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https://www.readbyqxmd.com/read/28644005/from-electrochemistry-to-electroluminescence-development-and-application-in-ratiometric-aptasensor-for-aflatoxin-b1
#1
Long Wu, Fan Ding, Wenmin Yin, Jing Ma, Biru Wang, Axiu Nie, Heyou Han
Aflatoxin B1 (AFB1), one of the most toxic chemical carcinogens, has been widely studied. It remains challenging to develop simple, accurate and sensitive analytical methods for the detection of AFB1 in food matrices. In this work, based on dual-signaling strategy, a ratiometric aptasensor was designed and verified for the accurate and sensitive detection of AFB1. The electrochemical method was first used as a model to verify the specific interaction between AFB1 and the aptamer, in which ferrocene (Fc) and methylene blue (MB) anchored DNA sequences were acted as dual signals...
June 23, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28640589/ru-bpy-32-incorporated-luminescent-polymer-dots-double-enhanced-electrochemiluminescence-for-detection-of-single-nucleotide-polymorphism
#2
Yaqiang Feng, Feng Sun, Ningning Wang, Jianping Lei, Huangxian Ju
Encapsulating a large amount of electrochemiluminescence (ECL) active molecules into one nanoparticle for single target recognition can greatly improve the sensitivity of ECL assay. This work used luminescent conjugated polymer as a carrier to synthesize Ru(bpy)32+ doped polymer dots (RuPdots). The RuPdots with an average size of 20 nm and encapsulation of about 1354 Ru(bpy)32+ showed greatly enhanced ECL emission. Using tripropylamine as a co-reactant, the resonance energy transfer from the excited polymer dots to the encapsulated Ru(bpy)32+ was demonstrated, which led to 15...
June 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28637417/effect-of-heteropterys-aphrodisiaca-on-anxiety-and-male-exposure-of-female-mice-with-advanced-age
#3
Daniela Miranda Farias, Maria Silvia Ostetto, Rozimeri Colleti, Thais Barbosa, Jorge Aparecido Barros, Sergio Machado, Eric Murillo-Rodríguez, Susana E Moreno, André Barciela Veras
BACKGROUND: Effect of Heteropterys aphrodisiac (dog-node) on anxiety and function Of adult female wistar mice. The Project is an experiment with the use of Of H. aphrodisiac root extract, in order to observe the frequency of Sexual exposure of females exposed to the extract, quantify the effect of the extract on the Concentration of total testosterone and observe the anxiety levels of the animals Exposed. Results will be measured with the laboratory testosterone test and LCE and CA tests...
June 21, 2017: Current Clinical Pharmacology
https://www.readbyqxmd.com/read/28635254/sudoku-like-lab-on-paper-cyto-device-with-dual-enhancement-of-electrochemiluminescence-intermediates-strategy
#4
Hongmei Yang, Yan Zhang, Li Li, Lina Zhang, Feifei Lan, Jinghua Yu
This paper describes the design and construction of sudoku-like lab-on-paper platform, in which dual enhancement of reaction intermediates strategy was incorporated for multiplexed competitive electrochemiluminescence (ECL) cyto-assay. Benefit from the sudoku-like structure, integrated multifunction were obtained on such elaborately devised platform, including specific reagents storage, multiple samples immobilization, residual automatic washing, and signal collection. By utilizing semicarbazide (SE) and silver nanoparticles (AgNPs) as dual enhancers, more ECL intermediates could be obtained in the graphene quantum dots (GQDs) and peroxydisulfate system, resulting in the production of more excited state GQDs to emit light...
June 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28633196/tight-junction-strand-formation-by-claudin-10-isoforms-and-claudin-10a-10b-chimeras
#5
Susanne Milatz, Jörg Piontek, Caroline Hempel, Luca Meoli, Christoph Grohe, Anja Fromm, In-Fah M Lee, Rukeia El-Athman, Dorothee Günzel
Claudins are integral components of tight junctions (TJs) in epithelia and endothelia. When expressed in cell lines devoid of TJs, claudins are able to form TJ-like strands at contacts between adjacent cells. According to a current model of TJ strand formation, claudin protomers assemble in an antiparallel double row within the plasma membrane of each cell (cis-interaction) while binding to corresponding double rows from the neighboring cells (trans-interaction). Cis-interaction was proposed to involve two interfaces of the protomers' first extracellular segment (extracellular loop (ECL)1)...
