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https://www.readbyqxmd.com/read/28636767/aberrant-expression-of-kallikrein-related-peptidase-7-is-correlated-to-human-melanoma-aggressiveness-by-stimulating-cell-migration-and-invasion
#1
Tiphaine Delaunay, Lydia Deschamps, Meriem Haddada, Francine Walker, Antoninus Soosaipillai, Feryel Soualmia, Chahrazade El Amri, Eleftherios P Diamandis, Maria Brattsand, Viktor Magdolen, Dalila Darmoul
Members of the tissue kallikrein-related peptidase (KLK) family not only regulate several important physiological functions, but aberrant expression has also been associated with various malignancies. Clinically, KLKs have been suggested as promising biomarkers for diagnosis and prognosis in many types of cancer. As of yet, expression of KLKs and their role in skin cancers are, however, poorly addressed. Malignant melanoma is an aggressive disease associated with poor prognosis. Hence, diagnostic biomarkers to monitor melanoma progression are needed...
June 21, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/28635419/multimodal-imaging-gd-nanoparticles-functionalized-with-pittsburgh-compound-b-or-a-nanobody-for-amyloid-plaques-targeting
#2
Jonathan Pansieri, Marie Plissonneau, Nathalie Stransky-Heilkron, Mireille Dumoulin, Laurence Heinrich-Balard, Pascaline Rivory, Jean-François Morfin, Eva Toth, Maria Joao Saraiva, Eric Allémann, Olivier Tillement, Vincent Forge, François Lux, Christel Marquette
AIM: Gadolinium-based nanoparticles were functionalized with either the Pittsburgh compound B or a nanobody (B10AP) in order to create multimodal tools for an early diagnosis of amyloidoses. MATERIALS & METHODS: The ability of the functionalized nanoparticles to target amyloid fibrils made of β-amyloid peptide, amylin or Val30Met-mutated transthyretin formed in vitro or from pathological tissues was investigated by a range of spectroscopic and biophysics techniques including fluorescence microscopy...
June 21, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28635252/proton-fueled-reversible-dna-hybridization-chain-assembly-for-ph-sensing-and-imaging
#3
Lan Liu, Jin-Wen Liu, Zhi-Mei Huang, Han Wu, Na Li, Li-Juan Tang, Jian-Hui Jiang
Design of DNA self-assembly with reversible responsiveness to external stimuli is of great interest for diverse applications. We for the first time develop a pHresponsive, fully-reversible hybridization chain reaction (HCR) assembly that allows sensitive sensing and imaging of pH in living cells. Our design relies on the triplex forming sequences that form DNA triplex with toehold regions under acidic conditions and then induce a cascade of strand displacement and DNA assembly. The HCR assembly has shown dynamic responses in physiological pH ranges with excellent reversibility and demonstrated the potential for in vitro detection and live-cell imaging of pH...
June 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28630300/g9a-drives-hypoxia-mediated-gene-repression-for-breast-cancer-cell-survival-and-tumorigenesis
#4
Francesco Casciello, Fares Al-Ejeh, Greg Kelly, Donal J Brennan, Shin Foong Ngiow, Arabella Young, Thomas Stoll, Karolina Windloch, Michelle M Hill, Mark J Smyth, Frank Gannon, Jason S Lee
G9a is an epigenetic regulator that methylates H3K9, generally causing repression of gene expression, and participates in diverse cellular functions. G9a is genetically deregulated in a variety of tumor types and can silence tumor suppressor genes and, therefore, is important for carcinogenesis. Although hypoxia is recognized to be an adverse factor in tumor growth and metastasis, the role of G9a in regulating gene expression in hypoxia has not been described extensively. Here, we show that G9a protein stability is increased in hypoxia via reduced proline hydroxylation and, hence, inefficient degradation by the proteasome...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28629464/mir-629-3p-may-serve-as-a-novel-biomarker-and-potential-therapeutic-target-for-lung-metastases-of-triple-negative-breast-cancer
#5
Jin Wang, Cailu Song, Hailin Tang, Chao Zhang, Jun Tang, Xing Li, Bo Chen, Xiaoming Xie
BACKGROUND: Different breast cancer subtypes show distinct tropisms for sites of metastasis. Notably, the lung is the most common site for the first distant recurrence in triple-negative breast cancer (TNBC). The identification of novel biomarkers for lung metastasis is of great importance to improving the outcome of TNBC. In this study, we sought to identify a microRNA (miRNA)-based biomarker and therapeutic target for lung metastasis of TNBC. METHODS: A total of 669 patients without de novo stage IV TNBC were recruited for this study...
