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Listeria monocytogenes

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https://www.readbyqxmd.com/read/29166172/efficacy-of-listex-p100-at-different-concentrations-for-reduction-of-listeria-monocytogenes-inoculated-in-sashimi
#1
S Miguéis, C Saraiva, A Esteves
Sushi restaurants have become quite popular in Europe, with an increase in the consumption of the sashimi speciality. Pathogenic bacteria such as Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus have been reported in this kind of food. Controlling the presence and multiplication of L. monocytogenes is a challenge for food safety management systems owing to its ubiquitous presence and psychrotrophic growth. Bacteriophages have been used as pathogenic biocide agents for decades. The bacteriophage P100, present in LISTEX P100, was used in this study to understand the possibility of implementing a new critical control point for L...
November 22, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/29165857/isolation-of-phage-display-library-derived-scfv-antibody-specific-to-listeria-monocytogenes-by-a-novel-immobilized-method
#2
Nguyen Xuan-Hung, Thu-Le Trinh, Huong Thi-Bich Vu, Huan Quang Le, Anh To Kim
AIMS: To select Listeria monocytogenes specific scFv antibodies from a phage display library by a novel simple and cost-effective immobilization method. METHODS AND RESULTS: Light Expanded Clay Aggregate (LECA) was used as biomass support matrix for biopanning of a phage display library to select Listeria monocytogenes specific scFv antibody. Four rounds of positive selection against LECA-immobilized L. monocytogenes and an additional subtractive panning against L...
November 22, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29165457/the-chemical-composition-nutritional-value-and-antimicrobial-properties-of-abelmoschus-esculentus-seeds
#3
Spyridon Petropoulos, Ângela Fernandes, Lillian Barros, Ana Ciric, Marina Sokovic, Isabel C F R Ferreira
Okra is a vegetable crop usually used for its immature pods. The harvest stage (fruit size) depends on consumers' preferences and the fruit that does not meet market requirements is being disposed of. Considering the short time interval from the setting of the fruit to the harvest stage, the present study evaluates the nutritional value, chemical composition, and antioxidant and antimicrobial properties of okra seeds from genotypes cultivated under Mediterranean conditions, as an alternative end-use product...
November 22, 2017: Food & Function
https://www.readbyqxmd.com/read/29163512/carboxyl-terminal-residues-n478-and-v479-required-for-the-cytolytic-activity-of-listeriolysin-o-play-a-critical-role-in-listeria-monocytogenes-pathogenicity
#4
Changyong Cheng, Li Jiang, Tiantian Ma, Hang Wang, Xiao Han, Jing Sun, Yongchun Yang, Zhongwei Chen, Huifei Yu, Yi Hang, Fengdan Liu, Bosen Wang, Weihuan Fang, Huarong Huang, Chun Fang, Chang Cai, Nancy Freitag, Houhui Song
Listeria monocytogenes is a facultative intracellular pathogen that secretes the cytolysin listeriolysin O (LLO), which enables the bacteria to cross the phagosomal membrane. L. monocytogenes regulates LLO activity in the phagosome and minimizes its activity in the host cytosol. Mutants that fail to compartmentalize LLO activity are cytotoxic and have attenuated virulence. Here, we showed that residues N478 and V479 of LLO are required for LLO hemolytic activity and bacterial virulence. A single N478A mutation (LLON478A) significantly increased the hemolytic activity of LLO at a neutral pH, while no difference was observed at the optimum acidic pH, compared with wild-type LLO...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29163410/modeling-reveals-the-role-of-aging-and-glucose-uptake-impairment-in-l1a1-listeria-monocytogenes-biofilm-life-cycle
#5
Eva Balsa-Canto, Carlos Vilas, Alejandro López-Núñez, Maruxa Mosquera-Fernández, Romain Briandet, Marta L Cabo, Carlos Vázquez
Listeria monocytogenes is a food-borne pathogen that can persist in food processing plants by forming biofilms on abiotic surfaces. The benefits that bacteria can gain from living in a biofilm, i.e., protection from environmental factors and tolerance to biocides, have been linked to the biofilm structure. Different L. monocytogenes strains build biofilms with diverse structures, and the underlying mechanisms for that diversity are not yet fully known. This work combines quantitative image analysis, cell counts, nutrient uptake data and mathematical modeling to provide a mechanistic insight into the dynamics of the structure of biofilms formed by L...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29155896/progesterone-impairs-antigen-non-specific-immune-protection-by-cd8-t-memory-cells-via-interferon-%C3%AE-gene-hypermethylation
#6
Yushi Yao, Hui Li, Jie Ding, Yixin Xia, Lei Wang
Pregnant women and animals have increased susceptibility to a variety of intracellular pathogens including Listeria monocytogenes (LM), which has been associated with significantly increased level of sex hormones such as progesterone. CD8 T memory(Tm) cell-mediated antigen-non-specific IFN-γ responses are critically required in the host defense against LM. However, whether and how increased progesterone during pregnancy modulates CD8 Tm cell-mediated antigen-non-specific IFN-γ production and immune protection against LM remain poorly understood...
