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Asma Aid, Samira Amokrane, Djamel Nibou, Elhadj Mekatel, Mohamed Trari, Vasile Hulea
The marine biomass Ulva compressa L. (ECL) was used as a low-cost biosorbent for the removal of Cr(VI) from contaminated aqueous solutions. The operating variables were optimized: pH ∼ 2, initial concentration of 25 mg/L, solid/liquid ratio of 6 g/L and a temperature of 50 °C, leading to an uptake elimination of 96%. A full factorial experimental design technique enabled us to obtain a mathematical model describing the Cr(VI) biosorption and to study the main effects and interactions among operational parameters...
January 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Min Tang, Zhenxian Zhou, Li Shangguan, Fang Zhao, Songqin Liu
This work proposed an electrochemiluminescence (ECL) immunosensor for quantitative monitoring of cardiac troponin I (cTnI) in plasma with soybean peroxidase (SBP) labeled-antibody as signal amplifier. The ECL sandwich immunosensor was constructed by covalent binding anti-cTnI capture antibody (Ab1) to polyethylenimine-functionalized graphene matrix, which was obtained by a simple hydrothermal reaction between polyethylenimine (PEI) and graphene oxide (GO). After that, the SBP-labeled detection antibody (SBP-Ab2), synthesized by NaIO4 method, was immobilized on the surface of electrode through sandwich immunoreaction...
April 1, 2018: Talanta
Nura Fazira Noor Azam, Sharmili Roy, Syazana Abdullah Lim, Minhaz Uddin Ahmed
Recently, there has been a growing concern of consumers on the authenticity of food ingredients including adulteration with porcine and/or its derivatives. Therefore, this work reports on the development of a novel, simple, sensitive and rapid luminol-based electrochemiluminescence (ECL) technique for Sus scrofa (Porcine) DNA detection. Porcine DNA was firstly amplified using loop-mediated isothermal amplification (LAMP) method and subsequently added to luminol solution for further ECL analysis for quantification...
May 15, 2018: Food Chemistry
Asaad G Beshish, Jeffrey D Bradley, Kelli L McDonough, Nadine L N Halligan, Walker M McHugh, Julie Sturza, Mark W Hall, Timothy T Cornell, Mary K Dahmer
Extracorporeal life support (ECLS) is a widely used lifesaving technology. Whether ECLS results in immune dysregulation is unclear. This study's aim was to examine whether ECLS affected innate immune response. All patients placed on ECLS were eligible. Blood was obtained before, during, and after ECLS. Function of the innate immune system was measured by ex vivo lipopolysaccharide (LPS)-induced tumor necrosis factor-α (TNF-α) and plasma cytokine levels (interleukin [IL]-6, IL-8, IL-10, and TNF-α). Immunoparalysis was defined as ex vivo TNF-α levels less than 200 pg/ml...
January 10, 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Han Zhang, Fumei Zuo, Xingrong Tan, Shenghao Xu, Ruo Yuan, Shihong Chen
An electrochemiluminescencent (ECL) biosensor was designed for the determination of insulin using a novel ECL resonance energy transfer (ECL-RET) strategy. In this strategy, carboxyl poly(9,9-dioctyfluorenyl-2,7-diyl) dots (PFO dots) were worked as ECL donor and 3,4,9,10-perylenetetracar-boxylic acid (PTCA) exploited as ECL acceptor, and hydrogen peroxide (H2O2) employed as the coreactant. The ECL donor and ECL acceptor were separately labeled with primary antibody (Ab1) and secondary antibody (Ab2), forming a sensing interface to the analyte target, insulin...
December 27, 2017: Biosensors & Bioelectronics
Jinmeng Xu, Yan Zhang, Li Li, Qingkun Kong, Lina Zhang, Shenguang Ge, Jinghua Yu
A highly selective two-point separation strategy was designed based on a cross-like all-in-one lab-on-paper analytical device. The stable and cleavable enzyme-coated reduced graphene oxide (rGO)-PdAu probe was fabricated as the signal reporter to enable visualization and electrochemiluminecence (ECL) dual mode sensing of Pb2+. Concretely, the experimental workflow consists of the following process, (i) fabrication of the lab-on-paper device and growth of Au NPs on ECL detection zone, (ii) immobilization of Pb2+-specific DNAzyme, (iii) hybridization between DNAzyme and rGO-PdAu-glucose oxidase (GOx) labeled oligonucleotide to form double-stranded DNA...
