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Listeria monocytogenes

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https://www.readbyqxmd.com/read/28713788/microorganisms-of-the-intestinal-microbiota-of-oncorhynchus-mykiss-produce-antagonistic-substances-against-bacteria-contaminating-food-and-causing-disease-in-humans
#1
Olga Lobos, Andrea Barrera, Carlos Padilla
The objective was to analyse the antibacterial ability of members of the intestinal microbiota of Oncorhynchus mykiss specimens on several bacterial target strains that contaminate food and cause disease in humans. Bacterial colonies from an intestinal portion of the 20 specimens of O. mykiss were obtained in different culture media. Several of the colonies showed antibacterial action on different target strains. The bacterial species with the highest antagonistic capacity were Hafnia alvei and Lactococcus lactis and the more susceptible target strains were Aeromonas hydrophila, Listeria monocytogenes and Escherichia coli...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713785/inactivation-of-heat-adapted-and-chlorine-adapted-listeria-monocytogenes-atcc-7644-on-tomatoes-using-sodium-dodecyl-sulphate-levulinic-acid-and-sodium-hypochlorite-solution
#2
Oluwatosin Ademola Ijabadeniyi, Elizabeth Mnyandu
The effectiveness of sodium dodecyl sulphate (SDS), sodium hypochlorite solution and levulinic acid in reducing the survival of heat adapted and chlorine adapted Listeria monocytogenes ATCC 7644 was evaluated. The results against heat adapted L. monocytognes revealed that sodium hypochlorite solution was the least effective, achieving log reduction of 2.75, 2.94 and 3.97 log colony forming unit (CFU)/mL for 1, 3 and 5 minutes, respectively. SDS was able to achieve 8 log reduction for both heat adapted and chlorine adapted bacteria...
April 13, 2017: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28713514/detection-of-eight-foodborne-bacterial-pathogens-by-oligonucleotide-array-hybridization
#3
Zohreh Nasrabadi, Reza Ranjbar, Fatemeh Poorali, Meysam Sarshar
BACKGROUND: Simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses. OBJECTIVE: The aim of this study was to evaluate the use of 16S rDNA and 23S rDNA sequences as targets for simultaneous detection of eight foodborne bacterial pathogens. METHODS: Nineteen bacterial oligonucleotide probes were synthesized and applied to nylon membranes. Digoxygenin labeled 16S rDNA and 23S rDNA from bacteria were amplified by PCR using universal primers, and the amplicons were hybridized to the membrane array...
May 2017: Electronic Physician
https://www.readbyqxmd.com/read/28713103/-listeriosis-of-central-nervous-system-in-patients-with-ulcerative-colitis-case-study
#4
Agnieszka Żak-Gołąb, Krzysztof Dąbrowski, Antoni Hrycek
In the recently an increase morbidity inflammatory bowel disease, including ulcerative colitis was observed. The use of purine analogs and their metabolites are associated with a higher incidence of infections in this group of patients. Listeriosis is an infectious disease caused by a relatively anaerobic gram-positive bacteria - Listeria monocytogenes. Common symptoms include fever, nausea, vomiting and diarrhea, but these pathogens can also cause myocarditis, central nervous system infections, including brain abscesses and sepsis...
2017: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/28711509/listeria-monocytogenes-pdee-a-phosphodiesterase-that-contributes-to-virulence-and-has-hydrolytic-activity-against-cyclic-mononucleotides-and-cyclic-dinucleotides
#5
Swetha Reddy, Gokul Turaga, Hossam Abdelhamed, Michelle M Banes, Robert W Wills, Mark L Lawrence
We have identified and partially characterized a putative HD domain hydrolase, LMOf2365_2464, which is highly expressed during listerial intracellular replication. LMOf2365_2464 is annotated as a putative HD domain-containing hydrolase. The ability of an isogenic mutant strain, F2365Δ2464, to adhere, invade and replicate in intestinal epithelial cells (Caco-2) was significantly lower than parent strain F2365. Colonization of mouse liver and spleen by L. monocytogenes F2365 was significantly higher than it was for the mutant...
