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In vitro diagnostic

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https://www.readbyqxmd.com/read/29792094/regulatory-context-and-validation-of-assays-for-clinical-mass-spectrometry-proteomics-cmsp-methods
#1
Christophe Hirtz, Pauline Bros, Cato Brede, Pierre Lescuyer, Aleksandra M Maceski, Jerome Vialaret, Vincent Delatour, Sylvain Lehmann
Clinical mass spectrometry proteomics (cMSP) assays are being increasingly used in clinical laboratories for analyzing peptides and proteins. It has therefore become urgent to characterize and validate the methods available for liquid chromatography-tandem mass spectrometry (LC/MS-MS) targeted quantification of peptide and protein biomarkers in biological fluids in the context of in vitro diagnostics. LC-MS/MS for the detection of peptides and proteins is currently the main approach used in the field of cMSP...
May 23, 2018: Critical Reviews in Clinical Laboratory Sciences
https://www.readbyqxmd.com/read/29790580/preclinical-evaluation-of-potential-infection-imaging-probe-68-ga-ga-dota-k-a9-in-sterile-and-infectious-inflammation
#2
Karin M Nielsen, Nis P Jørgensen, Majbritt H Kyneb, Per Borghammer, Rikke L Meyer, Trine R Thomsen, Dirk Bender, Svend B Jensen, Ole L Nielsen, Aage K O Alstrup
The development of bacteria-specific infection radiotracers is of considerable interest to improve diagnostic accuracy and enabling therapy monitoring. The aim of this study was to determine if the previously reported radiolabelled 1,4,7,10-tetraazacyclododecane-N,N',N″,N‴-tetraacetic acid (DOTA) conjugated peptide, [68 Ga]Ga-DOTA-K-A9 could detect a staphylococcal infection in vivo, and distinguish it from aseptic inflammation. An optimised [68 Ga]Ga-DOTA-K-A9 synthesis omitting the use of acetone was developed, yielding 93% ± 0...
May 23, 2018: Journal of Labelled Compounds & Radiopharmaceuticals
https://www.readbyqxmd.com/read/29789716/xct-slc7a11-mediated-metabolic-reprogramming-promotes-non-small-cell-lung-cancer-progression
#3
Xiangming Ji, Jun Qian, S M Jamshedur Rahman, Peter J Siska, Yong Zou, Bradford K Harris, Megan D Hoeksema, Irina A Trenary, Chen Heidi, Rosana Eisenberg, Jeffrey C Rathmell, Jamey D Young, Pierre P Massion
Many tumors increase uptake and dependence on glucose, cystine or glutamine. These basic observations on cancer cell metabolism have opened multiple new diagnostic and therapeutic avenues in cancer research. Recent studies demonstrated that smoking could induce the expression of xCT (SLC7A11) in oral cancer cells, suggesting that overexpression of xCT may support lung tumor progression. We hypothesized that overexpression of xCT occurs in lung cancer cells to satisfy the metabolic requirements for growth and survival...
May 23, 2018: Oncogene
https://www.readbyqxmd.com/read/29788016/aedes-communis-reactivity-is-associated-with-bee-venom-hypersensitivity-an-in-vitro-and-in-vivo-study
#4
Enrico Scala, Lia Pirrotta, Carina G Uasuf, Gianni Mistrello, Stefano Amato, Emma Cristina Guerra, Maria Locanto, Giorgia Meneguzzi, Mauro Giani, Lorenzo Cecchi, Damiano Abeni, Riccardo Asero
Mosquito bite is usually followed by a local reaction, but severe or systemic reaction may, in rare cases, occur. Allergic reactions to Aedes communis (Ac) may be underestimated due to the lack of reliable diagnostic tools. In this multicenter study, 205 individuals reporting large local reactions to Ac were enrolled and studied for cutaneous or IgE reactivity to Ac, Blattella germanica, Penaeus monodon, and Dermatophagoides pteronyssinus. Extract and molecular IgE reactivity to bees, wasps, hornets, and yellow jacket venoms were also studied in 119 patients with a clinical history of adverse reaction to Hymenoptera...
May 22, 2018: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/29785229/the-circular-rna-hsa_circ_0001445-regulates-the-proliferation-and-migration-of-hepatocellular-carcinoma-and-may-serve-as-a-diagnostic-biomarker
#5
Xianwei Zhang, Hu Zhou, Wei Jing, Ping Luo, Shili Qiu, Xuefang Liu, Man Zhu, Chunzi Liang, Mingxia Yu, Jiancheng Tu
Circular RNAs (circRNA), a class of noncoding RNAs, have been found to be involved in various diseases. Here, the expression levels of the circRNA hsa_circ_0001445 in 73 pairs of hepatocellular carcinoma (HCC) and adjacent nontumor tissues were investigated by quantitative real-time polymerase chain reaction (qRT-PCR). Our data demonstrate that the hsa_circ_0001445 levels were significantly decreased in HCC tissues ( P < 0.001) and markedly associated with the number of tumor foci ( P = 0.014). Furthermore, in vitro approaches showed that overexpression of hsa_circ_0001445 promoted apoptosis and inhibited proliferation, migration, and invasion of HCC-derived cells, suggesting that hsa_circ_0001445 might be involved in the development of HCC...
