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https://www.readbyqxmd.com/read/28443837/real-time-plasmonic-qpcr-how-fast-is-ultra-fast-30-cycles-in-54-seconds
#1
Philip J R Roche, Mohamed Najih, Seung S Lee, Lenore K Beitel, Matthew L Carnevale, Miltiadis Paliouras, Andrew G Kirk, Mark A Trifiro
Polymerase Chain Reaction (PCR) is a critical tool for biological research investigators but recently it also has been making a significant impact in clinical, veterinary and agricultural applications. Plasmonic PCR, which employs the very efficient heat transfer of optically irradiated metallic nanoparticles, is a simple and powerful methodology to drive PCR reactions. The scalability of next generation plasmonic PCR technology will introduce various forms of PCR applications ranging from small footprint portable point of care diagnostic devices to large footprint central laboratory multiplexing devices...
April 26, 2017: Analyst
https://www.readbyqxmd.com/read/28439938/intramuscular-fat-infiltration-evaluated-by-magnetic-resonance-imaging-predicts-the-extensibility-of-the-supraspinatus-muscle
#2
Hugo Giambini, Taku Hatta, Gorny R Krzysztof, Per Widholm, Anette Karlsson, Olof D Leinhard, Mark C Adkins, Chunfeng Zhao, Kai-Nan An
INTRODUCTION: Rotator cuff (RC) tears result in muscle atrophy and fat infiltration within the RC muscles. An estimation of muscle quality and deformation, or extensibility, is useful in selecting the most appropriate surgical procedure. We determined if non-invasive quantitative assessment of intramuscular fat using MRI could be used to predict extensibility of the supraspinatus (SSP) muscle. METHODS: Seventeen cadaveric shoulders were imaged to assess intramuscular fat infiltration...
April 25, 2017: Muscle & Nerve
https://www.readbyqxmd.com/read/28439033/genome-wide-identification-of-resd-nsrr-and-fur-binding-in-bacillus-subtilis-during-anaerobic-fermentative-growth-by-in-vivo-footprinting
#3
Onuma Chumsakul, Divya P Anantsri, Tai Quirke, Taku Oshima, Kensuke Nakamura, Shu Ishikawa, Michiko M Nakano
Upon oxygen limitation, the Bacillus subtilis ResE sensor kinase and its cognate ResD response regulator play primary roles in transcriptional activation of genes functioning in anaerobic respiration. The nitric oxide (NO)-sensitive NsrR repressor controls transcription to support nitrate respiration. In addition, the ferric uptake repressor (Fur) can modulate transcription under anaerobic conditions. However, whether these controls are direct or indirect has been investigated only in a gene-specific manner...
April 24, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28438268/fungal-biorecovery-of-gold-from-e-waste
#4
Saskia Bindschedler, Thi Quynh Trang Vu Bouquet, Daniel Job, Edith Joseph, Pilar Junier
Waste electric and electronic devices (e-waste) represent a source of valuable raw materials of great interest, and in the case of metals, e-waste might become a prized alternative source. Regarding gold, natural ores are difficult to mine due to their refractory nature and the richest ores have almost all been exploited. Additionally, some gold mining areas are present in geopolitically unstable regions. Finally, the gold mining industry produces toxic compounds, such as cyanides. As a result, the gold present in e-waste represents a nonnegligible resource (urban mining)...
2017: Advances in Applied Microbiology
https://www.readbyqxmd.com/read/28437989/low-loss-compact-and-fabrication-tolerant-si-wire-90%C3%A2-waveguide-bend-using-clothoid-and-normal-curves-for-large-scale-photonic-integrated-circuits
#5
Takeshi Fujisawa, Shuntaro Makino, Takanori Sato, Kunimasa Saitoh
Ultimately low-loss 90° waveguide bend composed of clothoid and normal curves is proposed for dense optical interconnect photonic integrated circuits. By using clothoid curves at the input and output of 90° waveguide bend, straight and bent waveguides are smoothly connected without increasing the footprint. We found that there is an optimum ratio of clothoid curves in the bend and the bending loss can be significantly reduced compared with normal bend. 90% reduction of the bending loss for the bending radius of 4 μm is experimentally demonstrated with excellent agreement between theory and experiment...
