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Brucella abortus

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https://www.readbyqxmd.com/read/28535754/a-comprehensive-proteogenomic-study-of-the-human-brucella-vaccine-strain-104%C3%A2-m
#1
Xiaodong Zai, Qiaoling Yang, Kun Liu, Ruihua Li, Mengying Qian, Taoran Zhao, Yaohui Li, Ying Yin, Dayong Dong, Ling Fu, Shanhu Li, Junjie Xu, Wei Chen
BACKGROUND: Brucella spp. are Gram-negative, facultative intracellular pathogens that cause brucellosis in both humans and animals. The B. abortus vaccine strain 104 M is the only vaccine available in China for the prevention of brucellosis in humans. Although the B. abortus 104 M genome has been fully sequenced, the current genome annotations are not yet complete. In addition, the main mechanisms underpinning its residual toxicity and vaccine-induced immune protection have yet to be elucidated...
May 23, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28533142/evaluation-of-plasma-sphingosine-1-phosphate-hepcidin-and-cardiovascular-damage-biomarkers-cardiac-troponin-i-and-homocysteine-in-rats-infected-with-brucellosis-and-vaccinated-rev-1-rb-51
#2
Kaveh Azimzadeh, Reza Nasrollahi Nargesabad, Nasim Vousooghi
Brucellosis is known as one of important zoonosis. Studying the histological and biochemical effects of the disease could help to increase our knowledge about it. The aim of the present study was to evaluate changes of plasma parameters after intraperitoneal injection of two species of Brucella (Brucella melitensis and Brucella abortus) and two vaccines (Rev-1, RB-51) in the rat. Forty male rats were divided into five groups (n = 8 in each group). Two groups received suspensions of Brucella abortus and Brucella melitensis and two other groups were injected intraperitoneally with two mentioned vaccines and the last group received only distilled water...
May 19, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28529959/getting-inside-type-i-ifns-type-i-ifns-in-intracellular-bacterial-infections
#3
REVIEW
Deann T Snyder, Jodi F Hedges, Mark A Jutila
Type I interferons represent a unique and complex group of cytokines, serving many purposes during innate and adaptive immunity. Discovered in the context of viral infections, type I IFNs are now known to have myriad effects in infectious and autoimmune disease settings. Type I IFN signaling during bacterial infections is dependent on many factors including whether the infecting bacterium is intracellular or extracellular, as different signaling pathways are activated. As such, the repercussions of type I IFN induction can positively or negatively impact the disease outcome...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28508154/estimating-loss-of-brucella-abortus-antibodies-from-age-specific-serological-data-in-elk
#4
J A Benavides, D Caillaud, B M Scurlock, E J Maichak, W H Edwards, P C Cross
Serological data are one of the primary sources of information for disease monitoring in wildlife. However, the duration of the seropositive status of exposed individuals is almost always unknown for many free-ranging host species. Directly estimating rates of antibody loss typically requires difficult longitudinal sampling of individuals following seroconversion. Instead, we propose a Bayesian statistical approach linking age and serological data to a mechanistic epidemiological model to infer brucellosis infection, the probability of antibody loss, and recovery rates of elk (Cervus canadensis) in the Greater Yellowstone Ecosystem...
May 15, 2017: EcoHealth
https://www.readbyqxmd.com/read/28506887/molecular-investigation-of-virulence-factors-of-brucella-melitensis-and-brucella-abortus-strains-isolated-from-clinical-and-non-clinical-samples
#5
Reza Mirnejad, Faramarz Masjedian Jazi, Shayan Mostafaei, Mansour Sedighi
Brucella is zoonotic pathogen that induces abortion and sterility in domestic mammals and chronic infections in humans called Malta fever. It is a facultative intracellular potential pathogen with high infectivity. The virulence of Brucella is dependent upon its potential virulence factors such as enzymes and cell envelope associated virulence genes. The aim of this study was to investigate the Brucella virulence factors among strains isolated from humans and animals in different parts of Iran. Seventy eight strains of Brucella species isolated from suspected human and animal cases from several provinces of Iran during 2015-2016 and identified by phenotypic and molecular methods...
