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Brucella abortus

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https://www.readbyqxmd.com/read/28443226/a-novel-pcr-assay-for-detecting-brucella-abortus-and-brucella-melitensis
#1
Saeed Alamian, Majid Esmaelizad, Taghi Zahraei, Afshar Etemadi, Mohsen Mohammadi, Davoud Afshar, Soheila Ghaderi
OBJECTIVES: Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis. METHODS: All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all available Brucella sequences. A unique repeat sequence (URS) locus on chromosome 1 could differentiate Brucella abortus from Brucella melitensis...
February 2017: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/28398652/brucella-abortus-activated-microglia-induce-neuronal-death-through-primary-phagocytosis
#2
Ana M Rodríguez, M Victoria Delpino, M Cruz Miraglia, Miriam M Costa Franco, Paula Barrionuevo, Vida A Dennis, Sergio C Oliveira, Guillermo H Giambartolomei
Inflammation has long been implicated as a contributor to pathogenesis in neurobrucellosis. Many of the associated neurocognitive symptoms of neurobrucellosis may be the result of neuronal dysfunction resulting from the inflammatory response induced by Brucella abortus infection in the central nervous system. In this manuscript, we describe an immune mechanism for inflammatory activation of microglia that leads to neuronal death upon B. abortus infection. B. abortus was unable to infect or harm primary cultures of mouse neurons...
April 11, 2017: Glia
https://www.readbyqxmd.com/read/28391992/protein-protein-dna-dna-and-dna-protein-based-vaccination-strategies-using-truncated-omp2b-against-brucella-infection-in-balb-c-mice
#3
Maryam Golshani, Sima Rafati, Mehdi Nejati-Moheimani, Somaye Pourabdi, Amin Arsang, Saeid Bouzari
The purpose of the present study was to evaluate the immunogenicity and protective efficacy of the truncated form of outer membrane protein 2b (TOmp2b) from Brucella abortus in BALB/c mice. Three immunization regimens Protein/Protein, DNA/DNA and DNA/Protein were used. Immunization of mice with all vaccine strategies elicited a strong specific IgG responses (IgG2a titers over IgG1) and provided T helper1 (Th1) oriented immune responses. Furthermore, Protein/Protein (Pro/Pro-) and DNA/Pro- vaccinated groups conferred protection levels against B...
March 28, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28376905/characterization-of-brucella-abortus-mutant-strain-%C3%AE-22915-a-potential-vaccine-candidate
#4
Yanqing Bao, Mingxing Tian, Peng Li, Jiameng Liu, Chan Ding, Shengqing Yu
Brucellosis, caused by Brucella spp., is an important zoonosis worldwide. Vaccination is an effective strategy for protection against Brucella infection in livestock in developing countries and in wildlife in developed countries. However, current vaccine strains including S19 and RB51 are pathogenic to humans and pregnant animals, limiting their use. In this study, we constructed the Brucella abortus (B. abortus) S2308 mutant strain Δ22915, in which the putative lytic transglycosylase gene BAB_RS22915 was deleted...
April 4, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28296882/detection-and-characterization-of-brucella-spp-in-bovine-milk-in-small-scale-urban-and-peri-urban-farming-in-tajikistan
#5
Elisabeth Lindahl-Rajala, Tove Hoffman, David Fretin, Jacques Godfroid, Nosirjon Sattorov, Sofia Boqvist, Åke Lundkvist, Ulf Magnusson
Brucellosis is one of the most common zoonoses globally, and Central Asia remains a Brucella hotspot. The World Health Organization classifies brucellosis as a neglected zoonotic disease that is rarely in the spotlight for research and mainly affects poor, marginalized people. Urban and peri-urban farming is a common practice in many low-income countries, and it increases the incomes of families that are often restrained by limited economic resources. However, there is a concern that the growing number of people and livestock living close together in these areas will increase the transmission of zoonotic pathogens such as Brucella...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28284850/molecular-prevalence-of-putative-virulence-associated-genes-in-brucella-melitensis-and-brucella-abortus-isolates-from-human-and-livestock-specimens-in-iran
#6
Iman Hashemifar, Abbas Yadegar, Faramarz Masjedian Jazi, Nour Amirmozafari
Molecular prevalence of nine putative virulence factors in two more prevalent Brucella species in Iranian patients and livestock was investigated. During five years (2010-2015), 120 human and animal specimens were collected from three geographical areas of Iran. All samples were cultured in blood culture media and subcultured into Brucella agar medium. Nine primer pairs were designed for detection of VirB2, VirB5, VceC, BtpA, BtpB, PrpA, BetB, BPE275 and BSPB virulence factors using PCR and sequence analysis...