November 20, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29155570/chemistry-antimicrobial-mechanisms-and-antibiotic-activities-of-cinnamaldehyde-against-pathogenic-bacteria-in-animal-feeds-and-human-foods
#7
Mendel Friedman
Cinnamaldehyde is a major constituent of cinnamon essential oils produced by aromatic cinnamon plants. This compound has been reported to exhibit antimicrobial properties in vitro in laboratory media and in animal feeds and human foods contaminated with disease-causing bacteria including Bacillus cereus, Campylobacter jejuni, Clostridium perfringens, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. This integrated review surveys and interprets our current knowledge of the chemistry, analysis, safety, mechanism of action, and antibiotic activities of cinnamaldehyde in food animal (cattle, lambs, calves, pigs, poultry) diets and in widely consumed liquid (apple, carrot, tomato, and watermelon juices, milk) and solid foods...
November 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29150567/amplifying-ifn-%C3%AE-signaling-in-dendritic-cells-by-cd11c-specific-loss-of-socs1-increases-innate-immunity-to-infection-while-decreasing-adaptive-immunity
#8
Alejandro F Alice, Gwen Kramer, Shelly Bambina, Jason R Baird, Keith S Bahjat, Michael J Gough, Marka R Crittenden
Although prophylactic vaccines provide protective humoral immunity against infectious agents, vaccines that elicit potent CD8 T cell responses are valuable tools to shape and drive cellular immunity against cancer and intracellular infection. In particular, IFN-γ-polarized cytotoxic CD8 T cell immunity is considered optimal for protective immunity against intracellular Ags. Suppressor of cytokine signaling (SOCS)1 is a cross-functional negative regulator of TLR and cytokine receptor signaling via degradation of the receptor-signaling complex...
November 17, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29150238/a-t-cell-receptor-locus-harbors-a-malaria-specific-immune-response-gene
#9
Natalija Van Braeckel-Budimir, Stephanie Gras, Kristin Ladell, Tracy M Josephs, Lecia Pewe, Stina L Urban, Kelly L Miners, Carine Farenc, David A Price, Jamie Rossjohn, John T Harty
Immune response (Ir) genes, originally proposed by Baruj Benacerraf to explain differential antigen-specific responses in animal models, have become synonymous with the major histocompatibility complex (MHC). We discovered a non-MHC-linked Ir gene in a T cell receptor (TCR) locus that was required for CD8(+) T cell responses to the Plasmodium berghei GAP5040-48 epitope in mice expressing the MHC class I allele H-2D(b). GAP5040-48-specific CD8(+) T cell responses emerged from a very large pool of naive Vβ8...
