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Brucella abortus

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https://www.readbyqxmd.com/read/28096613/immunotoxic-effect-of-thiamethoxam-in-immunized-mice-with-brucella-abortus-cultural-filtrate-antigen
#1
L H Salema, M J Alwan, Afaf Abdulrahman Yousif
AIM: This study was planned for determination the toxic effect of thiamethoxam (TMX) in immunized mice with Brucella abortus culture filtrate antigen (CFBAgs) (as a vaccine) and its role of TMX on decrease activity of B. abortus antigen on eliciting of humoral and cellular immunity. MATERIALS AND METHODS: To achieve these goals 60 female mice were used, 7-8 weeks age, they were divided equally into three groups (20 in each group) and treated as follows: 1(st) group: Mice were immunized with CFBAgs intraperitoneally in two doses, 2 weeks intervals with (protein concentration 2 mg\ml), 2(nd) group: Mice immunized as in the 1(st) group and was administrated orally with 1/10 lethal dose 50% of TMX (83...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28088479/three-dimensional-structure-of-full-length-ntrx-an-unusual-member-of-the-ntrc-family-of-response-regulators
#2
Ignacio Fernández, Irina Cornaciu, Mariela Del Carmen Carrica, Emiko Uchikawa, Guillaume Hoffmann, Rodrigo Sieira, José Antonio Márquez, Fernando A Goldbaum
Bacteria sense and adapt to environmental changes using two-component systems (TCS). These signaling pathways are formed by a histidine kinase (HK) that phosphorylates a response regulator (RR), which finally modulates the transcription of target genes. The bacterium Brucella abortus codes for a TCS formed by the HK NtrY and the RR NtrX that participates in sensing low oxygen tension and in generating an adaptive response. NtrX is a modular protein with REC, AAA+ and DNA binding domains, an architecture that classifies it among the NtrC subfamily of RRs...
January 11, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28077743/seroepidemiology-of-human-brucellosis-among-blood-donors-in-southern-ethiopia-calling-attention-to-a-neglected-zoonotic-disease
#3
Bereket Workalemahu, Tsegaye Sewunet, Ayalew Astatkie
Human brucellosis is neglected in southern Ethiopia. Although traditional food processing practices and animal husbandry which increase the risk of brucellosis are common, it has not been properly studied yet. This study was conducted to determine the seroepidemiology of brucellosis among apparently healthy individuals in southern Ethiopia. In the study, blood samples were collected to screen for serum agglutinins reactive to stained antigen of Brucella abortus Standard tube titration was performed for reactive serum to determine the titer of the agglutinin...
January 11, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28068003/brucella-abortus-is-prevalent-in-both-humans-and-animals-in-bangladesh
#4
A K M A Rahman, C Saegerman, D Berkvens, F Melzer, H Neubauer, D Fretin, E Abatih, N Dhand, M P Ward
To determine the role of different Brucella (B.) spp. in Bangladesh, 62 animal samples and 500 human sera were tested. Animal samples from cattle, goats and sheep (including milk, bull semen, vaginal swabs and placentas) were cultured for Brucella spp. Three test-positive human sera and all animal samples were screened by Brucella genus-specific real-time PCR (RT-PCR), and positive samples were then tested by IS711 RT-PCR to detect B. abortus and B. melitensis DNA. Only B. abortus DNA was amplified from 13 human and six animal samples...
January 9, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28018318/cox-2-inhibition-reduces-brucella-bacterial-burden-in-draining-lymph-nodes
#5
Aurélie Gagnaire, Laurent Gorvel, Alexia Papadopoulos, Kristine Von Bargen, Jean-Louis Mège, Jean-Pierre Gorvel
Brucella is a Gram-negative facultative intracellular bacterium responsible for a chronic disease known as brucellosis, the most widespread re-emerging zoonosis worldwide. Establishment of a Th1-mediated immune response characterized by the production of IL-12 and IFNγ is essential to control the disease. Leukotrienes derived from arachidonic acid (AA) metabolism are known to negatively regulate a protective Th1 immune response against bacterial infections. Here, using genomics approaches we demonstrate that Brucella abortus strongly stimulates the prostaglandin (PG) pathway in dendritic cells (DC)...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28017899/nocodazole-treatment-interrupted-brucella-abortus-invasion-in-raw-264-7-cells-and-successfully-attenuated-splenic-proliferation-with-enhanced-inflammatory-response-in-mice
#6
Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Lauren Togonon Arayan, Tran Xuan Ngoc Huy, Wongi Min, Hu Jang Lee, Hong Hee Chang, Suk Kim
Brucellosis is one of the most important and widespread zoonosis worldwide responsible for serious economic losses and considerable public health burden. In this study, we investigated the modulatory effect of a microtubule-inhibitor, nocodazole, on B. abortus infection in murine macrophages and in a mouse model. Nocodazole activated macrophages and directly inhibited the growth of Brucella in a dose-dependent manner. Nocodazole increased adhesion but reduced invasion and intracellular growth of Brucella in macrophages although it did not affect co-localization of Brucella with LAMP-1...