March 8, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28281771/an-evaluation-of-serological-tests-in-the-diagnosis-of-bovine-brucellosis-in-naturally-infected-cattle-in-kwazulu-natal-province-in-south-africa
#7
Songelwayo L Chisi, Yoanda Marageni, Prebashni Naidoo, Gloria Zulu, George W Akol, Henriette Van Heerden
The diagnostic sensitivity (DSe) of the Rose Bengal test (RBT), the complement fixation test (CFT), the serum agglutination test (SAT), the competitive enzyme-linked immunosorbent assay (cELISA) and the indirect ELISA (iELISA) were determined in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture (gold standard). Natural brucellosis infection status of animals was determined by culturing and identification of Brucella abortus biovar 1 from abomasal fluid, milk, hygroma fluid, lymph nodes or uterine discharges samples...
February 28, 2017: Journal of the South African Veterinary Association
https://www.readbyqxmd.com/read/28265268/brucella-and-osteoarticular-cell-activation-partners-in-crime
#8
REVIEW
Guillermo H Giambartolomei, Paula C Arriola Benitez, M Victoria Delpino
Osteoarticular brucellosis is the most common presentation of human active disease although its prevalence varies widely. The three most common forms of osteoarticular involvement are sacroiliitis, spondylitis, and peripheral arthritis. The molecular mechanisms implicated in bone damage have been recently elucidated. B. abortus induces bone damage through diverse mechanisms in which TNF-α and the receptor activator of nuclear factor kappa-B ligand (RANKL)-the natural modulator of bone homeostasis are involved...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28264909/promotion-and-rescue-of-intracellular-brucella-neotomae-replication-during-coinfection-with-legionella-pneumophila
#9
Yoon-Suk Kang, James E Kirby
We established a new Brucella neotomaein vitro model system for study of type IV secretion system-dependent (T4SS) pathogenesis in the Brucella genus. Importantly, B. neotomae is a rodent pathogen, and unlike B. abortus, B. melitensis, and B. suis, B. neotomae has not been observed to infect humans. It therefore can be handled more facilely using biosafety level 2 practices. More particularly, using a series of novel fluorescent protein and lux operon reporter systems to differentially label pathogens and track intracellular replication, we confirmed T4SS-dependent intracellular growth of B...
May 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28262966/serologic-evidence-of-brucella-infection-in-pinnipeds-along-the-coast-of-hokkaido-the-northernmost-main-island-of-japan
#10
Erika Abe, Kazue Ohishi, Tsuyoshi Ishinazaka, Kei Fujii, Tadashi Maruyama
Brucella infection in Hokkaido was serologically surveyed in four species of pinnipeds inhabiting Cape Erimo during 2008-2013 and the Shiretoko Peninsula in 1999 by ELISA using Brucella abortus and B. canis as antigens. Anti-Brucella positive sera showed higher absorbance to B. abortus than B. canis in almost all samples. Anti-B. abortus antibodies were detected in serum samples from 24% (n = 55) of Western Pacific harbor seals (Phoca vitulina stejnegeri) in Cape Erimo and from 66% (n = 41) of spotted seals (P...
