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https://www.readbyqxmd.com/read/29350259/diminished-expression-of-%C3%AE-2-gpi-is-associated-with-a-reduced-ability-to-mitigate-complement-activation-in-anti-gpiib-iiia-mediated-immune-thrombocytopenia
#1
Xiaolu Zhu, Jiamin Zhang, Qianming Wang, Haixia Fu, Yingjun Chang, Yuan Kong, Meng Lv, Lanping Xu, Kaiyan Liu, Xiaojun Huang, Xiaohui Zhang
Anti-GPIIb/IIIa-mediated complement activation has been reported to be important in the pathogenesis of immune thrombocytopenia (ITP). However, the role of the complement system and the involved regulatory mechanism remain equivocal. Beta2-glycoprotein I (β2-GPI), known as the main target for antiphospholipid autoantibodies, has been demonstrated as a complement regulator. Here, we investigated the complement-regulatory role of β2-GPI in anti-GPIIb/IIIa-mediated ITP. Plasma complement activation and enhanced complement activation capacity (CAC) were found in ITP patients with anti-GPIIb/IIIa antibodies in vivo and in vitro...
December 29, 2017: Annals of Hematology
https://www.readbyqxmd.com/read/29349668/serum-mmp-9-diagnostics-prognostics-and-activation-in-acute-coronary-syndrome-and-its-recurrence
#2
Laura Lahdentausta, Jaakko Leskelä, Alina Winkelmann, Taina Tervahartiala, Timo Sorsa, Erkki Pesonen, Pirkko J Pussinen
Matrix metalloproteinase (MMP)-9 is crucial in atherosclerotic plaque rupture and tissue remodeling after a cardiac event. The balance between MMP-9 and endogenous inhibitor, tissue inhibitors of matrix metalloproteinase 1 (TIMP-1), is important in acute coronary syndrome (ACS). This is an age- and gender-matched case-control study of ACS (N = 669). Patients (45.7%) were resampled after recovery, and all were followed up for 6 years. The molecular forms of MMP-9 were investigated by gelatin zymography...
January 18, 2018: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/29349660/progestogen-hypersensitivity
#3
REVIEW
Rung-Chi Li, Kathleen M Buchheit, Jonathan A Bernstein
PURPOSE OF REVIEW: Progestogen hypersensitivity (PH) is a rare disorder which usually occurs in women of childbearing age with symptoms ranging from urticaria with or without angioedema, multiple organ involvement consistent with allergic anaphylaxis, to a spectrum of other non-evanescent skin eruptions. In this review, we present a clinical vignette of PH and discuss the clinical presentation and proposed pathomechanisms, diagnosis, and treatment of PH. RECENT FINDINGS: The hypersensitivity symptoms are associated with exogenous progestin exposure (e...
January 19, 2018: Current Allergy and Asthma Reports
https://www.readbyqxmd.com/read/29349524/-qualification-and-classification-of-medical-apps-what-should-be-noted-and-what-is-bfarm-s-contribution
#4
REVIEW
Nicole Rämsch-Günther, Sylvia Stern, Wolfgang Lauer
Smartphones and tablets with their nearly unlimited number of different applications have become an integral part of everyday life. Thus, mobile devices and applications have also found their way into the healthcare sector.For developers, manufacturers, or users as well, it is often difficult to decide whether a mobile health application is a medical device.In this context, it is extremely important for manufacturers to decide at an early stage of the development whether the product is to be introduced into the market as a medical device and is therefore subject to the legislation on medical devices...
January 18, 2018: Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
https://www.readbyqxmd.com/read/29348845/decreased-expression-of-the-long-non-coding-rna-slc7a11-as1-predicts-poor-prognosis-and-promotes-tumor-growth-in-gastric-cancer
#5
Yajun Luo, Cheng Wang, Peng Yong, Pengcheng Ye, Zilin Liu, Zhiming Fu, Fei Lu, Wanping Xiang, Wang Tan, Jiangwei Xiao
Many lncRNA and mRNA sense-antisense transcripts have been systematically identified in malignant cells. However, the molecular mechanisms of most lncRNA-mRNA pairs in gastric cancer remain largely unknown. We found the gastric cancer-associated lncRNA SLC7A11-AS1 and coding transcript mRNA SLC7A11 in human gastric cancer specimens by microarray. SLC7A11-AS1, antisense to SLC7A11, is significantly down-regulated in gastric cancer and could promote tumor growth in vitro and in vivo. The effects of SLC7A11-AS1 depend on the regulation of SLC7A11 via the ASK1-p38MAPK/JNK signaling pathway...