November 21, 2017: Immunity
https://www.readbyqxmd.com/read/29147103/antibacterial-effects-against-various-foodborne-pathogens-and-sensory-properties-of-yogurt-supplemented-with-panax-ginseng-marc-extract
#10
Su Jin Eom, Ji Eun Hwang, Kee-Tae Kim, Hyun-Dong Paik
Panax ginseng marc is produced from fresh ginseng roots during processing and is generally treated as industrial waste. The primary aim of this study was to improve its utilization in the dairy industry as a potential high-value resource. Yogurt was prepared from 11% skim milk powder, 0.1% pectin, 10% sucrose, and ginseng marc ethanol extract (GME, 0.5% and 1.0%) in milk, and was inoculated with a 0.02% yogurt culture (Lactobacillus acidophilus, Bifidobacterium longum, and Streptococcus thermophilus). After fermentation at 40°C for 6-8 h, the physicochemical properties of samples were analyzed by the AOAC, Kjeldahl, and Soxhlet methods...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29145803/unraveling-the-evolution-and-coevolution-of-small-regulatory-rnas-and-coding-genes-in-listeria
#11
Franck Cerutti, Ludovic Mallet, Anaïs Painset, Claire Hoede, Annick Moisan, Christophe Bécavin, Mélodie Duval, Olivier Dussurget, Pascale Cossart, Christine Gaspin, Hélène Chiapello
BACKGROUND: Small regulatory RNAs (sRNAs) are widely found in bacteria and play key roles in many important physiological and adaptation processes. Studying their evolution and screening for events of coevolution with other genomic features is a powerful way to better understand their origin and assess a common functional or adaptive relationship between them. However, evolution and coevolution of sRNAs with coding genes have been sparsely investigated in bacterial pathogens. RESULTS: We designed a robust and generic phylogenomics approach that detects correlated evolution between sRNAs and protein-coding genes using their observed and inferred patterns of presence-absence in a set of annotated genomes...
November 16, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29144454/dynamics-of-p-type-atpase-transport-revealed-by-single-molecule-fret
#12
Mateusz Dyla, Daniel S Terry, Magnus Kjaergaard, Thomas L-M Sørensen, Jacob Lauwring Andersen, Jens Peter Andersen, Charlotte Rohde Knudsen, Roger B Altman, Poul Nissen, Scott C Blanchard
Phosphorylation-type (P-type) ATPases are ubiquitous primary transporters that pump cations across cell membranes through the formation and breakdown of a phosphoenzyme intermediate. Structural investigations suggest that the transport mechanism is defined by conformational changes in the cytoplasmic domains of the protein that are allosterically coupled to transmembrane helices so as to expose ion binding sites to alternate sides of the membrane. Here, we have used single-molecule fluorescence resonance energy transfer to directly observe conformational changes associated with the functional transitions in the Listeria monocytogenes Ca(2+)-ATPase (LMCA1), an orthologue of eukaryotic Ca(2+)-ATPases...
November 16, 2017: Nature
https://www.readbyqxmd.com/read/29140746/inactivation-kinetics-of-pathogens-during-thermal-processing-in-acidified-broth-and-tomato-pur%C3%A3-e-ph-4-5
#13
Evann L Dufort, Jonathan Sogin, Mark R Etzel, Barbara H Ingham
Thermal inactivation kinetics for single strains of Shiga toxin-producing Escherichia coli (STEC), Listeria monocytogenes, and Salmonella enterica were measured in acidified tryptic soy broth (TSB; pH 4.5) heated at 54°C. Inactivation curves also were measured for single-pathogen five-strain cocktails of E. coli O157:H7, L. monocytogenes, and S. enterica heated in tomato purée (pH 4.5) at 52, 54, 56, and 58°C. Inactivation curves were fit using log-linear and nonlinear (Weibull) models. The Weibull model yields the time for a 5-log reduction (t*) and a curve shape parameter (β)...
November 15, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/29134590/application-of-a-virucidal-agent-to-avoid-overestimation-of-phage-kill-during-phage-decontamination-assays-on-ready-to-eat-meats
#14
Andrew Chibeu, S Balamurugan
We describe a method for assessing the effectiveness of tea extract based virucide (TeaF) application to remove phage LISTEX™ P100 not bound to Listeria monocytogenes from stomached rinses prior to direct plating and bacterial enumeration, where the phage is being used as a decontaminant to reduce L. monocytogenes levels on ready-to-eat meat.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29133393/sequential-induction-of-fur-regulated-genes-in-response-to-iron-limitation-in-bacillus-subtilis
#15
Hualiang Pi, John D Helmann
Bacterial cells modulate transcription in response to changes in iron availability. The ferric uptake regulator (Fur) senses intracellular iron availability and plays a central role in maintaining iron homeostasis in Bacillus subtilis Here we utilized FrvA, a high-affinity Fe(2+) efflux transporter from Listeria monocytogenes, as an inducible genetic tool to deplete intracellular iron. We then characterized the responses of the Fur, FsrA, and PerR regulons as cells transition from iron sufficiency to deficiency...
