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https://www.readbyqxmd.com/read/28820030/epidemiology-of-cardiovascular-disease-in-the-united-states-implications-for-the-perfusion-profession-a-2017-update
#1
Joseph J Sistino, David C Fitzgerald
INTRODUCTION: By analyzing epidemiological trends in cardiovascular disease (CVD), key stakeholders can make informed decisions on the future of disease burden and treatment. Accordingly, the cardiovascular perfusion community would benefit from data that would help predict future professional resource utilization. METHODS: In 2003, the changes in the number of hospital discharges for CVD from 1990 to 2000 were published, based on data from the National Center for Health Statistics (NCHS)...
September 2017: Perfusion
https://www.readbyqxmd.com/read/28819318/ultrafast-response-in-ac-driven-electrochemiluminescent-cell-using-electrochemically-active-dna-ru-bpy-3-2-hybrid-film-with-mesoscopic-structures
#2
Shota Tsuneyasu, Ryota Takahashi, Haruki Minami, Kazuki Nakamura, Norihisa Kobayashi
Electrochemiluminescence (ECL) refers to light emission induced by an electrochemical redox reaction. The stability, emission response, and light intensity of the ECL device are known to be improved by using an alternating current (AC) voltage. In this paper, an AC-driven ECL device is fabricated with DNA/Ru(bpy)3(2+) hybrid film-modified electrode. The Ru(bpy)3(2+) complex exhibits significant electrochemical reactivity in the DNA/Ru(bpy)3(2+) hybrid film prepared by electrochemical adsorption. The hybrid film contains unique micrometre-scale aggregates of Ru(bpy)3(2+) in DNA matrix...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28816799/successful-extracorporeal-life-support-in-a-pediatric-hematopoietic-stem-cell-transplant-recipient-with-periengraftment-respiratory-failure
#3
Jenny Potratz, Martina Ahlmann, Claudia Rössig, Heymut Omran, Katja Masjosthusmann
The use of extracorporeal life support (ECLS) as ultimate salvage therapy for hematopoietic stem cell transplant recipients remains controversial among oncologists and critical care specialists. Prognosis is poor, particularly after allogeneic transplantation, and literature to guide clinical decision-making is scarce. Our report describes successful ECLS in a pediatric patient undergoing allogeneic hematopoietic stem cell transplantation, who developed acute respiratory failure during severe neutropenia, followed by immediate neutrophil engraftment...
August 14, 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28811034/a-simple-microdroplet-chip-consisting-of-silica-nanochannel-assisted-electrode-and-paper-cover-for-highly-sensitive-electrochemiluminescent-detection-of-drugs-in-human-serum
#4
Yi Xiao, Linru Xu, Ping Li, Xiao-Chong Tang, Lian-Wen Qi
This paper describes a simple and miniaturized microdroplet chip (μchip) that is constructed with a silica nanochannel (SNC)-assisted electrode array and a hydrophobic paper cover (SNC&P-μchip). Vertically aligned SNCs with uniform pore size of 2-3 nm in diameter and negatively charged surface can significantly accelerate the mass transport of the positively charged tris(2,2'-bipyridyl) ruthenium (II), resulting in a remarkably enhanced electrochemiluminescence (ECL) signal. The SNC-assisted electrode array was coupled to a low cost paper cover to achieve simultaneous detection of six samples in 1 min...
August 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28811023/agnps-3d-nanostructure-enhanced-electrochemiluminescence-of-cdse-quantum-dot-coupled-with-strand-displacement-amplification-for-sensitive-biosensing-of-dna
#5
Meng Jiao, Guifen Jie, Lu Tan, Shuyan Niu
A novel strategy using Ag nanoparticles-3D (AgNPs-3D) nanostructure enhanced electrochemiluminescence (ECL) of CdSe quantum dots (QDs) coupled with strand displacement amplification (SDA) for sensitive biosensing of DNA was successfully designed. The prepared CdSe QDs with intense ECL were assembled on the poly (diallyldimethylammonium chloride) (PDDA) graphene oxide (GO) nanocomposites modified electrode, then gold nanoparticles (NPs) as the quenching probe was conjugated to the QDs, ECL signal was efficiently quenched...