December 23, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28012988/the-host-immune-enhancing-agent-korean-red-ginseng-oil-successfully-attenuates-brucella-abortus-infection-in-a-murine-model
#7
Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Lauren Togonon Arayan, Tran Xuan Ngoc Huy, Soo Jong Park, Kwang Dong Kim, WonGi Min, Hu Jang Lee, Man Hee Rhee, Yi-Seong Kwak, Suk Kim
ETHNOPHARMACOLOGICAL RELEVANCE: Panax ginseng Meyer (Araliaceae), is one of the most valuable traditional Chinese medicines and is used for the treatment of various human diseases. In this study, we elucidated the protective mechanism of the essential oil from Korean red ginseng (RGO) against Brucella infection. MATERIALS AND METHODS: The effects of RGO on Brucella abortus viability, NO production, uptake and intracellular growth in macrophages were investigated...
December 21, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27994212/intracellular-trafficking-modulation-by-ginsenoside-rg3-inhibits-brucella-abortus-uptake-and-intracellular-survival-within-raw-264-7-cells
#8
Tran Xuan Ngoc Huy, Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Lauren Togonon Arayan, WonGi Min, Hu Jang Lee, Man Hee Rhee, Hong Hee Chang, Suk Kim
Ginsenoside Rg3, a saponin extracted from Ginseng, has various pharmacological and biological activities, however its effects against Brucella infection are still unclear. Herein, the inhibitory effects of ginsenoside Rg3 against intracellular parasitic Brucella infection were evaluated through bacterial infection, adherence assays, and LAMP-1 colocalization, as well as immunoblotting and FACS for detecting MAPKs signaling proteins and F-actin polymerization, respectively. The internalization, intracellular growth and adherence of Brucella (B...
December 20, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27994018/a-conserved-abc-transport-system-regulated-by-the-general-stress-response-pathways-of-alpha-and-gammaproteobacteria
#9
Julien Herrou, Jonathan W Willett, Daniel M Czyż, Gyorgy Babnigg, Youngchang Kim, Sean Crosson
: Brucella abortus σ(E1) is an EcfG-family sigma factor that regulates transcription of dozens of genes in response to diverse stress conditions, and is required for maintenance of chronic infection in a mouse model. A putative ATP-binding cassette transporter operon, bab1_0223-bab1_0226, is among the most highly activated gene sets in the σ(E1) regulon. The proteins encoded by this operon resemble quaternary ammonium compatible solute importers, but are most similar in sequence to the broadly conserved YehZYXW system, which remains largely uncharacterized...
December 19, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27955740/control-of-animal-brucellosis-the-malaysian-experience
#10
REVIEW
M Zamri-Saad, M I Kamarudin
Brucellosis is a zoonotic disease characterized by reproductive failure in animals and undulent fever in humans. In cattle, it is caused by Brucella abortus while in goats by Brucella melitensis, the main cause of brucellosis in humans. Brucellosis in livestock has been associated with importation of animals from breeder herd of unknown disease status. The prevalence of bovine brucellosis Brucella abortus in 2014 ranged between 1% and 2% in Thailand and Indonesia, and 4%-5% in Malaysia and Myanmar. Prevalence of goat brucellosis Brucella melitensis is approximately 1% in Malaysia and Thailand...
