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https://www.readbyqxmd.com/read/28534562/k-junction-structure-mediated-exponential-signal-amplification-strategy-for-microrna-detection-in-electrochemiluminescence-biosensor
#1
Xue Li, Anyi Chen, Yaqin Chai, Ruo Yuan
Based on the novel designed K-junction structure, an economic and efficient exponential signal amplification strategy with simple protocol combining hemin/G-quadruplex, a mimetic peroxidase, as a catalyzer was proposed and utilized in an electrochemiluminescence biosensor for sensitive microRNA detection. It was noteworthy that the K-junction structure was formed with guanine-rich reporter DNA and substrate DNA modified by phosphate at the 5'-terminus. Thus, the activity of the reporter DNA could be inhibited and the recognition domain for the target microRNA constructed through the K-junction structure formed...
May 23, 2017: Analyst
https://www.readbyqxmd.com/read/28530598/understanding-the-fine-structure-of-intermediate-materials-of-maize-starches
#2
Wenfang Han, Binjia Zhang, Jiangtao Li, Siming Zhao, Meng Niu, Caihua Jia, Shanbai Xiong
Here we concern the molecular fine structure of intermediate material (IM) fraction in regular maize starch (RMS) and Starpro 40 maize starch (S40). IM had a branching degree and a molar mass (Mw) somewhere between amylopectin (AP) and amylose (AM). Compared with AP, IM had more extra-long (Fr I) and long (Fr II) chains and fb3-chains (degree of polymerization (DP)>36), with a higher average chain length (CL). Also, IM contained less A-chains but more B-chains (both BS-chains with DP 3-25 and BL-chains with DP≥26), accompanied by longer B- and BL-chains, total internal chains (TICL) and average internal chains (ICL), and a similar average external chain length (ECL)...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28522755/increased-left-ventricular-extracellular-volume-and-enhanced-twist-function-in-type-1-diabetic-individuals
#3
Zainisha Vasanji, Ronald J Sigal, Neil Derek Eves, Debra L Isaac, Matthias G Friedrich, Kelvin Chow, Richard B Thompson
Individuals with type 1 diabetes (T1D) characteristically have high glycemic levels that over time can result in reactive fibrosis and abnormalities in myocardial function. T1 mapping with magnetic resonance imaging (MRI) can estimate the extent of reactive fibrosis by measurement of the extracellular volume fraction (ECV). The extent of alterations in the ECV and associated changes in left ventricular (LV) function and morphology in individuals with T1D is unknown. Fourteen individuals with long-term T1D and fourteen sex-, age-, and body mass index-matched controls without diabetes underwent MRI measurement of myocardial T1 and ECV values as well as LV function and morphology...
May 18, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28518009/aeromedical-evacuation-using-extra-corporeal-life-support-after-resuscitated-cardiac-arrest
#4
David Gerard, Hervé Raffin, Guillaume Lebreton
BACKGROUND: Extra corporeal life support (ECLS) is presently first line therapy for refractory cardiogenic shock. Mobile circulatory support teams implant ECLS or extra corporeal membrane oxygenation (ECMO) in patients in the hospital without circulatory support. These patients are then transported to specialized centers. Here we report a case of sending a mobile circulatory support team abroad, followed by air ambulance evacuation, which, to our knowledge, has never been used as part of medical assistance abroad...
April 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28513652/an-electrochemiluminescence-assay-for-sensitive-detection-of-methyltransferase-activity-in-different-cancer-cells-by-hybridization-chain-reaction-coupled-with-a-g-quadruplex-hemin-dnazyme-biosensing-strategy
#5
Hui Zhang, Zhihui Guo, Huilei Dong, Hongfei Chen, Chenxin Cai
In this work, a highly sensitive electrochemiluminescence (ECL) assay was fabricated for the detection of human DNA (cytosine-5)-methyltransferase1 (DNMT1) activity in cancer cells. The ECL assay coupled hybridization chain reaction with a G-quadruplex/hemin DNAzyme biosensing strategy. The ECL intensity changes (ΔI) allowed detection of DNMT1 activity down to 0.09 U mL(-1), and ΔI was proportional to the logarithm of the activity of DNMT1 within the range of 1.0 to 30.0 U mL(-1) in buffer solution. It also showed high sensitivity to DNMT1 activity in A549 cells, with a detection limit of about 2 cells...