August 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28809974/in-situ-generation-of-electrochemiluminescent-dna-nanoflowers-as-a-signal-tag-for-mucin-1-detection-based-on-a-strategy-of-target-and-mimic-target-synchronous-cycling-amplification
#6
Sheng-Kai Li, An-Yi Chen, Xiao-Xue Niu, Zhi-Ting Liu, Min Du, Ya-Qin Chai, Ruo Yuan, Ying Zhuo
A sensitive electrochemiluminescent (ECL) aptasensor consisting of a novel ECL signal tag of DNA nanoflowers (DNA NFs) and a highly efficient target conversion strategy for the MUC1 assay was developed, which not only increased the stability for luminophore loading, but also greatly improved the detection sensitivity.
August 15, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28800507/ultrasensitive-electrochemiluminescence-immunoassay-for-simultaneous-determination-of-ca125-and-ca15-3-tumor-markers-based-on-pamam-sulfanilic-acid-ru-bpy-3-2-and-pamam-cdte-cds-nanocomposite
#7
Bahareh Babamiri, Rahman Hallaj, Abdollah Salimi
A multiplex ultrasensitive electrochemiluminescence (ECL) immunoassay was developed for the simultaneous determination of two different tumor markers, cancer antigen 153 (CA 15-3) and cancer antigen 125 (CA 125) using polyamidoamine dendrimer-quantum dots (PAMAM-QDs) and PAMAM-sulfanilic acid-Ru(bpy)3(2+) as the signal probes and Fe3O4-SiO2 as a magnetic bead. The CdTe@CdS- QDs and Ru(bpy)3(2+) at the presence of tripropyl amine (TPA) as coreactant generate ECL at an applied voltage of + 1.2V (vs Ag/AgCl) in two different wavelengths 500 and 620nm, respectively...
July 28, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28800506/ni-oh-2-ngqds-based-electrochemiluminescence-immunosensor-for-prostate-specific-antigen-detection-by-coupling-resonance-energy-transfer-with-fe3o4-mno2-composites
#8
Wenjuan Zhu, Malik Saddam Khan, Wei Cao, Xu Sun, Hongmin Ma, Yong Zhang, Qin Wei
An efficient quenching electrochemiluminescence (ECL) immunosensor based on ECL resonance energy transfer (ECL-RET) was studied for sensitive analysis of prostate specific antigen (PSA). In this protocol, nitrogen-doped graphene quantum dots (NGQDs) which could produce excellent ECL emission, were loaded onto Ni(OH)2 with the three-dimensional (3D) hierarchical and stacked lamellar structure. Taking advantages of high conductivity and large specific surface area of Ni(OH)2, the better electrochemical and ECL behavior of Ni(OH)2/NGQDs were presented...
August 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28799626/extubation-in-patients-undergoing-extracorporeal-life-support
#9
Amélie Bataillard, Amélie Hebrard, Lucie Gaide-Chevronnay, Cécile Martin, Michel Durand, Albaladejo Pierre, Jean Francois Payen
PURPOSE: Extracorporeal life support (ECLS) is a cardiopulmonary support system used for the treatment of severe cardiac and/or respiratory failure. Mortality is high partly because of the severity of the condition that requires support. The use of ECLS is generally associated with heavy sedation. The aim of this study was to demonstrate the feasibility of stopping sedation, allowing extubation of patients supported by ECLS. METHODS: 196 patients supported by ECLS for a period of 4 years were included...
August 7, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28797606/plasma-creatine-kinase-b-correlates-with-injury-severity-and-symptoms-in-professional-boxers
#10
Joseph Kilianski, Sophie Peeters, Jeff Debad, Joseph Mohmed, Steven E Wolf, Joseph P Minei, Ramon Diaz-Arrastia, Joshua W Gatson
INTRODUCTION: Each year in the United States, approximately 1.7 million people sustain a traumatic brain injury (TBI). Of these TBI events, about 75 percent are characterized as being mild brain injuries. Immediately following TBI, a secondary brain damage persists for hours, days, and even months. Previously, detection of neuronal and glial biomarkers have proven to be useful to predict neurological outcomes. Here, we hypothesized that creatine kinase, brain (CKBB) is a sensitive biomarker for acute secondary brain injury in professional boxers...
