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https://www.readbyqxmd.com/read/28820333/protease-dead-separase-inhibits-chromosome-segregation-and-rab-11-vesicle-trafficking
#1
Xiaofei Bai, Joshua N Bembenek
Separase cleaves cohesin to allow chromosome segregation. Separase also regulates cortical granule exocytosis and vesicle trafficking during cytokinesis, both of which involve RAB-11. We investigated whether separase regulates exocytosis through a proteolytic or non-proteolytic mechanism. In C. elegans, protease-dead separase (SEP-1(PD)::GFP) is dominant negative. Consistent with its role in cohesin cleavage, SEP-1(PD)::GFP causes chromosome segregation defects. As expected, partial depletion of cohesin rescues this defect, confirming that SEP-1(PD)::GFP acts through a substrate trapping mechanism...
August 18, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28808062/ablating-tbc1d1-impairs-contraction-induced-sarcolemmal-glucose-transporter-type-4-redistribution-but-not-insulin-mediated-responses-in-rats
#2
Jamie Whitfield, Sabina Paglialunga, Brennan K Smith, Paula M Miotto, Genevieve Simnett, Holly L Robson, Swati S Jain, Eric A F Herbst, Eric M Desjardins, David J Dyck, Lawrence L Spriet, Gregory R Steinberg, Graham P Holloway
TBC1 domain family member 1 (TBC1D1), a Rab GTPase-activating protein and paralogue of Akt substrate of 160 kDa (AS160), has been implicated in both insulin- and 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICAR)-mediated glucose transporter type (GLUT4) translocation. However, the role of TBC1D1 in contracting muscle remains ambiguous. We therefore explored the metabolic consequence of ablating TBC1D1 in both resting and contracting skeletal muscles, utilizing a rat TBC1D1-KO model...
August 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28803608/identification-of-simvastatin-regulated-targets-associated-with-jnk-activation-in-du145-human-prostate-cancer-cell-death-signaling
#3
Eun Joo Jung, Ky Hyun Chung, Choong Won Kim
In this study, our results showed that the c-Jun N-terminal kinase (JNK) activation was associated with the enhancement of docetaxel-induced cytotoxicity by simvastatin in DU145 human prostate cancer cells. To better understand the basic molecular mechanisms, we investigated simvastatin-regulated targets during simvastatin-induced cell death in DU145 cells using two-dimensional (2D) proteomic analysis. Thus, vimentin, Ras-related protein Rab-1B (RAB1B), cytoplasmic hydroxymethylglutaryl-CoA synthase (cHMGCS), thioredoxin domain-containing protein 5 (TXNDC5), heterogeneous nuclear ribonucleoprotein K (hnRNP K), N-myc downstream-regulated gene 1 (NDRG1) and isopentenyl-diphosphate Delta-isomerase 1 (IDI1) protein spots were identified as simvastatin-regulated targets involved in DU145 cell death signaling pathways...
August 11, 2017: BMB Reports
https://www.readbyqxmd.com/read/28791425/rab44-a-novel-large-rab-gtpase-negatively-regulates-osteoclast-differentiation-by-modulating-intracellular-calcium-levels-followed-by-nfatc1-activation
#4
Yu Yamaguchi, Eiko Sakai, Kuniaki Okamoto, Hiroshi Kajiya, Koji Okabe, Mariko Naito, Tomoko Kadowaki, Takayuki Tsukuba
Rab44 is an atypical Rab GTPase that contains some additional domains such as the EF-hand and coiled-coil domains as well as Rab-GTPase domain. Although Rab44 genes have been found in mammalian genomes, no studies concerning Rab44 have been reported yet. Here, we identified Rab44 as an upregulated protein during osteoclast differentiation. Knockdown of Rab44 by small interfering RNA promotes RANKL-induced osteoclast differentiation of the murine monocytic cell line, RAW-D or of bone marrow-derived macrophages (BMMs)...