2018: Disease Markers
https://www.readbyqxmd.com/read/29781739/preimplantation-genetic-diagnosis-as-a-strategy-to-prevent-having-a-child-born-with-an-heritable-eye-disease
#6
Claudia Yahalom, Michal Macarov, Galit Lazer-Derbeko, Gheona Altarescu, Tal Imbar, Jordana H Hyman, Talia Eldar-Geva, Anat Blumenfeld
BACKGROUND: In developed countries, genetically inherited eye diseases are responsible for a high percentage of childhood visual impairment. We aim to report our experience using preimplantation genetic diagnostics (PGD) in order to avoid transmitting a genetic form of eye disease associated with childhood visual impairment and ocular cancer. MATERIAL AND METHODS: Retrospective case series of women who underwent in vitro fertilization (IVF) and PGD due to a familial history of inherited eye disease and/or ocular cancer, in order to avoid having a child affected with the known familial disease...
May 21, 2018: Ophthalmic Genetics
https://www.readbyqxmd.com/read/29780247/impact-of-nanoparticles-on-neuron-biology-current-research-trends
#7
REVIEW
Firdos Alam Khan, Dana Almohazey, Munthar Alomari, Sarah Ameen Almofty
Nanoparticles have enormous applications in textiles, cosmetics, electronics, and pharmaceuticals. But due to their exceptional physical and chemical properties, particularly antimicrobial, anticancer, antibacterial, anti-inflammatory properties, nanoparticles have many potential applications in diagnosis as well as in the treatment of various diseases. Over the past few years, nanoparticles have been extensively used to investigate their response on the neuronal cells. These nanoparticles cause stem cells to differentiate into neuronal cells and promote neuronal cell survivability and neuronal cell growth and expansion...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29775052/double-photosystems-based-z-scheme-photoelectrochemical-sensing-mode-for-ultrasensitive-detection-of-disease-biomarker-accompanying-3d-dna-walker
#8
Shuzhen Lv, Kangyao Zhang, Yongyi Zeng, Dianping Tang
A new double photosystems-based 'Z-scheme' photoelectrochemical (PEC) sensing platform is designed for ultrasensitive detection of prostate-specific antigen (PSA) by coupling with three-dimensional (3D) DNA walker. Two photosystems consist of CdS quantum dots (photosystem I; PS I) and BiVO4 photoactive materials (photosystem II; PS II), whereas gold nanoparticles (AuNPs) photodeposited on high-active {010} facets of BiVO4 are used as the electron mediators to promote electron transfer from conduction band (CB) of PS II to valence band (VB) of PS I...
May 18, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29774498/microrna-1231-exerts-a-tumor-suppressor-role-through-regulating-the-egfr-pi3k-akt-axis-in-glioma
#9
Jiale Zhang, Jie Zhang, Wenjin Qiu, Jian Zhang, Yangyang Li, Enjun Kong, Ailin Lu, Jia Xu, Xiaoming Lu
PURPOSE: MicroRNAs (miRNAs) have been shown to be involved in the initiation and progression of glioma. However, the underlying molecular mechanisms are still unclear. METHODS: We performed microarray analysis to evaluate miRNA expression levels in 158 glioma tissue samples, and examined miR-1231 levels in glioma samples and healthy brain tissues using qRT-PCR. In vitro analyses were performed using miR-1231 mimics, inhibitors, and siRNA targeting EGFR. We used flow cytometry, CCK-8 assays, and colony formation assays to examine glioma proliferation and cell cycle analysis...
May 17, 2018: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/29773654/genetic-code-expansion-and-live-cell-imaging-reveal-that-thr308-phosphorylation-is-irreplaceable-and-sufficient-for-akt1-activity
#10
Nileeka Balasuriya, Maya T Kunkel, Xuguang Liu, Kyle K Biggar, Shawn S-C Li, Alexandra C Newton, Patrick O'Donoghue
The proto-oncogene Akt/protein kinase B (PKB) is a pivotal signal transducer for growth and survival. Growth factor stimulation leads to Akt phosphorylation at two regulatory sites (Thr308, Ser473), acutely activating Akt signaling. Delineating the exact role of each regulatory site is, however, technically challenging and has remained elusive. Here, we used genetic code expansion to produce site-specifically phosphorylated Akt1 in order to dissect the contribution of each regulatory site to Akt1 activity. We achieved recombinant production of full length Akt1 containing site-specific pThr and pSer residues for the first time...