August 7, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28789600/cardiac-surgery-score-casus-improves-outcome-prediction-in-patients-treated-with-extracorporal-life-support-ecls
#11
Britt Hofmann, Moriz J Gmelin, Dietrich Metz, Christoph Raspé, Andreas Wienke, Hendrik Treede, Andreas Simm
INTRODUCTION: The often-unexpected necessity of extracorporeal life support (ECLS) implies that information on patients and end-organ functions at time of implantation is scarce. However, there is a need for early prognostic indicators and a score predicting the outcome. Therefore, we evaluated established laboratory parameters and widely used intensive care scores - cardiac surgery score (CASUS) and sequential organ failure assessment (SOFA) after ECLS implantation. METHODS: In this retrospective analysis, 90 consecutive adult patients with veno-arterial ECLS were included...
August 1, 2017: Perfusion
https://www.readbyqxmd.com/read/28782354/a-novel-ternary-electrochemiluminescence-system-based-on-rubrene-microrods-as-luminophore-and-pt-nanomaterials-as-coreaction-accelerator-for-ultrasensitive-detection-of-microrna-from-cancer-cells
#12
Jia-Li Liu, Zhi-Ling Tang, Ying Zhuo, Yaqin Chai, Ruo Yuan
As the only endogenous co-reactant in ECL system, the dissolved O2 was the ideal candidate due to the mild reaction and easy operation, but compared to S2O82-, the dissolved O2 with weaker redox activity suffers from the poor enhancement effect of luminophore, which restricted the further application in bioanalysis. Here, a high-intense ECL signal was gained by the employing of Pt nanomaterials as co-reaction accelerator to generate more intermediate of dissolved O2 to promote the co-reaction efficiency. Based on a new ternary ECL system of Pt nanomaterials as the co-reaction accelerator, dissolved O2 as co-reactant, and a neotype rubrene microrods as luminophore, an efficient "on-off-on" solid-state ECL switch platfrom was designed for ultrasensitive microRNA (miRNA) detection with a background reduction strategy of ferrocene-labeled single-stranded DNA (Fc-DNA) as a quencher...
August 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28782157/initial-clinical-experiences-with-novel-diagonal-ecls-system-in-pediatric-cardiac-patients
#13
Yildiz Okan, Haydin Sertac, Ozturk Erkut, Kasar Taner, Onan I Selen, Altin H Firat, Cine Nihat, Ayyildiz Pelin, Erkan Halime, Guzeltas Alper
Extracorporeal life support (ECLS) provides mechanical support following cardiac surgery when respiratory or cardiac failure occurs. The aim of this study was to analyze the safety and feasibility of the Medos Deltastream diagonal pump (DP3) ECLS system in pediatric cardiac patients. We described the technical considerations and risk factors related to the survival outcomes in 102 pediatric cardiac patients who received ECLS support between March 2011 and April 2016. We switched from the DP2 system, which was used for the initial 25 patients, to the DP3 system after November 2012...
August 2017: Artificial Organs
https://www.readbyqxmd.com/read/28776365/hollow-porous-polymeric-nanospheres-of-a-self-enhanced-ruthenium-complex-with-improved-electrochemiluminescent-efficiency-for-ultrasensitive-aptasensor-construction
#14
Anyi Chen, Min Zhao, Ying Zhuo, Yaqin Chai, Ruo Yuan
Although Ru(II)-complex-based bulk nanomaterials have received considerable attention in electrochemiluminescent (ECL) assays owing to their strong ECL signals, the ECL efficiency of these nanomaterials was quite low since the bulk nanomaterials brought about a serious inner filter effect and excess inactive emitters. Herein, hollow porous polymeric nanospheres of a self-enhanced ruthenium complex (abbreviated as Ru-HPNSs) were prepared with a polyethylenimine-ruthenium complex precursor to greatly decrease the inner filter effect and minimize inactive emitters, which significantly improved the ECL efficiency...