December 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29345429/engineering-3d-hydrogels-for-personalized-in-vitro-human-tissue-models
#6
REVIEW
Chya-Yan Liaw, Shen Ji, Murat Guvendiren
There is a growing interest in engineering hydrogels for 3D tissue and disease models. The major motivation is to better mimic the physiological microenvironment of the disease and human condition. 3D tissue models derived from patients' own cells can potentially revolutionize the way treatment and diagnostic alternatives are developed. This requires development of tissue mimetic hydrogels with user defined and tunable properties. In this review article, a recent summary of 3D hydrogel platforms for in vitro tissue and disease modeling is given...
January 18, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29344289/limb-bud-and-heart-overexpression-inhibits-the-proliferation-and-migration-of-pc3m-cells
#7
Qicai Liu, Ermao Li, Long Huang, Minsheng Cheng, Li Li
Background: The limb-bud and heart gene (LBH) was discovered in the early 21st century and is specifically expressed in the mouse embryonic limb and heart development. Increasing evidences have indicated that LBH not only plays an important role in embryo development, it is also closely correlated with the occurance and progression of many tumors. However, its function in prostate cancer (PCa) is still not well understood. Here, we explored the effects of LBH on the proliferation and migration of the PCa cell line PC3M...
2018: Journal of Cancer
https://www.readbyqxmd.com/read/29341939/the-quest-for-equivalence-of-test-results-the-pilgrimage-of-the-dutch-calibration-2-000-program-for-metrological-traceability
#8
REVIEW
Rob T P Jansen, Christa M Cobbaert, Cas Weykamp, Marc Thelen
Calibration 2.000 was initiated 20 years ago for standardization and harmonization of medical tests. The program also intended to evaluate adequate implementation of the In Vitro Diagnostics (IVD) 98/79/EC directive, in order to ensure that medical tests are fit-for-clinical purpose. The Calibration 2.000 initiative led to ongoing verification of test standardization and harmonization in the Netherlands using commutable external quality assessment (EQA)-tools and a type 1 EQA-design, where feasible. National support was guaranteed by involving all laboratory professionals as well as laboratory technicians responsible for EQA and quality officers...
January 17, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29340086/up-regulation-of-lncrna-snhg1-indicates-poor-prognosis-and-promotes-cell-proliferation-and-metastasis-of-colorectal-cancer-by-activation-of-the-wnt-%C3%AE-catenin-signaling-pathway
#9
Yuping Zhu, Bo Li, Zhuo Liu, Lai Jiang, Gang Wang, Min Lv, Dechuan Li
Recently, the lncRNA small nucleolar RNA host gene (SNHG1) has been exhibited to be upregulated, which plays a crucial role in the development and prognosis of several cancers. However, the role of the biology and clinical significance of SNHG1 in the tumorigenesis of colorectal cancer (CRC) has rarely been reported. In this work, we firstly found that SNHG1 expression levels were upregulated aberrantly in colorectal cancer tissues and colorectal cancer cell lines. By Kaplan-Meier survival analysis, patients with high SNHG1 expression level had poorer overall survival (OS) and progression-free survival (PFS) than those with low SNHG1 expression...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29340052/gene-expression-profiling-and-construction-of-a-putative-gene-regulatory-network-of-bladder-cancer-tumor-initiating-cells
#10
Zhuoyuan Xin, Zhao Yang, Jianting Xu, Chaoying Li, Tong Shao, Guoqing Wang, Chong Li
Human bladder cancer tumors have been shown to contain a subpopulation of cells with stem-like characteristics that may trigger tumor growth, recurrence, and metastasis. These cells, known as tumor-initiating cells (TICs), would be effective diagnostic tools and valuable therapeutic targets. Here, we report the isolation of TICs from seven bladder cancer cell lines and show that TICs from different sources vary on their ability to form tumorspheres in vitro and generate xenografts in vivo, which suggest they are remarkably heterogeneous...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29339127/the-basophil-activation-test-can-be-of-value-for-diagnosing-immediate-allergic-reactions-to%C3%A2-omeprazole
#11
Jose J Laguna, Gador Bogas, Maria Salas, Cristobalina Mayorga, Javier Dionicio, Rosario Gonzalez-Mendiola, Adriana Ariza, Ruben Fernández-Santamaría, Isabel Olazabal, Inmaculada Doña, Tahia D Fernandez, Maria J Torres
BACKGROUND: Proton pump inhibitors (PPIs) are commonly used to treat gastrointestinal diseases. Incidence of hypersensitivity reactions to PPIs has risen, likely because of increased consumption. Their diagnosis is difficult, with skin tests (STs) presenting low sensitivity, making it necessary to perform drug provocation tests (DPTs). The value of in vitro tests for the diagnosis of immediate reaction to PPI is unclear. OBJECTIVE: To analyze the diagnostic value of the basophil activation test (BAT) in a group of patients diagnosed with immediate allergy to omeprazole...
