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Yan-Mei Lei, Bai-Qi Xiao, Wen-Bin Liang, Ya-Qin Chai, Ruo Yuan, Ying Zhuo
It is well known that the conventional electrochemiluminescence (ECL) biosensor rely on the heterogeneous assay formats that involves the immobilization of biorecognition probe on the electrode surface before signal collection, which inevitably cause the efficiency of bio-recognition reactions to be limited owing to the existence of local steric hindrance. Herein, a robust, magnetic, and self-accelerated ECL nanosensor based on the multifunctionalized cobalt ferrite magnetite nanoparticles (CoFe2 O4 MNPs) was firstly designed for copper ion (Cu2+ ) detection in quasi-homogeneous system...
March 7, 2018: Biosensors & Bioelectronics
Michael Untch, Gunter von Minckwitz, Bernd Gerber, Christian Schem, Mahdi Rezai, Peter A Fasching, Hans Tesch, Holm Eggemann, Claus Hanusch, Jens Huober, Christine Solbach, Christian Jackisch, Georg Kunz, Jens-Uwe Blohmer, Maik Hauschild, Tanja Fehm, Valentina Nekljudova, Sibylle Loibl
Purpose The GeparQuinto phase III trial demonstrated a lower pathologic complete response (pCR; pT0 ypN0) rate when lapatinib was added to standard anthracycline-taxane chemotherapy compared with trastuzumab in patients with human epidermal growth factor receptor 2 (HER2) -positive breast cancer. Here, we report the long-term outcomes. Methods Patients with HER2-positive tumors (n = 615) received neoadjuvant treatment with epirubicin (E) plus cyclophosphamide (C), followed by docetaxel (T) in combination with either lapatinib (L) or trastuzumab (H; ECH-TH arm: n = 307; ECL-TL arm: n = 308)...
March 15, 2018: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
Artur Lik, Sangeth Jenthra, Lars Fritze, Lars Müller, Khai-Nghi Truong, Holger Helten
The application of our newly developed B-C coupling method by catalytic Si/B exchange is demonstrated for the synthesis of a series of triarylboranes (1), monodisperse thienyl- and furylborane dimers (2) and trimers (9), extended oligomers (3) and polymers (3'), as well as mixed (oligo)thienyl-/furylboranes. The structures of 1aaTip, 1bbTip, and 2bbbMes*, determined by X-ray crystallography, reveal largely coplanar hetarene rings and BR3 environments, being most pronounced in the furylborane species. Photophysical investigations, supported by TD-DFT calculations, revealed pronounced pi-electron delocalization over the hetarene backbones including the boron centers...
March 15, 2018: Chemistry: a European Journal
Jecca Rhea Steinberg, Janine S Bruce, Paloma Marin-Nevarez, Kimmy Phan, Sylvia Bereknyei Merrell, Lisa J Chamberlain
OBJECTIVE: School readiness by kindergarten entry is associated with increased high school graduation, decreased juvenile arrest, and better long-term health. Inadequate early childhood learning (ECL) disproportionately affects low-income children. Pediatricians have near-universal access to children younger than 5 years but remain an underused ECL resource. This study examined caregivers' perceptions of ECL, the role of the pediatrician and pediatric office, and the use of community-based ECL resources among diverse, low-income caregivers whose children were not enrolled in preschool...
March 13, 2018: Journal of Developmental and Behavioral Pediatrics: JDBP
Elizabeth A Walker, A Joshua Roberts, Erin L Louie, William E Dager
Systemic anticoagulation with unfractionated heparin is standard of care for patients receiving extracorporeal life support (ECLS); however, an alternative anticoagulant may be necessary when challenges with heparin therapy arise. Evidence for alternative anticoagulation in ECLS patients is limited. This retrospective analysis evaluated the dosing and outcomes associated with bivalirudin use in 14 adult ECLS patients. Indications for bivalirudin included heparin-induced thrombocytopenia, heparin resistance, or persistent clotting or bleeding while on heparin...
March 13, 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
David Macku, Pavel Hedvicak, John Quinn, Vladimir Bencko
Due to the hybrid warfare currently experienced by multiple NATO coalition and NATO partner nations, the tactical combat casualty care (TCCC) paradigm is greatly challenged. One of the major challenges to TCCC is the ad hoc extension phase in resource-poor environments, referred to as prolonged field care (PFC) and forward resuscitative care (FRC). The nuanced clinical skills with limited resources required by warfighters and auxiliary health care professionals to mitigate death on the battlefield and prevent morbidity and mortality in the PFC phase represent a balance that is still under review...