November 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29132042/long-range-interactions-keep-bacterial-cells-from-liquid-solid-interfaces-evidence-of-a-bacteria-exclusion-zone-near-nafion-surfaces-and-possible-implications-for-bacterial-attachment
#16
Yifan Cheng, Carmen I Moraru
Hydrophilic surfaces of both abiotic and biological origin have been shown to bear particle-exclusion zones as large as hundreds of micrometers at liquid-solid interfaces. Here we present the first systematic investigation and evidence for bacteria-free exclusion zones for several bacterial strains, including pathogens associated with hospital infections and/or foodborne outbreaks: Staphylococcus aureus, Escherichia coli O157:H7, and Listeria monocytogenes. Tests were carried out both in a phosphate buffer, as well as triptic soy broth (TSB) of high ionic strength...
November 10, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29128397/identification-and-characterization-of-molluscan-caveolin-1-ortholog-from-haliotis-discus-discus-possible-involvement-in-embryogenesis-and-host-defense-mechanism-against-pathogenic-stress
#17
H M V Udayantha, S D N K Bathige, Thanthrige Thiunuwan Priyathilaka, Sukkyoung Lee, Myoung-Jin Kim, Jehee Lee
Caveolins are principal membrane proteins of caveolae that play a central role in signal transduction, substrate transport, and membrane trafficking in various cell types. Numerous studies have reported the crucial role of caveolin-1 (CAV1) in response to invading microbes; yet, very little is known about molluscan CAV1. In this study, we identified and characterized CAV1 ortholog from the disk abalone, Haliotis discus discus (HdCAV1). The cDNA sequence of HdCAV1 is 826 bp long and encodes a 127-amino acid polypeptide...
November 8, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29127810/antimicrobial-activities-of-gaseous-essential-oils-against-listeria-monocytogenes-on-a-laboratory-medium-and-radish-sprouts
#18
Gyeongmin Lee, Yoonbin Kim, Hoikyung Kim, Larry R Beuchat, Jee-Hoon Ryu
The aim of this study was to evaluate the antimicrobial activities of gaseous essential oils (EO gases) against Listeria monocytogenes on the surfaces of a laboratory medium and radish sprouts. We determined the minimal inhibitory concentration (MIC) and minimal lethal concentration (MLC) values of EO gases from eight EOs extracted from basil leaves, carrot seed, cinnamon bark, cinnamon leaves, clove flower buds, oregano leaves, thyme flowers (linalool), and thyme leaves (thymol) against L. monocytogenes on a nutrient agar supplemented with 1% glucose and 0...
November 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29124040/cold-shock-domain-family-proteins-csps-are-involved-in-regulation-of-virulence-cellular-aggregation-and-flagella-based-motility-in-listeria-monocytogenes
#19
Athmanya K Eshwar, Claudia Guldimann, Anna Oevermann, Taurai Tasara
Cold shock-domain family proteins (Csps) are highly conserved nucleic acid binding proteins regulating the expression of various genes including those involved in stress resistance and virulence in bacteria. We show here that Csps are involved in virulence, cell aggregation and flagella-based extracellular motility of Listeria monocytogenes. A L. monocytogenes mutant deleted in all three csp genes (ΔcspABD) is attenuated with respect to human macrophage infection as well as virulence in a zebrafish infection model...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29121703/effect-of-acta-gene-on-the-invasion-efficiency-of-listeria-monocytogenes-as-observed-in-healthy-and-senescent-intestinal-epithelial-cells
#20
Jimyeong Ha, Hyemin Oh, Sejeong Kim, Jeeyeon Lee, Soomin Lee, Heeyoung Lee, Yukyung Choi, Sung Sil Moon, Kyoung-Hee Choi, Yohan Yoon
Listeria monocytogenes can asymptomatically inhabit the human intestine as a commensal bacterium. However, the mechanism by which L. monocytogenes is able to inhabit the intestine without pathogenic symptoms remains unclear. Here, we compared the invasion efficiency of L. monocytogenes strains with the 268- and 385-bp-long actA gene. Clinical strains SMFM-CI-3 and SMFM-CI-6 with 268-bp actA isolated from patients with listeriosis and strains SMFM-SI-1 and SMFM-SI-2 with the 385-bp gene isolated from carcasses were used for inoculum preparation...
November 9, 2017: Journal of Microbiology and Biotechnology
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