June 19, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28629059/versatile-quarto-stimuli-nanostructure-based-on-trojan-horse-approach-for-cancer-therapy-synthesis-characterization-in-vitro-and-in-vivo-studies
#6
Eleni K Efthimiadou, Eirini Fragogeorgi, Lazaros Palamaris, Theodoros Karampelas, Pavlos Lelovas, George Loudos, Constantin Tamvakopoulos, Nikolaos Kostomitsopoulos, George Kordas
Nanostructured delivery and diagnostic systems that induces specific targeting properties by exploiting the local physicochemical tumour characteristics will be evaluated is the present work. It is well known that cancer cells have specific physicochemical characteristics, which can be taken into consideration for the design of a broad spectrum of drug delivery systems (DDS). Some of those characteristics including the different temperature environment their susceptibility when temperature ranges between 40 and 43°C where cell apoptosis is induced, the intra- and extra-cellular pH which varies from 6...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28628008/basophil-histamine-release-to-amoxicilloyl-poly-l-lysine-compared-to-amoxicillin-in-patients-with-ige-mediated-allergic-reactions-to-amoxicillin
#7
F Arribas, S Falkencrone, J Sola, M P Gomez-Serranillos, J J Laguna, M I Montañez, T D Fernandez, D Rodríguez, F Pineda, P Stahl Skov, C Mayorga, M J Torres
BACKGROUND: Amoxicillin (AX) is the betalactam most often involved in IgE-mediated reactions and the diagnosis is mainly based on skin testing (ST) although without optimal sensitivity. We have produced a newly AX derivative, amoxicilloyl-poly-L-lysine (APL), and have analysed its IgE recognition by passive histamine release test (pHRT). METHODS: Patients (N=19) with confirmed AX allergy and with specific IgE positive to AX and controls (N=10) with good tolerance to AX were included...
June 19, 2017: Journal of Investigational Allergology & Clinical Immunology
https://www.readbyqxmd.com/read/28627667/loss-of-mir-449a-caused-prlz-overexpression-promotes-prostate-cancer-metastasis
#8
Wei Chen, Yi Liu, Hongde Chen, Hao Ning, Kejia Ding
Distant metastasis is the worst prognostic factor for PCa patients. It has been reported that miR-449a enhances radiosensitivity of prostate cancer cells, but the function of miR449a in metastasis of prostate cancer is mainly unknown. In the present study, we strove to investigate the function and diagnostic value of miR-449a in metastasis of prostate cancer. qRT-PCR was used to quantify the expression of miR449a and PrLZ in PCa cell lines and tissues. we found that miR449a expression was decreased in PCa cell lines...
June 12, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28624617/core-shell-iron-oxide-layered-double-hydroxide-high-electrochemical-sensing-performance-of-h2o2-biomarker-in-live-cancer-cells-with-plasma-therapeutics
#9
Muhammad Asif, Hongwei Liu, Ayesha Aziz, Haitao Wang, Zhengyun Wang, Muhammad Ajmal, Fei Xiao, Hongfang Liu
In this work, we develop a new type of multifunctional core-shell nanomaterial by controllable integration of CuAl layered double hydroxides (LDHs) over the surface of iron oxides (Fe3O4) nanospheres (NSs) to fabricate (Fe3O4@CuAl NSs) hybrid material with interior tunability of LDH phase and explore its practical application in ultrasensitive detection of emerging biomarker, i.e., H2O2 as cancer diagnostic probe. In addition, atmospheric pressure plasmas (APPs) have also been used as potential therapeutic approach for cancer treatment...
June 3, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28623535/nanotechnologies-for-in-vitro-ige-testing
#10
REVIEW
Iwan Märki, Fabien Rebeaud
PURPOSE OF REVIEW: This review discusses the recent advances in the development of IgE antibody assays based on nanotechnologies. IgE blood testing is an important part of the diagnostic workup of IgE-mediated hypersentivity. We also address the challenges in moving from an academic proof-of-concept to a product routinely used by allergy experts. RECENT FINDINGS: Several nanotechnologies have been applied to the field of IgE testing: nanoparticles are used either as a support to capture analytes or as a detection tool to enhance the measurement signal...
