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Brucella abortus

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https://www.readbyqxmd.com/read/29203548/utilization-of-host-polyamines-in-alternatively-activated-macrophages-promotes-chronic-infection-by-brucella-abortus
#1
Tobias Kerrinnes, Maria G Winter, Briana M Young, Vladimir E Diaz-Ochoa, Sebastian E Winter, Renée M Tsolis
Treatment of intracellular bacterial pathogens with antibiotic therapy often requires a long course of multiple antibiotics. A barrier to developing strategies that enhance antibiotic efficacy against these pathogens is our poor understanding of the intracellular nutritional environment that maintains bacterial persistence. The intracellular pathogen Brucella abortus survives and replicates preferentially in alternatively activated macrophages (AAM), however knowledge of the metabolic adaptations promoting exploitation of this niche is limited...
December 4, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29203515/brucella-abortus-triggers-a-cgas-independent-sting-pathway-to-induce-host-protection-that-involves-guanylate-binding-proteins-and-inflammasome-activation
#2
Miriam M Costa Franco, Fernanda Marim, Erika S Guimarães, Natan R G Assis, Daiane M Cerqueira, Juliana Alves-Silva, Jerome Harms, Gary Splitter, Judith Smith, Thirumala-Devi Kanneganti, Nina M G P de Queiroz, Delia Gutman, Glen N Barber, Sergio C Oliveira
Immunity against microbes depends on recognition of pathogen-associated molecular patterns by innate receptors. Signaling pathways triggered by Brucella abortus DNA involves TLR9, AIM2, and stimulator of IFN genes (STING). In this study, we observed by microarray analysis that several type I IFN-associated genes, such as IFN-β and guanylate-binding proteins (GBPs), are downregulated in STING knockout (KO) macrophages infected with Brucella or transfected with DNA. Additionally, we determined that STING and cyclic GMP-AMP synthase (cGAS) are important to engage the type I IFN pathway, but only STING is required to induce IL-1β secretion, caspase-1 activation, and GBP2 and GBP3 expression...
December 4, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29193808/molecular-epidemiological-characterization-of-brucella-isolates-from-sheep-and-yaks-in-northwest-china
#3
X Cao, Z Li, Z Liu, B Fu, Y Liu, Y Shang, J Zhou, Z Jing
Animal brucellosis is a re-emerging disease in China with high prevalence in the northwest region. A total of 66 isolates of Brucella were recovered from sheep and yaks in the Inner Mongolia, Xinjiang, Qinghai and Gansu provinces of northwest China in 2015 and 2016. Using classical biotyping and the Brucella AMOS PCR assay, all isolates were identified as Brucella melitensis biovar 3 (n = 58), B. melitensis biovar 1 (n = 1), Brucella abortus (n = 5), or Brucella suis biovar 3 (n = 2), and B. melitensis biovar 3 was found to be mainly responsible for sheep brucellosis in northwest China...
November 29, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29187839/the-glutaminase-dependent-system-confers-extreme-acid-resistance-to-new-species-and-atypical-strains-of-brucella
#4
Luca Freddi, Maria A Damiano, Laurent Chaloin, Eugenia Pennacchietti, Sascha Al Dahouk, Stephan Köhler, Daniela De Biase, Alessandra Occhialini
Neutralophilic bacteria have developed specific mechanisms to cope with the acid stress encountered in environments such as soil, fermented foods, and host compartments. In Escherichia coli, the glutamate decarboxylase (Gad)-dependent system is extremely efficient: it requires the concerted action of glutamate decarboxylase (GadA/GadB) and of the glutamate (Glu)/γ-aminobutyrate antiporter, GadC. Notably, this system is operative also in new strains/species of Brucella, among which Brucella microti, but not in the "classical" species, with the exception of marine mammals strains...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29184543/modulation-of-microtubule-dynamics-affects-brucella-abortus-intracellular-survival-pathogen-containing-vacuole-maturation-and-pro-inflammatory-cytokine-production-in-infected-macrophages
#5
Juliana Alves-Silva, Isabela P Tavares, Erika S Guimarães, Miriam M Costa Franco, Barbara C Figueiredo, João T Marques, Gary Splitter, Sergio C Oliveira
The microtubule (MT) cytoskeleton regulates several cellular processes related to the immune system. For instance, an intricate intracellular transport mediated by MTs is responsible for the proper localization of vesicular receptors of innate immunity and its adaptor proteins. In the present study, we used nocodazole to induce MTs depolymerization and paclitaxel or recombinant (r) TIR (Toll/interleukin-1 receptor) domain containing protein (TcpB) to induce MT stabilization in bone marrow-derived macrophages infected with Brucella abortus...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29178956/brucella-seroprevalence-in-cattle-near-a-wildlife-reserve-in-kenya
#6
Sofie Enström, Daniel Nthiwa, Bernard Bett, Amanda Karlsson, Silvia Alonso, Johanna F Lindahl
OBJECTIVES: Brucellosis is caused by bacteria from the genus Brucella which infect human and domestic animals as well as wildlife. The Maasai Mara National Reserve has vast populations of wild ruminants such as buffaloes and wildebeest which could contribute to the risk of brucellosis in livestock, and the surrounding pastoralist communities grazing cattle in and around the reserve may be exposed to a higher risk of zoonotic diseases like brucellosis due to the close contact with livestock...