December 2016: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27938682/comparison-of-potential-protection-conferred-by-three-immunization-strategies-protein-protein-dna-dna-and-dna-protein-against-brucella-infection-using-omp2b-in-balb-c-mice
#11
Maryam Golshani, Sima Rafati, Mehdi Nejati-Moheimani, Melina Ghasemian, Saeid Bouzari
In the present study, immunogenicity and protective efficacy of the Brucella outer membrane protein 2b (Omp2b) was evaluated in BALB/c mice using Protein/Protein, DNA/DNA and DNA/Protein vaccine strategies. Immunization of mice with three vaccine regimens elicited a strong specific IgG response (higher IgG2a titers over IgG1 titers) and provided Th1-oriented immune response. Vaccination of BALB/c mice with the DNA/Pro regimen induced higher levels of IFN-γ/IL-2 and conferred more protection levels against B...
December 25, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27938677/first-evaluation-of-an-influenza-viral-vector-based-brucella-abortus-vaccine-in-sheep-and-goats-assessment-of-safety-immunogenicity-and-protective-efficacy-against-brucella-melitensis-infection
#12
Kaissar Tabynov, Bolat Yespembetov, Nurali Matikhan, Sholpan Ryskeldinova, Nadezhda Zinina, Zhailaubay Kydyrbayev, Nurika Assanzhanova, Kairat Tabynov, Gourapura J Renukaradhya, Gulnara Mukhitdinova, Abylai Sansyzbay
Previously we developed and evaluated a candidate influenza viral vector based Brucella abortus vaccine (Flu-BA) administered with a potent adjuvant Montanide Gel01 in cattle, which was found safe and highly effective. This study was aimed to establish a proof-of-concept of the efficacy of Flu-BA vaccine formulation in sheep and goats. We vaccinated sheep and goats with Flu-BA vaccine and as a positive control vaccinated a group of animals with a commercial B. melitensis Rev.1 vaccine. Clinically, both Flu-BA and Rev...
December 25, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27934819/trend-of-human-brucellosis-over-a-decade-at-tertiary-care-centre-in-north-karnataka
#13
D P Patil, G S Ajantha, C Shubhada, P A Jain, A Kalabhavi, P C Shetty, M Hosamani, S Appannanavar, R D Kulkarni
BACKGROUND: Brucellosis is an important zoonotic disease. India having a major agrarian population is expected to have a higher prevalence. However, due to lack of laboratory facility or awareness among clinicians, the disease is largely underreported. The aim of this study was to know the prevalence and trend of human brucellosis over a decade, in patients attending a teaching hospital in North Karnataka, and to understand their geographical distribution. MATERIALS AND METHODS: The study was conducted from January 2006 to December 2015 at a tertiary care teaching hospital in North Karnataka...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27913028/active-immunization-with-brucella-abortus-s19-phage-lysate-elicits-serum-igg-that-protects-guinea-pigs-against-virulent-b-%C3%A2-abortus-and-protects-mice-by-passive-immunization
#14
Lata Jain, Mayank Rawat, Saravanan Ramakrishnan, Bablu Kumar
Brucellosis is an economically important zoonosis of worldwide significance. Earlier (Jain et al., 2015) we reported methodology for generation of phage lysate preparations against Brucella abortus S19 using brucellaphage 'ϕLd'. In this study, using a fixed dose (Two mouse PD100) of lysates, the prophylactic efficacies of both plain and alum gel adjuvanted lysates were evaluated in guinea pig by direct virulent challenge and passive mouse protection test (PMPT). Strong humoral and cell mediated immune responses in guinea pigs and protection comparable to S19 vaccine was observed with low dose (1...
November 29, 2016: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/27907105/epidemiology-of-brucellosis-and-genetic-diversity-of-brucella-abortus-in-kazakhstan
#15
Elena Shevtsova, Alexandr Shevtsov, Kasim Mukanov, Maxim Filipenko, Dinara Kamalova, Igor Sytnik, Marat Syzdykov, Andrey Kuznetsov, Assel Akhmetova, Mira Zharova, Talgat Karibaev, Pavel Tarlykov, Erlan Ramanculov
Brucellosis is a major zoonotic infection in Kazakhstan. However, there is limited data on its incidence in humans and animals, and the genetic diversity of prevalent strains is virtually unstudied. Additionally, there is no detailed overview of Kazakhstan brucellosis control and eradication programs. Here, we analyzed brucellosis epidemiological data, and assessed the effectiveness of eradication strategies employed over the past 70 years to counteract this infection. We also conducted multiple loci variable-number tandem repeat analysis (MLVA) of Brucella abortus strains found in Kazakhstan...