July 2017: Current Allergy and Asthma Reports
https://www.readbyqxmd.com/read/28619954/a-novel-immune-biomarker-ifi27-discriminates-between-influenza-and-bacteria-in-patients-with-suspected-respiratory-infection
#11
Benjamin M Tang, Maryam Shojaei, Grant P Parnell, Stephen Huang, Marek Nalos, Sally Teoh, Kate O'Connor, Stephen Schibeci, Amy L Phu, Anand Kumar, John Ho, Adrienne F A Meyers, Yoav Keynan, Terry Ball, Amarnath Pisipati, Aseem Kumar, Elizabeth Moore, Damon Eisen, Kevin Lai, Mark Gillett, Robert Geffers, Hao Luo, Fahad Gul, Jens Schreiber, Sandra Riedel, David Booth, Anthony McLean, Klaus Schughart
Host response biomarkers can accurately distinguish between influenza and bacterial infection. However, published biomarkers require the measurement of many genes, thereby making it difficult to implement them in clinical practice. This study aims to identify a single-gene biomarker with a high diagnostic accuracy equivalent to multi-gene biomarkers.In this study, we combined an integrated genomic analysis of 1071 individuals with in vitro experiments using well-established infection models.We identified a single-gene biomarker, IFI27, which had a high prediction accuracy (91%) equivalent to that obtained by multi-gene biomarkers...
June 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28619525/murine-and-human-pancreatic-tumor-exosome-recovery-in-mouse-serum-diagnostic-and-prognostic-potential-and-target-cell-delivery
#12
Ulrike Erb, Kun Zhao, Zhe Wang, Li Xiao, Margot Zöller
Exosomes (Exo), powerful intercellular communicators, are recovered in all body fluids, suggesting suitability for diagnosis and prognosis. Easy in vitro manipulation recommends Exo as drug vehicles. Aiming to consolidate diagnostic and therapeutic potential of Exo, we evaluated recovery and fate of tumor (TEX) and exogenous Exo in syngeneic and xenogeneic mice bearing a murine or a human pancreatic adenocarcinoma. A significant increase in serum (S)-TEX was observed 2 weeks after tumor cell application. Instead, S-TEX declined within 3-6 days after tumor excision...
June 12, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28618993/new-perspectives-on-biomedical-applications-of-iron-oxide-nanoparticles
#13
Massimiliano Magro, Davide Baratella, Emanuela Bonaiuto, Jessica de Almeida Roger, Fabio Vianello
Iron oxide nanomaterials are considered promising tools for improved therapeutic efficacy and diagnostic applications in biomedicine. Accordingly, engineered iron oxide nanomaterials are increasingly proposed in biomedicine, and the interdisciplinary researches involving physics, chemistry, biology (nanotechnology) and medicine have led to exciting developments in the last decades. The progresses of the development of magnetic nanoparticles with tailored physico-chemical and surface properties produced a variety of clinically relevant applications, spanning from magnetic resonance imaging (MRI), drug delivery, magnetic hyperthermia, to in vitro diagnostics...
June 16, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28618003/a-multi-template-multiplex-pcr-assay-for-hepatitis-b-virus-and-human-%C3%AE-globin
#14
Oluwapelumi O Adeyemi, Morgan R Herod, Femi Oladiji, Yisa M Fakunle, Abiola S Babatunde, Olajide O Agbede
The Hepatitis B surface antigen (HBsAg) is the hallmark of HBV infection. Detection of antibodies to HBs and the core (i.e. HBsAg and HBcAb) are primary serological algorithms in the laboratory diagnosis of HBV. Detection of HBsAg DNA is an important supplement to serological diagnosis especially in clinical cases. Simultaneous amplification of internal cellular controls is a good indicator of sample quality. Human β-globin is a well characterised housekeeping gene (HKG) that is often applied as internal controls (IC) in molecular diagnosis...
June 15, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28617334/the-molecular-effect-of-diagnostic-absorbed-doses-from-131-i-on-papillary-thyroid-cancer-cells-in-vitro
#15
Mariusz Stasiołek, Zbigniew Adamczewski, Przemysław W Śliwka, Bartosz Puła, Bolesław Karwowski, Anna Merecz-Sadowska, Marek Dedecjus, Andrzej Lewiński
Diagnostic whole-body scan is a standard procedure in patients with thyroid cancer prior to the application of a therapeutic dose of (131)I. Unfortunately, administration of the radioisotope in a diagnostic dose may decrease further radioiodine uptake-the phenomenon called "thyroid stunning". We estimated radiation absorbed dose-dependent changes in genetic material, in particular in the sodium iodide symporter (NIS) gene promoter, and the NIS protein level in a K1 cell line derived from the metastasis of a human papillary thyroid carcinoma exposed to (131)I in culture...