July 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28711269/development-of-an-isothermal-amplification-based-assay-for-the-rapid-visual-detection-of-salmonella-bacteria
#6
Hai-Bin Liu, Yu-Xuan Zang, Xin-Jun Du, Ping Li, Shuo Wang
The efficient and timely detection of pathogens is a major concern worldwide. The aim of this study was to establish a rapid detection method for Salmonella bacteria in food samples to facilitate timely treatment. Widely used detection methods currently include culture-based methods and PCR-based methods. The former are time consuming, requiring 2 to 3 d, whereas the latter have higher accuracy but are typically complicated, requiring expertise and expensive instruments. In this study, a sensitive and rapid approach for the visual and point-of-use detection of Salmonella bacteria based on recombinase polymerase amplification (RPA) and a lateral-flow (LF) nucleic acid strip was established...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28710947/variations-in-biofilm-formation-desiccation-resistance-and-benzalkonium-chloride-susceptibility-among-listeria-monocytogenes-strains-isolated-in-canada
#7
Marta J Piercey, Timothy C Ells, Andrew J Macintosh, Lisbeth Truelstrup Hansen
Listeria monocytogenes is a pathogenic foodborne microorganism noted for its ability to survive in the environment and food processing facilities. Survival may be related to the phenotype of individual strains including the ability to form biofilms and resist desiccation and/or sanitizer exposure. The objectives of this research were to compare 14 L. monocytogenes strains isolated from blood (3), food (6) and water (5) with respect to their benzalkonium chloride (BAC) sensitivity, desiccation resistance, and ability to form biofilm...
July 8, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28708050/hyperinvasiveness-and-increased-intercellular-spread-of-listeria-monocytogenes-sequence-type-1-are-independent-of-listeriolysin-s-internalin-f-and-internalin-j1
#8
Sebastian Rupp, Michelle Bärtschi, Joachim Frey, Anna Oevermann
PURPOSE: Listeria monocytogenes is a genetically heterogeneous species, which is divided into evolutionary lineages and clonal complexes (CCs). Not all L. monocytogenes isolates are equally likely to cause disease, with CC1, and in particular sequence type (ST) 1, being the most prevalent complex in human and ruminant infections and more specifically in neurolisteriosis. While the major factors that determine neurotropism are unknown, the L. monocytogenes CC1 strains harbour listeriolysin S (lls) and particular alleles of internalin (inl) F and inlJ, which are not present in CCs commonly isolated from food and the environment...
July 15, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28708030/growth-kinetics-of-listeria-monocytogenes-in-cut-produce
#9
Joelle K Salazar, Surasri N Sahu, Ian M Hildebrandt, Lijie Zhang, Yan Qi, Girvin Liggans, Atin R Datta, Mary Lou Tortorello
Cut produce continues to constitute a significant portion of the fresh fruit and vegetables sold directly to consumers. As such, the safety of these items during storage, handling, and display remains a concern. Cut tomatoes, cut leafy greens, and cut melons, which have been studied in relation to their ability to support pathogen growth, have been specifically identified as needing temperature control for safety. Data are needed on the growth behavior of foodborne pathogens in other types of cut produce items that are commonly offered for retail purchase and are potentially held without temperature control...
July 14, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28706878/a-genetically-modified-attenuated-listeria-vaccine-expressing-hpv16-e7-kill-tumor-cells-in-direct-and-antigen-specific-manner
#10
Yan Yan Jia, Wei Jun Tan, Fei Fei Duan, Zhi Ming Pan, Xiang Chen, Yue Lan Yin, Xin An Jiao
Attenuated Listeria monocytogenes (L. monocytogenes, LM) induces specific CD8(+) and CD4(+) T cell responses, and has been identified as a promising cancer vaccine vector. Cervical cancer is the third most common cancer in women worldwide, with human papillomavirus (HPV), particularly type 16, being the main etiological factor. The therapeutic HPV vaccines are urgently needed. The E7 protein of HPV is necessary for maintaining malignancy in tumor cells. Here, a genetically modified attenuated LM expressing HPV16 E7 protein was constructed...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28705500/development-and-characterization-of-bioactive-edible-films-from-spider-crab-maja-crispata-chitosan-incorporated-with-spirulina-extract
#11
Rafik Balti, Mohamed Ben Mansour, Nadhem Sayari, Lamia Yacoubi, Lotfi Rabaoui, Nicolas Brodu, Anthony Massé
Active food packaging films based on crab chitosan and Spirulina extract (SE) were developed. The effects of the SE incorporation at different levels on physical (color, opacity water vapor and oxygen permeability) and mechanical (tensile strength and elongation at break) properties of chitosan films were investigated. FTIR was carried out to observe the potential modifications of the chitosan films when incorporated with SE. The obtained results suggested that incorporation of SE into chitosan films improved mechanical and barrier properties...
