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BCAA metabolism

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https://www.readbyqxmd.com/read/29324714/determination-of-branched-chain-keto-acids-in-serum-and-muscles-using-high-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#1
You Zhang, Bingjie Yin, Runxian Li, Pingli He
Branched-chain keto acids (BCKAs) are derivatives from the first step in the metabolism of branched-chain amino acids (BCAAs) and can provide important information on animal health and disease. Here, a simple, reliable and effective method was developed for the determination of three BCKAs (α-ketoisocaproate, α-keto-β-methylvalerate and α-ketoisovalerate) in serum and muscle samples using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS). The samples were extracted using methanol and separated on a 1...
January 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29307017/clinical-characteristics-and-mutation-analysis-of-five-chinese-patients-with-maple-syrup-urine-disease
#2
Xiaomei Li, Yali Yang, Qing Gao, Min Gao, Yvqiang Lv, Rui Dong, Yi Liu, Kaihui Zhang, Zhongtao Gai
Maple syrup urine disease (MSUD) is an autosomal recessive disorder affecting branched-chain amino acids (BCAAs) metabolism and caused by a defect in the thiamine-dependent enzyme branched chain α-ketoacid dehydrogenase (BCKD) with subsequent accumulation of BCAAs and corresponding branched-chain keto acids (BCKAs) metabolites. Presently, at least 4 genes of BCKDHA, BCKDHB, DLD and DBT have been reported to cause MSUD. Furthermore, more than 265 mutations have been identified as the cause across different populations worldwide...
January 6, 2018: Metabolic Brain Disease
https://www.readbyqxmd.com/read/29288718/kr%C3%A3-ppel-like-factor-15-regulator-of-bcaa-metabolism-and-circadian-protein-rhythmicity
#3
REVIEW
Liyan Fan, Paishiun N Hsieh, David R Sweet, Mukesh K Jain
Regulation of nutrient intake, utilization, and storage exhibits a circadian rhythmicity that allows organisms to anticipate and adequately respond to changes in the environment across day/night cycles. The branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are important modulators of metabolism and metabolic health - for example, their catabolism yields carbon substrates for gluconeogenesis during periods of fasting. Krüppel-like factor 15 (KLF15) has recently emerged as a critical transcriptional regulator of BCAA metabolism, and the absence of this transcription factor contributes to severe pathologies such as Duchenne muscular dystrophy and heart failure...
December 27, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29281182/high-dietary-intakes-of-branched-chain-amino-acids-is-associated-with-increased-risk-of-insulin-resistance-in-adults
#4
Golaleh Asghari, Hossein Farhadnejad, Farshad Teymoori, Parvin Mirmiran, Maryam Tohidi, Fereidoun Azizi
BACKGROUND: The aim of this study was to investigate the association between branched-chain amino acids (BCAAs) intake and markers of insulin metabolism in adults. METHODS: This cohort study was conducted within the framework of the Tehran Lipid and Glucose Study (TLGS), on 1205 subjects, aged ≥ 20 years, who followed-up for a mean duration of 2.3 years. Dietary intakes of BCAAs including valine, leucine, and isoleucine were determined, using a valid and reliable food-frequency questionnaire...
December 27, 2017: Journal of Diabetes
https://www.readbyqxmd.com/read/29246479/elevated-plasma-levels-of-3-hydroxyisobutyric-acid-are-associated-with-incident-type-2-diabetes
#5
Adil Mardinoglu, Silvia Gogg, Luca A Lotta, Alena Stančáková, Annika Nerstedt, Jan Boren, Matthias Blüher, Ele Ferrannini, Claudia Langenberg, Nicholas J Wareham, Markku Laakso, Ulf Smith
Branched-chain amino acids (BCAAs) metabolite, 3-Hydroxyisobutyric acid (3-HIB) has been identified as a secreted mediator of endothelial cell fatty acid transport and insulin resistance (IR) using animal models. To identify if 3-HIB is a marker of human IR and future risk of developing Type 2 diabetes (T2D), we measured plasma levels of 3-HIB and associated metabolites in around 10,000 extensively phenotyped individuals. The levels of 3-HIB were increased in obesity but not robustly associated with degree of IR after adjusting for BMI...