June 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28616954/surface-charge-and-particle-size-determine-the-metabolic-fate-of-dendritic-polyglycerols
#16
Kritee Pant, Johanna Pufe, Kristof Zarschler, Ralf Bergmann, Jörg Steinbach, Sabine Reimann, Rainer Haag, Jens Pietzsch, Holger Stephan
Dendritic polyglycerols (dPG) are water soluble, polyether-based nanomaterials which hold great potential in diagnostic as well as therapeutic applications. In order to translate them for in vivo applications, a systematic assessment regarding their cell and tissue interactions as well as their metabolic fate in vivo is a crucial step. Herein, we explore the structure-activity relationship of three different sizes (ca. 3, 5, and 10 nm) of neutral dendritic polyglycerol (dPG) and their corresponding negatively charged sulfate analogs (dPGS) on their in vitro and in vivo characteristics...
June 15, 2017: Nanoscale
https://www.readbyqxmd.com/read/28615690/cathepsin-d-regulates-lipid-metabolism-in-murine-steatohepatitis
#17
Tom Houben, Yvonne Oligschlaeger, Tim Hendrikx, Albert V Bitorina, Sofie M A Walenbergh, Patrick J van Gorp, Marion J J Gijbels, Silvia Friedrichs, Jogchum Plat, Frank G Schaap, Dieter Lütjohann, Marten H Hofker, Ronit Shiri-Sverdlov
Due to the obesity epidemic, non-alcoholic steatohepatitis (NASH) is a prevalent liver disease, characterized by fat accumulation and inflammation of the liver. However, due to a lack of mechanistic insight, diagnostic and therapeutic options for NASH are poor. Recent evidence has indicated cathepsin D (CTSD), a lysosomal enzyme, as a marker for NASH. Here, we investigated the function of CTSD in NASH by using an in vivo and in vitro model. In addition to diminished hepatic inflammation, inhibition of CTSD activity dramatically improved lipid metabolism, as demonstrated by decreased plasma and liver levels of both cholesterol and triglycerides...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28615034/novel-methodologies-and-technologies-to-assess-mid-palatal-suture-maturation-a-systematic-review
#18
Darren Isfeld, Manuel Lagravere, Vladimir Leon-Salazar, Carlos Flores-Mir
INTRODUCTION: A reliable method to assess midpalatal suture maturation to drive clinical decision-making, towards non-surgical or surgical expansion, in adolescent and young adult patients is needed. The objectives were to systematically review and evaluate what is known regarding contemporary methodologies capable of assessing midpalatal suture maturation in humans. METHODS: A computerized database search was conducted using Medline, PubMed, Embase and Scopus to search the literature up until October 5, 2016...
June 14, 2017: Head & Face Medicine
https://www.readbyqxmd.com/read/28612309/pre-implantation-genetic-diagnosis-should-we-use-icsi-for-all
#19
Baruch Feldman, Adva Aizer, Masha Brengauz, Keren Dotan, Jacob Levron, Eyal Schiff, Raoul Orvieto
OBJECTIVE: Intracytoplasmic sperm injection (ICSI) is commonly used during pre-implantation genetic diagnosis (PGD) in vitro fertilization (IVF), aiming to eliminate the risk of contamination from extraneous sperm DNA. Recently, ICSI "overuse" in non-male infertility has been doubted, since it does not offer an advantage over IVF. Prompted by the aforementioned observations, we sought to assess the accuracy of IVF vs ICSI in PGD cases, as might be reflected by a difference in the prevalence of discarded embryos as a consequent of parental contamination...
June 13, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28611301/lncrna-svugp2-suppresses-progression-of-hepatocellular-carcinoma
#20
Jiangfeng Hu, Chenlin Song, Bensong Duan, Xiaoyan Zhang, Dongliang Li, Liang Zhu, Hengjun Gao
Numerous studies indicate that long noncoding RNAs (lncRNAs) are dysregulated in hepatocellular carcinoma (HCC) and might serve as potential diagnostic biomarkers and therapeutic targets of HCC. Therefore, it is interesting to globally identify the lncRNAs altered in HCC. In our study, we used microarray to profile the levels of lncRNAs and mRNAs in three pairs of HCC and their adjacent noncancerous samples. We found lncRNA-SVUGP2, which is a splice variant of the UGP2 gene, was down-regulated in HCC samples and correlates with a better prognosis in patients with HCC...
May 29, 2017: Oncotarget
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