughter, low-density feeding at elk winter feedgrounds, and elk vaccination. It is unclear which of these activities are most cost-effective at reducing the risk of elk transmitting brucellosis to cattle...
November 1, 2016: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/27816477/molecular-epidemiology-of-brucella-abortus-isolated-from-cattle-in-brazil-2009-2013
#12
Mayra Silva Oliveira, Elaine Maria Seles Dorneles, Paulo Martins Filho Soares, Antônio Augusto Fonseca, Lívia Orzil, Patrícia Gomes de Souza, Andrey Pereira Lage
The aims of the present study were to genotype Brucella abortus strains isolated from cattle in Brazil between 2009 and 2013, and to analyze their distribution to support the Programa Nacional de Controle e Erradicação de Brucelose e Tuberculose (PNCEBT) (National Brucellosis and Tuberculosis Control and Eradication Program). One hundred forty B. abortus strains isolated from cattle in Brazil between 2009 and 2013 were genotyped using a set of 18 variable number of tandem repeats (VNTR) (MLVA16+HOOF-Print 3 and 4)...
November 2, 2016: Acta Tropica
https://www.readbyqxmd.com/read/27811842/inhibition-of-mhc-i-by-brucella-abortus-is-an-early-event-during-infection-and-involves-egfr-pathway
#13
Lis N Velásquez, M Ayelén Milillo, M Victoria Delpino, Aldana Trotta, M Florencia Mercogliano, Roberto G Pozner, Roxana Schillaci, Patricia V Elizalde, Guillermo H Giambartolomei, Paula Barrionuevo
Brucella abortus is able to persist inside the host despite the development of potent CD8(+) T cell responses. We have recently reported the ability of B. abortus to inhibit the IFN-γ-induced MHC-I cell surface expression on human monocytes. This phenomenon was due to the B. abortus-mediated retention of MHC-I molecules within the Golgi apparatus and was dependent on bacterial viability. However, the implications of bacterial virulence or replicative capacity and the signaling pathways remained unknown. Here, we demonstrated that the B...
November 4, 2016: Immunology and Cell Biology
https://www.readbyqxmd.com/read/27800616/nlrp12-negatively-regulates-proinflammatory-cytokine-production-and-host-defense-against-brucella-abortus
#14
Tatiana N Silveira, Marco Túlio R Gomes, Luciana S Oliveira, Priscila C Campos, Gabriela G Machado, Sergio C Oliveira
Brucella abortus is the causative agent of brucellosis, which causes abortion in domestic animals and undulant fever in humans. This bacterium infects and proliferates mainly in macrophages and dendritic cells, where it is recognized by pattern recognition receptors (PRRs) including Nod-like receptors (NLRs). Our group recently demonstrated the role of AIM2 and NLRP3 in Brucella recognition. Here, we investigated the participation of NLRP12 in innate immune response to B. abortus. We show that NLRP12 inhibits the early production of IL-12 by bone marrow-derived macrophages upon B...
November 1, 2016: European Journal of Immunology
https://www.readbyqxmd.com/read/27799972/species-specific-pcr-for-the-diagnosis-and-determination-of-antibiotic-susceptibilities-of-brucella-strains-isolated-from-tehran-iran
#15
Gholam Reza Irajian, Faramarz Masjedian Jazi, Reza Mirnejad, Vahhab Piranfar, Taghi Zahraei Salehi, Noor Amir Mozafari, Ehsanollah Ghaznavi-Rad, Mahmoud Khormali
BACKGROUND: Brucellosis is an endemic zoonotic disease in the Middle East. This study intended to design a uniplex PCR assay for the detection and differentiation of Brucella at the species level and determining the antibiotic susceptibility pattern of Brucella in Iran. METHODS: Sixty-eight Brucella specimens (38 animal and 30 human specimens) were analyzed using PCR (using one pair of primers). Antibiotic susceptibility patterns were evaluated and compared using the E-Test and disk diffusion susceptibility test...
