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Sabina P W Guenther, Stefan Buchholz, Frank Born, Stefan Brunner, René Schramm, Dominik J Hoechter, Vera von Dossow, Maximilian Pichlmaier, Christian Hagl, Nawid Khaladj
OBJECTIVE: Extracorporeal life support (ECLS) emerges as a salvage option in therapy refractory cardiogenic shock but is limited to highly specialized tertiary care centers. Critically ill patients are often too unstable for conventional transport. Mobile ECLS programs for remote implantation and subsequent air or ground-based transport for patient retrieval could solve this dilemma and make full-spectrum advanced cardiac care available to patients in remote hospitals in whom shock otherwise might be fatal...
November 2017: Air Medical Journal
Shiho Tanaka, Haruka Endo, Satomi Adegawa, Ami Iizuka, Kazuhiro Imamura, Shingo Kikuta, Ryoichi Sato
Because Bombyx mori ABC transporter C2 (BmABCC2) has 1000-fold higher potential than B. mori cadherin-like protein as a receptor for Bacillus thuringiensis Cry1Aa toxin (Tanaka et al., 2013), the gate-opening ability of the latent pore under six extracellular loops (ECLs) of BmABCC2 was expected to be the reason for its higher potential (Heckel, 2012). In this study, cell swelling assays in Sf9 cells showed that BmABCC2 mutants lacking substrate-excreting activity retained receptor activity, indicating that the gate-opening activity of BmABCC2 is not responsible for Cry1Aa toxicity...
November 8, 2017: Insect Biochemistry and Molecular Biology
Jonathan H Lass, Beth Ann Benetz, David D Verdier, Loretta B Szczotka-Flynn, Allison R Ayala, Wendi Liang, Anthony J Aldave, Steven P Dunn, Tyrone McCall, Shahzad I Mian, Lisa C Navarro, Sanjay V Patel, Sudeep Pramanik, George O Rosenwasser, Kevin W Ross, Mark A Terry, Craig Kollman, Robin L Gal, Roy W Beck
Importance: Demonstrating that endothelial cell loss following Descemet stripping automated endothelial keratoplasty (DSAEK) is independent of donor cornea preservation time (PT) could increase the pool of corneal tissue available for keratoplasty. Objective: To determine whether endothelial cell loss 3 years after successful DSAEK is related to PT. Design, Setting, and Participants: A multicenter, double-masked, randomized clinical trial included 40 clinical sites (70 surgeons) in the United States, with donor corneas provided by 23 US eye banks...
November 10, 2017: JAMA Ophthalmology
Martin Geyer, Bernhard Gohrbandt, Ingo Sagoschen, Thomas Hartmann, Felix Post, Christian-Friedrich Vahl, Thomas Münzel
Extracorporeal membrane oxygenation (ECMO) and extracorporeal life support (ECLS) are an increasingly established advanced therapy for emerging severe lung and/or cardiocirculatory dysfunction or failure. Several reports have provided evidence for a potential benefit in prognosis by ECLS in cases of cardiogenic shock including cardiopulmonary resuscitation. Routine use in specialized centers reduces the incidence of negative side effects (e.g., vascular/ischemic, embolic, hemorrhagic, hemolytic and, furthermore, septic)...
November 9, 2017: Journal of Artificial Organs: the Official Journal of the Japanese Society for Artificial Organs
Tae Hee Hong, Heemoon Lee, Jae Jun Jung, Yang Hyun Cho, Kiick Sung, Ji-Hyuk Yang, Young-Tak Lee, Su Hyun Cho
Background: Extracorporeal life support (ECLS) is widely used in refractory heart or lung failure, and the demand for inter-facility transportation on ECLS is expanding. However, little is known about post-transportation outcomes, the clinical safety of such transportation, or the characteristics of the transported patients. Methods: This was a retrospective review of a 3-year, single-institution experience with inter-facility ECLS transport, as well as a comparative analysis of clinical outcomes with those of in-house patients...