December 7, 2017: EBioMedicine
https://www.readbyqxmd.com/read/29221133/endogenous-glutamine-decrease-is-associated-with-pancreatic-cancer-progression
#6
Cecilia Roux, Chiara Riganti, Sammy Ferri Borgogno, Roberta Curto, Claudia Curcio, Valeria Catanzaro, Giuseppe Digilio, Sergio Padovan, Maria Paola Puccinelli, Monica Isabello, Silvio Aime, Paola Cappello, Francesco Novelli
Pancreatic ductal adenocarcinoma (PDAC) is becoming the second leading cause of cancer-related death in the Western world. The mortality is very high, which emphasizes the need to identify biomarkers for early detection. As glutamine metabolism alteration is a feature of PDAC, its in vivo evaluation may provide a useful tool for biomarker identification. Our aim was to identify a handy method to evaluate blood glutamine consumption in mouse models of PDAC. We quantified the in vitro glutamine uptake by Mass Spectrometry (MS) in tumor cell supernatants and showed that it was higher in PDAC compared to non-PDAC tumor and pancreatic control human cells...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29211698/branched-chain-amino-acid-metabolism-in-cancer
#7
Elitsa A Ananieva, Adam C Wilkinson
PURPOSE OF REVIEW: The current review aims to provide an update on the recent biomedical interest in oncogenic branched-chain amino acid (BCAA) metabolism, and discusses the advantages of using BCAAs and expression of BCAA-related enzymes in the treatment and diagnosis of cancers. RECENT FINDINGS: An accumulating body of evidence demonstrates that BCAAs are essential nutrients for cancer growth and are used by tumors in various biosynthetic pathways and as a source of energy...
January 2018: Current Opinion in Clinical Nutrition and Metabolic Care
https://www.readbyqxmd.com/read/29209542/skin-lesions-associated-with-nutritional-management-of-maple-syrup-urine-disease
#8
Jaraspong Uaariyapanichkul, Puthita Saengpanit, Ponghatai Damrongphol, Kanya Suphapeetiporn, Sirinuch Chomtho
Introduction: Maple syrup urine disease (MSUD) is an inborn error of branched chain amino acids (BCAAs) metabolism. We report an infant with MSUD who developed 2 episodes of cutaneous lesions as a result of isoleucine deficiency and zinc deficiency, respectively. Case Presentation: A 12-day-old male infant was presented with poor milk intake and lethargy. The diagnosis of MSUD was made based on clinical and biochemical data. Management and Outcome: Specific dietary restriction of BCAAs was given...
2017: Case Reports in Dermatological Medicine
https://www.readbyqxmd.com/read/29198954/metabolic-reprogramming-of-hydrogenosomal-amino-acids-in-trichomonas-vaginalis-under-glucose-restriction
#9
Kuo-Yang Huang, Seow-Chin Ong, Chih-Ching Wu, Chia-Wei Hsu, Hsin-Chung Lin, Yi-Kai Fang, Wei-Hung Cheng, Po-Jung Huang, Cheng-Hsun Chiu, Petrus Tang
BACKGROUND: Glucose is the major energy source that is converted to pyruvate for ATP generation in the trichomonad hydrogenosome. Under glucose restriction (GR), the regulation of amino acids metabolism is crucial for trichomonad growth and survival. RNA-sequencing (RNA-seq) analysis has been used to identify differentially expressed genes in Trichomonas vaginalis under GR, leading to significant advances in understanding adaptive responses of amino acid metabolism to GR. However, the levels of amino acid metabolites modulated by GR are unknown in T...
November 21, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29197337/genome-wide-motif-predictions-of-bcarr-box-in-the-amino-acid-repressed-genes-of-lactobacillus-helveticus-cm4
#10
Naoyuki Yamamoto, Taketo Wakai
BACKGROUND: A BCARR (branched-chain amino acid responsive repressor) identified in proteolytic gene expressions in Lactobacillus helveticus is considered to negatively control transcriptions by binding to operator sites at the promoter regions in the presence of BCAAs. However, the distributions and regulatory potential of the BCARR in all genes repressed by BCAAs in CM4 remains unclear. RESULTS: A genome-wide search for the BCARR-box was conducted to clarify the contribution of BCARR in the regulation of amino acid metabolism in L...