January 12, 2018: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/29336181/analytical-and-between-subject-variation-of-thrombin-generation-measured-by-calibrated-automated-thrombography-on-plasma-samples
#12
Anne F Kristensen, Søren R Kristensen, Ursula Falkmer, Anna-Marie B Münster, Shona Pedersen
BACKGROUND: The Calibrated Automated Thrombography (CAT) is an in vitro thrombin generation (TG) assay that holds promise as a valuable tool within clinical diagnostics. However, the technique has a considerable analytical variation, and we therefore, investigated the analytical and between-subject variation of CAT systematically. Moreover, we assess the application of an internal standard for normalization to diminish variation. METHODS: 20 healthy volunteers donated one blood sample which was subsequently centrifuged, aliquoted and stored at -80 °C prior to analysis...
January 16, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/29336142/a-smart-dual-quenching-strategy-enhances-the-detection-sensitivity-of-intracellular-furin
#13
Zijuan Hai, Jingjing Wu, Dilizhatai Saimi, Yanhan Ni, Rongbin Zhou, Gaolin Liang
Development of sensitive fluorescence "Turn-On" strategies for imaging enzyme activity in living cells is of disease-diagnostic importance but remains challenging. Herein, by employing a click condensation reaction and rational design of a single quenched probe Cys(StBu)-Lys(Gly-Lys(DABCYL)-Gly-Gly-Arg-Arg-Val-Arg-Gly-FITC)-CBT (1), we developed a "smart" dual quenching strategy and applied it to detect intracellular furin activity with enhanced sensitivity. At physiological condition, 1 was subjected to reduction-controlled condensation reaction to form 1-NPs and its fluorescence intensity further dropped to 1/2...
January 16, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29335340/anticitrullinated-protein-antibodies-induce-inflammatory-gene-expression-profile-in-peripheral-blood-cells-from-ccp-positive-patients-with-ra
#14
Smadar Gertel, Gidi Karmon, Eszter Szarka, Ora Shovman, Esther Houri-Levi, Edna Mozes, Yehuda Shoenfeld, Howard Amital
OBJECTIVE: Anticitrullinated protein antibodies (ACPA) have major diagnostic significance in rheumatoid arthritis (RA). ACPA are directed against different citrullinated antigens, including filaggrin, fibrinogen, vimentin, and collagen. The presence of ACPA is associated with joint damage and extraarticular manifestations, suggesting that ACPA may have a significant role in the pathogenesis of RA. METHODS: To verify the effect of ACPA on RA-immune cells, peripheral blood mononuclear cells (PBMC) from cyclic citrullinated peptide (CCP)-positive patients with RA and healthy controls were cocultured in vitro with ACPA...
January 15, 2018: Journal of Rheumatology
https://www.readbyqxmd.com/read/29333615/circular-rna-hsa_circ_0000567-can-be-used-as-a-promising-diagnostic-biomarker-for-human-colorectal-cancer
#15
Jianjun Wang, Xiaomin Li, Linming Lu, Lei He, Haoran Hu, Zengxiang Xu
BACKGROUND: Recent studies have revealed that circular RNAs are involved in the biological process of some kinds of human cancers. However, little is known about their diagnostic values and functions in colorectal cancer (CRC). METHODS: The expression levels of hsa_circ_0000567 in 102 paired CRC tissues and adjacent noncancerous tissues, 5 CRC cell lines, and a normal colorectal epithelial cell line were detected by quantitative real-time polymerase chain reaction (qRT-PCR)...
January 15, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29332593/facile-synthesis-crystal-structure-dft-calculation-and-biological-activities-of-4-2-fluorophenyl-3-3-methoxybenzyl-1h-1-2-4-triazol-5-4h-one-5
#16
Muhammad Saleem, Muhammad Rafiq, Yeon Ki Jeong, Dae Won Cho, Chong-Hyeak Kim, Sung-Yum Seo, Chang-Shik Choi, Seong-Karp Hong, Ki-Hwan Lee
BACKGROUND: In the past few decades, the design, synthesis and characterization of novel heterocyclic compounds with auspicious biological profile received the considerable attention of scientific community. Among them, the small and simple organic molecular backbone like triazole moiety have broad spectrum of applications in the medicinal as well as diagnostic areas. OBJECTIVE: The objective of present study was the synthesis, characterization and exploration of biological profile of 4-(2-fluorophenyl)-3-(3-methoxybenzyl)-1H-1,2,4-triazol-5(4H)-one (5)...