April 17, 2017: Optics Express
https://www.readbyqxmd.com/read/28437684/fibrillar-disruption-by-ac-electric-field-induced-oscillation-a-case-study-with-human-serum-albumin
#6
Shubhatam Sen, Monojit Chakraborty, Snigdha Goley, Swagata Dasgupta, Sunando DasGupta
The effect of oscillation induced by a frequency-dependent alternating current (AC) electric field to dissociate preformed amyloid fibrils has been investigated. An electrowetting-on-dielectric type setup has been used to apply the AC field of varying frequencies on preformed fibrils of human serum albumin (HSA). The disintegration potency has been monitored by a combination of spectroscopic and microscopic techniques. The experimental results suggest that the frequency of the applied AC field plays a crucial role in the disruption of preformed HSA fibrils...
April 13, 2017: Biophysical Chemistry
https://www.readbyqxmd.com/read/28436877/packing-vertex-data-into-hardware-decompressible-textures
#7
Kin Chung Kwan, Xuemiao Xu, Liang Wan, Tien-Tsin Wong, Wai-Man Pang
Most graphics hardware features memory to store textures and vertex data for rendering. However, because of the irreversible trend of increasing complexity of scenes, rendering a scene can easily reach the limit of memory resources. Thus, vertex data are preferably compressed, with a requirement that they can be decompressed during rendering. In this paper, we present a novel method to exploit existing hardware texture compression circuits to facilitate the decompression of vertex data in graphics processing unit (GPUs)...
April 18, 2017: IEEE Transactions on Visualization and Computer Graphics
https://www.readbyqxmd.com/read/28435669/reframing-the-science-and-policy-of-nicotine-illegal-drugs-and-alcohol-conclusions-of-the-alice-rap-project
#8
Peter Anderson, Virginia Berridge, Patricia Conrod, Robert Dudley, Matilda Hellman, Dirk Lachenmeier, Anne Lingford-Hughes, David Miller, Jürgen Rehm, Robin Room, Laura Schmidt, Roger Sullivan, Tamyko Ysa, Antoni Gual
In 2013, illegal drug use was responsible for 1.8% of years of life lost in the European Union, alcohol was responsible for 8.2% and tobacco for 18.2%, imposing economic burdens in excess of 2.5% of GDP. No single European country has optimal governance structures for reducing the harm done by nicotine, illegal drugs and alcohol, and existing ones are poorly designed, fragmented, and sometimes cause harm. Reporting the main science and policy conclusions of a transdisciplinary five-year analysis of the place of addictions in Europe, researchers from 67 scientific institutions addressed these problems by reframing an understanding of addictions...
2017: F1000Research
https://www.readbyqxmd.com/read/28432532/a-portable-battery-powered-microfluidic-impedance-cytometer-with-smartphone-readout-towards-personal-health-monitoring
#9
Niloy Talukder, Abbas Furniturewalla, Tuan Le, Matthew Chan, Shreyas Hirday, Xinnan Cao, Pengfei Xie, Zhongtian Lin, Azam Gholizadeh, Steve Orbine, Mehdi Javanmard
We present a portable system for personalized blood cell counting consisting of a microfluidic impedance cytometer and portable analog readout electronics, feeding into an analog-to-digital converter (ADC), and being transmitted via Bluetooth to a user-accessible mobile application. We fabricated a microfluidic impedance cytometer with a novel portable analog readout. The novel design of the analog readout, which consists of a lock-in-amplifier followed by a high-pass filter stage for subtraction of drift and DC offset, and a post-subtraction high gain stage, enables detection of particles and cells as small as 1 μm in diameter, despite using a low-end 8-bit ADC...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28429828/genomic-clustering-of-adaptive-loci-during-parallel-evolution-of-an-australian-wildflower
#10
Federico Roda, Greg M Walter, Rick Nipper, Daniel Ortiz-Barrientos
The buildup of the phenotypic differences that distinguish species has long intrigued biologists. These differences are often inherited as stable polymorphisms that allow the co-segregation of adaptive variation within species, and facilitate the differentiation of complex phenotypes between species. It has been suggested that the clustering of adaptive loci could facilitate this process but evidence is still scarce. Here we used QTL analysis to study the genetic basis of phenotypic differentiation between coastal populations of the Australian wildflower Senecio lautus...