April 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28261222/u-omp19-from-brucella-abortus-is-a-useful-adjuvant-for-vaccine-formulations-against-salmonella-infection-in-mice
#11
Gabriela S Risso, Marianela V Carabajal, Laura A Bruno, Andrés E Ibañez, Lorena M Coria, Karina A Pasquevich, Seung-Joo Lee, Stephen J McSorley, Gabriel Briones, Juliana Cassataro
Most pathogens infect through mucosal surfaces, and parenteral immunization typically fails to induce effective immune responses at these sites. Development of oral-administered vaccines capable of inducing mucosal as well as systemic immunity while bypassing the issues of antigen degradation and immune tolerance could be crucial for the control of enteropathogens. This study demonstrates that U-Omp19, a bacterial protease inhibitor with immunostimulatory features, coadministered with Salmonella antigens by the oral route, enhances mucosal and systemic immune responses in mice...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28248684/neonatal-brucellosis-a-case-report
#12
Abdul Rahman M Alnemri, Adnan Hadid, Shaik Asfaq Hussain, Ali M Somily, Badr H Sobaih, Abdulkarim Alrabiaah, Awad Alanazi, Zahid Shakoor, Sarah AlSubaie, Naema Meriki, Abdelmageed M Kambal
Although brucellosis is not uncommon in Saudi Arabia, neonatal brucellosis has been infrequently reported. In this case of neonatal brucellosis, Brucella abortus was isolated by blood culture from both the mother and the neonate. Serology was positive only in the mother.
February 28, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28243986/transcriptional-regulator-gntr-of-brucella-abortus-regulates-cytotoxicity-induces-the-secretion-of-inflammatory-cytokines-and-affects-expression-of-the-type-iv-secretion-system-and-quorum-sensing-system-in-macrophages
#13
Zhiqiang Li, Shuli Wang, Hui Zhang, Jinliang Zhang, Li Xi, Junbo Zhang, Chuangfu Chen
The pathogenic mechanisms of Brucella are still poorly understood. GntR is a transcriptional regulator and plays an important role in the intracellular survival of Brucella. To investigate whether GntR is involved in the cytotoxicity of Brucella abortus (B. abortus), we created a 2308ΔgntR mutant of B. abortus 2308 (S2308). Lactate dehydrogenase (LDH) cytotoxicity assays using a murine macrophage cell line (RAW 264.7) show that high-dose infection with the parental strain produces a high level of cytotoxicity to macrophages, but the 2308ΔgntR mutant exhibits a very low level of cytotoxicity, indicating that mutation of GntR impairs the cytotoxicity of B...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28183751/genome-sequences-of-human-and-livestock-isolates-of-brucella-melitensis-and-brucella-abortus-from-the-country-of-georgia
#14
Ketevan Sidamonidze, Jun Hang, Yu Yang, George Dzavashvili, Ekaterine Zhgenti, Nino Trapaidze, Paata Imnadze, Mikeljon P Nikolich
Brucellosis, which is among the most widespread global zoonotic diseases, is endemic in the nation of Georgia and causes substantial human morbidity and economic loss. Here, we report whole-genome sequences of three Brucella melitensis and seven Brucella abortus isolates from cattle, sheep, and humans that represent genetic groups discovered in Georgia.
February 9, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28167945/tlr7-and-tlr3-sense-brucella-abortus-rna-to-induce-proinflammatory-cytokine-production-but-they-are-dispensable-for-host-control-of-infection
#15
Priscila C Campos, Marco Túlio R Gomes, Erika S Guimarães, Gabriela Guimarães, Sergio C Oliveira
Brucella abortus is a Gram-negative, facultative intracellular bacterium that causes brucellosis, a worldwide zoonotic disease leading to undulant fever in humans and abortion in cattle. The immune response against this bacterium relies on the recognition of microbial pathogen-associated molecular patterns, such as lipoproteins, lipopolysaccharides, and DNA; however, the immunostimulatory potential of B. abortus RNA remains to be elucidated. Here, we show that dendritic cells (DCs) produce significant amounts of IL-12, IL-6, and IP-10/CXCL10, when stimulated with purified B...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28160160/the-unexpected-discovery-of-brucella-abortus-buck-19-vaccine-in-goats-from-ecuador-underlines-the-importance-of-biosecurity-measures
#16
Jorge Ron-Román, Dirk Berkvens, Daniela Barzallo-Rivadeneira, Alexandra Angulo-Cruz, Pablo González-Andrade, Elizabeth Minda-Aluisa, Washington Benítez-Ortíz, Jef Brandt, Richar Rodríguez-Hidalgo, Claude Saegerman
Very few, mostly old, and only preliminary serological studies of brucellosis in goats exist in Ecuador. In order to assess the current epidemiological situation, we performed a cross-sectional serological study in the goat populations of Carchi (n = 160 animals), Pichincha (n = 224 animals), and Loja provinces (n = 2024 animals). Only two positive serological results (RB negative and SAT-EDTA ≥400 IU/ml) were obtained in lactating goats from the same farm in Quito (Pichincha province). Additionally, milk was sampled from 220 animals in Pichincha province...
