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Brucella abortus

Alisha Wehdnesday Bernardo Reyes, Lauren Togonon Arayan, Huynh Tan Hop, Tran Xuan Ngoc Huy, Vu Hai Son, WonGi Min, Hu Jang Lee, Suk Kim
In this study, we investigated the effects of gallic acid (GA) in intracellular signaling within murine macrophages and its contribution to host immunity during Brucella infection. In vitro analysis revealed that GA treatment decreased F-actin content and suppression of p38α phosphorylation level. In vivo analysis showed that GA treatment reduced inflammation and proliferation of Brucella in spleens of mice in comparison to PBS treatment yielding a significant protection unit. For the analysis of immune response, the uninfected GA-treated mice showed increased production of IFN-γ and MCP-1, and the Brucella-infected GA-treated mice showed elevated levels of IL-12p70, TNF, IFN-γ, MCP-1, IL-10 and IL-6 in comparison to negative and positive control groups, respectively...
April 19, 2018: Microbial Pathogenesis
Huynh Tan Hop, Lauren Togonon Arayan, Tran Xuan Ngoc Huy, Alisha Wehdnesday Bernardo Reyes, WonGi Min, Hu Jang Lee, Soo Jong Park, Hong Hee Chang, Suk Kim
In this study, we assessed the protective efficacy of single subunit vaccines, encoded by the B. abortus 544 genes aspC, dps, yaeC and inpB, against B. abortus infection in mice. First, immunization with these antigens, with the exception of the YaeC protein, was found to elicit both humoral and cellular immune responses with IgG2a being dominant over IgG1. In addition, a massive production of IFN-γ but lower degree of IL-10 was observed, suggesting that all three antigens were able to induce predominantly cell-mediated immunity in response to B...
April 17, 2018: Vaccine
Amaia Zúñiga-Ripa, Thibault Barbier, Leticia Lázaro-Antón, María J de Miguel, Raquel Conde-Álvarez, Pilar M Muñoz, Jean J Letesson, Maite Iriarte, Ignacio Moriyón
Bacteria of the genus Brucella infect a range of vertebrates causing a worldwide extended zoonosis. The best-characterized brucellae infect domestic livestock, behaving as stealthy facultative intracellular parasites. This stealthiness depends on envelope molecules with reduced pathogen-associated molecular patterns, as revealed by the low lethality and ability to persist in mice of these bacteria. Infected cells are often engorged with brucellae without signs of distress, suggesting that stealthiness could also reflect an adaptation of the parasite metabolism to use local nutrients without harming the cell...
2018: Frontiers in Microbiology
Rubina Paradiso, Massimiliano Orsini, Daniela Criscuolo, Rosanna Borrelli, Ornella Valvini, Cesare Cammà, Maria Luisa Chiusano, Giorgio Galiero, Giorgia Borriello
Brucellosis is a zoonotic disease that affects both humans and animals. Its distribution is global, concentrated in the Mediterranean area, India, Central Asia, and Latin America. Here, we present a complete genome assembly of 10 Brucella abortus strains isolated from water buffaloes farmed in the Campania region of Italy.
April 19, 2018: Genome Announcements
M D Givens
The purpose of this paper is to review scientific evidence concerning pathogens that could potentially be transmitted via bovine semen. As a result of a careful analysis of the characteristics of infections that may cause transmission of disease through semen, effective control procedures can be identified that provide minimal constraint to the introduction of new bulls into herds for natural breeding and importation of valuable novel genetics through artificial insemination. The potential for transmission through bovine semen and corresponding effective control procedures are described for bovine herpesvirus 1, bovine viral diarrhea virus, bovine leukemia virus, lumpy skin disease virus, bluetongue virus, foot-and-mouth disease virus, and Schmallenberg virus...
April 18, 2018: Animal: An International Journal of Animal Bioscience
Soumya Paul, Bhavani V Peddayelachagiri, Sowmya Nagaraj, Joseph J Kingston, Harsh V Batra
Development of a safe and efficacious vaccine for brucellosis is a long standing challenge for scientists. Recognizing potential antigens towards developing vaccine candidate is crucial. Omp25c, a porin protein of Brucella, is a paralog of two previously identified promising vaccine candidates namely, Omp25 and Omp31, with notable sequence identity. Also, Omp25c is conserved in all major Brucella species. This highlights the possibility of employing this protein in multivalent subunit vaccine based approach of Brucella management...
