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Syk cell expression

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https://www.readbyqxmd.com/read/29020462/syk-inhibitor-r406-down-regulates-inflammation-in-an-in-vitro-model-of-pseudomonas-aeruginosa-infection
#1
Alaa Alhazmi, Joshua Choi, Marina Ulanova
As Pseudomonas aeruginosa infections are characterized by strong inflammation of infected tissues anti-inflammatory therapies in combination with antibiotics have been considered for the treatment of associated diseases. Syk tyrosine kinase is an important regulator of inflammatory responses, and its specific inhibition was explored as a therapeutic option in several inflammatory conditions; however, this has not been studied in bacterial infections. We used a model of in vitro infection of human monocytic cell line THP-1 and lung epithelial cell line H292 with both wild type and flagella-deficient mutant of P...
October 11, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28948613/protein-tyrosine-phosphatase-ptpn22-regulates-il-1%C3%AE-dependent-th17-responses-by-modulating-dectin-1-signaling-in-mice
#2
Harriet A Purvis, Fiona Clarke, Christine K Jordan, Cristina Sanchez Blanco, Georgina H Cornish, Xuezhi Dai, David J Rawlings, Rose Zamoyska, Andrew P Cope
A single nucleotide polymorphism within the PTPN22 gene is a strong genetic risk factor predisposing to the development of multiple autoimmune diseases. PTPN22 regulates Syk and Src family kinases downstream of immuno-receptors. Fungal β-glucan receptor dectin-1 signals via Syk, and dectin-1 stimulation induces arthritis in mouse models. We investigated whether PTPN22 regulates dectin-1 dependent immune responses. Bone marrow derived dendritic cells (BMDCs) generated from C57BL/6 wild type (WT) and Ptpn22(-/-) mutant mice, were pulsed with OVA323-339 and the dectin-1 agonist curdlan and co-cultured in vitro with OT-II T-cells or adoptively transferred into OT-II mice, and T-cell responses were determined by immunoassay...
September 25, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28938395/aldose-reductase-mediates-nlrp3-inflammasome-initiated-innate-immune-response-in-hyperglycemia-induced-thp1-monocytes-and-male-mice
#3
Pabitra B Pal, Himangshu Sonowal, Kirtikar Shukla, Satish K Srivastava, Kota V Ramana
Despite recent studies that show oxidative stress-generated reactive oxygen species (ROS) regulate NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome-mediated innate immune response in various diabetic complications, the mechanism by which ROS activate innate immune response is not well understood. We have shown previously that aldose reductase (AR), besides reducing glucose, reduces lipid aldehydes and their glutathione conjugates and participates in various oxidative stress-induced inflammatory pathways...
October 1, 2017: Endocrinology
https://www.readbyqxmd.com/read/28928455/polydatin-inhibits-mast-cell-mediated-allergic-inflammation-by-targeting-pi3k-akt-mapk-nf-%C3%AE%C2%BAb-and-nrf2-ho-1-pathways
#4
Jing Ye, Hongmei Piao, Jingzhi Jiang, Guangyu Jin, Mingyu Zheng, Jinshi Yang, Xiang Jin, Tianyi Sun, Yun Ho Choi, Liangchang Li, Guanghai Yan
Polydatin(PD) shows anti-allergic inflammatory effect, and this study investigated its underlying mechanisms in in vitro and in vivo models. IgE-mediated passive cutaneous anaphylaxis (PCA) and passive systemic anaphylaxis (PSA) models were used to confirm PD effect in vivo. Various signaling pathway proteins in mast cell were examined. RT-PCR, ELISA and western blotting were applied when appropriate. Activity of Lyn and Fyn kinases in vitro was measured using the Kinase Enzyme System. PD dose-dependently reduced the pigmentation of Evans blue in the PCA model and decreased the concentration of serum histamine in PSA model, and attenuated the degranulation of mast cells without generating cytotoxicity...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28904407/syk-dependent-tyrosine-phosphorylation-of-3bp2-is-required-for-optimal-fcr%C3%AE-mediated-phagocytosis-and-chemokine-expression-in-u937-cells
#5
Kazuyasu Chihara, Yuji Kato, Hatsumi Yoshiki, Kenji Takeuchi, Shigeharu Fujieda, Kiyonao Sada
The adaptor protein c-Abl SH3 domain binding protein-2 (3BP2) is tyrosine phosphorylated by Syk in response to cross-linking of antigen receptors, which in turn activates various immune responses. Recently, a study using the mouse model of cherubism, a dominant inherited disorder caused by mutations in the gene encoding 3BP2, showed that 3BP2 is involved in the regulation of phagocytosis mediated by Fc receptor for IgG (FcγR) in macrophages. However, the molecular mechanisms underlying 3BP2-mediated regulation of phagocytosis and the physiological relevance of 3BP2 tyrosine phosphorylation remains elusive...