December 2, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/29196239/insulin-and-branched-chain-amino-acid-depletion-during-mouse-preimplantation-embryo-culture-programmes-body-weight-gain-and-raised-blood-pressure-during-early-postnatal-life
#11
Miguel A Velazquez, Bhavwanti Sheth, Stephanie J Smith, Judith J Eckert, Clive Osmond, Tom P Fleming
Mouse maternal low protein diet exclusively during preimplantation development (Emb-LPD) is sufficient to programme altered growth and cardiovascular dysfunction in offspring. Here, we use an in vitro model comprising preimplantation culture in medium depleted in insulin and branched-chain amino acids (BCAA), two proposed embryo programming inductive factors from Emb-LPD studies, to examine the consequences for blastocyst organisation and, after embryo transfer (ET), postnatal disease origin. Two-cell embryos were cultured to blastocyst stage in defined KSOM medium supplemented with four combinations of insulin and BCAA concentrations...
November 28, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29183656/unbiased-plasma-metabolomics-reveal-the-correlation-of-metabolic-pathways-and-prakritis-of-humans
#12
Amey Shirolkar, Sutapa Chakraborty, Tusharkanti Mandal, Rajesh Dabur
BACKGROUND: Ayurveda, an ancient Indian medicinal system, has categorized human body constitutions in three broad constitutional types (prakritis) i.e. Vata, Pitta and Kapha. OBJECTIVE: Analysis of plasma metabolites and related pathways to classify Prakriti specific dominant marker metabolites and metabolic pathways. MATERIALS AND METHODS: 38 healthy male individuals were assessed for dominant Prakritis and their fasting blood samples were collected...
November 25, 2017: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/29179484/association-of-circulating-branched-chain-amino-acids-with-cardiometabolic-traits-differs-between-adults-and-the-oldest-old
#13
Liang Sun, Caiyou Hu, Ruiyue Yang, Yuan Lv, Huiping Yuan, Qinghua Liang, Benjin He, Guofang Pang, Menghua Jiang, Jun Dong, Ze Yang
Branched-chain amino acids (BCAAs) are promising for their potential anti-aging effects. However, findings in adults suggest that circulating BCAAs are associated with cardiometabolic risk. Moreover, little information is available about how BCAAs influence clustered cardiometabolic traits in the oldest-old (>85 years), which are the fastest-growing segment of the population in developed countries. Here, we applied a targeted metabolomics approach to measure serum BCAAs in Chinese participants (aged 21-110 years) based on a longevity cohort...
October 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/29177054/increase-in-branched-chain-amino-acids-due-to-acidemia-in-neonatal-calves-with-diarrhoea
#14
Kenji Tsukano, Hiroki Inoue, Kazuyuki Suzuki
The aim of this study was to investigate the relationships between acid-base status and plasma branched-chain amino acids (BCAA) concentration in calves with diarrhoea for intravenous nutrition, especially with amino acid solution in calves with diarrhoea. Thirty-four Holstein calves aged 11.0±5.9 days old were enrolled in this study. In 10 of 34 calves exhibiting clinical signs of diarrhoea, severe dehydration and acidemia were observed (severe group: pH: 7.04±0.11, base excess (BE): -17.4±4.5) based on blood gas analysis...
2017: Veterinary Record Open
https://www.readbyqxmd.com/read/29157816/effect-of-ipomoea-aquatica-ethanolic-extract-in-streptozotocin-stz-induced-diabetic-rats-via-1-h-nmr-based-metabolomics-approach
#15
Azliana Abu Bakar Sajak, Ahmed Mediani, Maulidiani, Nur Sumirah Mohd Dom, Chandradevan Machap, Muhajir Hamid, Amin Ismail, Alfi Khatib, Faridah Abas
BACKGROUND: Ipomoea aquatica (locally known as "kangkung") has previously been reported to have hypoglycemic activities on glucose level in diabetes patients. However, the effect of I. aquatica ethanolic extract on the metabolites in the body has remained unknown. PURPOSE: This study provides new insights on the changes of endogenous metabolites caused by I. aquatica ethanolic extract and improves the understanding on the therapeutic efficacy and mechanism of I...