2016: Iranian Journal of Pathology
https://www.readbyqxmd.com/read/27789633/complete-genome-sequence-of-brucella-abortus-skn-13-isolated-from-placenta-of-aborted-cattle-in-gujarat-india
#16
H C Chauhan, Bharat K Patel, B S Chandel, Kirit B Patel, A C Patel, M D Shrimali, S S Patel, A G Bhagat, Manish Rajgor, Mitul A Patel, Maulik Patel, Jitendra Kala, Bhumika Patel
Brucella abortus is generally known to cause brucellosis in cattle and buffalo. Here, we report the draft genome sequence of Brucella abortus SKN 13, isolated from aborted cattle placenta in the area of Gujarat, India, providing precious resources for comparative genomic analyses of Brucella field strains.
October 27, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27771184/brucella-abortus-s19-vaccine-protects-dairy-cattle-against-natural-infection-with-brucella-melitensis
#17
Michael van Straten, Svetlana Bardenstein, Gaby Keningswald, Menachem Banai
Brucellosis is a zoonotic disease that can cause severe illness in humans and considerable economic loss in the livestock industry. Although small ruminants are the preferential host for Brucella melitensis, this pathogen has emerged as a cause for Brucella outbreaks in cattle. S19 vaccination is implemented in many countries where B. abortus is endemic but its effectiveness against B. melitensis has not been validated. Here we show that vaccine effectiveness in preventing disease transmission between vaccinated and unvaccinated cohorts, as determined by seroconversion, was 87...
October 19, 2016: Vaccine
https://www.readbyqxmd.com/read/27771116/coxiella-burnetii-detected-in-three-species-of-endangered-north-african-gazelles-that-recently-aborted
#18
Elena García, Gerardo Espeso, Rocío Fernández, Ángel Gómez-Martín, José María Rodríguez-Linde, Christian De la Fe
Coxiella (C.) burnetii is the etiological agent of the zoonotic disease known a Q fever. This agent can infect multiple hosts although its pathogenic potential in wild ruminants has been poorly studied. The polymerase chain reaction and the serological test detected the presence of C. burnetii in a population of North African gazelles (n = 355), comprising dorcas gazelle (Gazella dorcas neglecta), dama gazelle (Nanger dama mhorr) and Cuvier's gazelle (Gazella cuvieri) which, some of them, they recently aborted...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27767061/a-dual-targeting-approach-to-inhibit-brucella-abortus-replication-in-human-cells
#19
Daniel M Czyż, Neeta Jain-Gupta, Howard A Shuman, Sean Crosson
Brucella abortus is an intracellular bacterial pathogen and an etiological agent of the zoonotic disease known as brucellosis. Brucellosis can be challenging to treat with conventional antibiotic therapies and, in some cases, may develop into a debilitating and life-threatening chronic illness. We used multiple independent assays of in vitro metabolism and intracellular replication to screen a library of 480 known bioactive compounds for novel B. abortus anti-infectives. Eighteen non-cytotoxic compounds specifically inhibited B...
October 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27765819/brucella-abortus-down-regulates-mhc-class-ii-by-the-il-6-dependent-inhibition-of-ciita-through-the-downmodulation-of-ifn-regulatory-factor-1-irf-1
#20
Lis N Velásquez, M Ayelén Milillo, M Victoria Delpino, Aldana Trotta, Pablo Fernández, Roberto G Pozner, Roland Lang, Luciana Balboa, Guillermo H Giambartolomei, Paula Barrionuevo
Brucella abortus is an intracellular pathogen capable of surviving inside of macrophages. The success of B. abortus as a chronic pathogen relies on its ability to orchestrate different strategies to evade the adaptive CD4(+) T cell responses that it elicits. Previously, we demonstrated that B. abortus inhibits the IFN-γ-induced surface expression of MHC class II (MHC-II) molecules on human monocytes, and this phenomenon correlated with a reduction in antigen presentation. However, the molecular mechanisms, whereby B...
October 20, 2016: Journal of Leukocyte Biology
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