October 2017: Korean Journal of Thoracic and Cardiovascular Surgery
Jiangnan Shu, Zhili Han, Tianhua Zheng, Dexin Du, Guizheng Zou, Hua Cui
Most electrochemiluminescence (ECL) studies involve single luminophore with a unique emission process, which severely limits its applications. Recently, multicolor ECL has attracted considerable interests. Herein, we report a novel nanoluminophore prepared by coating 5,10,15,20-tetrakis(4-carboxyphenyl)-porphyrin (TCPP) and N-(4-aminobutyl)-N-ethylisoluminol (ABEI) on the surface of TiO2 nanoparticles (TiO2-TCPP-ABEI), which exhibited unique potential-resolved multicolor ECL emissions using H2O2 and K2S2O8 as coreactants in an aqueous solution...
November 13, 2017: Analytical Chemistry
Michael Mayer, Shigehiko Takegami, Michael Neumeier, Simone Rink, Axel Jacobi von Wangelin, Silja Schulte, Moritz Vollmer, Axel Georg Griesbeck, Axel Duerkop, Antje Jutta Baeumner
The most efficient and commonly used electrochemilumi-nescence (ECL) emitters are luminol, [Ru(bpy)3]2+ and derivatives thereof. Luminol stands out due to its low excitation potential, yet applications are limited by the insolubility under physiological conditions. The water-soluble m-carboxy luminol was synthesized in 15% yield and exhibited high solubility under physiological conditions and afforded a four-fold ECL signal increase (vs. luminol). Entrapment in DNA-tagged liposomes enabled a DNA assay with a detection limit of 3...
November 9, 2017: Angewandte Chemie
Guizheng Zou, Xiao Tan, Xiaoyan Long, Yupeng He, Wujian Miao
A spectrum-resolved dual-color electrochemiluminescence (ECL) immunoassay was designed and implemented to simultaneously detect carcinoembryonic antigen (CEA) and alpha fetoprotein (AFP) with CdTe (λmax = 776 nm) and CdSe (λmax = 550 nm) nanocrystals (NCs) as ECL tags. The CdTe and CdSe NCs were labeled with respective probe antibodies (Ab2) of CEA and AFP, respectively, and then immobilized onto the working electrode surface via sandwich-type immunoreactions. Both CdTe and CdSe NCs within the NCs-immunocomplexes can be electrochemically reduced and simultaneously give off monochromatic ECL emissions in near-infrared and greenish region, respectively, when (NH4)2S2O8 was used as a cathodic ECL coreactant...
November 7, 2017: Analytical Chemistry
Yamin Nie, Pu Zhang, Haijun Wang, Ying Zhuo, Ya-Qin Chai, Ruo Yuan
Electrochemiluminescence (ECL) with high sensitivity and excellent controllability provides a promising approach for ultrasensitive detection of multiple biomarkers. However, the detection for multiple types of biomarkers on a single interface remains considerable challenge owing to the functional differentiation of different types of biomarkers. Herein, we utilized "on-off-on" switching, target-induced cleavage of peptide, and TdT (terminal deoxynucleoside transferase)-mediated extension successfully constructing a novel ECL biosensor for the ultrasensitive detection of microRNA-141 (miRNA-141) and matrix metalloproteinase-2 (MMP-2)...
November 3, 2017: Analytical Chemistry
Yaoguang Wang, Guanhui Zhao, Xiaojian Li, Li Liu, Wei Cao, Qin Wei
In this work, a highly-efficient competitive method-based electrochemiluminescence (ECL) immunosensor was proposed based on {[Ru(bpy)3][Cu2xNi2(1-x)(ox)3]}n (Cu/Ni/Ru) as luminophor to efficiently detect aflatoxins B1 (AFB1). Cu/Ni/Ru exhibited excellent ECL behavior. While polyethyleneimine capped silicon dioxide (PEI@SiO2) could decrease the ECL performance of Cu/Ni/Ru due that PEI could destroy the structure of Cu/Ni/Ru via producing the complex between PEI and metal ions (Cu(II)/Ni(II)), inducing the release of Ru(bpy)3(2+)...