May 17, 2017: Analyst
https://www.readbyqxmd.com/read/28506218/investigation-of-the-pathophysiology-of-cardiopulmonary-bypass-using-rodent-extracorporeal-life-support-model
#6
Ru-Wen Chang, Chien-Ming Luo, Hsi-Yu Yu, Yih-Sharng Chen, Chih-Hsien Wang
BACKGROUND: Extracorporeal life support (ECLS) systems are life-saving devices used for treating patients with severe cardiopulmonary failure. In this study, we implemented a rat model of ECLS without the administration of inotropes or vasopressors. METHODS: The rats underwent 5 min of untreated asphyxial cardiac arrest and were resuscitated by ECLS for 30 min. The right external jugular vein and right femoral artery were separately cannulated to the ECLS outflow and inflow, respectively...
May 15, 2017: BMC Cardiovascular Disorders
https://www.readbyqxmd.com/read/28505395/claudin-peptidomimetics-modulate-tissue-barriers-for-enhanced-drug-delivery
#7
Sophie Dithmer, Christian Staat, Carolin Müller, Min-Chi Ku, Andreas Pohlmann, Thoralf Niendorf, Nora Gehne, Petra Fallier-Becker, Ágnes Kittel, Fruzsina R Walter, Szilvia Veszelka, Maria A Deli, Rosel Blasig, Reiner F Haseloff, Ingolf E Blasig, Lars Winkler
The blood-brain barrier (BBB) formed by the microvascular endothelium limits cerebral drug delivery. The paraendothelial cleft is sealed by tight junctions (TJs) with a major contribution from claudin-5, which we selected as a target to modulate BBB permeability. For this purpose, drug-enhancer peptides were designed based on the first extracellular loop (ECL) of claudin-5 to allow transient BBB permeabilization. Peptidomimetics (C5C2 and derivatives, nanomolar affinity to claudin-5) size-selectively (≤40 kDa) and reversibly (12-48 h) increased the permeability of brain endothelial and claudin-5-transfected epithelial cell monolayers...
May 15, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28503856/pyrazole-4-3-h-quinoline-ligand-based-iridium-iii-complexes-for-electrochemiluminescence
#8
Luisa De Cola, Chien-Wei Hsu, Elena Longhi, Stephan Sinn, Chris Hawes Hawes, David Young, Paul Kruger
Electrochemiluminescence (ECL) based on [Ru(bpy)3]2+ systems is widely utilized for immunoassays. In order to extend the promising potential of ECL based applications, Ir(III) complexes have recently attracted attention as probes because of their excellent luminescent properties and tunable emission wavelength. Here we describe a series of Ir complexes using a large π-conjugated ligand and different ancillary chelates. The complexes synthesized have been chemically and spectroscopically characterized and used for ECL measurements with annihilation and co-reactant methods...
May 14, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28502521/-extracorporeal-life-support-in-thoracic-surgery-what-are-the-indications-and-the-pertinence
#9
REVIEW
J Reeb, A Olland, S Renaud, O Collange, X Delabranche, N Santelmo, F Meziani, P-M Mertes, G Massard, P-E Falcoz
INTRODUCTION: In thoracic surgery, extracorporeal life support (ECLS) technologies are used in cases of severe and refractory respiratory failure or as intraoperative cardiorespiratory support. The objectives of this review are to describe the rationale of ECLS techniques, to review the pulmonary diseases potentially treated by ECLS, and finally to demonstrate the efficacy of ECLS, using recently published data from the literature, in order to practice evidence based medicine. STATE OF THE ART: ECLS technologies should only be undertaken in expert centers...
May 12, 2017: Revue des Maladies Respiratoires
https://www.readbyqxmd.com/read/28502425/resonance-energy-transfer-based-electrochemiluminescence-and-fluorescence-sensing-of-riboflavin-using-graphitic-carbon-nitride-quantum-dots
#10
Huan Wang, Qin Ma, Yanfeng Wang, Caihe Wang, Dongdong Qin, Duoliang Shan, Jing Chen, Xiaoquan Lu
Graphitic carbon nitride quantum dots (g-CNQDs) are rarely used in the field of electrochemiluminescence. In this paper, g-CNQDs have a strong and stable electrochemiluminescence (ECL) signal generated in the presence of co-reactant K2S2O8. The ECL signal of g-CNQDs was quenched by the mechanism of resonance energy transfer (RET) between donor g-CNQDs and receptor riboflavin (RF) that is proved by UV-vis absorption spectroscopy, electrochemiluminescence and fluorescence emission spectroscopy analysis technology...