April 21, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28428820/regional-water-footprints-of-potential-biofuel-production-in-china
#11
Xiaomin Xie, Tingting Zhang, Liming Wang, Zhen Huang
BACKGROUND: Development of biofuels is considered as one of the important ways to replace conventional fossil energy and mitigate climate change. However, rapid increase of biofuel production could cause other environmental concerns in China such as water stress. This study is intended to evaluate the life-cycle water footprints (WF) of biofuels derived from several potential non-edible feedstocks including cassava, sweet sorghum, and Jatropha curcas in China. Different water footprint types including blue water, green water, and grey water are considered in this study...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28428164/zika-in-twitter-temporal-variations-of-locations-actors-and-concepts
#12
Anthony Stefanidis, Emily Vraga, Georgios Lamprianidis, Jacek Radzikowski, Paul L Delamater, Kathryn H Jacobsen, Dieter Pfoser, Arie Croitoru, Andrew Crooks
BACKGROUND: The recent Zika outbreak witnessed the disease evolving from a regional health concern to a global epidemic. During this process, different communities across the globe became involved in Twitter, discussing the disease and key issues associated with it. This paper presents a study of this discussion in Twitter, at the nexus of location, actors, and concepts. OBJECTIVE: Our objective in this study was to demonstrate the significance of 3 types of events: location related, actor related, and concept related, for understanding how a public health emergency of international concern plays out in social media, and Twitter in particular...
April 20, 2017: JMIR Public Health and Surveillance
https://www.readbyqxmd.com/read/28427872/abduction-brace-versus-antirotation-sling-after-arthroscopic-cuff-repair-the-effects-on-pain-and-function
#13
Freek Hollman, Nienke Wolterbeek, Jacco A C Zijl, Sjoerd P M van Egeraat, Ronald N Wessel
PURPOSE: To study the effects on pain as the main outcome parameter and on function and cuff integrity as the secondary outcome parameters after arthroscopic rotator cuff repair in the short term comparing the abduction brace with an antirotation sling for postoperative shoulder immobilization. METHODS: Eligible patients were between the ages of 18 and 75 years who were diagnosed with a traumatic or degenerative tear of the supraspinatus and/or infraspinatus tendon, confirmed by magnetic resonance imaging, for which an arthroscopic footprint repair was indicated and performed...
April 17, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28427532/nanotechnology-for-food-packaging-and-food-quality-assessment
#14
Marco Rossi, Daniele Passeri, Alberto Sinibaldi, Mariglen Angjellari, Emanuela Tamburri, Angela Sorbo, Elisabetta Carata, Luciana Dini
Nanotechnology has paved the way to innovative food packaging materials and analytical methods to provide the consumers with healthier food and to reduce the ecological footprint of the whole food chain. Combining antimicrobial and antifouling properties, thermal and mechanical protection, oxygen and moisture barrier, as well as to verify the actual quality of food, e.g., sensors to detect spoilage, bacterial growth, and to monitor incorrect storage conditions, or anticounterfeiting devices in food packages may extend the products shelf life and ensure higher quality of foods...
2017: Advances in Food and Nutrition Research
https://www.readbyqxmd.com/read/28426987/a-review-on-nitrous-oxide-n2o-emissions-during-biological-nutrient-removal-from-municipal-wastewater-and-sludge-reject-water
#15
REVIEW
Theoni Maria Massara, Simos Malamis, Albert Guisasola, Juan Antonio Baeza, Constantinos Noutsopoulos, Evina Katsou
Nitrous oxide (N2O) is an important pollutant which is emitted during the biological nutrient removal (BNR) processes of wastewater treatment. Since it has a greenhouse effect which is 265 times higher than carbon dioxide, even relatively small amounts can result in a significant carbon footprint. Biological nitrogen (N) removal conventionally occurs with nitrification/denitrification, yet also through advanced processes such as nitritation/denitritation and completely autotrophic N-removal. The microbial pathways leading to the N2O emission include hydroxylamine oxidation and nitrifier denitrification, both activated by ammonia oxidizing bacteria, and heterotrophic denitrification...