July 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28704812/sglt1-deficiency-turns-listeria-infection-into-a-lethal-disease-in-mice
#12
Piyush Sharma, Vishal Khairnar, Ivana Vrhovac Madunić, Yogesh Singh, Aleksandra Pandyra, Madhuri S Salker, Hermann Koepsell, Ivan Sabolić, Florian Lang, Philipp A Lang, Karl S Lang
BACKGROUND: Cellular glucose uptake may involve either non-concentrative glucose carriers of the GLUT family or Na+-coupled glucose-carrier SGLT1, which accumulates glucose against glucose gradients and may thus accomplish cellular glucose uptake even at dramatically decreased extracellular glucose concentrations. SGLT1 is not only expressed in epithelia but as well in tumour cells and immune cells. Immune cell functions strongly depend on their metabolism, therefore we hypothesized that deficiency of SGLT1 modulates the defence against bacterial infection...
July 14, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28702378/thioredoxin-a-is-essential-for-motility-and-contributes-to-host-infection-of-listeria-monocytogenes-via-redox-interactions
#13
Changyong Cheng, Zhimei Dong, Xiao Han, Hang Wang, Li Jiang, Jing Sun, Yongchun Yang, Tiantian Ma, Chunyan Shao, Xiaodu Wang, Zhongwei Chen, Weihuan Fang, Nancy E Freitag, Huarong Huang, Houhui Song
Microbes employ the thioredoxin system to defend against oxidative stress and ensure correct disulfide bonding to maintain protein function. Listeria monocytogenes has been shown to encode a putative thioredoxin, TrxA, but its biological roles and underlying mechanisms remain unknown. Here, we showed that expression of L. monocytogenes TrxA is significantly induced in bacteria treated with the thiol-specific oxidizing agent, diamide. Deletion of trxA markedly compromised tolerance of the pathogen to diamide, and mainly impaired early stages of infection in human intestinal epithelial Caco-2 cells...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28702010/comparison-among-the-quantification-of-bacterial-pathogens-by-qpcr-dpcr-and-cultural-methods
#14
Matteo Ricchi, Cristina Bertasio, Maria B Boniotti, Nadia Vicari, Simone Russo, Michela Tilola, Marco A Bellotti, Barbara Bertasi
The demand for rapid methods for the quantification of pathogens is increasing. Among these methods, those based on nucleic acids amplification (quantitative PCRs) are the most widespread worldwide. Together with the qPCR, a new approach named digital PCR (dPCR), has rapidly gained importance. The aim of our study was to compare the results obtained using two different dPCR systems and one qPCR in the quantification of three different bacterial pathogens: Listeria monocytogenes, Francisella tularensis, and Mycobacterium avium subsp...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28696370/strategies-for-pathogen-biocontrol-using-lactic-acid-bacteria-and-their-metabolites-a-focus-on-meat-ecosystems-and-industrial-environments
#15
REVIEW
Patricia Castellano, Mariana Pérez Ibarreche, Mariana Blanco Massani, Cecilia Fontana, Graciela M Vignolo
The globalization of trade and lifestyle ensure that the factors responsible for the emergence of diseases are more present than ever. Despite biotechnology advancements, meat-based foods are still under scrutiny because of the presence of pathogens, which causes a loss of consumer confidence and consequently a fall in demand. In this context, Lactic Acid Bacteria (LAB) as GRAS organisms offer an alternative for developing pathogen-free foods, particularly avoiding Listeria monocytogenes, with minimal processing and fewer additives while maintaining the foods' sensorial characteristics...