November 25, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29173617/identification-of-brucella-genus-and-eight-brucella-species-by-luminex-bead-based-suspension-array
#7
Tina S Lusk Pfefer, Ruth Timme, Julie A Kase
Globally, unpasteurized milk products are vehicles for the transmission of brucellosis, a zoonosis responsible for cases of foodborne illness in the United States and elsewhere. Existing PCR assays to detect Brucella species are restricted by the resolution of band sizes on a gel or the number of fluorescent channels in a single real-time system. The Luminex bead-based suspension array is performed in a 96-well plate allowing for high throughput screening of up to 100 targets in one sample with easily discernible results...
April 2018: Food Microbiology
https://www.readbyqxmd.com/read/29168343/lipocalin-2-lcn2-interferes-with-iron-uptake-by-brucella-abortus-and-dampens-immunoregulation-during-infection-of-raw-264-7-macrophages
#8
Huynh Tan Hop, Lauren Togonon Arayan, Tran Xuan Ngoc Huy, Alisha Wehdnesday Bernardo Reyes, Eun Jin Baek, WonGi Min, Hu Jang Lee, Man Hee Rhee, Kenta Watanabe, Hong Hee Chang, Suk Kim
Lipocalin 2 (Lcn2) is an important innate immunity component against bacterial pathogens. In this study, we report that Lcn2 is induced by Brucella (B.) abortus infection and significantly contributes to the restriction of intracellular survival of Brucella in macrophages. We found that Lcn2 prevented iron uptake by B. abortus through two distinct mechanisms. First, Lcn2 is secreted to capture bacterial siderophore(s) and abrogate iron import by Brucella. Second, Lcn2 decreases the intracellular iron levels during Brucella infection, which probably deprives the invading Brucella of the iron source needed for growth...
November 22, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/29163647/comparison-of-diagnostic-tests-for-the-detection-of-bovine-brucellosis-in-the-natural-cases-of-abortion
#9
V Mahajan, H S Banga, G Filia, M P Gupta, K Gupta
Rapid and precise diagnosis in natural field cases of bovine abortion caused by Brucella abortus warrants the use of the most sensitive and reliable diagnostic methods. In the present study, bacterial isolation, serology, gross, histopathology, immunohistochemistry and polymerase chain reaction technique(s) were applied. Sero-prevalence studies showed the rate of 28.86% positive cases using the competitive ELISA. Histopathological changes were mainly seen in the placenta, fetal lungs, kidney, liver and spleen...
2017: Iranian Journal of Veterinary Research
https://www.readbyqxmd.com/read/29154929/identification-of-genetic-variants-of-brucella-spp-through-genome-wide-association-studies
#10
Jagadesan Sankarasubramanian, Udayakumar S Vishnu, Paramasamy Gunasekaran, Jeyaprakash Rajendhran
Brucellosis is an important zoonotic disease caused by Brucella spp. We present a phylogeny of 552 strains based on genome-wide single nucleotide polymorphisms (SNPs) determined by an alignment-free k-mer approach. A total of 138,029 SNPs were identified from 552 Brucella genomes. Of these, 31,152 and 106,877 were core and non-core SNPs, respectively. Based on pan-genome analysis 11,937 and 972 genes were identified as pan and core genome, respectively. The pan-genome-wide analysis studies (Pan-GWAS) could not identify the group-specific variants in Brucella spp...
