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Brucella abortus

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https://www.readbyqxmd.com/read/28635524/serology-and-protein-electrophoresis-for-evidence-of-exposure-to-12-mink-pathogens-in-free-ranging-american-mink-neovison-vison-in-argentina
#1
Pablo Eduardo Martino, Luis Ernesto Samartino, Néstor Oscar Stanchi, Nilda Esther Radman, Eduardo Joaquín Parrado
BACKGROUND: Basic pathologic characteristics for farmed minks were previously reported worldwide. However, its status in the wild has not been studied in detail. OBJECTIVE: Serology and electrophoresis were carried out for evidence of exposure to 12 mink pathogens on two different locations. ANIMALS AND METHODS: Serology was done in 87 wild minks by reference techniques against Toxoplasma gondii, Encephalitozoon cuniculi, Neospora caninum, Brucella abortus, Mycobacterium bovis, Leptospira interrogans, canine distemper virus (CDV), canine adenovirus (CAV), canine parvovirus (CPV), rabies virus (RV), Influenza A virus (FLUAV) and Aleutian disease virus (ADV)...
December 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28629726/th2-related-immune-responses-by-the-brucella-abortus-cellular-antigens-malate-dehydrogenase-elongation-factor-and-arginase
#2
Young Bin Im, Soojin Shim, Woo Bin Park, Suk Kim, Han Sang Yoo
Brucellosis is an important zoonotic disease caused by Brucella species. The disease is difficult to control due to the intracellular survival of the bacterium and the lack of precise understanding of pathogenesis. Despite of continuous researches on the pathogenesis of Brucella spp. infection, there is still question on the pathogenesis, especially earlier immune response in the bacterial infection. Malate dehydrogenase (MDH), elongation factor (Tsf), and arginase (RocF), which showed serological reactivity, were purified after gene cloning, and their immune modulating activities were then analyzed in a murine model...
June 16, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28622869/brucella-lipopolysaccharide-reinforced-salmonella-delivering-brucella-immunogens-protects-mice-against-virulent-challenge
#3
Jonathan Lalsiamthara, John Hwa Lee
Intracellular pathogen Salmonella exhibits natural infection broadly analogous to Brucella, this phenomenon makes Salmonella a pragmatic choice for an anti-Brucella vaccine delivery platform. In this study we developed and formulated a combination of four attenuated Salmonella Typhimurium live vector strains delivering heterologous Brucella antigens (rBs), namely lumazine synthase, proline racemase subunit A, lipoprotein outer membrane protein-19, and Cu-Zn superoxide dismutase. With an aim to develop a cross-protecting vaccine, Brucella pan-species conserved rBs were selected...
June 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28609437/shifting-brucellosis-risk-in-livestock-coincides-with-spreading-seroprevalence-in-elk
#4
Angela Brennan, Paul C Cross, Katie Portacci, Brandon M Scurlock, William H Edwards
Tracking and preventing the spillover of disease from wildlife to livestock can be difficult when rare outbreaks occur across large landscapes. In these cases, broad scale ecological studies could help identify risk factors and patterns of risk to inform management and reduce incidence of disease. Between 2002 and 2014, 21 livestock herds in the Greater Yellowstone Area (GYA) were affected by brucellosis, a bacterial disease caused by Brucella abortus, while no affected herds were detected between 1990 and 2001...
2017: PloS One
https://www.readbyqxmd.com/read/28604602/highly-sensitive-bacteriophage-based-detection-of-brucella-abortus-in-mixed-culture-and-spiked-blood
#5
Kirill V Sergueev, Andrey A Filippov, Mikeljon P Nikolich
For decades, bacteriophages (phages) have been used for Brucella species identification in the diagnosis and epidemiology of brucellosis. Traditional Brucella phage typing is a multi-day procedure including the isolation of a pure culture, a step that can take up to three weeks. In this study, we focused on the use of brucellaphages for sensitive detection of the pathogen in clinical and other complex samples, and developed an indirect method of Brucella detection using real-time quantitative PCR monitoring of brucellaphage DNA amplification via replication on live Brucella cells...