May 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29768700/cell-and-tissue-based-proteome-profiling-and-dual-imaging-of-apoptosis-markers-with-probes-derived-from-venetoclax-and-idasanutlin
#11
Zhengqiu Li, Dongsheng Zhu, Haijun Guo, Yu Chang, Yun Ni, Lin Li, Piliang Hao, Yong Xu, Ke Ding
Venetoclax (ABT-199) and idasanutlin (RG7388) are efficient anticancer drugs targeting two essential apoptosis markers, Bcl2 and MDM2, respectively. Recent studies have shown that the combination of these two drugs leads to remarkable enhancement of anticancer efficacy, both in vitro and in vivo. In an attempt to understand the mechanism of this synergistic effect, competitive affinity-based proteome profiling coupled with bioimaging was employed to characterize their protein targets in the same cancer cell line and tumor tissue...
May 16, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29768466/core-non-coding-rnas-of-piscirickettsia-salmonis
#12
Cristopher Segovia, Raul Arias-Carrasco, Alejandro J Yañez, Vinicius Maracaja-Coutinho, Javier Santander
Piscirickettsia salmonis, a fastidious Gram-negative intracellular facultative bacterium, is the causative agent o Piscirickettsiosis. P. salmonis has broad host range with a nearly worldwide distribution, causing significant mortality. The molecular regulatory mechanisms of P. salmonis pathogenesis are relatively unknown, mainly due to its difficult in vitro culture and genomic differences between genogroups. Bacterial non-coding RNAs (ncRNAs) are important post-transcriptional regulators of bacterial physiology and virulence that are predominantly transcribed from intergenic regions (trans-acting) or antisense strand of open reading frames (cis-acting)...
2018: PloS One
https://www.readbyqxmd.com/read/29764796/predicting-the-reasons-of-customer-complaints-a-first-step-toward-anticipating-quality-issues-of-in-vitro-diagnostics-assays-with-machine-learning
#13
Stephane Aris-Brosou, James Kim, Li Li, Hui Liu
BACKGROUND: Vendors in the health care industry produce diagnostic systems that, through a secured connection, allow them to monitor performance almost in real time. However, challenges exist in analyzing and interpreting large volumes of noisy quality control (QC) data. As a result, some QC shifts may not be detected early enough by the vendor, but lead a customer to complain. OBJECTIVE: The aim of this study was to hypothesize that a more proactive response could be designed by utilizing the collected QC data more efficiently...
May 15, 2018: JMIR Medical Informatics
https://www.readbyqxmd.com/read/29763554/template-catalyzed-disulfide-conjugation-of-monoclonal-antibodies-using-a-natural-amino-acid-tag
#14
Jeremy D King, Yuelong Ma, Yi-Chui Kuo, Krzysztof P Bzymek, Leah H Goodstein, Kassondra Meyer, Roger E Moore, Desiree Crow, David Colcher, Gagandeep Singh, David A Horne, John C Williams
The high specificity and favorable pharmacological properties of monoclonal antibodies (mAbs) have prompted significant interest in re-engineering this class of molecules to add novel functionalities for enhanced therapeutic and diagnostic potential. Here, we used the high affinity, meditope-Fab interaction to template and drive the rapid, efficient, and stable site-specific formation of a disulfide bond. We demonstrate that this template-catalyzed strategy provides a consistent and reproducible means to conjugate fluorescent dyes, cytotoxins, or "click" chemistry handles to meditope-enabled mAbs (memAbs) and memFabs...
May 15, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29762014/selective-and-sensitive-pull-down-of-amyloid-fibrils-produced-in-vitro-and-in-vivo-by-the-use-of-pentameric-thiophene-coupled-resins
#15
Anna Beatriz Wreden, Luiza Fernandes, Mirian Kelley, Antônio Pereira-Neves, Caroline S Moreira, David R da Rocha, Fernando L Palhano
Protein aggregation is a hallmark of several degenerative diseases, including Alzheimer's disease, Parkinson's disease and familial amyloidosis (Finnish type) (FAF). A method to isolate and detect amyloids is desired for the diagnosis of amyloid diseases. Here, we report the synthesis of pentameric thiophene amyloid ligand (p-FTAA) linked to agarose resin for selective purification of amyloid aggregates produced in vitro and in vivo. Using amyloid fibrils produced in vitro from alpha-synuclein, gelsolin and Aβ1-40 and gelsolin amyloid aggregates extracted from tissue homogenates of a mouse model of FAF, we observed that p-FTAA resin was able to pull down amyloid aggregates...