January 12, 2018: Medicinal Chemistry
https://www.readbyqxmd.com/read/29331015/detection-of-human-disease-conditions-by-single-cell-morpho-rheological-phenotyping-of-blood
#17
Nicole Toepfner, Christoph Herold, Oliver Otto, Philipp Rosendahl, Angela Jacobi, Martin Kräter, Julia Stächele, Leonard Menschner, Maik Herbig, Laura Ciuffreda, Lisa Ranford-Cartwright, Michal Grzybek, Ünal Coskun, Elisabeth Reithuber, Genevieve Garriss, Peter Mellroth, Birgitta Henriques Normark, Nicola Tregay, Meinolf Suttorp, Martin Bornhäuser, Edwin R Chilvers, Reinhard Berner, Jochen Guck
Blood is arguably the most important bodily fluid and its analysis provides crucial health status information. A first routine measure to narrow down diagnosis in clinical practice is the differential blood count, determining the frequency of all major blood cells. What is lacking to advance initial blood diagnostics is an unbiased and quick functional assessment of blood that can narrow down the diagnosis and generate specific hypotheses. To address this need, we introduce the continuous, cell-by-cell morpho-rheological (MORE) analysis of diluted whole blood, without labeling, enrichment or separation, at rates of 1,000 cells/sec...
January 13, 2018: ELife
https://www.readbyqxmd.com/read/29330435/beta-catenin-cleavage-enhances-transcriptional-activation
#18
Tatiana Goretsky, Emily M Bradford, Qing Ye, Olivia F Lamping, Tomas Vanagunas, Mary Pat Moyer, Patrick C Keller, Preetika Sinh, Josep M Llovet, Tianyan Gao, Qing-Bai She, Linheng Li, Terrence A Barrett
Nuclear activation of Wnt/β-catenin signaling is required for cell proliferation in inflammation and cancer. Studies from our group indicate that β-catenin activation in colitis and colorectal cancer (CRC) correlates with increased nuclear levels of β-catenin phosphorylated at serine 552 (pβ-Cat552). Biochemical analysis of nuclear extracts from cancer biopsies revealed the existence of low molecular weight (LMW) pβ-Cat552, increased to the exclusion of full size (FS) forms of β-catenin. LMW β-catenin lacks both termini, leaving residues in the armadillo repeat intact...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330297/characterization-and-evidence-of-the-mir-888-cluster-as-a-novel-cancer-network-in-prostate
#19
Tsuyoshi Hasegawa, Garrison Glavich, Mary Pahuski, Aleena M Short, Oliver John Semmes, Lifang Yang, Vitold Galkin, Richard R Drake, Aurora Esquela-Kerscher
Prostate cancer afflicts 1 in 7 men and is the second leading cause of male cancer-related deaths in the United States. MicroRNAs (miRNAs), an extensive class of ~22 nucleotide non-coding RNAs, are often aberrantly expressed in tissues and fluids from prostate cancer patients but the mechanism of how specific miRNAs regulate prostate tumorigenesis and metastasis are poorly understood. Here, miR-888 was identified as a novel prostate factor that promotes proliferation and migration. miR-888 resides within a genomic cluster of 7 miRNA genes (mir-892c, mir-890, mir-888, mir-892a, mir-892b, mir-891b, mir-891a) on human chromosome Xq27...
January 12, 2018: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29330128/exploiting-ion-channel-structure-to-assess-rare-variant-pathogenicity
#20
Brett M Kroncke, Tao Yang, Prince Kannankeril, M Benjamin Shoemaker, Dan M Roden
BACKGROUND: A 27-year-old female was seen for long QT syndrome. She was found to be a carrier of two variants, KCNQ1 Val162Met and KCNH2 Ser55Leu, and both were annotated as pathogenic by a diagnostic laboratory in part because of sequence proximity to other known pathogenic variants. OBJECTIVE: To assess the relationship between both the KCNQ1 and KCNH2 variants and clinical significance using protein structure, in vitro functional assays, and familial segregation...
January 9, 2018: Heart Rhythm: the Official Journal of the Heart Rhythm Society
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