June 20, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28633193/trictide-a-tricellulin-derived-peptide-to-overcome-cellular-barriers
#6
Jimmi Cording, Basak Arslan, Christian Staat, Sophie Dithmer, Susanne M Krug, Anneliese Krüger, Philipp Berndt, Ramona Günther, Lars Winkler, Ingolf E Blasig, Reiner F Haseloff
The majority of tight junction (TJ) proteins restrict the paracellular permeation of solutes via their extracellular loops (ECLs). Tricellulin tightens tricellular TJs (tTJs) and regulates bicellular TJ (bTJ) proteins. We demonstrate that the addition of recombinantly produced extracellular loop 2 (ECL2) of tricellulin opens cellular barriers. The peptidomimetic trictide, a synthetic peptide derived from tricellulin ECL2, increases the passage of ions, as well as of small and larger molecules up to 10 kDa, between 16 and 30 h after application to human epithelial colorectal adenocarcinoma cell line 2...
June 20, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28629994/the-effect-of-estrogen-on-tendon-and-ligament-metabolism-and-function
#7
REVIEW
D R Leblanc, M Schneider, P Angele, G Volmer, D Docheva
Tendons and ligaments are crucial structures inside the musculoskeletal system. Still many issues in the treatment of tendon diseases and injuries have yet not been resolved sufficiently. In particular, the role of estrogen-like compound (ECL) in tendon biology has received until now little attention in modern research, despite ECL being a well-studied and important factor in the physiology of other parts of the musculoskeletal system. In this review we attempt to summarize the available information on this topic and to determine many open questions in this field...
June 16, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28624620/agbr-nanoparticles-3d-nitrogen-doped-graphene-hydrogel-for-fabricating-all-solid-state-luminol-electrochemiluminescence-escherichia-coli-aptasensors
#8
Nan Hao, Xuan Zhang, Zhou Zhou, Rong Hua, Ying Zhang, Qian Liu, Jing Qian, Henan Li, Kun Wang
It is necessary to develop rapid, simple and accurate detection method for Escherichia coli (E. coli) due to its widely distributed pathogenic bacteria. Herein, we prepared AgBr nanoparticles (NPs) anchored 3D nitrogen-doped graphene hydrogel (3DNGH) nanocomposites with an exceptionally large accessible surface by a simple hydrothermal approach. The as-prepared 3DNGH porous nanocomposite not only showed better conductivity than that of 3D graphene due to introducing nitrogen element into graphene framework, but also provided a high loading volume for immobilizing luminol...
June 13, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28621839/evaluation-of-hemodynamic-performance-of-a-combined-ecls-and-crrt-circuit-in-seven-positions-with-a-simulated-neonatal-patient
#9
Elizabeth Profeta, Kaitlyn Shank, Shigang Wang, Christian O'Connor, Allen R Kunselman, Karl Woitas, John L Myers, Akif Ündar
As it is common for patients treated with extracorporeal life support (ECLS) to subsequently require continuous renal replacement therapy (CRRT), and neonatal patients encounter limitations due to lack of access points, inclusion of CRRT in the ECLS circuit could provide advanced treatment for this population. The objective of this study was to evaluate an alternative neonatal ECLS circuit containing either a Maquet RotaFlow centrifugal pump or Maquet HL20 roller pump with one of seven configurations of CRRT using the Prismaflex 2000 System...
June 16, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28621588/perioperative-single-site-veno-venous-extracorporeal-co2-removal-for-minimally-invasive-giant-bulla-resection
#10
Bassam Redwan, Christian Biancosino, Felix Giebel, Gabriele Woebker, Michael Eberlein, Servet Boeluekbas
Giant pulmonary bullae are rare and surgical management of patients with severe emphysema and advanced chronic obstructive lung disease (COPD) presenting with giant bullae can be very challenging. Previously, perioperative, two-site, high-flow, veno-venous extracorporeal membrane oxygenation (ECMO) was successfully utilized during giant bulla resection. Here we report the perioperative application of single-site, low-flow extracorporeal CO2 removal (ECCO2R) for minimally invasive thoracoscopic giant bulla resection...