April 9, 2018: Molecular Immunology
Qian Wang, Shanshan Zhao, Hazi Wureli, Songsong Xie, Chuangfu Chen, Qingqing Wei, Buyun Cui, Changchun Tu, Yuanzhi Wang
Brucellosis is a relatively common zoonosis, particularly in the developing countries. Our aim was to cultivate the Brucella wild-type strain from eggs of Dermacentor marginatus, and to molecularly investigate the presence of Brucella DNA in different developmental stages. A total of 350 engorged adult female ticks were collected from sheep and cattle, and individually placed into a single clean breathable tube suitable for laying eggs and for the development of larvae. Detection of Brucella DNA was attempted in engorged female ticks and their offsprings based on the Brucella outer membrane protein gene 22 (omp22) and IS711 gene...
March 26, 2018: Ticks and Tick-borne Diseases
Siobhan M Mor, Anke K Wiethoelter, Peter D Massey, Jennifer Robson, Kathryn Wilks, Penny Hutchinson
BACKGROUND: Brucellosis, also known as undulant, Mediterranean or Malta fever, is a systemic infection that causes fever, sweats, arthralgias and myalgias. A globally important disease, brucellosis is re-emerging in Australia in association with feral pig hunting activities. OBJECTIVE: This article aims to provide clinicians with an overview of brucellosis, covering epidemiology, clinical features, diagnosis, management and prevention. DISCUSSION: Brucellosis should be suspected in all patients with non-specific, flu-like illness who fall into one of the major risk groups (feral pig hunters, overseas travellers and migrants)...
March 2018: Australian journal of general practice
Gholamhassan Azimi, Ali Hakakian, Mustafa Ghanadian, Ali Joumaa, Saeed Alamian
Berberis integerrima Bonge. (Syn: Berberis densiflora Boiss. & Buhse) is a shrub widely distributed in Middle East and central part of Asia. An ethnobotanical study revealed that indigenous and tribal people in Iran use B. integerrima root decoction for treatment of brucellosis. Therefore, the aim of this study was bioassay directed isolation of antibacterial compounds from this plant based on their in vitro bactericidal activity against Brucella abortus . Briefly, the ethanol extract of B. integerrima was fractioned and subjected to preliminary antibacterial screening tests against Brucella...
April 2018: Research in Pharmaceutical Sciences
Rubina Paradiso, Massimiliano Orsini, Marita Georgia Riccardi, Bianca Cecere, Anna Cerrone, Cesare Cammà, Maria Luisa Chiusano, Giorgio Galiero, Giorgia Borriello
Brucellosis is a zoonotic disease caused by bacteria of the genus Brucella The disease is endemic in many areas, causing chronic infections responsible for reproductive disorders in infected animals. Here, we present eight complete genome assemblies of eight Brucella abortus strains isolated from water buffaloes farmed in the Campania region.
March 29, 2018: Genome Announcements
Mingxing Tian, Yanqing Bao, Peng Li, Hai Hu, Chan Ding, Shaohui Wang, Tao Li, Jingjing Qi, Xiaolan Wang, Shengqing Yu
Brucellosis is a zoonotic bacterial disease caused by Brucella spp. The virulence of these bacteria is dependent on their ability to invade and replicate within host cells. In a previous study, a putative gene bab_RS27735 encoding an amino acid ABC transporter substrate-binding protein homologous to AapJ protein was found to be involved in Brucella abortus virulence. In this study, we successfully constructed a bab_RS27735 deletion mutant, Δ27735. Compared with the wild-type strain, the lipopolysaccharide pattern of the mutant was not changed, but the growth ability was slightly defected in the exponential phase...
March 29, 2018: Veterinary Research
Elodie Lobet, Kevin Willemart, Noëlle Ninane, Catherine Demazy, Jaroslaw Sedzicki, Christophe Lelubre, Xavier De Bolle, Patricia Renard, Martine Raes, Christoph Dehio, Jean-Jacques Letesson, Thierry Arnould
Mitochondria are complex organelles that participate in many cellular functions, ranging from ATP production to immune responses against viruses and bacteria. This integration of a plethora of functions within a single organelle makes mitochondria a very attractive target to manipulate for intracellular pathogens. We characterised the crosstalk that exists between Brucella abortus, the causative agent of brucellosis, and the mitochondria of infected cells. Brucella replicates in a compartment derived from the endoplasmic reticulum (ER) and modulates ER functionality by activating the unfolded protein response...
March 26, 2018: Scientific Reports
Hossin Ali Rahdar, Reza Golmohammadi, Reza Mirnejad, Ramezan Ali Ataee, Gholam Hossein Alishiri, Hossein Kazemian
The presence of Brucella melitensis and Brucella abortus genomes were investigated in the synovial fluid (SF) samples from 90 patients with rheumatoid arthritis (RA). DNA extraction and PCR assay were performed for simultaneous identification and discrimination of B. melitensis and B. abortus from the SF using three specific primers. After gel electrophoresis, the PCR products were confirmed by DNA sequencing. The cbg, omp31, manA, virB, and znuA virulence genes typing were performed by multiplex-PCR. Of the 90 samples, 14 were positive for B...