July 11, 2017: Microorganisms
https://www.readbyqxmd.com/read/28692184/traditional-empiric-antibiotic-treatment-is-still-effective-for-neonatal-fever
#16
Maytal Kristal, Vika Dor, Daniel Glikman
Fever may be the only sign of a serious bacterial infection (SBI) in neonates. Ampicillin plus cefotaxime (A+C) and ampicillin plus gentamicin (A+G) are commonly used as empiric therapies for Escherichia coli, Group B Streptococcus (GBS) and Listeria monocytogenes, which are the most likely neonatal SBI pathogens (1). Culture-based screening and prophylaxis has significantly lowered GBS infections and an alarming increase in the antimicrobial resistance rates of Gram-negative bacteria, including cefotaxime and gentamicin resistance, has been reported (1-3)...
July 10, 2017: Acta Paediatrica
https://www.readbyqxmd.com/read/28691884/synergistic-antimicrobial-combinations-inhibit-and-inactivate-listeria-monocytogenes-in-neutral-and-acidic-broth-systems
#17
Sarah M Kozak, Kyle M Margison, Dennis J D'amico
The use of antimicrobial compounds can be an effective approach to control Listeria monocytogenes in ready-to-eat foods, but it can also be limited by cost, restrictions on concentrations in foods, and potential changes to organoleptic properties. Combinatorial approaches that produce additive or synergistic effects allow for reductions in individual antimicrobial concentrations while achieving the same level of control. The present study determined the MIC and MBC of an antimicrobial compound when used alone or in binary combinations against L...
July 10, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28690383/molecular-docking-based-screening-of-listeriolysin-o-for-improved-inhibitors
#18
Sara Ghafari, Matin Komeilian, Mohaddese Sadat Hashemi, Sareh Oushani, Garshasb Rigi, Behnam Rashidieh, Kamran Yarahmadi, Fatemeh Khoddam
Listeriolysine-O (LLO) is a 50KDa protein responsible for Listeria monocytogenes pathogenicity. The structure of LLO (PDB ID: 4CDB) with domains D1 to D4 is known. Therefore, it is of interest to identify conserved regions among LLO variants for destabilizing oligomerization (50 mer complex) of its monomers using appropriate inhibitors. Therefore, it is of interest to identify suitable inhibitors for inhibiting LLO. Previous reports suggest the use of flavanoids like compounds for inhibiting LLO. Our interest is to identify improved compounds to destabilize LLO oligomerization...
2017: Bioinformation
https://www.readbyqxmd.com/read/28689324/fast-detection-of-listeria-monocytogenes-through-a-nanohybrid-quantum-dot-complex
#19
Wendy Donoso, Ricardo I Castro, Luis Guzmán, Zoraya López-Cabaña, Fabiane M Nachtigall, Leonardo S Santos
Listeria monocytogenes is a recognized foodborne pathogen that causes listeriosis in susceptible consumers. Currently, the detection systems for Listeria in food detect live and dead bacteria, being the viable microorganisms most relevant for their ability to cause sickness in the population at risk. For this reason, a new nanohybrid compound was developed for the optical detection of Listeria that was based on polyamidoamine dendrimers functionalized with an auxotrophic cofactor (lipoic acid), together with the coupling of fluorescent semiconductor crystals (quantum dots)...
July 8, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28687334/a-cysteine-protease-cathepsin-z-from-disk-abalone-haliotis-discus-discus-genomic-characterization-and-transcriptional-profiling-during-bacterial-infections
#20
G I Godahewa, N C N Perera, Sukkyoung Lee, Myoung-Jin Kim, Jehee Lee
Cathepsin Z (CTSZ) is lysosomal cysteine protease of the papain superfamily. It participates in the host immune defense via phagocytosis, signal transduction, cell-cell communication, proliferation, and migration of immune cells such as monocytes, macrophages, and dendritic cells. Hence, CTSZ is also acknowledged as an acute-phase protein in host immunity. In this study, we sought to identify the CTSZ homolog from disk abalone (AbCTSZ) and characterize it at the molecular, genomic, and transcriptional levels...
July 4, 2017: Gene
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