June 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28501209/a-sensitive-signal-off-electrogenerated-chemiluminescence-biosensing-method-for-the-discrimination-of-dna-hydroxymethylation-based-on-glycosylation-modification-and-signal-quenching-from-ferroceneboronic-acid
#11
Yuling Zhang, Yan Li, Yingying Wei, Huiping Sun, Huan Wang
In this study, a new and sensitive signal-off electrogenerated chemiluminescence (ECL) biosensing method for the quantification of 5-hydroxymethylcytosine in DNA (5-hmC-DNA) was developed. The method achieved simple and sensitive detection of 5-hmC-DNA based on the glycosylation of 5-hmC, combining both the amplification function of gold nanoparticles (AuNPs) and the high quenching efficiency of the tris(2, 2'-ripyridine) dichlororuthenium(II) (Ru(bpy)3(2+))-ferrocene (Fc) system. First, the electrode modified with a mixture of Nafion and AuNPs was utilized as the platform for electrostatically adsorbing Ru(bpy)3(2+)(an ECL-emitting species) and assembling 5-hmC-DNA...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28498457/suvmax-of-18f-fdg-pet-ct-correlates-to-expression-of-major-chemotherapy-related-tumor-markers-and-serum-tumor-markers-in-gastric-adenocarcinoma-patients
#12
Lu Bai, Chi-Hua Guo, Yan Zhao, Jun-Gang Gao, Miao Li, Cong Shen, You-Min Guo, Xiao-Yi Duan
The expression of P53 was previously found by us significantly correlated with maximal standardized uptake value (SUVmax) in non-small cell lung cancer (NSCLC) patients. Hence, the aim of this study was to clarify the relationship between SUVmax and the status of the chemotherapy-related tumor marker expression or serum tumor markers in gastric adenocarcinoma patients. Sixty-four gastric adenocarcinoma patients who underwent 18F-FDG PET/CT prior to treatment were enrolled in this study. Immunohistochemistry was performed to detect changes of Her-2, P53 and Survivin in lesions, and electrochemiluminescence (ECL) method was used to quantify expression of serum CA72-4, CA19-9 and CEA of these patients...
May 5, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28497690/a-novel-electrochemiluminescence-sensing-platform-for-precise-analysis-of-multiple-ltbi-markers
#13
Bin Zhou, Mingyao Zhu, Peihui Yang, Peihui Yang
Latent tuberculosis infection (LTBI) is one of the major contributing factors for the high incidence of tuberculosis (TB), and the low contents of LTBI markers in human serum presents a great challenge for the diagnosis of LTBI. Here we reported a novel electrochemiluminescence (ECL) sensing platform for the precise analysis of multiple LTBI markers, interferon-gamma (IFN-γ) and interleukin (IL)-2. In this approach, self-prepared carbon quantum dots (CQDs) and luminol were integrated onto gold nanoparticles (AuNPs), which were further enriched on the surface of magnetic bead (MB) to create two solid-phase ECL nanoprobes (MB@Au@CQDs and MB@Au@luminol) for improving the detection sensitivity efficiently...
May 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28497221/evaluation-of-the-cross-reactivity-of-antidrug-antibodies-to-ct-p13-and-infliximab-reference-product-remicade-an-analysis-using-immunoassays-tagged-with-both-agents
#14
Walter Reinisch, Jørgen Jahnsen, Stefan Schreiber, Silvio Danese, Julián Panés, Alejandro Balsa, Won Park, JiSoo Kim, Jee Un Lee, Dae Hyun Yoo
BACKGROUND: During two pivotal clinical trials of the infliximab biosimilar CT-P13 (PLANETAS and PLANETRA), antidrug antibodies (ADAs) and neutralising antibodies (NAbs) were detected in the sera of patients treated with CT-P13 and the reference product (RP; Remicade). OBJECTIVE: The aim was to assess the comparability of Remicade- and CT-P13-tagged immunoassays for the detection of ADAs and NAbs using data from these trials, in order to determine the cross-reactivity of CT-P13 and RP ADAs...
May 11, 2017: BioDrugs: Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy
https://www.readbyqxmd.com/read/28497033/intrapulmonary-percussive-ventilation-as-an-airway-clearance-technique-during-venoarterial-extracorporeal-life-support-in-an-infant-with-pertussis
#15
Conrad Krawiec, Ken Ballinger, E Scott Halstead
Initiation of extracorporeal life support (ECLS) is often followed by complete opacification of pulmonary parenchyma and volume loss. The optimal mechanical ventilator management and lung recruitment strategy of a pediatric patient requiring extracorporeal membrane oxygenation is not known. We present a case of a 4-week old infant who developed a severe pertussis infection requiring ECLS. The severity of his illness and pertussis infection-associated intraluminal bronchiole obstruction made medical management challenging...