September 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28891650/asiaticoside-mitigates-the-allergic-inflammation-by-abrogating-the-degranulation-of-mast-cells
#6
Jing Zhi Jiang, Jing Ye, Guang Yu Jin, Hong Mei Piao, Hong Cui, Ming Yu Zheng, Jin Shi Yang, Nan Che, Yun Ho Choi, Liang Chang Li, Guang Hai Yan
The effects of asiaticoside (AS) on allergic responses mediated by mast cells were investigated. AS showed no obvious cytotoxicity on RPMCs (rat peritoneal mast cells). AS reduced the intracellular calcium in RPMCs and deprived the histamine release and degranulation. AS also decreased the generation of antigen-induced tumor necrosis factor α, interleukin (IL)-4, IL-8, and IL-1β in RBL-2H3 cells sensitized by IgE. The suppression of AS on pro-inflammatory cytokines was related with the activation of the intracellular FcεRI and the inhibition of the nuclear factor-κB signaling pathway...
September 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28886250/dectin-2-is-a-c-type-lectin-receptor-that-recognizes-pneumocystis-and-participates-in-innate-immune-responses
#7
Theodore J Kottom, Deanne M Hebrink, Paige E Jenson, Paige L Marsolek, Marcel Wüthrich, Huafeng Wang, Bruce Klein, Sho Yamasaki, Andrew H Limper
Pneumocystis remains an important fungal pathogen causing life-threatening pneumonia in patients with AIDS and malignancy. Lung fungal pathogens are recognized by C-type Lectin Receptors (CLRs), which bind specific ligands and stimulate innate immune responses. Previously, a role for the CLR Dectin-1 has been shown to mediate immune responses to Pneumocystis spp. For this reason, we investigated a potential role for Dectin-2. Rats with Pneumocystis pneumonia (PCP) exhibited elevated Dectin-2 mRNA levels. Soluble Dectin-2 CRD fusions protein showed binding to intact Pneumocystis carinii (Pc) and to native Pneumocystis major surface glycoprotein/glycoprotein A (Msg/gpA)...
September 8, 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/28881711/characterization-of-midostaurin-as-a-dual-inhibitor-of-flt3-and-syk-and-potentiation-of-flt3-inhibition-against-flt3-itd-driven-leukemia-harboring-activated-syk-kinase
#8
Ellen L Weisberg, Alexandre Puissant, Richard Stone, Martin Sattler, Sara J Buhrlage, Jing Yang, Paul W Manley, Chengcheng Meng, Michael Buonopane, John F Daley, Suzan Lazo, Renee Wright, David M Weinstock, Amanda L Christie, Kimberly Stegmaier, James D Griffin
Oncogenic FLT3 kinase is a clinically validated target in acute myeloid leukemia (AML), and both multi-targeted and selective FLT3 inhibitors have been developed. Spleen tyrosine kinase (SYK) has been shown to be activated and increased in FLT3-ITD-positive AML patients, and has further been shown to be critical for transformation and maintenance of the leukemic clone in these patients. Further, over-expression of constitutively activated SYK causes resistance to highly selective FLT3 tyrosine kinase inhibitors (TKI)...
August 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28869869/syk-protects-cardiocytes-against-anoxia-and-hypoglycemia-induced-injury-in-ischemic-heart-failure
#9
Guotian Yin, Xiuli Yang, Qiong Li, Zhikun Guo
Spleen tyrosine kinase (SYK), a non-receptor protein tyrosine kinase, is reported to be related to cell survival after A/H (anoxia/hypoglycemia) insult. However, the role of SYK in cardiocyte survival under A/H injury remains unclear. In this study, we aimed to gain insight into the role and molecular mechanism of SYK in cardiocytes exposed to A/H stress. The mRNA and protein expressions of SYK in H9c2 cardiocytes exposed to A/H injury, separately detected by real-time quantitative PCR and Western blot, were both robustly up-regulated...
September 1, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28857181/simultaneous-induction-of-hsp70-expression-and-degranulation-in-ige-ag-stimulated-or-extracellular-hsp70-stimulated-mast-cells-129-200
#10
Xian Li, Shiro Kanegasaki, Fansi Jin, Yifeng Deng, Jae-Ryong Kim, Hyeun Wook Chang, Tomoko Tsuchiya
BACKGROUND: In mast cells, induction of HSP70 expression during antigen stimulation has not been reported. METHODS: Mouse bone marrow derived mast cells (BMMC) were stimulated with IgE/Ag or HSP70. Induction of HSP70 expression, and signaling protein phosphorylation, were evaluated by immunoblotting. RESULTS: HSP70 expression is induced in BMMC at an early stage of IgE/Ag-dependent stimulation, some of which is released from the cells in a granule-associated form...