November 15, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29150637/ml-proteins-from-mesorhizobium-loti-and-mucr-from-brucella-abortus-an-at-rich-core-dna-target-site-and-oligomerization-ability
#11
Ilaria Baglivo, Luciano Pirone, Emilia Maria Pedone, Joshua Edison Pitzer, Lidia Muscariello, Maria Michela Marino, Gaetano Malgieri, Andrea Freschi, Angela Chambery, Roy-Martin Roop Ii, Paolo Vincenzo Pedone
Mesorhizobium loti contains ten genes coding for proteins sharing high amino acid sequence identity with members of the Ros/MucR transcription factor family. Five of these Ros/MucR family members from Mesorhizobium loti (Ml proteins) have been recently structurally and functionally characterized demonstrating that Ml proteins are DNA-binding proteins. However, the DNA-binding studies were performed using the Ros DNA-binding site with the Ml proteins. Currently, there is no evidence as to when the Ml proteins are expressed during the Mesorhizobium lo ti life cycle as well as no information concerning their natural DNA-binding site...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29106986/intraperitoneal-administration-of-a-novel-chimeric-immunogen-rop-elicits-ifn-%C3%AE-and-il-12p70-protective-immune-response-in-balb-c-mice-against-virulent-brucella
#12
Ganesh Tadepalli, Balakrishna Konduru, Harishchandra Sripathy Murali, Harsh Vardhan Batra
Recombinant engineering of immunologically active chimeric protein consisting of Omp19 and P39 domains of B. abortus (rOP), was purified under denaturing conditions upon expression in E. coli BL21 (DE3) and refolded to dynamic form. The immuno-protective efficacy of rOP was evaluated by challenging the BALB/c mice intraperitoneally (I.P) with the infective species of Brucella in the absence or presence of adjuvants, such as Aluminum hydroxide gel (Al), or Freund's Complete Adjuvant (FCA)/Incomplete Freund's Adjuvant (IFA)...
October 26, 2017: Immunology Letters
https://www.readbyqxmd.com/read/29102107/the-characterization-of-brucella-strains-isolated-from-cattle-in-algeria-reveals-the-existence-of-a-b-abortus-lineage-distinct-from-european-and-sub-saharan-africa-strains
#13
Mammar Khames, Virginie Mick, M Jesús de Miguel, Guillaume Girault, Raquel Conde-Álvarez, Djamel Khelef, Mustapha Oumouna, Ignacio Moriyón, Pilar M Muñoz, Amaia Zúñiga-Ripa
Brucellosis is a zoonosis caused by bacteria of the genus Brucella that causes important economic losses and human suffering worldwide. Brucellosis control requires an understanding of the Brucella species circulating in livestock and humans and, although prevalent in African countries of the Mediterranean basin, data for this area are mostly restricted to isolates obtained from humans and small ruminants. Here, we report the characterization of twenty-four Brucella strains isolated from Algerian cattle. Bruce-ladder multiplex PCR and conventional biotyping showed that Algerian cattle are infected mostly by B...
November 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/29082458/high-level-of-b19-strain-detection-in-brazilian-cattle-semen
#14
M P S Lourencetti, M A Souza, M R Ganda, J P Santos, A Ferreira Júnior, S Miyashiro, A M C Lima
Semen contaminated with microorganisms can disseminate serious diseases including brucellosis. The objectives of this study were to detect Brucella-specific antibodies and Brucella abortus DNA in samples of blood and fresh semen from 100 animals older than 20 months. The samples were collected on farms and in semen collection and processing centers (CCPS). The serum samples were evaluated by Rose Bengal test (RBT). B. abortus DNA was detected by a polymerase chain reaction (PCR) using BAB and IS771 primers...