December 1, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29141216/slc3a2-mediates-branched-chain-amino-acid-dependent-maintenance-of-regulatory-t-cells
#16
Kayo Ikeda, Makoto Kinoshita, Hisako Kayama, Shushi Nagamori, Pornparn Kongpracha, Eiji Umemoto, Ryu Okumura, Takashi Kurakawa, Mari Murakami, Norihisa Mikami, Yasunori Shintani, Satoko Ueno, Ayatoshi Andou, Morihiro Ito, Hideki Tsumura, Koji Yasutomo, Keiichi Ozono, Seiji Takashima, Shimon Sakaguchi, Yoshikatsu Kanai, Kiyoshi Takeda
Foxp3(+) regulatory T (Treg) cells, which suppress immune responses, are highly proliferative in vivo. However, it remains unclear how the active replication of Treg cells is maintained in vivo. Here, we show that branched-chain amino acids (BCAAs), including isoleucine, are required for maintenance of the proliferative state of Treg cells via the amino acid transporter Slc3a2-dependent metabolic reprogramming. Mice fed BCAA-reduced diets showed decreased numbers of Foxp3(+) Treg cells with defective in vivo proliferative capacity...
November 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/29122443/identification-of-serum-metabolites-associated-with-obesity-and-traditional-risk-factors-for-metabolic-disease-in-chinese-adults
#17
S M Wang, R Y Yang, M Wang, F S Ji, H X Li, Y M Tang, W X Chen, J Dong
BACKGROUND AND AIMS: Obesity is a major worldwide health problem and is often associated with many metabolic diseases. Levels of several serum-specific metabolites may be altered in patients with these metabolic diseases. We aimed to investigate the associations of serum metabolite levels with obesity and traditional risk factors for metabolic disease in Chinese individuals. METHODS AND RESULTS: Six-hundred Chinese individuals undergoing annual physical exams were recruited and categorized into overweight/obese and control groups (1:1 ratio)...
October 3, 2017: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/29118722/branched-chain-amino-acid-negatively-regulates-klf15-expression-via-pi3k-akt-pathway
#18
Yunxia Liu, Weibing Dong, Jing Shao, Yibin Wang, Meiyi Zhou, Haipeng Sun
Recent studies have linked branched-chain amino acid (BCAA) with numerous metabolic diseases. However, the molecular basis of BCAA's roles in metabolic regulation remains to be established. KLF15 (Krüppel-like factor 15) is a transcription factor and master regulator of glycemic, lipid, and amino acids metabolism. In the present study, we found high concentrations of BCAA suppressed KLF15 expression while BCAA starvation induced KLF15 expression, suggesting KLF15 expression is negatively controlled by BCAA...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29104034/distribution-of-the-branched-chain-%C3%AE-ketoacid-dehydrogenase-complex-e1%C3%AE-subunit-and-glutamate-dehydrogenase-in-the-human-brain-and-their-role-in-neuro-metabolism
#19
Jonathon Hull, Marcela Usmari Moraes, Emma Brookes, Seth Love, Myra E Conway
Glutamate is the major excitatory neurotransmitter of the central nervous system, with the branched-chain amino acids (BCAAs) acting as key nitrogen donors for de novo glutamate synthesis. Despite the importance of these major metabolites, their metabolic pathway in the human brain is still not well characterised. The metabolic pathways that influence the metabolism of BCAAs have been well characterised in rat models. However, the expression of key proteins such as the branched-chain α-ketoacid dehydrogenase (BCKD) complex and glutamate dehydrogenase isozymes (GDH) in the human brain is still not well characterised...
November 9, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/29075976/specific-plasma-amino-acid-disturbances-associated-with-metabolic-syndrome
#20
Marta Siomkajło, Jacek Rybka, Magdalena Mierzchała-Pasierb, Andrzej Gamian, Joanna Stankiewicz-Olczyk, Marek Bolanowski, Jacek Daroszewski
PURPOSE: The primary objective of the present study was to examine the association between branched chain and aromatic amino acid profiles (BCAA and AAA respectively) and the metabolic syndrome (MS), and to evaluate the clinical utility of these associations in the diagnostic process. METHODS: Two hundred and sixty three healthy men with MS [MS(+): n = 165] and without MS [MS(-): n = 98] were enrolled in the observational study. Anthropometrical, biochemical, and amino acid measurements were performed...
October 26, 2017: Endocrine
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