May 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28506316/characterization-of-novel-omp31-antigenic-epitopes-of-brucella-melitensis-by-monoclonal-antibodies
#6
Jinfeng Li, Feihuan Hu, Shouyi Chen, Peifang Luo, Zuoping He, Wenjing Wang, Jean-Pierre Allain, Chengyao Li
BACKGROUND: Brucellosis is a severe zoonotic disease worldwide. Detection and identification of Brucella species are essential to prevent or treat brucellosis in humans and animals. The outer membrane protein-31 (Omp31) is a major protein of Brucellae except for B. abortus, while the Omp31 antigenic epitopes have not been extensively characterized yet. RESULTS: A total of 22 monoclonal antibodies (mAbs) were produced against Omp31 of Brucella (B.) melitensis, of which 13 recognized five linear epitopes, 7 reacted with semi-conformational epitopes and 2 reacted with conformational epitopes, respectively...
May 15, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28466240/incidence-and-knowledge-of-bovine-brucellosis-in-kahuro-district-murang-a-county-kenya
#7
Janeffer N Njuguna, Michael M Gicheru, Lucy M Kamau, Peter M Mbatha
Brucellosis in cattle is a zoonosis mainly caused by Brucella abortus. In Kenya, the disease is widespread, but its prevalence is largely unknown. The objective of this study was to investigate incidence rates of brucellosis and farmers' knowledge on the disease in Kahuro district, Murang'a County. In this study, 150 pooled milk samples were collected from 75 milk collection centers and tested. Subsequently, 230 milk samples were collected from farmers in 16 collection centers in Wangu and Mugoiri divisions whose pooled samples gave positive results...
May 3, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28460642/multi-locus-variable-number-tandem-repeat-analysis-of-chinese-brucella-strains-isolated-from-1953-to-2013
#8
Guo-Zhong Tian, Bu-Yun Cui, Dong-Ri Piao, Hong-Yan Zhao, Lan-Yu Li, Xi Liu, Pei Xiao, Zhong-Zhi Zhao, Li-Qing Xu, Hai Jiang, Zhen-Jun Li
BACKGROUND: Brucellosis was a common human and livestock disease caused by Brucella strains, the category B priority pathogens by the US Center for Disease Control (CDC). Identified as a priority disease in human and livestock populations, the increasing incidence in recent years in China needs urgent control measures for this disease but the molecular background important for monitoring the epidemiology of Brucella strains at the national level is still lacking. METHODS: A total of 600 Brucella isolates collected during 60 years (from 1953 to 2013) in China were genotyped by multiple locus variable-number tandem repeat analysis (MLVA) and the variation degree of MLVA11 loci was calculated by the Hunter Gaston Diversity Index (HGDI) values...
May 2, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28452907/in-a-mouse-model-of-sepsis-hepcidin-ablation-ameliorates-anemia-more-effectively-than-iron-and-erythropoietin-treatment
#9
Eeman Khorramian, Eileen Fung, Kristine Chua, Victoria Gabayan, Tomas Ganz, Elizabeta Nemeth, Airie Kim
Intensive Care Unit (ICU) anemia is an extreme version of anemia of inflammation (AI) that occurs commonly in critically ill patients and is associated with increased morbidity and mortality. Currently available therapies for ICU anemia have shown inconsistent efficacies in clinical trials. We conducted a systematic study of the effects of early versus delayed iron (Fe) and/or erythropoietin (EPO) therapy in our previously characterized mouse model of ICU anemia based on an injection of heat-killed Brucella abortus (BA)...
April 27, 2017: Shock
https://www.readbyqxmd.com/read/28443226/a-novel-pcr-assay-for-detecting-brucella-abortus-and-brucella-melitensis
#10
Saeed Alamian, Majid Esmaelizad, Taghi Zahraei, Afshar Etemadi, Mohsen Mohammadi, Davoud Afshar, Soheila Ghaderi
OBJECTIVES: Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis. METHODS: All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all available Brucella sequences. A unique repeat sequence (URS) locus on chromosome 1 could differentiate Brucella abortus from Brucella melitensis...