June 10, 2017: Viruses
https://www.readbyqxmd.com/read/28588127/a-6-nucleotide-regulatory-motif-within-the-abcr-small-rnas-of-brucella-abortus-mediates-host-pathogen-interactions
#6
Lauren M Sheehan, Clayton C Caswell
In Brucella abortus, two small RNAs (sRNAs), AbcR1 and AbcR2, are responsible for regulating transcripts encoding ABC-type transport systems. AbcR1 and AbcR2 are required for Brucella virulence, as a double chromosomal deletion of both sRNAs results in attenuation in mice. Although these sRNAs are responsible for targeting transcripts for degradation, the mechanism utilized by the AbcR sRNAs to regulate mRNA in Brucella has not been described. Here, two motifs (M1 and M2) were identified in AbcR1 and AbcR2, and complementary motif sequences were defined in AbcR-regulated transcripts...
June 6, 2017: MBio
https://www.readbyqxmd.com/read/28559292/brucella-abortus-induces-a-warburg-shift-in-host-metabolism-that-is-linked-to-enhanced-intracellular-survival-of-the-pathogen
#7
Daniel M Czyż, Jonathan Willett, Sean Crosson
Intracellular bacterial pathogens exploit host cell resources to replicate and survive inside the host. Targeting these host systems is one promising approach to developing novel antimicrobials to treat intracellular infections. We show that human macrophage-like cells infected with Brucella abortus undergo a metabolic shift characterized by attenuated tricarboxylic acid cycle metabolism, reduced amino acid consumption, altered mitochondrial localization, and increased lactate production. This shift to an aerobic glycolytic state resembles the Warburg effect, a change in energy production that is well-described in cancer cells, and also occurs in activated inflammatory cells...
May 30, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28535754/a-comprehensive-proteogenomic-study-of-the-human-brucella-vaccine-strain-104%C3%A2-m
#8
Xiaodong Zai, Qiaoling Yang, Kun Liu, Ruihua Li, Mengying Qian, Taoran Zhao, Yaohui Li, Ying Yin, Dayong Dong, Ling Fu, Shanhu Li, Junjie Xu, Wei Chen
BACKGROUND: Brucella spp. are Gram-negative, facultative intracellular pathogens that cause brucellosis in both humans and animals. The B. abortus vaccine strain 104 M is the only vaccine available in China for the prevention of brucellosis in humans. Although the B. abortus 104 M genome has been fully sequenced, the current genome annotations are not yet complete. In addition, the main mechanisms underpinning its residual toxicity and vaccine-induced immune protection have yet to be elucidated...
May 23, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28533142/evaluation-of-plasma-sphingosine-1-phosphate-hepcidin-and-cardiovascular-damage-biomarkers-cardiac-troponin-i-and-homocysteine-in-rats-infected-with-brucellosis-and-vaccinated-rev-1-rb-51
#9
Kaveh Azimzadeh, Reza Nasrollahi Nargesabad, Nasim Vousooghi
Brucellosis is known as one of important zoonosis. Studying the histological and biochemical effects of the disease could help to increase our knowledge about it. The aim of the present study was to evaluate changes of plasma parameters after intraperitoneal injection of two species of Brucella (Brucella melitensis and Brucella abortus) and two vaccines (Rev-1, RB-51) in the rat. Forty male rats were divided into five groups (n = 8 in each group). Two groups received suspensions of Brucella abortus and Brucella melitensis and two other groups were injected intraperitoneally with two mentioned vaccines and the last group received only distilled water...