2018: Journal of Special Operations Medicine: a Peer Reviewed Journal for SOF Medical Professionals
Gerry Capatos, Christopher R Burke, Mark T Ogino, Roberto R Lorusso, Thomas V Brogan, D Michael McMullan, Heidi J Dalton
AIM: As experience with extracorporeal life support (ECLS) increases, indications for its use have expanded to diverse patient populations, including those with HIV infection. Pneumocystis jirovecii pneumonia (PJP) is a particularly devastating complication of HIV infections. The objective of this study was to review ECLS use in HIV-positive patients, with particular emphasis on those with concomitant PJP infection. METHODS: All patients were treated by the same ECLS team, consisting of an ECLS specialist intensivist, cardiothoracic surgeon and allied medical professionals at three healthcare institutions...
March 1, 2018: Perfusion
Annemieke Oude Lansink-Hartgring, Dinis Dos Reis Miranda, Dirk W Donker, Jacinta J Maas, Thijs Delnoij, Marijn Kuijpers, Judith van den Brule, Erik Scholten, Hendrik Endeman, Alexander P J Vlaar, Walter M van den Bergh
BACKGROUND: Extracorporeal life support (ECLS) is used to support the cardiorespiratory function in case of severe cardiac and/or respiratory failure in critically ill patients. According to the ELSO guidelines ECLS should be considered when estimated mortality risk approximates 80%. ECLS seems an efficient therapy in terms of survival benefit, but no undisputed evidence is delivered yet. The aim of the study is to assess the health-related quality of life after ECLS treatment and its cost effectiveness...
March 9, 2018: BMC Health Services Research
Jing Lu, Lin Wu, Yufang Hu, Sui Wang, Zhiyong Guo
In this study, a novel electrochemiluminescence (ECL) biosensor for sensitive detection of femtomolar miRNA-141 was constructed on the basis of Faraday cage-type strategy via graphene oxide (GO) and hybridization chain reaction (HCR)-assisted cascade amplification. A capture probe (CP) was immobilized on Fe3 O4 @SiO2 @Au nanoparticles as capture unit, which could catch the miRNA-141, and the immobilization of the signal unit (Ru(phen)3 2+ -HCR/GO) was allowed via nucleic acid hybridization. The prepared biosensor exhibited two advantages for signal amplification: firstly, GO could lap on the electrode surface directly, extending Outer Helmholtz Plane (OHP) of the sensor due to the large surface area and good electronic transport property; secondly, HCR-assisted cascade amplification was designed by anchoring all HCR products on the GO surface, then embedding Ru(phen)3 2+ as a signal readout pathway...
March 6, 2018: Biosensors & Bioelectronics
Seol Baek, Seung-Ryong Kwon, Song Yi Yeon, Sun-Heui Yoon, Chung Mu Kang, Seok Hee Han, Dahye Lee, Taek Dong Chung
We suggest electrochemiluminescence (ECL) sensing platform driven by eco-friendly, disposable, and miniaturized reverse elec-trodialysis (RED) patches as an electric power source. The flexible RED patches composed of ion-exchange membranes (IEMs) can produce voltage required for ECL sensing by simply choosing the appropriate number of the IEMs and the ratio of salt con-centrations. We integrate the RED patch with a bipolar electrode on the microfluidic chip to demonstrate the proof-of-concept, i.e. glucose detection in the range of 0...
March 9, 2018: Analytical Chemistry
Mohit Parekh, Davide Borroni, Alessandro Ruzza, Hannah J Levis, Stefano Ferrari, Diego Ponzin, Vito Romano
PURPOSE: To compare different Descemet membrane endothelial keratoplasty (DMEK) graft preparation methods. METHODS: Stripping from the trabecular meshwork (M1) using epithelial spatula; stripping by scoring the peripheral endothelium (M2) using Sinskey hook; stripping by punch method (M3) using donor trephine; Submerged hydro-separation (M4); and pneumatic dissection method (M5) were evaluated. Preparation time, costs, endothelial cell loss (ECL) postpreparation, cell death and morphology were compared...
March 9, 2018: Acta Ophthalmologica
Jerome Soquet, Roberto Chiletti, Stephen Horton, Igor E Konstantinov, Johann Brink, Christian P Brizard, Warwick Butt, Yves d'Udekem
BACKGROUND: In 2011 we reported unfavourable outcomes of second-run extracorporeal life support (ECLS) in children. We wanted to investigate whether this previous report affected our strategy and modified our long-term outcomes. METHODS: Between 1988 and 2015, 31 patients underwent a second-run ECLS. Median age at the time of first support was 9days (0-16 years). Median length of support for the first and second runs were 4.7days (0.1-10) and 3.6days (0.5-8.7) respectively, with an interval of 1...
February 13, 2018: Heart, Lung & Circulation
Matteo DI Nardo, Fabio S Taccone, Justyna Swol, Leen Vercaemst, Mirko Belliato
No abstract text is available yet for this article.