August 8, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28774736/rep1-copy-number-variation-is-an-important-genetic-cause-of-choroideremia-in-chinese-patients
#5
Qi Zhou, Fengxia Yao, Xiaoxu Han, Hui Li, Lizhu Yang, Ruifang Sui
Choroidermia (CHM) is an X-linked chorioretinal disorder caused by mutations in the Rab Escort Protein 1 (Rep-1) gene. Its diagnosis depends on clinical findings and genetic confirmation; however, mutations in Rep-1 gene are not always detected by standard Sanger sequencing. We therefore conducted multiplex ligation-dependent probe amplification (MLPA) and real-time quantitative PCR (QPCR) in cases of Chinese CHM families in which sequencing all the exons and flanking intronic regions of the CHM gene had not identified a mutation or exons could not be amplified...
July 31, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28761819/legionella-pneumophila-and-dendrimers-mediated-antisense-therapy
#6
REVIEW
Roghiyeh Pashaei-Asl, Khodadad Khodadadi, Fatima Pashaei-Asl, Gholamreza Haqshenas, Nasser Ahmadian, Maryam Pashaiasl, Reza Hajihosseini Baghdadabadi
Finding novel and effective antibiotics for treatment of Legionella disease is a challenging field. Treatment with antibiotics usually cures Legionella infection; however, if the resultant disease is not timely recognized and treated properly, it leads to poor prognosis and high case fatality rate. Legionella pneumophila DrrA protein (Defects in Rab1 recruitment protein A)/also known as SidM affects host cell vesicular trafficking through modification of the activity of cellular small guanosine triphosphatase )GTPase( Rab (Ras-related in brain) function which facilitates intracellular bacterial replication within a supporter vacuole...
June 2017: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28761200/a-prospective-study-of-altered-inflammatory-response-and-its-clinical-outcome-following-laparoscopic-and-open-cholecystectomy
#7
Sayyed Ehtesham Hussain Naqvi, Atia Zaka-Ur-Rab, Najmul Islam, Eram Ali
BACKGROUND: Inflammatory response following surgical trauma has long been a matter of study. Results, however, have been varied. We sought to assess changes in the levels of proinflammatory and anti-inflammatory cytokines in patients undergoing laparoscopic and open cholecystectomy and their impact on the clinical outcome of patients concerning the postoperative pain score. METHODS: The study involved 90 cholecystectomies (55 laparoscopic and 35 open) for chronic cholecystitis...
July 2017: Iranian Journal of Medical Sciences
https://www.readbyqxmd.com/read/28755404/the-dense-core-vesicle-maturation-protein-cccp-1-binds-rab-2-and-membranes-through-its-c-terminal-domain
#8
Jérôme Cattin-Ortolá, Irini Topalidou, Annie Dosey, Alexey J Merz, Michael Ailion
Dense-core vesicles (DCVs) are secretory organelles that store and release modulatory neurotransmitters from neurons and endocrine cells. Recently, the conserved coiled-coil protein CCCP-1 was identified as a component of the DCV biogenesis pathway in the nematode C. elegans. CCCP-1 binds the small GTPase RAB-2 and colocalizes with it at the trans-Golgi. Here we report a structure-function analysis of CCCP-1 to identify domains of the protein important for its localization, binding to RAB-2, and function in DCV biogenesis...
July 28, 2017: Traffic
https://www.readbyqxmd.com/read/28751526/the-salivary-peptide-histatin-1-promotes-endothelial-cell-adhesion-migration-and-angiogenesis
#9
Pedro Torres, Jorge Díaz, Maximiliano Arce, Patricio Silva, Pablo Mendoza, Pablo Lois, Alfredo Molina-Berríos, Gareth I Owen, Verónica Palma, Vicente A Torres
Saliva is a key factor that contributes to the high efficiency of wound healing in the oral mucosa. This is not only attributed to physical cues but also to the presence of specific peptides in the saliva, such as histatins. Histatin-1 is a 38 aa antimicrobial peptide, highly enriched in human saliva, which has been previously reported to promote the migration of oral keratinocytes and fibroblasts in vitro However, the participation of histatin-1 in other crucial events required for wound healing, such as angiogenesis, is unknown...