June 1, 2017: Perfusion
https://www.readbyqxmd.com/read/28614156/endothelial-cell-loss-in-diabetic-and-nondiabetic-eyes-after-cataract-surgery
#11
Xu He, Vasilios F Diakonis, Yasamin Alavi, Nilufer Yesilirmak, Daniel Waren, Kendall Donaldson
PURPOSE: To assess and compare the changes in endothelial cell density in diabetic and nondiabetic patients after routine phacoemulsification cataract surgery. SETTING: Bascom Palmer Eye Institute, Miller School of Medicine, Miami, FL, USA. DESIGN: Retrospective nonrandomized cohort study. METHODS: This retrospective study included diabetic and nondiabetic patients who underwent phacoemulsification by a single surgeon (K...
June 13, 2017: Cornea
https://www.readbyqxmd.com/read/28612076/improving-ruthenium-based-ecl-through-nonionic-surfactants-and-tertiary-amines
#12
Stefanie Kirschbaum-Harriman, Axel Duerkop, Antje J Baeumner
The influence of surfactants and coreactants on Ru(bpy)3(2+) electrogenerated chemiluminescence (ECL) was investigated comparatively. Specifically, the influence that the two tertiary amines, N-butyldiethanolamine (NBEA) and 2-(dibutylamino)ethanol (DBAE) have on the ECL reaction, alone and in the presence of the two surfactants Triton™ X-100 and Zonyl® FSN, was investigated, in comparison with that of the well-studied and established coreactant tripropylamine (TPA). Experiments were conducted on poly(methyl methacrylate) substrates coated with plasma-evaporated gold as used in many miniaturized systems...
June 14, 2017: Analyst
https://www.readbyqxmd.com/read/28598607/electrochemistry-and-electrochemiluminescence-of-organometal-halide-perovskites-nanocrystals-in-aqueous-medium
#13
Xiao Tan, Bin Zhang, Guizheng Zou
The redox nature and electrochemiluminescence (ECL) of highly crystalized organometal halide perovskites CH3NH3PbBr3 nanocrystals (NCs) in aqueous medium were investigated for the first time. CH3NH3PbBr3 NCs could be electrochemically reduced to negtive charged states by injecting electrons onto the lowest unoccupied molecular orbitals and oxidized to positive chargrd states by removing electrons from the highest occupied molecular orbitals, the charge transfer between NCs of positive charged states and negative charged states could bring out ECL...
June 9, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28594160/spontaneous-pt-deposition-on-defective-surfaces-of-in2o3-nanocrystals-confined-within-cavities-of-hollow-silica-nanoshells-pt-catalyst-modified-ito-electrode-with-enhanced-ecl-performance
#14
Young Shin Cho, Soo Min Kim, Youngwon Ju, Junghoon Kim, Ki-Wan Jeon, Seung Hwan Cho, Joohoon Kim, In Su Lee
Although the deposition of metallic domains on a preformed semiconductor nanocrystal provides an effective pathway to access diverse hybrid nanocrystals with synergistic metal/semiconductor heterojunction interface, those reactions that take place on the surface of semiconductor nanoscrystals have not been investigated thoroughly, because of the impediments caused by the surface-capping organic surfactants. By exploiting the interfacial reactions occurring between the solution and nanoparticles confined with the cavities of hollow nanoparticles, we propose a novel nanospace-confined strategy for assessing the innate reactivity of surfaces of inorganic semiconductor nanoparticles...
June 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28592918/electrochemiluminescence-arrays-for-studies-of-metabolite-related-toxicity
#15
Kiran Bano, James F Rusling
This article reviews recent progress from our laboratory in electrochemiluminescence (ECL) arrays designed for screening toxicity-related chemistry of chemical and drug candidates. Cytochrome P450s and metabolic bioconjugation enzymes convert lipophilic chemicals in our bodies by oxidation and bioconjugation that can lead to toxic metabolites. DNA can be used as an easily measurable toxicity-related endpoint, targeting DNA oxidation and addcut formation with metabolites. ECL using guanosines in the DNA strands as co-reactants have been used in high throughput arrays utilizing DNA-enzyme films fabricated layer-by-layer...