August 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28761326/concentrations-of-muc16-and-muc5ac-using-three-tear-collection-methods
#15
Anna F Ablamowicz, Jason J Nichols
PURPOSE: To determine the optimal tear collection method for analysis of ocular surface mucins MUC5AC and MUC16. METHODS: Fifteen subjects without ocular surface disease were recruited. Subjects presented for tear collection on three separate days for three different tear collection methods with the order of method randomized. Methods used to collect tears from right and left eyes included Schirmer's strip, basal tear collection, and flush tear collection. All samples from the right eyes were individually analyzed for MUC5AC whereas the left eye samples were individually analyzed for MUC16...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28759057/recent-trends-in-electrochemiluminescence-aptasensors-and-their-applications
#16
Yasamin Nasiri Khonsari, Shiguo Sun
Aptamers are single stranded DNA or RNA ligands which can be selected for different targets from proteins to small organic dyes. In the past few years great progress has been accomplished in the development of aptamer based bioanalytical assays with different detection techniques. Among them, electrochemiluminescence (ECL) aptasensors are very promising because they have the advantages of both electrochemical and chemiluminescence biosensors, such as high sensitivity, low background, cost effectiveness, and ease of control...
July 31, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28743084/graphene-oxide-gold-nanorods-based-multiple-assisted-electrochemiluminescence-signal-amplification-strategy-for-sensitive-detection-of-prostate-specific-antigen
#17
Jun-Tao Cao, Jiu-Jun Yang, Li-Zhen Zhao, Yu-Ling Wang, Hui Wang, Yan-Ming Liu, Shu-Hui Ma
A novel and competitive electrochemiluminescence (ECL) aptasensor for prostate specific antigen (PSA) assay was constructed using gold nanorods functionalized graphene oxide (GO@AuNRs) multilabeled with glucose oxidase (GOD) and streptavidin (SA) toward luminol-based ECL system. A strong initial ECL signal was achieved by electrodeposited gold (DpAu) on the electrode because of gold nanoparticles (AuNPs) motivating the luminol ECL signal. The signal probes prepared by loading GOD and SA-biotin-DNA on GO@AuNRs were used for achieving multiple signal amplification...
July 20, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28739428/novel-class-1-integrons-and-sequence-types-in-vim-2-and-vim-11-producing-clinical-strains-of-enterobacter-cloacae
#18
D De Belder, D Faccone, N Tijet, R G Melano, M Rapoport, A Petroni, C Lucero, F Pasteran, A Corso, S A Gomez
All VIM-producing Enterobacteriaceae (six Enterobacter cloacae) submitted to the Argentinian Reference Laboratory in Antimicrobial Resistance in the period 2008-13 were characterized. The isolates were referred from 6 nosocomial institutions located in 5 different cities across the country. All isolates showed carbapenem disk diffusion inhibition zones ≤22mm and synergism between a carbapenem disk and EDTA/SMA. The six isolates were PCR positive for blaVIM. Imipenem MICs were ≤1 to 8μg/ml. Typing by PFGE and MLST distinguished six pulsotypes and sequence types with blaVIM located on novel class 1 integron arrays: ECL-1: ST182, In883; ECL-2, ST90, In885; ECL-3, ST88, In346 with blaVIM-11; ECL-4, ST184, In900; ECL-5, ST749-new, In900; ECL-6, ST91 and uncharacterized In...
July 21, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28737184/a-switchable-electrochromism-and-electrochemiluminescence-bifunctional-sensor-based-on-the-electro-triggered-isomerization-of-spiropyran-layered-double-hydroxides
#19
Zhixiong Li, Yuqiong Zhou, Liuqi Peng, Dongpeng Yan, Min Wei
A bifunctional ultrathin film (UTF) electrode was fabricated based on layered double hydroxides (LDHs) and spiropyran (SP), which exhibited electrochromism and electrochemiluminescence (ECL) sensing behavior toward temperature and Zn(2+) ions.
July 24, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28734366/a-label-free-genetic-biosensor-for-diabetes-based-on-aunps-decorated-ito-with-electrochemiluminescent-signaling
#20
Suyan Zhai, Chen Fang, Jilin Yan, Qun Zhao, Yifeng Tu
The variation of I27L gene closely relates to increasing risk of type 2 diabetes, thus it is greatly significant to develop various methods for its identification or monitoring. We report here a novel label-free electrochemiluminescent (ECL) biosensor for simple and effective determination of I27L gene based on Au nanoparticles functionalized ITO electrode. The ECL technique is employed to monitor the hybridization of DNA strands by measuring the changes of its intensity. Here, the ECL signal was quenched by blocked access of probe (luminol anion) owing to the electrostatic repulsion of negatively charged sensor surface and the space resistance...
August 22, 2017: Analytica Chimica Acta
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