January 10, 2018: ACS Applied Materials & Interfaces
Mohammad Rizwan, Noor Faizah Mohd-Naim, Minhaz Uddin Ahmed
The rapid and increasing use of the nanomaterials (NMs), nanostructured materials (NSMs), metal nanoclusters (MNCs) or nanocomposites (NCs) in the development of electrochemiluminescence (ECL) nanobiosensors is a significant area of study for its massive potential in the practical application of nanobiosensor fabrication. Recently, NMs or NSMs (such as AuNPs, AgNPs, Fe₃O₄, CdS QDs, OMCs, graphene, CNTs and fullerenes) or MNCs (such as Au, Ag, and Pt) or NCs of both metallic and non-metallic origin are being employed for various purposes in the construction of biosensors...
January 9, 2018: Sensors
Shuangjiao Sun, Yanfen Wei, Hao Wang, Yupin Cao, Biyang Deng
A novel electrochemiluminescence (ECL) sensor with composite consisted of silica-sol, Zinc oxide nanoparticles (ZnO NPs), polyvinylpyrrolidone (PVP) and tris(2, 2'-bipyridine) ruthenium (II) was constructed. A new method for simultaneous determination of quinapril hydrochloride (QHCl) and its metabolite quinaprilat hydrochloride (QTHCl) in human plasma was developed using the ECL sensor coupled with capillary electrophoresis (CE). ECL intensities of QHCl and QTHCl increased dramatically when the ECL sensor was used as working electrode...
March 1, 2018: Talanta
Xiao Ling Zhang, Zhao Rong Tang, Yong Ping Dong, Cheng Ming Wang
Various kinds of ZnO nanoparticles have been successfully used in gas sensing applications, however, these nanomaterials have been rarely investigated in electrogenerated chemiluminescence (ECL). In the present work, ZnO nanorods (ZnONRs) were synthesized by hydrothermal method, and characterized by field emission scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD). ECL behaviors of ZnONRs were investigated in neutral aqueous condition in the presence of K2S2O8. Cyclic voltammetry (CV) results revealed that ZnONRs can react with K2S2O8 to generate strong light emission, revealing that K2S2O8 can act as coreactant of ZnONRs ECL...
March 1, 2018: Talanta
Asaad G Beshish, Allison Weinberg, Waseem Ostwani, Gabe E Owens
We describe the use of extracorporeal cardiopulmonary resuscitation (E-CPR) to transiently stabilize a 3-month-old patient who presented with ventricular tachyarrhythmias leading to spontaneous cardiac arrest. The patient required 4 days of extracorporeal life support (ECLS) where he was diagnosed with probable Brugada syndrome (BS). The patient was discharged home in stable condition after implantable cardioverter defibrillator placement. This case highlights the importance of early transfer to extracorporeal membrane oxygenation (ECMO) center in the setting of unexplained cardiac arrhythmia in a pediatric patient...
December 2017: Journal of Extra-corporeal Technology
Stefanie Mollborn, Elizabeth Lawrence
Health lifestyles are important for health and social identity, yet little is known about their development in early life. We use data from the Early Childhood Longitudinal Study-Kindergarten Cohort of 1998-99 (ECLS-K; N = 8,786) to track children's health lifestyles and assess a theoretical model of health lifestyle development. Latent class analyses identify health lifestyles at four time points from first to eighth grade, and multivariate models investigate their interrelationships and social contextual influences...
January 1, 2018: Journal of Health and Social Behavior
Haidong Li, Silvia Voci, Valerie Ravaine, Neso Sojic
Luminescent and redox properties of stimuli-responsive hydrogel materials have been modulated by different external stimuli which trigger swelling or collapse of the polymer matrix. There is a very rapid development in the field of such "smart" materials particularly combined with other sensing functionalities. Here, a poly(N-isopropylacrylamide) matrix incorporating covalently-bound phenylboronic acids as a saccharide sensing unit and a redox-active [Ru(bpy)3]2+ luminophore was designed and exhibited multi-stimuli responsive electrochemical and luminescent switching behaviors...
December 31, 2017: Journal of Physical Chemistry Letters
Fang-Fang Wu, Ying Zhou, Han Zhang, Ruo Yuan, Ya-Qin Chai
In this work, a luminol-centric biosensor was constructed for the ultrasensitive detection of tryptase (TPS) combining dissolved O2 as the endogenous coreactant and Au-Ag-Pt hetero-nanostructures (AAPHNs) as co-reaction accelerator. Dissolved O2 could rapidly generate superoxide anion radical (O2•-) with the catalysis of AAPHNs to in situ react with luminol anion radical (L•-) to generate excited-state species 3-aminophthalate (AP2-*) for emitting ECL signal, resulting in a remarkable "single on" state...