March 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28152976/origins-and-global-context-of-brucella-abortus-in-italy
#17
Giuliano Garofolo, Elisabetta Di Giannatale, Ilenia Platone, Katiuscia Zilli, Lorena Sacchini, Anna Abass, Massimo Ancora, Cesare Cammà, Guido Di Donato, Fabrizio De Massis, Paolo Calistri, Kevin P Drees, Jeffrey T Foster
BACKGROUND: Brucellosis is a common and chronic disease of cattle and other bovids that often causes reproductive disorders. Natural infection in cattle is caused by Brucella abortus and transmission typically occurs during abortions, calving, or nursing. Brucellosis is also a major zoonotic disease due to contamination of dairy products or contact with the tissues of infected animals. Brucellosis has been eradicated from most of the developed world in the last 40 years but persists in many regions-the disease remains prevalent in portions of Africa, the Middle East, Asia, and Central and South America, as well as in the Mediterranean basin...
February 2, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28131952/brucella-abortus-2308%C3%AE-nodv%C3%AE-nodw-double-mutant-is-highly-attenuated-and-confers-protection-against-wild-type-challenge-in-balb-c-mice
#18
Zhiqiang Li, Shuli Wang, Jinliang Zhang, Guangli Yang, Baodong Yuan, Jie Huang, Jincheng Han, Li Xi, Yanren Xiao, Chuangfu Chen, Hui Zhang
Brucellosis is an important zoonotic disease of worldwide distribution, which causes animal and human disease. However, the current Brucella abortus (B. abortus) vaccines (S19 and RB51) have several drawbacks, including residual virulence for animals and humans. Moreover, S19 cannot allow serological differentiation between infected and vaccinated animals. We constructed double deletion (ΔNodVΔNodW) mutant from virulent B. abortus 2308 (S2308) by deleting the genes encoding two-component regulatory system (TCS) in chromosome II in S2308...
January 25, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28129787/seroprevalence-and-risk-factors-associated-with-bovine-brucellosis-in-the-potohar-plateau-pakistan
#19
Shahzad Ali, Shamim Akhter, Heinrich Neubauer, Falk Melzer, Iahtasham Khan, Emmanuel Nji Abatih, Hosny El-Adawy, Muhammad Irfan, Ali Muhammad, Muhammad Waqas Akbar, Sajid Umar, Qurban Ali, Muhammad Naeem Iqbal, Abid Mahmood, Haroon Ahmed
BACKGROUND: The seroprevalence and risk factors of bovine brucellosis were studied at animal and herd level using a combination of culture, serological and molecular methods. The study was conducted in 253 randomly selected cattle herds of the Potohar plateau, Pakistan from which a total of 2709 serum (1462 cattle and 1247 buffaloes) and 2330 milk (1168 cattle and 1162 buffaloes) samples were collected. Data on risk factors associated with seroprevalence of brucellosis were collected through interviews using questionnaires...
January 28, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28126739/structure-based-mutational-studies-of-o-acetylserine-sulfhydrylase-reveal-the-reason-for-the-loss-of-cysteine-synthase-complex-formation-in-brucella-abortus
#20
Sudhaker Dharavath, Isha Raj, Samudrala Gourinath
Cysteine biosynthesis takes place via a two-step pathway in bacteria, fungi, plants and protozoan parasites, but not in humans, and hence the machinery of cysteine biosynthesis is an opportune target for therapeutics. The decameric cysteine synthase complex (CSC) is formed when the C-terminal tail of serine acetyl transferase (SAT) binds in the active site of O-acetyl serine sulfydrylase (OASS), playing a role in the regulation of this pathway. Here we show that OASS from Brucella abortus (BaOASS) does not interact with its cognate SAT C-terminal tail...
January 26, 2017: Biochemical Journal
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