August 30, 2017: Allergy
https://www.readbyqxmd.com/read/28855423/plant-based-food-cyanidin-3-glucoside-modulates-human-platelet-glycoprotein-vi-signaling-and-inhibits-platelet-activation-and-thrombus-formation
#11
Yanling Yao, Yanqiu Chen, Reheman Adili, Thomas McKeown, Pingguo Chen, Guangheng Zhu, Dan Li, Wenhua Ling, Heyu Ni, Yan Yang
Background: Platelets play an important role in hemostasis, thrombosis, and atherosclerosis. Glycoprotein VI (GPVI) is a major platelet receptor that interacts with exposed collagen on injured vessel walls. Our previous studies have shown that anthocyanins (a type of natural plant pigment) attenuate platelet function; however, whether anthocyanins affect collagen-induced GPVI signaling remains unknown.Objective: The objective of this study was to explore the effects of cyanidin-3-glucoside (Cy-3-g, one of the major bioactive compounds in anthocyanins) on platelet activation and thrombosis and the GPVI signaling pathway...
October 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28855374/differential-mast-cell-outcomes-are-sensitive-to-fc%C3%AE%C2%B5ri-syk-binding-kinetics
#12
Samantha L Schwartz, Cédric Cleyrat, Mark J Olah, Peter K Relich, Genevieve K Phillips, William S Hlavacek, Keith A Lidke, Bridget S Wilson, Diane S Lidke
Crosslinking of IgE-bound FcεRI triggers multiple cellular responses, including degranulation and cytokine production. Signaling is dependent on recruitment of Syk via docking of its dual SH2 domains to phosphorylated tyrosines within the FcεRI immunoreceptor tyrosine-based activation motifs. Using single molecule imaging in live cells, we directly visualized and quantified the binding of individual mNeonGreen-tagged Syk molecules as they associated with the plasma membrane after FcεRI activation. We found that Syk colocalizes transiently to FcεRI and that Syk-FcεRI binding dynamics are independent of receptor aggregate size...
August 30, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28840181/measles-virus-nucleocapsid-protein-modulates-the-signal-regulatory-protein-%C3%AE-1-sirp%C3%AE-1-to-enhance-osteoclast-differentiation-in-paget-s-disease-of-bone
#13
Kumaran Sundaram, Yuvaraj Sambandam, Srinivasan Shanmugarajan, D Sudhaker Rao, Sakamuri V Reddy
Paget's disease of bone (PDB) is a chronic localized bone disorder in an elderly population. Environmental factors such as paramyxovirus are implicated in PDB and measles virus nucleocapsid protein (MVNP) has been shown to induce pagetic osteoclasts (OCLs). However, the molecular mechanisms underlying MVNP stimulation of OCL differentiation in the PDB are unclear. We therefore determined the MVNP regulated gene expression profiling during OCL differentiation. Agilent microarray analysis of gene expression identified high levels of SIRPβ1 (353-fold) expression in MVNP transduced human bone marrow mononuclear cells stimulated with RANKL...
December 2017: Bone Reports
https://www.readbyqxmd.com/read/28830213/syk-mediated-suppression-of-inflammatory-responses-by-cordyceps-bassiana
#14
Woo Seok Yang, Gyeong Sug Nam, Mi-Yeon Kim, Jae Youl Cho
The fruit body of artificially cultivated Cordyceps bassiana has been reported to exhibit anti-inflammatory and anticancer activities. Although it has been suggested that the fruit body has neutraceutic and pharmaceutic biomaterial potential, the exact anti-inflammatory molecular mechanism has not been fully elucidated. In this study, we demonstrated the immunopharmacologic activity of Cordyceps bassiana under in vitro conditions and investigated its anti-inflammatory mechanism. Water extract (Cm-WE) of the fruit body of artificially cultivated Cordyceps bassiana without polysaccharide fractions reduced the expression of the proinflammatory genes cyclooxygenase (COX)-2, interleukin (IL)-12, and inducible nitric oxide synthase (iNOS) and promoted the expression of the anti-inflammatory gene IL-10 in lipopolysaccharide (LPS)-treated RAW264...