November 2016: Electroanalysis
https://www.readbyqxmd.com/read/28590062/sensitivity-improvement-of-rapid-vibrio-harveyi-detection-with-an-enhanced-chemiluminescent-ecl-based-dot-blot
#16
Hui Li, Jingfan Xiao, Ya Zhou, Qiyao Wang, Yuanxing Zhang
Vibrio harveyi is an opportunistic pathogen in seawater and can cause severe vibriosis. It is prevalent in hatcheries worldwide and can lead to severe economic losses. Therefore, there is an urgent need to develop a rapid detection method for monitoring this pathogen. In this study, to increase the detection sensitivity of our assay with monoclonal antibodies (Mabs) against V. harveyi, the conditions of the dot blot assay were optimized, and enhanced chemiluminescent (ECL) substrate replaced the traditional tetramethylbenzidine (TMB) substrate...
June 7, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28590001/signal-enhancement-and-low-oxidation-potentials-for-miniaturized-ecl-biosensors-via-n-butyldiethanolamine
#17
Stefanie Kirschbaum-Harriman, Michael Mayer, Axel Duerkop, Thomas Hirsch, Antje J Baeumner
We present studies on ruthenium-based electrochemiluminescence (ECL) focusing on conditions supporting signal enhancement and low oxidation potentials. Low oxidation potentials (LOPs) are especially attractive for miniaturized ECL biosensors, as microfabricated electrodes tend to detach from their support when used with high currents and operated at high potentials. Furthermore, high potentials or current densities can lead to damage of typical biosensor surface coatings and biological probes. The possibility of generating LOP ECL signals at a potential below 900 mV was therefore studied for Ru(bpy)3(2+) with two typical coreactants, i...
June 7, 2017: Analyst
https://www.readbyqxmd.com/read/28589202/electrochemiluminescence-behavior-of-agncs-and-its-application-in-immunosensors-based-on-pani-ppy-ag-dendrite-modified-electrode
#18
Lina Zhang, Yanhu Wang, Lei Shen, Jinghua Yu, Shenguang Ge, Mei Yan
In this study, hyperbranched polyethyleneimine-protected silver nanoclusters (hPEI-AgNCs) with excellent electrochemiluminescence (ECL) emission in the presence of coreactant K2S2O8 were prepared by chemical reduction of silver ions (silver nitrate) coordinated with dendrigraft polymer, and successfully used for the construction of an ECL immunosensor. Polyaniline (PANI)/polypyrrole (PPy)-silver (Ag) dendrites with good electrical conductivity and biocompatibility were electropolymerized on the surface of indium tin oxide (ITO) electrode as carriers...
June 7, 2017: Analyst
https://www.readbyqxmd.com/read/28585414/tissue-factor-variants-induce-monocyte-transformation-and-transdifferentiation-into-endothelial-cell-like-cells
#19
G Arderiu, S Espinosa, E Peña, J Crespo, R Aledo, V Y Bogdanov, L Badimon
BACKGROUND: Monocytes (Mo) increase neovascularization by releasing proangiogenic mediators and/or transdifferentiating into endothelial cell-like (ECL). Recently, we have reported that Mo-microvascular endothelial cells (mECs) crosstalk induces mEC-tissue factor (TF) expression and promotes angiogenesis. However, the effect of TF on Mo remains unknown. OBJECTIVE: Here, we analyzed whether TF might exert angiogenic effects by inducing transdifferentiation of Mo...
June 6, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28582707/an-ultrasensitive-label-free-electrochemiluminescent-immunosensor-for-measuring-cry1ab-level-and-genetically-modified-crops-content
#20
Hongfei Gao, Luke Wen, Yuhua Wu, Zhifeng Fu, Gang Wu
The development of genetically modified (GM) insect-resistant crops has aroused great public concern about the risks on the eco-environment resulting from a release of toxic Cry proteins (such as Cry1Ab) to the soil. Therefore, it is of crucial importance to measure the Cry proteins level and the GM crops content. Here, we have tested for the first time a method that uses novel carbon nanospheres (CNPs) label-free electrochemiluminescent (ECL) immunosensor for the ultrasensitive quantification of Cry1Ab and GM crops...
April 24, 2017: Biosensors & Bioelectronics
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