October 29, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29082252/multivalent-fusion-dna-vaccine-against-brucella-abortus
#15
Leonardo Gómez, Javiera Llanos, Emilia Escalona, Darwin Sáez, Francisco Álvarez, Raúl Molina, Manuel Flores, Angel Oñate
As an alternative brucellosis prevention method, we evaluated the immunogenicity induced by new multivalent DNA vaccines in BALB/c mice. We constructed the vaccines by fusion of BAB1_0273 and/or BAB1_0278 open reading frames (ORFs) from genomic island 3 (GI-3) and the Brucella abortus 2308 sodC gene with a link based on prolines and alanines (pV273-sod, pV278-sod, and pV273-278-sod, resp.). Results show that immunization with all tested multivalent DNA vaccines induced a specific humoral and cellular immune response...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29076560/an-account-of-evolutionary-specialization-the-abcr-small-rnas-in-the-rhizobiales
#16
REVIEW
Lauren M Sheehan, Clayton C Caswell
The AbcR small RNAs (sRNAs) are a fascinating example of two highly conserved sRNAs that differ tremendously at the functional level amongst organisms. From their transcriptional activation to their regulatory capabilities, the AbcR sRNAs exhibit varying characteristics in three well-studied bacteria belonging to the Rhizobiales order: the plant symbiont Sinorhizobium meliloti, the plant pathogen Agrobacterium tumefaciens, and the animal pathogen Brucella abortus. This review outlines the similarities and differences of the AbcR sRNAs between each of these organisms, and discusses reasons as to why this group of sRNAs has diverged in their genetic organization and regulatory functions across species...
October 27, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29062811/activation-of-nf-kb-mediated-tnf-induced-antimicrobial-immunity-is-required-for-the-efficient-brucella-abortus-clearance-in-raw-264-7-cells
#17
Huynh T Hop, Alisha W B Reyes, Tran X N Huy, Lauren T Arayan, WonGi Min, Hu J Lee, Man H Rhee, Hong H Chang, Suk Kim
In this study, we explore the regulatory roles of pro-inflammatory cytokine tumor necrosis factor alpha (TNF) in the innate immunity of macrophages against B. abortus infection. We show that infection of macrophage with B. abortus induces marked expression and secretion of TNF which subsequently binds to TNF receptor 1 (TNFR-1) and activates a downstream signaling cascade of the innate immunity. Blocking of TNF signaling resulted in a notable increase of B. abortus survival which was associated with an increase of anti-inflammatory cytokine interleukin 10 (IL-10), a beneficial effector of Brucella survival, as well as remarkable decrease of reactive oxygen species (ROS) and nitric oxide (NO), antibrucella molecules...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29055858/deletion-of-the-transcriptional-regulator-gntr-down-regulated-the-expression-of-genes-related-to-virulence-and-conferred-protection-against-wild-type-brucella-challenge-in-balb-c-mice
#18
Zhi-Qiang Li, Jin-Liang Zhang, Li Xi, Guang-Li Yang, Shu-Li Wang, Xiao-Gen Zhang, Jun-Bo Zhang, Hui Zhang
Brucellosis, which is caused by Brucella spp., is a zoonotic infectious disease that can cause great hazard to public health and safety. The virulence of Brucella is essential for survive and multiply in host macrophages. GntR is a transcriptional regulator in Brucella that is required for virulence in macrophages and mice, and involved in resistance to stress responses. To determine the expression levels of target genes of GntR, we detected the expression levels of the GntR target genes in Brucella infected BALB/c mice...
October 19, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29054743/simultaneous-rna-seq-based-transcriptional-profiling-of-intracellular-brucella-abortus-and-b-abortus-infected-murine-macrophages
#19
Huynh Tan Hop, Lauren Togonon Arayan, Alisha Wehdnesday Bernardo Reyes, Tran Xuan Ngoc Huy, WonGi Min, Hu Jang Lee, Jee Soo Son, Suk Kim
Brucella is a zoonotic pathogen that survives within macrophages; however the replicative mechanisms involved are not fully understood. We describe the isolation of sufficient Brucella abortus RNA from primary host cell environment using modified reported methods for RNA-seq analysis, and simultaneously characterize the transcriptional profiles of intracellular B. abortus and bone marrow-derived macrophages (BMM) from BALB/c mice at 24 h (replicative phase) post-infection. Our results revealed that 25.12% (801/3190) and 16...
October 18, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29041788/use-of-i-brucella-abortus-i-species-specific-polymerase-chain-reaction-assay-for-the-diagnosis-of-bovine-brucellosis
#20
Songelwayo L Chisi, Tracy Schmidt, George W Akol, Henriette Van Heerden
Serology is primarily used in the diagnosis of bovine brucellosis. Bacterial culture and isolation is the gold standard in diagnosing brucellosis but, like serology, it does not offer complete (100%) diagnostic sensitivity and specificity. Polymerase chain reaction (PCR) has been suggested to offer better specificity and sensitivity. In this study, we evaluated the performance of Brucella abortus species specific (BaSS) PCR directly from different samples in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture...
September 27, 2017: Journal of the South African Veterinary Association
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