2016: PloS One
https://www.readbyqxmd.com/read/27900285/a-t4ss-effector-targets-host-cell-alpha-enolase-contributing-to-brucella-abortus-intracellular-lifestyle
#16
María I Marchesini, Susana M Morrone Seijo, Francisco F Guaimas, Diego J Comerci
Brucella abortus, the causative agent of bovine brucellosis, invades and replicates within cells inside a membrane-bound compartment known as the Brucella containing vacuole (BCV). After trafficking along the endocytic and secretory pathways, BCVs mature into endoplasmic reticulum-derived compartments permissive for bacterial replication. Brucella Type IV Secretion System (VirB) is a major virulence factor essential for the biogenesis of the replicative organelle. Upon infection, Brucella uses the VirB system to translocate effector proteins from the BCV into the host cell cytoplasm...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27899503/postreplication-roles-of-the-brucella-virb-type-iv-secretion-system-uncovered-via-conditional-expression-of-the-virb11-atpase
#17
Erin P Smith, Cheryl N Miller, Robert Child, Jennifer A Cundiff, Jean Celli
: Brucella abortus, the bacterial agent of the worldwide zoonosis brucellosis, primarily infects host phagocytes, where it undergoes an intracellular cycle within a dedicated membrane-bound vacuole, the Brucella-containing vacuole (BCV). Initially of endosomal origin (eBCV), BCVs are remodeled into replication-permissive organelles (rBCV) derived from the host endoplasmic reticulum, a process that requires modulation of host secretory functions via delivery of effector proteins by the Brucella VirB type IV secretion system (T4SS)...
November 29, 2016: MBio
https://www.readbyqxmd.com/read/27893179/ctra-controls-cell-division-and-outer-membrane-composition-of-the-pathogen-brucella-abortus
#18
Nayla Francis, Katy Poncin, Antonella Fioravanti, Victoria Vassen, Kevin Willemart, Thi Anh Phuong Ong, Luca Rappez, Jean-Jacques Letesson, Emanuele G Biondi, Xavier De Bolle
Brucella abortus is a pathogen infecting cattle, able to survive, traffic and proliferate inside host cells. It belongs to the Alphaproteobacteria, a phylogenetic group comprising bacteria with free living, symbiotic and pathogenic lifestyles. An essential regulator of cell cycle progression named CtrA was described in the model bacterium Caulobacter crescentus. This regulator is conserved in many alphaproteobacteria, but the evolution of its regulon remains elusive. Here we identified promoters that are CtrA targets using ChIP-seq and we found that CtrA binds to promoters of genes involved in cell cycle progression, in addition to numerous genes encoding outer membrane components involved in export of membrane proteins and synthesis of lipopolysaccharide...
November 28, 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27862467/combinatorial-control-of-adhesion-of-brucella-abortus-2308-to-host-cells-by-transcriptional-rewiring-of-the-trimeric-autotransporter-btae-gene
#19
Rodrigo Sieira, Magalí G Bialer, Mara S Roset, Verónica Ruiz-Ranwez, Tomás Langer, Gastón M Arocena, Estefanía Mancini, Angeles Zorreguieta
Regulatory network plasticity is a key attribute underlying changes in bacterial gene expression and a source of phenotypic diversity to interact with the surrounding environment. Here, we sought to study the transcriptional circuit of HutC, a regulator of both metabolic and virulence genes of the facultative intracellular pathogen Brucella. Using in silico and biochemical approaches, we identified a novel functional HutC-binding site upstream of btaE, a trimeric-autotransporter adhesin involved in the attachment of Brucella to host extracellular matrix components...
November 12, 2016: Molecular Microbiology
https://www.readbyqxmd.com/read/27857610/predictors-of-specific-anti-brucella-antibodies-among-humans-in-agro-pastoral-communities-in-sengerema-district-mwanza-tanzania-the-need-for-public-awareness
#20
Elifuraha B Mngumi, Mariam M Mirambo, Sospeter Wilson, Stephen E Mshana
BACKGROUND: Human brucellosis remains to be a neglected zoonotic disease among agro-pastoral communities where livestock rearing is one of the main economic activities. This study was conducted in different agro-pastoral communities in Sengerema district, Mwanza, Tanzania, to determine seroprevalence and predictors of anti-Brucella antibodies, information that may influence public awareness on the risk factors and strategies to improve the diagnosis of brucellosis in developing countries...
2016: Tropical Medicine and Health
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