May 19, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28529959/getting-inside-type-i-ifns-type-i-ifns-in-intracellular-bacterial-infections
#10
REVIEW
Deann T Snyder, Jodi F Hedges, Mark A Jutila
Type I interferons represent a unique and complex group of cytokines, serving many purposes during innate and adaptive immunity. Discovered in the context of viral infections, type I IFNs are now known to have myriad effects in infectious and autoimmune disease settings. Type I IFN signaling during bacterial infections is dependent on many factors including whether the infecting bacterium is intracellular or extracellular, as different signaling pathways are activated. As such, the repercussions of type I IFN induction can positively or negatively impact the disease outcome...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28508154/estimating-loss-of-brucella-abortus-antibodies-from-age-specific-serological-data-in-elk
#11
J A Benavides, D Caillaud, B M Scurlock, E J Maichak, W H Edwards, P C Cross
Serological data are one of the primary sources of information for disease monitoring in wildlife. However, the duration of the seropositive status of exposed individuals is almost always unknown for many free-ranging host species. Directly estimating rates of antibody loss typically requires difficult longitudinal sampling of individuals following seroconversion. Instead, we propose a Bayesian statistical approach linking age and serological data to a mechanistic epidemiological model to infer brucellosis infection, the probability of antibody loss, and recovery rates of elk (Cervus canadensis) in the Greater Yellowstone Ecosystem...
May 15, 2017: EcoHealth
https://www.readbyqxmd.com/read/28506887/molecular-investigation-of-virulence-factors-of-brucella-melitensis-and-brucella-abortus-strains-isolated-from-clinical-and-non-clinical-samples
#12
Reza Mirnejad, Faramarz Masjedian Jazi, Shayan Mostafaei, Mansour Sedighi
Brucella is zoonotic pathogen that induces abortion and sterility in domestic mammals and chronic infections in humans called Malta fever. It is a facultative intracellular potential pathogen with high infectivity. The virulence of Brucella is dependent upon its potential virulence factors such as enzymes and cell envelope associated virulence genes. The aim of this study was to investigate the Brucella virulence factors among strains isolated from humans and animals in different parts of Iran. Seventy eight strains of Brucella species isolated from suspected human and animal cases from several provinces of Iran during 2015-2016 and identified by phenotypic and molecular methods...
May 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28506316/characterization-of-novel-omp31-antigenic-epitopes-of-brucella-melitensis-by-monoclonal-antibodies
#13
Jinfeng Li, Feihuan Hu, Shouyi Chen, Peifang Luo, Zuoping He, Wenjing Wang, Jean-Pierre Allain, Chengyao Li
BACKGROUND: Brucellosis is a severe zoonotic disease worldwide. Detection and identification of Brucella species are essential to prevent or treat brucellosis in humans and animals. The outer membrane protein-31 (Omp31) is a major protein of Brucellae except for B. abortus, while the Omp31 antigenic epitopes have not been extensively characterized yet. RESULTS: A total of 22 monoclonal antibodies (mAbs) were produced against Omp31 of Brucella (B.) melitensis, of which 13 recognized five linear epitopes, 7 reacted with semi-conformational epitopes and 2 reacted with conformational epitopes, respectively...
May 15, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28466240/incidence-and-knowledge-of-bovine-brucellosis-in-kahuro-district-murang-a-county-kenya
#14
Janeffer N Njuguna, Michael M Gicheru, Lucy M Kamau, Peter M Mbatha
Brucellosis in cattle is a zoonosis mainly caused by Brucella abortus. In Kenya, the disease is widespread, but its prevalence is largely unknown. The objective of this study was to investigate incidence rates of brucellosis and farmers' knowledge on the disease in Kahuro district, Murang'a County. In this study, 150 pooled milk samples were collected from 75 milk collection centers and tested. Subsequently, 230 milk samples were collected from farmers in 16 collection centers in Wangu and Mugoiri divisions whose pooled samples gave positive results...
May 3, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28460642/multi-locus-variable-number-tandem-repeat-analysis-of-chinese-brucella-strains-isolated-from-1953-to-2013
#15
Guo-Zhong Tian, Bu-Yun Cui, Dong-Ri Piao, Hong-Yan Zhao, Lan-Yu Li, Xi Liu, Pei Xiao, Zhong-Zhi Zhao, Li-Qing Xu, Hai Jiang, Zhen-Jun Li
BACKGROUND: Brucellosis was a common human and livestock disease caused by Brucella strains, the category B priority pathogens by the US Center for Disease Control (CDC). Identified as a priority disease in human and livestock populations, the increasing incidence in recent years in China needs urgent control measures for this disease but the molecular background important for monitoring the epidemiology of Brucella strains at the national level is still lacking. METHODS: A total of 600 Brucella isolates collected during 60 years (from 1953 to 2013) in China were genotyped by multiple locus variable-number tandem repeat analysis (MLVA) and the variation degree of MLVA11 loci was calculated by the Hunter Gaston Diversity Index (HGDI) values...