October 18, 2017: Biosensors & Bioelectronics
Liuyi Hu, Jing Zheng, Kang Zhao, Anping Deng, Jianguo Li
In this study, a novel competition-type electrochemiluminescent (ECL) immunosensor for detecting diclofenac (DCF) was fabricated with graphene oxide coupled graphite-like carbon nitride (GO-g-C3N4) as signal probe for the first time. The ECL intensity of carboxylated g-C3N4 was significantly enhanced after being combined with graphene oxide (GO) which exhibited excellent charge-transport property. The sensing platform was constructed by multiwalled carbon nanotubes and gold nanoparticles (MWCNTs-AuNPs), which not only provided an effective matrix for immobilizing a large amount of coating antigen but also facilitated the electronic transmission rate to enhance the ECL intensity...
October 19, 2017: Biosensors & Bioelectronics
Amin Zhang, Weiwei Guo, Hong Ke, Xin Zhang, Huan Zhang, Chusen Huang, Dapeng Yang, Nengqin Jia, Daxiang Cui
A sandwich-configuration electrochemiluminescence (ECL) immunosensor based on Au star@BSA-Luminol nanocomposites for ultrasensitive determination of human chorionic gonadotropin (HCG) has been developed. In this work, nanostructured Polyaniline hydrogels (Pani) decorated with Pt nanoparticles (Pani/Pt) were utilized to construct the base of this immunosensor, greatly increasing the amount of loaded capture antibodies (Ab1) via linkage reagent glutaraldehyde (GA). The used conducting Pani/Pt nanocomposites possessed the unique features, such as large surface area, high electron transfer speed and favorable electrocatalytic activities of hydrogen peroxide, which offered a prominent platform for this sandwich-sensor and acted as efficient ECL signal amplifier also...
October 18, 2017: Biosensors & Bioelectronics
Li Li, Changchun Niu, Tian Li, Yafang Wan, Ying Zhou, Haijun Wang, Ruo Yuan, Pu Liao
Herein, a novel electrochemiluminescence (ECL) biosensor was proposed for ultrasensitive detection of laminin (LN), in which DNA dendrimer (D) as a promising nanocarrier for luminophore and DNA nanomachine as tactic for target recycling. The DNA dendrimer was synthesized by hybridization between sense and its antisense Y-shaped DNAs which were formed via reaction between single-stranded DNA (ssDNA) with a thiol group at the 5'-end and a synthesized trimeric cross-linker of tris(2-maleimidoethyl)amine. This dendrimer provided abundant double-stranded DNA (dsDNA) to achieve high loading efficiency for ECL luminophore...
October 11, 2017: Biosensors & Bioelectronics
Yuan Zhao, Maozhong Sun, Wei Ma, Hua Kuang, Chuanlai Xu
Self-assembly opens new avenues to direct the organization of nanoparticles (NPs) into discrete structures with predefined configuration and association numbers. Plasmonic NP dimers provide a well-defined system for investigating the plasmonic coupling and electromagnetic (EM) interaction in arrays of NPs. The programmability and structural plasticity of biomolecules offers a convenient platform for constructing of NP dimers in a controllable way. Plasmonic coupling of NPs enables dimers to exhibit tunable optical properties, such as surface-enhanced Raman scattering (SERS), chirality, photoluminescence, and electrochemiluminescence (ECL) properties, which can be tailored by altering the biomolecules, the building blocks with distinct compositions, sizes and morphology, the interparticle distances, as well as the geometric configuration of the constituent NPs...
November 7, 2017: Journal of Physical Chemistry Letters
Yuhui Liao, Xiaoming Zhou, Yu Fu, Da Xing
Electrochemiluminescence (ECL) has been engineered to perform various tasks in the area of immunoassays and molecular diagnosis. However, there is still substantial potential for the development of ECL assay with high efficiency to achieve trace analysis. Here, we demonstrate a poly-mer-amplified ECL assay via construction of a linear Ru(bpy)32+-polymer. This new polymer material compensates for the relatively low ECL intensity from single ECL luminophores and realizes a stable and controllable labeling process...