May 15, 2018: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29761558/synthesis-and-characterization-of-a-deuterium-labeled-stercobilin-a-potential-biomarker-for-autism
#16
J M Coffey, A Vadas, Y Puleo, K Lewis, G Pirone, H L Rudolph, E Helms, T D Wood, A Flynn-Charlebois
Stercobilin is an end-stage metabolite of hemoglobin, a component of red blood cells. It has been found that there is a significantly lower concentration of stercobilin in the urine of people diagnosed with Autism Spectrum Disorders (ASD), suggesting potential utility as a biomarker. In vitro, we have synthesized stercobilin from its precursor bilirubin through a reduction reaction proceeded by an oxidation reaction. In addition, we have isotopically labeled the stercobilin product with deuterium using this protocol...
May 14, 2018: Journal of Labelled Compounds & Radiopharmaceuticals
https://www.readbyqxmd.com/read/29760807/quality-control-of-next-generation-sequencing-based-in-vitro-diagnostic-test-for-onco-relevant-mutations-using-multiplex-reference-materials-in-plasma
#17
Donglai Liu, Haiwei Zhou, Dawei Shi, Shu Shen, Yabin Tian, Lin Wang, Jiatao Lou, Rong Cong, Juan Lu, Henghui Zhang, Meiru Zhao, Shida Zhu, Zhisheng Cao, Ruilin Jin, Yin Wang, Xiaoni Zhang, Guohua Yang, Youchun Wang, Chuntao Zhang
Background: Widespread clinical implementation of next-generation sequencing (NGS)-based cancer in vitro diagnostic tests (IVDs) highlighted the urgency to establish reference materials which could provide full control of the process from nucleic acid extraction to test report generation. The formalin-fixed, paraffin-embedded (FFPE) tissue and blood plasma containing circulating tumor deoxyribonucleic acid (ctDNA) were mostly used for clinically detecting onco-relevant mutations. Methods: We respectively developed multiplex FFPE and plasma reference materials covering three clinically onco-relevant mutations within the epidermal growth factor receptor ( EGFR ) gene at serial allelic frequencies...
2018: Journal of Cancer
https://www.readbyqxmd.com/read/29760145/-in-vitro-activity-of-imipenem-relebactam-and-ceftolozane-tazobactam-against-resistant-gram-negative-bacilli
#18
Suzannah M Schmidt-Malan, Avisya J Mishra, Ammara Mushtaq, Cassandra L Brinkman, Robin Patel
Understanding which antimicrobial agents are likely to be active against Gram-negative bacilli can guide selection of antimicrobials for empiric therapy as mechanistic-based (i.e., molecular) rapid diagnostics are adopted. Here, we determined the MICs of the novel β-lactam/β-lactamase inhibitor combination, imipenem-relebactam, along with ceftolozane-tazobactam, imipenem, ertapenem, meropenem, ceftriaxone, and cefepime, against 283 multidrug resistant isolates of Gram-negative bacilli. For isolates harboring bla KPC (n=111), the addition of relebactam to imipenem lowered the MIC50 /MIC90 from 16/>128 μg/ml for imipenem alone to 0...
May 14, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29759377/diagnostic-diva-tests-accompanying-the-disabled-infectious-single-animal-disa-vaccine-platform-for-african-horse-sickness
#19
Piet A van Rijn, Mieke A Maris-Veldhuis, Jan Boonstra, René G P van Gennip
African Horse Sickness Virus (AHSV) (Orbivirus genus, Reoviridae family) causes high mortality in naïve domestic horses with enormous economic and socio-emotional impact. There are nine AHSV serotypes showing limited cross neutralization. AHSV is transmitted by competent species of Culicoides biting midges. AHS is a serious threat beyond the African continent as endemic Culicoides species in moderate climates transmit the closely related prototype bluetongue virus. There is a desperate need for safe and efficacious vaccines, while DIVA (Differentiating Infected from Vaccinated) vaccines would accelerate control of AHS...
May 11, 2018: Vaccine
https://www.readbyqxmd.com/read/29759186/affinity-capture-surface-carboxyl-functionalized-mos-2-sheets-to-enhance-the-sensitivity-of-surface-plasmon-resonance-immunosensors
#20
Nan-Fu Chiu, Ting-Li Lin
The development of functionalized molybdenum disulfide (MoS2 ) has led to a new trend in the biosensing field, owing to its high sensitivity and bio-affinity characteristics with regards to the simple synthesis of carboxyl-functionalized MoS2 nanocomposites. In this study, we used monochloroacetic acid (MCA) to successfully modify carboxyl-MoS2 . The efficiency of this MCA modification method showed a higher -COOH group content of 30.1%, mainly due to chlorine atoms occupying the MoS2 sulfur vacancy to allow for the formation of a strong bonding effect...
August 1, 2018: Talanta
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