March 22, 2018: Microbial Pathogenesis
Julien Herrou, Daniel M Czyż, Aretha Fiebig, Jonathan W Willett, Youngchang Kim, Ruiying Wu, Gyorgy Babnigg, Sean Crosson
The general stress response sigma factor σE1 directly and indirectly regulates the transcription of dozens of genes that influence stress survival and host infection in the zoonotic pathogen Brucella abortus Characterizing the functions of σE1 -regulated genes therefore would contribute to our understanding of B. abortus physiology and infection biology. σE1 indirectly activates transcription of the IclR family regulator Bab2_0215, but the function of this regulator remains undefined. Here, we present a structural and functional characterization of Bab2_0215, which we have named <u>B</u> rucella <u>a</u>dipic<u>a</u>cid-activated<u>r</u>egulator (BaaR)...
March 22, 2018: Journal of Biological Chemistry
Morteza Abkar, Mahdi Fasihi-Ramandi, Hamid Kooshki, Abbas Sahebghadam Lotfi
Brucella (B) species are brucellosis causative agents, a worldwide zoonotic illness causing Malta fever in humans and abortion in domestic animals. In this work, we evaluated the vaccine potential of Trimethyl chitosan (TMC) nanoparticles formulation of Urease (TMC/Urease) against brucellosis. TMC/Urease nanoparticles and urease without any adjuvant were separately administered both orally and intraperitoneally. Intraperitoneal (i.p.) administration of urease alone as well as oral administration of both TMC/Urease and urease alone, elicited low titers of specific immunoglobulin G (IgG), while i...
March 13, 2018: Immunology Letters
Guirong Tang, Qiong Li, Shenghui Xing, Ningning Li, Zheng Tang, Liangliang Yu, Junhui Yan, Xuan Li, Li Luo
Agrobacterium tumefaciens infects and causes crown galls in dicot plants by transferring T-DNA from the Ti plasmid to the host plant via a type IV secretion system (T4SS). This process requires appropriate environmental conditions, certain plant secretions and bacterial regulators. In our previous work, a member of the LysR family of transcriptional regulators (LsrB) in Sinorhizobium meliloti was found to modulate its symbiotic interactions with the host plant alfalfa. However, the function of its homologue in A...
March 16, 2018: Molecular Plant-microbe Interactions: MPMI
Maroun M Sfeir
Brucellosis is a challenging zoonosis to diagnose and treat. The recent outbreaks of Brucella abortus RB51 in several states in the United States, including New Jersey and Texas, due to raw milk consumption has raised the concern of drug-resistant Brucella spp. This commentary highlights the importance of being on the lookout for this emerging infection.
March 14, 2018: Journal of Medical Microbiology
Maryam Golshani, Melina Ghasemian, Nematollah Gheibi, Saeid Bouzari
Background: L7/L12 is a protective antigen conserved in main Brucella pathogens and is considered as potential vaccine candidate. Outer membrane protein 2b is an immunogen conserved in all Brucella pathogens. Materials and Methods: The purpose of the current study was to in silico design a L7/L12-SOmp2b fusion protein and in vitro production of the chimera. Two possible fusion forms, L7/L12-SOmp2b and SOmp2b-L7/L12, were subjected to in silico modeling and analysis...
2018: Advanced Biomedical Research
Gavin G Cotterill, Paul C Cross, Eric K Cole, Rebecca K Fuda, Jared D Rogerson, Brandon M Scurlock, Johan T du Toit
Providing food to wildlife during periods when natural food is limited results in aggregations that may facilitate disease transmission. This is exemplified in western Wyoming where institutional feeding over the past century has aimed to mitigate wildlife-livestock conflict and minimize winter mortality of elk ( Cervus canadensis ). Here we review research across 23 winter feedgrounds where the most studied disease is brucellosis, caused by the bacterium Brucella abortus Traditional veterinary practices (vaccination, test-and-slaughter) have thus far been unable to control this disease in elk, which can spill over to cattle...
May 5, 2018: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
Mohamed El-Diasty, Gamal Wareth, Falk Melzer, Shawky Mustafa, Lisa D Sprague, Heinrich Neubauer
Brucellosis is a zoonosis occurring worldwide, with economic and public health impacts. Its diagnosis remains a challenge in endemic countries and basically relies on serology. The present study was carried out on two dairy cattle farms allegedly free from brucellosis, but with sporadic cases of abortion. The aim of this study was to investigate the presence of Brucella (B.) spp. in uterine discharge of seronegative cows after abortion. In farm I, B. melitensis biovar (bv) 3 was cultured from two of five cows after abortion, while in farm II, B...
March 9, 2018: Veterinary Sciences
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