July 27, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28751423/sid-1-functions-in-multiple-roles-to-support-parental-rnai-in-caenorhabditis-elegans
#10
Eddie Wang, Craig P Hunter
Systemic RNA interference (RNAi) in Caenorhbaditis elegans requires sid-1, sid-3, and sid-5 Injected, expressed, or ingested double-stranded RNA (dsRNA) is transported between cells, enabling RNAi in most tissues, including the germline and progeny (parental RNAi). A recent report claims that parental RNAi also requires the yolk receptor rme-2 Here, we characterize the role of the sid genes and rme-2 in parental RNAi. We identify multiple independent paths for maternal dsRNA to reach embryos and initiate RNAi...
July 27, 2017: Genetics
https://www.readbyqxmd.com/read/28743200/green-fluorescent-protein-gfp-is-seeing-believing-and-is-that-enough
#11
Susan A Shorter, Marie W Pettit, Paul D R Dyer, J Emma Coakley-Youngs, Monique A M Gorringe-Pattrick, Samer El-Daher, Simon C W Richardson
Intracellular compartmentalisation is a significant barrier to the successful nucleocytosolic delivery of biologics. The endocytic system has been shown to be responsible for compartmentalisation, providing an entry point, and trigger(s) for the activation of drug delivery systems. Consequently, many of the technologies used to understand endocytosis have found utility within the field of drug delivery. The use of fluorescent proteins as markers denoting compartmentalisation within the endocytic system has become commonplace...
July 26, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28739266/identification-of-%C3%AE-helix-4-%C3%AE-4-of-rab11a-as-a-novel-rab11-binding-domain-rbd-interaction-of-rab11a-with-the-prostacyclin-receptor
#12
Eamon P Mulvaney, Fergal O'Meara, Amir R Khan, David J O'Connell, B Therese Kinsella
The cellular trafficking of numerous G protein-coupled receptors (GPCRs) is known to be regulated by Rab proteins that involves a direct protein:protein interaction between the receptor and the GTPase. In the case of the human prostacyclin receptor (hIP), it undergoes agonist-induced internalization and subsequent Rab11a-dependent recyclization involving an interaction between a Rab11-binding domain (RBD) localized within its carboxyl-tail domain with Rab11a. However, the GPCR-interacting domain on Rab11a itself is unknown...
July 22, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28735522/characterization-of-the-role-of-rilp-in-cell-migration
#13
Azzurra Margiotta, Cinzia Progida, Oddmund Bakke, Cecilia Bucci
Rab-interacting lysosomal protein (RILP) is a regulator of late stages of endocytosis. Recent work proved that depletion of RILP promotes migration of breast cancer cells in wound healing assay, whereas its overexpression influences re-arrangements of actin cytoskeleton. Here, we further characterized the role of RILP in cell migration by analyzing several aspects of this process. We showed that RILP is fundamental also for migration of lung cancer cells regulating cell velocity. RILP silencing did not affect Golgi apparatus nor microtubules reorientation during migration...
May 31, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28726776/autophagosome-maturation-mediated-by-rab7-contributes-to-neuroprotection-of-hypoxic-preconditioning-against-global-cerebral-ischemia-in-rats
#14
Lixuan Zhan, Siyuan Chen, Kongping Li, Donghai Liang, Xinyong Zhu, Liu Liu, Zhiwei Lu, Weiwen Sun, En Xu
Autophagy disruption leads to neuronal damage in hypoxic-ischemic brain injury. Rab7, a member of the Rab GTPase superfamily, has a unique role in the regulation of autophagy. Hypoxic preconditioning (HPC) provides neuroprotection against transient global cerebral ischemia (tGCI). However, the underlying mechanisms remain poorly understood. Thus, the current study explored the potential molecular mechanism of the neuroprotective effect of HPC by investigating how Rab7 mediates autophagosome (AP) maturation after tGCI in adult rats...
July 20, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28724764/rab5-and-rab11-are-required-for-clathrin-dependent-endocytosis-of-japanese-encephalitis-virus-in-bhk-21-cells
#15
Chun-Chun Liu, Yun-Na Zhang, Zhao-Yao Li, Jin-Xiu Hou, Jing Zhou, Lin Kan, Bin Zhou, Pu-Yan Chen
During infection Japanese Encephalitis Virus generally enters host cells via receptor-mediated clathrin dependent endocytosis. The trafficking of JEV within endosomes is controlled by Rab GTPases, but which Rab proteins are involved in JEV entry into BHK-21 cells is unknown. In this study, entry and post-internalization of JEV were analyzed using biochemical inhibitors, RNA interference and dominant negative mutants. Our data demonstrate that JEV entry into BHK-21 cells depends on clathrin, dynamin, and cholesterol, but not caveolae or macropinocytosis...