December 27, 2017: Analytical Chemistry
Hong Ke, Xin Zhang, Chusen Huang, Nengqin Jia
Herein, a novel methodology for ultrasensitive and facile breast cancer biomarker carbohydrate antigen 15-3 (CA15-3) evaluation was proposed by fabricating a sandwiched ECL immunosensor. In the protocol, MOCs-Fc and Pt@BSA-luminol nanohybrids were successfully synthesized and further employed to achieve dual-amplification strategy for luminol-H2O2 system. Notably, inherent porous microstructure and large specific surface area from MOCs enabled a high loading of Fc, which succeeded in catalyzing luminol-H2O2 ECL emission and therefore enhancing ECL response...
December 21, 2017: Biosensors & Bioelectronics
Lan-Lan Xu, Wei Zhang, Lei Shang, Rong-Na Ma, Li-Ping Jia, Wen-Li Jia, Huai-Sheng Wang, Li Niu
It is important to design a nice electrochemiluminescence (ECL) biological nanomaterial for fabricating sensitive ECL immunosensor to detect tumor markers. Most reported ECL nanomaterial was decorated by a number of mono-luminophore. Here, we report a novel ECL nanomaterial assembled by dual luminophores perylenetetracarboxylic acid (PTCA) and carbon quantum dots (CQDs). In the ECL nanomaterial, graphene was chosen as nanocarrier. Significant ECL intensity increases are seen in the ECL nanomaterial, which was interpreted with the proposed synergistic promotion ECL meachanism of PTCA and CQDs...
October 17, 2017: Biosensors & Bioelectronics
Elsa De La Cadena, Adriana Correa, Juan Sebastián Muñoz, Laura J Rojas, Cristhian Hernández-Gómez, Christian Pallares, Federico Perez, Robert A Bonomo, Maria V Villegas
OBJECTIVES: To examine the population structure of representative carbapenem resistant Enterobacter cloacae complex (CR-Ecl) isolates from 8 different Colombian regions and to characterize their asociated β-lactamases. METHODS: Twenty-eight CR-Ecl isolates collected in Colombia between 2009 and 2013 through the Colombian Nosocomial Network were included. Antimicrobial susceptibility testing was performed by broth microdilution. Molecular detection of carbapenemases and extended-spectrum β-lactamases (ESBL), and the presence of Tn4401 was evaluated by PCR and sequencing...
December 20, 2017: Journal of Global Antimicrobial Resistance
Mei-Xing Li, Qiu-Mei Feng, Zhen Zhou, Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
Gold nanodendrites (Au NDs) exhibit extremely strong electromagnetic field located around multiple tip branches due to plasmon coupling effect. In this work, a novel LSPR enhanced ECL emission from CdTe nanocrystals (NCs) by Au NDs for the detection of nucleic acid is reported. This system is composed of a thin film of CdTe NCs on glassy carbon electrode (GCE) as anodic ECL emitter and Au NDs as plasmon enhancer. DNA tetrahedron embedded with a stem-loop hairpin structure on one edge was ap-plied as a switch to regulate the distance between CdTe NCs and Au NDs...
December 18, 2017: Analytical Chemistry
Norihisa Shigemura
For the patients with progressively decompensating acute or acute-on-chronic respiratory failure, the first-choice treatment remains as mechanical ventilation. Despite the consistent value of mechanical ventilation, the majority of lung specialists are aware of its limitations, in particular for the patients with advanced lung failure, and inherent drawbacks that augment disease progression. More recently, the concept of allowing the lungs to 'rest and recover' has been supported by quite a few clinical studies...
December 18, 2017: General Thoracic and Cardiovascular Surgery
Hai-Jie Lu, Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
In this work, we developed a visual ECL ratiometry on a closed bipolar electrode (BPE) for the detection of prostate specific antigen (PSA), prostate cancer biomarker. High efficient CdTe QDs was synthesized which emitted visualized red light at BPE cathode. Integrating with the anodic ECL emitters, luminol, visual emission of red-blue ratiometric ECL was achieved in BPE array chips. As a sensing probe, Au NRs nanocomposite was assembled on the surface of the cathode and acted as both the quencher of the CdTe QDs ECL and the promoter of the luminol ECL...
December 6, 2017: Biosensors & Bioelectronics
Konrad Hoetzenecker, Laura Donahoe, Jonathan C Yeung, Sassan Azad, Eddy Fan, Niall D Ferguson, Lorenzo Del Sorbo, Marc de Perrot, Andrew Pierre, Kazuhiro Yasufuku, Lianne Singer, Thomas K Waddell, Shaf Keshavjee, Marcelo Cypel
OBJECTIVES: Extracorporeal life support (ECLS) is increasingly used to bridge deteriorating patients awaiting lung transplantation (LTx), however, few systematic descriptions of this practice exist. We therefore aimed to review our institutional experience over the past 10 years. METHODS: In this case series, we included all adults who received ECLS with the intent to bridge to LTx. Data were retrieved from patient charts and our institutional ECLS and transplant databases...
November 22, 2017: Journal of Thoracic and Cardiovascular Surgery
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