2017: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/28827787/degradation-of-cofilin-is-regulated-by-cbl-aip4-and-syk-resulting-in-increased-migration-of-lmp2a-positive-nasopharyngeal-carcinoma-cells
#15
Murat R Gainullin, Ilya Yu Zhukov, Xiaoying Zhou, Yingxi Mo, Lidiia Astakhova, Ingemar Ernberg, Liudmila Matskova
Expression of cofilin is directly associated with metastatic activity in many tumors. Here, we studied the role of Latent Membrane Protein 2 A (LMP2A) of Epstein-Barr Virus (EBV) in the accumulation of cofilin observed in nasopharyngeal cancer (NPC) tumor cells. We used LMP2A transformed NPC cell lines to analyze cofilin expression. We used mutation analysis, ectopic expression and down-regulation of Cbl, AIP4 and Syk in these cell lines to determine the effect of the LMP2A viral protein on cofilin degradation and its role in the assembly of a cofilin degrading protein complex...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819788/insulin-like-growth-factor-1-activates-different-catalytic-subunits-p110-of-pi3k-in-a-cell-type-dependent-manner-to-induce-lipogenesis-dependent-epithelial-mesenchymal-transition-through-the-regulation-of-adam10-and-adam17
#16
Ga Bin Park, Daejin Kim
The activation of phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) is critical for the induction of epithelial-mesenchymal transition (EMT) by growth factors, including insulin-like growth factor 1 (IGF-1). The activation of intracellular lipogenesis provides proliferative and survival signals for cancer cells. In this study, we investigated the connection between lipogenesis-related EMT processes and IGF-1-mediated PI3K p110 isoform activation in primary (SW480 cells) and metastatic (SW620) colon carcinoma cells...
August 17, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28819423/reelin-promotes-adhesion-of-multiple-myeloma-cells-to-bone-marrow-stromal-cells-via-integrin-%C3%AE-1-signaling
#17
Liang Lin, Xinwei Zhang, Li Cao, Quanming An, Jie Hao, Yan Zhang, Rong Jin, Yingjun Chang, Xiaojun Huang, Jin Lu, Qing Ge
The close interaction between tumor cells and bone marrow stromal cells plays a crucial role in the tumorigenesis of multiple myeloma (MM). Reelin, an extracellular matrix protein, is found expressed in myeloma cells and is negatively associated with prognosis. We examined the role of Reelin in myeloma cell adhesion to bone marrow stromal cells and the signaling pathways involved. The results revealed that Reelin promoted the adhesion of myeloma cells to HS-5, a bone marrow stromal cell line, via the activation of β1 integrin...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28811826/anti-inflammatory-effect-of-piper-attenuatum-methanol-extract-in-lps-stimulated-inflammatory-responses
#18
You Jin Kim, Jeong Deok, Sunggyu Kim, Deok Hyo Yoon, Gi-Ho Sung, Adithan Aravinthan, Seungihm Lee, Mi-Nam Lee, Suntaek Hong, Jong-Hoon Kim, Young-Jin Son, Jae Youl Cho
Piper attenuatum is used as a traditional medicinal plant in India. One of the substances in P. attenuatum has been suggested to have anti-inflammatory effects. However, there is insufficient research about the anti-inflammatory mechanisms of action of P. attenuatum. The effects of P. attenuatum methanol extract (Pa-ME) on the production of inflammatory mediators nitric oxide (NO) and prostaglandin E2 (PGE2), the expression of proinflammatory genes, the translocation level of transcription factors, and intracellular signaling activities were investigated using macrophages...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28791394/identification-of-key-genes-and-pathways-associated-with-classical-hodgkin-lymphoma-by-bioinformatics-analysis
#19
Zhixing Kuang, Li Guo, Xun Li
The current study aimed to explore the mechanisms associated with classic Hodgkin lymphoma (cHL) to identify novel diagnostic and therapeutic targets. The GES12453 microarray dataset was downloaded from the Gene Expression Omnibus database; the differentially expressed genes (DEGs) between cHL samples and normal B cell samples by were identified using the limma package. Gene ontology (GO) and pathway enrichment analysis of DEGs gene were performed. Furthermore, construction and analysis of protein‑protein interaction (PPI) network was performed, and co‑expression modules of DEGs were produced...
October 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28791367/molecular-mechanisms-of-breast-cancer-metastasis-by-gene-expression-profile-analysis
#20
Tianying Zheng, Aijun Wang, Dongyan Hu, Yonggang Wang
Metastasis is the main cause of breast cancer‑related mortalities. The present study aimed to uncover the relevant molecular mechanisms of breast cancer metastasis and to explore potential biomarkers that may be used for prognosis. Expression profile microarray data GSE8977, which contained 22 stroma samples (15 were from normal breast and 7 were from invasive ductal carcinoma tumor samples), were obtained from the Gene Expression Omnibus database. Following data preprocessing, differentially expressed genes (DEGs) were selected based on analyses conducted using the linear models for microarray analysis package from R and Bioconductor software...
October 2017: Molecular Medicine Reports
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