July 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28717323/epidemiological-study-of-tick-infestation-in-buffalo-of-various-regions-of-district-khairpur-pakistan
#16
Farzana Abbasi, Imtiaz Hussain Raja Abbasi, Tahmeena Fakhur Nissa, Zohaib Ahmed Bhutto, Muhammed Asif Arain, Rab Nawaz Soomro, Farman Ali Siyal, Sarfraz Ali Fazlani
AIM: The aim of this study was to determine the epidemiological infestation and identification of Ixodidae and Argasidae ticks species in buffalo of different parts of district Khairpur, Pakistan. MATERIALS AND METHODS: A total of 720 Water buffaloes from three tehsils (subdivisions) were selected randomly and examined from organized and unorganized dairy farms for tick infestation in district Khairpur, Pakistan. This epidemiological survey was conducted during April to September 2015...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28707374/localization-and-functional-analysis-of-the-insect-specific-rabx4-in-the-brain-of-bombyx-mori
#17
Tomohide Uno, Masayuki Furutani, Katsuhiko Sakamoto, Yuichi Uno, Kengo Kanamaru, Akira Mizoguchi, Susumu Hiragaki, Makio Takeda
Rab proteins are small monomeric GTPases/GTP-binding proteins, which form the largest branch of the Ras superfamily. The different Rab GTPases are localized to the cytosolic face of specific intracellular membranes, where they function as regulators of distinct steps in membrane trafficking. RabX4 is an insect-specific Rab protein that has no close homolog in vertebrates. There is little information about insect-specific Rab proteins. RabX4 was expressed in Escherichia coli and subsequently purified. Antibodies against Bombyx mori RabX4 were produced in rabbits for western immunoblotting and immunohistochemistry...
July 14, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28696821/multifaceted-role-of-smcr8-as-autophagy-regulator
#18
Jennifer Jung, Christian Behrends
Through autophagy intracellular material is engulfed by double membrane vesicles and delivered to lysosomes for degradation. This process requires Rab GTPases, Rab GAPs and Rab GEFs for proper membrane trafficking, since they control vesicle budding, targeting and fusion. Deregulation of autophagy contributes to several human diseases including cancer, bacterial or viral infections and neurodegeneration. This review focuses on the complex roles of the newly identified protein SMCR8 and its interaction partners during formation and maturation of autophagosomes as well as regulation of lysosomal function and further discusses their implication in neurodegenerative diseases such as ALS and FTD...
July 11, 2017: Small GTPases
https://www.readbyqxmd.com/read/28696820/viral-interactions-with-host-cell-rab-gtpases
#19
Paul Spearman
Viruses are obligate intracellular parasites that utilize cellular machinery for many aspects of their replication cycles. Enveloped viruses generally rely upon host vesicular trafficking machinery to direct their structural proteins and genomes to sites of virus replication, assembly, and budding. Rab GTPases have been implicated in the replication of many important viral pathogens infecting humans. This review provides a summary of virus-Rab protein interactions, with a particular focus on the role of Rab-related trafficking pathways on late events in the lifecycle of herpesviruses and of HIV-1...
July 11, 2017: Small GTPases
https://www.readbyqxmd.com/read/28686766/evaluation-of-patch-test-reactivities-in-patients-with-chronic-idiopathic-urticaria
#20
Khalid M AlGhamdi, Huma Khurrum, Mohamed O Gad Al Rab
Chronic idiopathic urticaria (CIU) is one of the mysteries of dermatology. An association between spontaneous CIU and contact allergy sensitization is possible. In this study, we aimed to test the hypothesis that contact allergy might play a role in disease in CIU patients. Furthermore, we examined if patch testing should be routinely performed in patients with chronic urticaria (CU). Ultimately, we concluded that patch testing should be included among the diagnostic procedures for CU.
June 2017: Cutis; Cutaneous Medicine for the Practitioner
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