2017: Frontiers in Pediatrics
https://www.readbyqxmd.com/read/28493993/mechanism-of-down-regulation-of-na-h-exchanger-2-in-experimental-colitis
#16
Amal Ali Soleiman, Farook Thameem, Islam Khan
BACKGROUND: The Na-H exchanger [NHE] performs an electroneutral uptake of NaCl and water from the lumen of the gastrointestinal tract. There are several distinct NHE isoforms, some of which show an altered expression in the inflammatory bowel diseases (IBD). In this study, we examined a role of NHE-2 in experimental colitis. METHODS: Colitis was induced in male Sprague-Dawley rats by intra-rectal administration of trinitrobenzenesulphonic acid (TNBS). On day 6 post-TNBS, the animals were sacrificed, colonic and ileal segments were taken out, cleaned with phosphate buffered saline and used in this study...
2017: PloS One
https://www.readbyqxmd.com/read/28488439/a-high-efficient-electrochemiluminescence-resonance-energy-transfer-system-in-one-nanostructure-its-application-for-ultrasensitive-detection-of-microrna-in-cancer-cells
#17
Zhaoyang Li, Zongfan Lin, Xiaoyu Wu, Haotian Chen, Yaqin Chai, Ruo Yuan
The electrochemiluminesce (ECL) efficiency of luminous emitter can be enhanced by the means of electrochemiluminesce resonance energy transfer (ECL-RET) with a matched donor. However, generally, the donor and acceptor pairs were separated in different independent nanostructures, experiencing the challenging issues of limited energy transfer efficiency and luminous stability. Herein, we designed novel ECL-RET model within one nanostructure containing the donor of Tris (4,4'-dicarboxylicacid-2,2'-bipyridyl) ruthenium(II) dichloride (Ru(dcbpy)32+) and the acceptor of CdSe@ZnS quantum dots (QDs) for acting as the ECL emitter (QDs-Ru(dcbpy)32+), which significantly reduced the energy loss and improved the ECL efficiency of QDs because of the short path of energy transmission...
May 10, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28488075/effects-of-phacoemulsification-with-versus-without-viscoelastic-devices-on-surgical-outcomes
#18
İbrahim Taşkın, Lokman Aslan
PURPOSE: The aim of this study is to report surgical outcomes in patients undergoing phacoemulsification surgery (PE) with versus without ophthalmic viscosurgical devices (OVDs). METHODS: This is a comparative case series study. In total, 145 patients who performed PE with OVDs in 68 eyes (Group 1) and without OVD in 77 eyes (Group 2) were enrolled. A comprehensive ophthalmological examination was performed including slit-lamp, fundus examination. Best-corrected visual acuity (BCVA), intraocular pressure (IOP) specular endothelial microscopy (SM), and ultrasound pachymetry (UP) were also measured before surgery and at four-time points postoperatively...
May 9, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28482554/an-enhanced-electrochemiluminescence-sensor-modified-with-a-ru-bpy-3-2-yb2o3-nanoparticle-nafion-composite-for-the-analysis-of-methadone-samples
#19
Morteza Hosseini, Mohammad Reza Karimi Pur, Parviz Norouzi, Mohammad Reza Moghaddam, Mohammad Reza Ganjali
The sensitive interaction between Ru(bpy)3(2+) and methadone was used for the modification of a solid-state electrochemiluminescence electrode, and it was also successfully applied to determine methadone in the samples. The working electrode was made of a Yb2O3-containing modified carbon paste electrode. The presence of Yb2O3 nanoparticles in the structure of the electrode, was found to enhance its sensitizing effect towards the Ru(bpy)3(2+)-methadone electrochemiluminescence system. The electrodes with the optimal make-up revealed a linear ECL-concentration behavior from 5×10(-9)-3×10(-7)M and reached method detection limit as low as 2...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28481500/novel-single-cell-analysis-platform-based-on-a-solid-state-zinc-coadsorbed-carbon-quantum-dots-electrochemiluminescence-probe-for-the-evaluation-of-cd44-expression-on-breast-cancer-cells
#20
Youyi Qiu, Bin Zhou, Xiaojuan Yang, Dongping Long, Yan Hao, Peihui Yang
A novel single-cell analysis platform was fabricated using solid-state zinc-coadsorbed carbon quantum dot (ZnCQDs) nanocomposites as an electrochemiluminescence (ECL) probe for the detection of breast cancer cells and evaluation of the CD44 expression level. Solid-state ZnCQDs nanocomposite probes were constructed through the attachment of ZnCQDs to gold nanoparticles and then the loading of magnetic beads to amplify the ECL signal, exhibiting a remarkable 120-fold enhancement of the ECL intensity. Hyaluronic acid (HA)-functionalized solid-state probes were used to label a single breast cancer cell by the specific recognition of HA with CD44 on the cell surface, revealing more stable, sensitive, and effective tagging in comparison with the water-soluble CQDs...
May 15, 2017: ACS Applied Materials & Interfaces
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