April 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28424707/new-insights-on-leucine-rich-repeats-receptor-like-kinase-orthologous-relationships-in-angiosperms
#16
Jean-François Dufayard, Mathilde Bettembourg, Iris Fischer, Gaetan Droc, Emmanuel Guiderdoni, Christophe Périn, Nathalie Chantret, Anne Diévart
Leucine-Rich Repeats Receptor-Like Kinase (LRR-RLK) genes represent a large and complex gene family in plants, mainly involved in development and stress responses. These receptors are composed of an LRR-containing extracellular domain (ECD), a transmembrane domain (TM) and an intracellular kinase domain (KD). To provide new perspectives on functional analyses of these genes in model and non-model plant species, we performed a phylogenetic analysis on 8,360 LRR-RLK receptors in 31 angiosperm genomes (8 monocots and 23 dicots)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28423005/an-archosauromorph-dominated-ichnoassemblage-in-fluvial-settings-from-the-late-early-triassic-of-the-catalan-pyrenees-ne-iberian-peninsula
#17
Eudald Mujal, Josep Fortuny, Arnau Bolet, Oriol Oms, José Ángel López
The vertebrate recovery after the end-Permian mass extinction can be approached through the ichnological record, which is much more abundant than body fossils. The late Olenekian (Early Triassic) tetrapod ichnoassemblage of the Catalan Pyrenean Basin is the most complete and diverse of this age from Western Tethys. This extensional basin, composed of several depocenters, was formed in the latest phases of the Variscan orogeny (Pangea breakup) and was infilled by braided and meandering fluvial systems of the red-beds Buntsandstein facies...
2017: PloS One
https://www.readbyqxmd.com/read/28422964/effect-of-women-s-age-on-embryo-morphology-cleavage-rate-and-competence-a-multicenter-cohort-study
#18
MULTICENTER STUDY
Marie Louise Grøndahl, Sofie Lindgren Christiansen, Ulrik Schiøler Kesmodel, Inge Errebo Agerholm, Josephine Gabriela Lemmen, Peter Lundstrøm, Jeanette Bogstad, Morten Raaschou-Jensen, Steen Ladelund
This multicenter cohort study on embryo assessment and outcome data from 11,744 IVF/ICSI cycles with 104,830 oocytes and 42,074 embryos, presents the effect of women's age on oocyte, zygote, embryo morphology and cleavage parameters, as well as cycle outcome measures corrected for confounding factors as center, partner's age and referral diagnosis. Cycle outcome data confirmed the well-known effect of women's age. Oocyte nuclear maturation and proportion of 2 pro-nuclear (2PN) zygotes were not affected by age, while a significant increase in 3PN zygotes was observed in both IVF and ICSI (p<0...
2017: PloS One
https://www.readbyqxmd.com/read/28419537/electromagnetic-tracking-for-femoral-derotation-osteotomy-an-in-vivo-study
#19
Andreas Geisbüsch, Christoph Auer, Hartmut Dickhaus, Cornelia Putz, Thomas Dreher
Femoral derotation osteotomy delivers good to excellent results in the treatment of rotational gait abnormalities and especially in internal rotation gait. The outcome of the procedure has been evaluated in numerous short and long term studies. Although reasons for recurrence and over-/under-correction have been unveiled in earlier studies the mechanisms are still not fully understood. False intra-operative assessment of the derotation angle may contribute to imprecise outcomes. In a recent saw-bone study we evaluated an electromagnetic tracking system in comparison to conventional goniometer measurement and a CT reference measurement and found it to be extremely accurate, whereas the use of a conventional goniometer for derotation measurement showed a high inter- and intra-rater variability...
April 17, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28419194/dminda-2-0-integrated-and-systematic-views-of-regulatory-dna-motif-identification-and-analyses
#20
Jinyu Yang, Xin Chen, Adam McDermaid, Qin Ma
Motivation: Motif identification and analyses are important and have been long-standing computational problems in bioinformatics. Substantial efforts have been made in this field during the past several decades. However, the lack of intuitive and integrative web servers impedes the progress of making effective use of emerging algorithms and tools. Results: Here we present an integrated web server, DMINDA 2.0, which contains: (i) five motif prediction and analyses algorithms, including a phylogenetic footprinting framework; (ii) 2,125 species with complete genomes to support the above five functions, covering animals, plants, and bacteria; and (iii) bacterial regulon prediction and visualization...
April 13, 2017: Bioinformatics
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