November 2, 2017: Analytical Chemistry
Itti Bist, Snehasis Bhakta, Di Jiang, Tia E Keyes, Aaron Martin, Robert J Forster, James F Rusling
Damage to DNA from metabolites of drugs and pollutants constitutes a major human toxicity pathway known as genotoxicity. Metabolites can react with metal ions and NADPH to oxidize DNA, or participate in SN2 reactions to form covalently linked adducts with DNA bases. Guanines are the main DNA oxidation sites, and 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG) is the initial product. Here we describe a novel electrochemiluminescent (ECL) microwell array that produces metabolites from test compounds and measures relative rates of DNA oxidation and DNA-adduct damage...
October 30, 2017: Analytical Chemistry
Francesco Saverio Sorrentino, Silvia Matteini, Aurelio Imburgia, Claudio Bonifazzi, Adolfo Sebastiani, Francesco Parmeggiani
PURPOSE: To study the effect of torsional phacoemulsification energy on corneal endothelium evaluating the relationship between changes of endothelial cells and postoperative visual acuity. METHODS: This prospective clinical observational cohort study included 50 patients with cataract who underwent torsional phacoemulsification. Sequential quantitative and qualitative morphometric endothelial cell analyses of the cornea were performed four weeks preoperatively and six weeks postoperatively using noncontact specular microscopy...
2017: PloS One
Xiaoqing Zhu, Qingfeng Zhai, Wenling Gu, Jing Li, Erkang Wang
Suitably designed electrochemiluminescence (ECL) carrying group acting as high-efficiency solid-state probe has attracted a lot of attention. Herein, molybdenum carbides with the two-dimensional ultrathin nanosheet structure on the surface and excellent conductivity were successfully employed as the nanocarriers for the capture of ECL reagent of luminol-capped Au nanoparticles (luminol-AuNPs). Notably, the luminol-AuNPs in the hybrid (luminol-AuNPs@Mo2C) exhibited enhanced ECL performance (∼6-fold) as compared to individual luminol-AuNPs because of the facilitated electron transfer process...
November 7, 2017: Analytical Chemistry
Xiaolu Guan, Yao Zhang, Mengmeng Yu, Chaoqi Ren, Yanni Gao, Bingling Yun, Yongzhen Liu, Yongqiang Wang, Xiaole Qi, Changjun Liu, Hongyu Cui, Yanping Zhang, Li Gao, Kai Li, Qing Pan, Baoshan Zhang, Xiaomei Wang, Yulong Gao
Chicken Na(+)/H(+) exchanger type I (chNHE1), a multispan transmembrane protein, is a cellular receptor of the subgroup J avian leukosis virus (ALV-J). To identify the functional determinants of chNHE1 responsible for the ALV-J receptor activity, a series of chimeric receptors was created by exchanging the extracellular loops (ECL) of human NHE1 (huNHE1) and chNHE1, and by ECL replacement with a hemagglutinin (HA) tag. These chimeric receptors were then used in binding and entry assays to map the minimal ALV-J gp85-binding domain of chNHE1...
October 25, 2017: Journal of Virology
Shengjie Ye, Ruihong Ye, Yuande Shi, Bin Qiu, Longhua Guo, Dihui Huang, Zhenyu Lin, Guonan Chen
Bisphenol A (BPA), a typical endocrine disruptor, is widely used as a key monomer in the packaging industry. Residual monomer can transfer from the package material to the food and thereby pose a risk to the health of the consumer, so determination of BPA migration is highly important for food safety control. In this study, a simple but sensitive electrochemiluminescence (ECL) biosensor, which combines the characteristics of high selectivity of an aptamer and high sensitivity of ECL, has been developed to detect BPA from package materials...
October 24, 2017: Analytical and Bioanalytical Chemistry
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