February 2017: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/28398652/brucella-abortus-activated-microglia-induce-neuronal-death-through-primary-phagocytosis
#11
Ana M Rodríguez, M Victoria Delpino, M Cruz Miraglia, Miriam M Costa Franco, Paula Barrionuevo, Vida A Dennis, Sergio C Oliveira, Guillermo H Giambartolomei
Inflammation has long been implicated as a contributor to pathogenesis in neurobrucellosis. Many of the associated neurocognitive symptoms of neurobrucellosis may be the result of neuronal dysfunction resulting from the inflammatory response induced by Brucella abortus infection in the central nervous system. In this manuscript, we describe an immune mechanism for inflammatory activation of microglia that leads to neuronal death upon B. abortus infection. B. abortus was unable to infect or harm primary cultures of mouse neurons...
April 11, 2017: Glia
https://www.readbyqxmd.com/read/28391992/protein-protein-dna-dna-and-dna-protein-based-vaccination-strategies-using-truncated-omp2b-against-brucella-infection-in-balb-c-mice
#12
Maryam Golshani, Sima Rafati, Mehdi Nejati-Moheimani, Somaye Pourabdi, Amin Arsang, Saeid Bouzari
The purpose of the present study was to evaluate the immunogenicity and protective efficacy of the truncated form of outer membrane protein 2b (TOmp2b) from Brucella abortus in BALB/c mice. Three immunization regimens Protein/Protein, DNA/DNA and DNA/Protein were used. Immunization of mice with all vaccine strategies elicited a strong specific IgG responses (IgG2a titers over IgG1) and provided T helper1 (Th1) oriented immune responses. Furthermore, Protein/Protein (Pro/Pro-) and DNA/Pro- vaccinated groups conferred protection levels against B...
March 28, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28376905/characterization-of-brucella-abortus-mutant-strain-%C3%AE-22915-a-potential-vaccine-candidate
#13
Yanqing Bao, Mingxing Tian, Peng Li, Jiameng Liu, Chan Ding, Shengqing Yu
Brucellosis, caused by Brucella spp., is an important zoonosis worldwide. Vaccination is an effective strategy for protection against Brucella infection in livestock in developing countries and in wildlife in developed countries. However, current vaccine strains including S19 and RB51 are pathogenic to humans and pregnant animals, limiting their use. In this study, we constructed the Brucella abortus (B. abortus) S2308 mutant strain Δ22915, in which the putative lytic transglycosylase gene BAB_RS22915 was deleted...
April 4, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28296882/detection-and-characterization-of-brucella-spp-in-bovine-milk-in-small-scale-urban-and-peri-urban-farming-in-tajikistan
#14
Elisabeth Lindahl-Rajala, Tove Hoffman, David Fretin, Jacques Godfroid, Nosirjon Sattorov, Sofia Boqvist, Åke Lundkvist, Ulf Magnusson
Brucellosis is one of the most common zoonoses globally, and Central Asia remains a Brucella hotspot. The World Health Organization classifies brucellosis as a neglected zoonotic disease that is rarely in the spotlight for research and mainly affects poor, marginalized people. Urban and peri-urban farming is a common practice in many low-income countries, and it increases the incomes of families that are often restrained by limited economic resources. However, there is a concern that the growing number of people and livestock living close together in these areas will increase the transmission of zoonotic pathogens such as Brucella...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28284850/molecular-prevalence-of-putative-virulence-associated-genes-in-brucella-melitensis-and-brucella-abortus-isolates-from-human-and-livestock-specimens-in-iran
#15
Iman Hashemifar, Abbas Yadegar, Faramarz Masjedian Jazi, Nour Amirmozafari
Molecular prevalence of nine putative virulence factors in two more prevalent Brucella species in Iranian patients and livestock was investigated. During five years (2010-2015), 120 human and animal specimens were collected from three geographical areas of Iran. All samples were cultured in blood culture media and subcultured into Brucella agar medium. Nine primer pairs were designed for detection of VirB2, VirB5, VceC, BtpA, BtpB, PrpA, BetB, BPE275 and BSPB virulence factors using PCR and sequence analysis...