March 7, 2018: Minerva Anestesiologica
Tiffany L Green, Mandar V Bodas, Heather A Jones, Saba W Masho, Nao Hagiwara
Immigrant women face unique barriers to prenatal care access and patient-provider communication. Yet, few prior studies have examined U.S.-born/immigrant differences in the content of care. The purpose of this study was to investigate the roles of immigrant status, English proficiency and race/ethnicity on the receipt of self-reported prenatal counseling using nationally representative data. We used data from the Early Childhood Longitudinal Study-Birth Cohort (N ≈ 8100). We investigated differences in self-reported prenatal counseling by immigrant status, English proficiency, and race/ethnicity using logistic regression...
March 8, 2018: Journal of Community Health
Meliha Salahuddin, Adriana Pérez, Nalini Ranjit, Deanna M Hoelscher, Steven H Kelder
BACKGROUND: The goal of this study was to assess the effect of prenatal maternal cigarette smoking on children's BMI z-score trajectories, and to evaluate whether small-for-gestational-age (SGA) acts as a potential mediator between prenatal maternal cigarette smoking and child's BMI z-score at 4 years of age. METHODS: Group-based trajectory modeling (GBTM) methods were employed to describe and classify developmental BMI z-score trajectories (the outcome of interest) in children from 9 months to 4 years of age (n = 5221) in the Early Childhood Longitudinal Study, Birth Cohort (ECLS-B) study (2001-2005)...
February 21, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
Huan Wang, Guiqiang Pu, Samrat Devaramani, Yanfeng Wang, Zhaofan Yang, Linfang Li, Xiaofang Ma, Xiaoquan Lu
How to improve the accuracy of target detection substance in low-abundance and complex of real sample, which is still a major challenge in the analysis field. There is no doubt that the internal standard method is the best choice in the analysis methods. The internal standard method of ECL strategy can furnish more accurate detection results in the changeable complex environment, and it can dispel the primary vaguest interference in the system through the self-calibration of two emission spectrums. Herein, we effectually explored a strong and stable bimodal ECL system based on g-CNQDs as single luminophore in the presence of double co-reactants potassium persulfate (K2S2O8) and tetrabutylammonium bromide (TBAB) under the optimized conditions...
March 6, 2018: Analytical Chemistry
Gen Liu, Cheng Ma, Baokang Jin, Zixuan Chen, Jun-Jie Zhu
Single-cell imaging is essential for elucidating the biological mechanism of cell function because it accurately reveals the heterogeneity among cells. The electrochemiluminescence (ECL) microscopy technique has been considered a powerful tool to study cells because of its high throughput and zero cellular background light. However, since cells are immobilized on the electrode surface, the steric hindrance and the insulation from the cells make it difficult to obtain a luminous cell ECL image. To solve this problem, direct ECL imaging of a single cell was investigated and achieved on chitosan and nano-TiO2 modified fluoride-doped tin oxide conductive glass (FTO/TiO2/CS)...
March 6, 2018: Analytical Chemistry
Tiangang Yang, Jiayuan Fu, Sijia Zheng, Huiqin Yao, Yue Jin, Yanli Lu, Hongyun Liu
In the present work, PNIPAM-DNA films were fabricated on the surface of electrodes by GOD-induced radical polymerization, where PNIPAM is poly(N-isopropylacrylamide), DNA represents natural DNA from salmon testes, and GOD is glucose oxidase. The prepared film electrodes demonstrated reversible temperature-, SO4 2- -, and pH-switched cyclic voltammetry (CV) and electrochemiluminescence (ECL) behaviors toward tris(2,2'-bipyridine)ruthenium(II) (Ru(bpy)3 2+ ) in solution. Particularly, both CV and ECL signals at 1...
February 23, 2018: Biosensors & Bioelectronics
Lajos Szentgyorgyi, Chloe Shepherd, Ken W Dunn, Peter Fawcett, Julian M Barker, Paul Exton, Marc O Maybauer
Extracorporeal membrane oxygenation (ECMO) is one of the most frequent forms of extracorporeal life support (ECLS) and can be used as rescue therapy in patients with severe respiratory failure resulting from burns and/or smoke inhalation injury. Experience and literature on this treatment option is still very limited, consequently results are varied. We report a retrospective analysis of our experience with veno-venous (VV) ECMO in burn patients. All five patients, three male and two female (age: 28-37 years) had flame type burns and smoke inhalation injury...
February 27, 2018: Burns: Journal of the International Society for Burn Injuries
Mohit Parekh, Alessandro Ruzza, Vito Romano, Elisa Favaro, Mattia Baruzzo, Gianni Salvalaio, Andrea Grassetto, Stefano Ferrari, Diego Ponzin
PURPOSE: To investigate the learning curve of Descemet membrane endothelial keratoplasty (DMEK) graft preparation in an eye bank. METHODS: Four operators prepared 645 DMEK grafts using the stripping technique between 2014 and 2017 at the Veneto Eye Bank Foundation, Italy. Endothelial cell loss (ECL) and tissue wastage were recorded retrospectively after DMEK preparation and correlated with the number of tissues prepared each year by each operator. On average, our operators performed 1 donor preparation a week over the course of this study...
March 1, 2018: Cornea
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