May 2, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28452907/in-a-mouse-model-of-sepsis-hepcidin-ablation-ameliorates-anemia-more-effectively-than-iron-and-erythropoietin-treatment
#16
Eeman Khorramian, Eileen Fung, Kristine Chua, Victoria Gabayan, Tomas Ganz, Elizabeta Nemeth, Airie Kim
Intensive Care Unit (ICU) anemia is an extreme version of anemia of inflammation (AI) that occurs commonly in critically ill patients and is associated with increased morbidity and mortality. Currently available therapies for ICU anemia have shown inconsistent efficacies in clinical trials. We conducted a systematic study of the effects of early versus delayed iron (Fe) and/or erythropoietin (EPO) therapy in our previously characterized mouse model of ICU anemia based on an injection of heat-killed Brucella abortus (BA)...
April 27, 2017: Shock
https://www.readbyqxmd.com/read/28443226/a-novel-pcr-assay-for-detecting-brucella-abortus-and-brucella-melitensis
#17
Saeed Alamian, Majid Esmaelizad, Taghi Zahraei, Afshar Etemadi, Mohsen Mohammadi, Davoud Afshar, Soheila Ghaderi
OBJECTIVES: Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis. METHODS: All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all available Brucella sequences. A unique repeat sequence (URS) locus on chromosome 1 could differentiate Brucella abortus from Brucella melitensis...
February 2017: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/28398652/brucella-abortus-activated-microglia-induce-neuronal-death-through-primary-phagocytosis
#18
Ana M Rodríguez, M Victoria Delpino, M Cruz Miraglia, Miriam M Costa Franco, Paula Barrionuevo, Vida A Dennis, Sergio C Oliveira, Guillermo H Giambartolomei
Inflammation has long been implicated as a contributor to pathogenesis in neurobrucellosis. Many of the associated neurocognitive symptoms of neurobrucellosis may be the result of neuronal dysfunction resulting from the inflammatory response induced by Brucella abortus infection in the central nervous system. In this manuscript, we describe an immune mechanism for inflammatory activation of microglia that leads to neuronal death upon B. abortus infection. B. abortus was unable to infect or harm primary cultures of mouse neurons...
April 11, 2017: Glia
https://www.readbyqxmd.com/read/28391992/protein-protein-dna-dna-and-dna-protein-based-vaccination-strategies-using-truncated-omp2b-against-brucella-infection-in-balb-c-mice
#19
Maryam Golshani, Sima Rafati, Mehdi Nejati-Moheimani, Somaye Pourabdi, Amin Arsang, Saeid Bouzari
The purpose of the present study was to evaluate the immunogenicity and protective efficacy of the truncated form of outer membrane protein 2b (TOmp2b) from Brucella abortus in BALB/c mice. Three immunization regimens Protein/Protein, DNA/DNA and DNA/Protein were used. Immunization of mice with all vaccine strategies elicited a strong specific IgG responses (IgG2a titers over IgG1) and provided T helper1 (Th1) oriented immune responses. Furthermore, Protein/Protein (Pro/Pro-) and DNA/Pro- vaccinated groups conferred protection levels against B...
June 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28376905/characterization-of-brucella-abortus-mutant-strain-%C3%AE-22915-a-potential-vaccine-candidate
#20
Yanqing Bao, Mingxing Tian, Peng Li, Jiameng Liu, Chan Ding, Shengqing Yu
Brucellosis, caused by Brucella spp., is an important zoonosis worldwide. Vaccination is an effective strategy for protection against Brucella infection in livestock in developing countries and in wildlife in developed countries. However, current vaccine strains including S19 and RB51 are pathogenic to humans and pregnant animals, limiting their use. In this study, we constructed the Brucella abortus (B. abortus) S2308 mutant strain Δ22915, in which the putative lytic transglycosylase gene BAB_RS22915 was deleted...
April 4, 2017: Veterinary Research
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