April 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28281771/an-evaluation-of-serological-tests-in-the-diagnosis-of-bovine-brucellosis-in-naturally-infected-cattle-in-kwazulu-natal-province-in-south-africa
#16
Songelwayo L Chisi, Yoanda Marageni, Prebashni Naidoo, Gloria Zulu, George W Akol, Henriette Van Heerden
The diagnostic sensitivity (DSe) of the Rose Bengal test (RBT), the complement fixation test (CFT), the serum agglutination test (SAT), the competitive enzyme-linked immunosorbent assay (cELISA) and the indirect ELISA (iELISA) were determined in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture (gold standard). Natural brucellosis infection status of animals was determined by culturing and identification of Brucella abortus biovar 1 from abomasal fluid, milk, hygroma fluid, lymph nodes or uterine discharges samples...
February 28, 2017: Journal of the South African Veterinary Association
https://www.readbyqxmd.com/read/28265268/brucella-and-osteoarticular-cell-activation-partners-in-crime
#17
REVIEW
Guillermo H Giambartolomei, Paula C Arriola Benitez, M Victoria Delpino
Osteoarticular brucellosis is the most common presentation of human active disease although its prevalence varies widely. The three most common forms of osteoarticular involvement are sacroiliitis, spondylitis, and peripheral arthritis. The molecular mechanisms implicated in bone damage have been recently elucidated. B. abortus induces bone damage through diverse mechanisms in which TNF-α and the receptor activator of nuclear factor kappa-B ligand (RANKL)-the natural modulator of bone homeostasis are involved...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28264909/promotion-and-rescue-of-intracellular-brucella-neotomae-replication-during-coinfection-with-legionella-pneumophila
#18
Yoon-Suk Kang, James E Kirby
We established a new Brucella neotomaein vitro model system for study of type IV secretion system-dependent (T4SS) pathogenesis in the Brucella genus. Importantly, B. neotomae is a rodent pathogen, and unlike B. abortus, B. melitensis, and B. suis, B. neotomae has not been observed to infect humans. It therefore can be handled more facilely using biosafety level 2 practices. More particularly, using a series of novel fluorescent protein and lux operon reporter systems to differentially label pathogens and track intracellular replication, we confirmed T4SS-dependent intracellular growth of B...
May 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28262966/serologic-evidence-of-brucella-infection-in-pinnipeds-along-the-coast-of-hokkaido-the-northernmost-main-island-of-japan
#19
Erika Abe, Kazue Ohishi, Tsuyoshi Ishinazaka, Kei Fujii, Tadashi Maruyama
Brucella infection in Hokkaido was serologically surveyed in four species of pinnipeds inhabiting Cape Erimo during 2008-2013 and the Shiretoko Peninsula in 1999 by ELISA using Brucella abortus and B. canis as antigens. Anti-Brucella positive sera showed higher absorbance to B. abortus than B. canis in almost all samples. Anti-B. abortus antibodies were detected in serum samples from 24% (n = 55) of Western Pacific harbor seals (Phoca vitulina stejnegeri) in Cape Erimo and from 66% (n = 41) of spotted seals (P...
April 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28261222/u-omp19-from-brucella-abortus-is-a-useful-adjuvant-for-vaccine-formulations-against-salmonella-infection-in-mice
#20
Gabriela S Risso, Marianela V Carabajal, Laura A Bruno, Andrés E Ibañez, Lorena M Coria, Karina A Pasquevich, Seung-Joo Lee, Stephen J McSorley, Gabriel Briones, Juliana Cassataro
Most pathogens infect through mucosal surfaces, and parenteral immunization typically fails to induce effective immune responses at these sites. Development of oral-administered vaccines capable of inducing mucosal as well as systemic immunity while bypassing the issues of antigen degradation and immune tolerance could be crucial for the control of enteropathogens. This study demonstrates that U-Omp19, a bacterial protease inhibitor with immunostimulatory features, coadministered with Salmonella antigens by the oral route, enhances mucosal and systemic immune responses in mice...
2017: Frontiers in Immunology
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